                         SEQUENCE LISTING

<110>  Genomatica, Inc.
       Nagarajan, Harish
       Yang, Tae Hoon
       Khodayari, Ali
 
<120>  Engineered Microorganisms with G3P -> 3PG Enzyme and/or 
       Fructose-1,6-Bisphosphatase Including those Having Synthetic or 
       Enhanced Methylotrophy

<130>  GNO0088/WO

<150>  US 62/690,209
<151>  2018-06-26

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  481
<212>  PRT
<213>  Bacillus methanolicus

<400>  1

Met Gln Val Leu Glu Lys Asp Phe Lys Phe Leu Leu Asn Gly Glu Trp 
1               5                   10                  15      


Ser Phe Ser Ser Ser Asn Gln Phe Ile Asn Ile Tyr Ser Pro Ser Thr 
            20                  25                  30          


Gly Glu Val Val Gly Arg Val Pro Ala Met Thr Lys Asp Glu Ile Asp 
        35                  40                  45              


Lys Ala Val Ala Gly Ala Arg Gln Ala Gln Lys Lys Trp Glu Lys Leu 
    50                  55                  60                  


Ala Val Ser Glu Arg Ala Lys Ile Leu His Ala Trp Ala Asp Glu Leu 
65                  70                  75                  80  


Val Lys Met Ile Asp Val Ile Ala Pro Met Ile Met Asn Glu Val Gly 
                85                  90                  95      


Lys Asn Ile Ser Thr Ala Arg Lys Glu Val Thr Arg Thr Ala Glu Ile 
            100                 105                 110         


Ile Arg Tyr Thr Ala Glu Glu Gly Val Arg Ile His Gly Glu Phe Ile 
        115                 120                 125             


Asn Gly Gly Ser Leu Asp Ala Asp Ser Ser Ile Lys Thr Ala Ile Val 
    130                 135                 140                 


Glu Lys Lys Pro Leu Gly Val Ile Leu Ala Ile Ser Pro Phe Asn Tyr 
145                 150                 155                 160 


Pro Ile Asn Leu Ala Ala Thr Lys Ile Ala Pro Ala Leu Ile Ala Gly 
                165                 170                 175     


Asn Ala Val Val Phe Lys Pro Ala Thr Gln Gly Ala Ile Ser Gly Leu 
            180                 185                 190         


Leu Met Ile Gln Ala Leu Glu Asn Ala Gly Leu Pro Lys Gly Leu Val 
        195                 200                 205             


Asn Thr Val Thr Gly Lys Gly Ser Glu Ile Gly Asp Tyr Ile Ile Thr 
    210                 215                 220                 


His Pro Phe Ile Asp Met Ile Ser Phe Thr Gly Gly Thr Gly Thr Gly 
225                 230                 235                 240 


Gln Asn Ile Ala Arg Lys Ala Ser Met Ile Pro Leu Val Leu Glu Leu 
                245                 250                 255     


Gly Gly Lys Asp Pro Ala Ile Val Leu Glu Asp Ala Asp Leu Glu Leu 
            260                 265                 270         


Ala Ala Thr Glu Ile Val Ser Gly Ala Phe Ser Tyr Ser Gly Gln Arg 
        275                 280                 285             


Cys Thr Ala Ile Lys Arg Val Leu Val Leu Lys Asp Val Ala Ala Pro 
    290                 295                 300                 


Leu Ile Glu Lys Ile Lys Glu Lys Val Glu Lys Leu Thr Val Gly Arg 
305                 310                 315                 320 


Pro Glu Asp Asp Ala Asp Ile Thr Pro Leu Ile Asp Glu Ser Ser Ala 
                325                 330                 335     


Asp Phe Val Gln Gly Leu Ile Asp Asp Ala Leu Ala Lys Gly Ala Ser 
            340                 345                 350         


Leu Ile Thr Gly Asn Lys Arg Glu Lys Asn Leu Ile Tyr Pro Thr Val 
        355                 360                 365             


Leu Ser Asn Val Thr Lys Asp Met Lys Val Ala Trp Glu Glu Pro Phe 
    370                 375                 380                 


Gly Pro Val Leu Pro Cys Ile Ile Val Asp Ser Val Glu Glu Ala Ile 
385                 390                 395                 400 


Glu Ile Ala Asn Glu Ser Glu Phe Gly Leu Gln Ala Ser Val Phe Thr 
                405                 410                 415     


Ser Asn Ile Glu Lys Ala Phe Thr Ile Ala Ser Ser Leu Asp Val Gly 
            420                 425                 430         


Ser Val Gln Ile Asn Gly Arg Thr Glu Arg Gly Pro Asp His Phe Pro 
        435                 440                 445             


Phe Ser Ala Val Lys Asn Ser Gly Leu Gly Ser Gln Gly Ile Arg Gln 
    450                 455                 460                 


Ser Ile Ile Ser Met Met Arg Asp Lys Val Thr Val Ile Asn Leu Lys 
465                 470                 475                 480 


Lys 
    


<210>  2
<211>  211
<212>  PRT
<213>  Bacillus methanolicus

<400>  2

Met Glu Leu Gln Leu Ala Leu Asp Leu Val Asn Ile Glu Glu Ala Lys 
1               5                   10                  15      


Gln Val Val Ala Glu Val Gln Glu Tyr Val Asp Ile Val Glu Ile Gly 
            20                  25                  30          


Thr Pro Val Ile Lys Ile Trp Gly Leu Gln Ala Val Lys Ala Val Lys 
        35                  40                  45              


Asp Ala Phe Pro His Leu Gln Val Leu Ala Asp Met Lys Thr Met Asp 
    50                  55                  60                  


Ala Ala Ala Tyr Glu Val Ala Lys Ala Ala Glu His Gly Ala Asp Ile 
65                  70                  75                  80  


Val Thr Ile Leu Ala Ala Ala Glu Asp Val Ser Ile Lys Gly Ala Val 
                85                  90                  95      


Glu Glu Ala Lys Lys Leu Gly Lys Lys Ile Leu Val Asp Met Ile Ala 
            100                 105                 110         


Val Lys Asn Leu Glu Glu Arg Ala Lys Gln Val Asp Glu Met Gly Val 
        115                 120                 125             


Asp Tyr Ile Cys Val His Ala Gly Tyr Asp Leu Gln Ala Val Gly Lys 
    130                 135                 140                 


Asn Pro Leu Asp Asp Leu Lys Arg Ile Lys Ala Val Val Lys Asn Ala 
145                 150                 155                 160 


Lys Thr Ala Ile Ala Gly Gly Ile Lys Leu Glu Thr Leu Pro Glu Val 
                165                 170                 175     


Ile Lys Ala Glu Pro Asp Leu Val Ile Val Gly Gly Gly Ile Ala Asn 
            180                 185                 190         


Gln Thr Asp Lys Lys Ala Ala Ala Glu Lys Ile Asn Lys Leu Val Lys 
        195                 200                 205             


Gln Gly Leu 
    210     


<210>  3
<211>  184
<212>  PRT
<213>  Bacillus methanolicus

<400>  3

Met Leu Thr Thr Glu Phe Leu Ala Glu Ile Val Lys Glu Leu Asn Ser 
1               5                   10                  15      


Ser Val Asn Gln Ile Ala Asp Glu Glu Ala Glu Ala Leu Val Asn Gly 
            20                  25                  30          


Ile Leu Gln Ser Lys Lys Val Phe Val Ala Gly Ala Gly Arg Ser Gly 
        35                  40                  45              


Phe Met Ala Lys Ser Phe Ala Met Arg Met Met His Met Gly Ile Asp 
    50                  55                  60                  


Ala Tyr Val Val Gly Glu Thr Val Thr Pro Asn Tyr Glu Lys Glu Asp 
65                  70                  75                  80  


Ile Leu Ile Ile Gly Ser Gly Ser Gly Glu Thr Lys Ser Leu Val Ser 
                85                  90                  95      


Met Ala Gln Lys Ala Lys Ser Ile Gly Gly Thr Ile Ala Ala Val Thr 
            100                 105                 110         


Ile Asn Pro Glu Ser Thr Ile Gly Gln Leu Ala Asp Ile Val Ile Lys 
        115                 120                 125             


Met Pro Gly Ser Pro Lys Asp Lys Ser Glu Ala Arg Glu Thr Ile Gln 
    130                 135                 140                 


Pro Met Gly Ser Leu Phe Glu Gln Thr Leu Leu Leu Phe Tyr Asp Ala 
145                 150                 155                 160 


Val Ile Leu Arg Phe Met Glu Lys Lys Gly Leu Asp Thr Lys Thr Met 
                165                 170                 175     


Tyr Gly Arg His Ala Asn Leu Glu 
            180                 


<210>  4
<211>  382
<212>  PRT
<213>  Bacillus methanolicus

<400>  4

Met Thr Thr Asn Phe Phe Ile Pro Pro Ala Ser Val Ile Gly Arg Gly 
1               5                   10                  15      


Ala Val Lys Glu Val Gly Thr Arg Leu Lys Gln Ile Gly Ala Lys Lys 
            20                  25                  30          


Ala Leu Ile Val Thr Asp Ala Phe Leu His Ser Thr Gly Leu Ser Glu 
        35                  40                  45              


Glu Val Ala Lys Asn Ile Arg Glu Ala Gly Val Asp Val Ala Ile Phe 
    50                  55                  60                  


Pro Lys Ala Gln Pro Asp Pro Ala Asp Thr Gln Val His Glu Gly Val 
65                  70                  75                  80  


Asp Val Phe Lys Gln Glu Asn Cys Asp Ser Leu Val Ser Ile Gly Gly 
                85                  90                  95      


Gly Ser Ser His Asp Thr Ala Lys Ala Ile Gly Leu Val Ala Ala Asn 
            100                 105                 110         


Gly Gly Arg Ile Asn Asp Tyr Gln Gly Val Asn Ser Val Glu Lys Pro 
        115                 120                 125             


Val Val Pro Val Val Ala Ile Thr Thr Thr Ala Gly Thr Gly Ser Glu 
    130                 135                 140                 


Thr Thr Ser Leu Ala Val Ile Thr Asp Ser Ala Arg Lys Val Lys Met 
145                 150                 155                 160 


Pro Val Ile Asp Glu Lys Ile Thr Pro Thr Val Ala Ile Val Asp Pro 
                165                 170                 175     


Glu Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ile Ala Thr Gly Met 
            180                 185                 190         


Asp Ala Leu Ser His Ala Ile Glu Ala Tyr Val Ala Lys Gly Ala Thr 
        195                 200                 205             


Pro Val Thr Asp Ala Phe Ala Ile Gln Ala Met Lys Leu Ile Asn Glu 
    210                 215                 220                 


Tyr Leu Pro Lys Ala Val Ala Asn Gly Glu Asp Ile Glu Ala Arg Glu 
225                 230                 235                 240 


Lys Met Ala Tyr Ala Gln Tyr Met Ala Gly Val Ala Phe Asn Asn Gly 
                245                 250                 255     


Gly Leu Gly Leu Val His Ser Ile Ser His Gln Val Gly Gly Val Tyr 
            260                 265                 270         


Lys Leu Gln His Gly Ile Cys Asn Ser Val Asn Met Pro His Val Cys 
        275                 280                 285             


Ala Phe Asn Leu Ile Ala Lys Thr Glu Arg Phe Ala His Ile Ala Glu 
    290                 295                 300                 


Leu Leu Gly Glu Asn Val Ala Gly Leu Ser Thr Ala Ala Ala Ala Glu 
305                 310                 315                 320 


Arg Ala Ile Val Ala Leu Glu Arg Ile Asn Lys Ser Phe Gly Ile Pro 
                325                 330                 335     


Ser Gly Tyr Ala Glu Met Gly Val Lys Glu Glu Asp Ile Glu Leu Leu 
            340                 345                 350         


Ala Lys Asn Ala Tyr Glu Asp Val Cys Thr Gln Ser Asn Pro Arg Val 
        355                 360                 365             


Pro Thr Val Gln Asp Ile Ala Gln Ile Ile Lys Asn Ala Met 
    370                 375                 380         


<210>  5
<211>  319
<212>  PRT
<213>  Bacillus methanolicus

<400>  5

Met Lys Lys Ile Gly Val Leu Thr Ser Gly Gly Asp Ser Pro Gly Met 
1               5                   10                  15      


Asn Ala Ala Ile Arg Ala Val Val Arg Lys Ala Ile Tyr His Asn Leu 
            20                  25                  30          


Glu Val Tyr Gly Val Tyr Gly Gly Tyr Leu Gly Leu Ile Asn Gly Asn 
        35                  40                  45              


Ile Lys Lys Leu Glu Leu Gly Ser Val Gly Asp Ile Ile His Arg Gly 
    50                  55                  60                  


Gly Thr Met Leu Tyr Ser Ala Arg Cys Glu Glu Phe Lys Thr Lys Glu 
65                  70                  75                  80  


Gly Gln Leu Lys Gly Val Glu Gln Leu Lys Lys His Gly Ile Asp Gly 
                85                  90                  95      


Leu Val Val Ile Gly Gly Asp Gly Ser Tyr Arg Gly Ala Lys Ala Leu 
            100                 105                 110         


Thr Glu Leu Gly Phe Pro Cys Val Gly Val Pro Gly Thr Ile Asp Asn 
        115                 120                 125             


Asp Ile Pro Gly Thr Glu His Thr Ile Gly Phe Asp Thr Ala Leu Asn 
    130                 135                 140                 


Thr Val Ile Asp Ala Ile Asp Lys Ile Arg Asp Thr Ala Ser Ser His 
145                 150                 155                 160 


Glu Arg Thr Phe Val Val Glu Val Met Gly Arg Asn Ala Gly Asp Ile 
                165                 170                 175     


Ala Leu Trp Ala Gly Leu Ala Gly Gly Ala Glu Ala Ile Val Ile Pro 
            180                 185                 190         


Glu Glu Asn Tyr Asp Ile Asn Glu Ile Ala Asp Arg Leu Lys Arg Ser 
        195                 200                 205             


His Glu Arg Gly Lys Arg His Ser Ile Ile Ile Val Ala Glu Gly Val 
    210                 215                 220                 


Cys Arg Gly Glu Glu Leu Ser Lys Gln Ile Thr Glu Ala Thr Gly Phe 
225                 230                 235                 240 


Ala Thr Trp Val Thr Val Leu Gly His Val Gln Arg Gly Gly Ser Pro 
                245                 250                 255     


Ser Ala Phe Asp Arg Val Leu Ala Ser Arg Leu Gly Ala Arg Ala Val 
            260                 265                 270         


Glu Leu Leu Ile Glu Gly Lys Gly Gly Arg Ala Val Gly Ile Arg Lys 
        275                 280                 285             


Asn Gln Val Val Asp Tyr Asp Phe Asp Glu Val Phe Ser Asn Glu His 
    290                 295                 300                 


Thr Leu Asp Leu Glu Leu Cys Lys Leu Ser Lys Glu Leu Ser Ile 
305                 310                 315                 


<210>  6
<211>  214
<212>  PRT
<213>  Bacillus methanolicus

<400>  6

Met Ile Lys Ile Ala Pro Ser Ile Leu Ser Ala Asn Phe Ala Arg Leu 
1               5                   10                  15      


Glu Glu Glu Ile Lys Asp Val Glu Arg Gly Gly Ala Asp Tyr Ile His 
            20                  25                  30          


Val Asp Val Met Asp Gly His Phe Val Pro Asn Ile Thr Ile Gly Pro 
        35                  40                  45              


Leu Ile Val Glu Ala Ile Arg Pro Val Thr Asn Leu Pro Leu Asp Val 
    50                  55                  60                  


His Leu Met Ile Glu Asn Pro Asp Gln Tyr Ile Gly Thr Phe Ala Lys 
65                  70                  75                  80  


Ala Gly Ala Asp Ile Leu Ser Val His Val Glu Ala Cys Thr His Leu 
                85                  90                  95      


His Arg Thr Ile Gln Tyr Ile Lys Ser Glu Gly Ile Lys Ala Gly Val 
            100                 105                 110         


Val Leu Asn Pro His Thr Pro Val Ser Met Ile Glu His Val Ile Glu 
        115                 120                 125             


Asp Val Asp Leu Val Leu Leu Met Thr Val Asn Pro Gly Phe Gly Gly 
    130                 135                 140                 


Gln Ser Phe Ile His Ser Val Leu Pro Lys Ile Lys Gln Val Ala Asn 
145                 150                 155                 160 


Ile Val Lys Glu Lys Asn Leu Gln Val Glu Ile Glu Val Asp Gly Gly 
                165                 170                 175     


Val Asn Pro Glu Thr Ala Lys Leu Cys Val Glu Ala Gly Ala Asn Val 
            180                 185                 190         


Leu Val Ala Gly Ser Ala Ile Tyr Asn Gln Glu Asp Arg Ser Gln Ala 
        195                 200                 205             


Ile Ala Lys Ile Arg Asn 
    210                 


<210>  7
<211>  148
<212>  PRT
<213>  Bacillus methanolicus

<400>  7

Met Lys Val Ala Ile Ala Ser Asp His Gly Gly Val Asn Ile Arg Gln 
1               5                   10                  15      


Glu Ile Ile Asn Leu Leu Glu Glu Met Gly Ile Glu Tyr Glu Asp Phe 
            20                  25                  30          


Gly Cys Glu Cys Thr Thr Ser Val Asp Tyr Pro Asp Tyr Ala Leu Pro 
        35                  40                  45              


Val Ala Glu Lys Val Ala Asn Gly Glu Phe Asp Arg Gly Ile Leu Ile 
    50                  55                  60                  


Cys Gly Thr Gly Ile Gly Met Ser Ile Ala Ala Asn Lys Val Lys Gly 
65                  70                  75                  80  


Ile Arg Cys Ala Leu Val His Asp Val Phe Ser Ala Lys Ala Thr Arg 
                85                  90                  95      


Glu His Asn Asp Ser Asn Ile Leu Ala Met Gly Glu Arg Val Ile Gly 
            100                 105                 110         


Pro Gly Leu Ala Arg Glu Ile Ala Lys Thr Trp Leu Thr Thr Glu Phe 
        115                 120                 125             


Gln Gly Gly Arg His Ser Asn Arg Ile Glu Lys Ile Cys Lys Tyr Glu 
    130                 135                 140                 


Ser Val Asn Leu 
145             


<210>  8
<211>  668
<212>  PRT
<213>  Bacillus methanolicus

<400>  8

Met Phe Asp Lys Ile Asp Gln Leu Ser Ile Thr Ser Ile Arg Thr Leu 
1               5                   10                  15      


Ser Ile Asp Ala Ile Glu Lys Ala Asn Ser Gly His Pro Gly Met Pro 
            20                  25                  30          


Met Gly Ala Ala Pro Met Ala Tyr Thr Leu Trp Thr Arg Tyr Met Asn 
        35                  40                  45              


His Asn Pro Lys Asn Pro Asn Trp Phe Asn Arg Asp Arg Phe Val Leu 
    50                  55                  60                  


Ser Ala Gly His Gly Ser Met Leu Leu Tyr Ser Leu Leu His Leu Ser 
65                  70                  75                  80  


Gly Tyr Asp Val Thr Met Asp Asp Ile Lys Glu Phe Arg Gln Trp Gly 
                85                  90                  95      


Ser Lys Thr Pro Gly His Pro Glu Tyr Gly His Thr Pro Gly Val Glu 
            100                 105                 110         


Ala Thr Thr Gly Pro Leu Gly Gln Gly Ile Ala Met Ala Val Gly Ile 
        115                 120                 125             


Gly Met Ala Glu Arg His Leu Ala Ala Val Tyr Asn Lys Asp Asn Tyr 
    130                 135                 140                 


Gln Ile Ile Asn His Tyr Thr Tyr Ser Ile Cys Gly Asp Gly Asp Leu 
145                 150                 155                 160 


Met Glu Gly Val Ser Ala Glu Ala Ala Ser Leu Ala Gly His Leu Arg 
                165                 170                 175     


Leu Gly Arg Leu Ile Val Leu Tyr Asp Ser Asn Asp Ile Ser Leu Asp 
            180                 185                 190         


Gly Glu Leu Asn Arg Ser Phe Ser Glu Ser Val Glu Met Arg Phe Lys 
        195                 200                 205             


Ser Tyr Gly Trp Gln Tyr Ile Arg Val Glu Asp Gly Asn Asp Leu Glu 
    210                 215                 220                 


Ala Ile Ala Lys Ala Ile Glu Glu Ala Lys Gln Asp Glu Thr Arg Pro 
225                 230                 235                 240 


Thr Leu Ile Glu Val Lys Thr Ile Ile Gly Tyr Gly Ser Pro Asn Arg 
                245                 250                 255     


Ala Gly Thr Ser Asp Ile His Gly Ser Pro Leu Gly Ala Glu Glu Arg 
            260                 265                 270         


Lys Leu Thr Lys Glu Ala Tyr Lys Trp Thr Phe Glu Glu Asp Phe Tyr 
        275                 280                 285             


Val Pro Gln Glu Val Tyr Asp His Phe Lys Gln Asn Val Ile Glu Arg 
    290                 295                 300                 


Gly Glu Ala Lys Glu Gln Glu Trp Asn Glu Leu Phe Ala Gln Tyr Lys 
305                 310                 315                 320 


Lys Glu Tyr Pro Glu Leu Gly Lys Gln Leu Glu Gln Ala Ile Asn Gly 
                325                 330                 335     


Glu Leu Pro Glu Gly Trp Asp Lys Asp Ile Pro Val Tyr Glu Val Gly 
            340                 345                 350         


Lys Ser Ile Ala Ser Arg Ala Ser Ser Gly Glu Val Leu Asn Ala Ile 
        355                 360                 365             


Ala Lys Asn Leu Pro Ser Phe Ile Gly Gly Ser Ala Asp Leu Ala Ser 
    370                 375                 380                 


Ser Asn Lys Thr Thr Ile Lys Gly Ala Gly Asp Tyr Ser Pro Glu Ser 
385                 390                 395                 400 


Phe Glu Gly Lys Asn Ile Trp Phe Gly Val Arg Glu Phe Ala Met Gly 
                405                 410                 415     


Ala Ala Leu Asn Gly Met Ala Leu His Gly Gly Leu Lys Val Phe Gly 
            420                 425                 430         


Gly Thr Phe Phe Val Phe Ser Asp Tyr Leu Arg Pro Ala Ile Arg Leu 
        435                 440                 445             


Ala Ala Leu Met Lys Leu Pro Val Ile Tyr Val Phe Thr His Asp Ser 
    450                 455                 460                 


Ile Ala Val Gly Glu Asp Gly Pro Thr His Glu Pro Val Glu Gln Leu 
465                 470                 475                 480 


Ala Ser Leu Arg Ala Met Pro Asn Leu Ser Val Ile Arg Pro Ala Asp 
                485                 490                 495     


Gly Asn Glu Thr Ala Ala Ala Trp Arg Leu Ala Val Glu Ser Lys Asp 
            500                 505                 510         


Lys Pro Thr Ala Leu Val Leu Ser Arg Gln Asn Leu Pro Thr Leu Lys 
        515                 520                 525             


Gly Thr Ala Glu Thr Ala Tyr Asp Gly Val Ser Lys Gly Ala Tyr Val 
    530                 535                 540                 


Val Ser Pro Ala Asp Lys Asp Thr Pro Asp Ala Leu Leu Leu Ala Ser 
545                 550                 555                 560 


Gly Ser Glu Val Gly Leu Ala Val Glu Ala Gln Val Arg Leu Ala Glu 
                565                 570                 575     


Glu Gly Ile His Val Ser Val Ile Ser Met Pro Ser Phe Asp Arg Phe 
            580                 585                 590         


Glu Ala Gln Ser Lys Glu Tyr Lys Glu Ser Val Ile Pro Lys Asn Val 
        595                 600                 605             


Lys Lys Arg Leu Val Ile Glu Met Ala Ser Ser Leu Gly Leu His Arg 
    610                 615                 620                 


Tyr Ala Gly Asp Glu Gly Asp Val Leu Ala Ile Asp Gln Phe Gly Ala 
625                 630                 635                 640 


Ser Ala Pro Gly Glu Ile Ile Met Glu Glu Tyr Gly Phe Asn Val Asp 
                645                 650                 655     


Asn Val Val Ala Lys Val Lys Ala Leu Leu Glu Lys 
            660                 665             


<210>  9
<211>  284
<212>  PRT
<213>  Bacillus methanolicus

<400>  9

Met Pro Leu Val Ser Met Lys Asp Met Leu Asn His Gly Lys Glu Asn 
1               5                   10                  15      


Gly Tyr Ala Val Gly Gln Phe Asn Ile Asn Asn Leu Glu Phe Gly Gln 
            20                  25                  30          


Ala Ile Leu Gln Ala Ala Glu Glu Glu Lys Ser Pro Val Ile Ile Gly 
        35                  40                  45              


Val Ser Val Gly Ala Ala Asn Tyr Met Gly Gly Phe Lys Leu Ile Val 
    50                  55                  60                  


Asp Met Val Lys Ser Ser Met Asp Ser Tyr Asn Val Thr Val Pro Val 
65                  70                  75                  80  


Ala Ile His Leu Asp His Gly Pro Ser Leu Glu Lys Cys Val Gln Ala 
                85                  90                  95      


Ile His Ala Gly Phe Thr Ser Val Met Ile Asp Gly Ser His Leu Pro 
            100                 105                 110         


Leu Glu Glu Asn Ile Glu Leu Thr Lys Arg Val Val Glu Ile Ala His 
        115                 120                 125             


Ser Val Gly Val Ser Val Glu Ala Glu Leu Gly Arg Ile Gly Gly Gln 
    130                 135                 140                 


Glu Asp Asp Val Val Ala Glu Ser Phe Tyr Ala Ile Pro Ser Glu Cys 
145                 150                 155                 160 


Glu Gln Leu Val Arg Glu Thr Gly Val Asp Cys Phe Ala Pro Ala Leu 
                165                 170                 175     


Gly Ser Val His Gly Pro Tyr Lys Gly Glu Pro Lys Leu Gly Phe Asp 
            180                 185                 190         


Arg Met Glu Glu Ile Met Lys Leu Thr Gly Val Pro Leu Val Leu His 
        195                 200                 205             


Gly Gly Thr Gly Ile Pro Thr Lys Asp Ile Gln Lys Ala Ile Ser Leu 
    210                 215                 220                 


Gly Thr Ala Lys Ile Asn Val Asn Thr Glu Ser Gln Ile Ala Ala Thr 
225                 230                 235                 240 


Lys Ala Val Arg Glu Val Leu Asn Asn Asp Ala Lys Leu Phe Asp Pro 
                245                 250                 255     


Arg Lys Phe Leu Ala Pro Ala Arg Glu Ala Ile Lys Glu Thr Ile Lys 
            260                 265                 270         


Gly Lys Met Arg Glu Phe Gly Ser Ser Gly Lys Ala 
        275                 280                 


<210>  10
<211>  484
<212>  PRT
<213>  Bacillus methanolicus

<400>  10

Met Asp Ser Met Thr Phe Val Leu Phe Gly Ala Ser Gly Asp Leu Ala 
1               5                   10                  15      


Lys Arg Lys Ile Tyr Pro Ala Leu Tyr Asn Leu Phe Leu Asp Gln Lys 
            20                  25                  30          


Met Pro Gln Ser Phe Ser Val Ile Gly Leu Gly Arg Arg Asp Trp Ser 
        35                  40                  45              


Asp Asn Glu Tyr Gln Met Arg Val Glu Gln Ser Leu Lys Thr Phe Ser 
    50                  55                  60                  


Arg Arg Phe Val Ile Asp Ser Ile Lys Met Lys Glu Phe Leu Ser Ala 
65                  70                  75                  80  


Phe Arg Phe Phe Arg Leu Asp Val Asn Asn Glu Glu Gly Phe Arg Asp 
                85                  90                  95      


Leu Phe Glu Phe Val Lys Lys Arg Glu Glu Glu Leu Asn Ile Pro Glu 
            100                 105                 110         


Asn Arg Met Phe Tyr Leu Ser Val Ala Pro Glu Phe Ile Gly Val Ile 
        115                 120                 125             


Ala Ser Asn Ile Lys Lys Ser Gly Leu Gly Thr Thr Lys Gly Trp Lys 
    130                 135                 140                 


Arg Leu Val Ile Glu Lys Pro Phe Gly Asn Asp Leu Lys Ser Ala Gln 
145                 150                 155                 160 


Glu Leu Asn Glu Ser Leu Arg Asn Val Phe Glu Glu Asp Glu Ile Tyr 
                165                 170                 175     


Arg Ile Asp His Tyr Leu Gly Lys Pro Met Val Gln Asn Leu Glu Ala 
            180                 185                 190         


Leu Glu Phe Ala Asn Pro Val Leu Gln Ala Ile Trp Asn Asn Gln Tyr 
        195                 200                 205             


Ile Ala Asn Val Gln Ile Thr Ala Ser Glu Thr Val Gly Val Glu Glu 
    210                 215                 220                 


Arg Ala Gly Tyr Tyr Asp Gln Ala Gly Ala Ile Arg Asp Met Phe Gln 
225                 230                 235                 240 


Asn His Met Leu Gln Leu Leu Met Met Thr Ala Met Lys Met Pro Glu 
                245                 250                 255     


Arg Ile Ser Ser Lys Asp Ile Arg Asn Glu Lys Ile Lys Val Met Glu 
            260                 265                 270         


Tyr Leu Arg Pro Leu Leu Lys Glu Asp Val Ala Lys His Val Val Arg 
        275                 280                 285             


Gly Gln Tyr Gly Pro Gly Glu Ile Asn Gly Glu Pro Val Val Gly Tyr 
    290                 295                 300                 


Arg Glu Glu Pro Gly Val Asp Ala Ser Ser Thr Thr Asp Thr Phe Val 
305                 310                 315                 320 


Ala Ala Arg Leu Trp Ile Asp Val Pro Phe Trp Ser Gly Val Pro Phe 
                325                 330                 335     


Tyr Ile Arg Thr Gly Lys Arg Met Arg Glu Lys Cys Thr Lys Ile Val 
            340                 345                 350         


Ile Glu Phe Lys Asn Pro Leu Lys Asp Leu Tyr Ile Thr Asp Asn Met 
        355                 360                 365             


Lys Thr Val Pro Asn Leu Leu Val Ile Glu Ile Asn Pro Asn Glu Arg 
    370                 375                 380                 


Val Ser Phe Gln Leu Asn Ser Lys Asn Pro Ile Asp Gly Lys Ile Glu 
385                 390                 395                 400 


Pro Val His Val Asn Phe Ser Ala Ser Gln Lys Asp Ala Pro Glu Ala 
                405                 410                 415     


Tyr Glu Ile Leu Leu Tyr Asp Ala Leu Arg Gly Asp Ser Thr Tyr Phe 
            420                 425                 430         


Ala His Trp Lys Glu Val Glu Leu Ser Trp Lys Trp Val Gln Pro Ile 
        435                 440                 445             


Leu Glu Ala Phe Glu Glu Asn Ile Leu Pro Leu His His Tyr Thr Ser 
    450                 455                 460                 


Gly Ser Met Gly Pro Glu Ala Ser His Lys Leu Leu Glu Glu Asp Gly 
465                 470                 475                 480 


Phe Asn Trp Trp 
                


