                         SEQUENCE LISTING

<110>  University of Copenhagen
 
<120>  Mutant Cpf1 endonucleases

<130>  P4856PC00

<150>  18175707.1
<151>  2018-06-04

<150>  18190950.8
<151>  2018-08-27

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  3990
<212>  DNA
<213>  Francisella novicida

<400>  1
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat     3000

gctattgtgg tttttgagga tttaaatttt ggatttaaaa gagggcgttt caaggtagag     3060

aagcaggtct atcaaaagtt agaaaaaatg ctaattgaga aactaaacta tctagttttc     3120

aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct     3180

tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt     3240

tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa     3300

agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat     3360

aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc     3420

aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat     3480

cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat     3540

tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac     3600

aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca     3660

aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc     3720

tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat     3780

catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa     3840

aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac     3900

ggatccgaat tcgagctcga aaatctgtac tttcaaggcg agctccgtcg acaagcttct     3960

gctctggaac accaccatca tcatcactaa                                      3990


<210>  2
<211>  1329
<212>  PRT
<213>  Francisella novicida

<400>  2

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  Gly Ser Glu Phe Glu  Leu Glu Asn 
    1295                 1300                 1305             


Leu Tyr  Phe Gln Gly Glu Leu  Arg Arg Gln Ala Ser  Ala Leu Glu 
    1310                 1315                 1320             


His His  His His His His 
    1325                 


<210>  3
<211>  1329
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Double mutant - Nickase


<220>
<221>  PEPTIDE
<222>  (1)..(1329)
<223>  Double mutant - Nickase

<400>  3

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Gly Gly Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  Gly Ser Glu Phe Glu  Leu Glu Asn 
    1295                 1300                 1305             


Leu Tyr  Phe Gln Gly Glu Leu  Arg Arg Gln Ala Ser  Ala Leu Glu 
    1310                 1315                 1320             


His His  His His His His 
    1325                 


<210>  4
<211>  1329
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Double mutant - Non specific endonuclease


<220>
<221>  PEPTIDE
<222>  (1)..(1329)
<223>  Double mutant - Non specific endonuclease

<400>  4

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Gly Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Gly Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  Gly Ser Glu Phe Glu  Leu Glu Asn 
    1295                 1300                 1305             


Leu Tyr  Phe Gln Gly Glu Leu  Arg Arg Gln Ala Ser  Ala Leu Glu 
    1310                 1315                 1320             


His His  His His His His 
    1325                 


<210>  5
<211>  19
<212>  RNA
<213>  Artificial sequence

<220>
<223>  crRNA constant region


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  crRNA constant region

<400>  5
aauuucuacu guuguagau                                                    19


<210>  6
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  t-strand


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  t-strand

<400>  6
atgcagtggc cttattaaat gacttctcta acg                                    33


<210>  7
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nt-strand


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  nt-strand

<400>  7
cgttagagaa gtcatttaat aaggccactg cat                                    33


<210>  8
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  t-strand


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  t-strand

<400>  8
atgcagtggc cttattaaat gacttctcta acg                                    33


<210>  9
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  nt-strand


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  nt-strand

<400>  9
cgttagagaa gtcatttaat aaggccactg cat                                    33


<210>  10
<211>  43
<212>  RNA
<213>  Artificial sequence

<220>
<223>  crRNA


<220>
<221>  misc_feature
<222>  (1)..(43)
<223>  crRNA

<400>  10
aauuucuacu guuguagauc accgagugca gccacucggc gcu                         43


<210>  11
<211>  39
<212>  DNA
<213>  Francisella novicida


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  DNA sequence of REC domain

<400>  11
ttatttaagc aaattttaag tgatacagaa tctaaatct                              39


<210>  12
<211>  13
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  amino acid sequence of REC domain

<400>  12

Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
1               5                   10              


<210>  13
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  mutated REC domain, DNA sequence

<400>  13
ggtggtggag ccggtgcaag tgctggagga tctggatct                              39


<210>  14
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  mutated REC domain, amino acid sequence

<400>  14

Gly Gly Gly Ala Gly Ala Ser Ala Gly Gly Ser Gly Ser 
1               5                   10              


<210>  15
<211>  39
<212>  DNA
<213>  Francisella novicida


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  Lid domain, DNA sequence

<400>  15
gaggatttaa attttggatt taaaagaggg cgtttcaag                              39


<210>  16
<211>  13
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  Lid domain, amino acid sequence

<400>  16

Glu Asp Leu Asn Phe Gly Phe Lys Arg Gly Arg Phe Lys 
1               5                   10              


<210>  17
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  mutated Lid domain, DNA sequence

<400>  17
ggtggtggag ccggtggagc agctggaggg ggagccggt                              39


<210>  18
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  Mutated Lid domain

<400>  18

Gly Gly Gly Ala Gly Gly Ala Ala Gly Gly Gly Ala Gly 
1               5                   10              


<210>  19
<211>  36
<212>  DNA
<213>  Francisella novicida


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  finger domain

<400>  19
aaaggtataa atgaatatat aaatctatac tcacag                                 36


<210>  20
<211>  12
<212>  PRT
<213>  Francisella novicida


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  Finger domain

<400>  20

Lys Gly Ile Asn Glu Tyr Ile Asn Leu Tyr Ser Gln 
1               5                   10          


<210>  21
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  Mutated finger domain

<400>  21
ggtggtggag ccggtgcagc tggaggagct tcaggt                                 36


<210>  22
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  mutated finger domain

<400>  22

Gly Gly Gly Ala Gly Ala Ala Gly Gly Ala Ser Gly 
1               5                   10          


<210>  23
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(33)
<223>  labelled non-specific ssDNA

<400>  23
agcatgccca aattgcttac atatgtgtta cgacggt                                37


<210>  24
<211>  1307
<212>  PRT
<213>  Acidaminococcus sp. BV3L6

<400>  24

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  25
<211>  1230
<212>  PRT
<213>  Lachnospiraceae bacterium COE1

<400>  25

Met His Glu Asn Asn Gly Lys Ile Ala Asp Asn Phe Ile Gly Ile Tyr 
1               5                   10                  15      


Pro Val Ser Lys Thr Leu Arg Phe Glu Leu Lys Pro Val Gly Lys Thr 
            20                  25                  30          


Gln Glu Tyr Ile Glu Lys His Gly Ile Leu Asp Glu Asp Leu Lys Arg 
        35                  40                  45              


Ala Gly Asp Tyr Lys Ser Val Lys Lys Ile Ile Asp Ala Tyr His Lys 
    50                  55                  60                  


Tyr Phe Ile Asp Glu Ala Leu Asn Gly Ile Gln Leu Asp Gly Leu Lys 
65                  70                  75                  80  


Asn Tyr Tyr Glu Leu Tyr Glu Lys Lys Arg Asp Asn Asn Glu Glu Lys 
                85                  90                  95      


Glu Phe Gln Lys Ile Gln Met Ser Leu Arg Lys Gln Ile Val Lys Arg 
            100                 105                 110         


Phe Ser Glu His Pro Gln Tyr Lys Tyr Leu Phe Lys Lys Glu Leu Ile 
        115                 120                 125             


Lys Asn Val Leu Pro Glu Phe Thr Lys Asp Asn Ala Glu Glu Gln Thr 
    130                 135                 140                 


Leu Val Lys Ser Phe Gln Glu Phe Thr Thr Tyr Phe Glu Gly Phe His 
145                 150                 155                 160 


Gln Asn Arg Lys Asn Met Tyr Ser Asp Glu Glu Lys Ser Thr Ala Ile 
                165                 170                 175     


Ala Tyr Arg Val Val His Gln Asn Leu Pro Lys Tyr Ile Asp Asn Met 
            180                 185                 190         


Arg Ile Phe Ser Met Ile Leu Asn Thr Asp Ile Arg Ser Asp Leu Thr 
        195                 200                 205             


Glu Leu Phe Asn Asn Leu Lys Thr Lys Met Asp Ile Thr Ile Val Glu 
    210                 215                 220                 


Glu Tyr Phe Ala Ile Asp Gly Phe Asn Lys Val Val Asn Gln Lys Gly 
225                 230                 235                 240 


Ile Asp Val Tyr Asn Thr Ile Leu Gly Ala Phe Ser Thr Asp Asp Asn 
                245                 250                 255     


Thr Lys Ile Lys Gly Leu Asn Glu Tyr Ile Asn Leu Tyr Asn Gln Lys 
            260                 265                 270         


Asn Lys Ala Lys Leu Pro Lys Leu Lys Pro Leu Phe Lys Gln Ile Leu 
        275                 280                 285             


Ser Asp Arg Asp Lys Ile Ser Phe Ile Pro Glu Gln Phe Asp Ser Asp 
    290                 295                 300                 


Thr Glu Val Leu Glu Ala Val Asp Met Phe Tyr Asn Arg Leu Leu Gln 
305                 310                 315                 320 


Phe Val Ile Glu Asn Glu Gly Gln Ile Thr Ile Ser Lys Leu Leu Thr 
                325                 330                 335     


Asn Phe Ser Ala Tyr Asp Leu Asn Lys Ile Tyr Val Lys Asn Asp Thr 
            340                 345                 350         


Thr Ile Ser Ala Ile Ser Asn Asp Leu Phe Asp Asp Trp Ser Tyr Ile 
        355                 360                 365             


Ser Lys Ala Val Arg Glu Asn Tyr Asp Ser Glu Asn Val Asp Lys Asn 
    370                 375                 380                 


Lys Arg Ala Ala Ala Tyr Glu Glu Lys Lys Glu Lys Ala Leu Ser Lys 
385                 390                 395                 400 


Ile Lys Met Tyr Ser Ile Glu Glu Leu Asn Phe Phe Val Lys Lys Tyr 
                405                 410                 415     


Ser Cys Asn Glu Cys His Ile Glu Gly Tyr Phe Glu Arg Arg Ile Leu 
            420                 425                 430         


Glu Ile Leu Asp Lys Met Arg Tyr Ala Tyr Glu Ser Cys Lys Ile Leu 
        435                 440                 445             


His Asp Lys Gly Leu Ile Asn Asn Ile Ser Leu Cys Gln Asp Arg Gln 
    450                 455                 460                 


Ala Ile Ser Glu Leu Lys Asp Phe Leu Asp Ser Ile Lys Glu Val Gln 
465                 470                 475                 480 


Trp Leu Leu Lys Pro Leu Met Ile Gly Gln Glu Gln Ala Asp Lys Glu 
                485                 490                 495     


Glu Ala Phe Tyr Thr Glu Leu Leu Arg Ile Trp Glu Glu Leu Glu Pro 
            500                 505                 510         


Ile Thr Leu Leu Tyr Asn Lys Val Arg Asn Tyr Val Thr Lys Lys Pro 
        515                 520                 525             


Tyr Thr Leu Glu Lys Val Lys Leu Asn Phe Tyr Lys Ser Thr Leu Leu 
    530                 535                 540                 


Asp Gly Trp Asp Lys Asn Lys Glu Lys Asp Asn Leu Gly Ile Ile Leu 
545                 550                 555                 560 


Leu Lys Asp Gly Gln Tyr Tyr Leu Gly Ile Met Asn Arg Arg Asn Asn 
                565                 570                 575     


Lys Ile Ala Asp Asp Ala Pro Leu Ala Lys Thr Asp Asn Val Tyr Arg 
            580                 585                 590         


Lys Met Glu Tyr Lys Leu Leu Thr Lys Val Ser Ala Asn Leu Pro Arg 
        595                 600                 605             


Ile Phe Leu Lys Asp Lys Tyr Asn Pro Ser Glu Glu Met Leu Glu Lys 
    610                 615                 620                 


Tyr Glu Lys Gly Thr His Leu Lys Gly Glu Asn Phe Cys Ile Asp Asp 
625                 630                 635                 640 


Cys Arg Glu Leu Ile Asp Phe Phe Lys Lys Gly Ile Lys Gln Tyr Glu 
                645                 650                 655     


Asp Trp Gly Gln Phe Asp Phe Lys Phe Ser Asp Thr Glu Ser Tyr Asp 
            660                 665                 670         


Asp Ile Ser Ala Phe Tyr Lys Glu Val Glu His Gln Gly Tyr Lys Ile 
        675                 680                 685             


Thr Phe Arg Asp Ile Asp Glu Thr Tyr Ile Asp Ser Leu Val Asn Glu 
    690                 695                 700                 


Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Pro Tyr 
705                 710                 715                 720 


Ser Lys Gly Thr Lys Asn Leu His Thr Leu Tyr Trp Glu Met Leu Phe 
                725                 730                 735     


Ser Gln Gln Asn Leu Gln Asn Ile Val Tyr Lys Leu Asn Gly Asn Ala 
            740                 745                 750         


Glu Ile Phe Tyr Arg Lys Ala Ser Ile Asn Gln Lys Asp Val Val Val 
        755                 760                 765             


His Lys Ala Asp Leu Pro Ile Lys Asn Lys Asp Pro Gln Asn Ser Lys 
    770                 775                 780                 


Lys Glu Ser Met Phe Asp Tyr Asp Ile Ile Lys Asp Lys Arg Phe Thr 
785                 790                 795                 800 


Cys Asp Lys Tyr Gln Phe His Val Pro Ile Thr Met Asn Phe Lys Ala 
                805                 810                 815     


Leu Gly Glu Asn His Phe Asn Arg Lys Val Asn Arg Leu Ile His Asp 
            820                 825                 830         


Ala Glu Asn Met His Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu 
        835                 840                 845             


Ile Tyr Leu Cys Met Ile Asp Met Lys Gly Asn Ile Val Lys Gln Ile 
    850                 855                 860                 


Ser Leu Asn Glu Ile Ile Ser Tyr Asp Lys Asn Lys Leu Glu His Lys 
865                 870                 875                 880 


Arg Asn Tyr His Gln Leu Leu Lys Thr Arg Glu Asp Glu Asn Lys Ser 
                885                 890                 895     


Ala Arg Gln Ser Trp Gln Thr Ile His Thr Ile Lys Glu Leu Lys Glu 
            900                 905                 910         


Gly Tyr Leu Ser Gln Val Ile His Val Ile Thr Asp Leu Met Val Glu 
        915                 920                 925             


Tyr Asn Ala Ile Val Val Leu Glu Asp Leu Asn Phe Gly Phe Lys Gln 
    930                 935                 940                 


Gly Arg Gln Lys Phe Glu Arg Gln Val Tyr Gln Lys Phe Glu Lys Met 
945                 950                 955                 960 


Leu Ile Asp Lys Leu Asn Tyr Leu Val Asp Lys Ser Lys Gly Met Asp 
                965                 970                 975     


Glu Asp Gly Gly Leu Leu His Ala Tyr Gln Leu Thr Asp Glu Phe Lys 
            980                 985                 990         


Ser Phe Lys Gln Leu Gly Lys Gln  Ser Gly Phe Leu Tyr  Tyr Ile Pro 
        995                 1000                 1005             


Ala Trp  Asn Thr Ser Lys Leu  Asp Pro Thr Thr Gly  Phe Val Asn 
    1010                 1015                 1020             


Leu Phe  Tyr Thr Lys Tyr Glu  Ser Val Glu Lys Ser  Lys Glu Phe 
    1025                 1030                 1035             


Ile Asn  Asn Phe Thr Ser Ile  Leu Tyr Asn Gln Glu  Arg Glu Tyr 
    1040                 1045                 1050             


Phe Glu  Phe Leu Phe Asp Tyr  Ser Ala Phe Thr Ser  Lys Ala Glu 
    1055                 1060                 1065             


Gly Ser  Arg Leu Lys Trp Thr  Val Cys Ser Lys Gly  Glu Arg Val 
    1070                 1075                 1080             


Glu Thr  Tyr Arg Asn Pro Lys  Lys Asn Asn Glu Trp  Asp Thr Gln 
    1085                 1090                 1095             


Lys Ile  Asp Leu Thr Phe Glu  Leu Lys Lys Leu Phe  Asn Asp Tyr 
    1100                 1105                 1110             


Ser Ile  Ser Leu Leu Asp Gly  Asp Leu Arg Glu Gln  Met Gly Lys 
    1115                 1120                 1125             


Ile Asp  Lys Ala Asp Phe Tyr  Lys Lys Phe Met Lys  Leu Phe Ala 
    1130                 1135                 1140             


Leu Ile  Val Gln Met Arg Asn  Ser Asp Glu Arg Glu  Asp Lys Leu 
    1145                 1150                 1155             


Ile Ser  Pro Val Leu Asn Lys  Tyr Gly Ala Phe Phe  Glu Thr Gly 
    1160                 1165                 1170             


Lys Asn  Glu Arg Met Pro Leu  Asp Ala Asp Ala Asn  Gly Ala Tyr 
    1175                 1180                 1185             


Asn Ile  Ala Arg Lys Gly Leu  Trp Ile Ile Glu Lys  Ile Lys Asn 
    1190                 1195                 1200             


Thr Asp  Val Glu Gln Leu Asp  Lys Val Lys Leu Thr  Ile Ser Asn 
    1205                 1210                 1215             


Lys Glu  Trp Leu Gln Tyr Ala  Gln Glu His Ile Leu  
    1220                 1225                 1230 


<210>  26
<211>  1307
<212>  PRT
<213>  Succiniclasticum ruminis

<400>  26

Met Gly Asn Phe Gly Glu Phe Thr His Lys Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Val Ala 
            20                  25                  30          


Lys Tyr Gly Ile Val Asp Asp Asp Lys Arg Arg Ser Glu Asn Tyr Lys 
        35                  40                  45              


Lys Leu Lys Pro Val Ile Asp Arg Ile Tyr Lys Tyr Phe Ile Asp Glu 
    50                  55                  60                  


Ser Leu Lys Asn Val Ser Ile Asp Trp Gln Pro Leu Tyr Glu Ala Ile 
65                  70                  75                  80  


Ile Ala Tyr Arg Lys Glu Gln Thr Thr Ala Asn Val Val Arg Leu Lys 
                85                  90                  95      


Glu Glu Gln Glu Ala Cys Arg Lys Ala Ile Ala Ala Trp Phe Glu Gly 
            100                 105                 110         


Lys Val Pro Asp Lys Gly Ser Lys Asp Leu Lys Glu Phe Asn Lys Thr 
        115                 120                 125             


Gln Ser Lys Leu Phe Lys Glu Leu Phe Gly Lys Glu Leu Phe Thr Glu 
    130                 135                 140                 


Ser Val Thr Gln Leu Leu Pro Gly Leu Ser Leu Thr Glu Glu Glu Lys 
145                 150                 155                 160 


Glu Leu Leu Ala Ser Phe Asn Lys Phe Thr Ser Tyr Phe Lys Gly Phe 
                165                 170                 175     


Tyr Val Asn Arg Lys Asn Val Phe Ser Ala Asp Asp Ile Ser Thr Ser 
            180                 185                 190         


Ile Pro His Arg Leu Val Gln Glu Asn Phe Pro Lys Phe Met Asp Asn 
        195                 200                 205             


Cys Glu Ala Tyr Arg Arg Ile Val Glu Glu Tyr Pro Glu Leu Lys Ala 
    210                 215                 220                 


Lys Leu Glu Gly Thr Ala Gln Ala Thr Gly Ile Phe Ile Gly Phe Lys 
225                 230                 235                 240 


Leu Asp Asn Ile Phe Lys Val Ser Phe Tyr Asn His Leu Leu Gln Gln 
                245                 250                 255     


Ser Gln Ile Asp Leu Tyr Asn Gln Phe Leu Cys Gly Ile Ala Gly Glu 
            260                 265                 270         


Glu Gly Thr Met Arg Val Gln Gly Leu Asn Val Thr Leu Asn Leu Ala 
        275                 280                 285             


Met Lys Gln Asp Lys Val Leu Gly Gln Lys Leu Lys Ser Met Pro His 
    290                 295                 300                 


Arg Phe Ile Pro Leu Tyr Lys Gln Ile Leu Ser Asp Arg Thr Thr Leu 
305                 310                 315                 320 


Ser Phe Ile Pro Glu Ala Phe Gln Asn Asp Glu Glu Val Leu Leu Thr 
                325                 330                 335     


Val Glu Glu Tyr Arg Lys Ser Leu Glu Ala Glu Arg Thr Thr Gly Ala 
            340                 345                 350         


Val Ser Asp Ile Phe Asn Ser Leu Gln Ala Ala Asp Leu Arg His Val 
        355                 360                 365             


Tyr Val Asn Pro Ala Lys Leu Thr Ala Phe Ser Gln Met Leu Phe Glu 
    370                 375                 380                 


Asp Trp Ser Leu Cys Arg Glu Ser Leu Arg Asn Trp Lys Leu Arg Ser 
385                 390                 395                 400 


Tyr Gly Lys Ala Ala Thr Lys Lys Val Arg Glu Glu Ile Glu Ser Trp 
                405                 410                 415     


Leu Lys Glu Ser Ala Ile Ser Leu Asp Glu Leu Gln Ala Ala Leu Ala 
            420                 425                 430         


Asp Gly Thr Leu Ser Val Ile Ile Asn Gln Lys Val Gln Ser Val Ile 
        435                 440                 445             


Thr Thr Leu Glu Gln Glu Leu Ala Lys Pro Leu Pro Lys Lys Leu Lys 
    450                 455                 460                 


Thr Ala Glu Glu Lys Glu Ser Leu Lys Ser Leu Leu Asp Ser Val Gln 
465                 470                 475                 480 


Glu Ala Cys His Ser Leu Glu Met Phe Ala Val Gly Glu Asn Met Asp 
                485                 490                 495     


Thr Asp Pro Cys Phe Tyr Val Pro Leu Arg Glu Ala Met Glu Ala Ile 
            500                 505                 510         


Gln Pro Ile Ile Pro Leu Tyr Asn Lys Val Arg Asn Phe Ala Thr Gln 
        515                 520                 525             


Lys Pro Tyr Ser Ile Glu Lys Phe Lys Leu Asn Phe Ser Asn Pro Ile 
    530                 535                 540                 


Leu Ala Ser Gly Trp Asp Glu Asn Arg Glu Arg Gln Thr Cys Ala Ile 
545                 550                 555                 560 


Leu Phe Arg Lys Gly Glu Lys Tyr Tyr Leu Gly Ile Tyr Asn Ala Lys 
                565                 570                 575     


Val Lys Pro Asp Phe Ser Ile Ile Lys Ala Val Lys Gly Gly Asn Cys 
            580                 585                 590         


Phe Glu Lys Val Val Tyr Arg Gln Phe Pro Asp Phe Ser Lys Met Met 
        595                 600                 605             


Pro Lys Cys Thr Thr Gln Leu Lys Glu Val Gln Gln His Phe Ala Ser 
    610                 615                 620                 


Ser Ser Glu Asp Tyr Val Leu Tyr Asn Lys Lys Phe Ile Lys Pro Leu 
625                 630                 635                 640 


Thr Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Val Leu Phe Asp Gly 
                645                 650                 655     


Lys Lys Lys Phe Gln Ile Asp Tyr Leu Arg Lys Thr Lys Asp Glu Asp 
            660                 665                 670         


Gly Tyr Tyr His Ala Leu His Thr Trp Ile Asn Phe Ala Lys Glu Phe 
        675                 680                 685             


Val Ala Ser Tyr Glu Ser Thr Ser Ile Tyr Asp Thr Ser Thr Val Leu 
    690                 695                 700                 


Ser Thr Glu Gln Tyr Val Lys Leu Asn Asp Phe Tyr Gly Asp Leu Asp 
705                 710                 715                 720 


Asn Leu Phe Tyr Arg Ile Lys Phe Glu Ser Val Ser Glu Glu Thr Ile 
                725                 730                 735     


Ser Glu Phe Val Asp Glu Gly Lys Leu Phe Leu Phe Gln Ile Tyr Asn 
            740                 745                 750         


Lys Asp Phe Ala Glu Gly Ala Thr Gly Ala Pro Asn Leu His Thr Ile 
        755                 760                 765             


Tyr Trp Lys Ala Val Phe Asp Pro Glu Asn Met Lys Asn Val Val Val 
    770                 775                 780                 


Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys Ser Ala Met 
785                 790                 795                 800 


Asp Ile Val Arg His Lys Val Gly Glu Lys Leu Val Asn Arg Arg Leu 
                805                 810                 815     


Lys Asp Gly Thr Ser Leu Thr Glu Glu Leu His Glu Glu Leu Tyr Leu 
            820                 825                 830         


Tyr Ala Asn Gly Lys Leu Lys Lys Lys Leu Ser Glu Ala Ala Ala Ala 
        835                 840                 845             


Val Leu Pro Gln Ala Val Ile Tyr Asp Val His His Glu Ile Val Lys 
    850                 855                 860                 


Asp Arg Arg Phe Thr Glu Asp Lys Phe Phe Phe His Val Pro Leu Thr 
865                 870                 875                 880 


Leu Asn Tyr Lys Cys Asp Lys Asn Ala Val Gln Phe Asn Ala Ser Val 
                885                 890                 895     


Gln Glu Tyr Leu Lys Glu Asn Pro Asp Thr Tyr Ile Ile Gly Ile Asp 
            900                 905                 910         


Arg Gly Glu Arg Asn Leu Ile Tyr Ala Val Val Ile Asp Pro Gln Gly 
        915                 920                 925             


Asn Ile Val Glu Gln Lys Ser Phe Asn Val Ile Asn Gly Phe Asp Tyr 
    930                 935                 940                 


His Asn Lys Leu Glu Gln Arg Glu Lys Glu Arg Asn Lys Ala Arg Gln 
945                 950                 955                 960 


Asp Trp Thr Thr Val Gly Lys Ile Lys Glu Leu Lys Gln Gly Tyr Leu 
                965                 970                 975     


Ser Leu Val Val His Glu Ile Thr Ser Met Met Val Lys Tyr Asn Ala 
            980                 985                 990         


Ile Val Val Leu Glu Asn Leu Asn  Val Gly Phe Lys Arg  Ile Arg Ser 
        995                 1000                 1005             


Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln Phe Glu  Lys Met Leu 
    1010                 1015                 1020             


Ile Asn  Lys Leu Asn Tyr Leu  Met Phe Lys Asp Val  Glu Gly Ala 
    1025                 1030                 1035             


Lys Pro  Gly Ser Val Leu Asn  Ala Tyr Gln Leu Thr  Asp Arg Phe 
    1040                 1045                 1050             


Glu Ser  Phe Ala Ser Met Arg  Asn Gln Thr Gly Phe  Leu Phe Tyr 
    1055                 1060                 1065             


Ile Pro  Ala Ala Phe Thr Ser  Lys Ile Asp Pro Ala  Thr Gly Phe 
    1070                 1075                 1080             


Val Asp  Pro Phe Cys Trp Ser  Ala Ile Lys Thr Leu  Asp Asp Lys 
    1085                 1090                 1095             


Lys Thr  Phe Ile Ser Gly Phe  Asp Thr Leu Lys Tyr  Asp Asn Val 
    1100                 1105                 1110             


Thr Gly  Asn Phe Ile Leu His  Phe Glu Met Lys Lys  Asn Lys Asp 
    1115                 1120                 1125             


Phe Gln  Lys Lys Leu Glu Gly  Phe Met Pro Glu Trp  Asp Ile Val 
    1130                 1135                 1140             


Val Glu  Ala Asn Lys Asp Arg  Arg Asp Ala Glu Gly  Lys Thr Phe 
    1145                 1150                 1155             


Ile Ser  Gly Lys Arg Ile Glu  Phe Val Arg Glu Asn  Asn Gly His 
    1160                 1165                 1170             


Gly His  Tyr Glu Asp Tyr Leu  Pro Cys Lys Lys Leu  Val Glu Ile 
    1175                 1180                 1185             


Leu Arg  Gln Tyr Asp Ile Leu  Phe Glu Asp Gly Lys  Asp Val Leu 
    1190                 1195                 1200             


Pro Leu  Ile Met Lys Asn Gly  Asp Ser Lys Leu Ile  His Glu Val 
    1205                 1210                 1215             


Phe Lys  Val Ile Arg Leu Ser  Leu Gln Met Arg Asn  Ser Asn Ala 
    1220                 1225                 1230             


Glu Ser  Gly Glu Asp Phe Ile  Ser Ser Pro Val Glu  Asn Asn Glu 
    1235                 1240                 1245             


Gly Ile  Cys Phe Asp Ser Arg  Leu Gly Val Glu Thr  Leu Pro Lys 
    1250                 1255                 1260             


Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile Ala Leu  Lys Gly Leu 
    1265                 1270                 1275             


Leu Leu  Leu Glu Lys Ile Arg  His Asp Glu Arg Lys  Leu Gly Ile 
    1280                 1285                 1290             


Ser Asn  Ser Glu Trp Leu Asn  His Ile Gln Ser Leu  Arg Gly 
    1295                 1300                 1305         


<210>  27
<211>  1231
<212>  PRT
<213>  Butyrivibrio fibrisolvens

<400>  27

Met Tyr Tyr Glu Ser Leu Thr Lys Leu Tyr Pro Ile Lys Lys Thr Ile 
1               5                   10                  15      


Arg Asn Glu Leu Val Pro Ile Gly Lys Thr Leu Glu Asn Ile Lys Lys 
            20                  25                  30          


Asn Asn Ile Leu Glu Ala Asp Glu Asp Arg Lys Ile Ala Tyr Ile Arg 
        35                  40                  45              


Val Lys Ala Ile Met Asp Asp Tyr His Lys Arg Leu Ile Asn Glu Ala 
    50                  55                  60                  


Leu Ser Gly Phe Ala Leu Ile Asp Leu Asp Lys Ala Ala Asn Leu Tyr 
65                  70                  75                  80  


Leu Ser Arg Ser Lys Ser Ala Asp Asp Ile Glu Ser Phe Ser Arg Phe 
                85                  90                  95      


Gln Asp Lys Leu Arg Lys Ala Ile Ala Lys Arg Leu Arg Glu His Glu 
            100                 105                 110         


Asn Phe Gly Lys Ile Gly Asn Lys Asp Ile Ile Pro Leu Leu Gln Lys 
        115                 120                 125             


Leu Ser Glu Asn Glu Asp Asp Tyr Asn Ala Leu Glu Ser Phe Lys Asn 
    130                 135                 140                 


Phe Tyr Thr Tyr Phe Glu Ser Tyr Asn Asp Val Arg Leu Asn Leu Tyr 
145                 150                 155                 160 


Ser Asp Lys Glu Lys Ser Ser Thr Val Ala Tyr Arg Leu Ile Asn Glu 
                165                 170                 175     


Asn Leu Pro Arg Phe Leu Asp Asn Ile Arg Ala Tyr Asp Ala Val Gln 
            180                 185                 190         


Lys Ala Gly Ile Thr Ser Glu Glu Leu Ser Ser Glu Ala Gln Asp Gly 
        195                 200                 205             


Leu Phe Leu Val Asn Thr Phe Asn Asn Val Leu Ile Gln Asp Gly Ile 
    210                 215                 220                 


Asn Thr Tyr Asn Glu Asp Ile Gly Lys Leu Asn Val Ala Ile Asn Leu 
225                 230                 235                 240 


Tyr Asn Gln Lys Asn Ala Ser Val Gln Gly Phe Arg Lys Val Pro Lys 
                245                 250                 255     


Met Lys Val Leu Tyr Lys Gln Ile Leu Ser Asp Arg Glu Glu Ser Phe 
            260                 265                 270         


Ile Asp Glu Phe Glu Ser Asp Thr Glu Leu Leu Asp Ser Leu Glu Ser 
        275                 280                 285             


His Tyr Ala Asn Leu Ala Lys Tyr Phe Gly Ser Asn Lys Val Gln Leu 
    290                 295                 300                 


Leu Phe Thr Ala Leu Arg Glu Ser Lys Gly Val Asn Val Tyr Val Lys 
305                 310                 315                 320 


Asn Asp Ile Ala Lys Thr Ser Phe Ser Asn Val Val Phe Gly Ser Trp 
                325                 330                 335     


Ser Arg Ile Asp Glu Leu Ile Asn Gly Glu Tyr Asp Asp Asn Asn Asn 
            340                 345                 350         


Arg Lys Lys Asp Glu Lys Tyr Tyr Asp Lys Arg Gln Lys Glu Leu Lys 
        355                 360                 365             


Lys Asn Lys Ser Tyr Thr Ile Glu Lys Ile Ile Thr Leu Ser Thr Glu 
    370                 375                 380                 


Asp Val Asp Val Ile Gly Lys Tyr Ile Glu Lys Leu Glu Ser Asp Ile 
385                 390                 395                 400 


Asp Asp Ile Arg Phe Lys Gly Lys Asn Phe Tyr Glu Ala Val Leu Cys 
                405                 410                 415     


Gly His Asp Arg Ser Lys Lys Leu Ser Lys Asn Lys Gly Ala Val Glu 
            420                 425                 430         


Ala Ile Lys Gly Tyr Leu Asp Ser Val Lys Asp Phe Glu Arg Asp Leu 
        435                 440                 445             


Lys Leu Ile Asn Gly Ser Gly Gln Glu Leu Glu Lys Asn Leu Val Val 
    450                 455                 460                 


Tyr Gly Glu Gln Glu Ala Val Leu Ser Glu Leu Ser Gly Ile Asp Ser 
465                 470                 475                 480 


Leu Tyr Asn Met Thr Arg Asn Tyr Leu Thr Lys Lys Pro Phe Ser Thr 
                485                 490                 495     


Glu Lys Ile Lys Leu Asn Phe Asn Lys Pro Thr Phe Leu Asp Gly Trp 
            500                 505                 510         


Asp Tyr Gly Asn Glu Glu Ala Tyr Leu Gly Phe Phe Met Ile Lys Glu 
        515                 520                 525             


Gly Asn Tyr Phe Leu Ala Val Met Asp Ala Asn Trp Asn Lys Glu Phe 
    530                 535                 540                 


Arg Asn Ile Pro Ser Val Asp Lys Ser Asp Cys Tyr Lys Lys Val Ile 
545                 550                 555                 560 


Tyr Lys Gln Ile Ser Ser Pro Glu Lys Ser Ile Gln Asn Leu Met Val 
                565                 570                 575     


Ile Asp Gly Lys Thr Val Lys Lys Asn Gly Arg Lys Glu Lys Glu Gly 
            580                 585                 590         


Ile His Ser Gly Glu Asn Leu Ile Leu Glu Glu Leu Lys Asn Thr Tyr 
        595                 600                 605             


Leu Pro Lys Lys Ile Asn Asp Ile Arg Lys Arg Arg Ser Tyr Leu Asn 
    610                 615                 620                 


Gly Asp Thr Phe Ser Lys Lys Asp Leu Thr Glu Phe Ile Gly Tyr Tyr 
625                 630                 635                 640 


Lys Gln Arg Val Ile Glu Tyr Tyr Asn Gly Tyr Ser Phe Tyr Phe Lys 
                645                 650                 655     


Ser Asp Asp Asp Tyr Ala Ser Phe Lys Glu Phe Gln Glu Asp Val Gly 
            660                 665                 670         


Arg Gln Ala Tyr Gln Ile Ser Tyr Val Asp Val Pro Val Ser Phe Val 
        675                 680                 685             


Asp Asp Leu Ile Asn Ser Gly Lys Leu Tyr Leu Phe Arg Val Tyr Asn 
    690                 695                 700                 


Lys Asp Phe Ser Glu Tyr Ser Lys Gly Arg Leu Asn Leu His Thr Leu 
705                 710                 715                 720 


Tyr Phe Lys Met Leu Phe Asp Glu Arg Asn Leu Lys Asn Val Val Tyr 
                725                 730                 735     


Lys Leu Asn Gly Gln Ala Glu Val Phe Tyr Arg Pro Ser Ser Ile Lys 
            740                 745                 750         


Lys Glu Glu Leu Ile Val His Arg Ala Gly Glu Glu Ile Lys Asn Lys 
        755                 760                 765             


Asn Pro Lys Arg Ala Ala Gln Lys Pro Thr Arg Arg Leu Asp Tyr Asp 
    770                 775                 780                 


Ile Val Lys Asp Arg Arg Tyr Ser Gln Asp Lys Phe Met Leu His Thr 
785                 790                 795                 800 


Ser Ile Ile Met Asn Phe Gly Ala Glu Glu Asn Val Ser Phe Asn Asp 
                805                 810                 815     


Ile Val Asn Gly Val Leu Arg Asn Glu Asp Lys Val Asn Val Ile Gly 
            820                 825                 830         


Ile Asp Arg Gly Glu Arg Asn Leu Leu Tyr Val Val Val Ile Asp Pro 
        835                 840                 845             


Glu Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Cys Ile Thr Asp Ser 
    850                 855                 860                 


Asn Leu Asp Ile Glu Thr Asp Tyr His Arg Leu Leu Asp Glu Lys Glu 
865                 870                 875                 880 


Ser Asp Arg Lys Ile Ala Arg Arg Asp Trp Thr Thr Ile Glu Asn Ile 
                885                 890                 895     


Lys Glu Leu Lys Ala Gly Tyr Leu Ser Gln Val Val His Ile Val Ala 
            900                 905                 910         


Glu Leu Val Leu Lys Tyr Asn Ala Ile Ile Cys Leu Glu Asp Leu Asn 
        915                 920                 925             


Phe Gly Phe Lys Arg Gly Arg Gln Lys Val Glu Lys Gln Val Tyr Gln 
    930                 935                 940                 


Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Leu Val Met Asp 
945                 950                 955                 960 


Lys Ser Arg Glu Gln Leu Ser Pro Glu Lys Ile Ser Gly Ala Leu Asn 
                965                 970                 975     


Ala Leu Gln Leu Thr Pro Asp Phe Lys Ser Phe Lys Val Leu Gly Lys 
            980                 985                 990         


Gln Thr Gly Ile Ile Tyr Tyr Val  Pro Ala Tyr Leu Thr  Ser Lys Ile 
        995                 1000                 1005             


Asp Pro  Met Thr Gly Phe Ala  Asn Leu Phe Tyr Val  Lys Tyr Glu 
    1010                 1015                 1020             


Asn Val  Asp Lys Ala Lys Glu  Phe Phe Ser Lys Phe  Asp Ser Ile 
    1025                 1030                 1035             


Lys Tyr  Asn Lys Asp Gly Lys  Asn Trp Asn Thr Lys  Gly Tyr Phe 
    1040                 1045                 1050             


Glu Phe  Ala Phe Asp Tyr Lys  Lys Phe Thr Asp Arg  Ala Tyr Gly 
    1055                 1060                 1065             


Arg Val  Ser Glu Trp Thr Val  Cys Thr Val Gly Glu  Arg Ile Ile 
    1070                 1075                 1080             


Lys Phe  Lys Asn Lys Glu Lys  Asn Asn Ser Tyr Asp  Asp Lys Val 
    1085                 1090                 1095             


Ile Asp  Leu Thr Asn Ser Leu  Lys Glu Leu Phe Asp  Ser Tyr Lys 
    1100                 1105                 1110             


Val Thr  Tyr Glu Ser Glu Val  Asp Leu Lys Asp Ala  Ile Leu Ala 
    1115                 1120                 1125             


Ile Asp  Asp Pro Ala Phe Tyr  Arg Asp Leu Thr Arg  Arg Leu Gln 
    1130                 1135                 1140             


Gln Thr  Leu Gln Met Arg Asn  Ser Ser Cys Asp Gly  Ser Arg Asp 
    1145                 1150                 1155             


Tyr Ile  Ile Ser Pro Val Lys  Asn Ser Lys Gly Glu  Phe Phe Cys 
    1160                 1165                 1170             


Ser Asp  Asn Asn Asp Asp Thr  Thr Pro Asn Asp Ala  Asp Ala Asn 
    1175                 1180                 1185             


Gly Ala  Phe Asn Ile Ala Arg  Lys Gly Leu Trp Val  Leu Asn Glu 
    1190                 1195                 1200             


Ile Arg  Asn Ser Glu Glu Gly  Ser Lys Ile Asn Leu  Ala Met Ser 
    1205                 1210                 1215             


Asn Ala  Gln Trp Leu Glu Tyr  Ala Gln Asp Asn Thr  Ile 
    1220                 1225                 1230     


<210>  28
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(37)
<223>  non-specific ssDNA

<400>  28
agcatgccca aattgcttac atatgtgtta cgacggt                                37


<210>  29
<211>  3900
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3900)
<223>  FnCpf1 Q1025G mutant

<400>  29
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat     3000

gctattgtgg tttttgagga tttaaatttt ggatttaaaa gagggcgttt caaggtagag     3060

aagcaggtct atggaaagtt agaaaaaatg ctaattgaga aactaaacta tctagttttc     3120

aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct     3180

tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt     3240

tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa     3300

agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat     3360

aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc     3420

aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat     3480

cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat     3540

tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac     3600

aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca     3660

aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc     3720

tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat     3780

catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa     3840

aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac     3900


<210>  30
<211>  1287
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1287)
<223>  Q1025E Cpf1 mutant

<400>  30

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val 
                325                 330                 335     


Thr Thr Met Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val 
            340                 345                 350         


Glu Glu Lys Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu 
        355                 360                 365             


Lys Ala Gln Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys 
    370                 375                 380                 


Ser Leu Thr Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile 
385                 390                 395                 400 


Gly Thr Ala Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn 
                405                 410                 415     


Leu Asp Asn Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr 
            420                 425                 430         


Glu Lys Ala Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu 
        435                 440                 445             


Glu Phe Asn Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu 
    450                 455                 460                 


Ile Leu Ala Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala 
465                 470                 475                 480 


Gln Asn Lys Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln 
                485                 490                 495     


Gly Lys Lys Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala 
            500                 505                 510         


Ile Lys Asp Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys 
        515                 520                 525             


Ile Phe His Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys 
    530                 535                 540                 


Asp Glu His Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala 
545                 550                 555                 560 


Asn Ile Val Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys 
                565                 570                 575     


Pro Tyr Ser Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu 
            580                 585                 590         


Ala Asn Gly Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu 
        595                 600                 605             


Phe Ile Lys Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn 
    610                 615                 620                 


Asn Lys Ile Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly 
625                 630                 635                 640 


Tyr Lys Lys Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu 
                645                 650                 655     


Pro Lys Val Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser 
            660                 665                 670         


Glu Asp Ile Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly 
        675                 680                 685             


Ser Pro Gln Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys 
    690                 695                 700                 


Arg Lys Phe Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu 
705                 710                 715                 720 


Trp Lys Asp Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser 
                725                 730                 735     


Ile Asp Glu Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr 
            740                 745                 750         


Phe Glu Asn Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly 
        755                 760                 765             


Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser 
    770                 775                 780                 


Lys Gly Arg Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp 
785                 790                 795                 800 


Glu Arg Asn Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu 
                805                 810                 815     


Leu Phe Tyr Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala 
            820                 825                 830         


Lys Glu Ala Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser 
        835                 840                 845             


Val Phe Glu Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys 
    850                 855                 860                 


Phe Phe Phe His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala 
865                 870                 875                 880 


Asn Lys Phe Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn 
                885                 890                 895     


Asp Val His Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr 
            900                 905                 910         


Tyr Thr Leu Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe 
        915                 920                 925             


Asn Ile Ile Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu 
    930                 935                 940                 


Ala Ala Ile Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys 
945                 950                 955                 960 


Ile Asn Asn Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val 
                965                 970                 975     


His Glu Ile Ala Lys Leu Val Ile Glu Tyr Asn Ala Ile Val Val Phe 
            980                 985                 990         


Glu Asp Leu Asn Phe Gly Phe Lys  Arg Gly Arg Phe Lys  Val Glu Lys 
        995                 1000                 1005             


Gln Val  Tyr Gly Lys Leu Glu  Lys Met Leu Ile Glu  Lys Leu Asn 
    1010                 1015                 1020             


Tyr Leu  Val Phe Lys Asp Asn  Glu Phe Asp Lys Thr  Gly Gly Val 
    1025                 1030                 1035             


Leu Arg  Ala Tyr Gln Leu Thr  Ala Pro Phe Glu Thr  Phe Lys Lys 
    1040                 1045                 1050             


Met Gly  Lys Gln Thr Gly Ile  Ile Tyr Tyr Val Pro  Ala Gly Phe 
    1055                 1060                 1065             


Thr Ser  Lys Ile Cys Pro Val  Thr Gly Phe Val Asn  Gln Leu Tyr 
    1070                 1075                 1080             


Pro Lys  Tyr Glu Ser Val Ser  Lys Ser Gln Glu Phe  Phe Ser Lys 
    1085                 1090                 1095             


Phe Asp  Lys Ile Cys Tyr Asn  Leu Asp Lys Gly Tyr  Phe Glu Phe 
    1100                 1105                 1110             


Ser Phe  Asp Tyr Lys Asn Phe  Gly Asp Lys Ala Ala  Lys Gly Lys 
    1115                 1120                 1125             


Trp Thr  Ile Ala Ser Phe Gly  Ser Arg Leu Ile Asn  Phe Arg Asn 
    1130                 1135                 1140             


Ser Asp  Lys Asn His Asn Trp  Asp Thr Arg Glu Val  Tyr Pro Thr 
    1145                 1150                 1155             


Lys Glu  Leu Glu Lys Leu Leu  Lys Asp Tyr Ser Ile  Glu Tyr Gly 
    1160                 1165                 1170             


His Gly  Glu Cys Ile Lys Ala  Ala Ile Cys Gly Glu  Ser Asp Lys 
    1175                 1180                 1185             


Lys Phe  Phe Ala Lys Leu Thr  Ser Val Leu Asn Thr  Ile Leu Gln 
    1190                 1195                 1200             


Met Arg  Asn Ser Lys Thr Gly  Thr Glu Leu Asp Tyr  Leu Ile Ser 
    1205                 1210                 1215             


Pro Val  Ala Asp Val Asn Gly  Asn Phe Phe Asp Ser  Arg Gln Ala 
    1220                 1225                 1230             


Pro Lys  Asn Met Pro Gln Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1235                 1240                 1245             


Ile Gly  Leu Lys Gly Leu Met  Leu Leu Gly Arg Ile  Lys Asn Asn 
    1250                 1255                 1260             


Gln Glu  Gly Lys Lys Leu Asn  Leu Val Ile Lys Asn  Glu Glu Tyr 
    1265                 1270                 1275             


Phe Glu  Phe Val Gln Asn Arg  Asn Asn 
    1280                 1285         


<210>  31
<211>  3900
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3900)
<223>  Q1025G E1028G Cpf1 mutant

<400>  31
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat     3000

gctattgtgg tttttgagga tttaaatttt ggatttaaaa gagggcgttt caaggtagag     3060

aagcaggtct atggaaagtt aggaaaaatg ctaattgaga aactaaacta tctagttttc     3120

aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct     3180

tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt     3240

tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa     3300

agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat     3360

aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc     3420

aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat     3480

cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat     3540

tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac     3600

aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca     3660

aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc     3720

tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat     3780

catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa     3840

aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac     3900


<210>  32
<211>  1287
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1287)
<223>  Q1025G E1028G mutant

<400>  32

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val 
                325                 330                 335     


Thr Thr Met Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val 
            340                 345                 350         


Glu Glu Lys Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu 
        355                 360                 365             


Lys Ala Gln Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys 
    370                 375                 380                 


Ser Leu Thr Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile 
385                 390                 395                 400 


Gly Thr Ala Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn 
                405                 410                 415     


Leu Asp Asn Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr 
            420                 425                 430         


Glu Lys Ala Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu 
        435                 440                 445             


Glu Phe Asn Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu 
    450                 455                 460                 


Ile Leu Ala Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala 
465                 470                 475                 480 


Gln Asn Lys Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln 
                485                 490                 495     


Gly Lys Lys Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala 
            500                 505                 510         


Ile Lys Asp Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys 
        515                 520                 525             


Ile Phe His Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys 
    530                 535                 540                 


Asp Glu His Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala 
545                 550                 555                 560 


Asn Ile Val Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys 
                565                 570                 575     


Pro Tyr Ser Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu 
            580                 585                 590         


Ala Asn Gly Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu 
        595                 600                 605             


Phe Ile Lys Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn 
    610                 615                 620                 


Asn Lys Ile Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly 
625                 630                 635                 640 


Tyr Lys Lys Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu 
                645                 650                 655     


Pro Lys Val Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser 
            660                 665                 670         


Glu Asp Ile Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly 
        675                 680                 685             


Ser Pro Gln Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys 
    690                 695                 700                 


Arg Lys Phe Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu 
705                 710                 715                 720 


Trp Lys Asp Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser 
                725                 730                 735     


Ile Asp Glu Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr 
            740                 745                 750         


Phe Glu Asn Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly 
        755                 760                 765             


Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser 
    770                 775                 780                 


Lys Gly Arg Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp 
785                 790                 795                 800 


Glu Arg Asn Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu 
                805                 810                 815     


Leu Phe Tyr Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala 
            820                 825                 830         


Lys Glu Ala Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser 
        835                 840                 845             


Val Phe Glu Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys 
    850                 855                 860                 


Phe Phe Phe His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala 
865                 870                 875                 880 


Asn Lys Phe Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn 
                885                 890                 895     


Asp Val His Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr 
            900                 905                 910         


Tyr Thr Leu Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe 
        915                 920                 925             


Asn Ile Ile Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu 
    930                 935                 940                 


Ala Ala Ile Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys 
945                 950                 955                 960 


Ile Asn Asn Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val 
                965                 970                 975     


His Glu Ile Ala Lys Leu Val Ile Glu Tyr Asn Ala Ile Val Val Phe 
            980                 985                 990         


Glu Asp Leu Asn Phe Gly Phe Lys  Arg Gly Arg Phe Lys  Val Glu Lys 
        995                 1000                 1005             


Gln Val  Tyr Gly Lys Leu Gly  Lys Met Leu Ile Glu  Lys Leu Asn 
    1010                 1015                 1020             


Tyr Leu  Val Phe Lys Asp Asn  Glu Phe Asp Lys Thr  Gly Gly Val 
    1025                 1030                 1035             


Leu Arg  Ala Tyr Gln Leu Thr  Ala Pro Phe Glu Thr  Phe Lys Lys 
    1040                 1045                 1050             


Met Gly  Lys Gln Thr Gly Ile  Ile Tyr Tyr Val Pro  Ala Gly Phe 
    1055                 1060                 1065             


Thr Ser  Lys Ile Cys Pro Val  Thr Gly Phe Val Asn  Gln Leu Tyr 
    1070                 1075                 1080             


Pro Lys  Tyr Glu Ser Val Ser  Lys Ser Gln Glu Phe  Phe Ser Lys 
    1085                 1090                 1095             


Phe Asp  Lys Ile Cys Tyr Asn  Leu Asp Lys Gly Tyr  Phe Glu Phe 
    1100                 1105                 1110             


Ser Phe  Asp Tyr Lys Asn Phe  Gly Asp Lys Ala Ala  Lys Gly Lys 
    1115                 1120                 1125             


Trp Thr  Ile Ala Ser Phe Gly  Ser Arg Leu Ile Asn  Phe Arg Asn 
    1130                 1135                 1140             


Ser Asp  Lys Asn His Asn Trp  Asp Thr Arg Glu Val  Tyr Pro Thr 
    1145                 1150                 1155             


Lys Glu  Leu Glu Lys Leu Leu  Lys Asp Tyr Ser Ile  Glu Tyr Gly 
    1160                 1165                 1170             


His Gly  Glu Cys Ile Lys Ala  Ala Ile Cys Gly Glu  Ser Asp Lys 
    1175                 1180                 1185             


Lys Phe  Phe Ala Lys Leu Thr  Ser Val Leu Asn Thr  Ile Leu Gln 
    1190                 1195                 1200             


Met Arg  Asn Ser Lys Thr Gly  Thr Glu Leu Asp Tyr  Leu Ile Ser 
    1205                 1210                 1215             


Pro Val  Ala Asp Val Asn Gly  Asn Phe Phe Asp Ser  Arg Gln Ala 
    1220                 1225                 1230             


Pro Lys  Asn Met Pro Gln Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1235                 1240                 1245             


Ile Gly  Leu Lys Gly Leu Met  Leu Leu Gly Arg Ile  Lys Asn Asn 
    1250                 1255                 1260             


Gln Glu  Gly Lys Lys Leu Asn  Leu Val Ile Lys Asn  Glu Glu Tyr 
    1265                 1270                 1275             


Phe Glu  Phe Val Gln Asn Arg  Asn Asn 
    1280                 1285         


<210>  33
<211>  3861
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3861)
<223>  Rec-deletion Cpf1 mutant

<400>  33
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt ttgtaattga taagttagaa gatgatagtg atgtagttac aacgatgcaa     1020

agtttttatg agcaaatagc agcttttaaa acagtagaag aaaaatctat taaagaaaca     1080

ctatctttat tatttgatga tttaaaagct caaaaacttg atttgagtaa aatttatttt     1140

aaaaatgata aatctcttac tgatctatca caacaagttt ttgatgatta tagtgttatt     1200

ggtacagcgg tactagaata tataactcaa caaatagcac ctaaaaatct tgataaccct     1260

agtaagaaag agcaagaatt aatagccaaa aaaactgaaa aagcaaaata cttatctcta     1320

gaaactataa agcttgcctt agaagaattt aataagcata gagatataga taaacagtgt     1380

aggtttgaag aaatacttgc aaactttgcg gctattccga tgatatttga tgaaatagct     1440

caaaacaaag acaatttggc acagatatct atcaaatatc aaaatcaagg taaaaaagac     1500

ctacttcaag ctagtgcgga agatgatgtt aaagctatca aggatctttt agatcaaact     1560

aataatctct tacataaact aaaaatattt catattagtc agtcagaaga taaggcaaat     1620

attttagaca aggatgagca tttttatcta gtatttgagg agtgctactt tgagctagcg     1680

aatatagtgc ctctttataa caaaattaga aactatataa ctcaaaagcc atatagtgat     1740

gagaaattta agctcaattt tgagaactcg actttggcta atggttggga taaaaataaa     1800

gagcctgaca atacggcaat tttatttatc aaagatgata aatattatct gggtgtgatg     1860

aataagaaaa ataacaaaat atttgatgat aaagctatca aagaaaataa aggcgagggt     1920

tataaaaaaa ttgtttataa acttttacct ggcgcaaata aaatgttacc taaggttttc     1980

ttttctgcta aatctataaa attttataat cctagtgaag atatacttag aataagaaat     2040

cattccacac atacaaaaaa tggtagtcct caaaaaggat atgaaaaatt tgagtttaat     2100

attgaagatt gccgaaaatt tatagatttt tataaacagt ctataagtaa gcatccggag     2160

tggaaagatt ttggatttag attttctgat actcaaagat ataattctat agatgaattt     2220

tatagagaag ttgaaaatca aggctacaaa ctaacttttg aaaatatatc agagagctat     2280

attgatagcg tagttaatca gggtaaattg tacctattcc aaatctataa taaagatttt     2340

tcagcttata gcaaagggcg accaaatcta catactttat attggaaagc gctgtttgat     2400

gagagaaatc ttcaagatgt ggtttataag ctaaatggtg aggcagagct tttttatcgt     2460

aaacaatcaa tacctaaaaa aatcactcac ccagctaaag aggcaatagc taataaaaac     2520

aaagataatc ctaaaaaaga gagtgttttt gaatatgatt taatcaaaga taaacgcttt     2580

actgaagata agtttttctt tcactgtcct attacaatca attttaaatc tagtggagct     2640

aataagttta atgatgaaat caatttattg ctaaaagaaa aagcaaatga tgttcatata     2700

ttaagtatag atagaggtga aagacattta gcttactata ctttggtaga tggtaaaggc     2760

aatatcatca aacaagatac tttcaacatc attggtaatg atagaatgaa aacaaactac     2820

catgataagc ttgctgcaat agagaaagat agggattcag ctaggaaaga ctggaaaaag     2880

ataaataaca tcaaagagat gaaagagggc tatctatctc aggtagttca tgaaatagct     2940

aagctagtta tagagtataa tgctattgtg gtttttgagg atttaaattt tggatttaaa     3000

agagggcgtt tcaaggtaga gaagcaggtc tatggaaagt taggaaaaat gctaattgag     3060

aaactaaact atctagtttt caaagataat gagtttgata aaactggggg agtgcttaga     3120

gcttatcagc taacagcacc ttttgagact tttaaaaaga tgggtaaaca aacaggtatt     3180

atctactatg taccagctgg ttttacttca aaaatttgtc ctgtaactgg ttttgtaaat     3240

cagttatatc ctaagtatga aagtgtcagc aaatctcaag agttctttag taagtttgac     3300

aagatttgtt ataaccttga taagggctat tttgagttta gttttgatta taaaaacttt     3360

ggtgacaagg ctgccaaagg caagtggact atagctagct ttgggagtag attgattaac     3420

tttagaaatt cagataaaaa tcataattgg gatactcgag aagtttatcc aactaaagag     3480

ttggagaaat tgctaaaaga ttattctatc gaatatgggc atggcgaatg tatcaaagca     3540

gctatttgcg gtgagagcga caaaaagttt tttgctaagc taactagtgt cctaaatact     3600

atcttacaaa tgcgtaactc aaaaacaggt actgagttag attatctaat ttcaccagta     3660

gcagatgtaa atggcaattt ctttgattcg cgacaggcgc caaaaaatat gcctcaagat     3720

gctgatgcca atggtgctta tcatattggg ctaaaaggtc tgatgctact aggtaggatc     3780

aaaaataatc aagagggcaa aaaactcaat ttggttatca aaaatgaaga gtattttgag     3840

ttcgtgcaga ataggaataa c                                               3861


<210>  34
<211>  1287
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1287)
<223>  Rec-deletion Cpf1 mutant

<400>  34

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val 
                325                 330                 335     


Thr Thr Met Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val 
            340                 345                 350         


Glu Glu Lys Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu 
        355                 360                 365             


Lys Ala Gln Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys 
    370                 375                 380                 


Ser Leu Thr Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile 
385                 390                 395                 400 


Gly Thr Ala Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn 
                405                 410                 415     


Leu Asp Asn Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr 
            420                 425                 430         


Glu Lys Ala Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu 
        435                 440                 445             


Glu Phe Asn Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu 
    450                 455                 460                 


Ile Leu Ala Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala 
465                 470                 475                 480 


Gln Asn Lys Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln 
                485                 490                 495     


Gly Lys Lys Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala 
            500                 505                 510         


Ile Lys Asp Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys 
        515                 520                 525             


Ile Phe His Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys 
    530                 535                 540                 


Asp Glu His Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala 
545                 550                 555                 560 


Asn Ile Val Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys 
                565                 570                 575     


Pro Tyr Ser Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu 
            580                 585                 590         


Ala Asn Gly Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu 
        595                 600                 605             


Phe Ile Lys Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn 
    610                 615                 620                 


Asn Lys Ile Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly 
625                 630                 635                 640 


Tyr Lys Lys Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu 
                645                 650                 655     


Pro Lys Val Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser 
            660                 665                 670         


Glu Asp Ile Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly 
        675                 680                 685             


Ser Pro Gln Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys 
    690                 695                 700                 


Arg Lys Phe Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu 
705                 710                 715                 720 


Trp Lys Asp Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser 
                725                 730                 735     


Ile Asp Glu Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr 
            740                 745                 750         


Phe Glu Asn Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly 
        755                 760                 765             


Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser 
    770                 775                 780                 


Lys Gly Arg Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp 
785                 790                 795                 800 


Glu Arg Asn Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu 
                805                 810                 815     


Leu Phe Tyr Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala 
            820                 825                 830         


Lys Glu Ala Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser 
        835                 840                 845             


Val Phe Glu Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys 
    850                 855                 860                 


Phe Phe Phe His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala 
865                 870                 875                 880 


Asn Lys Phe Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn 
                885                 890                 895     


Asp Val His Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr 
            900                 905                 910         


Tyr Thr Leu Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe 
        915                 920                 925             


Asn Ile Ile Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu 
    930                 935                 940                 


Ala Ala Ile Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys 
945                 950                 955                 960 


Ile Asn Asn Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val 
                965                 970                 975     


His Glu Ile Ala Lys Leu Val Ile Glu Tyr Asn Ala Ile Val Val Phe 
            980                 985                 990         


Glu Asp Leu Asn Phe Gly Phe Lys  Arg Gly Arg Phe Lys  Val Glu Lys 
        995                 1000                 1005             


Gln Val  Tyr Gly Lys Leu Gly  Lys Met Leu Ile Glu  Lys Leu Asn 
    1010                 1015                 1020             


Tyr Leu  Val Phe Lys Asp Asn  Glu Phe Asp Lys Thr  Gly Gly Val 
    1025                 1030                 1035             


Leu Arg  Ala Tyr Gln Leu Thr  Ala Pro Phe Glu Thr  Phe Lys Lys 
    1040                 1045                 1050             


Met Gly  Lys Gln Thr Gly Ile  Ile Tyr Tyr Val Pro  Ala Gly Phe 
    1055                 1060                 1065             


Thr Ser  Lys Ile Cys Pro Val  Thr Gly Phe Val Asn  Gln Leu Tyr 
    1070                 1075                 1080             


Pro Lys  Tyr Glu Ser Val Ser  Lys Ser Gln Glu Phe  Phe Ser Lys 
    1085                 1090                 1095             


Phe Asp  Lys Ile Cys Tyr Asn  Leu Asp Lys Gly Tyr  Phe Glu Phe 
    1100                 1105                 1110             


Ser Phe  Asp Tyr Lys Asn Phe  Gly Asp Lys Ala Ala  Lys Gly Lys 
    1115                 1120                 1125             


Trp Thr  Ile Ala Ser Phe Gly  Ser Arg Leu Ile Asn  Phe Arg Asn 
    1130                 1135                 1140             


Ser Asp  Lys Asn His Asn Trp  Asp Thr Arg Glu Val  Tyr Pro Thr 
    1145                 1150                 1155             


Lys Glu  Leu Glu Lys Leu Leu  Lys Asp Tyr Ser Ile  Glu Tyr Gly 
    1160                 1165                 1170             


His Gly  Glu Cys Ile Lys Ala  Ala Ile Cys Gly Glu  Ser Asp Lys 
    1175                 1180                 1185             


Lys Phe  Phe Ala Lys Leu Thr  Ser Val Leu Asn Thr  Ile Leu Gln 
    1190                 1195                 1200             


Met Arg  Asn Ser Lys Thr Gly  Thr Glu Leu Asp Tyr  Leu Ile Ser 
    1205                 1210                 1215             


Pro Val  Ala Asp Val Asn Gly  Asn Phe Phe Asp Ser  Arg Gln Ala 
    1220                 1225                 1230             


Pro Lys  Asn Met Pro Gln Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1235                 1240                 1245             


Ile Gly  Leu Lys Gly Leu Met  Leu Leu Gly Arg Ile  Lys Asn Asn 
    1250                 1255                 1260             


Gln Glu  Gly Lys Lys Leu Asn  Leu Val Ile Lys Asn  Glu Glu Tyr 
    1265                 1270                 1275             


Phe Glu  Phe Val Gln Asn Arg  Asn Asn 
    1280                 1285         


<210>  35
<211>  3903
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3903)
<223>  Rec-substitution Cpf1 mutant

<400>  35
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttg gtggtggagc cggtgcaagt gctggaggat ctggatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat     3000

gctattgtgg tttttgagga tttaaatttt ggatttaaaa gagggcgttt caaggtagag     3060

aagcaggtct atcaaaagtt agaaaaaatg ctaattgaga aactaaacta tctagttttc     3120

aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct     3180

tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt     3240

tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa     3300

agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat     3360

aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc     3420

aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat     3480

cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat     3540

tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac     3600

aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca     3660

aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc     3720

tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat     3780

catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa     3840

aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac     3900

taa                                                                   3903


<210>  36
<211>  1300
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  Rec-substitution Cpf1 mutant

<400>  36

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Gly Gly Gly Ala Gly Ala Ser Ala Gly Gly Ser Gly Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Glu Asp Leu 
        995                 1000                 1005             


Asn Phe  Gly Phe Lys Arg Gly  Arg Phe Lys Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  37
<211>  3903
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3903)
<223>  LID-substitution Cpf1 mutant

<400>  37
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat agagtataat     3000

gctattgtgg tttttggtgg tggagccggt ggagcagctg gagggggagc cggtgtagag     3060

aagcaggtct atcaaaagtt agaaaaaatg ctaattgaga aactaaacta tctagttttc     3120

aaagataatg agtttgataa aactggggga gtgcttagag cttatcagct aacagcacct     3180

tttgagactt ttaaaaagat gggtaaacaa acaggtatta tctactatgt accagctggt     3240

tttacttcaa aaatttgtcc tgtaactggt tttgtaaatc agttatatcc taagtatgaa     3300

agtgtcagca aatctcaaga gttctttagt aagtttgaca agatttgtta taaccttgat     3360

aagggctatt ttgagtttag ttttgattat aaaaactttg gtgacaaggc tgccaaaggc     3420

aagtggacta tagctagctt tgggagtaga ttgattaact ttagaaattc agataaaaat     3480

cataattggg atactcgaga agtttatcca actaaagagt tggagaaatt gctaaaagat     3540

tattctatcg aatatgggca tggcgaatgt atcaaagcag ctatttgcgg tgagagcgac     3600

aaaaagtttt ttgctaagct aactagtgtc ctaaatacta tcttacaaat gcgtaactca     3660

aaaacaggta ctgagttaga ttatctaatt tcaccagtag cagatgtaaa tggcaatttc     3720

tttgattcgc gacaggcgcc aaaaaatatg cctcaagatg ctgatgccaa tggtgcttat     3780

catattgggc taaaaggtct gatgctacta ggtaggatca aaaataatca agagggcaaa     3840

aaactcaatt tggttatcaa aaatgaagag tattttgagt tcgtgcagaa taggaataac     3900

taa                                                                   3903


<210>  38
<211>  1300
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1300)
<223>  LID-substitution Cpf1 mutant

<400>  38

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Gly Gly Gly 
        995                 1000                 1005             


Ala Gly  Gly Ala Ala Gly Gly  Gly Ala Gly Val Glu  Lys Gln Val 
    1010                 1015                 1020             


Tyr Gln  Lys Leu Glu Lys Met  Leu Ile Glu Lys Leu  Asn Tyr Leu 
    1025                 1030                 1035             


Val Phe  Lys Asp Asn Glu Phe  Asp Lys Thr Gly Gly  Val Leu Arg 
    1040                 1045                 1050             


Ala Tyr  Gln Leu Thr Ala Pro  Phe Glu Thr Phe Lys  Lys Met Gly 
    1055                 1060                 1065             


Lys Gln  Thr Gly Ile Ile Tyr  Tyr Val Pro Ala Gly  Phe Thr Ser 
    1070                 1075                 1080             


Lys Ile  Cys Pro Val Thr Gly  Phe Val Asn Gln Leu  Tyr Pro Lys 
    1085                 1090                 1095             


Tyr Glu  Ser Val Ser Lys Ser  Gln Glu Phe Phe Ser  Lys Phe Asp 
    1100                 1105                 1110             


Lys Ile  Cys Tyr Asn Leu Asp  Lys Gly Tyr Phe Glu  Phe Ser Phe 
    1115                 1120                 1125             


Asp Tyr  Lys Asn Phe Gly Asp  Lys Ala Ala Lys Gly  Lys Trp Thr 
    1130                 1135                 1140             


Ile Ala  Ser Phe Gly Ser Arg  Leu Ile Asn Phe Arg  Asn Ser Asp 
    1145                 1150                 1155             


Lys Asn  His Asn Trp Asp Thr  Arg Glu Val Tyr Pro  Thr Lys Glu 
    1160                 1165                 1170             


Leu Glu  Lys Leu Leu Lys Asp  Tyr Ser Ile Glu Tyr  Gly His Gly 
    1175                 1180                 1185             


Glu Cys  Ile Lys Ala Ala Ile  Cys Gly Glu Ser Asp  Lys Lys Phe 
    1190                 1195                 1200             


Phe Ala  Lys Leu Thr Ser Val  Leu Asn Thr Ile Leu  Gln Met Arg 
    1205                 1210                 1215             


Asn Ser  Lys Thr Gly Thr Glu  Leu Asp Tyr Leu Ile  Ser Pro Val 
    1220                 1225                 1230             


Ala Asp  Val Asn Gly Asn Phe  Phe Asp Ser Arg Gln  Ala Pro Lys 
    1235                 1240                 1245             


Asn Met  Pro Gln Asp Ala Asp  Ala Asn Gly Ala Tyr  His Ile Gly 
    1250                 1255                 1260             


Leu Lys  Gly Leu Met Leu Leu  Gly Arg Ile Lys Asn  Asn Gln Glu 
    1265                 1270                 1275             


Gly Lys  Lys Leu Asn Leu Val  Ile Lys Asn Glu Glu  Tyr Phe Glu 
    1280                 1285                 1290             


Phe Val  Gln Asn Arg Asn Asn  
    1295                 1300 


<210>  39
<211>  3814
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence

<400>  39
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag aaaaggtata      900

aatgaatata taaatctata ctcacagcaa ataaatgata aaacactcaa aaaatataaa      960

atgagtgttt tatttaagca aattttaagt gatacagaat ctaaatcttt tgtaattgat     1020

aagttagaag atgatagtga tgtagttaca acgatgcaaa gtttttatga gcaaatagca     1080

gcttttaaaa cagtagaaga aaaatctatt aaagaaacac tatctttatt atttgatgat     1140

ttaaaagctc aaaaacttga tttgagtaaa atttatttta aaaatgataa atctcttact     1200

gatctatcac aacaagtttt tgatgattat agtgttattg gtacagcggt actagaatat     1260

ataactcaac aaatagcacc taaaaatctt gataacccta gtaagaaaga gcaagaatta     1320

atagccaaaa aaactgaaaa agcaaaatac ttatctctag aaactataaa gcttgcctta     1380

gaagaattta ataagcatag agatatagat aaacagtgta ggtttgaaga aatacttgca     1440

aactttgcgg ctattccgat gatatttgat gaaatagctc aaaacaaaga caatttggca     1500

cagatatcta tcaaatatca aaatcaaggt aaaaaagacc tacttcaagc tagtgcggaa     1560

gatgatgtta aagctatcaa ggatctttta gatcaaacta ataatctctt acataaacta     1620

aaaatatttc atattagtca gtcagaagat aaggcaaata ttttagacaa ggatgagcat     1680

ttttatctag tatttgagga gtgctacttt gagctagcga atatagtgcc tctttataac     1740

aaaattagaa actatataac tcaaaagcca tatagtgatg agaaatttaa gctcaatttt     1800

gagaactcga ctttggctaa tggttgggat aaaaataaag agcctgacaa tacggcaatt     1860

ttatttatca aagatgataa atattatctg ggtgtgatga ataagaaaaa taacaaaata     1920

tttgatgata aagctatcaa agaaaataaa ggcgagggtt ataaaaaaat tgtttataaa     1980

cttttacctg gcgcaaataa aatgttacct aaggttttct tttctgctaa atctataaaa     2040

ttttataatc ctagtgaaga tatacttaga ataagaaatc attccacaca tacaaaaaat     2100

ggtagtcctc aaaaaggata tgaaaaattt gagtttaata ttgaagattg ccgaaaattt     2160

atagattttt ataaacagtc tataagtaag catccggagt ggaaagattt tggatttaga     2220

ttttctgata ctcaaagata taattctata gatgaatttt atagagaagt tgaaaatcaa     2280

ggctacaaac taacttttga aaatatatca gagagctata ttgatagcgt agttaatcag     2340

ggtaaattgt acctattcca aatctataat aaagattttt cagcttatag caaagggcga     2400

ccaaatctac atactttata ttggaaagcg ctgtttgatg agagaaatct tcaagatgtg     2460

gtttataagc taaatggtga ggcagagctt ttttatcgta aacaatcaat acctaaaaaa     2520

atcactcacc cagctaaaga ggcaatagct aataaaaaca aagataatcc taaaaaagag     2580

agtgtttttg aatatgattt aatcaaagat aaacgcttta ctgaagataa gtttttcttt     2640

cactgtccta ttacaatcaa ttttaaatct agtggagcta ataagtttaa tgatgaaatc     2700

aatttattgc taaaagaaaa agcaaatgat gttcatatat taagtataga tagaggtgaa     2760

agacatttag cttactatac tttggtagat ggtaaaggca atatcatcaa acaagatact     2820

ttcaacatca ttggtaatga tagaatgaaa acaaactacc atgataagct tgctgcaata     2880

gagaaagata gggattcagc taggaaagac tggaaaaaga taaataacat caaagagatg     2940

aaagagggct atctatctca ggtagttcat gaaatagcta agctagttat tagaaaaaat     3000

gctaattgag aaactaaact atctagtttt caaagataat gagtttgata aaactggggg     3060

agtgcttaga gcttatcagc taacagcacc ttttgagact tttaaaaaga tgggtaaaca     3120

aacaggtatt atctactatg taccagctgg ttttacttca aaaatttgtc ctgtaactgg     3180

ttttgtaaat cagttatatc ctaagtatga aagtgtcagc aaatctcaag agttctttag     3240

taagtttgac aagatttgtt ataaccttga taagggctat tttgagttta gttttgatta     3300

taaaaacttt ggtgacaagg ctgccaaagg caagtggact atagctagct ttgggagtag     3360

attgattaac tttagaaatt cagataaaaa tcataattgg gatactcgag aagtttatcc     3420

aactaaagag ttggagaaat tgctaaaaga ttattctatc gaatatgggc atggcgaatg     3480

tatcaaagca gctatttgcg gtgagagcga caaaaagttt tttgctaagc taactagtgt     3540

cctaaatact atcttacaaa tgcgtaactc aaaaacaggt actgagttag attatctaat     3600

ttcaccagta gcagatgtaa atggcaattt ctttgattcg cgacaggcgc caaaaaatat     3660

gcctcaagat gctgatgcca atggtgctta tcatattggg ctaaaaggtc tgatgctact     3720

aggtaggatc aaaaataatc aagagggcaa aaaactcaat ttggttatca aaaatgaaga     3780

gtattttgag ttcgtgcaga ataggaataa ctaa                                 3814


<210>  40
<211>  1287
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1287)
<223>  LID-deletion Cpf1 mutant

<400>  40

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Lys Gly Ile Asn Glu Tyr Ile 
    290                 295                 300                 


Asn Leu Tyr Ser Gln Gln Ile Asn Asp Lys Thr Leu Lys Lys Tyr Lys 
305                 310                 315                 320 


Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr Glu Ser Lys Ser 
                325                 330                 335     


Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val Val Thr Thr Met 
            340                 345                 350         


Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr Val Glu Glu Lys 
        355                 360                 365             


Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp Leu Lys Ala Gln 
    370                 375                 380                 


Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp Lys Ser Leu Thr 
385                 390                 395                 400 


Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val Ile Gly Thr Ala 
                405                 410                 415     


Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys Asn Leu Asp Asn 
            420                 425                 430         


Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys Thr Glu Lys Ala 
        435                 440                 445             


Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu Glu Glu Phe Asn 
    450                 455                 460                 


Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu Glu Ile Leu Ala 
465                 470                 475                 480 


Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile Ala Gln Asn Lys 
                485                 490                 495     


Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn Gln Gly Lys Lys 
            500                 505                 510         


Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys Ala Ile Lys Asp 
        515                 520                 525             


Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu Lys Ile Phe His 
    530                 535                 540                 


Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp Lys Asp Glu His 
545                 550                 555                 560 


Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu Ala Asn Ile Val 
                565                 570                 575     


Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln Lys Pro Tyr Ser 
            580                 585                 590         


Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr Leu Ala Asn Gly 
        595                 600                 605             


Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile Leu Phe Ile Lys 
    610                 615                 620                 


Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys Asn Asn Lys Ile 
625                 630                 635                 640 


Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu Gly Tyr Lys Lys 
                645                 650                 655     


Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met Leu Pro Lys Val 
            660                 665                 670         


Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro Ser Glu Asp Ile 
        675                 680                 685             


Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn Gly Ser Pro Gln 
    690                 695                 700                 


Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp Cys Arg Lys Phe 
705                 710                 715                 720 


Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro Glu Trp Lys Asp 
                725                 730                 735     


Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn Ser Ile Asp Glu 
            740                 745                 750         


Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu Thr Phe Glu Asn 
        755                 760                 765             


Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln Gly Lys Leu Tyr 
    770                 775                 780                 


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr Ser Lys Gly Arg 
785                 790                 795                 800 


Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe Asp Glu Arg Asn 
                805                 810                 815     


Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala Glu Leu Phe Tyr 
            820                 825                 830         


Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro Ala Lys Glu Ala 
        835                 840                 845             


Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu Ser Val Phe Glu 
    850                 855                 860                 


Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly Ala Asn Lys Phe 
                885                 890                 895     


Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala Asn Asp Val His 
            900                 905                 910         


Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala Tyr Tyr Thr Leu 
        915                 920                 925             


Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr Phe Asn Ile Ile 
    930                 935                 940                 


Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys Leu Ala Ala Ile 
945                 950                 955                 960 


Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys Lys Ile Asn Asn 
                965                 970                 975     


Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val Val His Glu Ile 
            980                 985                 990         


Ala Lys Leu Val Ile Glu Tyr Asn  Ala Ile Val Val Phe  Val Glu Lys 
        995                 1000                 1005             


Gln Val  Tyr Gln Lys Leu Glu  Lys Met Leu Ile Glu  Lys Leu Asn 
    1010                 1015                 1020             


Tyr Leu  Val Phe Lys Asp Asn  Glu Phe Asp Lys Thr  Gly Gly Val 
    1025                 1030                 1035             


Leu Arg  Ala Tyr Gln Leu Thr  Ala Pro Phe Glu Thr  Phe Lys Lys 
    1040                 1045                 1050             


Met Gly  Lys Gln Thr Gly Ile  Ile Tyr Tyr Val Pro  Ala Gly Phe 
    1055                 1060                 1065             


Thr Ser  Lys Ile Cys Pro Val  Thr Gly Phe Val Asn  Gln Leu Tyr 
    1070                 1075                 1080             


Pro Lys  Tyr Glu Ser Val Ser  Lys Ser Gln Glu Phe  Phe Ser Lys 
    1085                 1090                 1095             


Phe Asp  Lys Ile Cys Tyr Asn  Leu Asp Lys Gly Tyr  Phe Glu Phe 
    1100                 1105                 1110             


Ser Phe  Asp Tyr Lys Asn Phe  Gly Asp Lys Ala Ala  Lys Gly Lys 
    1115                 1120                 1125             


Trp Thr  Ile Ala Ser Phe Gly  Ser Arg Leu Ile Asn  Phe Arg Asn 
    1130                 1135                 1140             


Ser Asp  Lys Asn His Asn Trp  Asp Thr Arg Glu Val  Tyr Pro Thr 
    1145                 1150                 1155             


Lys Glu  Leu Glu Lys Leu Leu  Lys Asp Tyr Ser Ile  Glu Tyr Gly 
    1160                 1165                 1170             


His Gly  Glu Cys Ile Lys Ala  Ala Ile Cys Gly Glu  Ser Asp Lys 
    1175                 1180                 1185             


Lys Phe  Phe Ala Lys Leu Thr  Ser Val Leu Asn Thr  Ile Leu Gln 
    1190                 1195                 1200             


Met Arg  Asn Ser Lys Thr Gly  Thr Glu Leu Asp Tyr  Leu Ile Ser 
    1205                 1210                 1215             


Pro Val  Ala Asp Val Asn Gly  Asn Phe Phe Asp Ser  Arg Gln Ala 
    1220                 1225                 1230             


Pro Lys  Asn Met Pro Gln Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1235                 1240                 1245             


Ile Gly  Leu Lys Gly Leu Met  Leu Leu Gly Arg Ile  Lys Asn Asn 
    1250                 1255                 1260             


Gln Glu  Gly Lys Lys Leu Asn  Leu Val Ile Lys Asn  Glu Glu Tyr 
    1265                 1270                 1275             


Phe Glu  Phe Val Gln Asn Arg  Asn Asn 
    1280                 1285         


<210>  41
<211>  3867
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(3867)
<223>  Finger-deletion Cpf1 mutant

<400>  41
atgtcaattt atcaagaatt tgttaataaa tatagtttaa gtaaaactct aagatttgag       60

ttaatcccac agggtaaaac acttgaaaac ataaaagcaa gaggtttgat tttagatgat      120

gagaaaagag ctaaagacta caaaaaggct aaacaaataa ttgataaata tcatcagttt      180

tttatagagg agatattaag ttcggtttgt attagcgaag atttattaca aaactattct      240

gatgtttatt ttaaacttaa aaagagtgat gatgataatc tacaaaaaga ttttaaaagt      300

gcaaaagata cgataaagaa acaaatatct gaatatataa aggactcaga gaaatttaag      360

aatttgttta atcaaaacct tatcgatgct aaaaaagggc aagagtcaga tttaattcta      420

tggctaaagc aatctaagga taatggtata gaactattta aagccaatag tgatatcaca      480

gatatagatg aggcgttaga aataatcaaa tcttttaaag gttggacaac ttattttaag      540

ggttttcatg aaaatagaaa aaatgtttat agtagcaatg atattcctac atctattatt      600

tataggatag tagatgataa tttgcctaaa tttctagaaa ataaagctaa gtatgagagt      660

ttaaaagaca aagctccaga agctataaac tatgaacaaa ttaaaaaaga tttggcagaa      720

gagctaacct ttgatattga ctacaaaaca tctgaagtta atcaaagagt tttttcactt      780

gatgaagttt ttgagatagc aaactttaat aattatctaa atcaaagtgg tattactaaa      840

tttaatacta ttattggtgg taaatttgta aatggtgaaa atacaaagag acaaataaat      900

gataaaacac tcaaaaaata taaaatgagt gttttattta agcaaatttt aagtgataca      960

gaatctaaat cttttgtaat tgataagtta gaagatgata gtgatgtagt tacaacgatg     1020

caaagttttt atgagcaaat agcagctttt aaaacagtag aagaaaaatc tattaaagaa     1080

acactatctt tattatttga tgatttaaaa gctcaaaaac ttgatttgag taaaatttat     1140

tttaaaaatg ataaatctct tactgatcta tcacaacaag tttttgatga ttatagtgtt     1200

attggtacag cggtactaga atatataact caacaaatag cacctaaaaa tcttgataac     1260

cctagtaaga aagagcaaga attaatagcc aaaaaaactg aaaaagcaaa atacttatct     1320

ctagaaacta taaagcttgc cttagaagaa tttaataagc atagagatat agataaacag     1380

tgtaggtttg aagaaatact tgcaaacttt gcggctattc cgatgatatt tgatgaaata     1440

gctcaaaaca aagacaattt ggcacagata tctatcaaat atcaaaatca aggtaaaaaa     1500

gacctacttc aagctagtgc ggaagatgat gttaaagcta tcaaggatct tttagatcaa     1560

actaataatc tcttacataa actaaaaata tttcatatta gtcagtcaga agataaggca     1620

aatattttag acaaggatga gcatttttat ctagtatttg aggagtgcta ctttgagcta     1680

gcgaatatag tgcctcttta taacaaaatt agaaactata taactcaaaa gccatatagt     1740

gatgagaaat ttaagctcaa ttttgagaac tcgactttgg ctaatggttg ggataaaaat     1800

aaagagcctg acaatacggc aattttattt atcaaagatg ataaatatta tctgggtgtg     1860

atgaataaga aaaataacaa aatatttgat gataaagcta tcaaagaaaa taaaggcgag     1920

ggttataaaa aaattgttta taaactttta cctggcgcaa ataaaatgtt acctaaggtt     1980

ttcttttctg ctaaatctat aaaattttat aatcctagtg aagatatact tagaataaga     2040

aatcattcca cacatacaaa aaatggtagt cctcaaaaag gatatgaaaa atttgagttt     2100

aatattgaag attgccgaaa atttatagat ttttataaac agtctataag taagcatccg     2160

gagtggaaag attttggatt tagattttct gatactcaaa gatataattc tatagatgaa     2220

ttttatagag aagttgaaaa tcaaggctac aaactaactt ttgaaaatat atcagagagc     2280

tatattgata gcgtagttaa tcagggtaaa ttgtacctat tccaaatcta taataaagat     2340

ttttcagctt atagcaaagg gcgaccaaat ctacatactt tatattggaa agcgctgttt     2400

gatgagagaa atcttcaaga tgtggtttat aagctaaatg gtgaggcaga gcttttttat     2460

cgtaaacaat caatacctaa aaaaatcact cacccagcta aagaggcaat agctaataaa     2520

aacaaagata atcctaaaaa agagagtgtt tttgaatatg atttaatcaa agataaacgc     2580

tttactgaag ataagttttt ctttcactgt cctattacaa tcaattttaa atctagtgga     2640

gctaataagt ttaatgatga aatcaattta ttgctaaaag aaaaagcaaa tgatgttcat     2700

atattaagta tagatagagg tgaaagacat ttagcttact atactttggt agatggtaaa     2760

ggcaatatca tcaaacaaga tactttcaac atcattggta atgatagaat gaaaacaaac     2820

taccatgata agcttgctgc aatagagaaa gatagggatt cagctaggaa agactggaaa     2880

aagataaata acatcaaaga gatgaaagag ggctatctat ctcaggtagt tcatgaaata     2940

gctaagctag ttatagagta taatgctatt gtggtttttg aggatttaaa ttttggattt     3000

aaaagagggc gtttcaaggt agagaagcag gtctatcaaa agttagaaaa aatgctaatt     3060

gagaaactaa actatctagt tttcaaagat aatgagtttg ataaaactgg gggagtgctt     3120

agagcttatc agctaacagc accttttgag acttttaaaa agatgggtaa acaaacaggt     3180

attatctact atgtaccagc tggttttact tcaaaaattt gtcctgtaac tggttttgta     3240

aatcagttat atcctaagta tgaaagtgtc agcaaatctc aagagttctt tagtaagttt     3300

gacaagattt gttataacct tgataagggc tattttgagt ttagttttga ttataaaaac     3360

tttggtgaca aggctgccaa aggcaagtgg actatagcta gctttgggag tagattgatt     3420

aactttagaa attcagataa aaatcataat tgggatactc gagaagttta tccaactaaa     3480

gagttggaga aattgctaaa agattattct atcgaatatg ggcatggcga atgtatcaaa     3540

gcagctattt gcggtgagag cgacaaaaag ttttttgcta agctaactag tgtcctaaat     3600

actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt tagattatct aatttcacca     3660

gtagcagatg taaatggcaa tttctttgat tcgcgacagg cgccaaaaaa tatgcctcaa     3720

gatgctgatg ccaatggtgc ttatcatatt gggctaaaag gtctgatgct actaggtagg     3780

atcaaaaata atcaagaggg caaaaaactc aatttggtta tcaaaaatga agagtatttt     3840

gagttcgtgc agaataggaa taactaa                                         3867


<210>  42
<211>  1288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1288)
<223>  Finger-deletion Cpf1 mutant

<400>  42

Met Ser Ile Tyr Gln Glu Phe Val Asn Lys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Glu Asn Ile Lys 
            20                  25                  30          


Ala Arg Gly Leu Ile Leu Asp Asp Glu Lys Arg Ala Lys Asp Tyr Lys 
        35                  40                  45              


Lys Ala Lys Gln Ile Ile Asp Lys Tyr His Gln Phe Phe Ile Glu Glu 
    50                  55                  60                  


Ile Leu Ser Ser Val Cys Ile Ser Glu Asp Leu Leu Gln Asn Tyr Ser 
65                  70                  75                  80  


Asp Val Tyr Phe Lys Leu Lys Lys Ser Asp Asp Asp Asn Leu Gln Lys 
                85                  90                  95      


Asp Phe Lys Ser Ala Lys Asp Thr Ile Lys Lys Gln Ile Ser Glu Tyr 
            100                 105                 110         


Ile Lys Asp Ser Glu Lys Phe Lys Asn Leu Phe Asn Gln Asn Leu Ile 
        115                 120                 125             


Asp Ala Lys Lys Gly Gln Glu Ser Asp Leu Ile Leu Trp Leu Lys Gln 
    130                 135                 140                 


Ser Lys Asp Asn Gly Ile Glu Leu Phe Lys Ala Asn Ser Asp Ile Thr 
145                 150                 155                 160 


Asp Ile Asp Glu Ala Leu Glu Ile Ile Lys Ser Phe Lys Gly Trp Thr 
                165                 170                 175     


Thr Tyr Phe Lys Gly Phe His Glu Asn Arg Lys Asn Val Tyr Ser Ser 
            180                 185                 190         


Asn Asp Ile Pro Thr Ser Ile Ile Tyr Arg Ile Val Asp Asp Asn Leu 
        195                 200                 205             


Pro Lys Phe Leu Glu Asn Lys Ala Lys Tyr Glu Ser Leu Lys Asp Lys 
    210                 215                 220                 


Ala Pro Glu Ala Ile Asn Tyr Glu Gln Ile Lys Lys Asp Leu Ala Glu 
225                 230                 235                 240 


Glu Leu Thr Phe Asp Ile Asp Tyr Lys Thr Ser Glu Val Asn Gln Arg 
                245                 250                 255     


Val Phe Ser Leu Asp Glu Val Phe Glu Ile Ala Asn Phe Asn Asn Tyr 
            260                 265                 270         


Leu Asn Gln Ser Gly Ile Thr Lys Phe Asn Thr Ile Ile Gly Gly Lys 
        275                 280                 285             


Phe Val Asn Gly Glu Asn Thr Lys Arg Gln Ile Asn Asp Lys Thr Leu 
    290                 295                 300                 


Lys Lys Tyr Lys Met Ser Val Leu Phe Lys Gln Ile Leu Ser Asp Thr 
305                 310                 315                 320 


Glu Ser Lys Ser Phe Val Ile Asp Lys Leu Glu Asp Asp Ser Asp Val 
                325                 330                 335     


Val Thr Thr Met Gln Ser Phe Tyr Glu Gln Ile Ala Ala Phe Lys Thr 
            340                 345                 350         


Val Glu Glu Lys Ser Ile Lys Glu Thr Leu Ser Leu Leu Phe Asp Asp 
        355                 360                 365             


Leu Lys Ala Gln Lys Leu Asp Leu Ser Lys Ile Tyr Phe Lys Asn Asp 
    370                 375                 380                 


Lys Ser Leu Thr Asp Leu Ser Gln Gln Val Phe Asp Asp Tyr Ser Val 
385                 390                 395                 400 


Ile Gly Thr Ala Val Leu Glu Tyr Ile Thr Gln Gln Ile Ala Pro Lys 
                405                 410                 415     


Asn Leu Asp Asn Pro Ser Lys Lys Glu Gln Glu Leu Ile Ala Lys Lys 
            420                 425                 430         


Thr Glu Lys Ala Lys Tyr Leu Ser Leu Glu Thr Ile Lys Leu Ala Leu 
        435                 440                 445             


Glu Glu Phe Asn Lys His Arg Asp Ile Asp Lys Gln Cys Arg Phe Glu 
    450                 455                 460                 


Glu Ile Leu Ala Asn Phe Ala Ala Ile Pro Met Ile Phe Asp Glu Ile 
465                 470                 475                 480 


Ala Gln Asn Lys Asp Asn Leu Ala Gln Ile Ser Ile Lys Tyr Gln Asn 
                485                 490                 495     


Gln Gly Lys Lys Asp Leu Leu Gln Ala Ser Ala Glu Asp Asp Val Lys 
            500                 505                 510         


Ala Ile Lys Asp Leu Leu Asp Gln Thr Asn Asn Leu Leu His Lys Leu 
        515                 520                 525             


Lys Ile Phe His Ile Ser Gln Ser Glu Asp Lys Ala Asn Ile Leu Asp 
    530                 535                 540                 


Lys Asp Glu His Phe Tyr Leu Val Phe Glu Glu Cys Tyr Phe Glu Leu 
545                 550                 555                 560 


Ala Asn Ile Val Pro Leu Tyr Asn Lys Ile Arg Asn Tyr Ile Thr Gln 
                565                 570                 575     


Lys Pro Tyr Ser Asp Glu Lys Phe Lys Leu Asn Phe Glu Asn Ser Thr 
            580                 585                 590         


Leu Ala Asn Gly Trp Asp Lys Asn Lys Glu Pro Asp Asn Thr Ala Ile 
        595                 600                 605             


Leu Phe Ile Lys Asp Asp Lys Tyr Tyr Leu Gly Val Met Asn Lys Lys 
    610                 615                 620                 


Asn Asn Lys Ile Phe Asp Asp Lys Ala Ile Lys Glu Asn Lys Gly Glu 
625                 630                 635                 640 


Gly Tyr Lys Lys Ile Val Tyr Lys Leu Leu Pro Gly Ala Asn Lys Met 
                645                 650                 655     


Leu Pro Lys Val Phe Phe Ser Ala Lys Ser Ile Lys Phe Tyr Asn Pro 
            660                 665                 670         


Ser Glu Asp Ile Leu Arg Ile Arg Asn His Ser Thr His Thr Lys Asn 
        675                 680                 685             


Gly Ser Pro Gln Lys Gly Tyr Glu Lys Phe Glu Phe Asn Ile Glu Asp 
    690                 695                 700                 


Cys Arg Lys Phe Ile Asp Phe Tyr Lys Gln Ser Ile Ser Lys His Pro 
705                 710                 715                 720 


Glu Trp Lys Asp Phe Gly Phe Arg Phe Ser Asp Thr Gln Arg Tyr Asn 
                725                 730                 735     


Ser Ile Asp Glu Phe Tyr Arg Glu Val Glu Asn Gln Gly Tyr Lys Leu 
            740                 745                 750         


Thr Phe Glu Asn Ile Ser Glu Ser Tyr Ile Asp Ser Val Val Asn Gln 
        755                 760                 765             


Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ser Ala Tyr 
    770                 775                 780                 


Ser Lys Gly Arg Pro Asn Leu His Thr Leu Tyr Trp Lys Ala Leu Phe 
785                 790                 795                 800 


Asp Glu Arg Asn Leu Gln Asp Val Val Tyr Lys Leu Asn Gly Glu Ala 
                805                 810                 815     


Glu Leu Phe Tyr Arg Lys Gln Ser Ile Pro Lys Lys Ile Thr His Pro 
            820                 825                 830         


Ala Lys Glu Ala Ile Ala Asn Lys Asn Lys Asp Asn Pro Lys Lys Glu 
        835                 840                 845             


Ser Val Phe Glu Tyr Asp Leu Ile Lys Asp Lys Arg Phe Thr Glu Asp 
    850                 855                 860                 


Lys Phe Phe Phe His Cys Pro Ile Thr Ile Asn Phe Lys Ser Ser Gly 
865                 870                 875                 880 


Ala Asn Lys Phe Asn Asp Glu Ile Asn Leu Leu Leu Lys Glu Lys Ala 
                885                 890                 895     


Asn Asp Val His Ile Leu Ser Ile Asp Arg Gly Glu Arg His Leu Ala 
            900                 905                 910         


Tyr Tyr Thr Leu Val Asp Gly Lys Gly Asn Ile Ile Lys Gln Asp Thr 
        915                 920                 925             


Phe Asn Ile Ile Gly Asn Asp Arg Met Lys Thr Asn Tyr His Asp Lys 
    930                 935                 940                 


Leu Ala Ala Ile Glu Lys Asp Arg Asp Ser Ala Arg Lys Asp Trp Lys 
945                 950                 955                 960 


Lys Ile Asn Asn Ile Lys Glu Met Lys Glu Gly Tyr Leu Ser Gln Val 
                965                 970                 975     


Val His Glu Ile Ala Lys Leu Val Ile Glu Tyr Asn Ala Ile Val Val 
            980                 985                 990         


Phe Glu Asp Leu Asn Phe Gly Phe  Lys Arg Gly Arg Phe  Lys Val Glu 
        995                 1000                 1005             


Lys Gln  Val Tyr Gln Lys Leu  Glu Lys Met Leu Ile  Glu Lys Leu 
    1010                 1015                 1020             


Asn Tyr  Leu Val Phe Lys Asp  Asn Glu Phe Asp Lys  Thr Gly Gly 
    1025                 1030                 1035             


Val Leu  Arg Ala Tyr Gln Leu  Thr Ala Pro Phe Glu  Thr Phe Lys 
    1040                 1045                 1050             


Lys Met  Gly Lys Gln Thr Gly  Ile Ile Tyr Tyr Val  Pro Ala Gly 
    1055                 1060                 1065             


Phe Thr  Ser Lys Ile Cys Pro  Val Thr Gly Phe Val  Asn Gln Leu 
    1070                 1075                 1080             


Tyr Pro  Lys Tyr Glu Ser Val  Ser Lys Ser Gln Glu  Phe Phe Ser 
    1085                 1090                 1095             


Lys Phe  Asp Lys Ile Cys Tyr  Asn Leu Asp Lys Gly  Tyr Phe Glu 
    1100                 1105                 1110             


Phe Ser  Phe Asp Tyr Lys Asn  Phe Gly Asp Lys Ala  Ala Lys Gly 
    1115                 1120                 1125             


Lys Trp  Thr Ile Ala Ser Phe  Gly Ser Arg Leu Ile  Asn Phe Arg 
    1130                 1135                 1140             


Asn Ser  Asp Lys Asn His Asn  Trp Asp Thr Arg Glu  Val Tyr Pro 
    1145                 1150                 1155             


Thr Lys  Glu Leu Glu Lys Leu  Leu Lys Asp Tyr Ser  Ile Glu Tyr 
    1160                 1165                 1170             


Gly His  Gly Glu Cys Ile Lys  Ala Ala Ile Cys Gly  Glu Ser Asp 
    1175                 1180                 1185             


Lys Lys  Phe Phe Ala Lys Leu  Thr Ser Val Leu Asn  Thr Ile Leu 
    1190                 1195                 1200             


Gln Met  Arg Asn Ser Lys Thr  Gly Thr Glu Leu Asp  Tyr Leu Ile 
    1205                 1210                 1215             


Ser Pro  Val Ala Asp Val Asn  Gly Asn Phe Phe Asp  Ser Arg Gln 
    1220                 1225                 1230             


Ala Pro  Lys Asn Met Pro Gln  Asp Ala Asp Ala Asn  Gly Ala Tyr 
    1235                 1240                 1245             


His Ile  Gly Leu Lys Gly Leu  Met Leu Leu Gly Arg  Ile Lys Asn 
    1250                 1255                 1260             


Asn Gln  Glu Gly Lys Lys Leu  Asn Leu Val Ile Lys  Asn Glu Glu 
    1265                 1270                 1275             


Tyr Phe  Glu Phe Val Gln Asn  Arg Asn Asn 
    1280                 1285             


<210>  43
<211>  1238
<212>  PRT
<213>  Candidatus Methanoplasma termitum

<400>  43

Met Asn Asn Tyr Asp Glu Phe Thr Lys Leu Tyr Pro Ile Gln Lys Thr 
1               5                   10                  15      


Ile Arg Phe Glu Leu Lys Pro Gln Gly Arg Thr Met Glu His Leu Glu 
            20                  25                  30          


Thr Phe Asn Phe Phe Glu Glu Asp Arg Asp Arg Ala Glu Lys Tyr Lys 
        35                  40                  45              


Ile Leu Lys Glu Ala Ile Asp Glu Tyr His Lys Lys Phe Ile Asp Glu 
    50                  55                  60                  


His Leu Thr Asn Met Ser Leu Asp Trp Asn Ser Leu Lys Gln Ile Ser 
65                  70                  75                  80  


Glu Lys Tyr Tyr Lys Ser Arg Glu Glu Lys Asp Lys Lys Val Phe Leu 
                85                  90                  95      


Ser Glu Gln Lys Arg Met Arg Gln Glu Ile Val Ser Glu Phe Lys Lys 
            100                 105                 110         


Asp Asp Arg Phe Lys Asp Leu Phe Ser Lys Lys Leu Phe Ser Glu Leu 
        115                 120                 125             


Leu Lys Glu Glu Ile Tyr Lys Lys Gly Asn His Gln Glu Ile Asp Ala 
    130                 135                 140                 


Leu Lys Ser Phe Asp Lys Phe Ser Gly Tyr Phe Ile Gly Leu His Glu 
145                 150                 155                 160 


Asn Arg Lys Asn Met Tyr Ser Asp Gly Asp Glu Ile Thr Ala Ile Ser 
                165                 170                 175     


Asn Arg Ile Val Asn Glu Asn Phe Pro Lys Phe Leu Asp Asn Leu Gln 
            180                 185                 190         


Lys Tyr Gln Glu Ala Arg Lys Lys Tyr Pro Glu Trp Ile Ile Lys Ala 
        195                 200                 205             


Glu Ser Ala Leu Val Ala His Asn Ile Lys Met Asp Glu Val Phe Ser 
    210                 215                 220                 


Leu Glu Tyr Phe Asn Lys Val Leu Asn Gln Glu Gly Ile Gln Arg Tyr 
225                 230                 235                 240 


Asn Leu Ala Leu Gly Gly Tyr Val Thr Lys Ser Gly Glu Lys Met Met 
                245                 250                 255     


Gly Leu Asn Asp Ala Leu Asn Leu Ala His Gln Ser Glu Lys Ser Ser 
            260                 265                 270         


Lys Gly Arg Ile His Met Thr Pro Leu Phe Lys Gln Ile Leu Ser Glu 
        275                 280                 285             


Lys Glu Ser Phe Ser Tyr Ile Pro Asp Val Phe Thr Glu Asp Ser Gln 
    290                 295                 300                 


Leu Leu Pro Ser Ile Gly Gly Phe Phe Ala Gln Ile Glu Asn Asp Lys 
305                 310                 315                 320 


Asp Gly Asn Ile Phe Asp Arg Ala Leu Glu Leu Ile Ser Ser Tyr Ala 
                325                 330                 335     


Glu Tyr Asp Thr Glu Arg Ile Tyr Ile Arg Gln Ala Asp Ile Asn Arg 
            340                 345                 350         


Val Ser Asn Val Ile Phe Gly Glu Trp Gly Thr Leu Gly Gly Leu Met 
        355                 360                 365             


Arg Glu Tyr Lys Ala Asp Ser Ile Asn Asp Ile Asn Leu Glu Arg Thr 
    370                 375                 380                 


Cys Lys Lys Val Asp Lys Trp Leu Asp Ser Lys Glu Phe Ala Leu Ser 
385                 390                 395                 400 


Asp Val Leu Glu Ala Ile Lys Arg Thr Gly Asn Asn Asp Ala Phe Asn 
                405                 410                 415     


Glu Tyr Ile Ser Lys Met Arg Thr Ala Arg Glu Lys Ile Asp Ala Ala 
            420                 425                 430         


Arg Lys Glu Met Lys Phe Ile Ser Glu Lys Ile Ser Gly Asp Glu Glu 
        435                 440                 445             


Ser Ile His Ile Ile Lys Thr Leu Leu Asp Ser Val Gln Gln Phe Leu 
    450                 455                 460                 


His Phe Phe Asn Leu Phe Lys Ala Arg Gln Asp Ile Pro Leu Asp Gly 
465                 470                 475                 480 


Ala Phe Tyr Ala Glu Phe Asp Glu Val His Ser Lys Leu Phe Ala Ile 
                485                 490                 495     


Val Pro Leu Tyr Asn Lys Val Arg Asn Tyr Leu Thr Lys Asn Asn Leu 
            500                 505                 510         


Asn Thr Lys Lys Ile Lys Leu Asn Phe Lys Asn Pro Thr Leu Ala Asn 
        515                 520                 525             


Gly Trp Asp Gln Asn Lys Val Tyr Asp Tyr Ala Ser Leu Ile Phe Leu 
    530                 535                 540                 


Arg Asp Gly Asn Tyr Tyr Leu Gly Ile Ile Asn Pro Lys Arg Lys Lys 
545                 550                 555                 560 


Asn Ile Lys Phe Glu Gln Gly Ser Gly Asn Gly Pro Phe Tyr Arg Lys 
                565                 570                 575     


Met Val Tyr Lys Gln Ile Pro Gly Pro Asn Lys Asn Leu Pro Arg Val 
            580                 585                 590         


Phe Leu Thr Ser Thr Lys Gly Lys Lys Glu Tyr Lys Pro Ser Lys Glu 
        595                 600                 605             


Ile Ile Glu Gly Tyr Glu Ala Asp Lys His Ile Arg Gly Asp Lys Phe 
    610                 615                 620                 


Asp Leu Asp Phe Cys His Lys Leu Ile Asp Phe Phe Lys Glu Ser Ile 
625                 630                 635                 640 


Glu Lys His Lys Asp Trp Ser Lys Phe Asn Phe Tyr Phe Ser Pro Thr 
                645                 650                 655     


Glu Ser Tyr Gly Asp Ile Ser Glu Phe Tyr Leu Asp Val Glu Lys Gln 
            660                 665                 670         


Gly Tyr Arg Met His Phe Glu Asn Ile Ser Ala Glu Thr Ile Asp Glu 
        675                 680                 685             


Tyr Val Glu Lys Gly Asp Leu Phe Leu Phe Gln Ile Tyr Asn Lys Asp 
    690                 695                 700                 


Phe Val Lys Ala Ala Thr Gly Lys Lys Asp Met His Thr Ile Tyr Trp 
705                 710                 715                 720 


Asn Ala Ala Phe Ser Pro Glu Asn Leu Gln Asp Val Val Val Lys Leu 
                725                 730                 735     


Asn Gly Glu Ala Glu Leu Phe Tyr Arg Asp Lys Ser Asp Ile Lys Glu 
            740                 745                 750         


Ile Val His Arg Glu Gly Glu Ile Leu Val Asn Arg Thr Tyr Asn Gly 
        755                 760                 765             


Arg Thr Pro Val Pro Asp Lys Ile His Lys Lys Leu Thr Asp Tyr His 
    770                 775                 780                 


Asn Gly Arg Thr Lys Asp Leu Gly Glu Ala Lys Glu Tyr Leu Asp Lys 
785                 790                 795                 800 


Val Arg Tyr Phe Lys Ala His Tyr Asp Ile Thr Lys Asp Arg Arg Tyr 
                805                 810                 815     


Leu Asn Asp Lys Ile Tyr Phe His Val Pro Leu Thr Leu Asn Phe Lys 
            820                 825                 830         


Ala Asn Gly Lys Lys Asn Leu Asn Lys Met Val Ile Glu Lys Phe Leu 
        835                 840                 845             


Ser Asp Glu Lys Ala His Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn 
    850                 855                 860                 


Leu Leu Tyr Tyr Ser Ile Ile Asp Arg Ser Gly Lys Ile Ile Asp Gln 
865                 870                 875                 880 


Gln Ser Leu Asn Val Ile Asp Gly Phe Asp Tyr Arg Glu Lys Leu Asn 
                885                 890                 895     


Gln Arg Glu Ile Glu Met Lys Asp Ala Arg Gln Ser Trp Asn Ala Ile 
            900                 905                 910         


Gly Lys Ile Lys Asp Leu Lys Glu Gly Tyr Leu Ser Lys Ala Val His 
        915                 920                 925             


Glu Ile Thr Lys Met Ala Ile Gln Tyr Asn Ala Ile Val Val Met Glu 
    930                 935                 940                 


Glu Leu Asn Tyr Gly Phe Lys Arg Gly Arg Phe Lys Val Glu Lys Gln 
945                 950                 955                 960 


Ile Tyr Gln Lys Phe Glu Asn Met Leu Ile Asp Lys Met Asn Tyr Leu 
                965                 970                 975     


Val Phe Lys Asp Ala Pro Asp Glu Ser Pro Gly Gly Val Leu Asn Ala 
            980                 985                 990         


Tyr Gln Leu Thr Asn Pro Leu Glu  Ser Phe Ala Lys Leu  Gly Lys Gln 
        995                 1000                 1005             


Thr Gly  Ile Leu Phe Tyr Val  Pro Ala Ala Tyr Thr  Ser Lys Ile 
    1010                 1015                 1020             


Asp Pro  Thr Thr Gly Phe Val  Asn Leu Phe Asn Thr  Ser Ser Lys 
    1025                 1030                 1035             


Thr Asn  Ala Gln Glu Arg Lys  Glu Phe Leu Gln Lys  Phe Glu Ser 
    1040                 1045                 1050             


Ile Ser  Tyr Ser Ala Lys Asp  Gly Gly Ile Phe Ala  Phe Ala Phe 
    1055                 1060                 1065             


Asp Tyr  Arg Lys Phe Gly Thr  Ser Lys Thr Asp His  Lys Asn Val 
    1070                 1075                 1080             


Trp Thr  Ala Tyr Thr Asn Gly  Glu Arg Met Arg Tyr  Ile Lys Glu 
    1085                 1090                 1095             


Lys Lys  Arg Asn Glu Leu Phe  Asp Pro Ser Lys Glu  Ile Lys Glu 
    1100                 1105                 1110             


Ala Leu  Thr Ser Ser Gly Ile  Lys Tyr Asp Gly Gly  Gln Asn Ile 
    1115                 1120                 1125             


Leu Pro  Asp Ile Leu Arg Ser  Asn Asn Asn Gly Leu  Ile Tyr Thr 
    1130                 1135                 1140             


Met Tyr  Ser Ser Phe Ile Ala  Ala Ile Gln Met Arg  Val Tyr Asp 
    1145                 1150                 1155             


Gly Lys  Glu Asp Tyr Ile Ile  Ser Pro Ile Lys Asn  Ser Lys Gly 
    1160                 1165                 1170             


Glu Phe  Phe Arg Thr Asp Pro  Lys Arg Arg Glu Leu  Pro Ile Asp 
    1175                 1180                 1185             


Ala Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala Leu Arg  Gly Glu Leu 
    1190                 1195                 1200             


Thr Met  Arg Ala Ile Ala Glu  Lys Phe Asp Pro Asp  Ser Glu Lys 
    1205                 1210                 1215             


Met Ala  Lys Leu Glu Leu Lys  His Lys Asp Trp Phe  Glu Phe Met 
    1220                 1225                 1230             


Gln Thr  Arg Gly Asp 
    1235             


<210>  44
<211>  1272
<212>  PRT
<213>  uncultured Clostridium sp.

<400>  44

Met Glu Asp Lys Gln Phe Leu Glu Arg Tyr Lys Glu Phe Ile Gly Leu 
1               5                   10                  15      


Asn Ser Leu Ser Lys Thr Leu Arg Asn Ser Leu Ile Pro Val Gly Ser 
            20                  25                  30          


Thr Leu Lys His Ile Gln Glu Tyr Gly Ile Leu Glu Glu Asp Ser Leu 
        35                  40                  45              


Arg Ala Gln Lys Arg Glu Glu Leu Lys Gly Ile Met Asp Asp Tyr Tyr 
    50                  55                  60                  


Arg Asn Tyr Ile Glu Met His Leu Arg Asp Val His Asp Ile Asp Trp 
65                  70                  75                  80  


Asn Glu Leu Phe Glu Ala Leu Thr Glu Val Lys Lys Asn Gln Thr Asp 
                85                  90                  95      


Asp Ala Lys Lys Cys Leu Glu Lys Ile Gln Glu Lys Lys Arg Lys Glu 
            100                 105                 110         


Ile Tyr Gln Tyr Leu Ser Asp Asp Ala Val Phe Ser Glu Met Phe Lys 
        115                 120                 125             


Glu Lys Met Ile Ser Gly Ile Leu Pro Asp Phe Ile Arg Cys Asn Glu 
    130                 135                 140                 


Glu Tyr Ser Glu Glu Glu Lys Glu Glu Lys Leu Lys Thr Val Ala Leu 
145                 150                 155                 160 


Phe His Arg Phe Thr Ser Ser Phe Asn Asp Phe Phe Leu Asn Arg Lys 
                165                 170                 175     


Asn Val Phe Thr Lys Glu Ala Ile Ala Thr Ala Ile Gly Tyr Arg Val 
            180                 185                 190         


Val His Glu Asn Ala Glu Ile Phe Leu Glu Asn Met Val Ala Phe Gln 
        195                 200                 205             


Asn Ile Gln Lys Ser Ala Glu Ser Gln Ile Ser Ile Ile Glu Arg Lys 
    210                 215                 220                 


Asn Glu His Tyr Phe Met Glu Trp Lys Leu Ser His Ile Phe Thr Ala 
225                 230                 235                 240 


Asp Tyr Tyr Met Met Leu Met Thr Gln Lys Ala Ile Glu His Tyr Asn 
                245                 250                 255     


Glu Met Cys Gly Val Val Asn Gln His Met Lys Glu Tyr Cys Gln Lys 
            260                 265                 270         


Glu Lys Lys Asn Trp Asn Leu Tyr Arg Met Lys Arg Leu His Lys Gln 
        275                 280                 285             


Ile Leu Ser Asn Ala Ser Thr Ser Phe Lys Ile Pro Glu Lys Tyr Glu 
    290                 295                 300                 


Asn Asp Ala Glu Val Tyr Glu Ser Val Asn Ser Phe Leu Gln Asn Val 
305                 310                 315                 320 


Met Glu Lys Thr Val Met Glu Arg Ile Ala Val Leu Lys Asn Asn Thr 
                325                 330                 335     


Asp Asn Phe Asp Leu Ser Lys Ile Tyr Ile Thr Ala Pro Tyr Tyr Glu 
            340                 345                 350         


Lys Ile Ser Asn Tyr Leu Cys Gly Ser Trp Asn Thr Ile Ala Asp Cys 
        355                 360                 365             


Leu Thr His Tyr Tyr Glu Gln Gln Ile Ala Gly Lys Gly Ala Arg Lys 
    370                 375                 380                 


Asp Gln Lys Val Lys Ala Ala Val Lys Ala Asp Lys Trp Lys Ser Leu 
385                 390                 395                 400 


Ser Glu Ile Glu Gln Leu Leu Lys Glu Tyr Ala Arg Ala Glu Glu Val 
                405                 410                 415     


Lys Arg Lys Pro Glu Glu Tyr Ile Ala Glu Ile Glu Asn Ile Val Ser 
            420                 425                 430         


Leu Lys Glu Val His Leu Leu Glu Tyr His Pro Glu Val Asn Leu Ile 
        435                 440                 445             


Glu Asn Glu Lys Tyr Ala Thr Glu Ile Lys Asp Val Leu Asp Asn Tyr 
    450                 455                 460                 


Met Glu Leu Phe His Trp Met Lys Trp Phe Tyr Ile Glu Glu Ala Val 
465                 470                 475                 480 


Glu Lys Glu Val Asn Phe Tyr Gly Glu Leu Asp Asp Leu Tyr Glu Glu 
                485                 490                 495     


Ile Arg Asp Ile Val Pro Leu Tyr Asn Lys Val Arg Asn Tyr Val Thr 
            500                 505                 510         


Gln Lys Pro Tyr Ser Asp Thr Lys Ile Lys Leu Asn Phe Gly Thr Pro 
        515                 520                 525             


Thr Leu Ala Asn Gly Trp Ser Lys Ser Lys Glu Tyr Asp Tyr Asn Ala 
    530                 535                 540                 


Ile Leu Leu Gln Lys Asp Gly Lys Tyr Tyr Met Gly Ile Phe Asn Pro 
545                 550                 555                 560 


Val Gln Lys Pro Glu Lys Glu Ile Ile Glu Gly His Ser His Pro Leu 
                565                 570                 575     


Glu Gly Asn Glu Tyr Lys Lys Met Val Tyr Tyr Tyr Leu Pro Ser Ala 
            580                 585                 590         


Asn Lys Met Leu Pro Lys Val Leu Leu Ser Lys Lys Gly Met Glu Ile 
        595                 600                 605             


Tyr Gln Pro Ser Glu Tyr Ile Ile Asn Gly Tyr Lys Glu Arg Arg His 
    610                 615                 620                 


Ile Lys Ser Glu Glu Lys Phe Asp Leu Gln Phe Cys His Asp Leu Ile 
625                 630                 635                 640 


Asp Tyr Phe Lys Ser Gly Ile Glu Arg Asn Pro Asp Trp Lys Val Phe 
                645                 650                 655     


Gly Phe His Phe Ser Asp Thr Asp Thr Tyr Gln Asp Ile Ser Gly Phe 
            660                 665                 670         


Tyr Arg Glu Val Glu Asp Gln Gly Tyr Lys Ile Asp Trp Thr Tyr Ile 
        675                 680                 685             


Lys Glu Ala Asp Ile Asp Arg Leu Asn Glu Glu Gly Lys Leu Tyr Leu 
    690                 695                 700                 


Phe Gln Ile Tyr Asn Lys Asp Phe Ser Glu Lys Ser Thr Gly Arg Glu 
705                 710                 715                 720 


Asn Leu His Thr Met Tyr Leu Lys Asn Leu Phe Ser Glu Glu Asn Ile 
                725                 730                 735     


Arg Glu Gln Val Leu Lys Leu Asn Gly Glu Ala Glu Ile Phe Phe Arg 
            740                 745                 750         


Lys Ser Ser Val Lys Lys Pro Ile Ile His Lys Lys Gly Thr Met Leu 
        755                 760                 765             


Val Asn Arg Thr Tyr Met Glu Glu Met His Gly Glu Ser Val Lys Lys 
    770                 775                 780                 


Asn Ile Pro Glu Lys Glu Tyr Gln Glu Ile Tyr Asn Tyr Met Asn His 
785                 790                 795                 800 


Arg Trp Lys Gly Glu Leu Ser Ala Glu Ala Lys Glu Tyr Leu Lys Lys 
                805                 810                 815     


Ala Val Cys His Glu Thr Lys Lys Asp Ile Val Lys Asp Tyr Arg Tyr 
            820                 825                 830         


Ser Val Asp Lys Phe Phe Ile His Leu Pro Ile Thr Ile Asn Tyr Arg 
        835                 840                 845             


Ala Ser Gly Lys Glu Ala Leu Asn Ser Val Ala Gln Arg Tyr Ile Ala 
    850                 855                 860                 


His Gln Asn Asp Met His Val Ile Gly Ile Asp Arg Gly Glu Arg Asn 
865                 870                 875                 880 


Leu Ile Tyr Val Ser Val Ile Asn Met Gln Gly Glu Ile Ile Glu Gln 
                885                 890                 895     


Lys Ser Phe Asn Val Val Asn Lys Tyr Asn Tyr Lys Glu Lys Leu Lys 
            900                 905                 910         


Glu Arg Glu Gln Asn Arg Asp Glu Ala Arg Lys Asn Trp Lys Glu Ile 
        915                 920                 925             


Gly Gln Ile Lys Asp Leu Lys Glu Gly Tyr Leu Ser Gly Val Ile His 
    930                 935                 940                 


Glu Ile Ala Lys Met Met Ile Lys Tyr His Ala Ile Val Ala Met Glu 
945                 950                 955                 960 


Asp Leu Asn Tyr Gly Phe Lys Arg Gly Arg Phe Lys Val Glu Arg Gln 
                965                 970                 975     


Val Tyr Gln Lys Phe Glu Asn Met Leu Ile Gln Lys Leu Asn Tyr Leu 
            980                 985                 990         


Val Phe Lys Asp Arg Ser Ala Asp  Glu Asp Gly Gly Val  Leu Arg Gly 
        995                 1000                 1005             


Tyr Gln  Leu Ala Tyr Ile Pro  Asp Ser Val Lys Lys  Leu Gly Arg 
    1010                 1015                 1020             


Gln Cys  Gly Met Ile Phe Tyr  Val Pro Ala Ala Phe  Thr Ser Lys 
    1025                 1030                 1035             


Ile Asp  Pro Ala Thr Gly Phe  Val Asp Ile Phe Asn  His Lys Ala 
    1040                 1045                 1050             


Tyr Thr  Thr Asp Gln Ala Lys  Arg Glu Phe Ile Leu  Ser Phe Asp 
    1055                 1060                 1065             


Glu Ile  Cys Tyr Asp Val Glu  Arg Gln Leu Phe Arg  Phe Thr Phe 
    1070                 1075                 1080             


Asp Tyr  Ala Asn Phe Ala Thr  His Asn Val Thr Leu  Ala Arg Asn 
    1085                 1090                 1095             


Asn Trp  Thr Ile Tyr Thr Asn  Gly Thr Arg Thr Gln  Lys Glu Phe 
    1100                 1105                 1110             


Val Asn  Arg Arg Val Arg Asp  Lys Lys Glu Val Phe  Asp Pro Thr 
    1115                 1120                 1125             


Glu Lys  Met Leu Lys Leu Leu  Glu Leu Glu Gly Val  Glu Tyr Gln 
    1130                 1135                 1140             


Ser Gly  Ala Asn Leu Leu Pro  Lys Leu Glu Lys Ile  Ser Asp Pro 
    1145                 1150                 1155             


His Leu  Phe His Glu Leu Gln  Arg Ile Val Arg Phe  Thr Val Gln 
    1160                 1165                 1170             


Leu Arg  Asn Ser Lys Asn Glu  Glu Asn Asp Val Asp  Tyr Asp His 
    1175                 1180                 1185             


Val Ile  Ser Pro Val Leu Asn  Glu Glu Gly Lys Phe  Phe Asp Ser 
    1190                 1195                 1200             


Ser Lys  Tyr Glu Asn Lys Glu  Glu Lys Lys Glu Ser  Leu Leu Pro 
    1205                 1210                 1215             


Val Asp  Ala Asp Ala Asn Gly  Ala Tyr Cys Ile Ala  Leu Lys Gly 
    1220                 1225                 1230             


Leu Tyr  Ile Met Gln Ala Ile  Gln Lys Asn Trp Ser  Glu Glu Lys 
    1235                 1240                 1245             


Ala Leu  Ser Pro Asp Val Leu  Arg Leu Asn Asn Asn  Asp Trp Phe 
    1250                 1255                 1260             


Asp Tyr  Ile Gln Asn Lys Arg  Tyr Arg 
    1265                 1270         


