                         SEQUENCE LISTING

<110>  UNIVERSITE LAVAL
       THE UNIVERSITY OF MANITOBA
 
<120>  THE EBOLA VIRUS GLYCOPROTEIN AS A TOOL TO STIMULATE AN IMMUNE 
       RESPONSE

<130>  16100-001-PCT

<140>  PCT/CA2019/050975
<141>  2019-01-15

<150>  62/697,695
<151>  2018-07-13

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ebola glycoprotein gene sequence of Makona B6 variant

<400>  1
atgggtgtta caggaatatt gcagttacct cgtgatcgat tcaagaggac atcattcttt       60

ctttgggtaa ttatcctttt ccaaagaaca ttttccatcc cgcttggagt tatccacaat      120

agtacattac aggttagtga tgtcgacaaa ctagtttgtc gtgacaaact gtcatccaca      180

aatcaattga gatcagttgg actgaatctc gaggggaatg gagtggcaac tgacgtgcca      240

tctgtgacta aaagatgggg cttcaggtcc ggtgtcccac caaaggtggt caattatgaa      300

gctggtgaat gggctgaaaa ctgctacaat cttgaaatca aaaaacctga cgggagtgag      360

tgtctaccag cagcgccaga cgggattcgg ggcttccccc ggtgccggta tgtgcacaaa      420

gtatcaggaa cgggaccatg tgccggagac tttgccttcc acaaagaggg tgctttcttc      480

ctgtatgatc gacttgcttc cacagttatc taccgaggaa cgactttcgc tgaaggtgtc      540

gttgcatttc tgatactgcc ccaagctaag aaggacttct tcagctcaca ccccttgaga      600

gagctggtca atgcaacgga ggacccgtcg agtggctatt attctaccac aattagatat      660

caggctaccg gttttggaac taatgagaca gagtacttgt tcgaggttga caattcgacc      720

tacgtccaac ttgaatcaag attcacacca cagtttctgc tccagctgaa tgagacaata      780

tatgcaagtg ggaagaggag caacaccacg ggaaaactaa tttggaaggt caaccccgaa      840

attgatacaa caatcgggga gtgggccttc agggaaacta aaaaaaacct cactagaaaa      900

attcgcagtg aagagttgtc tttcacagct gtatcaaacg gacccaaaaa catcagtggt      960

cagagtccgg cgcgaacttc ttccgaccca gagaccaaca caacaaatga agaccacaaa     1020

atcatggctt cagaaaattc ctctgcaatg gttcaagtgc acagtcaagg aaggaaagct     1080

gcagtgtcgc atctgacaac ccttgccaca atctccacga gtcctcaacc tcccacaacc     1140

aaaacaggtc cggacaacag cacccataat acacccgtgt ataaacttga catctctgag     1200

gcaactcaag ttggacaaca tcaccgtaga gcagacaacg acagcacagc ctccgacact     1260

ccccccgcca cgaccgcagc cggaccctta aaagcagaga acaccaacac gagtaagagc     1320

gctgactccc tggacctcgc caccacgaca agcccccaaa actacagcga gactgctggc     1380

aacaacaaca ctcatcacca agataccgga gaagagagtg ccagcagcgg gaagctaggc     1440

ttaattacca atactattgc tggagtagca ggactgatca caggcgggag aaggactcga     1500

agagaagtaa ttgtcaatgc tcaacccaaa tgcaacccca atttacatta ctggactact     1560

caggatgaag gtgctgcaat cggattggcc tggataccat atttcgggcc agcagccgaa     1620

ggaatttaca tagaggggct aatgcacaac caagatggtt taatctgtgg gttgaggcag     1680

ctggccaacg aaacgactca agctctccaa ctgttcctga gagccacaac tgagctgcga     1740

accttttcaa tcctcaaccg taaggcaatt gacttcctgc tgcagcgatg gggtggcaca     1800

tgccacattt tgggaccgga ctgctgtatc gaaccacatg attggaccaa gaacataaca     1860

gacaaaattg atcagattat tcatgatttt gttgataaaa cccttccgga ccagggggac     1920

aatgacaatt ggtggacagg atggagacaa tggataccgg caggtattgg agttacaggt     1980

gttataattg cagttatcgc tttattctgt atatgcaaat ttgtctttta g              2031


<210>  2
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ebola Glycoprotein sequence of Makona B6 variant

<400>  2

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Val Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Leu Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Ser Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Ala Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Arg Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Leu Ser Phe Thr Ala Val Ser Asn Gly Pro Lys Asn Ile Ser Gly 
305                 310                 315                 320 


Gln Ser Pro Ala Arg Thr Ser Ser Asp Pro Glu Thr Asn Thr Thr Asn 
                325                 330                 335     


Glu Asp His Lys Ile Met Ala Ser Glu Asn Ser Ser Ala Met Val Gln 
            340                 345                 350         


Val His Ser Gln Gly Arg Lys Ala Ala Val Ser His Leu Thr Thr Leu 
        355                 360                 365             


Ala Thr Ile Ser Thr Ser Pro Gln Pro Pro Thr Thr Lys Thr Gly Pro 
    370                 375                 380                 


Asp Asn Ser Thr His Asn Thr Pro Val Tyr Lys Leu Asp Ile Ser Glu 
385                 390                 395                 400 


Ala Thr Gln Val Gly Gln His His Arg Arg Ala Asp Asn Asp Ser Thr 
                405                 410                 415     


Ala Ser Asp Thr Pro Pro Ala Thr Thr Ala Ala Gly Pro Leu Lys Ala 
            420                 425                 430         


Glu Asn Thr Asn Thr Ser Lys Ser Ala Asp Ser Leu Asp Leu Ala Thr 
        435                 440                 445             


Thr Thr Ser Pro Gln Asn Tyr Ser Glu Thr Ala Gly Asn Asn Asn Thr 
    450                 455                 460                 


His His Gln Asp Thr Gly Glu Glu Ser Ala Ser Ser Gly Lys Leu Gly 
465                 470                 475                 480 


Leu Ile Thr Asn Thr Ile Ala Gly Val Ala Gly Leu Ile Thr Gly Gly 
                485                 490                 495     


Arg Arg Thr Arg Arg Glu Val Ile Val Asn Ala Gln Pro Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Ile 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asp Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Val Asp Lys Thr Leu Pro Asp Gln Gly Asp 
625                 630                 635                 640 


Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys 
            660                 665                 670         


Lys Phe Val Phe 
        675     


<210>  3
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike glycoprotein (Zaire ebolavirus)

<400>  3

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Pro Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Leu Ser Phe Thr Val Val Ser Asn Gly Ala Lys Asn Ile Ser Gly 
305                 310                 315                 320 


Gln Ser Pro Ala Arg Thr Ser Ser Asp Pro Gly Thr Asn Thr Thr Thr 
                325                 330                 335     


Glu Asp His Lys Ile Met Ala Ser Glu Asn Ser Ser Ala Met Val Gln 
            340                 345                 350         


Val His Ser Gln Gly Arg Glu Ala Ala Val Ser His Leu Thr Thr Leu 
        355                 360                 365             


Ala Thr Ile Ser Thr Ser Pro Gln Ser Leu Thr Thr Lys Pro Gly Pro 
    370                 375                 380                 


Asp Asn Ser Thr His Asn Thr Pro Val Tyr Lys Leu Asp Ile Ser Glu 
385                 390                 395                 400 


Ala Thr Gln Val Glu Gln His His Arg Arg Thr Asp Asn Asp Ser Thr 
                405                 410                 415     


Ala Ser Asp Thr Pro Ser Ala Thr Thr Ala Ala Gly Pro Pro Lys Ala 
            420                 425                 430         


Glu Asn Thr Asn Thr Ser Lys Ser Thr Asp Phe Leu Asp Pro Ala Thr 
        435                 440                 445             


Thr Thr Ser Pro Gln Asn His Ser Glu Thr Ala Gly Asn Asn Asn Thr 
    450                 455                 460                 


His His Gln Asp Thr Gly Glu Glu Ser Ala Ser Ser Gly Lys Leu Gly 
465                 470                 475                 480 


Leu Ile Thr Asn Thr Ile Ala Gly Val Ala Gly Leu Ile Thr Gly Gly 
                485                 490                 495     


Arg Arg Thr Arg Arg Glu Ala Ile Val Asn Ala Gln Pro Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Ile 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asp Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Val Asp Lys Thr Leu Pro Asp Gln Gly Asp 
625                 630                 635                 640 


Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys 
            660                 665                 670         


Lys Phe Val Phe 
        675     


<210>  4
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike glycoprotein (Sudan ebolavirus)

<400>  4

Met Gly Gly Leu Ser Leu Leu Gln Leu Pro Arg Asp Lys Phe Arg Lys 
1               5                   10                  15      


Ser Ser Phe Phe Val Trp Val Ile Ile Leu Phe Gln Lys Ala Phe Ser 
            20                  25                  30          


Met Pro Leu Gly Val Val Thr Asn Ser Thr Leu Glu Val Thr Glu Ile 
        35                  40                  45              


Asp Gln Leu Val Cys Lys Asp His Leu Ala Ser Thr Asp Gln Leu Lys 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Ser Gly Val Ser Thr Asp Ile Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Ser Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Pro Pro Pro Asp Gly 
        115                 120                 125             


Val Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Ala Gln Gly Thr 
    130                 135                 140                 


Gly Pro Cys Pro Gly Asp Tyr Ala Phe His Lys Asp Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Val Asn Phe 
                165                 170                 175     


Ala Glu Gly Val Ile Ala Phe Leu Ile Leu Ala Lys Pro Lys Glu Thr 
            180                 185                 190         


Phe Leu Gln Ser Pro Pro Ile Arg Glu Ala Val Asn Tyr Thr Glu Asn 
        195                 200                 205             


Thr Ser Ser Tyr Tyr Ala Thr Ser Tyr Leu Glu Tyr Glu Ile Glu Asn 
    210                 215                 220                 


Phe Gly Ala Gln His Ser Thr Thr Leu Phe Lys Ile Asp Asn Asn Thr 
225                 230                 235                 240 


Phe Val Arg Leu Asp Arg Pro His Thr Pro Gln Phe Leu Phe Gln Leu 
                245                 250                 255     


Asn Asp Thr Ile His Leu His Gln Gln Leu Ser Asn Thr Thr Gly Arg 
            260                 265                 270         


Leu Ile Trp Thr Leu Asp Ala Asn Ile Asn Ala Asp Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Asn Lys Lys Asn Leu Ser Glu Gln Leu Arg Gly Glu 
    290                 295                 300                 


Glu Leu Ser Phe Glu Ala Leu Ser Leu Asn Glu Thr Glu Asp Asp Asp 
305                 310                 315                 320 


Ala Ala Ser Ser Arg Ile Thr Lys Gly Arg Ile Ser Asp Arg Ala Thr 
                325                 330                 335     


Arg Lys Tyr Ser Asp Leu Val Pro Lys Asn Ser Pro Gly Met Val Pro 
            340                 345                 350         


Leu His Ile Pro Glu Gly Glu Thr Thr Leu Pro Ser Gln Asn Ser Thr 
        355                 360                 365             


Glu Gly Arg Arg Val Gly Val Asn Thr Gln Glu Thr Ile Thr Glu Thr 
    370                 375                 380                 


Ala Ala Thr Ile Ile Gly Thr Asn Gly Asn His Met Gln Ile Ser Thr 
385                 390                 395                 400 


Ile Gly Ile Arg Pro Ser Ser Ser Gln Ile Pro Ser Ser Ser Pro Thr 
                405                 410                 415     


Thr Ala Pro Ser Pro Glu Ala Gln Thr Pro Thr Thr His Thr Ser Gly 
            420                 425                 430         


Pro Ser Val Met Ala Thr Glu Glu Pro Thr Thr Pro Pro Gly Ser Ser 
        435                 440                 445             


Pro Gly Pro Thr Thr Glu Ala Pro Thr Leu Thr Thr Pro Glu Asn Ile 
    450                 455                 460                 


Thr Thr Ala Val Lys Thr Val Leu Pro Gln Glu Ser Thr Ser Asn Gly 
465                 470                 475                 480 


Leu Ile Thr Ser Thr Val Thr Gly Ile Leu Gly Ser Leu Gly Leu Arg 
                485                 490                 495     


Lys Arg Ser Arg Arg Gln Thr Asn Thr Lys Ala Thr Gly Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Ala Gln Glu Gln His Asn Ala Ala Gly 
        515                 520                 525             


Ile Ala Trp Ile Pro Tyr Phe Gly Pro Gly Ala Glu Gly Ile Tyr Thr 
    530                 535                 540                 


Glu Gly Leu Met His Asn Gln Asn Ala Leu Val Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Tyr Thr Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Arg Arg Trp Gly Gly Thr Cys Arg Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asn 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Ile Asp Asn Pro Leu Pro Asn Gln Asp Asn 
625                 630                 635                 640 


Asp Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly Ile 
                645                 650                 655     


Gly Ile Thr Gly Ile Ile Ile Ala Ile Ile Ala Leu Leu Cys Val Cys 
            660                 665                 670         


Lys Leu Leu Cys 
        675     


<210>  5
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike glycoprotein (Tai Forest ebolavirus)

<400>  5

Met Gly Ala Ser Gly Ile Leu Gln Leu Pro Arg Glu Arg Phe Arg Lys 
1               5                   10                  15      


Thr Ser Phe Phe Val Trp Val Ile Ile Leu Phe His Lys Val Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Val His Asn Asn Thr Leu Gln Val Ser Asp Ile 
        35                  40                  45              


Asp Lys Phe Val Cys Arg Asp Lys Leu Ser Ser Thr Ser Gln Leu Lys 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Thr Ala Thr Lys Arg Trp Gly Phe Arg Ala Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Cys Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Ala 
            100                 105                 110         


Ile Lys Lys Val Asp Gly Ser Glu Cys Leu Pro Glu Ala Pro Glu Gly 
        115                 120                 125             


Val Arg Asp Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Pro Gly Gly Leu Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Ile Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Ile Ala Phe Leu Ile Leu Pro Lys Ala Arg Lys Asp 
            180                 185                 190         


Phe Phe Gln Ser Pro Pro Leu His Glu Pro Ala Asn Met Thr Thr Asp 
        195                 200                 205             


Pro Ser Ser Tyr Tyr His Thr Thr Thr Ile Asn Tyr Val Val Asp Asn 
    210                 215                 220                 


Phe Gly Thr Asn Thr Thr Glu Phe Leu Phe Gln Val Asp His Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ala Arg Phe Thr Pro Gln Phe Leu Val Leu Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Ser Asp Asn Arg Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Ile Asn Pro Thr Val Asp Thr Ser Met Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Asn Lys Lys Asn Phe Thr Lys Thr Leu Ser Ser Glu 
    290                 295                 300                 


Glu Leu Ser Phe Val Pro Val Pro Glu Thr Gln Asn Gln Val Leu Asp 
305                 310                 315                 320 


Thr Thr Ala Thr Val Ser Pro Pro Ile Ser Ala His Asn His Ala Ala 
                325                 330                 335     


Glu Asp His Lys Glu Leu Val Ser Glu Asp Ser Thr Pro Val Val Gln 
            340                 345                 350         


Met Gln Asn Ile Lys Gly Lys Asp Thr Met Pro Thr Thr Val Thr Gly 
        355                 360                 365             


Val Pro Thr Thr Thr Pro Ser Pro Phe Pro Ile Asn Ala Arg Asn Thr 
    370                 375                 380                 


Asp His Thr Lys Ser Phe Ile Gly Leu Glu Gly Pro Gln Glu Asp His 
385                 390                 395                 400 


Ser Thr Thr Gln Pro Ala Lys Thr Thr Ser Gln Pro Thr Asn Ser Thr 
                405                 410                 415     


Glu Ser Thr Thr Leu Asn Pro Thr Ser Glu Pro Ser Ser Arg Gly Thr 
            420                 425                 430         


Gly Pro Ser Ser Pro Thr Val Pro Asn Thr Thr Glu Ser His Ala Glu 
        435                 440                 445             


Leu Gly Lys Thr Thr Pro Thr Thr Leu Pro Glu Gln His Thr Ala Ala 
    450                 455                 460                 


Ser Ala Ile Pro Arg Ala Val His Pro Asp Glu Leu Ser Gly Pro Gly 
465                 470                 475                 480 


Phe Leu Thr Asn Thr Ile Arg Gly Val Thr Asn Leu Leu Thr Gly Ser 
                485                 490                 495     


Arg Arg Lys Arg Arg Asp Val Thr Pro Asn Thr Gln Pro Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Ala Leu Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Thr 
    530                 535                 540                 


Glu Gly Ile Met Glu Asn Gln Asn Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro Gln Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Val Asp Asn Asn Leu Pro Asn Gln Asn Asp 
625                 630                 635                 640 


Gly Ser Asn Trp Trp Thr Gly Trp Lys Gln Trp Val Pro Ala Gly Ile 
                645                 650                 655     


Gly Ile Thr Gly Val Ile Ile Ala Ile Ile Ala Leu Leu Cys Ile Cys 
            660                 665                 670         


Lys Phe Met Leu 
        675     


<210>  6
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike glycoprotein (Bundibugyo ebolavirus)

<400>  6

Met Val Thr Ser Gly Ile Leu Gln Leu Pro Arg Glu Arg Phe Arg Lys 
1               5                   10                  15      


Thr Ser Phe Phe Val Trp Val Ile Ile Leu Phe His Lys Val Phe Pro 
            20                  25                  30          


Ile Pro Leu Gly Val Val His Asn Asn Thr Leu Gln Val Ser Asp Ile 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Ser Gln Leu Lys 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Thr Ala Thr Lys Arg Trp Gly Phe Arg Ala Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Asp 
            100                 105                 110         


Ile Lys Lys Ala Asp Gly Ser Glu Cys Leu Pro Glu Ala Pro Glu Gly 
        115                 120                 125             


Val Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Pro Glu Gly Tyr Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Ile Ile Tyr Arg Ser Thr Thr Phe 
                165                 170                 175     


Ser Glu Gly Val Val Ala Phe Leu Ile Leu Pro Glu Thr Lys Lys Asp 
            180                 185                 190         


Phe Phe Gln Ser Pro Pro Leu His Glu Pro Ala Asn Met Thr Thr Asp 
        195                 200                 205             


Pro Ser Ser Tyr Tyr His Thr Val Thr Leu Asn Tyr Val Ala Asp Asn 
    210                 215                 220                 


Phe Gly Thr Asn Met Thr Asn Phe Leu Phe Gln Val Asp His Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Pro Arg Phe Thr Pro Gln Phe Leu Val Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Asn Gly Arg Arg Ser Asn Thr Thr Gly Thr 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Thr Val Asp Thr Gly Val Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Asn Lys Lys Asn Phe Thr Lys Thr Leu Ser Ser Glu 
    290                 295                 300                 


Glu Leu Ser Val Ile Phe Val Pro Arg Ala Gln Asp Pro Gly Ser Asn 
305                 310                 315                 320 


Gln Lys Thr Lys Val Thr Pro Thr Ser Phe Ala Asn Asn Gln Thr Ser 
                325                 330                 335     


Lys Asn His Glu Asp Leu Val Pro Glu Asp Pro Ala Ser Val Val Gln 
            340                 345                 350         


Val Arg Asp Leu Gln Arg Glu Asn Thr Val Pro Thr Pro Pro Pro Asp 
        355                 360                 365             


Thr Val Pro Thr Thr Leu Ile Pro Asp Thr Met Glu Glu Gln Thr Thr 
    370                 375                 380                 


Ser His Tyr Glu Pro Pro Asn Ile Ser Arg Asn His Gln Glu Arg Asn 
385                 390                 395                 400 


Asn Thr Ala His Pro Glu Thr Leu Ala Asn Asn Pro Pro Asp Asn Thr 
                405                 410                 415     


Thr Pro Ser Thr Pro Pro Gln Asp Gly Glu Arg Thr Ser Ser His Thr 
            420                 425                 430         


Thr Pro Ser Pro Arg Pro Val Pro Thr Ser Thr Ile His Pro Thr Thr 
        435                 440                 445             


Arg Glu Thr His Ile Pro Thr Thr Met Thr Thr Ser His Asp Thr Asp 
    450                 455                 460                 


Ser Asn Arg Pro Asn Pro Ile Asp Ile Ser Glu Ser Thr Glu Pro Gly 
465                 470                 475                 480 


Pro Leu Thr Asn Thr Thr Arg Gly Ala Ala Asn Leu Leu Thr Gly Ser 
                485                 490                 495     


Arg Arg Thr Arg Arg Glu Ile Thr Leu Arg Thr Gln Ala Lys Cys Asn 
            500                 505                 510         


Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu Gly Ala Ala Ile Gly 
        515                 520                 525             


Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr Thr 
    530                 535                 540                 


Glu Gly Ile Met His Asn Gln Asn Gly Leu Ile Cys Gly Leu Arg Gln 
545                 550                 555                 560 


Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala Thr 
                565                 570                 575     


Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg Lys Ala Ile Asp Phe 
            580                 585                 590         


Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile Leu Gly Pro Asp Cys 
        595                 600                 605             


Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Lys Ile Asp 
    610                 615                 620                 


Gln Ile Ile His Asp Phe Ile Asp Lys Pro Leu Pro Asp Gln Thr Asp 
625                 630                 635                 640 


Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp Val Pro Ala Gly Ile 
                645                 650                 655     


Gly Ile Thr Gly Val Ile Ile Ala Val Ile Ala Leu Leu Cys Ile Cys 
            660                 665                 670         


Lys Phe Leu Leu 
        675     


<210>  7
<211>  677
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spike glycoprotein (Reston ebolavirus)

<400>  7

Met Gly Ser Gly Tyr Gln Leu Leu Gln Leu Pro Arg Glu Arg Phe Arg 
1               5                   10                  15      


Lys Thr Ser Phe Leu Val Trp Val Ile Ile Leu Phe Gln Arg Ala Ile 
            20                  25                  30          


Ser Met Pro Leu Gly Ile Val Thr Asn Ser Thr Leu Lys Ala Thr Glu 
        35                  40                  45              


Ile Asp Gln Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Ser Gln Leu 
    50                  55                  60                  


Lys Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Ile Ala Thr Asp Val 
65                  70                  75                  80  


Pro Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys 
                85                  90                  95      


Val Val Ser Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu 
            100                 105                 110         


Glu Ile Lys Lys Ser Asp Gly Ser Glu Cys Leu Pro Leu Pro Pro Asp 
        115                 120                 125             


Gly Val Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Gln Gly 
    130                 135                 140                 


Thr Gly Pro Cys Pro Gly Asp Leu Ala Phe His Lys Asn Gly Ala Phe 
145                 150                 155                 160 


Phe Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr 
                165                 170                 175     


Phe Ala Glu Gly Val Val Ala Phe Leu Ile Leu Ser Glu Pro Lys Lys 
            180                 185                 190         


His Phe Trp Lys Ala Thr Pro Ala His Glu Pro Val Asn Thr Thr Asp 
        195                 200                 205             


Asp Ser Thr Ser Tyr Tyr Met Thr Leu Thr Leu Ser Tyr Glu Met Ser 
    210                 215                 220                 


Asn Phe Gly Gly Asn Glu Ser Asn Thr Leu Phe Lys Val Asp Asn His 
225                 230                 235                 240 


Thr Tyr Val Gln Leu Asp Arg Pro His Thr Pro Gln Phe Leu Val Gln 
                245                 250                 255     


Leu Asn Glu Thr Leu Arg Arg Asn Asn Arg Leu Ser Asn Ser Thr Gly 
            260                 265                 270         


Arg Leu Thr Trp Thr Leu Asp Pro Lys Ile Glu Pro Asp Val Gly Glu 
        275                 280                 285             


Trp Ala Phe Trp Glu Thr Lys Lys Asn Phe Ser Gln Gln Leu His Gly 
    290                 295                 300                 


Glu Asn Leu His Phe Gln Ile Pro Ser Thr His Thr Asn Asn Ser Ser 
305                 310                 315                 320 


Asp Gln Ser Pro Ala Gly Thr Val Gln Gly Lys Ile Ser Tyr His Pro 
                325                 330                 335     


Pro Ala Asn Asn Ser Glu Leu Val Pro Thr Asp Ser Pro Pro Val Val 
            340                 345                 350         


Ser Val Leu Thr Ala Gly Arg Thr Glu Glu Met Ser Thr Gln Gly Leu 
        355                 360                 365             


Thr Asn Gly Glu Thr Ile Thr Gly Phe Thr Ala Asn Pro Met Thr Thr 
    370                 375                 380                 


Thr Ile Ala Pro Ser Pro Thr Met Thr Ser Glu Val Asp Asn Asn Val 
385                 390                 395                 400 


Pro Ser Glu Gln Pro Asn Asn Thr Ala Ser Ile Glu Asp Ser Pro Pro 
                405                 410                 415     


Ser Ala Ser Asn Glu Thr Ile Tyr His Ser Glu Met Asp Pro Ile Gln 
            420                 425                 430         


Gly Ser Asn Asn Ser Ala Gln Ser Pro Gln Thr Lys Thr Thr Pro Ala 
        435                 440                 445             


Pro Thr Thr Ser Pro Met Thr Gln Asp Pro Gln Glu Thr Ala Asn Ser 
    450                 455                 460                 


Ser Lys Pro Gly Thr Ser Pro Gly Ser Ala Ala Gly Pro Ser Gln Pro 
465                 470                 475                 480 


Gly Leu Thr Ile Asn Thr Val Ser Lys Val Ala Asp Ser Leu Ser Pro 
                485                 490                 495     


Thr Arg Lys Gln Lys Arg Ser Val Arg Gln Asn Thr Ala Asn Lys Cys 
            500                 505                 510         


Asn Pro Asp Leu Tyr Tyr Trp Thr Ala Val Asp Glu Gly Ala Ala Val 
        515                 520                 525             


Gly Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala Glu Gly Ile Tyr 
    530                 535                 540                 


Ile Glu Gly Val Met His Asn Gln Asn Gly Leu Ile Cys Gly Leu Arg 
545                 550                 555                 560 


Gln Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu Phe Leu Arg Ala 
                565                 570                 575     


Thr Thr Glu Leu Arg Thr Tyr Ser Leu Leu Asn Arg Lys Ala Ile Asp 
            580                 585                 590         


Phe Leu Leu Gln Arg Trp Gly Gly Thr Cys Arg Ile Leu Gly Pro Ser 
        595                 600                 605             


Cys Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile Thr Asp Glu Ile 
    610                 615                 620                 


Asn Gln Ile Lys His Asp Phe Ile Asp Asn Pro Leu Pro Asp His Gly 
625                 630                 635                 640 


Asp Asp Leu Asn Leu Trp Thr Gly Trp Arg Gln Trp Ile Pro Ala Gly 
                645                 650                 655     


Ile Gly Ile Ile Gly Val Ile Ile Ala Ile Ile Ala Leu Leu Cys Ile 
            660                 665                 670         


Cys Lys Ile Leu Cys 
        675         


<210>  8
<211>  1902
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EboGPdeltaM/V3

<400>  8
atgggtgtga ccggtatcct gcagctgccg cgtgatcgct tcaaacgtac ctctttcttt       60

ctgtgggtta tcatcctgtt ccagcgtacc ttttctatcc cgctgggtgt tattcataac      120

tccaccctgc aggtgagcga cgttgataaa ctggtttgcc gtgacaaact gtcttctacc      180

aaccagctgc gctccgtggg cctgaacctg gaaggtaacg gtgttgcaac cgacgtgccg      240

tctgcgacca aacgctgggg tttccgctcc ggtgttccgc cgaaagttgt taactacgaa      300

gcgggcgaat gggctgaaaa ctgttataac ctggaaatca agaaaccgga cggctccgag      360

tgcctgccgg cagctccgga cggtattcgc ggctttccgc gctgtcgtta cgttcataaa      420

gttagcggta ctggtccgtg cgcaggtgac tttgctttcc acaaagaggg cgcgtttttc      480

ctgtatgacc gcctggcatc caccgttatt taccgtggca ccaccttcgc ggaaggcgtt      540

gtggcgttcc tgatcctgcc gcaggctaag aaagatttct ttagcagcca cccgctgcgc      600

gagccggtta acgcgactga ggatccgtct tctggttatt actccaccac tatccgttac      660

caggcaactg gtttcggtac caacgaaact gaatacctgt tcgaagttga taacctgacc      720

tacgttcagc tggaaagccg cttcactccg cagttcctgc tgcagctgaa cgaaaccatc      780

tacaccagcg gtaaacgttc caacaccacc ggcaaactga tctggaaagt taacccggag      840

atcgatacca ctattggtga gtgggcgttt tgggaaacca agaaaaacct gacccgcaaa      900

atccgttccg aggaaggatt caatggaaca ggaccatgta caaatgtcag cacagtacaa      960

tgtacacatg gaatcaggcc agtagtatca actcaactgc tgttaaatgg cagtctagca     1020

gaagaagatg tagtaattag atctgccaat ttcacagaca atgctaaaac cataatagta     1080

cagctgaaca catctgtaga aattaattgt acaagaccca acaacaatac aagaaaaagt     1140

atccgtatcc agaggggacc agggagagca tttgttacaa taggaaaaat aggaaatatg     1200

agacaagcac attgtaacat tagtagagca aaatggaatg ccactttaaa acagatagct     1260

agcaaattaa gagaacaatt tggaaataat aaaacaataa tctttaagca atcctcagga     1320

aacaccatcg caggtgttgc tggtctgatc accggcggtc gtcgtacccg ccgtgaagct     1380

attgttaacg cacagccgaa atgtaacccg aacctgcact actggaccac tcaggatgaa     1440

ggcgctgcta tcggcctggc atggatcccg tacttcggtc cggcggctga aggtatctat     1500

atcgaaggtc tgatgcacaa ccaggatggt ctgatttgcg gtctgcgtca gctggcgaac     1560

gaaaccactc aggcgctgca gctgttcctg cgcgcaacca ccgagctgcg taccttctct     1620

atcctgaacc gtaaggcgat cgactttctg ctgcagcgtt ggggtggtac ctgccatatc     1680

ctgggtccgg actgctgtat cgagccgcat gattggacta aaaacatcac tgacaaaatc     1740

gaccagatca ttcacgactt cgttgacaaa accctgccgg accagggcga taacgacaac     1800

tggtggaccg gctggcgtca gtggattccg gcaggcatcg gcgttaccgg tgttattatt     1860

gctgtgattg cactgttttg catttgcaag ttcgttttct ga                        1902


<210>  9
<211>  633
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EboGPdeltaM/V3

<400>  9

Met Gly Val Thr Gly Ile Leu Gln Leu Pro Arg Asp Arg Phe Lys Arg 
1               5                   10                  15      


Thr Ser Phe Phe Leu Trp Val Ile Ile Leu Phe Gln Arg Thr Phe Ser 
            20                  25                  30          


Ile Pro Leu Gly Val Ile His Asn Ser Thr Leu Gln Val Ser Asp Val 
        35                  40                  45              


Asp Lys Leu Val Cys Arg Asp Lys Leu Ser Ser Thr Asn Gln Leu Arg 
    50                  55                  60                  


Ser Val Gly Leu Asn Leu Glu Gly Asn Gly Val Ala Thr Asp Val Pro 
65                  70                  75                  80  


Ser Ala Thr Lys Arg Trp Gly Phe Arg Ser Gly Val Pro Pro Lys Val 
                85                  90                  95      


Val Asn Tyr Glu Ala Gly Glu Trp Ala Glu Asn Cys Tyr Asn Leu Glu 
            100                 105                 110         


Ile Lys Lys Pro Asp Gly Ser Glu Cys Leu Pro Ala Ala Pro Asp Gly 
        115                 120                 125             


Ile Arg Gly Phe Pro Arg Cys Arg Tyr Val His Lys Val Ser Gly Thr 
    130                 135                 140                 


Gly Pro Cys Ala Gly Asp Phe Ala Phe His Lys Glu Gly Ala Phe Phe 
145                 150                 155                 160 


Leu Tyr Asp Arg Leu Ala Ser Thr Val Ile Tyr Arg Gly Thr Thr Phe 
                165                 170                 175     


Ala Glu Gly Val Val Ala Phe Leu Ile Leu Pro Gln Ala Lys Lys Asp 
            180                 185                 190         


Phe Phe Ser Ser His Pro Leu Arg Glu Pro Val Asn Ala Thr Glu Asp 
        195                 200                 205             


Pro Ser Ser Gly Tyr Tyr Ser Thr Thr Ile Arg Tyr Gln Ala Thr Gly 
    210                 215                 220                 


Phe Gly Thr Asn Glu Thr Glu Tyr Leu Phe Glu Val Asp Asn Leu Thr 
225                 230                 235                 240 


Tyr Val Gln Leu Glu Ser Arg Phe Thr Pro Gln Phe Leu Leu Gln Leu 
                245                 250                 255     


Asn Glu Thr Ile Tyr Thr Ser Gly Lys Arg Ser Asn Thr Thr Gly Lys 
            260                 265                 270         


Leu Ile Trp Lys Val Asn Pro Glu Ile Asp Thr Thr Ile Gly Glu Trp 
        275                 280                 285             


Ala Phe Trp Glu Thr Lys Lys Asn Leu Thr Arg Lys Ile Arg Ser Glu 
    290                 295                 300                 


Glu Gly Phe Asn Gly Thr Gly Pro Cys Thr Asn Val Ser Thr Val Gln 
305                 310                 315                 320 


Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                325                 330                 335     


Gly Ser Leu Ala Glu Glu Asp Val Val Ile Arg Ser Ala Asn Phe Thr 
            340                 345                 350         


Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Asn Thr Ser Val Glu Ile 
        355                 360                 365             


Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gln 
    370                 375                 380                 


Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 
385                 390                 395                 400 


Arg Gln Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 
                405                 410                 415     


Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly Asn Asn Lys Thr 
            420                 425                 430         


Ile Ile Phe Lys Gln Ser Ser Gly Asn Thr Ile Ala Gly Val Ala Gly 
        435                 440                 445             


Leu Ile Thr Gly Gly Arg Arg Thr Arg Arg Glu Ala Ile Val Asn Ala 
    450                 455                 460                 


Gln Pro Lys Cys Asn Pro Asn Leu His Tyr Trp Thr Thr Gln Asp Glu 
465                 470                 475                 480 


Gly Ala Ala Ile Gly Leu Ala Trp Ile Pro Tyr Phe Gly Pro Ala Ala 
                485                 490                 495     


Glu Gly Ile Tyr Ile Glu Gly Leu Met His Asn Gln Asp Gly Leu Ile 
            500                 505                 510         


Cys Gly Leu Arg Gln Leu Ala Asn Glu Thr Thr Gln Ala Leu Gln Leu 
        515                 520                 525             


Phe Leu Arg Ala Thr Thr Glu Leu Arg Thr Phe Ser Ile Leu Asn Arg 
    530                 535                 540                 


Lys Ala Ile Asp Phe Leu Leu Gln Arg Trp Gly Gly Thr Cys His Ile 
545                 550                 555                 560 


Leu Gly Pro Asp Cys Cys Ile Glu Pro His Asp Trp Thr Lys Asn Ile 
                565                 570                 575     


Thr Asp Lys Ile Asp Gln Ile Ile His Asp Phe Val Asp Lys Thr Leu 
            580                 585                 590         


Pro Asp Gln Gly Asp Asn Asp Asn Trp Trp Thr Gly Trp Arg Gln Trp 
        595                 600                 605             


Ile Pro Ala Gly Ile Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala 
    610                 615                 620                 


Leu Phe Cys Ile Cys Lys Phe Val Phe 
625                 630             


<210>  10
<211>  2565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIVenv of pNL4.3

<400>  10
atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg       60

ctccttggga tattgatgat ctgtagtgct acagaaaaat tgtgggtcac agtctattat      120

ggggtacctg tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca      180

tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg      300

gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaaccc cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc      420

aatagtagta gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat      480

atcagcacaa gcataagaga taaggtgcag aaagaatatg cattctttta taaacttgat      540

atagtaccaa tagataatac cagctatagg ttgataagtt gtaacacctc agtcattaca      600

caggcctgtc caaaggtatc ctttgagcca attcccatac attattgtgc cccggctggt      660

tttgcgattc taaaatgtaa taataagacg ttcaatggaa caggaccatg tacaaatgtc      720

agcacagtac aatgtacaca tggaatcagg ccagtagtat caactcaact gctgttaaat      780

ggcagtctag cagaagaaga tgtagtaatt agatctgcca atttcacaga caatgctaaa      840

accataatag tacagctgaa cacatctgta gaaattaatt gtacaagacc caacaacaat      900

acaagaaaaa gtatccgtat ccagagggga ccagggagag catttgttac aataggaaaa      960

ataggaaata tgagacaagc acattgtaac attagtagag caaaatggaa tgccacttta     1020

aaacagatag ctagcaaatt aagagaacaa tttggaaata ataaaacaat aatctttaag     1080

caatcctcag gaggggaccc agaaattgta acgcacagtt ttaattgtgg aggggaattt     1140

ttctactgta attcaacaca actgtttaat agtacttggt ttaatagtac ttggagtact     1200

gaagggtcaa ataacactga aggaagtgac acaatcacac tcccatgcag aataaaacaa     1260

tttataaaca tgtggcagga agtaggaaaa gcaatgtatg cccctcccat cagtggacaa     1320

attagatgtt catcaaatat tactgggctg ctattaacaa gagatggtgg taataacaac     1380

aatgggtccg agatcttcag acctggagga ggcgatatga gggacaattg gagaagtgaa     1440

ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag     1500

agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc     1560

ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga     1620

caattattgt ctgatatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa     1680

cagcatctgt tgcaactcac agtctggggc atcaaacagc tccaggcaag aatcctggct     1740

gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc     1800

atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt     1860

tggaataaca tgacctggat ggagtgggac agagaaatta acaattacac aagcttaata     1920

cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa     1980

ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata     2040

aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt     2100

tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca     2160

atcccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga     2220

gacagatcca ttcgattagt gaacggatcc ttagcactta tctgggacga tctgcggagc     2280

ctgtgcctct tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg     2340

gaacttctgg gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacagtat     2400

tggagtcagg aactaaagaa tagtgctgtt aacttgctca atgccacagc catagcagta     2460

gctgagggga cagatagggt tatagaagta ttacaagcag cttatagagc tattcgccac     2520

atacctagaa gaataagaca gggcttggaa aggattttgc tataa                     2565


<210>  11
<211>  854
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIVenv of pNL4.3

<400>  11

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Val Pro Ile Asp Asn Thr Ser Tyr Arg Leu Ile 
            180                 185                 190         


Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Ser Phe 
        195                 200                 205             


Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu 
    210                 215                 220                 


Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr Asn Val 
225                 230                 235                 240 


Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln 
                245                 250                 255     


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val Ile Arg Ser 
            260                 265                 270         


Ala Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Asn Thr 
        275                 280                 285             


Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 
    290                 295                 300                 


Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 
305                 310                 315                 320 


Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala Lys Trp 
                325                 330                 335     


Asn Ala Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly 
            340                 345                 350         


Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp Pro Glu 
        355                 360                 365             


Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
    370                 375                 380                 


Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr 
385                 390                 395                 400 


Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Gly Ser Glu 
    450                 455                 460                 


Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
    530                 535                 540                 


Asp Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln 
545                 550                 555                 560 


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp 
        595                 600                 605             


Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn Asn Met 
    610                 615                 620                 


Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile 
625                 630                 635                 640 


His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
                645                 650                 655     


Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe Asn 
            660                 665                 670         


Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile Val Gly 
        675                 680                 685             


Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile Val Asn 
    690                 695                 700                 


Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His Leu Pro 
705                 710                 715                 720 


Ile Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu Gly Gly 
                725                 730                 735     


Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser Leu Ala 
            740                 745                 750         


Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr His Arg 
        755                 760                 765             


Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu Leu Gly 
    770                 775                 780                 


Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu Gln Tyr 
785                 790                 795                 800 


Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Asn Leu Leu Asn Ala Thr 
                805                 810                 815     


Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val Leu Gln 
            820                 825                 830         


Ala Ala Tyr Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg Gln Gly 
        835                 840                 845             


Leu Glu Arg Ile Leu Leu 
    850                 


<210>  12
<211>  2133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIVenv/SIVtm

<400>  12
atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg       60

ctccttggga tattgatgat ctgtagtgct acagaaaaat tgtgggtcac agtctattat      120

ggggtacctg tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca      180

tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg      300

gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaaccc cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc      420

aatagtagta gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat      480

atcagcacaa gcataagaga taaggtgcag aaagaatatg cattctttta taaacttgat      540

atagtaccaa tagataatac cagctatagg ttgataagtt gtaacacctc agtcattaca      600

caggcctgtc caaaggtatc ctttgagcca attcccatac attattgtgc cccggctggt      660

tttgcgattc taaaatgtaa taataagacg ttcaatggaa caggaccatg tacaaatgtc      720

agcacagtac aatgtacaca tggaatcagg ccagtagtat caactcaact gctgttaaat      780

ggcagtctag cagaagaaga tgtagtaatt agatctgcca atttcacaga caatgctaaa      840

accataatag tacagctgaa cacatctgta gaaattaatt gtacaagacc caacaacaat      900

acaagaaaaa gtatccgtat ccagagggga cccgggagag catttgttac aataggaaaa      960

ataggaaata tgagacaagc acattgtaac attagtagag caaaatggaa tgccacttta     1020

aaacagatag ctagcaaatt aagagaacaa tttggaaata ataaaacaat aatctttaag     1080

caatcctcag gaggggaccc agaaattgta acgcacagtt ttaattgtgg aggggaattt     1140

ttctactgta attcaacaca actgtttaat agtacttggt ttaatagtac ttggagtact     1200

gaagggtcaa ataacactga aggaagtgac acaatcacac tcccatgcag aataaaacaa     1260

tttataaaca tgtggcagga agtaggaaaa gcaatgtatg cccctcccat cagtggacaa     1320

attagatgtt catcaaatat tactgggctg ctattaacaa gagatggtgg taataacaac     1380

aatgagtccg agatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa     1440

ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag     1500

agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc     1560

ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga     1620

caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa     1680

cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct     1740

gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc     1800

atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt     1860

tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata     1920

cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa     1980

ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata     2040

aagtacatcc agtacggagt ctacatcgtc gtaggagtca tcctgttgag gatcgtgatc     2100

tacatcgtcc agatgctggc taagttgctc tag                                  2133


<210>  13
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIVenv/SIVtm

<400>  13

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Val Pro Ile Asp Asn Thr Ser Tyr Arg Leu Ile 
            180                 185                 190         


Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Ser Phe 
        195                 200                 205             


Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu 
    210                 215                 220                 


Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr Asn Val 
225                 230                 235                 240 


Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln 
                245                 250                 255     


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val Ile Arg Ser 
            260                 265                 270         


Ala Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Asn Thr 
        275                 280                 285             


Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 
    290                 295                 300                 


Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 
305                 310                 315                 320 


Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala Lys Trp 
                325                 330                 335     


Asn Ala Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly 
            340                 345                 350         


Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp Pro Glu 
        355                 360                 365             


Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
    370                 375                 380                 


Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr 
385                 390                 395                 400 


Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Glu Ser Glu 
    450                 455                 460                 


Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
    530                 535                 540                 


Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln 
545                 550                 555                 560 


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp 
        595                 600                 605             


Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn His Thr 
    610                 615                 620                 


Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile 
625                 630                 635                 640 


His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
                645                 650                 655     


Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe Asn 
            660                 665                 670         


Ile Thr Asn Trp Leu Trp Tyr Ile Lys Tyr Ile Gln Tyr Gly Val Tyr 
        675                 680                 685             


Ile Val Val Gly Val Ile Leu Leu Arg Ile Val Ile Tyr Ile Val Gln 
    690                 695                 700                 


Met Leu Ala Lys Leu Leu 
705                 710 


<210>  14
<211>  2151
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIVenv/truncation713

<400>  14
atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg       60

ctccttggga tattgatgat ctgtagtgct acagaaaaat tgtgggtcac agtctattat      120

ggggtacctg tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca      180

tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg      300

gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaaccc cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc      420

aatagtagta gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat      480

atcagcacaa gcataagaga taaggtgcag aaagaatatg cattctttta taaacttgat      540

atagtaccaa tagataatac cagctatagg ttgataagtt gtaacacctc agtcattaca      600

caggcctgtc caaaggtatc ctttgagcca attcccatac attattgtgc cccggctggt      660

tttgcgattc taaaatgtaa taataagacg ttcaatggaa caggaccatg tacaaatgtc      720

agcacagtac aatgtacaca tggaatcagg ccagtagtat caactcaact gctgttaaat      780

ggcagtctag cagaagaaga tgtagtaatt agatctgcca atttcacaga caatgctaaa      840

accataatag tacagctgaa cacatctgta gaaattaatt gtacaagacc caacaacaat      900

acaagaaaaa gtatccgtat ccagagggga cccgggagag cattttatac aacaggagaa      960

ataataggag atataagaca agcacattgc aacattagta gaacaaaatg gaataacact     1020

ttaaatcaaa tagctacaaa attaaaagaa caatttggga ataataaaac aatagtcttt     1080

aatcaatcct caggagggga cccagaaatt gtaatgcaca gttttaattg tggaggggaa     1140

tttttctact gtaattcaac acaactgttt aatagtactt ggaattttaa tggtacttgg     1200

aatttaacac aatcgaatgg tactgaagga aatgacacta tcacactccc atgtagaata     1260

aaacaaatta taaatatgtg gcaggaagta ggaaaagcaa tgtatgcccc tcccatcaga     1320

ggacaaatta gatgctcatc aaatattaca gggctaatat taacaagaga tggtggaact     1380

aacagtagtg ggtccgagat cttcagacct gggggaggag atatgaggga caattggaga     1440

agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag     1500

gcaaaaagaa gagtggtgca gagagaaaaa agagcagtgg gaacgatagg agctatgttc     1560

cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaataac gctgacggta     1620

caggccagac tattattgtc tggtatagtg caacagcaga acaatttgct gagggctatt     1680

gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga     1740

gtcctggctg tggaaagata cctaagggat caacagctcc tagggatttg gggttgctct     1800

ggaaaactca tctgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg     1860

gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca     1920

agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa     1980

ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg     2040

tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt     2100

gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattcta g              2151


<210>  15
<211>  716
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIVenv/truncation713

<400>  15

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Val Pro Ile Asp Asn Thr Ser Tyr Arg Leu Ile 
            180                 185                 190         


Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Ser Phe 
        195                 200                 205             


Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu 
    210                 215                 220                 


Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr Asn Val 
225                 230                 235                 240 


Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln 
                245                 250                 255     


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val Ile Arg Ser 
            260                 265                 270         


Ala Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Asn Thr 
        275                 280                 285             


Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 
    290                 295                 300                 


Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Tyr Thr Thr Gly Glu 
305                 310                 315                 320 


Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Ile Ser Arg Thr Lys 
                325                 330                 335     


Trp Asn Asn Thr Leu Asn Gln Ile Ala Thr Lys Leu Lys Glu Gln Phe 
            340                 345                 350         


Gly Asn Asn Lys Thr Ile Val Phe Asn Gln Ser Ser Gly Gly Asp Pro 
        355                 360                 365             


Glu Ile Val Met His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys 
    370                 375                 380                 


Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Asn Phe Asn Gly Thr Trp 
385                 390                 395                 400 


Asn Leu Thr Gln Ser Asn Gly Thr Glu Gly Asn Asp Thr Ile Thr Leu 
                405                 410                 415     


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Lys 
            420                 425                 430         


Ala Met Tyr Ala Pro Pro Ile Arg Gly Gln Ile Arg Cys Ser Ser Asn 
        435                 440                 445             


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Thr Asn Ser Ser Gly 
    450                 455                 460                 


Ser Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
465                 470                 475                 480 


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
                485                 490                 495     


Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala 
            500                 505                 510         


Val Gly Thr Ile Gly Ala Met Phe Leu Gly Phe Leu Gly Ala Ala Gly 
        515                 520                 525             


Ser Thr Met Gly Ala Ala Ser Ile Thr Leu Thr Val Gln Ala Arg Leu 
    530                 535                 540                 


Leu Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile 
545                 550                 555                 560 


Glu Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln 
                565                 570                 575     


Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln 
            580                 585                 590         


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala 
        595                 600                 605             


Val Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp 
    610                 615                 620                 


Asn His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr 
625                 630                 635                 640 


Ser Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys 
                645                 650                 655     


Asn Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn 
            660                 665                 670         


Trp Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met 
        675                 680                 685             


Ile Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser 
    690                 695                 700                 


Ile Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Phe 
705                 710                 715     


<210>  16
<211>  134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence upstream of HIV envelop gene

<400>  16
gctagtctaa cttctagctt ctgaacaatc cccggtttac tcagtctccc ctaattccag       60

cctctcgaac aactaatatc ctgtcttttc tatccctatg aaaaaaacta acagagatcg      120

atctgtttac gcgt                                                        134


<210>  17
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence upstream of Ebola GP

<400>  17
cctaggaaag taactcaaat cctgctaggt atgaaaaaaa ctaacagata tcacgctcga       60

g                                                                       61


<210>  18
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence upstream of VSV-L

<400>  18
gctagccaga ttcttcatgt ttggaccaaa tcaacttgtg ataccatgct caaagaggcc       60

tcaattatat ttgagttttt aatttttatg aaaaaaacta acagcaatc                  109


<210>  19
<211>  12118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pATX-VSV genomic plasmid

<400>  19
acgcgactag taatacgact cactatagga cgaagacaaa caaaccatta ttatcattaa       60

aaggctcagg agaaacttta acagtaatca aaggtaccaa ttaattcccg ggcgcataat      120

atcagaacga agcaaatatt tattagctag gtatgaaaaa aactaacaga tatcagcgta      180

cgtaattata ggttaccacg aagacaaaca aaccattatt atcattaaaa ggctcaggag      240

aaactttaac agtaatcaaa atgtctgtta cagtcaagag aatcattgac aacacagtcg      300

tagttccaaa acttcctgca aatgaggatc cagtggaata cccggcagat tacttcagaa      360

aatcaaagga gattcctctt tacatcaata ctacaaaaag tttgtcagat ctaagaggat      420

atgtctacca aggcctcaaa tccggaaatg tatcaatcat acatgtcaac agctacttgt      480

atggagcatt aaaggacatc cggggtaagt tggataaaga ttggtcaagt ttcggaataa      540

acatcgggaa agcaggggat acaatcggaa tatttgacct tgtatccttg aaagccctgg      600

acggcgtact tccagatgga gtatcggatg cttccagaac cagcgcagat gacaaatggt      660

tgcctttgta tctacttggc ttatacagag tgggcagaac acaaatgcct gaatacagaa      720

aaaagctcat ggatgggctg acaaatcaat gcaaaatgat caatgaacag tttgaacctc      780

ttgtgccaga aggtcgtgac atttttgatg tgtggggaaa tgacagtaat tacacaaaaa      840

ttgtcgctgc agtggacatg ttcttccaca tgttcaaaaa acatgaatgt gcctcgttca      900

gatacggaac tattgtttcc agattcaaag attgtgctgc attggcaaca tttggacacc      960

tctgcaaaat aaccggaatg tctacagaag atgtaacgac ctggatcttg aaccgagaag     1020

ttgcagatga aatggtccaa atgatgcttc caggccaaga aattgacaag gccgattcat     1080

acatgcctta tttgatcgac tttggattgt cttctaagtc tccatattct tccgtcaaaa     1140

accctgcctt ccacttctgg gggcaattga cagctcttct gctcagatcc accagagcaa     1200

ggaatgcccg acagcctgat gacattgagt atacatctct tactacagca ggtttgttgt     1260

acgcttatgc agtaggatcc tctgccgact tggcacaaca gttttgtgtt ggagataaca     1320

aatacactcc agatgatagt accggaggat tgacgactaa tgcaccgcca caaggcagag     1380

atgtggtcga atggctcgga tggtttgaag atcaaaacag aaaaccgact cctgatatga     1440

tgcagtatgc gaaaagagca gtcatgtcac tgcaaggcct aagagagaag acaattggca     1500

agtatgctaa gtcagaattt gacaaatgac cctataattc tcagatcacc tattatatat     1560

tatgctacat atgaaaaaaa ctaacagata tcatggataa tctcacaaaa gttcgtgagt     1620

atctcaagtc ctattctcgt ctggatcagg cggtaggaga gatagatgag atcgaagcac     1680

aacgagctga aaagtccaat tatgagttgt tccaagagga tggagtggaa gagcatacta     1740

agccctctta ttttcaggca gcagatgatt ctgacacaga atctgaacca gaaattgaag     1800

acaatcaagg cttgtatgca ccagatccag aagctgagca agttgaaggc tttatacagg     1860

ggcctttaga tgactatgca gatgaggaag tggatgttgt atttacttcg gactggaaac     1920

agcctgagct tgaatctgac gagcatggaa agaccttacg gttgacatcg ccagagggtt     1980

taagtggaga gcagaaatcc cagtggcttt cgacgattaa agcagtcgtg caaagtgcca     2040

aatactggaa tctggcagag tgcacatttg aagcatcggg agaaggggtc attatgaagg     2100

agcgccagat aactccggat gtatataagg tcactccagt gatgaacaca catccgtccc     2160

aatcagaagc agtatcagat gtttggtctc tctcaaagac atccatgact ttccaaccca     2220

agaaagcaag tcttcagcct ctcaccatat ccttggatga attgttctca tctagaggag     2280

agttcatctc tgtcggaggt gacggacgaa tgtctcataa agaggccatc ctgctcggcc     2340

tgagatacaa aaagttgtac aatcaggcga gagtcaaata ttctctgtag actatgaaaa     2400

aaagtaacag atatcacgat ctaagtgtta tcccaatcca ttcatcatga gttccttaaa     2460

gaagattctc ggtctgaagg ggaaaggtaa gaaatctaag aaattaggga tcgcaccacc     2520

cccttatgaa gaggacacta gcatggagta tgctccgagc gctccaattg acaaatccta     2580

ttttggagtt gacgagatgg acacctatga tccgaatcaa ttaagatatg agaaattctt     2640

ctttacagtg aaaatgacgg ttagatctaa tcgtccgttc agaacatact cagatgtggc     2700

agccgctgta tcccattggg atcacatgta catcggaatg gcagggaaac gtcccttcta     2760

caaaatcttg gcttttttgg gttcttctaa tctaaaggcc actccagcgg tattggcaga     2820

tcaaggtcaa ccagagtatc acgctcactg cgaaggcagg gcttatttgc cacataggat     2880

ggggaagacc cctcccatgc tcaatgtacc agagcacttc agaagaccat tcaatatagg     2940

tctttacaag ggaacgattg agctcacaat gaccatctac gatgatgagt cactggaagc     3000

agctcctatg atctgggatc atttcaattc ttccaaattt tctgatttca gagagaaggc     3060

cttaatgttt ggcctgattg tcgagaaaaa ggcatctgga gcgtgggtcc tggactctat     3120

cggccacttc aaatgagcta gtctaacttc tagcttctga acaatccccg gtttactcag     3180

tctcccctaa ttccagcctc tcgaacaact aatatcctgt cttttctatc cctatgaaaa     3240

aaactaacag agatcgatct gtttacgcgt cactatcaag tgccttttgt acttagcctt     3300

tttatgcatg cggtacgaca tagagatgaa ccgcctagga aagtaactca aatcctgcta     3360

ggtatgaaaa aaactaacag atatcacgct cgagaattaa ttgctaggta tgaaaaaaac     3420

taacagatat cacgctcgag aattaattgc tagccagatt cttcatgttt ggaccaaatc     3480

aacttgtgat accatgctca aagaggcctc aattatattt gagtttttaa tttttatgaa     3540

aaaaactaac agcaatcatg gaagtccacg attttgagac cgacgagttc aatgatttca     3600

atgaagatga ctatgccaca agagaattcc tgaatcccga tgagcgcatg acgtacttga     3660

atcatgctga ttacaacctg aattctcctc taattagtga tgatattgac aatttaatca     3720

ggaaattcaa ttctcttcca attccctcga tgtgggatag taagaactgg gatggagttc     3780

ttgagatgtt aacgtcatgt caagccaatc ccatcccaac atctcagatg cataaatgga     3840

tgggaagttg gttaatgtct gataatcatg atgccagtca agggtatagt tttttacatg     3900

aagtggacaa agaggcagaa ataacatttg acgtggtgga gaccttcatc cgcggctggg     3960

gcaacaaacc aattgaatac atcaaaaagg aaagatggac tgactcattc aaaattctcg     4020

cttatttgtg tcaaaagttt ttggacttac acaagttgac attaatctta aatgctgtct     4080

ctgaggtgga attgctcaac ttggcgagga ctttcaaagg caaagtcaga agaagttctc     4140

atggaacgaa catatgcagg attagggttc ccagcttggg tcctactttt atttcagaag     4200

gatgggctta cttcaagaaa cttgatattc taatggaccg aaactttctg ttaatggtca     4260

aagatgtgat tatagggagg atgcaaacgg tgctatccat ggtatgtaga atagacaacc     4320

tgttctcaga gcaagacatc ttctcccttc taaatatcta cagaattgga gataaaattg     4380

tggagaggca gggaaatttt tcttatgact tgattaaaat ggtggaaccg atatgcaact     4440

tgaagctgat gaaattagca agagaatcaa ggcctttagt cccacaattc cctcattttg     4500

aaaatcatat caagacttct gttgatgaag gggcaaaaat tgaccgaggt ataagattcc     4560

tccatgatca gataatgagt gtgaaaacag tggatctcac actggtgatt tatggatcgt     4620

tcagacattg gggtcatcct tttatagatt attacactgg actagaaaaa ttacattccc     4680

aagtaaccat gaagaaagat attgatgtgt catatgcaaa agcacttgca agtgatttag     4740

ctcggattgt tctatttcaa cagttcaatg atcataaaaa gtggttcgtg aatggagact     4800

tgctccctca tgatcatccc tttaaaagtc atgttaaaga aaatacatgg cccacagctg     4860

ctcaagttca agattttgga gataaatggc atgaacttcc gctgattaaa tgttttgaaa     4920

tacccgactt actagaccca tcgataatat actctgacaa aagtcattca atgaataggt     4980

cagaggtgtt gaaacatgtc cgaatgaatc cgaacactcc tatccctagt aaaaaggtgt     5040

tgcagactat gttggacaca aaggctacca attggaaaga atttcttaaa gagattgatg     5100

agaagggctt agatgatgat gatctaatta ttggtcttaa aggaaaggag agggaactga     5160

agttggcagg tagatttttc tccctaatgt cttggaaatt gcgagaatac tttgtaatta     5220

ccgaatattt gataaagact catttcgtcc ctatgtttaa aggcctgaca atggcggacg     5280

atctaactgc agtcattaaa aagatgttag attcctcatc cggccaagga ttgaagtcat     5340

atgaggcaat ttgcatagcc aatcacattg attacgaaaa atggaataac caccaaagga     5400

agttatcaaa cggcccagtg ttccgagtta tgggccagtt cttaggttat ccatccttaa     5460

tcgagagaac tcatgaattt tttgagaaaa gtcttatata ctacaatgga agaccagact     5520

tgatgcgtgt tcacaacaac acactgatca attcaacctc ccaacgagtt tgttggcaag     5580

gacaagaggg tggactggaa ggtctacggc aaaaaggatg gagtatcctc aatctactgg     5640

ttattcaaag agaggctaaa atcagaaaca ctgctgtcaa agtcttggca caaggtgata     5700

atcaagttat ttgcacacag tataaaacga agaaatcgag aaacgttgta gaattacagg     5760

gtgctctcaa tcaaatggtt tctaataatg agaaaattat gactgcaatc aaaataggga     5820

cagggaagtt aggacttttg ataaatgacg atgagactat gcaatctgca gattacttga     5880

attatggaaa aataccgatt ttccgtggag tgattagagg gttagagacc aagagatggt     5940

cacgagtgac ttgtgtcacc aatgaccaaa tacccacttg tgctaatata atgagctcag     6000

tttccacaaa tgctctcacc gtagctcatt ttgctgagaa cccaatcaat gccatgatac     6060

agtacaatta ttttgggaca tttgctagac tcttgttgat gatgcatgat cctgctcttc     6120

gtcaatcatt gtatgaagtt caagataaga taccgggctt gcacagttct actttcaaat     6180

acgccatgtt gtatttggac ccttccattg gaggagtgtc gggcatgtct ttgtccaggt     6240

ttttgattag agccttccca gatcccgtaa cagaaagtct ctcattctgg agattcatcc     6300

atgtacatgc tcgaagtgag catctgaagg agatgagtgc agtatttgga aaccccgaga     6360

tagccaagtt tcgaataact cacatagaca agctagtaga agatccaacc tctctgaaca     6420

tcgctatggg aatgagtcca gcgaacttgt taaagactga ggttaaaaaa tgcttaatcg     6480

aatcaagaca aaccatcagg aaccaggtga ttaaggatgc aaccatatat ttgtatcatg     6540

aagaggatcg gctcagaagt ttcttatggt caataaatcc tctgttccct agatttttaa     6600

gtgaattcaa atcaggcact tttttgggag tcgcagacgg gctcatcagt ctatttcaaa     6660

attctcgtac tattcggaac tcctttaaga aaaagtatca tagggaattg gatgatttga     6720

ttgtgaggag tgaggtatcc tctttgacac atttagggaa acttcatttg agaaggggat     6780

catgtaaaat gtggacatgt tcagctactc atgctgacac attaagatac aaatcctggg     6840

gccgtacagt tattgggaca actgtacccc atccattaga aatgttgggt ccacaacatc     6900

gaaaagagac tccttgtgca ccatgtaaca catcagggtt caattatgtt tctgtgcatt     6960

gtccagacgg gatccatgac gtctttagtt cacggggacc attgcctgct tatctagggt     7020

ctaaaacatc tgaatctaca tctattttgc agccttggga aagggaaagc aaagtcccac     7080

tgattaaaag agctacacgt cttagagatg ctatctcttg gtttgttgaa cccgactcta     7140

aactagcaat gactatactt tctaacatcc actctttaac aggcgaagaa tggaccaaaa     7200

ggcagcatgg gttcaaaaga acagggtctg cccttcatag gttttcgaca tctcggatga     7260

gccatggtgg gttcgcatct cagagcactg cagcattgac caggttgatg gcaactacag     7320

acaccatgag ggatctggga gatcagaatt tcgacttttt attccaagca acgttgctct     7380

atgctcaaat taccaccact gttgcaagag acggatggat caccagttgt acagatcatt     7440

atcatattgc ctgtaagtcc tgtttgagac ccatagaaga gatcaccctg gactcaagta     7500

tggactacac gcccccagat gtatcccatg tgctgaagac atggaggaat ggggaaggtt     7560

cgtggggaca agagataaaa cagatctatc ctttagaagg gaattggaag aatttagcac     7620

ctgctgagca atcctatcaa gtcggcagat gtataggttt tctatatgga gacttggcgt     7680

atagaaaatc tactcatgcc gaggacagtt ctctatttcc tctatctata caaggtcgta     7740

ttagaggtcg aggtttctta aaagggttgc tagacggatt aatgagagca agttgctgcc     7800

aagtaataca ccggagaagt ctggctcatt tgaagaggcc ggccaacgca gtgtacggag     7860

gtttgattta cttgattgat aaattgagtg tatcacctcc attcctttct cttactagat     7920

caggacctat tagagacgaa ttagaaacga ttccccacaa gatcccaacc tcctatccga     7980

caagcaaccg tgatatgggg gtgattgtca gaaattactt caaataccaa tgccgtctaa     8040

ttgaaaaggg aaaatacaga tcacattatt cacaattatg gttattctca gatgtcttat     8100

ccatagactt cattggacca ttctctattt ccaccaccct cttgcaaatc ctatacaagc     8160

catttttatc tgggaaagat aagaatgagt tgagagagct ggcaaatctt tcttcattgc     8220

taagatcagg agaggggtgg gaagacatac atgtgaaatt cttcaccaag gacatattat     8280

tgtgtccaga ggaaatcaga catgcttgca agttcgggat tgctaaggat aataataaag     8340

acatgagcta tcccccttgg ggaagggaat ccagagggac aattacaaca atccctgttt     8400

attatacgac caccccttac ccaaagatgc tagagatgcc tccaagaatc caaaatcccc     8460

tgctgtccgg aatcaggttg ggccaattac caactggcgc tcattataaa attcggagta     8520

tattacatgg aatgggaatc cattacaggg acttcttgag ttgtggagac ggctccggag     8580

ggatgactgc tgcattacta cgagaaaatg tgcatagcag aggaatattc aatagtctgt     8640

tagaattatc agggtcagtc atgcgaggcg cctctcctga gccccccagt gccctagaaa     8700

ctttaggagg agataaatcg agatgtgtaa atggtgaaac atgttgggaa tatccatctg     8760

acttatgtga cccaaggact tgggactatt tcctccgact caaagcaggc ttggggcttc     8820

aaattgattt aattgtaatg gatatggaag ttcgggattc ttctactagc ctgaaaattg     8880

agacgaatgt tagaaattat gtgcaccgga ttttggatga gcaaggagtt ttaatctaca     8940

agacttatgg aacatatatt tgtgagagcg aaaagaatgc agtaacaatc cttggtccca     9000

tgttcaagac ggtcgactta gttcaaacag aatttagtag ttctcaaacg tctgaagtat     9060

atatggtatg taaaggtttg aagaaattaa tcgatgaacc caatcccgat tggtcttcca     9120

tcaatgaatc ctggaaaaac ctgtacgcat tccagtcatc agaacaggaa tttgccagag     9180

caaagaaggt tagtacatac tttaccttga caggtattcc ctcccaattc attcctgatc     9240

cttttgtaaa cattgagact atgctacaaa tattcggagt acccacgggt gtgtctcatg     9300

cggctgcctt aaaatcatct gatagacctg cagatttatt gaccattagc cttttttata     9360

tggcgattat atcgtattat aacatcaatc atatcagagt aggaccgata cctccgaacc     9420

ccccatcaga tggaattgca caaaatgtgg ggatcgctat aactggtata agcttttggc     9480

tgagtttgat ggagaaagac attccactat atcaacagtg tttagcagtt atccagcaat     9540

cattcccgat taggtgggag gctgtttcag taaaaggagg atacaagcag aagtggagta     9600

ctagaggtga tgggctccca aaagataccc gaatttcaga ctccttggcc ccaatcggga     9660

actggatcag atctctggaa ttggtccgaa accaagttcg tctaaatcca ttcaatgaga     9720

tcttgttcaa tcagctatgt cgtacagtgg ataatcattt gaaatggtca aatttgcgaa     9780

gaaacacagg aatgattgaa tggatcaata gacgaatttc aaaagaagac cggtctatac     9840

tgatgttgaa gagtgaccta cacgaggaaa actcttggag agattaaaaa atcatgagga     9900

gactccaaac tttaagtatg aaaaaaactt tgatccttaa gaccctcttg tggtttttat     9960

tttttatctg gttttgtggt cttcgtgggt cggcatggca tctccacctc ctcgcggtcc    10020

gacctgggca tccgaaggag gacgtcgtcc actcggatgg ctaagggagg ggcccccgcg    10080

ggggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa    10140

ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaagggagg    10200

aactatatcc ggatcgagac ctcgatacta gagcggccgc caccgcggtg gagctcaaag    10260

gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa    10320

ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc    10380

cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca    10440

ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg    10500

accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct    10560

catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt    10620

gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag    10680

tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc    10740

agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac    10800

actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga    10860

gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc    10920

aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg    10980

gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca    11040

aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt    11100

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca    11160

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg    11220

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca    11280

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt    11340

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt    11400

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca    11460

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca    11520

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga    11580

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact    11640

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga    11700

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg    11760

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc    11820

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga    11880

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat    11940

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt    12000

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt    12060

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg      12118


<210>  20
<211>  2127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIVenv/EboTM

<400>  20
atgagagtga aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg       60

ctccttggga tattgatgat ctgtagtgct acagaaaaat tgtgggtcac agtctattat      120

ggggtacctg tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca      180

tatgatacag aggtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac      240

ccacaagaag tagtattggt aaatgtgaca gaaaatttta acatgtggaa aaatgacatg      300

gtagaacaga tgcatgagga tataatcagt ttatgggatc aaagcctaaa gccatgtgta      360

aaattaaccc cactctgtgt tagtttaaag tgcactgatt tgaagaatga tactaatacc      420

aatagtagta gcgggagaat gataatggag aaaggagaga taaaaaactg ctctttcaat      480

atcagcacaa gcataagaga taaggtgcag aaagaatatg cattctttta taaacttgat      540

atagtaccaa tagataatac cagctatagg ttgataagtt gtaacacctc agtcattaca      600

caggcctgtc caaaggtatc ctttgagcca attcccatac attattgtgc cccggctggt      660

tttgcgattc taaaatgtaa taataagacg ttcaatggaa caggaccatg tacaaatgtc      720

agcacagtac aatgtacaca tggaatcagg ccagtagtat caactcaact gctgttaaat      780

ggcagtctag cagaagaaga tgtagtaatt agatctgcca atttcacaga caatgctaaa      840

accataatag tacagctgaa cacatctgta gaaattaatt gtacaagacc caacaacaat      900

acaagaaaaa gtatccgtat ccagagggga cccgggagag catttgttac aataggaaaa      960

ataggaaata tgagacaagc acattgtaac attagtagag caaaatggaa tgccacttta     1020

aaacagatag ctagcaaatt aagagaacaa tttggaaata ataaaacaat aatctttaag     1080

caatcctcag gaggggaccc agaaattgta acgcacagtt ttaattgtgg aggggaattt     1140

ttctactgta attcaacaca actgtttaat agtacttggt ttaatagtac ttggagtact     1200

gaagggtcaa ataacactga aggaagtgac acaatcacac tcccatgcag aataaaacaa     1260

tttataaaca tgtggcagga agtaggaaaa gcaatgtatg cccctcccat cagtggacaa     1320

attagatgtt catcaaatat tactgggctg ctattaacaa gagatggtgg taataacaac     1380

aatgggtccg agatcttcag acctggagga ggcgatatga gggacaattg gagaagtgaa     1440

ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag     1500

agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc     1560

ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga     1620

caattattgt ctgatatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa     1680

cagcatctgt tgcaactcac agtctggggc atcaaacagc tccaggcaag aatcctggct     1740

gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc     1800

atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt     1860

tggaataaca tgacctggat ggagtgggac agagaaatta acaattacac aagcttaata     1920

cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa     1980

ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata     2040

aagcaatgga taccggcagg tatcggagtc acaggcgtta tcatcgcagt tatcgctttg     2100

ttctgtatct gcaagttcgt cttctag                                         2127


<210>  21
<211>  708
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HIVenv/EboTM

<400>  21

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Lys 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Ile Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Asp Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Val Pro Ile Asp Asn Thr Ser Tyr Arg Leu Ile 
            180                 185                 190         


Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val Ser Phe 
        195                 200                 205             


Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu 
    210                 215                 220                 


Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr Asn Val 
225                 230                 235                 240 


Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser Thr Gln 
                245                 250                 255     


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Asp Val Val Ile Arg Ser 
            260                 265                 270         


Ala Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu Asn Thr 
        275                 280                 285             


Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser 
    290                 295                 300                 


Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys 
305                 310                 315                 320 


Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala Lys Trp 
                325                 330                 335     


Asn Ala Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln Phe Gly 
            340                 345                 350         


Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp Pro Glu 
        355                 360                 365             


Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn 
    370                 375                 380                 


Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr 
385                 390                 395                 400 


Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Phe Ile Asn Met Trp Gln Glu Val Gly Lys Ala Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Gly Ser Glu 
    450                 455                 460                 


Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
    530                 535                 540                 


Asp Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu Ala Gln 
545                 550                 555                 560 


Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val Pro Trp 
        595                 600                 605             


Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn Asn Met 
    610                 615                 620                 


Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser Leu Ile 
625                 630                 635                 640 


His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
                645                 650                 655     


Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp Phe Asn 
            660                 665                 670         


Ile Thr Asn Trp Leu Trp Tyr Ile Lys Gln Trp Ile Pro Ala Gly Ile 
        675                 680                 685             


Gly Val Thr Gly Val Ile Ile Ala Val Ile Ala Leu Phe Cys Ile Cys 
    690                 695                 700                 


Lys Phe Val Phe 
705             


<210>  22
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer GP-Makona-B6-F

<400>  22
agatatcacg ctcgagatgg gtgttacagg aatattgc                               38


<210>  23
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer GP-Makona-B6-R

<400>  23
gaagaatctg gctagcctaa aagacaaatt tgcatataca g                           41


<210>  24
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HIV/Trunc713-F

<400>  24
cgatctgttt acgcgtatga gagtgaagga gaagtatcag c                           41


<210>  25
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HIV/Trunc713-R

<400>  25
gagttacttt cctaggctag aatggtgaat atccctgcc                              39


<210>  26
<211>  130
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HIV/SIVtm-R

<400>  26
gagttacttt cctaggctag agcaacttag ccagcatctg gacgatgtag atcacgatcc       60

tcaacaggat gactcctacg acgatgtaga ctccgtactg gatgtacttt atataccaca      120

gccaatttgt                                                             130


<210>  27
<211>  134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer HIV/EboTM-R

<400>  27
aggatttgag ttactttcct aggctagaag acgaacttgc agatacagaa caaagcgata       60

actgcgatga taacgcctgt gactccgata cctgccggta tccattgttg ctttatatac      120

cacagccaat ttgt                                                        134


<210>  28
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-N-F

<400>  28
cggtatcgat aagcttatgt ctgttacagt caagag                                 36


<210>  29
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-N-R

<400>  29
cgggctgcag gaattctcat ttgtcaaatt ctgactta                               38


<210>  30
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-P-F

<400>  30
cggtatcgat aagcttatgg ataatctcac aaaagt                                 36


<210>  31
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-P-R

<400>  31
cgggctgcag gaattct                                                      17


<210>  32
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-L-F

<400>  32
ggtatcgata agcttatgga agtccacgat tttga                                  35


<210>  33
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pBS-L-R

<400>  33
cgggctgcag gaattcttaa tctctccaag agttttc                                37


<210>  34
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pCAGGs-T7-F

<400>  34
ttttggcaaa gaattcatga acacgattaa catcgctaag aacg                        44


<210>  35
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer pCAGGs-T7-R

<400>  35
agagggaaaa agatctttac gcgaacgcga agtcc                                  35


