                         SEQUENCE LISTING

<110>  Technische Universitaet Muenchen
 
<120>  Means and methods for site-specific protein modification

<130>  TUM16418PCT

<150>  EP 18181497.1
<151>  2018-07-03

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  148
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Pro Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asp Val Lys Pro Thr Asp Val Gly Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Lys Thr Gly Val Trp Glu Lys Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  2
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Srt2A

<400>  2

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Cys Phe His Asp Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Pro 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Ile Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Lys Val His Pro Asn Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Glu Gly Lys Asp Lys Gln Leu Thr Leu Val Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  3
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Srt5M

<400>  3

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Thr Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  4
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Srt7M

<400>  4

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Ser Phe Ala Lys Glu 
        35                  40                  45              


Asn Gln Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Thr Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  5
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mSrt2A

<400>  5

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asn Arg Gly Val Cys Phe His Lys Glu 
        35                  40                  45              


Asn Gln Ser Leu Asp Asp Gln Asn Ile Ser Ile Ala Gly His Thr Phe 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Lys Ala Ala Lys Pro 
65                  70                  75                  80  


Gly Ser Met Val Tyr Phe Lys Val Gly Asn Glu Thr Arg Ile Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Lys Val His Pro Asn Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Glu Gly Lys Asp Lys Gln Leu Thr Leu Val Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Glu Glu Thr Gly Val Trp Glu Ser Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  6
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Srt4S

<400>  6

Met Gln Ala Lys Pro Gln Ile Pro Lys Asp Lys Ser Lys Val Ala Gly 
1               5                   10                  15      


Tyr Ile Glu Ile Pro Asp Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly 
            20                  25                  30          


Pro Ala Thr Arg Glu Gln Leu Asp Arg Gly Val Cys Phe Val Glu Glu 
        35                  40                  45              


Asn Glu Ser Leu Asp Asp Gln Asn Ile Ser Ile Thr Gly His Thr Ala 
    50                  55                  60                  


Ile Asp Arg Pro Asn Tyr Gln Phe Thr Asn Leu Arg Ala Ala Lys Lys 
65                  70                  75                  80  


Gly Ser Met Val Tyr Leu Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys 
                85                  90                  95      


Met Thr Ser Ile Arg Asn Val Lys Pro Thr Ala Val Glu Val Leu Asp 
            100                 105                 110         


Glu Gln Lys Gly Lys Asp Lys Gln Leu Thr Leu Val Thr Cys Asp Asp 
        115                 120                 125             


Tyr Asn Phe Glu Thr Gly Val Trp Glu Thr Arg Lys Ile Phe Val Ala 
    130                 135                 140                 


Thr Glu Val Lys 
145             


<210>  7
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  7

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Leu Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Cys Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  8
<211>  71
<212>  RNA
<213>  Methanosarcina barkeri


<220>
<221>  Anticodon
<222>  (31)..(33)
<223>  n can be A, U, G or C

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  n is a, c, g, or u

<400>  8
gggaaccuga ucauguagau cgaauggacu nnnauccguu cagccggguu agauucccgg       60

gguuuccgcc a                                                            71


<210>  9
<211>  68
<212>  RNA
<213>  Methanosarcina mazeii


<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  n is a, c, g, or u

<400>  9
ggaaaccuga ucauguagau cgaacggacu nnnauccguu cagccggguu agauucccgg       60

gguuuccg                                                                68


<210>  10
<211>  419
<212>  PRT
<213>  Methanosarcina barkeri

<400>  10

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Gln Phe Ser Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  11
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA synthetase AzGK

<400>  11

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Gly Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Gly Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Leu Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Phe Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  12
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA synthetase GK

<400>  12

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Gly Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Gly Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Met Ala Pro Thr Ile Phe Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Phe Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  13
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  pyrrolysyl tRNA synthetase GGK

<400>  13

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Gly Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Gly Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Met Ala Pro Thr Ile Phe Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Asn Phe Phe Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  14
<211>  72
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Methanosarcina barkeri tRNACUA

<400>  14
gggaaccuga ucauguagau cgaauggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguuuccgc ca                                                           72


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  15

Leu Pro Xaa Thr Gly 
1               5   


<210>  16
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  16

Leu Leu Pro Xaa Thr Gly 
1               5       


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  17

Leu Ala Xaa Thr Gly 
1               5   


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  18

Leu Leu Ala Xaa Thr Gly 
1               5       


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  19

Leu Pro Xaa Ser Gly 
1               5   


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sortase recognition motif


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  20

Leu Leu Pro Xaa Ser Gly 
1               5       


<210>  21
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AzGGKRS

<400>  21

Met Asp Lys Lys Pro Leu Asp Val Leu Ile Ser Ala Thr Gly Leu Trp 
1               5                   10                  15      


Met Ser Arg Thr Gly Thr Leu His Lys Ile Lys His His Glu Val Ser 
            20                  25                  30          


Arg Ser Lys Ile Tyr Ile Glu Met Ala Cys Gly Asp His Leu Val Val 
        35                  40                  45              


Asn Asn Ser Arg Ser Cys Arg Thr Ala Arg Ala Phe Arg His His Lys 
    50                  55                  60                  


Tyr Arg Lys Thr Cys Lys Arg Cys Arg Val Ser Asp Glu Asp Ile Asn 
65                  70                  75                  80  


Asn Phe Leu Thr Arg Ser Thr Glu Ser Lys Asn Ser Val Lys Val Arg 
                85                  90                  95      


Val Val Ser Ala Pro Lys Val Lys Lys Ala Met Pro Lys Ser Val Ser 
            100                 105                 110         


Arg Ala Pro Lys Pro Leu Glu Asn Ser Val Ser Ala Lys Ala Ser Thr 
        115                 120                 125             


Asn Thr Ser Arg Ser Val Pro Ser Pro Ala Lys Ser Thr Pro Asn Ser 
    130                 135                 140                 


Ser Val Pro Ala Ser Ala Pro Ala Pro Ser Leu Thr Arg Ser Gln Leu 
145                 150                 155                 160 


Asp Arg Val Glu Ala Leu Leu Ser Pro Glu Asp Lys Ile Ser Leu Asn 
                165                 170                 175     


Met Ala Lys Pro Phe Arg Glu Leu Glu Pro Glu Leu Val Thr Arg Arg 
            180                 185                 190         


Lys Asn Asp Phe Gln Arg Leu Tyr Thr Asn Asp Arg Glu Asp Tyr Leu 
        195                 200                 205             


Gly Lys Leu Glu Arg Asp Ile Thr Lys Phe Phe Val Asp Arg Gly Phe 
    210                 215                 220                 


Leu Glu Ile Lys Ser Pro Ile Leu Ile Pro Ala Glu Tyr Val Glu Arg 
225                 230                 235                 240 


Met Gly Ile Asn Asn Asp Thr Glu Leu Ser Lys Gln Ile Phe Arg Val 
                245                 250                 255     


Asp Lys Asn Leu Cys Leu Arg Pro Met Leu Ala Pro Thr Leu Tyr Asn 
            260                 265                 270         


Tyr Ala Arg Lys Leu Asp Arg Ile Leu Pro Gly Pro Ile Lys Ile Phe 
        275                 280                 285             


Glu Val Gly Pro Cys Tyr Arg Lys Glu Ser Asp Gly Lys Glu His Leu 
    290                 295                 300                 


Glu Glu Phe Thr Met Val Gln Phe Ser Gln Met Gly Ser Gly Cys Thr 
305                 310                 315                 320 


Arg Glu Asn Leu Glu Ala Leu Ile Lys Glu Phe Leu Asp Tyr Leu Glu 
                325                 330                 335     


Ile Asp Phe Glu Ile Val Gly Asp Ser Cys Met Val Tyr Gly Asp Thr 
            340                 345                 350         


Leu Asp Ile Met His Gly Asp Leu Glu Leu Ser Ser Ala Val Val Gly 
        355                 360                 365             


Pro Val Ser Leu Asp Arg Glu Trp Gly Ile Asp Lys Pro Trp Ile Gly 
    370                 375                 380                 


Ala Gly Phe Gly Leu Glu Arg Leu Leu Lys Val Met His Gly Phe Lys 
385                 390                 395                 400 


Asn Ile Lys Arg Ala Ser Arg Ser Glu Ser Tyr Tyr Asn Gly Ile Ser 
                405                 410                 415     


Thr Asn Leu 
            


<210>  22
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sfGFP-N150TAG-His6


<220>
<221>  misc_feature
<222>  (150)..(150)
<223>  Xaa can be any unnatural amino acid

<400>  22

Met Pro Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Xaa Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Gly 
225                 230                 235                 240 


Ser His His His His His His 
                245         


<210>  23
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K6TAG-His6


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any unnatural amino acid

<400>  23

Met Gln Ile Phe Val Xaa Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  24
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K11TAG-His6


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  Xaa can be any unnatural amino acid

<400>  24

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Xaa Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  25
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K27TAG-His6


<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Xaa can be any unnatural amino acid

<400>  25

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Xaa Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  26
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K29TAG-His6


<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Xaa can be any unnatural amino acid

<400>  26

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Xaa Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  27
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K33TAG-His6


<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any unnatural amino acid

<400>  27

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Xaa Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  28
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K48TAG-His6


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  Xaa can be any unnatural amino acid

<400>  28

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Xaa 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  29
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ubiquitin-K63TAG-His6


<220>
<221>  misc_feature
<222>  (63)..(63)
<223>  Xaa can be any unnatural amino acid

<400>  29

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Xaa Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly His His His His 
65                  70                  75                  80  


His His 
        


<210>  30
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type ubiquitin

<400>  30

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly 
65                  70                  75      


<210>  31
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ub(PT)

<400>  31

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Pro Leu Thr Gly Gly 
65                  70                  75      


<210>  32
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ub(LPT)

<400>  32

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Leu Pro Leu Thr Gly Gly 
65                  70                  75          


<210>  33
<211>  76
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ub(AT)

<400>  33

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Ala Leu Thr Gly Gly 
65                  70                  75      


<210>  34
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ub(LAT)

<400>  34

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Leu Ala Leu Thr Gly Gly 
65                  70                  75          


<210>  35
<211>  90
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-TEV-Ub(AT)

<400>  35

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Gln 
1               5                   10                  15      


Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
            20                  25                  30          


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
        35                  40                  45              


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu 
    50                  55                  60                  


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr 
65                  70                  75                  80  


Leu His Leu Val Leu Ala Leu Thr Gly Gly 
                85                  90  


<210>  36
<211>  91
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV-Ub(LAT)

<400>  36

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Gln 
1               5                   10                  15      


Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu 
            20                  25                  30          


Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu 
        35                  40                  45              


Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu 
    50                  55                  60                  


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr 
65                  70                  75                  80  


Leu His Leu Val Leu Leu Ala Leu Thr Gly Gly 
                85                  90      


<210>  37
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6-TEV-SUMO(AT)

<400>  37

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Ser 
1               5                   10                  15      


Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu Gly Asp Lys Lys Glu 
            20                  25                  30          


Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser Glu Ile 
        35                  40                  45              


His Phe Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys Glu Ser 
    50                  55                  60                  


Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe Leu Phe 
65                  70                  75                  80  


Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu Gly Met 
                85                  90                  95      


Glu Glu Glu Asp Val Ile Glu Val Tyr Leu Ala Gln Thr Gly Gly 
            100                 105                 110     


<210>  38
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6-TEV-SUMO(LAT)

<400>  38

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Ser 
1               5                   10                  15      


Asp Gln Glu Ala Lys Pro Ser Thr Glu Asp Leu Gly Asp Lys Lys Glu 
            20                  25                  30          


Gly Glu Tyr Ile Lys Leu Lys Val Ile Gly Gln Asp Ser Ser Glu Ile 
        35                  40                  45              


His Phe Lys Val Lys Met Thr Thr His Leu Lys Lys Leu Lys Glu Ser 
    50                  55                  60                  


Tyr Cys Gln Arg Gln Gly Val Pro Met Asn Ser Leu Arg Phe Leu Phe 
65                  70                  75                  80  


Glu Gly Gln Arg Ile Ala Asp Asn His Thr Pro Lys Glu Leu Gly Met 
                85                  90                  95      


Glu Glu Glu Asp Val Ile Glu Val Tyr Leu Leu Ala Gln Thr Gly Gly 
            100                 105                 110         


<210>  39
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCNA-K164TAG-CPD-His6


<220>
<221>  misc_feature
<222>  (164)..(164)
<223>  Xaa can be any unnatural aminom acid

<400>  39

Met Phe Glu Ala Arg Leu Val Gln Gly Ser Ile Leu Lys Lys Val Leu 
1               5                   10                  15      


Glu Ala Leu Lys Asp Leu Ile Asn Glu Ala Cys Trp Asp Ile Ser Ser 
            20                  25                  30          


Ser Gly Val Asn Leu Gln Ser Met Asp Ser Ser His Val Ser Leu Val 
        35                  40                  45              


Gln Leu Thr Leu Arg Ser Glu Gly Phe Asp Thr Tyr Arg Cys Asp Arg 
    50                  55                  60                  


Asn Leu Ala Met Gly Val Asn Leu Thr Ser Met Ser Lys Ile Leu Lys 
65                  70                  75                  80  


Cys Ala Gly Asn Glu Asp Ile Ile Thr Leu Arg Ala Glu Asp Asn Ala 
                85                  90                  95      


Asp Thr Leu Ala Leu Val Phe Glu Ala Pro Asn Gln Glu Lys Val Ser 
            100                 105                 110         


Asp Tyr Glu Met Lys Leu Met Asp Leu Asp Val Glu Gln Leu Gly Ile 
        115                 120                 125             


Pro Glu Gln Glu Tyr Ser Cys Val Val Lys Met Pro Ser Gly Glu Phe 
    130                 135                 140                 


Ala Arg Ile Cys Arg Asp Leu Ser His Ile Gly Asp Ala Val Val Ile 
145                 150                 155                 160 


Ser Cys Ala Xaa Asp Gly Val Lys Phe Ser Ala Ser Gly Glu Leu Gly 
                165                 170                 175     


Asn Gly Asn Ile Lys Leu Ser Gln Thr Ser Asn Val Asp Lys Glu Glu 
            180                 185                 190         


Glu Ala Val Thr Ile Glu Met Asn Glu Pro Val Gln Leu Thr Phe Ala 
        195                 200                 205             


Leu Arg Tyr Leu Asn Phe Phe Thr Lys Ala Thr Pro Leu Ser Ser Thr 
    210                 215                 220                 


Val Thr Leu Ser Met Ser Ala Asp Val Pro Leu Val Val Glu Tyr Lys 
225                 230                 235                 240 


Ile Ala Asp Met Gly His Leu Lys Tyr Tyr Leu Ala Pro Lys Ile Glu 
                245                 250                 255     


Asp Glu Glu Gly Ser Leu Ala Gly Gly Lys Ile Leu His Asn Gln Asn 
            260                 265                 270         


Val Asn Ser Trp Gly Pro Ile Thr Val Thr Pro Thr Thr Asp Gly Gly 
        275                 280                 285             


Glu Thr Arg Phe Asp Gly Gln Ile Ile Val Gln Met Glu Asn Asp Pro 
    290                 295                 300                 


Val Val Ala Lys Ala Ala Ala Asn Leu Ala Gly Lys His Ala Glu Ser 
305                 310                 315                 320 


Ser Val Val Val Gln Leu Asp Ser Asp Gly Asn Tyr Arg Val Val Tyr 
                325                 330                 335     


Gly Asp Pro Ser Lys Leu Asp Gly Lys Leu Arg Trp Gln Leu Val Gly 
            340                 345                 350         


His Gly Arg Asp His Ser Glu Thr Asn Asn Thr Arg Leu Ser Gly Tyr 
        355                 360                 365             


Ser Ala Asp Glu Leu Ala Val Lys Leu Ala Lys Phe Gln Gln Ser Phe 
    370                 375                 380                 


Asn Gln Ala Glu Asn Ile Asn Asn Lys Pro Asp His Ile Ser Ile Val 
385                 390                 395                 400 


Gly Cys Ser Leu Val Ser Asp Asp Lys Gln Lys Gly Phe Gly His Gln 
                405                 410                 415     


Phe Ile Asn Ala Met Asp Ala Asn Gly Leu Arg Val Asp Val Ser Val 
            420                 425                 430         


Arg Ser Ser Glu Leu Ala Val Asp Glu Ala Gly Arg Lys His Thr Lys 
        435                 440                 445             


Asp Ala Asn Gly Asp Trp Val Gln Lys Ala Glu Asn Asn Lys Val Ser 
    450                 455                 460                 


Ile Ser Trp Asp Ala Gln Gly His His His His His His 
465                 470                 475         


<210>  40
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PCNA-K164TAG (after CPD cleavage)


<220>
<221>  misc_feature
<222>  (164)..(164)
<223>  Xaa can be any unnatural amino acid

<400>  40

Met Phe Glu Ala Arg Leu Val Gln Gly Ser Ile Leu Lys Lys Val Leu 
1               5                   10                  15      


Glu Ala Leu Lys Asp Leu Ile Asn Glu Ala Cys Trp Asp Ile Ser Ser 
            20                  25                  30          


Ser Gly Val Asn Leu Gln Ser Met Asp Ser Ser His Val Ser Leu Val 
        35                  40                  45              


Gln Leu Thr Leu Arg Ser Glu Gly Phe Asp Thr Tyr Arg Cys Asp Arg 
    50                  55                  60                  


Asn Leu Ala Met Gly Val Asn Leu Thr Ser Met Ser Lys Ile Leu Lys 
65                  70                  75                  80  


Cys Ala Gly Asn Glu Asp Ile Ile Thr Leu Arg Ala Glu Asp Asn Ala 
                85                  90                  95      


Asp Thr Leu Ala Leu Val Phe Glu Ala Pro Asn Gln Glu Lys Val Ser 
            100                 105                 110         


Asp Tyr Glu Met Lys Leu Met Asp Leu Asp Val Glu Gln Leu Gly Ile 
        115                 120                 125             


Pro Glu Gln Glu Tyr Ser Cys Val Val Lys Met Pro Ser Gly Glu Phe 
    130                 135                 140                 


Ala Arg Ile Cys Arg Asp Leu Ser His Ile Gly Asp Ala Val Val Ile 
145                 150                 155                 160 


Ser Cys Ala Xaa Asp Gly Val Lys Phe Ser Ala Ser Gly Glu Leu Gly 
                165                 170                 175     


Asn Gly Asn Ile Lys Leu Ser Gln Thr Ser Asn Val Asp Lys Glu Glu 
            180                 185                 190         


Glu Ala Val Thr Ile Glu Met Asn Glu Pro Val Gln Leu Thr Phe Ala 
        195                 200                 205             


Leu Arg Tyr Leu Asn Phe Phe Thr Lys Ala Thr Pro Leu Ser Ser Thr 
    210                 215                 220                 


Val Thr Leu Ser Met Ser Ala Asp Val Pro Leu Val Val Glu Tyr Lys 
225                 230                 235                 240 


Ile Ala Asp Met Gly His Leu Lys Tyr Tyr Leu Ala Pro Lys Ile Glu 
                245                 250                 255     


Asp Glu Glu Gly Ser Leu 
            260         


<210>  41
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Subtiligase (mature form)

<400>  41

Ala Gln Ser Val Pro Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Phe Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asp Asn Ser Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Gly Ala Asp Gly Ser Gly Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu 
            100                 105                 110         


Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Leu Ala Leu Gly Gly 
        115                 120                 125             


Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala 
    130                 135                 140                 


Ser Gly Val Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 
145                 150                 155                 160 


Ser Ser Ser Thr Val Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 
                165                 170                 175     


Val Gly Ala Val Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val 
            180                 185                 190         


Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             


Leu Pro Gly Asn Arg Tyr Gly Ala Tyr Ser Gly Thr Cys Met Ala Ser 
    210                 215                 220                 


Ala His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn 
225                 230                 235                 240 


Trp Thr Asn Thr Gln Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 
                245                 250                 255     


Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ala Gln 
        275 


<210>  42
<211>  206
<212>  PRT
<213>  Staphylococcus aureus

<400>  42

Met Lys Lys Trp Thr Asn Arg Leu Met Thr Ile Ala Gly Val Val Leu 
1               5                   10                  15      


Ile Leu Val Ala Ala Tyr Leu Phe Ala Lys Pro His Ile Asp Asn Tyr 
            20                  25                  30          


Leu His Asp Lys Asp Lys Asp Glu Lys Ile Glu Gln Tyr Asp Lys Asn 
        35                  40                  45              


Val Lys Glu Gln Ala Ser Lys Asp Lys Lys Gln Gln Ala Lys Pro Gln 
    50                  55                  60                  


Ile Pro Lys Asp Lys Ser Lys Val Ala Gly Tyr Ile Glu Ile Pro Asp 
65                  70                  75                  80  


Ala Asp Ile Lys Glu Pro Val Tyr Pro Gly Pro Ala Thr Pro Glu Gln 
                85                  90                  95      


Leu Asn Arg Gly Val Ser Phe Ala Glu Glu Asn Glu Ser Leu Asp Asp 
            100                 105                 110         


Gln Asn Ile Ser Ile Ala Gly His Thr Phe Ile Asp Arg Pro Asn Tyr 
        115                 120                 125             


Gln Phe Thr Asn Leu Lys Ala Ala Lys Lys Gly Ser Met Val Tyr Phe 
    130                 135                 140                 


Lys Val Gly Asn Glu Thr Arg Lys Tyr Lys Met Thr Ser Ile Arg Asp 
145                 150                 155                 160 


Val Lys Pro Thr Asp Val Gly Val Leu Asp Glu Gln Lys Gly Lys Asp 
                165                 170                 175     


Lys Gln Leu Thr Leu Ile Thr Cys Asp Asp Tyr Asn Glu Lys Thr Gly 
            180                 185                 190         


Val Trp Glu Lys Arg Lys Ile Phe Val Ala Thr Glu Val Lys 
        195                 200                 205     


<210>  43
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  His6-TEV-G-SUMO2

<400>  43

Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Gly Ala 
1               5                   10                  15      


Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp His Ile 
            20                  25                  30          


Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe Lys Ile 
        35                  40                  45              


Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys Glu Arg 
    50                  55                  60                  


Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly Gln Pro 
65                  70                  75                  80  


Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp Glu Asp 
                85                  90                  95      


Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly 
            100                 105         


<210>  44
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K11TAG-His6


<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  X can be any unnatural amino acid

<400>  44

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Xaa Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  45
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K21TAG-His6


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Xaa can be any unnatural amino acid

<400>  45

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Xaa Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  46
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K33TAG-His6


<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any unnatural amino acid

<400>  46

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Xaa Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  47
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K35TAG-His6


<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  Xaa can be any unnatural amino acid

<400>  47

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Xaa Arg His Thr Pro Leu Ser Lys Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  48
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K42TAG-His6


<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  Xaa can be any unnatural amino acid

<400>  48

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Lys Arg His Thr Pro Leu Ser Xaa Leu Met Lys Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  49
<211>  99
<212>  PRT
<213>  Artificial

<220>
<223>  SUMO2-K45TAG-His6


<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  Xaa can be any unnatural amino acid

<400>  49

Met Ala Asp Glu Lys Pro Lys Glu Gly Val Lys Thr Glu Asn Asn Asp 
1               5                   10                  15      


His Ile Asn Leu Lys Val Ala Gly Gln Asp Gly Ser Val Val Gln Phe 
            20                  25                  30          


Lys Ile Lys Arg His Thr Pro Leu Ser Lys Leu Met Xaa Ala Tyr Cys 
        35                  40                  45              


Glu Arg Gln Gly Leu Ser Met Arg Gln Ile Arg Phe Arg Phe Asp Gly 
    50                  55                  60                  


Gln Pro Ile Asn Glu Thr Asp Thr Pro Ala Gln Leu Glu Met Glu Asp 
65                  70                  75                  80  


Glu Asp Thr Ile Asp Val Phe Gln Gln Gln Thr Gly Gly His His His 
                85                  90                  95      


His His His 
            


<210>  50
<211>  83
<212>  PRT
<213>  Artificial

<220>
<223>  Ub-K48TAG-LLPLTGG-His6


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  Xaa can be any amino acid

<400>  50

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Xaa 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Leu Pro Leu Thr Gly Gly His His His 
65                  70                  75                  80  


His His His 
            


<210>  51
<211>  83
<212>  PRT
<213>  Artificial

<220>
<223>  Ub-K63TAG-LLALTGG-His6


<220>
<221>  misc_feature
<222>  (63)..(63)
<223>  Xaa can be any unnatural amino acid

<400>  51

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Xaa Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Leu Ala Leu Thr Gly Gly His His His 
65                  70                  75                  80  


His His His 
            


<210>  52
<211>  77
<212>  PRT
<213>  Artificial

<220>
<223>  Ub(LPS)

<400>  52

Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys 
        35                  40                  45              


Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu 
    50                  55                  60                  


Ser Thr Leu His Leu Val Leu Leu Pro Leu Ser Gly Gly 
65                  70                  75          


