                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
       Payne, Aimee  S.
       Ellebrecht, Christoph  T.
       Oh, Sangwook
       Milone, Michael  C.
 
<120>  COMPOSITIONS AND METHODS OF MUSCLE SPECIFIC KINASE  CHIMERIC 
       AUTOANTIBODY RECEPTOR CELLS

<130>  046483-7216WO1(02031)

<150>  62/680,769
<151>  2018-06-05

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  2058
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKWT.BBz CAAR

<400>  1
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccaa aagctcctgt catcaccact cctcttgaaa cagtggatgc cttagttgaa      120

gaagtggcta ctttcatgtg tgcagtggaa tcctaccccc agcctgagat ttcctggact      180

agaaataaaa ttctcattaa actctttgac acccggtaca gcatccggga gaatgggcag      240

ctcctcacca tcctgagtgt ggaagacagt gatgatggca tttactgctg cacggccaac      300

aatggtgtgg gaggagctgt ggagagttgt ggagccctgc aagtgaagat gaaacctaaa      360

ataactcgcc ctcccataaa tgtgaaaata atagagggat taaaagcagt cctaccatgt      420

actacaatgg gtaatcccaa accatcagtg tcttggataa agggagacag ccctctcagg      480

gaaaattccc gaattgcagt tcttgaatct gggagcttga ggattcataa cgtacaaaag      540

gaagatgcag gacagtatcg atgtgtggca aaaaacagcc tcgggacagc atattccaaa      600

gtggtgaagc tggaagttga ggtttttgcc aggattctgc gggctcctga atcccacaat      660

gtcacctttg gctcctttgt gaccctgcac tgtacagcaa caggcattcc tgtccccacc      720

atcacctgga ttgaaaacgg aaatgctgtt tcttctgggt ccattcaaga gagtgtgaaa      780

gaccgagtga ttgactcaag actgcagctg tttatcacca agccaggact ctacacatgc      840

atagctacca ataagcatgg ggagaagttc agtactgcca aggctgcagc caccatcagc      900

atagcagaat ggagtaaacc acagaaagat aacaaaggct actgcgccca gtacagaggg      960

gaggtgtgta atgcagtcct ggcaaaagat gctcttgttt ttctcaacac ctcctatgcg     1020

gaccctgagg aggcccaaga gctactggtc cacacggcct ggaatgaact gaaagtagtg     1080

agcccagtct gccggccagc tgctgaggct ttgttgtgta accacatctt ccaggagtgc     1140

agtcctggag tagtgcctac tcctattccc atttgcagag agtactgctt ggcagtaaag     1200

gagctcttct gcgcaaaaga atggctggta atggaagaga agacccacag aggactctac     1260

agatccgaga tgcatttgct gtccgtgcca gaatgcagca agcttcccag catgcattgg     1320

gaccccacgg cctgtgccag actgccacat ctagattata acaaagaaaa cctaaaaaca     1380

ttcccaccaa tgacgtcctc aaagccaagt gtggacattc caaatctgcc ttcctcctcc     1440

tcttcttcct tctctgtctc acctacatac tccatgactg ctagcggagg tggaggtagt     1500

ggcggtggag gcagctctgg tatctacatc tgggcaccct tggctggaac atgcggggtc     1560

ctgctgctga gcttggtgat caccctttac tgcaagcgcg gtcgcaagaa actgctctat     1620

atttttaaac agccattcat gagacctgtc cagaccactc aagaggagga cggatgttcc     1680

tgtagatttc ctgaagagga agaggggggg tgcgagctga gagtaaagtt cagtaggtcc     1740

gccgatgccc cagcctatca acaggggcaa aatcaactct acaacgaact taatctggga     1800

cgccgagagg agtacgatgt cttggataag agacgcggca gggaccctga aatgggcgga     1860

aagccaagac ggaagaaccc ccaggaaggt ctgtacaatg aacttcagaa agataagatg     1920

gccgaagcct acagcgagat cggcatgaaa ggagagaggc gccgcggcaa agggcatgat     1980

ggactgtatc agggtctcag tactgctact aaggacacat atgatgccct ccacatgcag     2040

gccctgccac caaggtga                                                   2058


<210>  2
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG Signal peptide

<400>  2
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgc          57


<210>  3
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MuSK extracellular domain

<400>  3
cttccaaaag ctcctgtcat caccactcct cttgaaacag tggatgcctt agttgaagaa       60

gtggctactt tcatgtgtgc agtggaatcc tacccccagc ctgagatttc ctggactaga      120

aataaaattc tcattaaact ctttgacacc cggtacagca tccgggagaa tgggcagctc      180

ctcaccatcc tgagtgtgga agacagtgat gatggcattt actgctgcac ggccaacaat      240

ggtgtgggag gagctgtgga gagttgtgga gccctgcaag tgaagatgaa acctaaaata      300

actcgccctc ccataaatgt gaaaataata gagggattaa aagcagtcct accatgtact      360

acaatgggta atcccaaacc atcagtgtct tggataaagg gagacagccc tctcagggaa      420

aattcccgaa ttgcagttct tgaatctggg agcttgagga ttcataacgt acaaaaggaa      480

gatgcaggac agtatcgatg tgtggcaaaa aacagcctcg ggacagcata ttccaaagtg      540

gtgaagctgg aagttgaggt ttttgccagg attctgcggg ctcctgaatc ccacaatgtc      600

acctttggct cctttgtgac cctgcactgt acagcaacag gcattcctgt ccccaccatc      660

acctggattg aaaacggaaa tgctgtttct tctgggtcca ttcaagagag tgtgaaagac      720

cgagtgattg actcaagact gcagctgttt atcaccaagc caggactcta cacatgcata      780

gctaccaata agcatgggga gaagttcagt actgccaagg ctgcagccac catcagcata      840

gcagaatgga gtaaaccaca gaaagataac aaaggctact gcgcccagta cagaggggag      900

gtgtgtaatg cagtcctggc aaaagatgct cttgtttttc tcaacacctc ctatgcggac      960

cctgaggagg cccaagagct actggtccac acggcctgga atgaactgaa agtagtgagc     1020

ccagtctgcc ggccagctgc tgaggctttg ttgtgtaacc acatcttcca ggagtgcagt     1080

cctggagtag tgcctactcc tattcccatt tgcagagagt actgcttggc agtaaaggag     1140

ctcttctgcg caaaagaatg gctggtaatg gaagagaaga cccacagagg actctacaga     1200

tccgagatgc atttgctgtc cgtgccagaa tgcagcaagc ttcccagcat gcattgggac     1260

cccacggcct gtgccagact gccacatcta gattataaca aagaaaacct aaaaacattc     1320

ccaccaatga cgtcctcaaa gccaagtgtg gacattccaa atctgccttc ctcctcctct     1380

tcttccttct ctgtctcacc tacatactcc atgact                               1416


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS Linker (codon optimized)

<400>  4
ggaggtggag gtagtggcgg tggaggcagc                                        30


<210>  5
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain (codon optimized)

<400>  5
atctacatct gggcaccctt ggctggaaca tgcggggtcc tgctgctgag cttggtgatc       60

accctttact gc                                                           72


<210>  6
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MuSK-I96A extracellular domain

<400>  6
cttccaaaag ctcctgtcat caccactcct cttgaaacag tggatgcctt agttgaagaa       60

gtggctactt tcatgtgtgc agtggaatcc tacccccagc ctgagatttc ctggactaga      120

aataaaattc tcattaaact ctttgacacc cggtacagca tccgggagaa tgggcagctc      180

ctcaccatcc tgagtgtgga agacagtgat gatggcgcat actgctgcac ggccaacaat      240

ggtgtgggag gagctgtgga gagttgtgga gccctgcaag tgaagatgaa acctaaaata      300

actcgccctc ccataaatgt gaaaataata gagggattaa aagcagtcct accatgtact      360

acaatgggta atcccaaacc atcagtgtct tggataaagg gagacagccc tctcagggaa      420

aattcccgaa ttgcagttct tgaatctggg agcttgagga ttcataacgt acaaaaggaa      480

gatgcaggac agtatcgatg tgtggcaaaa aacagcctcg ggacagcata ttccaaagtg      540

gtgaagctgg aagttgaggt ttttgccagg attctgcggg ctcctgaatc ccacaatgtc      600

acctttggct cctttgtgac cctgcactgt acagcaacag gcattcctgt ccccaccatc      660

acctggattg aaaacggaaa tgctgtttct tctgggtcca ttcaagagag tgtgaaagac      720

cgagtgattg actcaagact gcagctgttt atcaccaagc caggactcta cacatgcata      780

gctaccaata agcatgggga gaagttcagt actgccaagg ctgcagccac catcagcata      840

gcagaatgga gtaaaccaca gaaagataac aaaggctact gcgcccagta cagaggggag      900

gtgtgtaatg cagtcctggc aaaagatgct cttgtttttc tcaacacctc ctatgcggac      960

cctgaggagg cccaagagct actggtccac acggcctgga atgaactgaa agtagtgagc     1020

ccagtctgcc ggccagctgc tgaggctttg ttgtgtaacc acatcttcca ggagtgcagt     1080

cctggagtag tgcctactcc tattcccatt tgcagagagt actgcttggc agtaaaggag     1140

ctcttctgcg caaaagaatg gctggtaatg gaagagaaga cccacagagg actctacaga     1200

tccgagatgc atttgctgtc cgtgccagaa tgcagcaagc ttcccagcat gcattgggac     1260

cccacggcct gtgccagact gccacatcta gattataaca aagaaaacct aaaaacattc     1320

ccaccaatga cgtcctcaaa gccaagtgtg gacattccaa atctgccttc ctcctcctct     1380

tcttccttct ctgtctcacc tacatactcc atgact                               1416


<210>  7
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4-1BB intracellular domain (codon optimized)

<400>  7
aagcgcggtc gcaagaaact gctctatatt tttaaacagc cattcatgag acctgtccag       60

accactcaag aggaggacgg atgttcctgt agatttcctg aagaggaaga gggggggtgc      120

gagctg                                                                 126


<210>  8
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta intracellular domain (codon optimized)

<400>  8
agagtaaagt tcagtaggtc cgccgatgcc ccagcctatc aacaggggca aaatcaactc       60

tacaacgaac ttaatctggg acgccgagag gagtacgatg tcttggataa gagacgcggc      120

agggaccctg aaatgggcgg aaagccaaga cggaagaacc cccaggaagg tctgtacaat      180

gaacttcaga aagataagat ggccgaagcc tacagcgaga tcggcatgaa aggagagagg      240

cgccgcggca aagggcatga tggactgtat cagggtctca gtactgctac taaggacaca      300

tatgatgccc tccacatgca ggccctgcca ccaagg                                336


<210>  9
<211>  685
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKWT.BBz CAAR

<400>  9

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gly Ser Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu 
            20                  25                  30          


Glu Thr Val Asp Ala Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala 
        35                  40                  45              


Val Glu Ser Tyr Pro Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile 
    50                  55                  60                  


Leu Ile Lys Leu Phe Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln 
65                  70                  75                  80  


Leu Leu Thr Ile Leu Ser Val Glu Asp Ser Asp Asp Gly Ile Tyr Cys 
                85                  90                  95      


Cys Thr Ala Asn Asn Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala 
            100                 105                 110         


Leu Gln Val Lys Met Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val 
        115                 120                 125             


Lys Ile Ile Glu Gly Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly 
    130                 135                 140                 


Asn Pro Lys Pro Ser Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg 
145                 150                 155                 160 


Glu Asn Ser Arg Ile Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His 
                165                 170                 175     


Asn Val Gln Lys Glu Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn 
            180                 185                 190         


Ser Leu Gly Thr Ala Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val 
        195                 200                 205             


Phe Ala Arg Ile Leu Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly 
    210                 215                 220                 


Ser Phe Val Thr Leu His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr 
225                 230                 235                 240 


Ile Thr Trp Ile Glu Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln 
                245                 250                 255     


Glu Ser Val Lys Asp Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile 
            260                 265                 270         


Thr Lys Pro Gly Leu Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu 
        275                 280                 285             


Lys Phe Ser Thr Ala Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp 
    290                 295                 300                 


Ser Lys Pro Gln Lys Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly 
305                 310                 315                 320 


Glu Val Cys Asn Ala Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn 
                325                 330                 335     


Thr Ser Tyr Ala Asp Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr 
            340                 345                 350         


Ala Trp Asn Glu Leu Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala 
        355                 360                 365             


Glu Ala Leu Leu Cys Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val 
    370                 375                 380                 


Val Pro Thr Pro Ile Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys 
385                 390                 395                 400 


Glu Leu Phe Cys Ala Lys Glu Trp Leu Val Met Glu Glu Lys Thr His 
                405                 410                 415     


Arg Gly Leu Tyr Arg Ser Glu Met His Leu Leu Ser Val Pro Glu Cys 
            420                 425                 430         


Ser Lys Leu Pro Ser Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu 
        435                 440                 445             


Pro His Leu Asp Tyr Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met 
    450                 455                 460                 


Thr Ser Ser Lys Pro Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser 
465                 470                 475                 480 


Ser Ser Ser Phe Ser Val Ser Pro Thr Tyr Ser Met Thr Ala Ser Gly 
                485                 490                 495     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Ile Tyr Ile Trp Ala 
            500                 505                 510         


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
        515                 520                 525             


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
    530                 535                 540                 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
545                 550                 555                 560 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
                565                 570                 575     


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
            580                 585                 590         


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
        595                 600                 605             


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
    610                 615                 620                 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
625                 630                 635                 640 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
                645                 650                 655     


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            660                 665                 670         


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        675                 680                 685 


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG Signal peptide

<400>  10

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys 
            


<210>  11
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MuSK extracellular domain

<400>  11

Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu Glu Thr Val Asp Ala 
1               5                   10                  15      


Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala Val Glu Ser Tyr Pro 
            20                  25                  30          


Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile Leu Ile Lys Leu Phe 
        35                  40                  45              


Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln Leu Leu Thr Ile Leu 
    50                  55                  60                  


Ser Val Glu Asp Ser Asp Asp Gly Ile Tyr Cys Cys Thr Ala Asn Asn 
65                  70                  75                  80  


Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala Leu Gln Val Lys Met 
                85                  90                  95      


Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val Lys Ile Ile Glu Gly 
            100                 105                 110         


Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly Asn Pro Lys Pro Ser 
        115                 120                 125             


Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg Glu Asn Ser Arg Ile 
    130                 135                 140                 


Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His Asn Val Gln Lys Glu 
145                 150                 155                 160 


Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn Ser Leu Gly Thr Ala 
                165                 170                 175     


Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val Phe Ala Arg Ile Leu 
            180                 185                 190         


Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly Ser Phe Val Thr Leu 
        195                 200                 205             


His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr Ile Thr Trp Ile Glu 
    210                 215                 220                 


Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln Glu Ser Val Lys Asp 
225                 230                 235                 240 


Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile Thr Lys Pro Gly Leu 
                245                 250                 255     


Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu Lys Phe Ser Thr Ala 
            260                 265                 270         


Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp Ser Lys Pro Gln Lys 
        275                 280                 285             


Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly Glu Val Cys Asn Ala 
    290                 295                 300                 


Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn Thr Ser Tyr Ala Asp 
305                 310                 315                 320 


Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr Ala Trp Asn Glu Leu 
                325                 330                 335     


Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala Glu Ala Leu Leu Cys 
            340                 345                 350         


Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val Val Pro Thr Pro Ile 
        355                 360                 365             


Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys Glu Leu Phe Cys Ala 
    370                 375                 380                 


Lys Glu Trp Leu Val Met Glu Glu Lys Thr His Arg Gly Leu Tyr Arg 
385                 390                 395                 400 


Ser Glu Met His Leu Leu Ser Val Pro Glu Cys Ser Lys Leu Pro Ser 
                405                 410                 415     


Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu Pro His Leu Asp Tyr 
            420                 425                 430         


Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met Thr Ser Ser Lys Pro 
        435                 440                 445             


Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser Ser Ser Ser Phe Ser 
    450                 455                 460                 


Val Ser Pro Thr Tyr Ser Met Thr 
465                 470         


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  12

Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   


<210>  13
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  13

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  14
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB intracellular domain

<400>  14

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  15
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta intracellular domain

<400>  15

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  16
<211>  2058
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKWT.BBz CAAR

<400>  16
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccaa aagctcctgt catcaccact cctcttgaaa cagtggatgc cttagttgaa      120

gaagtggcta ctttcatgtg tgcagtggaa tcctaccccc agcctgagat ttcctggact      180

agaaataaaa ttctcattaa actctttgac acccggtaca gcatccggga gaatgggcag      240

ctcctcacca tcctgagtgt ggaagacagt gatgatggca tttactgctg cacggccaac      300

aatggtgtgg gaggagctgt ggagagttgt ggagccctgc aagtgaagat gaaacctaaa      360

ataactcgcc ctcccataaa tgtgaaaata atagagggat taaaagcagt cctaccatgt      420

actacaatgg gtaatcccaa accatcagtg tcttggataa agggagacag ccctctcagg      480

gaaaattccc gaattgcagt tcttgaatct gggagcttga ggattcataa cgtacaaaag      540

gaagatgcag gacagtatcg atgtgtggca aaaaacagcc tcgggacagc atattccaaa      600

gtggtgaagc tggaagttga ggtttttgcc aggattctgc gggctcctga atcccacaat      660

gtcacctttg gctcctttgt gaccctgcac tgtacagcaa caggcattcc tgtccccacc      720

atcacctgga ttgaaaacgg aaatgctgtt tcttctgggt ccattcaaga gagtgtgaaa      780

gaccgagtga ttgactcaag actgcagctg tttatcacca agccaggact ctacacatgc      840

atagctacca ataagcatgg ggagaagttc agtactgcca aggctgcagc caccatcagc      900

atagcagaat ggagtaaacc acagaaagat aacaaaggct actgcgccca gtacagaggg      960

gaggtgtgta atgcagtcct ggcaaaagat gctcttgttt ttctcaacac ctcctatgcg     1020

gaccctgagg aggcccaaga gctactggtc cacacggcct ggaatgaact gaaagtagtg     1080

agcccagtct gccggccagc tgctgaggct ttgttgtgta accacatctt ccaggagtgc     1140

agtcctggag tagtgcctac tcctattccc atttgcagag agtactgctt ggcagtaaag     1200

gagctcttct gcgcaaaaga atggctggta atggaagaga agacccacag aggactctac     1260

agatccgaga tgcatttgct gtccgtgcca gaatgcagca agcttcccag catgcattgg     1320

gaccccacgg cctgtgccag actgccacat ctagattata acaaagaaaa cctaaaaaca     1380

ttcccaccaa tgacgtcctc aaagccaagt gtggacattc caaatctgcc ttcctcctcc     1440

tcttcttcct tctctgtctc acctacatac tccatgactg ctagcggtgg cggaggttct     1500

ggaggtggag gttcctccgg aatctacatc tgggcgccct tggccgggac ttgtggggtc     1560

cttctcctgt cactggttat caccctttac tgcaaacggg gcagaaagaa actcctgtat     1620

atattcaaac aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc     1680

tgccgatttc cagaagaaga agaaggagga tgtgaactga gagtgaagtt cagcaggagc     1740

gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga     1800

cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga     1860

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg     1920

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat     1980

ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag     2040

gccctgcccc ctcgctaa                                                   2058


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GS Linker

<400>  17
ggtggcggag gttctggagg tggaggttcc                                        30


<210>  18
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  18
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc       60

accctttact gc                                                           72


<210>  19
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4-1BB intracellular domain

<400>  19
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  20
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3zeta intracellular domain

<400>  20
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  21
<211>  2058
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKI96A.BBz CAAR

<400>  21
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccaa aagctcctgt catcaccact cctcttgaaa cagtggatgc cttagttgaa      120

gaagtggcta ctttcatgtg tgcagtggaa tcctaccccc agcctgagat ttcctggact      180

agaaataaaa ttctcattaa actctttgac acccggtaca gcatccggga gaatgggcag      240

ctcctcacca tcctgagtgt ggaagacagt gatgatggcg catactgctg cacggccaac      300

aatggtgtgg gaggagctgt ggagagttgt ggagccctgc aagtgaagat gaaacctaaa      360

ataactcgcc ctcccataaa tgtgaaaata atagagggat taaaagcagt cctaccatgt      420

actacaatgg gtaatcccaa accatcagtg tcttggataa agggagacag ccctctcagg      480

gaaaattccc gaattgcagt tcttgaatct gggagcttga ggattcataa cgtacaaaag      540

gaagatgcag gacagtatcg atgtgtggca aaaaacagcc tcgggacagc atattccaaa      600

gtggtgaagc tggaagttga ggtttttgcc aggattctgc gggctcctga atcccacaat      660

gtcacctttg gctcctttgt gaccctgcac tgtacagcaa caggcattcc tgtccccacc      720

atcacctgga ttgaaaacgg aaatgctgtt tcttctgggt ccattcaaga gagtgtgaaa      780

gaccgagtga ttgactcaag actgcagctg tttatcacca agccaggact ctacacatgc      840

atagctacca ataagcatgg ggagaagttc agtactgcca aggctgcagc caccatcagc      900

atagcagaat ggagtaaacc acagaaagat aacaaaggct actgcgccca gtacagaggg      960

gaggtgtgta atgcagtcct ggcaaaagat gctcttgttt ttctcaacac ctcctatgcg     1020

gaccctgagg aggcccaaga gctactggtc cacacggcct ggaatgaact gaaagtagtg     1080

agcccagtct gccggccagc tgctgaggct ttgttgtgta accacatctt ccaggagtgc     1140

agtcctggag tagtgcctac tcctattccc atttgcagag agtactgctt ggcagtaaag     1200

gagctcttct gcgcaaaaga atggctggta atggaagaga agacccacag aggactctac     1260

agatccgaga tgcatttgct gtccgtgcca gaatgcagca agcttcccag catgcattgg     1320

gaccccacgg cctgtgccag actgccacat ctagattata acaaagaaaa cctaaaaaca     1380

ttcccaccaa tgacgtcctc aaagccaagt gtggacattc caaatctgcc ttcctcctcc     1440

tcttcttcct tctctgtctc acctacatac tccatgactg ctagcggagg tggaggtagt     1500

ggcggtggag gcagctctgg tatctacatc tgggcaccct tggctggaac atgcggggtc     1560

ctgctgctga gcttggtgat caccctttac tgcaagcgcg gtcgcaagaa actgctctat     1620

atttttaaac agccattcat gagacctgtc cagaccactc aagaggagga cggatgttcc     1680

tgtagatttc ctgaagagga agaggggggg tgcgagctga gagtaaagtt cagtaggtcc     1740

gccgatgccc cagcctatca acaggggcaa aatcaactct acaacgaact taatctggga     1800

cgccgagagg agtacgatgt cttggataag agacgcggca gggaccctga aatgggcgga     1860

aagccaagac ggaagaaccc ccaggaaggt ctgtacaatg aacttcagaa agataagatg     1920

gccgaagcct acagcgagat cggcatgaaa ggagagaggc gccgcggcaa agggcatgat     1980

ggactgtatc agggtctcag tactgctact aaggacacat atgatgccct ccacatgcag     2040

gccctgccac caaggtga                                                   2058


<210>  22
<211>  685
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKI96A.BBz CAAR

<400>  22

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gly Ser Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu 
            20                  25                  30          


Glu Thr Val Asp Ala Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala 
        35                  40                  45              


Val Glu Ser Tyr Pro Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile 
    50                  55                  60                  


Leu Ile Lys Leu Phe Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln 
65                  70                  75                  80  


Leu Leu Thr Ile Leu Ser Val Glu Asp Ser Asp Asp Gly Ala Tyr Cys 
                85                  90                  95      


Cys Thr Ala Asn Asn Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala 
            100                 105                 110         


Leu Gln Val Lys Met Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val 
        115                 120                 125             


Lys Ile Ile Glu Gly Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly 
    130                 135                 140                 


Asn Pro Lys Pro Ser Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg 
145                 150                 155                 160 


Glu Asn Ser Arg Ile Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His 
                165                 170                 175     


Asn Val Gln Lys Glu Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn 
            180                 185                 190         


Ser Leu Gly Thr Ala Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val 
        195                 200                 205             


Phe Ala Arg Ile Leu Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly 
    210                 215                 220                 


Ser Phe Val Thr Leu His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr 
225                 230                 235                 240 


Ile Thr Trp Ile Glu Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln 
                245                 250                 255     


Glu Ser Val Lys Asp Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile 
            260                 265                 270         


Thr Lys Pro Gly Leu Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu 
        275                 280                 285             


Lys Phe Ser Thr Ala Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp 
    290                 295                 300                 


Ser Lys Pro Gln Lys Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly 
305                 310                 315                 320 


Glu Val Cys Asn Ala Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn 
                325                 330                 335     


Thr Ser Tyr Ala Asp Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr 
            340                 345                 350         


Ala Trp Asn Glu Leu Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala 
        355                 360                 365             


Glu Ala Leu Leu Cys Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val 
    370                 375                 380                 


Val Pro Thr Pro Ile Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys 
385                 390                 395                 400 


Glu Leu Phe Cys Ala Lys Glu Trp Leu Val Met Glu Glu Lys Thr His 
                405                 410                 415     


Arg Gly Leu Tyr Arg Ser Glu Met His Leu Leu Ser Val Pro Glu Cys 
            420                 425                 430         


Ser Lys Leu Pro Ser Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu 
        435                 440                 445             


Pro His Leu Asp Tyr Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met 
    450                 455                 460                 


Thr Ser Ser Lys Pro Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser 
465                 470                 475                 480 


Ser Ser Ser Phe Ser Val Ser Pro Thr Tyr Ser Met Thr Ala Ser Gly 
                485                 490                 495     


Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Gly Ile Tyr Ile Trp Ala 
            500                 505                 510         


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
        515                 520                 525             


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
    530                 535                 540                 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
545                 550                 555                 560 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
                565                 570                 575     


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
            580                 585                 590         


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
        595                 600                 605             


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
    610                 615                 620                 


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
625                 630                 635                 640 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
                645                 650                 655     


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
            660                 665                 670         


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        675                 680                 685 


<210>  23
<211>  472
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MuSK-I96A extracellular domain

<400>  23

Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu Glu Thr Val Asp Ala 
1               5                   10                  15      


Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala Val Glu Ser Tyr Pro 
            20                  25                  30          


Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile Leu Ile Lys Leu Phe 
        35                  40                  45              


Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln Leu Leu Thr Ile Leu 
    50                  55                  60                  


Ser Val Glu Asp Ser Asp Asp Gly Ala Tyr Cys Cys Thr Ala Asn Asn 
65                  70                  75                  80  


Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala Leu Gln Val Lys Met 
                85                  90                  95      


Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val Lys Ile Ile Glu Gly 
            100                 105                 110         


Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly Asn Pro Lys Pro Ser 
        115                 120                 125             


Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg Glu Asn Ser Arg Ile 
    130                 135                 140                 


Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His Asn Val Gln Lys Glu 
145                 150                 155                 160 


Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn Ser Leu Gly Thr Ala 
                165                 170                 175     


Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val Phe Ala Arg Ile Leu 
            180                 185                 190         


Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly Ser Phe Val Thr Leu 
        195                 200                 205             


His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr Ile Thr Trp Ile Glu 
    210                 215                 220                 


Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln Glu Ser Val Lys Asp 
225                 230                 235                 240 


Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile Thr Lys Pro Gly Leu 
                245                 250                 255     


Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu Lys Phe Ser Thr Ala 
            260                 265                 270         


Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp Ser Lys Pro Gln Lys 
        275                 280                 285             


Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly Glu Val Cys Asn Ala 
    290                 295                 300                 


Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn Thr Ser Tyr Ala Asp 
305                 310                 315                 320 


Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr Ala Trp Asn Glu Leu 
                325                 330                 335     


Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala Glu Ala Leu Leu Cys 
            340                 345                 350         


Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val Val Pro Thr Pro Ile 
        355                 360                 365             


Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys Glu Leu Phe Cys Ala 
    370                 375                 380                 


Lys Glu Trp Leu Val Met Glu Glu Lys Thr His Arg Gly Leu Tyr Arg 
385                 390                 395                 400 


Ser Glu Met His Leu Leu Ser Val Pro Glu Cys Ser Lys Leu Pro Ser 
                405                 410                 415     


Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu Pro His Leu Asp Tyr 
            420                 425                 430         


Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met Thr Ser Ser Lys Pro 
        435                 440                 445             


Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser Ser Ser Ser Phe Ser 
    450                 455                 460                 


Val Ser Pro Thr Tyr Ser Met Thr 
465                 470         


<210>  24
<211>  2058
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKI96A.BBz CAAR

<400>  24
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccaa aagctcctgt catcaccact cctcttgaaa cagtggatgc cttagttgaa      120

gaagtggcta ctttcatgtg tgcagtggaa tcctaccccc agcctgagat ttcctggact      180

agaaataaaa ttctcattaa actctttgac acccggtaca gcatccggga gaatgggcag      240

ctcctcacca tcctgagtgt ggaagacagt gatgatggcg catactgctg cacggccaac      300

aatggtgtgg gaggagctgt ggagagttgt ggagccctgc aagtgaagat gaaacctaaa      360

ataactcgcc ctcccataaa tgtgaaaata atagagggat taaaagcagt cctaccatgt      420

actacaatgg gtaatcccaa accatcagtg tcttggataa agggagacag ccctctcagg      480

gaaaattccc gaattgcagt tcttgaatct gggagcttga ggattcataa cgtacaaaag      540

gaagatgcag gacagtatcg atgtgtggca aaaaacagcc tcgggacagc atattccaaa      600

gtggtgaagc tggaagttga ggtttttgcc aggattctgc gggctcctga atcccacaat      660

gtcacctttg gctcctttgt gaccctgcac tgtacagcaa caggcattcc tgtccccacc      720

atcacctgga ttgaaaacgg aaatgctgtt tcttctgggt ccattcaaga gagtgtgaaa      780

gaccgagtga ttgactcaag actgcagctg tttatcacca agccaggact ctacacatgc      840

atagctacca ataagcatgg ggagaagttc agtactgcca aggctgcagc caccatcagc      900

atagcagaat ggagtaaacc acagaaagat aacaaaggct actgcgccca gtacagaggg      960

gaggtgtgta atgcagtcct ggcaaaagat gctcttgttt ttctcaacac ctcctatgcg     1020

gaccctgagg aggcccaaga gctactggtc cacacggcct ggaatgaact gaaagtagtg     1080

agcccagtct gccggccagc tgctgaggct ttgttgtgta accacatctt ccaggagtgc     1140

agtcctggag tagtgcctac tcctattccc atttgcagag agtactgctt ggcagtaaag     1200

gagctcttct gcgcaaaaga atggctggta atggaagaga agacccacag aggactctac     1260

agatccgaga tgcatttgct gtccgtgcca gaatgcagca agcttcccag catgcattgg     1320

gaccccacgg cctgtgccag actgccacat ctagattata acaaagaaaa cctaaaaaca     1380

ttcccaccaa tgacgtcctc aaagccaagt gtggacattc caaatctgcc ttcctcctcc     1440

tcttcttcct tctctgtctc acctacatac tccatgactg ctagcggtgg cggaggttct     1500

ggaggtggag gttcctccgg aatctacatc tgggcgccct tggccgggac ttgtggggtc     1560

cttctcctgt cactggttat caccctttac tgcaaacggg gcagaaagaa actcctgtat     1620

atattcaaac aaccatttat gagaccagta caaactactc aagaggaaga tggctgtagc     1680

tgccgatttc cagaagaaga agaaggagga tgtgaactga gagtgaagtt cagcaggagc     1740

gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct caatctagga     1800

cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga gatgggggga     1860

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg     1920

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat     1980

ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct tcacatgcag     2040

gccctgcccc ctcgctaa                                                   2058


<210>  25
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKWT_opt.BBz CAAR

<400>  25
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccta aagccccggt aattaccacc ccattggaga ccgtcgatgc ccttgtagag      120

gaggttgcaa cctttatgtg tgctgtagag tcttacccgc aaccagagat atcatggacc      180

cgaaacaaga ttttgatcaa gttgttcgat actcgatact ccattcgaga gaacgggcag      240

ctcctcacta tattgagcgt agaagacagt gatgacggta tatactgctg caccgctaac      300

aatggtgtgg gaggagcagt ggaaagttgt ggcgcacttc aagtaaaaat gaagccgaaa      360

attacgagac ctccgattaa cgttaaaatt atagaggggc tgaaagctgt cctgccatgt      420

accacaatgg gtaatcccaa gcccagcgta tcctggatca aaggtgattc accgttgaga      480

gaaaattcta ggatagcggt attggagtcc ggctcactta gaattcacaa cgtccaaaaa      540

gaagatgctg gtcagtacag atgtgtcgcc aaaaattctc tcggaactgc atacagtaaa      600

gtggtaaagc ttgaagttga agtgtttgca aggattctgc gagccccgga gtcacacaat      660

gtaaccttcg gttcttttgt gactcttcat tgtaccgcta ctggaatccc agttcccacg      720

attacgtgga ttgaaaacgg aaatgccgtc tcaagcggca gcatacagga gtccgtgaag      780

gatagagtca tagactcccg attgcaactg ttcattacaa agcctggcct ttatacatgc      840

attgctacaa acaagcatgg tgagaaattc agtacagcta aggccgccgc aacaatttcc      900

attgcagagt ggagcaagcc acaaaaagat aacaagggtt actgtgccca atatcgaggg      960

gaagtttgta acgctgtact tgctaaggac gctctcgtct tcttgaatac atcctacgcg     1020

gacccggagg aagcccagga gctcttggtg cacactgcat ggaatgaact taaagtagtg     1080

tcccctgtat gccggccagc cgcggaagcg ttgctctgta atcacatttt ccaagaatgt     1140

tcaccagggg tagtaccaac gcctatcccg atatgtcggg aatattgtct ggcggtcaaa     1200

gagctctttt gtgctaaaga atggctcgtg atggaggaaa aaactcatcg gggtttgtat     1260

cgctcagaaa tgcacctgct gagtgtccca gaatgctcca agttgcccag tatgcactgg     1320

gaccctacgg cgtgcgcacg cttgcctcac ctggactaca ataaagaaaa tctgaaaaca     1380

tttcccccta tgactagcag taagccttct gttgatattc caaacctccc gtcatcctct     1440

tcatcttctt tctctgtcag cccgacttat tccatgactg gtggcggagg ttctggaggt     1500

ggaggttcct ccggaatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc     1560

ctgtcactgg ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc     1620

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga     1680

tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac     1740

gcccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga     1800

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg     1860

agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag     1920

gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt     1980

taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg     2040

ccccctcgct aa                                                         2052


<210>  26
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MuSK extracellular domain (codon optimized)

<400>  26
cttcctaaag ccccggtaat taccacccca ttggagaccg tcgatgccct tgtagaggag       60

gttgcaacct ttatgtgtgc tgtagagtct tacccgcaac cagagatatc atggacccga      120

aacaagattt tgatcaagtt gttcgatact cgatactcca ttcgagagaa cgggcagctc      180

ctcactatat tgagcgtaga agacagtgat gacggtatat actgctgcac cgctaacaat      240

ggtgtgggag gagcagtgga aagttgtggc gcacttcaag taaaaatgaa gccgaaaatt      300

acgagacctc cgattaacgt taaaattata gaggggctga aagctgtcct gccatgtacc      360

acaatgggta atcccaagcc cagcgtatcc tggatcaaag gtgattcacc gttgagagaa      420

aattctagga tagcggtatt ggagtccggc tcacttagaa ttcacaacgt ccaaaaagaa      480

gatgctggtc agtacagatg tgtcgccaaa aattctctcg gaactgcata cagtaaagtg      540

gtaaagcttg aagttgaagt gtttgcaagg attctgcgag ccccggagtc acacaatgta      600

accttcggtt cttttgtgac tcttcattgt accgctactg gaatcccagt tcccacgatt      660

acgtggattg aaaacggaaa tgccgtctca agcggcagca tacaggagtc cgtgaaggat      720

agagtcatag actcccgatt gcaactgttc attacaaagc ctggccttta tacatgcatt      780

gctacaaaca agcatggtga gaaattcagt acagctaagg ccgccgcaac aatttccatt      840

gcagagtgga gcaagccaca aaaagataac aagggttact gtgcccaata tcgaggggaa      900

gtttgtaacg ctgtacttgc taaggacgct ctcgtcttct tgaatacatc ctacgcggac      960

ccggaggaag cccaggagct cttggtgcac actgcatgga atgaacttaa agtagtgtcc     1020

cctgtatgcc ggccagccgc ggaagcgttg ctctgtaatc acattttcca agaatgttca     1080

ccaggggtag taccaacgcc tatcccgata tgtcgggaat attgtctggc ggtcaaagag     1140

ctcttttgtg ctaaagaatg gctcgtgatg gaggaaaaaa ctcatcgggg tttgtatcgc     1200

tcagaaatgc acctgctgag tgtcccagaa tgctccaagt tgcccagtat gcactgggac     1260

cctacggcgt gcgcacgctt gcctcacctg gactacaata aagaaaatct gaaaacattt     1320

ccccctatga ctagcagtaa gccttctgtt gatattccaa acctcccgtc atcctcttca     1380

tcttctttct ctgtcagccc gacttattcc atgact                               1416


<210>  27
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKWT_opt.BBz CAAR

<400>  27

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gly Ser Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu 
            20                  25                  30          


Glu Thr Val Asp Ala Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala 
        35                  40                  45              


Val Glu Ser Tyr Pro Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile 
    50                  55                  60                  


Leu Ile Lys Leu Phe Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln 
65                  70                  75                  80  


Leu Leu Thr Ile Leu Ser Val Glu Asp Ser Asp Asp Gly Ile Tyr Cys 
                85                  90                  95      


Cys Thr Ala Asn Asn Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala 
            100                 105                 110         


Leu Gln Val Lys Met Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val 
        115                 120                 125             


Lys Ile Ile Glu Gly Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly 
    130                 135                 140                 


Asn Pro Lys Pro Ser Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg 
145                 150                 155                 160 


Glu Asn Ser Arg Ile Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His 
                165                 170                 175     


Asn Val Gln Lys Glu Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn 
            180                 185                 190         


Ser Leu Gly Thr Ala Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val 
        195                 200                 205             


Phe Ala Arg Ile Leu Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly 
    210                 215                 220                 


Ser Phe Val Thr Leu His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr 
225                 230                 235                 240 


Ile Thr Trp Ile Glu Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln 
                245                 250                 255     


Glu Ser Val Lys Asp Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile 
            260                 265                 270         


Thr Lys Pro Gly Leu Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu 
        275                 280                 285             


Lys Phe Ser Thr Ala Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp 
    290                 295                 300                 


Ser Lys Pro Gln Lys Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly 
305                 310                 315                 320 


Glu Val Cys Asn Ala Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn 
                325                 330                 335     


Thr Ser Tyr Ala Asp Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr 
            340                 345                 350         


Ala Trp Asn Glu Leu Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala 
        355                 360                 365             


Glu Ala Leu Leu Cys Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val 
    370                 375                 380                 


Val Pro Thr Pro Ile Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys 
385                 390                 395                 400 


Glu Leu Phe Cys Ala Lys Glu Trp Leu Val Met Glu Glu Lys Thr His 
                405                 410                 415     


Arg Gly Leu Tyr Arg Ser Glu Met His Leu Leu Ser Val Pro Glu Cys 
            420                 425                 430         


Ser Lys Leu Pro Ser Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu 
        435                 440                 445             


Pro His Leu Asp Tyr Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met 
    450                 455                 460                 


Thr Ser Ser Lys Pro Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser 
465                 470                 475                 480 


Ser Ser Ser Phe Ser Val Ser Pro Thr Tyr Ser Met Thr Gly Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Gly Ser Ser Gly Ile Tyr Ile Trp Ala Pro Leu 
            500                 505                 510         


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
        515                 520                 525             


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    530                 535                 540                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
545                 550                 555                 560 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
                565                 570                 575     


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
            580                 585                 590         


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        595                 600                 605             


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
    610                 615                 620                 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
625                 630                 635                 640 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
                645                 650                 655     


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
            660                 665                 670         


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        675                 680             


<210>  28
<211>  2052
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKI96A_opt.BBz CAAR

<400>  28
atggagtttg ggctgagctg gctttttctt gtggctattt taaaaggtgt ccagtgcgga       60

tcccttccta aagccccggt aattaccacc ccattggaga ccgtcgatgc ccttgtagag      120

gaggttgcaa cctttatgtg tgctgtagag tcttacccgc aaccagagat atcatggacc      180

cgaaacaaga ttttgatcaa gttgttcgat actcgatact ccattcgaga gaacgggcag      240

ctcctcacta tattgagcgt agaagacagt gatgacggtg catactgctg caccgctaac      300

aatggtgtgg gaggagcagt ggaaagttgt ggcgcacttc aagtaaaaat gaagccgaaa      360

attacgagac ctccgattaa cgttaaaatt atagaggggc tgaaagctgt cctgccatgt      420

accacaatgg gtaatcccaa gcccagcgta tcctggatca aaggtgattc accgttgaga      480

gaaaattcta ggatagcggt attggagtcc ggctcactta gaattcacaa cgtccaaaaa      540

gaagatgctg gtcagtacag atgtgtcgcc aaaaattctc tcggaactgc atacagtaaa      600

gtggtaaagc ttgaagttga agtgtttgca aggattctgc gagccccgga gtcacacaat      660

gtaaccttcg gttcttttgt gactcttcat tgtaccgcta ctggaatccc agttcccacg      720

attacgtgga ttgaaaacgg aaatgccgtc tcaagcggca gcatacagga gtccgtgaag      780

gatagagtca tagactcccg attgcaactg ttcattacaa agcctggcct ttatacatgc      840

attgctacaa acaagcatgg tgagaaattc agtacagcta aggccgccgc aacaatttcc      900

attgcagagt ggagcaagcc acaaaaagat aacaagggtt actgtgccca atatcgaggg      960

gaagtttgta acgctgtact tgctaaggac gctctcgtct tcttgaatac atcctacgcg     1020

gacccggagg aagcccagga gctcttggtg cacactgcat ggaatgaact taaagtagtg     1080

tcccctgtat gccggccagc cgcggaagcg ttgctctgta atcacatttt ccaagaatgt     1140

tcaccagggg tagtaccaac gcctatcccg atatgtcggg aatattgtct ggcggtcaaa     1200

gagctctttt gtgctaaaga atggctcgtg atggaggaaa aaactcatcg gggtttgtat     1260

cgctcagaaa tgcacctgct gagtgtccca gaatgctcca agttgcccag tatgcactgg     1320

gaccctacgg cgtgcgcacg cttgcctcac ctggactaca ataaagaaaa tctgaaaaca     1380

tttcccccta tgactagcag taagccttct gttgatattc caaacctccc gtcatcctct     1440

tcatcttctt tctctgtcag cccgacttat tccatgactg gtggcggagg ttctggaggt     1500

ggaggttcct ccggaatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc     1560

ctgtcactgg ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc     1620

aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga     1680

tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac     1740

gcccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga     1800

gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg     1860

agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag     1920

gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt     1980

taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg     2040

ccccctcgct aa                                                         2052


<210>  29
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MuSK-I96A extracellular domain (codon optimized)

<400>  29
cttcctaaag ccccggtaat taccacccca ttggagaccg tcgatgccct tgtagaggag       60

gttgcaacct ttatgtgtgc tgtagagtct tacccgcaac cagagatatc atggacccga      120

aacaagattt tgatcaagtt gttcgatact cgatactcca ttcgagagaa cgggcagctc      180

ctcactatat tgagcgtaga agacagtgat gacggtgcat actgctgcac cgctaacaat      240

ggtgtgggag gagcagtgga aagttgtggc gcacttcaag taaaaatgaa gccgaaaatt      300

acgagacctc cgattaacgt taaaattata gaggggctga aagctgtcct gccatgtacc      360

acaatgggta atcccaagcc cagcgtatcc tggatcaaag gtgattcacc gttgagagaa      420

aattctagga tagcggtatt ggagtccggc tcacttagaa ttcacaacgt ccaaaaagaa      480

gatgctggtc agtacagatg tgtcgccaaa aattctctcg gaactgcata cagtaaagtg      540

gtaaagcttg aagttgaagt gtttgcaagg attctgcgag ccccggagtc acacaatgta      600

accttcggtt cttttgtgac tcttcattgt accgctactg gaatcccagt tcccacgatt      660

acgtggattg aaaacggaaa tgccgtctca agcggcagca tacaggagtc cgtgaaggat      720

agagtcatag actcccgatt gcaactgttc attacaaagc ctggccttta tacatgcatt      780

gctacaaaca agcatggtga gaaattcagt acagctaagg ccgccgcaac aatttccatt      840

gcagagtgga gcaagccaca aaaagataac aagggttact gtgcccaata tcgaggggaa      900

gtttgtaacg ctgtacttgc taaggacgct ctcgtcttct tgaatacatc ctacgcggac      960

ccggaggaag cccaggagct cttggtgcac actgcatgga atgaacttaa agtagtgtcc     1020

cctgtatgcc ggccagccgc ggaagcgttg ctctgtaatc acattttcca agaatgttca     1080

ccaggggtag taccaacgcc tatcccgata tgtcgggaat attgtctggc ggtcaaagag     1140

ctcttttgtg ctaaagaatg gctcgtgatg gaggaaaaaa ctcatcgggg tttgtatcgc     1200

tcagaaatgc acctgctgag tgtcccagaa tgctccaagt tgcccagtat gcactgggac     1260

cctacggcgt gcgcacgctt gcctcacctg gactacaata aagaaaatct gaaaacattt     1320

ccccctatga ctagcagtaa gccttctgtt gatattccaa acctcccgtc atcctcttca     1380

tcttctttct ctgtcagccc gacttattcc atgact                               1416


<210>  30
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pTRPE.MuSKI96A_opt.BBz CAAR

<400>  30

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gly Ser Leu Pro Lys Ala Pro Val Ile Thr Thr Pro Leu 
            20                  25                  30          


Glu Thr Val Asp Ala Leu Val Glu Glu Val Ala Thr Phe Met Cys Ala 
        35                  40                  45              


Val Glu Ser Tyr Pro Gln Pro Glu Ile Ser Trp Thr Arg Asn Lys Ile 
    50                  55                  60                  


Leu Ile Lys Leu Phe Asp Thr Arg Tyr Ser Ile Arg Glu Asn Gly Gln 
65                  70                  75                  80  


Leu Leu Thr Ile Leu Ser Val Glu Asp Ser Asp Asp Gly Ala Tyr Cys 
                85                  90                  95      


Cys Thr Ala Asn Asn Gly Val Gly Gly Ala Val Glu Ser Cys Gly Ala 
            100                 105                 110         


Leu Gln Val Lys Met Lys Pro Lys Ile Thr Arg Pro Pro Ile Asn Val 
        115                 120                 125             


Lys Ile Ile Glu Gly Leu Lys Ala Val Leu Pro Cys Thr Thr Met Gly 
    130                 135                 140                 


Asn Pro Lys Pro Ser Val Ser Trp Ile Lys Gly Asp Ser Pro Leu Arg 
145                 150                 155                 160 


Glu Asn Ser Arg Ile Ala Val Leu Glu Ser Gly Ser Leu Arg Ile His 
                165                 170                 175     


Asn Val Gln Lys Glu Asp Ala Gly Gln Tyr Arg Cys Val Ala Lys Asn 
            180                 185                 190         


Ser Leu Gly Thr Ala Tyr Ser Lys Val Val Lys Leu Glu Val Glu Val 
        195                 200                 205             


Phe Ala Arg Ile Leu Arg Ala Pro Glu Ser His Asn Val Thr Phe Gly 
    210                 215                 220                 


Ser Phe Val Thr Leu His Cys Thr Ala Thr Gly Ile Pro Val Pro Thr 
225                 230                 235                 240 


Ile Thr Trp Ile Glu Asn Gly Asn Ala Val Ser Ser Gly Ser Ile Gln 
                245                 250                 255     


Glu Ser Val Lys Asp Arg Val Ile Asp Ser Arg Leu Gln Leu Phe Ile 
            260                 265                 270         


Thr Lys Pro Gly Leu Tyr Thr Cys Ile Ala Thr Asn Lys His Gly Glu 
        275                 280                 285             


Lys Phe Ser Thr Ala Lys Ala Ala Ala Thr Ile Ser Ile Ala Glu Trp 
    290                 295                 300                 


Ser Lys Pro Gln Lys Asp Asn Lys Gly Tyr Cys Ala Gln Tyr Arg Gly 
305                 310                 315                 320 


Glu Val Cys Asn Ala Val Leu Ala Lys Asp Ala Leu Val Phe Leu Asn 
                325                 330                 335     


Thr Ser Tyr Ala Asp Pro Glu Glu Ala Gln Glu Leu Leu Val His Thr 
            340                 345                 350         


Ala Trp Asn Glu Leu Lys Val Val Ser Pro Val Cys Arg Pro Ala Ala 
        355                 360                 365             


Glu Ala Leu Leu Cys Asn His Ile Phe Gln Glu Cys Ser Pro Gly Val 
    370                 375                 380                 


Val Pro Thr Pro Ile Pro Ile Cys Arg Glu Tyr Cys Leu Ala Val Lys 
385                 390                 395                 400 


Glu Leu Phe Cys Ala Lys Glu Trp Leu Val Met Glu Glu Lys Thr His 
                405                 410                 415     


Arg Gly Leu Tyr Arg Ser Glu Met His Leu Leu Ser Val Pro Glu Cys 
            420                 425                 430         


Ser Lys Leu Pro Ser Met His Trp Asp Pro Thr Ala Cys Ala Arg Leu 
        435                 440                 445             


Pro His Leu Asp Tyr Asn Lys Glu Asn Leu Lys Thr Phe Pro Pro Met 
    450                 455                 460                 


Thr Ser Ser Lys Pro Ser Val Asp Ile Pro Asn Leu Pro Ser Ser Ser 
465                 470                 475                 480 


Ser Ser Ser Phe Ser Val Ser Pro Thr Tyr Ser Met Thr Gly Gly Gly 
                485                 490                 495     


Gly Ser Gly Gly Gly Gly Ser Ser Gly Ile Tyr Ile Trp Ala Pro Leu 
            500                 505                 510         


Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr 
        515                 520                 525             


Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    530                 535                 540                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
545                 550                 555                 560 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
                565                 570                 575     


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
            580                 585                 590         


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
        595                 600                 605             


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
    610                 615                 620                 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
625                 630                 635                 640 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
                645                 650                 655     


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
            660                 665                 670         


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        675                 680             


<210>  31
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 alpha hinge

<400>  31

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp 
    50                  55  


<210>  32
<211>  165
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 alpha hinge

<400>  32
ttcgtgccgg tcttcctgcc agcgaagcca accacgacgc cagcaccgcg accaccaaca       60

cctgcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg cagaccagca      120

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgat                      165


