                         SEQUENCE LISTING

<110>  bluebird bio, Inc.
       Friedman, Kevin
       Perkins, Molly Reed
 
<120>  ANTI-BCMA CAR ANTIBODIES, CONJUGATES, AND METHODS OF USE

<130>  BLBD-099/01WO 315698-2946

<150>  US 62/685,043
<151>  2018-06-04

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR1

<400>  1

Lys Ala Ser Gln Asp Val Asp Thr Thr Val Ala 
1               5                   10      


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR2

<400>  2

Trp Ala Ser Thr Arg His Thr 
1               5           


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light Chain CDR3

<400>  3

Gln Gln Tyr Thr Phe Tyr Pro Tyr Thr 
1               5                   


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR1

<400>  4

Gly Tyr Thr Phe Thr Ser Tyr Trp Met His 
1               5                   10  


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR2

<400>  5

Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Thr 
    


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR6

<400>  6

Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Phe 
1               5                   10                  


<210>  7
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Varialble light chain

<400>  7

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Asp Thr Thr 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Thr Phe Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  8
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Variable heavy chain

<400>  8

Glu Val Gln Leu Gln Glu Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Thr Lys Ala Thr Leu Thr Val Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Arg Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Phe 
            100                 105                 110         


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120             


<210>  9
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR1

<400>  9

Lys Ala Ser Gln Asp Val Gly Thr Ala Val Ala 
1               5                   10      


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR2

<400>  10

Trp Ala Ser Thr Arg His Thr 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light Chain CDR3

<400>  11

Gln Gln Tyr Asn Thr Tyr Pro Tyr Thr 
1               5                   


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR1

<400>  12

Gly Tyr Thr Phe Thr Ser Tyr Trp Met His 
1               5                   10  


<210>  13
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR2

<400>  13

Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Thr 
    


<210>  14
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR6

<400>  14

Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Tyr 
1               5                   10                  


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Varialble light chain

<400>  15

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Thr Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  16
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Variable heavy chain

<400>  16

Glu Val Gln Leu Gln Glu Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Thr Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Arg Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120             


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR1

<400>  17

Lys Ala Ser Gln Asp Val Asp Thr Thr Val Ala 
1               5                   10      


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light chain CDR2

<400>  18

Trp Ala Ser Thr Arg His Thr 
1               5           


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Light Chain CDR3

<400>  19

Gln Gln Tyr Thr Phe Tyr Pro Tyr Thr 
1               5                   


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR1

<400>  20

Gly Tyr Thr Phe Thr Ser Tyr Trp Met His 
1               5                   10  


<210>  21
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR2

<400>  21

Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Thr 
    


<210>  22
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Heavy chain CDR6

<400>  22

Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Phe 
1               5                   10                  


<210>  23
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Varialble light chain

<400>  23

Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Asp Thr Thr 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Asn Val Gln Ser 
65                  70                  75                  80  


Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Thr Phe Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  24
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - Variable heavy chain

<400>  24

Glu Val Gln Leu Gln Glu Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Glu Ile Asn Pro Arg Asn Gly Arg Ser Asn Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Thr Lys Ala Thr Leu Thr Val Asp Glu Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Arg Glu Val His Tyr Tyr Gly Ser Asp Tyr Asp Ala Met Asp Phe 
            100                 105                 110         


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Pro 
        115                 120             


<210>  25
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - anti-BCMA CAR

<400>  25

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Val Leu Thr Gln Ser Pro Pro Ser Leu 
            20                  25                  30          


Ala Met Ser Leu Gly Lys Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 
        35                  40                  45              


Ser Val Thr Ile Leu Gly Ser His Leu Ile His Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Thr Leu Leu Ile Gln Leu Ala Ser Asn Val Gln 
65                  70                  75                  80  


Thr Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Asp Pro Val Glu Glu Asp Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Leu Gln Ser Arg Thr Ile Pro Arg Thr Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly 
    130                 135                 140                 


Glu Gly Ser Thr Lys Gly Gln Ile Gln Leu Val Gln Ser Gly Pro Glu 
145                 150                 155                 160 


Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly 
                165                 170                 175     


Tyr Thr Phe Thr Asp Tyr Ser Ile Asn Trp Val Lys Arg Ala Pro Gly 
            180                 185                 190         


Lys Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Glu Thr Arg Glu Pro 
        195                 200                 205             


Ala Tyr Ala Tyr Asp Phe Arg Gly Arg Phe Ala Phe Ser Leu Glu Thr 
    210                 215                 220                 


Ser Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Tyr Glu Asp 
225                 230                 235                 240 


Thr Ala Thr Tyr Phe Cys Ala Leu Asp Tyr Ser Tyr Ala Met Asp Tyr 
                245                 250                 255     


Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Ala Ala Ala Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  26

Asp Gly Gly Gly Ser 
1               5   


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  27

Thr Gly Glu Lys Pro 
1               5   


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  28

Gly Gly Arg Arg 
1               


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  29

Gly Gly Gly Gly Ser 
1               5   


<210>  30
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  30

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  31

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  32

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  33

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  34
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  34

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  35
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  35

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  36
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  36

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  37
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining light chain CDR-L3 motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  37

Phe Gly Xaa Gly 
1               


<210>  38
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H1motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(4)
<223>  Xaa is any amino acid

<400>  38

Cys Xaa Xaa Xaa 
1               


<210>  39
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H2motif

<400>  39

Leu Glu Trp Ile Gly 
1               5   


<210>  40
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H3 motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  40

Trp Gly Xaa Gly 
1               


