                         SEQUENCE LISTING

<110>  Baker Heart and Diabetes Institute
 
<120>  Therapeutic and Theranostic Agents

<130>  528437PCT

<141>  2019-06-21

<150>  AU2018902247
<151>  2018-06-22

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of scFv (SCE5) to activated platelets

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Met Phe Ser Arg Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Pro Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Ala Thr Tyr Thr Ser Arg Ser Asp Val Pro Asp Gln Thr 
            100                 105                 110         


Ser Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
        115                 120                 125             


Ser Ala Ser Ala Pro Lys Leu Glu Glu Gly Glu Phe Ser Glu Ala Arg 
    130                 135                 140                 


Val Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
145                 150                 155                 160 


Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Asn Phe Tyr Ala 
                165                 170                 175     


Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Thr Leu Val Ile Tyr 
            180                 185                 190         


Gly Leu Ser Lys Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Ala Ser 
        195                 200                 205             


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
    210                 215                 220                 


Asp Glu Ala Asp Tyr Tyr Cys Leu Leu Tyr Tyr Gly Gly Gly Gln Gln 
225                 230                 235                 240 


Gly Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg 
                245                 250             


<210>  2
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH (SCE5)

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Met Phe Ser Arg Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Pro Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Ala Thr Tyr Thr Ser Arg Ser Asp Val Pro Asp Gln Thr 
            100                 105                 110         


Ser Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
        115                 120                 125             


Ser Ala Ser 
    130     


<210>  3
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL (SCE5)

<400>  3

Val Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Asn Phe Tyr Ala 
            20                  25                  30          


Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Thr Leu Val Ile Tyr 
        35                  40                  45              


Gly Leu Ser Lys Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Ala Ser 
    50                  55                  60                  


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Leu Leu Tyr Tyr Gly Gly Gly Gln Gln 
                85                  90                  95      


Gly Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg 
            100                 105                 


<210>  4
<211>  899
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding scFv (SCE5)

<400>  4
ccatggagac agacacactc ctgctatggg tactgctgct ctgggttcca ggttccactg       60

gtgacgcggc ccagccggcc aggcgcgccg tacgaagctt ggtaccgagc tcggatccac      120

tccagtgtgg tggaattctg caggtgcagc tgcaggaaag cggcggaggc ctggtgcagc      180

ctggcggcag cctgagactg tcttgcgccg ccagcggctt catgttcagc agatacgcca      240

tgagctgggt ccgccaggcc cctggaaagg gacctgagtg ggtgtccggc atcagcggct      300

ctggcggcag cacctactac gccgacagcg tgaagggccg gttcaccgtg tctcgggaca      360

acagcaagaa caccctgtac ctgcagatga acagcctgcg ggccgaggac accgccgtgt      420

actactgcgc ccggggtgct acctacacct ccagaagcga cgtgcccgac cagaccagct      480

tcgattactg gggccagggc acactggtca cagtgtctag cggcagcgcc agcgccccca      540

agctggaaga gggcgagttt agcgaggccc gggtgtccga gctgacacag gatcctgccg      600

tgtccgtggc tctgggccag acagtgcgga tcacctgtca gggcgacagc ctgaggaact      660

tctacgccag ctggtatcag cagaagcccg gacaggcccc aaccctggtc atctacggcc      720

tgagcaagcg gcccagcggc atccccgaca gattcagcgc cagcagcagc ggcaacaccg      780

ccagcctgac cattactggc gcccaggccg aggacgaggc cgactactac tgcctgctgt      840

actatggcgg cggacagcag ggcgtgttcg gcggaggcac caagctgacc gtgctgaga       899


<210>  5
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of scFv (anti-LIBS) to activated platelets

<400>  5

Met Ala Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro 
1               5                   10                  15      


Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ile Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu 
        35                  40                  45              


Trp Val Ala Thr Ile Arg Ser Gly Gly Asp Asn Thr Tyr Tyr Pro Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Lys 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Leu Tyr 
                85                  90                  95      


Tyr Cys Ala Ile Tyr Tyr Gly Asn Tyr Gly Gly Leu Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro Lys Leu 
        115                 120                 125             


Glu Glu Gly Glu Phe Ser Glu Ala Arg Val Asp Ile Leu Met Thr Gln 
    130                 135                 140                 


Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ala Trp Tyr Gln Gln 
                165                 170                 175     


Lys Gln Gly Lys Ser Pro Gln Leu Leu Val Tyr Asn Ala Lys Thr Leu 
            180                 185                 190         


Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln 
        195                 200                 205             


Tyr Ser Leu Lys Ile Asn Ser Leu Gln Pro Glu Asp Phe Gly Ser Tyr 
    210                 215                 220                 


Tyr Cys Gln His Phe Trp Ser Thr Pro Tyr Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala Ala Ala Gly Ser 
                245                 250                 


<210>  6
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VH (anti-LIBS)

<400>  6

Met Ala Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Lys Pro 
1               5                   10                  15      


Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Ser Tyr Ile Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu 
        35                  40                  45              


Trp Val Ala Thr Ile Arg Ser Gly Gly Asp Asn Thr Tyr Tyr Pro Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Lys 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Leu Tyr 
                85                  90                  95      


Tyr Cys Ala Ile Tyr Tyr Gly Asn Tyr Gly Gly Leu Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ala 
        115                 120     


<210>  7
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of VL (anti-LIBS)

<400>  7

Val Asp Ile Leu Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn Ile His Asn 
            20                  25                  30          


Tyr Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu 
        35                  40                  45              


Val Tyr Asn Ala Lys Thr Leu Ala Asp Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp Ser Thr Pro 
                85                  90                  95      


Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp 
            100                 105                 110     


<210>  8
<211>  796
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding scFv (anti- LIBS)

<400>  8
atggcgcagg tgcagctgca gcagtctggg ggaggcttag tgaagcctgg agggtccctg       60

aaactctcct gcgcagcctc tggattcact ttcagtagct atatcatgtc ttgggttcgc      120

cagactccgg agaagaggct ggagtgggtc gcaaccatta gaagtggtgg tgataacacc      180

tactatccag acagtgtgaa gggtcgattc accatctcca gagacaatgc caagaacaag      240

ttgtacctgc aaatgagcag tctgaggtct gaggacacgg ccttgtatta ctgtgcaatc      300

tactatggta actacggggg gcttgcttac tggggccaag ggactctggt cactgtctct      360

gcagccaaaa cgacacccaa gcttgaagaa ggtgaatttt cagaagcacg cgtagatatc      420

ttgatgaccc aatctccagc ctccctatct gcatctgtgg gagaaactgt caccatcaca      480

tgtcgagcaa gtgggaatat tcacaattat ttagcatggt atcagcagaa acagggaaaa      540

tctcctcagc tcctggtcta taatgcaaaa accttagcag atggtgtgcc atcaaggttc      600

agtggcagtg gatcaggaac acaatattct ctcaagatca acagcctgca gcctgaagat      660

tttgggagtt attactgtca acatttttgg agtactccgt acacgttcgg aggggggacc      720

aagctggaaa taaaacgggc tgatgctgcg gccgctggat ccgaacaaaa gctgatctca      780

gaagaagacc taaact                                                      796


