                         SEQUENCE LISTING

<110>  TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LIMITED
       THE UNIVERSITY OF TOKYO
 
<120>  UBIQUITIN HIGH AFFINITY CYCLIC PEPTIDES AND METHODS OF USE 
       THEREOF

<130>  TECH-P-0151-PCT

<150>  US 62/681,389
<151>  2018-06-06

<150>  US 62/793,470
<151>  2019-01-17

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  1

Trp Tyr Asp Xaa Glu Tyr Tyr Tyr Leu Gly Tyr Gly Cys 
1               5                   10              


<210>  2
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (11)..(12)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  2

Gly Trp Phe Asp Asn Leu Tyr Trp Tyr Val Xaa Xaa Cys 
1               5                   10              


<210>  3
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is Leu, Gln, Thr, Asn, Glu, or Asp

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is Leu or Tyr

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X is Phe or Tyr

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is Val or Leu

<400>  3

Gly Trp Phe Asp Asp Xaa Tyr Xaa Xaa Xaa Ala Tyr Cys 
1               5                   10              


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  4

Leu Tyr Leu Asp Asp Xaa Gly Asp Trp Trp Ile Cys 
1               5                   10          


<210>  5
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X is Tyr Leu

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Orn

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is Tyr or His

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X is Gly or Ala

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is Val or Leu

<400>  5

Phe Gln Xaa Trp Xaa Xaa Ala Xaa Xaa Xaa Cys Gly 
1               5                   10          


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  6

Trp Trp Tyr Asp Xaa Glu Tyr Tyr Tyr Leu Gly Tyr Gly Cys 
1               5                   10                  


<210>  7
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (12)..(13)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  7

Trp Gly Trp Phe Asp Asn Leu Tyr Trp Tyr Val Xaa Xaa Cys 
1               5                   10                  


<210>  8
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X is Leu, Gln, Thr, Asn, Glu, or Asp

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X is Leu, or Tyr

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is Phe or Tyr

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  X is Val or Leu

<400>  8

Trp Gly Trp Phe Asp Asp Xaa Tyr Xaa Xaa Xaa Ala Tyr Cys 
1               5                   10                  


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  9

Trp Leu Tyr Leu Asp Asp Xaa Gly Asp Trp Trp Ile Cys 
1               5                   10              


<210>  10
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  10

Trp Tyr Asp Xaa Glu Tyr Tyr Tyr Leu Gly Tyr Gly Cys Gly 
1               5                   10                  


<210>  11
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (11)..(12)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  11

Gly Trp Phe Asp Asn Leu Tyr Trp Tyr Val Xaa Xaa Cys Gly 
1               5                   10                  


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is Leu, Gln, Thr, Asn, Glu, or Asp

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  X is Leu or Tyr

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  X is Phe or Tyr

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  X is Val or Leu

<400>  12

Gly Trp Phe Asp Asp Xaa Tyr Xaa Xaa Xaa Ala Tyr Cys Gly 
1               5                   10                  


<210>  13
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  X is any naturally occurring or non natrually occurring amino 
       acid

<400>  13

Leu Tyr Leu Asp Asp Xaa Gly Asp Trp Trp Ile Cys Gly 
1               5                   10              


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tryptophan is D-isomer Tryptophan

<400>  14

Trp Trp Tyr Asp Arg Glu Tyr Tyr Tyr Leu Gly Tyr Gly Cys Gly 
1               5                   10                  15  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tryptophan is D-isomer Tryptophan

<400>  15

Trp Gly Trp Phe Asp Asn Leu Tyr Trp Tyr Val Thr His Cys Gly 
1               5                   10                  15  


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tryptophan is D-isomer Tryptophan

<400>  16

Trp Gly Trp Phe Asp Asp Leu Tyr Leu Phe Val Ala Tyr Cys Gly 
1               5                   10                  15  


<210>  17
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Tryptophan is D-isomer Tryptophan

<400>  17

Trp Leu Tyr Leu Asp Asp Ser Gly Asp Trp Trp Ile Cys Gly 
1               5                   10                  


<210>  18
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18

Gly Trp Phe Asp Asp Gln Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


<210>  19
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19

Gly Trp Phe Asp Asp Thr Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


<210>  20
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20

Gly Trp Phe Asp Asp Asn Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


<210>  21
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21

Gly Trp Phe Asp Asp Glu Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


<210>  22
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22

Gly Trp Phe Asp Asp Asp Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


<210>  23
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23

Gly Trp Phe Asp Asp Leu Tyr Trp Phe Val Ala Tyr Cys 
1               5                   10              


<210>  24
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  24

Gly Trp Phe Asp Asp Leu Tyr Tyr Phe Val Ala Tyr Cys 
1               5                   10              


<210>  25
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  25

Gly Trp Phe Asp Asp Leu Tyr Leu Tyr Val Ala Tyr Cys 
1               5                   10              


<210>  26
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  26

Gly Trp Phe Asp Asp Leu Tyr Leu Phe Tyr Ala Tyr Cys 
1               5                   10              


<210>  27
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27

Gly Trp Phe Asp Asp Leu Tyr Leu Phe Leu Ala Tyr Cys 
1               5                   10              


<210>  28
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28

Gly Trp Phe Asp Asp Glu Tyr Trp Phe Tyr Ala Tyr Cys 
1               5                   10              


<210>  29
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29

Gly Trp Phe Asp Asp Leu Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  30
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  30

Gly Trp Phe Asp Asp Glu Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  31
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  31

Gly Trp Phe Asp Asp Gln Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  32
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  32

Gly Trp Phe Asp Asp Asn Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  33
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  33

Gly Trp Phe Asp Asp His Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  34
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  34

Gly Trp Phe Asp Asp Lys Tyr Trp Tyr Tyr Ala Tyr Cys 
1               5                   10              


<210>  35
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  35

Phe Gln Tyr Trp Xaa Tyr Ala Thr Gly Val Cys Gly 
1               5                   10          


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  36

Phe Gln Leu Trp Xaa His Ala Ile Ala Leu Cys Gly 
1               5                   10          


<210>  37
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)

<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Phenylalanine is D-isomer Phenylalanine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Orn

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  METHYLATION

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  METHYLATION

<400>  37

Phe Gln Tyr Trp Xaa Tyr Ala Thr Gly Val Cys Gly 
1               5                   10          


<210>  38
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Phenylalanine is D-isomer Phenylalanine

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Orn

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  METHYLATION

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  METHYLATION

<400>  38

Phe Gln Leu Trp Xaa His Ala Ile Ala Leu Cys Gly 
1               5                   10          


<210>  39
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  39
ggcgtaatac gactcactat aggatcgaaa gatttccgcg gccccgaaag gggattagcg       60

ttaggt                                                                  66


<210>  40
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  40
ggaucgaaag auuuccgcgg ccccgaaagg ggauuagcgu uaggu                       45


<210>  41
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  41
ggcgtaatac gactcactat aggcggggtg gagcagcctg gtagctcgtc gggctcataa       60

cccgaagatc gtcggttcaa atccggcccc cgcaacca                               98


<210>  42
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  42
ggcggggugg agcagccugg uagcucgucg ggcucauaac ccgaagaucg ucgguucaaa       60

uccggccccc gcaacca                                                      77


<210>  43
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  43
ctcccgcccc ccgtcc                                                       16


<210>  44
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  variation
<222>  (49)..(50)
<223>  n = equal c:g:a:t

<220>
<221>  repeat_unit
<222>  (49)..(51)
<223>  (nnk) repeats six (6) to twelve (12) times

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, t or u

<220>
<221>  variation
<222>  (51)..(51)
<223>  k = equal g:t

<400>  44
taatacgact cactataggg ttaactttaa gaaggagata tacatatgnn ktgcggcagc       60

ggcagcggca gctaggacgg ggggcggaaa                                        90


<210>  45
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  45

Gly Trp Phe Asp Asp Leu Tyr Leu Phe Val Ala Tyr Cys 
1               5                   10              


