                         SEQUENCE LISTING

<110>  A2 Biotherapeutics, Inc.
       JOHNSTON, James
       DARIS, Mark
       CUI, Jiajia
       MARTIN, Aaron D.
 
<120>  FOXP3-EXPRESSING CAR-T REGULATORY CELLS

<130>  A2BI-003/01WO 331656-2007

<150>  62/685,562
<151>  2018-06-15

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  1794
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.05 CAR-T v2 CD28

<400>  1
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc agggactcag tacagccacc aaggacacct acgacgccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgtg gctgcagagc ctgctgctct tgggcactgt ggcctgcagc      780

atctctcgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      840

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      900

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      960

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct     1020

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag     1080

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc     1140

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat     1200

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata     1260

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc     1320

cccgagggct gctggggccc ggagcccagg gactgcgtgt cttgccggaa tgtcagccga     1380

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag     1440

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc     1500

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc     1560

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca     1620

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca     1680

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1740

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtag           1794


<210>  2
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.12 CAR-T v2 CD28 CD3z FoxP3 wt

<400>  2
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc agggactcag tacagccacc aaggacacct acgacgccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgcc caaccccagg cctggcaagc cctcggcccc ttccttggcc      780

cttggcccat ccccaggagc ctcgcccagc tggagggctg cacccaaagc ctcagacctg      840

ctgggggccc ggggcccagg gggaaccttc cagggccgag atcttcgagg cggggcccat      900

gcctcctctt cttccttgaa ccccatgcca ccatcgcagc tgcagctgcc cacactgccc      960

ctagtcatgg tggcaccctc cggggcacgg ctgggcccct tgccccactt acaggcactc     1020

ctccaggaca ggccacattt catgcaccag ctctcaacgg tggatgccca cgcccggacc     1080

cctgtgctgc aggtgcaccc cctggagagc ccagccatga tcagcctcac accacccacc     1140

accgccactg gggtcttctc cctcaaggcc cggcctggcc tcccacctgg gatcaacgtg     1200

gccagcctgg aatgggtgtc cagggagccg gcactgctct gcaccttccc aaatcccagt     1260

gcacccagga aggacagcac cctttcggct gtgccccaga gctcctaccc actgctggca     1320

aatggtgtct gcaagtggcc cggatgtgag aaggtcttcg aagagccaga ggacttcctc     1380

aagcactgcc aggcggacca tcttctggat gagaagggca gggcacaatg tctcctccag     1440

agagagatgg tacagtctct ggagcagcag ctggtgctgg agaaggagaa gctgagtgcc     1500

atgcaggccc acctggctgg gaaaatggca ctgaccaagg cttcatctgt ggcatcatcc     1560

gacaagggct cctgctgcat cgtagctgct ggcagccaag gccctgtcgt cccagcctgg     1620

tctggccccc gggaggcccc tgacagcctg tttgctgtcc ggaggcacct gtggggtagc     1680

catggaaaca gcacattccc agagttcctc cacaacatgg actacttcaa gttccacaac     1740

atgcgacccc ctttcaccta cgccacgctc atccgctggg ccatcctgga ggctccagag     1800

aagcagcgga cactcaatga gatctaccac tggttcacac gcatgtttgc cttcttcaga     1860

aaccatcctg ccacctggaa gaacgccatc cgccacaacc tgagtctgca caagtgcttt     1920

gtgcgggtgg agagcgagaa gggggctgtg tggaccgtgg atgagctgga gttccgcaag     1980

aaacggagcc agaggcccag caggtgttcc aaccctacac ctggccccta g              2031


<210>  3
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.13 CAR-T v2 CD28 CD3z FoxP3 S418E

<400>  3
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc agggactcag tacagccacc aaggacacct acgacgccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgcc caaccccagg cctggcaagc cctcggcccc ttccttggcc      780

cttggcccat ccccaggagc ctcgcccagc tggagggctg cacccaaagc ctcagacctg      840

ctgggggccc ggggcccagg gggaaccttc cagggccgag atcttcgagg cggggcccat      900

gcctcctctt cttccttgaa ccccatgcca ccatcgcagc tgcagctgcc cacactgccc      960

ctagtcatgg tggcaccctc cggggcacgg ctgggcccct tgccccactt acaggcactc     1020

ctccaggaca ggccacattt catgcaccag ctctcaacgg tggatgccca cgcccggacc     1080

cctgtgctgc aggtgcaccc cctggagagc ccagccatga tcagcctcac accacccacc     1140

accgccactg gggtcttctc cctcaaggcc cggcctggcc tcccacctgg gatcaacgtg     1200

gccagcctgg aatgggtgtc cagggagccg gcactgctct gcaccttccc aaatcccagt     1260

gcacccagga aggacagcac cctttcggct gtgccccaga gctcctaccc actgctggca     1320

aatggtgtct gcaagtggcc cggatgtgag aaggtcttcg aagagccaga ggacttcctc     1380

aagcactgcc aggcggacca tcttctggat gagaagggca gggcacaatg tctcctccag     1440

agagagatgg tacagtctct ggagcagcag ctggtgctgg agaaggagaa gctgagtgcc     1500

atgcaggccc acctggctgg gaaaatggca ctgaccaagg cttcatctgt ggcatcatcc     1560

gacaagggct cctgctgcat cgtagctgct ggcagccaag gccctgtcgt cccagcctgg     1620

tctggccccc gggaggcccc tgacagcctg tttgctgtcc ggaggcacct gtggggtagc     1680

catggaaaca gcacattccc agagttcctc cacaacatgg actacttcaa gttccacaac     1740

atgcgacccc ctttcaccta cgccacgctc atccgctggg ccatcctgga ggctccagag     1800

aagcagcgga cactcaatga gatctaccac tggttcacac gcatgtttgc cttcttcaga     1860

aaccatcctg ccacctggaa gaacgccatc cgccacaacc tgagtctgca caagtgcttt     1920

gtgcgggtgg agagcgagaa gggggctgtg tggaccgtgg atgagctgga gttccgcaag     1980

aaacgggagc agaggcccag caggtgttcc aaccctacac ctggccccta g              2031


<210>  4
<211>  1839
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.14 CAR-T v2 CD28 CD3z FoxP3 NC

<400>  4
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc agggactcag tacagccacc aaggacacct acgacgccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatggg cggggcccat gcctcctctt cttccttgaa ccccatgcca      780

ccatcgcagc tgcagctgcc cacactgccc ctagtcatgg tggcaccctc cggggcacgg      840

ctgggcccct tgccccactt acaggcactc ctccaggaca ggccacattt catgcaccag      900

ctctcaacgg tggatgccca cgcccggacc cctgtgctgc aggtgcaccc cctggagagc      960

ccagccatga tcagcctcac accacccacc accgccactg gggtcttctc cctcaaggcc     1020

cggcctggcc tcccacctgg gatcaacgtg gccagcctgg aatgggtgtc cagggagccg     1080

gcactgctct gcaccttccc aaatcccagt gcacccagga aggacagcac cctttcggct     1140

gtgccccaga gctcctaccc actgctggca aatggtgtct gcaagtggcc cggatgtgag     1200

aaggtcttcg aagagccaga ggacttcctc aagcactgcc aggcggacca tcttctggat     1260

gagaagggca gggcacaatg tctcctccag agagagatgg tacagtctct ggagcagcag     1320

ctggtgctgg agaaggagaa gctgagtgcc atgcaggccc acctggctgg gaaaatggca     1380

ctgaccaagg cttcatctgt ggcatcatcc gacaagggct cctgctgcat cgtagctgct     1440

ggcagccaag gccctgtcgt cccagcctgg tctggccccc gggaggcccc tgacagcctg     1500

tttgctgtcc ggaggcacct gtggggtagc catggaaaca gcacattccc agagttcctc     1560

cacaacatgg actacttcaa gttccacaac atgcgacccc ctttcaccta cgccacgctc     1620

atccgctggg ccatcctgga ggctccagag aagcagcgga cactcaatga gatctaccac     1680

tggttcacac gcatgtttgc cttcttcaga aaccatcctg ccacctggaa gaacgccatc     1740

cgccacaacc tgagtctgca caagtgcttt gtgcgggtgg agagcgagaa gggggctgtg     1800

tggaccgtgg atgagctgga gttccgcaag aaacggtag                            1839


<210>  5
<211>  1794
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.15 CAR-T v2 CD28 CD3z STAT5 2x

<400>  5
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc tgtcactcag tacagccacc aaggacacct acctctccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgtg gctgcagagc ctgctgctct tgggcactgt ggcctgcagc      780

atctctcgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata      840

aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc      900

ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa      960

ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct     1020

gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag     1080

caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc     1140

tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat     1200

gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata     1260

agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc     1320

cccgagggct gctggggccc ggagcccagg gactgcgtgt cttgccggaa tgtcagccga     1380

ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag     1440

aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc     1500

acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc     1560

gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca     1620

gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca     1680

ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg     1740

ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gtag           1794


<210>  6
<211>  2031
<212>  DNA
<213>  Artificial sequence

<220>
<223>  GG.16 CAR-T v2 CD28 CD3z STAT5 2x FoxP3 wt

<400>  6
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc tgtcactcag tacagccacc aaggacacct acctctccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgcc caaccccagg cctggcaagc cctcggcccc ttccttggcc      780

cttggcccat ccccaggagc ctcgcccagc tggagggctg cacccaaagc ctcagacctg      840

ctgggggccc ggggcccagg gggaaccttc cagggccgag atcttcgagg cggggcccat      900

gcctcctctt cttccttgaa ccccatgcca ccatcgcagc tgcagctgcc cacactgccc      960

ctagtcatgg tggcaccctc cggggcacgg ctgggcccct tgccccactt acaggcactc     1020

ctccaggaca ggccacattt catgcaccag ctctcaacgg tggatgccca cgcccggacc     1080

cctgtgctgc aggtgcaccc cctggagagc ccagccatga tcagcctcac accacccacc     1140

accgccactg gggtcttctc cctcaaggcc cggcctggcc tcccacctgg gatcaacgtg     1200

gccagcctgg aatgggtgtc cagggagccg gcactgctct gcaccttccc aaatcccagt     1260

gcacccagga aggacagcac cctttcggct gtgccccaga gctcctaccc actgctggca     1320

aatggtgtct gcaagtggcc cggatgtgag aaggtcttcg aagagccaga ggacttcctc     1380

aagcactgcc aggcggacca tcttctggat gagaagggca gggcacaatg tctcctccag     1440

agagagatgg tacagtctct ggagcagcag ctggtgctgg agaaggagaa gctgagtgcc     1500

atgcaggccc acctggctgg gaaaatggca ctgaccaagg cttcatctgt ggcatcatcc     1560

gacaagggct cctgctgcat cgtagctgct ggcagccaag gccctgtcgt cccagcctgg     1620

tctggccccc gggaggcccc tgacagcctg tttgctgtcc ggaggcacct gtggggtagc     1680

catggaaaca gcacattccc agagttcctc cacaacatgg actacttcaa gttccacaac     1740

atgcgacccc ctttcaccta cgccacgctc atccgctggg ccatcctgga ggctccagag     1800

aagcagcgga cactcaatga gatctaccac tggttcacac gcatgtttgc cttcttcaga     1860

aaccatcctg ccacctggaa gaacgccatc cgccacaacc tgagtctgca caagtgcttt     1920

gtgcgggtgg agagcgagaa gggggctgtg tggaccgtgg atgagctgga gttccgcaag     1980

aaacggagcc agaggcccag caggtgttcc aaccctacac ctggccccta g              2031


<210>  7
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.17 CAR-T v2 CD28 CD3z STAT5 2x FoxP3 S418E

<400>  7
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc tgtcactcag tacagccacc aaggacacct acctctccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatgcc caaccccagg cctggcaagc cctcggcccc ttccttggcc      780

cttggcccat ccccaggagc ctcgcccagc tggagggctg cacccaaagc ctcagacctg      840

ctgggggccc ggggcccagg gggaaccttc cagggccgag atcttcgagg cggggcccat      900

gcctcctctt cttccttgaa ccccatgcca ccatcgcagc tgcagctgcc cacactgccc      960

ctagtcatgg tggcaccctc cggggcacgg ctgggcccct tgccccactt acaggcactc     1020

ctccaggaca ggccacattt catgcaccag ctctcaacgg tggatgccca cgcccggacc     1080

cctgtgctgc aggtgcaccc cctggagagc ccagccatga tcagcctcac accacccacc     1140

accgccactg gggtcttctc cctcaaggcc cggcctggcc tcccacctgg gatcaacgtg     1200

gccagcctgg aatgggtgtc cagggagccg gcactgctct gcaccttccc aaatcccagt     1260

gcacccagga aggacagcac cctttcggct gtgccccaga gctcctaccc actgctggca     1320

aatggtgtct gcaagtggcc cggatgtgag aaggtcttcg aagagccaga ggacttcctc     1380

aagcactgcc aggcggacca tcttctggat gagaagggca gggcacaatg tctcctccag     1440

agagagatgg tacagtctct ggagcagcag ctggtgctgg agaaggagaa gctgagtgcc     1500

atgcaggccc acctggctgg gaaaatggca ctgaccaagg cttcatctgt ggcatcatcc     1560

gacaagggct cctgctgcat cgtagctgct ggcagccaag gccctgtcgt cccagcctgg     1620

tctggccccc gggaggcccc tgacagcctg tttgctgtcc ggaggcacct gtggggtagc     1680

catggaaaca gcacattccc agagttcctc cacaacatgg actacttcaa gttccacaac     1740

atgcgacccc ctttcaccta cgccacgctc atccgctggg ccatcctgga ggctccagag     1800

aagcagcgga cactcaatga gatctaccac tggttcacac gcatgtttgc cttcttcaga     1860

aaccatcctg ccacctggaa gaacgccatc cgccacaacc tgagtctgca caagtgcttt     1920

gtgcgggtgg agagcgagaa gggggctgtg tggaccgtgg atgagctgga gttccgcaag     1980

aaacgggagc agaggcccag caggtgttcc aaccctacac ctggccccta g              2031


<210>  8
<211>  1839
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GG.18 CAR-T v2 CD28 CD3z STAT5 2x FoxP3 NC

<400>  8
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatttctg ggtgctggtc gttgtgggcg gcgtgctggc ctgctacagc      180

ctgctggtga cagtggcctt catcatcttt tgggtgagga gcaagcggag cagactgctg      240

cacagcgact acatgaacat gaccccccgg aggcctggcc ccacccggaa gcactaccag      300

ccctacgccc ctcccaggga tttcgccgcc taccggagca gagtgaagtt cagcaggagc      360

gcagacgccc ccgcgtacaa gcagggccag aaccagctct ataacgagct caatctagga      420

cgaagagagg agtacgatgt tttggacaag cgtagaggcc gggaccctga gatgggggga      480

aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg      540

gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa ggggcacgat      600

ggcctttacc tgtcactcag tacagccacc aaggacacct acctctccct tcacatgcag      660

gccctgcccc ctcgccccgg ggagggcaga ggcagcctgc tgacatgtgg cgacgtggaa      720

gagaaccctg gccccatggg cggggcccat gcctcctctt cttccttgaa ccccatgcca      780

ccatcgcagc tgcagctgcc cacactgccc ctagtcatgg tggcaccctc cggggcacgg      840

ctgggcccct tgccccactt acaggcactc ctccaggaca ggccacattt catgcaccag      900

ctctcaacgg tggatgccca cgcccggacc cctgtgctgc aggtgcaccc cctggagagc      960

ccagccatga tcagcctcac accacccacc accgccactg gggtcttctc cctcaaggcc     1020

cggcctggcc tcccacctgg gatcaacgtg gccagcctgg aatgggtgtc cagggagccg     1080

gcactgctct gcaccttccc aaatcccagt gcacccagga aggacagcac cctttcggct     1140

gtgccccaga gctcctaccc actgctggca aatggtgtct gcaagtggcc cggatgtgag     1200

aaggtcttcg aagagccaga ggacttcctc aagcactgcc aggcggacca tcttctggat     1260

gagaagggca gggcacaatg tctcctccag agagagatgg tacagtctct ggagcagcag     1320

ctggtgctgg agaaggagaa gctgagtgcc atgcaggccc acctggctgg gaaaatggca     1380

ctgaccaagg cttcatctgt ggcatcatcc gacaagggct cctgctgcat cgtagctgct     1440

ggcagccaag gccctgtcgt cccagcctgg tctggccccc gggaggcccc tgacagcctg     1500

tttgctgtcc ggaggcacct gtggggtagc catggaaaca gcacattccc agagttcctc     1560

cacaacatgg actacttcaa gttccacaac atgcgacccc ctttcaccta cgccacgctc     1620

atccgctggg ccatcctgga ggctccagag aagcagcgga cactcaatga gatctaccac     1680

tggttcacac gcatgtttgc cttcttcaga aaccatcctg ccacctggaa gaacgccatc     1740

cgccacaacc tgagtctgca caagtgcttt gtgcgggtgg agagcgagaa gggggctgtg     1800

tggaccgtgg atgagctgga gttccgcaag aaacggtag                            1839


<210>  9
<211>  431
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met 
                245                 250                 255     


Gln Ala His Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val 
            260                 265                 270         


Ala Ser Ser Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln 
        275                 280                 285             


Gly Pro Val Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser 
    290                 295                 300                 


Leu Phe Ala Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr 
305                 310                 315                 320 


Phe Pro Glu Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met 
                325                 330                 335     


Arg Pro Pro Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu 
            340                 345                 350         


Ala Pro Glu Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr 
        355                 360                 365             


Arg Met Phe Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala 
    370                 375                 380                 


Ile Arg His Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser 
385                 390                 395                 400 


Glu Lys Gly Ala Val Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys 
                405                 410                 415     


Arg Ser Gln Arg Pro Ser Arg Cys Ser Asn Pro Thr Pro Gly Pro 
            420                 425                 430     


<210>  10
<211>  362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  minimal Foxp3

<400>  10

Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met Pro Pro Ser 
1               5                   10                  15      


Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala Pro Ser Gly 
            20                  25                  30          


Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu Gln Asp Arg 
        35                  40                  45              


Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His Ala Arg Thr 
    50                  55                  60                  


Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met Ile Ser Leu 
65                  70                  75                  80  


Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys Ala Arg Pro 
                85                  90                  95      


Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp Val Ser Arg 
            100                 105                 110         


Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala Pro Arg Lys 
        115                 120                 125             


Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro Leu Leu Ala 
    130                 135                 140                 


Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe Glu Glu Pro 
145                 150                 155                 160 


Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu Asp Glu Lys 
                165                 170                 175     


Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln Ser Leu Glu 
            180                 185                 190         


Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met Gln Ala His 
        195                 200                 205             


Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val Ala Ser Ser 
    210                 215                 220                 


Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln Gly Pro Val 
225                 230                 235                 240 


Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser Leu Phe Ala 
                245                 250                 255     


Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr Phe Pro Glu 
            260                 265                 270         


Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met Arg Pro Pro 
        275                 280                 285             


Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu Ala Pro Glu 
    290                 295                 300                 


Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr Arg Met Phe 
305                 310                 315                 320 


Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala Ile Arg His 
                325                 330                 335     


Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser Glu Lys Gly 
            340                 345                 350         


Ala Val Trp Thr Val Asp Glu Leu Glu Phe 
        355                 360         


<210>  11
<211>  509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR extracellular domain

<400>  11

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys Asp Val Leu Met Thr Gln Thr Pro Leu Ser 
            20                  25                  30          


Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser 
        35                  40                  45              


Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu 
    50                  55                  60                  


Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn 
65                  70                  75                  80  


Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val 
            100                 105                 110         


Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Arg Thr Ser Gly Gly Gly 
        115                 120                 125             


Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile 
    130                 135                 140                 


Phe Pro Pro Ser Ser Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val 
145                 150                 155                 160 


Cys Phe Leu Asn Asn Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys 
                165                 170                 175     


Ile Asp Gly Ser Glu Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp 
            180                 185                 190         


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu 
        195                 200                 205             


Thr Lys Asp Glu Tyr Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr 
    210                 215                 220                 


His Lys Thr Ser Thr Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu 
225                 230                 235                 240 


Cys Gly Gly Gly Gly Gln Gly Gly Gly Gly Gln Gly Gly Gly Gly Gln 
                245                 250                 255     


Gly Gly Gly Gly Gln Gly Gly Gly Gly Gln Gly Gly Gly Gly Gln Gly 
            260                 265                 270         


Gly Gly Gly Gln Gly Gly Gly Gly Gln Gly Gly Gly Gly Gln Gly Gly 
        275                 280                 285             


Gly Gly Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro 
    290                 295                 300                 


Gly Ala Ser Val Arg Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
305                 310                 315                 320 


Ser Tyr His Ile His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 
                325                 330                 335     


Trp Ile Gly Trp Ile Tyr Pro Gly Asn Val Asn Thr Glu Tyr Asn Glu 
            340                 345                 350         


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
        355                 360                 365             


Ala Tyr Met His Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
    370                 375                 380                 


Phe Cys Ala Arg Glu Glu Ile Thr Tyr Ala Met Asp Tyr Trp Gly Gln 
385                 390                 395                 400 


Gly Thr Ser Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val 
                405                 410                 415     


Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met Val Thr 
            420                 425                 430         


Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val Thr 
        435                 440                 445             


Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro Ala Val 
    450                 455                 460                 


Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val Pro Ser 
465                 470                 475                 480 


Ser Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val Ala His Pro Ala 
                485                 490                 495     


Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp 
            500                 505                 


<210>  12
<211>  1530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR extracellular domain

<400>  12
atggacatga gggtccccgc tcagctcctg gggctcctgc tactctggct ccgaggtgcc       60

agatgtgatg ttttgatgac ccaaactcca ctctccctgc ctgtcagtct tggagatcaa      120

gcctccatct cttgcagatc tagtcagagc attgtacata gtaatggaaa cacctattta      180

gaatggtacc tgcagaaacc aggccagtct ccaaagctcc tgatctacaa agtttccaac      240

cgattttctg gggtcccaga caggttcagt ggcagtggat cagggacaga tttcacactc      300

aagatcagta gagtggaggc tgaggatctg ggagtttatt actgctttca aggttcacat      360

gttcctcgga cgtccggtgg aggcaccaag ctggaaatca aacgggctga tgctgcacca      420

actgtatcca tcttcccacc atccagtgag cagttaacat ctggaggtgc ctcagtcgtg      480

tgcttcttga acaacttcta ccccaaagac atcaatgtca agtggaagat tgatggcagt      540

gaacgacaaa atggcgtcct gaacagttgg actgatcagg acagcaaaga cagcacctac      600

agcatgagca gcaccctcac gttgaccaag gacgagtatg aacgacataa cagctatacc      660

tgtgaggcca ctcacaagac atcaacttca cccattgtca agagcttcaa caggaatgag      720

tgtggcggag gtgggcaggg aggcggcggg cagggtggcg gagggcaagg gggaggtggc      780

cagggcggcg ggggacaggg agggggcggt cagggcggag gcgggcaagg aggcggtggg      840

caagggggtg gcggacaggg cgggggaggc caggtccagc tgcagcagtc tggacctgag      900

ctggtgaagc ctggggcttc agtgaggata tcctgcaagg cttctggcta caccttcaca      960

agttaccata tacattgggt gaagcagagg cctggacagg gacttgagtg gattggatgg     1020

atttatcctg gaaatgttaa tactgagtac aatgagaagt tcaagggcaa ggccacactg     1080

actgcagaca aatcgtccag cacagcctac atgcacctca gcagcctgac ctctgaggac     1140

tctgcggtct atttctgtgc cagagaggag attacctatg ctatggacta ctggggtcaa     1200

ggaacctcag tcaccgtgtc ctcagccaaa acgacacccc catctgtcta tccactggcc     1260

cctggatctg ctgcccaaac taactccatg gtgaccctgg gatgcctggt caagggctat     1320

ttccctgagc cagtgacagt gacctggaac tctggatccc tgtccagcgg tgtgcacacc     1380

ttcccagctg tcctgcagtc tgacctctac actctgagca gctcagtgac tgtcccctcc     1440

agcacctggc ccagcgagac agtcacctgc aacgttgccc acccggccag cagcaccaag     1500

gtggacaaga aaattgtgcc cagggattgt                                      1530


<210>  13
<211>  45
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CD8 hinge

<400>  13

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  14
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  14
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  15
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 hinge

<400>  15

Cys Thr Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys 
1               5                   10                  15      


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
            20                  25                  30          


Pro Leu Phe Pro Gly Pro Ser Lys Pro 
        35                  40      


<210>  16
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 hinge

<400>  16
tgtaccattg aagttatgta tcctcctcct tacctagaca atgagaagag caatggaacc       60

attatccatg tgaaagggaa acacctttgt ccaagtcccc tatttcccgg accttctaag      120

ccc                                                                    123


<210>  17
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 transmembrane domain

<400>  17

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  18
<211>  81
<212>  DNA
<213>  CD28 transmembrane domain

<400>  18
ttctgggtgc tggtcgttgt gggcggcgtg ctggcctgct acagcctgct ggtgacagtg       60

gccttcatca tcttttgggt g                                                 81


<210>  19
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2R beta transmembrane domain

<400>  19

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  20
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2R beta transmembrane domain

<400>  20
attccgtggc tcggccacct cctcgtgggc ctcagcgggg cttttggctt catcatctta       60

gtgtacttgc tgatc                                                        75


<210>  21
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta activation domain

<400>  21

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  22
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 zeta activation domain

<400>  22
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gcgtagaggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggactca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgc                                336


<210>  23
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta activation domain

<400>  23

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu His Met Gln Ala Leu Pro Pro Arg 
        35                  40              


<210>  24
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD3 zeta activation domain

<400>  24
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttgcacat gcaggccctg      120

ccccctcgc                                                              129


<210>  25
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 co-stimulatory domain

<400>  25

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  26
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 co-stimulatory domain

<400>  26
aggagcaagc ggagcagact gctgcacagc gactacatga acatgacccc ccggaggcct       60

ggccccaccc ggaagcacta ccagccctac gcccctccca gggatttcgc cgcctaccgg      120

agc                                                                    123


<210>  27
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2R beta intracellular domain

<400>  27

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
65                  70                  75                  80  


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
                85                  90                  


<210>  28
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2R beta intracellular domain

<400>  28
aactgcagga acaccgggcc atggctgaag aaggtcctga agtgtaacac cccagacccc       60

tcgaagttct tttcccagct gagctcagag catggaggcg acgtccagaa gtggctctct      120

tcgcccttcc cctcatcgtc cttcagccct ggcggcctgg cacctgagat ctcgccacta      180

gaagtgctgg agagggacaa ggtgacgcag ctgctccccc tgaacactga tgcctacttg      240

tctctccaag aactccaggg tcaggaccca actcacttgg tg                         282


<210>  29
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T2A peptide

<400>  29
gagggcagag gcagcctgct gacatgtggc gacgtggaag agaaccctgg cccc             54


<210>  30
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A peptide

<400>  30

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  31
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A peptide

<400>  31

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  32
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A peptide

<400>  32

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  33
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A peptide

<400>  33

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  34
<211>  1293
<212>  DNA
<213>  Homo sapiens

<400>  34
atgcccaacc ccaggcctgg caagccctcg gccccttcct tggcccttgg cccatcccca       60

ggagcctcgc ccagctggag ggctgcaccc aaagcctcag acctgctggg ggcccggggc      120

ccagggggaa ccttccaggg ccgagatctt cgaggcgggg cccatgcctc ctcttcttcc      180

ttgaacccca tgccaccatc gcagctgcag ctgcccacac tgcccctagt catggtggca      240

ccctccgggg cacggctggg ccccttgccc cacttacagg cactcctcca ggacaggcca      300

catttcatgc accagctctc aacggtggat gcccacgccc ggacccctgt gctgcaggtg      360

caccccctgg agagcccagc catgatcagc ctcacaccac ccaccaccgc cactggggtc      420

ttctccctca aggcccggcc tggcctccca cctgggatca acgtggccag cctggaatgg      480

gtgtccaggg agccggcact gctctgcacc ttcccaaatc ccagtgcacc caggaaggac      540

agcacccttt cggctgtgcc ccagagctcc tacccactgc tggcaaatgg tgtctgcaag      600

tggcccggat gtgagaaggt cttcgaagag ccagaggact tcctcaagca ctgccaggcg      660

gaccatcttc tggatgagaa gggcagggca caatgtctcc tccagagaga gatggtacag      720

tctctggagc agcagctggt gctggagaag gagaagctga gtgccatgca ggcccacctg      780

gctgggaaaa tggcactgac caaggcttca tctgtggcat catccgacaa gggctcctgc      840

tgcatcgtag ctgctggcag ccaaggccct gtcgtcccag cctggtctgg cccccgggag      900

gcccctgaca gcctgtttgc tgtccggagg cacctgtggg gtagccatgg aaacagcaca      960

ttcccagagt tcctccacaa catggactac ttcaagttcc acaacatgcg accccctttc     1020

acctacgcca cgctcatccg ctgggccatc ctggaggctc cagagaagca gcggacactc     1080

aatgagatct accactggtt cacacgcatg tttgccttct tcagaaacca tcctgccacc     1140

tggaagaacg ccatccgcca caacctgagt ctgcacaagt gctttgtgcg ggtggagagc     1200

gagaaggggg ctgtgtggac cgtggatgag ctggagttcc gcaagaaacg gagccagagg     1260

cccagcaggt gttccaaccc tacacctggc ccc                                  1293


<210>  35
<211>  1086
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  minimal Foxp3

<400>  35
ggcggggccc atgcctcctc ttcttccttg aaccccatgc caccatcgca gctgcagctg       60

cccacactgc ccctagtcat ggtggcaccc tccggggcac ggctgggccc cttgccccac      120

ttacaggcac tcctccagga caggccacat ttcatgcacc agctctcaac ggtggatgcc      180

cacgcccgga cccctgtgct gcaggtgcac cccctggaga gcccagccat gatcagcctc      240

acaccaccca ccaccgccac tggggtcttc tccctcaagg cccggcctgg cctcccacct      300

gggatcaacg tggccagcct ggaatgggtg tccagggagc cggcactgct ctgcaccttc      360

ccaaatccca gtgcacccag gaaggacagc accctttcgg ctgtgcccca gagctcctac      420

ccactgctgg caaatggtgt ctgcaagtgg cccggatgtg agaaggtctt cgaagagcca      480

gaggacttcc tcaagcactg ccaggcggac catcttctgg atgagaaggg cagggcacaa      540

tgtctcctcc agagagagat ggtacagtct ctggagcagc agctggtgct ggagaaggag      600

aagctgagtg ccatgcaggc ccacctggct gggaaaatgg cactgaccaa ggcttcatct      660

gtggcatcat ccgacaaggg ctcctgctgc atcgtagctg ctggcagcca aggccctgtc      720

gtcccagcct ggtctggccc ccgggaggcc cctgacagcc tgtttgctgt ccggaggcac      780

ctgtggggta gccatggaaa cagcacattc ccagagttcc tccacaacat ggactacttc      840

aagttccaca acatgcgacc ccctttcacc tacgccacgc tcatccgctg ggccatcctg      900

gaggctccag agaagcagcg gacactcaat gagatctacc actggttcac acgcatgttt      960

gccttcttca gaaaccatcc tgccacctgg aagaacgcca tccgccacaa cctgagtctg     1020

cacaagtgct ttgtgcgggt ggagagcgag aagggggctg tgtggaccgt ggatgagctg     1080

gagttc                                                                1086


<210>  36
<211>  7625
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAR-Foxp3 vector

<400>  36
gcggccgctc gagtctagag tttaaaccgc gtctggaaca atcaacctct ggattacaaa       60

atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac      120

gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc      180

ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt      240

ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc      300

tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc      360

gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg      420

gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt      480

ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc      540

cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt      600

cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa      660

aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag      720

aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa      780

tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag      840

ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag      900

gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct      960

ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag     1020

gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag     1080

aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc     1140

atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg     1200

actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca     1260

tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg     1320

gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg     1380

cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc     1440

ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat     1500

ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt     1560

accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa     1620

ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg     1680

gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca     1740

gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg     1800

tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     1860

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     1920

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     1980

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     2040

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     2100

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     2160

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     2220

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     2280

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     2340

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     2400

gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc     2460

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     2520

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     2580

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     2640

acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa     2700

ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta     2760

ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt     2820

tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag     2880

tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca     2940

gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc     3000

tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt     3060

tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag     3120

ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt     3180

tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat     3240

ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt     3300

gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc     3360

ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat     3420

cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag     3480

ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt     3540

ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg     3600

gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta     3660

ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc     3720

gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta     3780

ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat     3840

ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct     3900

ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta     3960

ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat     4020

tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg     4080

ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc     4140

tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt     4200

ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga     4260

gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa     4320

gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat     4380

ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac     4440

tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata     4500

taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga     4560

gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct     4620

tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt     4680

ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg     4740

aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc     4800

gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga     4860

gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt     4920

cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg     4980

gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa     5040

atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat     5100

aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa     5160

gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc     5220

ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat     5280

ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa     5340

gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg     5400

gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat     5460

tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc     5520

atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg     5580

aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt     5640

gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga     5700

atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact     5760

ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag     5820

ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat     5880

tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta     5940

tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc     6000

cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca     6060

gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt     6120

ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa     6180

gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga     6240

gatccagttt atcgatgagt aattcataca aaaggactcg cccctgcctt ggggaatccc     6300

agggaccgtc gttaaactcc cactaacgta gaacccagag atcgctgcgt tcccgccccc     6360

tcacccgccc gctctcgtca tcactgaggt ggagaagagc atgcgtgagg ctccggtgcc     6420

cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc     6480

aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac     6540

tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg     6600

aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc     6660

cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacgccc     6720

ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc     6780

gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcttgggcg     6840

ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa     6900

gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag     6960

tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg     7020

cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc     7080

accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc     7140

gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg     7200

agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg     7260

ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc     7320

cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc     7380

gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc     7440

ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt     7500

ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca     7560

aagttttttt cttccatttc aggtgtcgtg attcgtttaa acttaagctt gaattctagg     7620

ccacc                                                                 7625


<210>  37
<211>  11467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti 1 JJ-1 (HB117) scFab huCD8aH_CD28Tm+ICD D_IL2RB CD3z (123)
       T2A FoxP3 wt vector

<400>  37
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccacgac gccagcgccg     5100

cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg     5160

tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatttc     5220

tgggtgctgg tcgttgtggg cggcgtgctg gcctgctaca gcctgctggt gacagtggcc     5280

ttcatcatct tttgggtgag gagcaagcgg agcagactgc tgcacagcga ctacatgaac     5340

atgacccccc ggaggcctgg ccccacccgg aagcactacc agccctacgc ccctcccagg     5400

gatttcgccg cctaccggag caactgcagg aacaccgggc catggctgaa gaaggtcctg     5460

aagtgtaaca ccccagaccc ctcgaagttc ttttcccagc tgagctcaga gcatggaggc     5520

gacgtccaga agtggctctc ttcgcccttc ccctcatcgt ccttcagccc tggcggcctg     5580

gcacctgaga tctcgccact agaagtgctg gagagggaca aggtgacgca gctgctcccc     5640

ctgaacactg atgcctactt gtctctccaa gaactccagg gtcaggaccc aactcacttg     5700

gtgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag     5760

ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgga caagcgtaga     5820

ggccgggacc ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac     5880

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag     5940

cgccggaggg gcaaggggca cgatggcctt taccagggac tcagtacagc caccaaggac     6000

acctacgacg cccttcacat gcaggccctg ccccctcgcc ccggggaggg cagaggcagc     6060

ctgctgacat gtggcgacgt ggaagagaac cctggcccca tgcccaaccc caggcctggc     6120

aagccctcgg ccccttcctt ggcccttggc ccatccccag gagcctcgcc cagctggagg     6180

gctgcaccca aagcctcaga cctgctgggg gcccggggcc cagggggaac cttccagggc     6240

cgagatcttc gaggcggggc ccatgcctcc tcttcttcct tgaaccccat gccaccatcg     6300

cagctgcagc tgcccacact gcccctagtc atggtggcac cctccggggc acggctgggc     6360

cccttgcccc acttacaggc actcctccag gacaggccac atttcatgca ccagctctca     6420

acggtggatg cccacgcccg gacccctgtg ctgcaggtgc accccctgga gagcccagcc     6480

atgatcagcc tcacaccacc caccaccgcc actggggtct tctccctcaa ggcccggcct     6540

ggcctcccac ctgggatcaa cgtggccagc ctggaatggg tgtccaggga gccggcactg     6600

ctctgcacct tcccaaatcc cagtgcaccc aggaaggaca gcaccctttc ggctgtgccc     6660

cagagctcct acccactgct ggcaaatggt gtctgcaagt ggcccggatg tgagaaggtc     6720

ttcgaagagc cagaggactt cctcaagcac tgccaggcgg accatcttct ggatgagaag     6780

ggcagggcac aatgtctcct ccagagagag atggtacagt ctctggagca gcagctggtg     6840

ctggagaagg agaagctgag tgccatgcag gcccacctgg ctgggaaaat ggcactgacc     6900

aaggcttcat ctgtggcatc atccgacaag ggctcctgct gcatcgtagc tgctggcagc     6960

caaggccctg tcgtcccagc ctggtctggc ccccgggagg cccctgacag cctgtttgct     7020

gtccggaggc acctgtgggg tagccatgga aacagcacat tcccagagtt cctccacaac     7080

atggactact tcaagttcca caacatgcga ccccctttca cctacgccac gctcatccgc     7140

tgggccatcc tggaggctcc agagaagcag cggacactca atgagatcta ccactggttc     7200

acacgcatgt ttgccttctt cagaaaccat cctgccacct ggaagaacgc catccgccac     7260

aacctgagtc tgcacaagtg ctttgtgcgg gtggagagcg agaagggggc tgtgtggacc     7320

gtggatgagc tggagttccg caagaaacgg agccagaggc ccagcaggtg ttccaaccct     7380

acacctggcc cctagcggcc gctcgagtct agagtttaaa ccgcgtctgg aacaatcaac     7440

ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta     7500

cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt     7560

tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg     7620

ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg     7680

gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca     7740

cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca     7800

ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg     7860

ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag     7920

cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc     7980

gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag     8040

tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga     8100

ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt     8160

acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa     8220

gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat     8280

ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag     8340

atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga     8400

agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga     8460

tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt     8520

ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg     8580

actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc     8640

cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc     8700

agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa     8760

gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga     8820

gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc     8880

ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac     8940

attgctagcg ttttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg     9000

tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata     9060

aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca     9120

ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc     9180

gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg     9240

cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     9300

tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     9360

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     9420

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     9480

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     9540

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     9600

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     9660

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     9720

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     9780

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta     9840

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     9900

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     9960

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag    10020

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc    10080

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact    10140

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt    10200

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta    10260

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta    10320

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc    10380

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat    10440

agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt    10500

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg    10560

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca    10620

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta    10680

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg    10740

cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact    10800

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg    10860

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt    10920

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga    10980

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc    11040

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa    11100

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga    11160

gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt    11220

cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt    11280

atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc    11340

caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga    11400

ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt    11460

agtagtt                                                              11467


<210>  38
<211>  11451
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD28H+Tm+ICD D_IL2RB CD3z (123) T2A FoxP3 
wt vector

<400>  38
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggatcgcccc tgccttgggg     2220

aatcccaggg accgtcgtta aactcccact aacgtagaac ccagagatcg ctgcgttccc     2280

gccccctcac ccgcccgctc tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc     2340

ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg     2400

gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc     2460

gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc     2520

gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt     2580

ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc     2640

acgcccctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga     2700

gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggct     2760

tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt     2820

cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca     2880

agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc     2940

gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc     3000

gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg     3060

cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt     3120

tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac     3180

gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc     3240

ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta     3300

gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga     3360

gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt     3420

ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt     3480

ggttcaaagt ttttttcttc catttcaggt gtcgtgattc gtttaaactt aagcttgaat     3540

tctaggccac catggacatg agggtccccg ctcagctcct ggggctcctg ctactctggc     3600

tccgaggtgc cagatgtgat gttttgatga cccaaactcc actctccctg cctgtcagtc     3660

ttggagatca agcctccatc tcttgcagat ctagtcagag cattgtacat agtaatggaa     3720

acacctattt agaatggtac ctgcagaaac caggccagtc tccaaagctc ctgatctaca     3780

aagtttccaa ccgattttct ggggtcccag acaggttcag tggcagtgga tcagggacag     3840

atttcacact caagatcagt agagtggagg ctgaggatct gggagtttat tactgctttc     3900

aaggttcaca tgttcctcgg acgtccggtg gaggcaccaa gctggaaatc aaacgggctg     3960

atgctgcacc aactgtatcc atcttcccac catccagtga gcagttaaca tctggaggtg     4020

cctcagtcgt gtgcttcttg aacaacttct accccaaaga catcaatgtc aagtggaaga     4080

ttgatggcag tgaacgacaa aatggcgtcc tgaacagttg gactgatcag gacagcaaag     4140

acagcaccta cagcatgagc agcaccctca cgttgaccaa ggacgagtat gaacgacata     4200

acagctatac ctgtgaggcc actcacaaga catcaacttc acccattgtc aagagcttca     4260

acaggaatga gtgtggcgga ggtgggcagg gaggcggcgg gcagggtggc ggagggcaag     4320

ggggaggtgg ccagggcggc gggggacagg gagggggcgg tcagggcgga ggcgggcaag     4380

gaggcggtgg gcaagggggt ggcggacagg gcgggggagg ccaggtccag ctgcagcagt     4440

ctggacctga gctggtgaag cctggggctt cagtgaggat atcctgcaag gcttctggct     4500

acaccttcac aagttaccat atacattggg tgaagcagag gcctggacag ggacttgagt     4560

ggattggatg gatttatcct ggaaatgtta atactgagta caatgagaag ttcaagggca     4620

aggccacact gactgcagac aaatcgtcca gcacagccta catgcacctc agcagcctga     4680

cctctgagga ctctgcggtc tatttctgtg ccagagagga gattacctat gctatggact     4740

actggggtca aggaacctca gtcaccgtgt cctcagccaa aacgacaccc ccatctgtct     4800

atccactggc ccctggatct gctgcccaaa ctaactccat ggtgaccctg ggatgcctgg     4860

tcaagggcta tttccctgag ccagtgacag tgacctggaa ctctggatcc ctgtccagcg     4920

gtgtgcacac cttcccagct gtcctgcagt ctgacctcta cactctgagc agctcagtga     4980

ctgtcccctc cagcacctgg cccagcgaga cagtcacctg caacgttgcc cacccggcca     5040

gcagcaccaa ggtggacaag aaaattgtgc ccagggattg taccattgaa gttatgtatc     5100

ctcctcctta cctagacaat gagaagagca atggaaccat tatccatgtg aaagggaaac     5160

acctttgtcc aagtccccta tttcccggac cttctaagcc cttctgggtg ctggtcgttg     5220

tgggcggcgt gctggcctgc tacagcctgc tggtgacagt ggccttcatc atcttttggg     5280

tgaggagcaa gcggagcaga ctgctgcaca gcgactacat gaacatgacc ccccggaggc     5340

ctggccccac ccggaagcac taccagccct acgcccctcc cagggatttc gccgcctacc     5400

ggagcaactg caggaacacc gggccatggc tgaagaaggt cctgaagtgt aacaccccag     5460

acccctcgaa gttcttttcc cagctgagct cagagcatgg aggcgacgtc cagaagtggc     5520

tctcttcgcc cttcccctca tcgtccttca gccctggcgg cctggcacct gagatctcgc     5580

cactagaagt gctggagagg gacaaggtga cgcagctgct ccccctgaac actgatgcct     5640

acttgtctct ccaagaactc cagggtcagg acccaactca cttggtgaga gtgaagttca     5700

gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat aacgagctca     5760

atctaggacg aagagaggag tacgatgttt tggacaagcg tagaggccgg gaccctgaga     5820

tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa ctgcagaaag     5880

ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg aggggcaagg     5940

ggcacgatgg cctttaccag ggactcagta cagccaccaa ggacacctac gacgcccttc     6000

acatgcaggc cctgccccct cgccccgggg agggcagagg cagcctgctg acatgtggcg     6060

acgtggaaga gaaccctggc cccatgccca accccaggcc tggcaagccc tcggcccctt     6120

ccttggccct tggcccatcc ccaggagcct cgcccagctg gagggctgca cccaaagcct     6180

cagacctgct gggggcccgg ggcccagggg gaaccttcca gggccgagat cttcgaggcg     6240

gggcccatgc ctcctcttct tccttgaacc ccatgccacc atcgcagctg cagctgccca     6300

cactgcccct agtcatggtg gcaccctccg gggcacggct gggccccttg ccccacttac     6360

aggcactcct ccaggacagg ccacatttca tgcaccagct ctcaacggtg gatgcccacg     6420

cccggacccc tgtgctgcag gtgcaccccc tggagagccc agccatgatc agcctcacac     6480

cacccaccac cgccactggg gtcttctccc tcaaggcccg gcctggcctc ccacctggga     6540

tcaacgtggc cagcctggaa tgggtgtcca gggagccggc actgctctgc accttcccaa     6600

atcccagtgc acccaggaag gacagcaccc tttcggctgt gccccagagc tcctacccac     6660

tgctggcaaa tggtgtctgc aagtggcccg gatgtgagaa ggtcttcgaa gagccagagg     6720

acttcctcaa gcactgccag gcggaccatc ttctggatga gaagggcagg gcacaatgtc     6780

tcctccagag agagatggta cagtctctgg agcagcagct ggtgctggag aaggagaagc     6840

tgagtgccat gcaggcccac ctggctggga aaatggcact gaccaaggct tcatctgtgg     6900

catcatccga caagggctcc tgctgcatcg tagctgctgg cagccaaggc cctgtcgtcc     6960

cagcctggtc tggcccccgg gaggcccctg acagcctgtt tgctgtccgg aggcacctgt     7020

ggggtagcca tggaaacagc acattcccag agttcctcca caacatggac tacttcaagt     7080

tccacaacat gcgaccccct ttcacctacg ccacgctcat ccgctgggcc atcctggagg     7140

ctccagagaa gcagcggaca ctcaatgaga tctaccactg gttcacacgc atgtttgcct     7200

tcttcagaaa ccatcctgcc acctggaaga acgccatccg ccacaacctg agtctgcaca     7260

agtgctttgt gcgggtggag agcgagaagg gggctgtgtg gaccgtggat gagctggagt     7320

tccgcaagaa acggagccag aggcccagca ggtgttccaa ccctacacct ggcccctagc     7380

ggccgctcga gtctagagtt taaaccgcgt ctggaacaat caacctctgg attacaaaat     7440

ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc     7500

tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt     7560

gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg     7620

cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg     7680

tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc     7740

cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt     7800

gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct     7860

gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg     7920

cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg     7980

gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa     8040

catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa     8100

gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg     8160

acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg     8220

ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc     8280

tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt     8340

ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga     8400

gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa     8460

gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat     8520

ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac     8580

tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata     8640

taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga     8700

gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct     8760

tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt     8820

ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt     8880

ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac     8940

cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt     9000

gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg     9060

gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt     9120

cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt     9180

tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc     9240

tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg     9300

ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg     9360

ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac     9420

gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg     9480

gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct     9540

ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg     9600

tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct     9660

gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac     9720

tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt     9780

tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc     9840

tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca     9900

ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat     9960

ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac    10020

gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt    10080

aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc    10140

aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg    10200

cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg    10260

ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc    10320

cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta    10380

ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg    10440

ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct    10500

ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta    10560

gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg    10620

ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga    10680

ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt    10740

gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca    10800

ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt    10860

cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt    10920

ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga    10980

aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt    11040

gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc    11100

gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt    11160

gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt    11220

ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg    11280

atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt    11340

accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt    11400

tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt t             11451


<210>  39
<211>  11260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD8aH_CD28Tm+ICD D_IL2RB CD3z (1) 
       T2A FoxP3 wt vector

<400>  39
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccacgac gccagcgccg     5100

cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg     5160

tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatttc     5220

tgggtgctgg tcgttgtggg cggcgtgctg gcctgctaca gcctgctggt gacagtggcc     5280

ttcatcatct tttgggtgag gagcaagcgg agcagactgc tgcacagcga ctacatgaac     5340

atgacccccc ggaggcctgg ccccacccgg aagcactacc agccctacgc ccctcccagg     5400

gatttcgccg cctaccggag caactgcagg aacaccgggc catggctgaa gaaggtcctg     5460

aagtgtaaca ccccagaccc ctcgaagttc ttttcccagc tgagctcaga gcatggaggc     5520

gacgtccaga agtggctctc ttcgcccttc ccctcatcgt ccttcagccc tggcggcctg     5580

gcacctgaga tctcgccact agaagtgctg gagagggaca aggtgacgca gctgctcccc     5640

ctgaacactg atgcctactt gtctctccaa gaactccagg gtcaggaccc aactcacttg     5700

gtgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag     5760

ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgca catgcaggcc     5820

ctgccccctc gccccgggga gggcagaggc agcctgctga catgtggcga cgtggaagag     5880

aaccctggcc ccatgcccaa ccccaggcct ggcaagccct cggccccttc cttggccctt     5940

ggcccatccc caggagcctc gcccagctgg agggctgcac ccaaagcctc agacctgctg     6000

ggggcccggg gcccaggggg aaccttccag ggccgagatc ttcgaggcgg ggcccatgcc     6060

tcctcttctt ccttgaaccc catgccacca tcgcagctgc agctgcccac actgccccta     6120

gtcatggtgg caccctccgg ggcacggctg ggccccttgc cccacttaca ggcactcctc     6180

caggacaggc cacatttcat gcaccagctc tcaacggtgg atgcccacgc ccggacccct     6240

gtgctgcagg tgcaccccct ggagagccca gccatgatca gcctcacacc acccaccacc     6300

gccactgggg tcttctccct caaggcccgg cctggcctcc cacctgggat caacgtggcc     6360

agcctggaat gggtgtccag ggagccggca ctgctctgca ccttcccaaa tcccagtgca     6420

cccaggaagg acagcaccct ttcggctgtg ccccagagct cctacccact gctggcaaat     6480

ggtgtctgca agtggcccgg atgtgagaag gtcttcgaag agccagagga cttcctcaag     6540

cactgccagg cggaccatct tctggatgag aagggcaggg cacaatgtct cctccagaga     6600

gagatggtac agtctctgga gcagcagctg gtgctggaga aggagaagct gagtgccatg     6660

caggcccacc tggctgggaa aatggcactg accaaggctt catctgtggc atcatccgac     6720

aagggctcct gctgcatcgt agctgctggc agccaaggcc ctgtcgtccc agcctggtct     6780

ggcccccggg aggcccctga cagcctgttt gctgtccgga ggcacctgtg gggtagccat     6840

ggaaacagca cattcccaga gttcctccac aacatggact acttcaagtt ccacaacatg     6900

cgaccccctt tcacctacgc cacgctcatc cgctgggcca tcctggaggc tccagagaag     6960

cagcggacac tcaatgagat ctaccactgg ttcacacgca tgtttgcctt cttcagaaac     7020

catcctgcca cctggaagaa cgccatccgc cacaacctga gtctgcacaa gtgctttgtg     7080

cgggtggaga gcgagaaggg ggctgtgtgg accgtggatg agctggagtt ccgcaagaaa     7140

cggagccaga ggcccagcag gtgttccaac cctacacctg gcccctagcg gccgctcgag     7200

tctagagttt aaaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat     7260

tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc     7320

ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct     7380

ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca     7440

ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt     7500

ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg     7560

cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga     7620

agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt     7680

ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc     7740

cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt     7800

gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat     7860

cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga     7920

ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc     7980

agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc     8040

ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga     8100

ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta     8160

caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag     8220

cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg     8280

gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt     8340

caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag     8400

gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc     8460

tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     8520

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt     8580

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt     8640

ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa     8700

agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct     8760

agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     8820

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     8880

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     8940

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     9000

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     9060

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     9120

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     9180

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     9240

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     9300

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     9360

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     9420

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     9480

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     9540

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     9600

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     9660

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     9720

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     9780

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     9840

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     9900

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     9960

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc    10020

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata    10080

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg    10140

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc    10200

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct    10260

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa    10320

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt    10380

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca    10440

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac    10500

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca    10560

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt    10620

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc    10680

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca    10740

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata    10800

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc    10860

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc    10920

cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa    10980

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    11040

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat    11100

aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa    11160

ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag    11220

aaattgtatt tgttaaatat gtactacaaa cttagtagtt                          11260


<210>  40
<211>  11245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD28H+Tm+ICD D_IL2RB CD3z (1) T2A 
       FoxP3 wt vector

<400>  40
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccattga agttatgtat     5100

cctcctcctt acctagacaa tgagaagagc aatggaacca ttatccatgt gaaagggaaa     5160

cacctttgtc caagtcccct atttcccgga ccttctaagc ccttctgggt gctggtcgtt     5220

gtgggcggcg tgctggcctg ctacagcctg ctggtgacag tggccttcat catcttttgg     5280

gtgaggagca agcggagcag actgctgcac agcgactaca tgaacatgac cccccggagg     5340

cctggcccca cccggaagca ctaccagccc tacgcccctc ccagggattt cgccgcctac     5400

cggagcaact gcaggaacac cgggccatgg ctgaagaagg tcctgaagtg taacacccca     5460

gacccctcga agttcttttc ccagctgagc tcagagcatg gaggcgacgt ccagaagtgg     5520

ctctcttcgc ccttcccctc atcgtccttc agccctggcg gcctggcacc tgagatctcg     5580

ccactagaag tgctggagag ggacaaggtg acgcagctgc tccccctgaa cactgatgcc     5640

tacttgtctc tccaagaact ccagggtcag gacccaactc acttggtgag agtgaagttc     5700

agcaggagcg cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc     5760

aatctaggac gaagagagga gtacgatgtt ttgcacatgc aggccctgcc ccctcgcccc     5820

ggggagggca gaggcagcct gctgacatgt ggcgacgtgg aagagaaccc tggccccatg     5880

cccaacccca ggcctggcaa gccctcggcc ccttccttgg cccttggccc atccccagga     5940

gcctcgccca gctggagggc tgcacccaaa gcctcagacc tgctgggggc ccggggccca     6000

gggggaacct tccagggccg agatcttcga ggcggggccc atgcctcctc ttcttccttg     6060

aaccccatgc caccatcgca gctgcagctg cccacactgc ccctagtcat ggtggcaccc     6120

tccggggcac ggctgggccc cttgccccac ttacaggcac tcctccagga caggccacat     6180

ttcatgcacc agctctcaac ggtggatgcc cacgcccgga cccctgtgct gcaggtgcac     6240

cccctggaga gcccagccat gatcagcctc acaccaccca ccaccgccac tggggtcttc     6300

tccctcaagg cccggcctgg cctcccacct gggatcaacg tggccagcct ggaatgggtg     6360

tccagggagc cggcactgct ctgcaccttc ccaaatccca gtgcacccag gaaggacagc     6420

accctttcgg ctgtgcccca gagctcctac ccactgctgg caaatggtgt ctgcaagtgg     6480

cccggatgtg agaaggtctt cgaagagcca gaggacttcc tcaagcactg ccaggcggac     6540

catcttctgg atgagaaggg cagggcacaa tgtctcctcc agagagagat ggtacagtct     6600

ctggagcagc agctggtgct ggagaaggag aagctgagtg ccatgcaggc ccacctggct     6660

gggaaaatgg cactgaccaa ggcttcatct gtggcatcat ccgacaaggg ctcctgctgc     6720

atcgtagctg ctggcagcca aggccctgtc gtcccagcct ggtctggccc ccgggaggcc     6780

cctgacagcc tgtttgctgt ccggaggcac ctgtggggta gccatggaaa cagcacattc     6840

ccagagttcc tccacaacat ggactacttc aagttccaca acatgcgacc ccctttcacc     6900

tacgccacgc tcatccgctg ggccatcctg gaggctccag agaagcagcg gacactcaat     6960

gagatctacc actggttcac acgcatgttt gccttcttca gaaaccatcc tgccacctgg     7020

aagaacgcca tccgccacaa cctgagtctg cacaagtgct ttgtgcgggt ggagagcgag     7080

aagggggctg tgtggaccgt ggatgagctg gagttccgca agaaacggag ccagaggccc     7140

agcaggtgtt ccaaccctac acctggcccc tagcggccgc tcgagtctag agtttaaacc     7200

gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta     7260

actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta     7320

ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt     7380

atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg     7440

caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt     7500

tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag     7560

gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc     7620

catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc     7680

cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc     7740

ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc     7800

ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac     7860

agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt     7920

tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag     7980

ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga     8040

tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac     8100

accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt     8160

tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt     8220

gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg     8280

cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata     8340

tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg     8400

gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg     8460

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac     8520

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt     8580

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca     8640

gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag     8700

agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg     8760

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     8820

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     8880

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     8940

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     9000

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     9060

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     9120

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     9180

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     9240

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     9300

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     9360

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     9420

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     9480

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     9540

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     9600

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     9660

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     9720

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     9780

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     9840

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     9900

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc     9960

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact    10020

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc    10080

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt    10140

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta    10200

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg    10260

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt    10320

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc    10380

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt    10440

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc    10500

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc    10560

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa    10620

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac    10680

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa    10740

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt    10800

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa    10860

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct    10920

gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag    10980

caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt    11040

gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca    11100

aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca    11160

tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta    11220

aatatgtact acaaacttag tagtt                                          11245


<210>  41
<211>  11260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD8aH CD28Tm D_IL2RB CD28ICD CD3z 
       (1) T2A FoxP3 wt vector

<400>  41
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccacgac gccagcgccg     5100

cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg     5160

tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatttc     5220

tgggtgctgg tcgttgtggg cggcgtgctg gcctgctaca gcctgctggt gacagtggcc     5280

ttcatcatct tttgggtgaa ctgcaggaac accgggccat ggctgaagaa ggtcctgaag     5340

tgtaacaccc cagacccctc gaagttcttt tcccagctga gctcagagca tggaggcgac     5400

gtccagaagt ggctctcttc gcccttcccc tcatcgtcct tcagccctgg cggcctggca     5460

cctgagatct cgccactaga agtgctggag agggacaagg tgacgcagct gctccccctg     5520

aacactgatg cctacttgtc tctccaagaa ctccagggtc aggacccaac tcacttggtg     5580

aggagcaagc ggagcagact gctgcacagc gactacatga acatgacccc ccggaggcct     5640

ggccccaccc ggaagcacta ccagccctac gcccctccca gggatttcgc cgcctaccgg     5700

agcagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag     5760

ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgca catgcaggcc     5820

ctgccccctc gccccgggga gggcagaggc agcctgctga catgtggcga cgtggaagag     5880

aaccctggcc ccatgcccaa ccccaggcct ggcaagccct cggccccttc cttggccctt     5940

ggcccatccc caggagcctc gcccagctgg agggctgcac ccaaagcctc agacctgctg     6000

ggggcccggg gcccaggggg aaccttccag ggccgagatc ttcgaggcgg ggcccatgcc     6060

tcctcttctt ccttgaaccc catgccacca tcgcagctgc agctgcccac actgccccta     6120

gtcatggtgg caccctccgg ggcacggctg ggccccttgc cccacttaca ggcactcctc     6180

caggacaggc cacatttcat gcaccagctc tcaacggtgg atgcccacgc ccggacccct     6240

gtgctgcagg tgcaccccct ggagagccca gccatgatca gcctcacacc acccaccacc     6300

gccactgggg tcttctccct caaggcccgg cctggcctcc cacctgggat caacgtggcc     6360

agcctggaat gggtgtccag ggagccggca ctgctctgca ccttcccaaa tcccagtgca     6420

cccaggaagg acagcaccct ttcggctgtg ccccagagct cctacccact gctggcaaat     6480

ggtgtctgca agtggcccgg atgtgagaag gtcttcgaag agccagagga cttcctcaag     6540

cactgccagg cggaccatct tctggatgag aagggcaggg cacaatgtct cctccagaga     6600

gagatggtac agtctctgga gcagcagctg gtgctggaga aggagaagct gagtgccatg     6660

caggcccacc tggctgggaa aatggcactg accaaggctt catctgtggc atcatccgac     6720

aagggctcct gctgcatcgt agctgctggc agccaaggcc ctgtcgtccc agcctggtct     6780

ggcccccggg aggcccctga cagcctgttt gctgtccgga ggcacctgtg gggtagccat     6840

ggaaacagca cattcccaga gttcctccac aacatggact acttcaagtt ccacaacatg     6900

cgaccccctt tcacctacgc cacgctcatc cgctgggcca tcctggaggc tccagagaag     6960

cagcggacac tcaatgagat ctaccactgg ttcacacgca tgtttgcctt cttcagaaac     7020

catcctgcca cctggaagaa cgccatccgc cacaacctga gtctgcacaa gtgctttgtg     7080

cgggtggaga gcgagaaggg ggctgtgtgg accgtggatg agctggagtt ccgcaagaaa     7140

cggagccaga ggcccagcag gtgttccaac cctacacctg gcccctagcg gccgctcgag     7200

tctagagttt aaaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat     7260

tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc     7320

ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct     7380

ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca     7440

ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt     7500

ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg     7560

cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga     7620

agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt     7680

ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc     7740

cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt     7800

gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat     7860

cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga     7920

ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc     7980

agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc     8040

ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga     8100

ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta     8160

caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag     8220

cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg     8280

gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt     8340

caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag     8400

gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc     8460

tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct     8520

aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt     8580

gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt     8640

ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa     8700

agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct     8760

agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     8820

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     8880

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     8940

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     9000

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     9060

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     9120

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     9180

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     9240

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     9300

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     9360

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     9420

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     9480

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     9540

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     9600

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     9660

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     9720

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     9780

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     9840

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     9900

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     9960

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc    10020

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata    10080

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg    10140

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc    10200

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct    10260

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa    10320

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt    10380

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca    10440

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac    10500

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca    10560

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt    10620

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc    10680

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca    10740

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata    10800

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc    10860

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc    10920

cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa    10980

tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca    11040

ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat    11100

aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa    11160

ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag    11220

aaattgtatt tgttaaatat gtactacaaa cttagtagtt                          11260


<210>  42
<211>  11254
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD8aH IL2RBTm D_IL2RB CD28ICD CD3z 
       (1) T2A FoxP3 wt vector

<400>  42
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccacgac gccagcgccg     5100

cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg     5160

tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatatt     5220

ccgtggctcg gccacctcct cgtgggcctc agcggggctt ttggcttcat catcttagtg     5280

tacttgctga tcaactgcag gaacaccggg ccatggctga agaaggtcct gaagtgtaac     5340

accccagacc cctcgaagtt cttttcccag ctgagctcag agcatggagg cgacgtccag     5400

aagtggctct cttcgccctt cccctcatcg tccttcagcc ctggcggcct ggcacctgag     5460

atctcgccac tagaagtgct ggagagggac aaggtgacgc agctgctccc cctgaacact     5520

gatgcctact tgtctctcca agaactccag ggtcaggacc caactcactt ggtgaggagc     5580

aagcggagca gactgctgca cagcgactac atgaacatga ccccccggag gcctggcccc     5640

acccggaagc actaccagcc ctacgcccct cccagggatt tcgccgccta ccggagcaga     5700

gtgaagttca gcaggagcgc agacgccccc gcgtaccagc agggccagaa ccagctctat     5760

aacgagctca atctaggacg aagagaggag tacgatgttt tgcacatgca ggccctgccc     5820

cctcgccccg gggagggcag aggcagcctg ctgacatgtg gcgacgtgga agagaaccct     5880

ggccccatgc ccaaccccag gcctggcaag ccctcggccc cttccttggc ccttggccca     5940

tccccaggag cctcgcccag ctggagggct gcacccaaag cctcagacct gctgggggcc     6000

cggggcccag ggggaacctt ccagggccga gatcttcgag gcggggccca tgcctcctct     6060

tcttccttga accccatgcc accatcgcag ctgcagctgc ccacactgcc cctagtcatg     6120

gtggcaccct ccggggcacg gctgggcccc ttgccccact tacaggcact cctccaggac     6180

aggccacatt tcatgcacca gctctcaacg gtggatgccc acgcccggac ccctgtgctg     6240

caggtgcacc ccctggagag cccagccatg atcagcctca caccacccac caccgccact     6300

ggggtcttct ccctcaaggc ccggcctggc ctcccacctg ggatcaacgt ggccagcctg     6360

gaatgggtgt ccagggagcc ggcactgctc tgcaccttcc caaatcccag tgcacccagg     6420

aaggacagca ccctttcggc tgtgccccag agctcctacc cactgctggc aaatggtgtc     6480

tgcaagtggc ccggatgtga gaaggtcttc gaagagccag aggacttcct caagcactgc     6540

caggcggacc atcttctgga tgagaagggc agggcacaat gtctcctcca gagagagatg     6600

gtacagtctc tggagcagca gctggtgctg gagaaggaga agctgagtgc catgcaggcc     6660

cacctggctg ggaaaatggc actgaccaag gcttcatctg tggcatcatc cgacaagggc     6720

tcctgctgca tcgtagctgc tggcagccaa ggccctgtcg tcccagcctg gtctggcccc     6780

cgggaggccc ctgacagcct gtttgctgtc cggaggcacc tgtggggtag ccatggaaac     6840

agcacattcc cagagttcct ccacaacatg gactacttca agttccacaa catgcgaccc     6900

cctttcacct acgccacgct catccgctgg gccatcctgg aggctccaga gaagcagcgg     6960

acactcaatg agatctacca ctggttcaca cgcatgtttg ccttcttcag aaaccatcct     7020

gccacctgga agaacgccat ccgccacaac ctgagtctgc acaagtgctt tgtgcgggtg     7080

gagagcgaga agggggctgt gtggaccgtg gatgagctgg agttccgcaa gaaacggagc     7140

cagaggccca gcaggtgttc caaccctaca cctggcccct agcggccgct cgagtctaga     7200

gtttaaaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg     7260

gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt     7320

atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc     7380

tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt     7440

ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga     7500

ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct     7560

gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga     7620

cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct     7680

gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc     7740

tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg     7800

cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag     7860

tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga     7920

ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt     7980

agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg     8040

aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca     8100

gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct     8160

agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt     8220

acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt     8280

tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa     8340

ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg     8400

cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg     8460

cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag     8520

ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc     8580

gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa     8640

tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat     8700

gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag     8760

agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt     8820

ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc     8880

taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc     8940

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct     9000

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     9060

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     9120

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     9180

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     9240

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     9300

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     9360

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     9420

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     9480

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     9540

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     9600

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc     9660

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     9720

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     9780

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     9840

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     9900

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     9960

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg    10020

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga    10080

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag    10140

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa    10200

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc    10260

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca    10320

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg    10380

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat    10440

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc    10500

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg    10560

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg    10620

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt    10680

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca    10740

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata    10800

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac    10860

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa    10920

gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta    10980

caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag    11040

ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca    11100

agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct    11160

gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg    11220

tatttgttaa atatgtacta caaacttagt agtt                                11254


<210>  43
<211>  11245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD28H CD28Tm D_IL2RB CD28ICD CD3z 
       (1) T2A FoxP3 wt vector

<400>  43
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccattga agttatgtat     5100

cctcctcctt acctagacaa tgagaagagc aatggaacca ttatccatgt gaaagggaaa     5160

cacctttgtc caagtcccct atttcccgga ccttctaagc ccttctgggt gctggtcgtt     5220

gtgggcggcg tgctggcctg ctacagcctg ctggtgacag tggccttcat catcttttgg     5280

gtgaactgca ggaacaccgg gccatggctg aagaaggtcc tgaagtgtaa caccccagac     5340

ccctcgaagt tcttttccca gctgagctca gagcatggag gcgacgtcca gaagtggctc     5400

tcttcgccct tcccctcatc gtccttcagc cctggcggcc tggcacctga gatctcgcca     5460

ctagaagtgc tggagaggga caaggtgacg cagctgctcc ccctgaacac tgatgcctac     5520

ttgtctctcc aagaactcca gggtcaggac ccaactcact tggtgaggag caagcggagc     5580

agactgctgc acagcgacta catgaacatg accccccgga ggcctggccc cacccggaag     5640

cactaccagc cctacgcccc tcccagggat ttcgccgcct accggagcag agtgaagttc     5700

agcaggagcg cagacgcccc cgcgtaccag cagggccaga accagctcta taacgagctc     5760

aatctaggac gaagagagga gtacgatgtt ttgcacatgc aggccctgcc ccctcgcccc     5820

ggggagggca gaggcagcct gctgacatgt ggcgacgtgg aagagaaccc tggccccatg     5880

cccaacccca ggcctggcaa gccctcggcc ccttccttgg cccttggccc atccccagga     5940

gcctcgccca gctggagggc tgcacccaaa gcctcagacc tgctgggggc ccggggccca     6000

gggggaacct tccagggccg agatcttcga ggcggggccc atgcctcctc ttcttccttg     6060

aaccccatgc caccatcgca gctgcagctg cccacactgc ccctagtcat ggtggcaccc     6120

tccggggcac ggctgggccc cttgccccac ttacaggcac tcctccagga caggccacat     6180

ttcatgcacc agctctcaac ggtggatgcc cacgcccgga cccctgtgct gcaggtgcac     6240

cccctggaga gcccagccat gatcagcctc acaccaccca ccaccgccac tggggtcttc     6300

tccctcaagg cccggcctgg cctcccacct gggatcaacg tggccagcct ggaatgggtg     6360

tccagggagc cggcactgct ctgcaccttc ccaaatccca gtgcacccag gaaggacagc     6420

accctttcgg ctgtgcccca gagctcctac ccactgctgg caaatggtgt ctgcaagtgg     6480

cccggatgtg agaaggtctt cgaagagcca gaggacttcc tcaagcactg ccaggcggac     6540

catcttctgg atgagaaggg cagggcacaa tgtctcctcc agagagagat ggtacagtct     6600

ctggagcagc agctggtgct ggagaaggag aagctgagtg ccatgcaggc ccacctggct     6660

gggaaaatgg cactgaccaa ggcttcatct gtggcatcat ccgacaaggg ctcctgctgc     6720

atcgtagctg ctggcagcca aggccctgtc gtcccagcct ggtctggccc ccgggaggcc     6780

cctgacagcc tgtttgctgt ccggaggcac ctgtggggta gccatggaaa cagcacattc     6840

ccagagttcc tccacaacat ggactacttc aagttccaca acatgcgacc ccctttcacc     6900

tacgccacgc tcatccgctg ggccatcctg gaggctccag agaagcagcg gacactcaat     6960

gagatctacc actggttcac acgcatgttt gccttcttca gaaaccatcc tgccacctgg     7020

aagaacgcca tccgccacaa cctgagtctg cacaagtgct ttgtgcgggt ggagagcgag     7080

aagggggctg tgtggaccgt ggatgagctg gagttccgca agaaacggag ccagaggccc     7140

agcaggtgtt ccaaccctac acctggcccc tagcggccgc tcgagtctag agtttaaacc     7200

gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta     7260

actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta     7320

ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt     7380

atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg     7440

caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt     7500

tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag     7560

gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc     7620

catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc     7680

cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc     7740

ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc     7800

ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac     7860

agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt     7920

tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag     7980

ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga     8040

tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac     8100

accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt     8160

tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt     8220

gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg     8280

cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata     8340

tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg     8400

gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg     8460

ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac     8520

tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt     8580

gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca     8640

gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag     8700

agagtgagag gccttgacat tgctagcgtt ttaccgtcga cctctagcta gagcttggcg     8760

taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac     8820

atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca     8880

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     8940

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc     9000

tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca     9060

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     9120

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     9180

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     9240

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     9300

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     9360

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     9420

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     9480

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     9540

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     9600

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     9660

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     9720

tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct     9780

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     9840

tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa     9900

agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc     9960

tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact    10020

acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc    10080

tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt    10140

ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta    10200

agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg    10260

tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt    10320

acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc    10380

agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt    10440

actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc    10500

tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc    10560

gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa    10620

ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac    10680

tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa    10740

aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt    10800

tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa    10860

tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct    10920

gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag    10980

caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt    11040

gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca    11100

aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca    11160

tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta    11220

aatatgtact acaaacttag tagtt                                          11245


<210>  44
<211>  11239
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pLenti scFab huCD28H IL2RBTm D_IL2RB CD28ICD CD3z 
       (1) T2A FoxP3 wt vector

<400>  44
ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac       60

acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact      120

gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa      180

taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga      240

gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga      300

gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc      360

tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc      420

ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag      480

cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt      540

gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca      600

gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc      660

gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc      720

aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa      780

ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga      840

aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc      900

aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg      960

tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc     1020

attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac     1080

caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca     1140

agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt     1200

gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca     1260

aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg     1320

ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc     1380

agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg     1440

caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg     1500

gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa     1560

ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag     1620

atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta     1680

atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg     1740

gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat     1800

ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta     1860

ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc     1920

ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac     1980

agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg     2040

ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca     2100

aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga     2160

cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg     2220

gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc     2280

cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc     2340

cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg     2400

ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt     2460

cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt     2520

cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg     2580

tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc     2640

cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg     2700

agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc     2760

ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt     2820

tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc     2880

aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg     2940

cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag     3000

cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg     3060

gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag     3120

ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga     3180

cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt     3240

cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt     3300

agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg     3360

agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat     3420

tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag     3480

tggttcaaag tttttttctt ccatttcagg tgtcgtgatt cgtttaaact taagcttgaa     3540

ttctaggcca ccatggacat gagggtcccc gctcagctcc tggggctcct gctactctgg     3600

ctccgaggtg ccagatgtga tgttttgatg acccaaactc cactctccct gcctgtcagt     3660

cttggagatc aagcctccat ctcttgcaga tctagtcaga gcattgtaca tagtaatgga     3720

aacacctatt tagaatggta cctgcagaaa ccaggccagt ctccaaagct cctgatctac     3780

aaagtttcca accgattttc tggggtccca gacaggttca gtggcagtgg atcagggaca     3840

gatttcacac tcaagatcag tagagtggag gctgaggatc tgggagttta ttactgcttt     3900

caaggttcac atgttcctcg gacgtccggt ggaggcacca agctggaaat caaacgggct     3960

gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac atctggaggt     4020

gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt caagtggaag     4080

attgatggca gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca ggacagcaaa     4140

gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta tgaacgacat     4200

aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt caagagcttc     4260

aacaggaatg agtgtggcgg aggtgggcag ggaggcggcg ggcagggtgg cggagggcaa     4320

gggggaggtg gccagggcgg cgggggacag ggagggggcg gtcagggcgg aggcgggcaa     4380

ggaggcggtg ggcaaggggg tggcggacag ggcgggggag gccaggtcca gctgcagcag     4440

tctggacctg agctggtgaa gcctggggct tcagtgagga tatcctgcaa ggcttctggc     4500

tacaccttca caagttacca tatacattgg gtgaagcaga ggcctggaca gggacttgag     4560

tggattggat ggatttatcc tggaaatgtt aatactgagt acaatgagaa gttcaagggc     4620

aaggccacac tgactgcaga caaatcgtcc agcacagcct acatgcacct cagcagcctg     4680

acctctgagg actctgcggt ctatttctgt gccagagagg agattaccta tgctatggac     4740

tactggggtc aaggaacctc agtcaccgtg tcctcagcca aaacgacacc cccatctgtc     4800

tatccactgg cccctggatc tgctgcccaa actaactcca tggtgaccct gggatgcctg     4860

gtcaagggct atttccctga gccagtgaca gtgacctgga actctggatc cctgtccagc     4920

ggtgtgcaca ccttcccagc tgtcctgcag tctgacctct acactctgag cagctcagtg     4980

actgtcccct ccagcacctg gcccagcgag acagtcacct gcaacgttgc ccacccggcc     5040

agcagcacca aggtggacaa gaaaattgtg cccagggatt gtaccattga agttatgtat     5100

cctcctcctt acctagacaa tgagaagagc aatggaacca ttatccatgt gaaagggaaa     5160

cacctttgtc caagtcccct atttcccgga ccttctaagc ccattccgtg gctcggccac     5220

ctcctcgtgg gcctcagcgg ggcttttggc ttcatcatct tagtgtactt gctgatcaac     5280

tgcaggaaca ccgggccatg gctgaagaag gtcctgaagt gtaacacccc agacccctcg     5340

aagttctttt cccagctgag ctcagagcat ggaggcgacg tccagaagtg gctctcttcg     5400

cccttcccct catcgtcctt cagccctggc ggcctggcac ctgagatctc gccactagaa     5460

gtgctggaga gggacaaggt gacgcagctg ctccccctga acactgatgc ctacttgtct     5520

ctccaagaac tccagggtca ggacccaact cacttggtga ggagcaagcg gagcagactg     5580

ctgcacagcg actacatgaa catgaccccc cggaggcctg gccccacccg gaagcactac     5640

cagccctacg cccctcccag ggatttcgcc gcctaccgga gcagagtgaa gttcagcagg     5700

agcgcagacg cccccgcgta ccagcagggc cagaaccagc tctataacga gctcaatcta     5760

ggacgaagag aggagtacga tgttttgcac atgcaggccc tgccccctcg ccccggggag     5820

ggcagaggca gcctgctgac atgtggcgac gtggaagaga accctggccc catgcccaac     5880

cccaggcctg gcaagccctc ggccccttcc ttggcccttg gcccatcccc aggagcctcg     5940

cccagctgga gggctgcacc caaagcctca gacctgctgg gggcccgggg cccaggggga     6000

accttccagg gccgagatct tcgaggcggg gcccatgcct cctcttcttc cttgaacccc     6060

atgccaccat cgcagctgca gctgcccaca ctgcccctag tcatggtggc accctccggg     6120

gcacggctgg gccccttgcc ccacttacag gcactcctcc aggacaggcc acatttcatg     6180

caccagctct caacggtgga tgcccacgcc cggacccctg tgctgcaggt gcaccccctg     6240

gagagcccag ccatgatcag cctcacacca cccaccaccg ccactggggt cttctccctc     6300

aaggcccggc ctggcctccc acctgggatc aacgtggcca gcctggaatg ggtgtccagg     6360

gagccggcac tgctctgcac cttcccaaat cccagtgcac ccaggaagga cagcaccctt     6420

tcggctgtgc cccagagctc ctacccactg ctggcaaatg gtgtctgcaa gtggcccgga     6480

tgtgagaagg tcttcgaaga gccagaggac ttcctcaagc actgccaggc ggaccatctt     6540

ctggatgaga agggcagggc acaatgtctc ctccagagag agatggtaca gtctctggag     6600

cagcagctgg tgctggagaa ggagaagctg agtgccatgc aggcccacct ggctgggaaa     6660

atggcactga ccaaggcttc atctgtggca tcatccgaca agggctcctg ctgcatcgta     6720

gctgctggca gccaaggccc tgtcgtccca gcctggtctg gcccccggga ggcccctgac     6780

agcctgtttg ctgtccggag gcacctgtgg ggtagccatg gaaacagcac attcccagag     6840

ttcctccaca acatggacta cttcaagttc cacaacatgc gacccccttt cacctacgcc     6900

acgctcatcc gctgggccat cctggaggct ccagagaagc agcggacact caatgagatc     6960

taccactggt tcacacgcat gtttgccttc ttcagaaacc atcctgccac ctggaagaac     7020

gccatccgcc acaacctgag tctgcacaag tgctttgtgc gggtggagag cgagaagggg     7080

gctgtgtgga ccgtggatga gctggagttc cgcaagaaac ggagccagag gcccagcagg     7140

tgttccaacc ctacacctgg cccctagcgg ccgctcgagt ctagagttta aaccgcgtct     7200

ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg     7260

ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt     7320

cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg     7380

agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc     7440

ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc     7500

tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc     7560

ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc     7620

tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg     7680

ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc     7740

gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa     7800

ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc     7860

taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt     7920

cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt     7980

tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct     8040

tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg     8100

gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc     8160

agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct     8220

gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc     8280

atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc     8340

ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg     8400

ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc     8460

tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta     8520

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact     8580

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta     8640

gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg     8700

agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca     8760

tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga     8820

gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt     8880

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     8940

atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc     9000

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     9060

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     9120

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     9180

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     9240

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     9300

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     9360

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     9420

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     9480

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     9540

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     9600

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     9660

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     9720

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     9780

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     9840

aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     9900

tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     9960

atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata    10020

cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg    10080

gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct    10140

gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt    10200

tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc    10260

tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga    10320

tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt    10380

aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc    10440

atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa    10500

tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca    10560

catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca    10620

aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct    10680

tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc    10740

gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa    10800

tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt    10860

tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc    10920

gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag    10980

catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa    11040

actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca    11100

tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact    11160

ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg    11220

tactacaaac ttagtagtt                                                 11239


<210>  45
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM


<220>
<221>  X
<222>  (2)..(3)
<223>  X can be any amino acid

<400>  45

Tyr Xaa Xaa Leu 
1               


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STAT5 recruitment Motif

<400>  46

Tyr Leu Ser Leu 
1               


