               SEQUENCE LISTING

<110> Rigenerand s.r.l.

<120> Method for the production of conditionally apoptotic cells

<130> GCA1988



<150> IT102018000005951

<151> 2018-06-01

<160> 3

<170> BiSSAP 1.3.6

<210> 1
<211> 8398
<212> DNA
<213> Unknown


<220> 
<223> iCasp9 Expression vector used

<400> 1
caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac       60

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa      120

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat      180

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc      240

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga      300

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg      360

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc      420

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag      480

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc      540

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg      600

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg      660

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac      720

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac      780

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg      840

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg      900

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg      960

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     1020

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     1080

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     1140

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     1200

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     1260

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt     1320

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     1380

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     1440

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     1500

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     1560

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     1620

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     1680

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     1740

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     1800

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     1860

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     1920

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     1980

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     2040

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     2100

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt     2160

acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc     2220

cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat     2280

taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat     2340

tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg     2400

gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa     2460

cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact     2520

tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta     2580

aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt     2640

acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg     2700

ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg     2760

ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc     2820

cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt     2880

tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact     2940

agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc     3000

ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa     3060

aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agagctctct     3120

cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg     3180

agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc     3240

agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga     3300

aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca     3360

gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa     3420

ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc     3480

tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag     3540

gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg     3600

aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat     3660

tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag     3720

agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg     3780

cgcagcctca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca     3840

gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg     3900

gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca     3960

gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa     4020

tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg     4080

ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa     4140

ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa     4200

ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg     4260

cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg     4320

atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga     4380

aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg     4440

atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg     4500

ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat     4560

tacaaaaatt caaaatttta tcgatcacga gactagcctc gagaagcttg ataattcgat     4620

aattcccacg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg     4680

cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt     4740

cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc     4800

gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac     4860

gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc     4920

aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cggggcgcgc     4980

cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc     5040

cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt     5100

cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatcatc gcggccgagg     5160

caggccacgc gtatgctcga gggagtgcag gtggagacta tctccccagg agacgggcgc     5220

accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga     5280

aagaaagttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag     5340

gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa     5400

ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca     5460

catgccactc tcgtcttcga tgtggagctt ctaaaactgg aatctggcgg tggatccgga     5520

gtcgacggat ttggtgatgt cggtgctctt gagagtttga ggggaaatgc agatttggct     5580

tacatcctga gcatggagcc ctgtggccac tgcctcatta tcaacaatgt gaacttctgc     5640

cgtgagtccg ggctccgcac ccgcactggc tccaacatcg actgtgagaa gttgcggcgt     5700

cgcttctcct cgctgcattt catggtggag gtgaagggcg acctgactgc caagaaaatg     5760

gtgctggctt tgctggagct ggcgcggcag gaccacggtg ctctggactg ctgcgtggtg     5820

gtcattctct ctcacggctg tcaggccagc cacctgcagt tcccaggggc tgtctacggc     5880

acagatggat gccctgtgtc ggtcgagaag attgtgaaca tcttcaatgg gaccagctgc     5940

cccagcctgg gagggaagcc caagctcttt ttcatccagg cctgtggtgg ggagcagaaa     6000

gaccatgggt ttgaggtggc ctccacttcc cctgaagacg agtcccctgg cagtaacccc     6060

gagccagatg ccaccccgtt ccaggaaggt ttgaggacct tcgaccagct ggacgccata     6120

tctagtttgc ccacacccag tgacatcttt gtgtcctact ctactttccc aggttttgtt     6180

tcctggaggg accccaagag tggctcctgg tacgttgaga ccctggacga catctttgag     6240

cagtgggctc actctgaaga cctgcagtcc ctcctgctta gggtcgctaa tgctgtttcg     6300

gtgaaaggga tttataaaca gatgcctggt tgctttaatt tcctccggaa aaaacttttc     6360

tttaaaacat cagtcgacta tccgtacgac gtaccagact acgcactcga ctaaacgcgt     6420

gcggccgcgg ccgagcaggc cggatcctct agctagagtc gacaatcaac ctctggatta     6480

caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg     6540

atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc     6600

ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca     6660

acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac     6720

cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact     6780

catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc     6840

cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg     6900

gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc     6960

ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac     7020

gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga     7080

ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg     7140

gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc     7200

tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag     7260

cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct     7320

ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt     7380

tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag     7440

aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc     7500

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta     7560

tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc     7620

cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg     7680

agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc gtcgagacgt     7740

acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg     7800

tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt     7860

cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag     7920

cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt     7980

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt     8040

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct     8100

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg     8160

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga     8220

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc     8280

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga     8340

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcc       8398


<210> 2
<211> 1242
<212> DNA
<213> Unknown


<220> 
<223> Nucleotidic sequence of iCasp9 gene

<400> 2
atgctcgagg gagtgcaggt ggagactatc tccccaggag acgggcgcac cttccccaag      60

cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaagttgat     120

tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga     180

ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct     240

ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc     300

gtcttcgatg tggagcttct aaaactggaa tctggcggtg gatccggagt cgacggattt     360

ggtgatgtcg gtgctcttga gagtttgagg ggaaatgcag atttggctta catcctgagc     420

atggagccct gtggccactg cctcattatc aacaatgtga acttctgccg tgagtccggg     480

ctccgcaccc gcactggctc caacatcgac tgtgagaagt tgcggcgtcg cttctcctcg     540

ctgcatttca tggtggaggt gaagggcgac ctgactgcca agaaaatggt gctggctttg     600

ctggagctgg cgcggcagga ccacggtgct ctggactgct gcgtggtggt cattctctct     660

cacggctgtc aggccagcca cctgcagttc ccaggggctg tctacggcac agatggatgc     720

cctgtgtcgg tcgagaagat tgtgaacatc ttcaatggga ccagctgccc cagcctggga     780

gggaagccca agctcttttt catccaggcc tgtggtgggg agcagaaaga ccatgggttt     840

gaggtggcct ccacttcccc tgaagacgag tcccctggca gtaaccccga gccagatgcc     900

accccgttcc aggaaggttt gaggaccttc gaccagctgg acgccatatc tagtttgccc     960

acacccagtg acatctttgt gtcctactct actttcccag gttttgtttc ctggagggac    1020

cccaagagtg gctcctggta cgttgagacc ctggacgaca tctttgagca gtgggctcac    1080

tctgaagacc tgcagtccct cctgcttagg gtcgctaatg ctgtttcggt gaaagggatt    1140

tataaacaga tgcctggttg ctttaatttc ctccggaaaa aacttttctt taaaacatca    1200

gtcgactatc cgtacgacgt accagactac gcactcgact aa                       1242


<210> 3
<211> 413
<212> PRT
<213> Unknown


<220> 
<223> Protein sequence of iCasp9

<400> 3
Met Leu Glu Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      
Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          
Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              
Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  
Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  
Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      
His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ser Gly 
            100                 105                 110         
Gly Gly Ser Gly Val Asp Gly Phe Gly Asp Val Gly Ala Leu Glu Ser 
        115                 120                 125             
Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys 
    130                 135                 140                 
Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly 
145                 150                 155                 160 
Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg 
                165                 170                 175     
Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr 
            180                 185                 190         
Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Arg Gln Asp His 
        195                 200                 205             
Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln 
    210                 215                 220                 
Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys 
225                 230                 235                 240 
Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys 
                245                 250                 255     
Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly 
            260                 265                 270         
Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu 
        275                 280                 285             
Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln 
    290                 295                 300                 
Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro 
305                 310                 315                 320 
Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val 
                325                 330                 335     
Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp 
            340                 345                 350         
Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu 
        355                 360                 365             
Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met 
    370                 375                 380                 
Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
385                 390                 395                 400 
Val Asp Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Leu Asp 
                405                 410             

