                         SEQUENCE LISTING

<110>  Genovie AB
 
<120>  A cell surface tag exchange (CSTE) system for tracing and 
       manipulation of cells during recombinase mediated cassette 
       exchange integration of nucleic acid sequences to engineered 
       receiver cells

<130>  P020611PCT1

<150>  EP Appl No 18182353.5
<151>  2018-07-09

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  5837
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Tag-exchange receiver site encoding Myc CST and RFP selection 
       gene

<400>  1
gagctctact ggcttctgcg ccgcctctgg cccactgttt ccccttccca ggcaggtcct       60

gctttctctg acctgcattc tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg      120

gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc aggttccgtc      180

ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga tgctctttcc      240

ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt      300

ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct tcactcgctg      360

ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta ggatggcctt      420

ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc atcatcaccg      480

tttttctgga caaccccaaa gtaccccgtc tccctggctt tagccacctc tccatcctct      540

tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct cactcctttc      600

atttgggcag ctcccctacc ccccttacct ctctagtctg tgctagctct tccagccccc      660

tgtcatggca tcttccaggg gtccgagagc tcagctagtc ttcttcctcc aacccgggcc      720

cctatgtcca cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt ccccgagctg      780

ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta cttttatctg      840

tcccctccac cgggtggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc      900

cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg      960

gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa     1020

ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga     1080

acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct     1140

tgcgtgcctt gaattacttc cactggctgc agtacgtgat tcttgatccc gagcttcggg     1200

ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt     1260

gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg     1320

cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga     1380

cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt     1440

cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga     1500

ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc     1560

ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg     1620

cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga     1680

gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga     1740

aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt     1800

ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg     1860

ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt     1920

ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc     1980

tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgactgctta     2040

agcttggtac cgccaccatg gccaagggct tctacatcag caagagcctg ggcatcctgg     2100

gaatcctgct gggagtggct gctgtgtgca ccatcatcgc tctgagcgtg gtgtacagcc     2160

aggagggagg tcctggatcc ggaacaggtg gctctggcac tggaggatca ggtccaggtg     2220

gatctatggt gaagcagatc gagagcaaga ctgctttcca ggaagccttg gacgctgcag     2280

gtgataagct tgtagtagtt gacttctcag caacgtggtc tggaccttcc aagatgatca     2340

agcctttctt ccattccctc tctgagaagt attccaacgt gatattcctt gaagtagatg     2400

tggatgactc tcaggatgtt gcttcagagt gtgaagtcaa atccatgcca acattccagt     2460

tcttcaagaa gggacagaag gtgggtgaat tttctggagc caataaggag aagcttgaag     2520

ccaccattaa cgagttggta ggtaagtggc tggggccaga gactggctct tgaagttcct     2580

attccgaagt tcctattctc tagaaagtat aggaacttcc tggctcagaa aaagggccct     2640

gacaactctt ttcatcttct aggcggttcc tctacatccg gtggatctgg atctggagaa     2700

caaaagctca tctctgagga ggaccttggg gagcagaagc taatcagtga agaagacctc     2760

ggagagcaga aattgattag cgaggaggat ctttaaagac gctagcacct gatcctgatc     2820

ataatcaagc catatcacat ctgtagaggt ttacttgctt taaaaaacct ccacacctcc     2880

ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt     2940

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     3000

tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgct     3060

gaagagcgct gatcagctct tcaccataga gcccaccgca tccccagcat gcctgctatt     3120

gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca     3180

cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc     3240

accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga     3300

aggcacagtc gggtgctagc tcatctgtgg cccagcttgc taggcaggtc gcagtacttg     3360

gccacggcca tctcgtgctg ttccacgtag gtttctttgt cggcctcttt gatccgttcc     3420

agccggtggt ccacgaagtg gaagccgggc atcttcaggt tcttggcggg cttcttgctt     3480

ctgtaggtgg tcttgaagga gcagtgcagg tagcctccgc ccacgagttt cagggccatc     3540

tgagaatggc ctctcaggcc gccatcggca gggtacagca tctcggtgtt ggcctcccag     3600

cctctggttt tcttctgcat cacggggccg ttgctgggga agttcacgcc gttaatcttc     3660

acgttgtaga tgatgcagcc gttctggaag ctggtatcct gggtggcggt cagcactccg     3720

ccgtcctcgt atgtggtgat ccgctcccag gtgaagccct cggggaagct ctgcttgaag     3780

aaatcgggga tgccctgggt gtggttgatg aaggccttgc tgccgtacat aaagctggtg     3840

gccaggatat caaaggcgaa gggcagaggg ccgccttcca ccaccttgat cttcatggtc     3900

tgggtgccct cgtaaggctt gccctcgccc tcgctggtgc acttgaagtg gtggttgttc     3960

acggtgcctt ccatgtacag cttcatgtgc atgttttctt tgatcagctc gctcatggtg     4020

gctacggaag ttcctattcc gaagttccta ttcttcaaat agtataggaa cttccggtac     4080

cagtcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat     4140

gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc     4200

tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc     4260

cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg     4320

cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct     4380

ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc     4440

tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag     4500

ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg     4560

gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc     4620

cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat     4680

accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc     4740

agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg     4800

aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc     4860

acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg     4920

aagctcggga tcaagaatca cgtactgcag ccagtggaag taattcaagg cacgcaaggg     4980

ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg     5040

gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct     5100

cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg     5160

cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact     5220

gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caccggatca ggatcaggat     5280

tggtgacaga aaagccccat ccttaggcct cctccttcct agtctcctga tattgggtct     5340

aacccccacc tcctgttagg cagattcctt atctggtgac acacccccat ttcctggagc     5400

catctctctc cttgccagaa cctctaaggt ttgcttacga tggagccaga gaggatcctg     5460

ggagggagag cttggcaggg ggtgggaggg aaggggggga tgcgtgacct gcccggttct     5520

cagtggccac cctgcgctac cctctcccag aacctgagct gctctgacgc ggctgtctgg     5580

tgcgtttcac tgatcctggt gctgcagctt ccttacactt cccaagagga gaagcagttt     5640

ggaaaaacaa aatcagaata agttggtcct gagttctaac tttggctctt cacctttcta     5700

gtccccaatt tatattgttc ctccgtgcgt cagttttacc tgtgagataa ggccagtagc     5760

cagccccgtc ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt     5820

gtgagaatgg tgcgtcc                                                    5837


<210>  2
<211>  1332
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Flippase

<400>  2
atggccccca agaaaaagcg gaaagtgggc atccacggcg tgccagctgc aggcggctct       60

atgagccagt tcgacatcct gtgcaagacc ccacctaagg tgctcgtgcg gcagttcgtg      120

gaaagattcg agaggcctag cggcgagaag atcgcctctt gtgctgccga gctgacctac      180

ctgtgctgga tgatcaccca caacggcacc gccatcaagc gggccacctt catgagctac      240

aataccatca tcagcaacag cctgagcttc gacatcgtga acaagagcct gcagttcaag      300

tacaagaccc agaaggccac catcctggaa gccagcctga agaaactgat ccccgcctgg      360

gagtttacca tcatcccata caatggccag aaacatcaga gcgacattac cgatatcgtg      420

tccagcctcc agctgcagtt cgagagtagc gaagaagccg acaagggcaa cagccacagc      480

aagaagatgc tgaaggccct gctgagcgag ggcgagagca tctgggagat cacagagaag      540

atcctgaaca gcttcgagta caccagccgg ttcaccaaga ccaagaccct gtaccagttc      600

ctgttcctgg ccacctttat caactgcggc cggttctccg acatcaagaa cgtggacccc      660

aagagcttca agctggtgca gaacaagtac ctgggcgtga tcattcagtg cctcgtgacc      720

gagacaaaga ccagcgtgtc ccggcacatc tactttttca gcgccagagg ccggatcgac      780

cccctggtgt acctggacga gttcctgaga aacagcgagc ccgtgctgaa gagagtgaac      840

cggaccggca acagcagctc caacaagcag gaataccagc tgctgaagga caacctcgtg      900

cggtcctaca acaaggccct gaagaaaaac gccccctacc ccatcttcgc cattaagaac      960

ggccccaagt cccacatcgg ccggcacctg atgaccagct ttctgagcat gaagggcctg     1020

acagagctga ccaacgtcgt gggcaattgg agcgacaaga gggcctctgc cgtggccaga     1080

accacctaca cccaccagat cacagccatc cccgaccact acttcgccct ggtgtctcgg     1140

tactacgcct acgaccccat cagcaaagag atgatcgccc tgaaggacga gacaaacccc     1200

atcgaggaat ggcagcacat cgagcagctg aagggcagcg ccgagggcag catcagatac     1260

cctgcctgga acggcatcat ctcccaggaa gtgctggact acctgagcag ctacatcaac     1320

cggcggatct ga                                                         1332


<210>  3
<211>  1791
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TEDV encoding SBP CST and P-gene_RSV1 GOI

<400>  3
gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct ggctcagaaa       60

aagggccctg acaactcttt tcatcttcta ggcggttcct ctacatccgg tggatctgga      120

tctgggcatg tggttgaggg gcttgctggc gaactagagc aattgcgagc ccgcctcgaa      180

caccatcctc aaggtcaata aagactcgag cacctgatcc tgatcataat caagccatat      240

cacatctgta gaggtttact tgctttaaaa aacctccaca cctccccctg aacctgaaac      300

ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat      360

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg      420

gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgctgaaga gcgctgatca      480

gctcttcacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc      540

ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg      600

cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa      660

ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgggtg      720

ctagcgaaga ccgagcggtg gatccgtatg gtgacaagac gactagtgtg aacgactaga      780

aagagaaccc ggcacatctg tcgagaggta cgaccgtgca ctaagcttcg caaggtcagt      840

ggagctatcg agtgtaggat ggttcaagtc tgccaatgga gctacagaag tcgaagccgt      900

ccagatctgg actatagtca cttatcgtca tcgtccttat aatcaatatc gtggtcttta      960

taatctccat catgatcctt gtaatccccg gggaaatctt ccaggctcag gtcgttgtcg     1020

ctgtcgttgc cttccagcag gttgttcagc ttctcagagg tagggttcag agacacttcg     1080

tcggaggtgt ccttggccat cttctcggat tcctcgtttc tcagccgggc catagcttcc     1140

agccgatcgt tggtcatcag ggcctctgtc cggatcttct cgatcatctc ttcccgcagt     1200

ccgaccatgg catctctgat gccatctctg gcagatgtag gtccggcaga tgccaccacc     1260

agtgtgtgca gcatgcccag gatctcagac agcttctcgt cgatccggtc cagtctggcg     1320

gtgatgttgt cgttggtctg gtcgttgatt tcctcgtagc tgtagctgct ctcttcctcg     1380

ttgttgtcga atgtctcgat tgtctctttg tacagcttgc tgaaggggtt gtcgctaggt     1440

gtggggtcct ctttgaagga caccaggggc tttctctggt agttgggctt gttgccggcg     1500

gtgtcgtctg tctcgttggt ggggttgatg atggtgctgt tgctggtgat ggggctctct     1560

ttggtcactt cgatgtcgat gctgttcacg ctgatgatgc tgtccttctt cttggggtcc     1620

ttggggctcg tgaacttgcc cttgatggat tccagaaact tggtggcccg attgttggcg     1680

tcctcgccgt ggaactcagg ggcgaacttt tccatggtgg cggtattgtc ttcggtaccg     1740

cacgaagttc ctattcggaa gttcctattc ttcaaatagt ataggaactt c              1791


<210>  4
<211>  2241
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TEDV encoding SBP CST and N-gene_RSV1 GOI

<400>  4
gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct ggctcagaaa       60

aagggccctg acaactcttt tcatcttcta ggcggttcct ctacatccgg tggatctgga      120

tctgggcatg tggttgaggg gcttgctggc gaactagagc aattgcgagc ccgcctcgaa      180

caccatcctc aaggtcaata aagactcgag cacctgatcc tgatcataat caagccatat      240

cacatctgta gaggtttact tgctttaaaa aacctccaca cctccccctg aacctgaaac      300

ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat      360

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg      420

gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgctgaaga gcgctgatca      480

gctcttcacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc      540

ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg      600

cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa      660

ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgggtg      720

ctagcgaaga ccgagcggtg gatccgtatg gtgacaagac gactagtgtg aacgactagg      780

gagagaaccc ggcacatctg tcgagaggta cgaccgtgca ctaagcttcg caaggtcagt      840

ggagctatcg agtgtaggat ggttcaagtc tgccaatgga gctacagaag tcgaagccgt      900

ccagatctgg actatagtca cttatcgtca tcgtccttat aatcaatatc gtggtcttta      960

taatctccat catgatcctt gtaatccccg ggcagttcca cgtcgttgtc cttagggttc     1020

agctggtgct tgatggcttc cagttcctcg gcggtcagat ccagcacgct gtagttgatc     1080

acgccgtttt ctttcagctg ctcggcatag gccttggcgg catcgtacag gtcctggttt     1140

ctaggggtgc ctctgtactc gcccatgatt cccagtccgg cggcatttcc cagcaccaca     1200

gagctgaagt gtgggaactg tgtcaggctc agcagagagg ccttggggtt gttcaggatg     1260

tggtaaaagc cggcctctcc gccaagcttc tgggcgtact cgtacacttc caccacctgt     1320

tccatttcgg cctgcacaga ggcgtggccc agcatgatgt tcttcacgct cttggccagc     1380

actccccatc tcagcatcac ttgtccggcg ccataggcat tcatgaacag gccggcgaag     1440

atgccttcca cccgagagcc gcctcttgtg ctgctctggg cgattccgaa gtgcacgaac     1500

acgtcgatga agtgagggta cttctcgaac acctcgtaga agctgttggc aatatcctta     1560

ggcagcaggc ccttgtaccg cttcatctcg ttcttcagca cgttgttggc tctccgaatc     1620

acagcggtca ggccagaccg atcgccagcg gccagtttgg tgatgaccag agcggcgata     1680

cacaggatga tcatgccgca atcagggctg tcgtgtctgt actcaggggc gacttcgccc     1740

atctctttca gcattttctt gtaggacttc cggctctcga tctcgatgtt gatctggatc     1800

tctgtggtca ggctggccag ggtcagcacc tcgaacttca tttctttgcc gttgatgtcc     1860

tgccggtgtg tggtcacgtc cacgccattg gccttcacgt ggtagccggc atctctcagg     1920

atcttgatgg tgtcctctct gcccagtctg ctcatggcgt acagcattcc gatcacgcct     1980

gtgaacttgt ggttggcgtc ctcggtgatc agcagcatgc cgcacagctt gttgatgtgc     2040

ttctgcacgt cgtagttagg ggtgtcgatg ctgtcgccgg tgcttctctg gatggtgtac     2100

ttgctgctgc tcagcagctg gtccttgttc agggtgtcgt tcagcttcac tttgctcagg     2160

gccatggtgg cggtattgtc ttcggtaccg cacgaagttc ctattcggaa gttcctattc     2220

ttcaaatagt ataggaactt c                                               2241


<210>  5
<211>  1649
<212>  DNA
<213>  Artificial sequence

<220>
<223>  M2-long-gene_RSV1

<400>  5
gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct ggctcagaaa       60

aagggccctg acaactcttt tcatcttcta ggcggttcct ctacatccgg tggatctgga      120

tctgggcatg tggttgaggg gcttgctggc gaactagagc aattgcgagc ccgcctcgaa      180

caccatcctc aaggtcaata aagactcgag cacctgatcc tgatcataat caagccatat      240

cacatctgta gaggtttact tgctttaaaa aacctccaca cctccccctg aacctgaaac      300

ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat      360

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg      420

gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgctgaaga gcgctgatca      480

gctcttcacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc      540

ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg      600

cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa      660

ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgggtg      720

ctagcgaaga ccgagcggtg gatccgtatg gtgacaagac gactagtgtg aacgacctgt      780

tagagaaccc ggcacatctg tcgagaggta cgaccgtgca ctaagcttcg caaggtcagt      840

ggagctatcg agtgtaggat ggttcaagtc tgccaatgga gctacagaag tcgaagccgt      900

ccagatctgg actatagtca cttatcgtca tcgtccttat aatcaatatc gtggtcttta      960

taatctccat catgatcctt gtaatccccg ggggtggtat cgttgttctt ggcgtggtcg     1020

ttggtgtcgt tgacggtgct ctctttggga ttgttgatgg tgatgctctt gtggatgtcc     1080

agggtgttct tgatggtttt cttcagcacg tcggcaggca gccgcttcag cagatggatg     1140

gtctgcttgt tgttcttccg gttgctctcg atgtagctga tcacggtgtt gtacacccgg     1200

atcttggggc tgttaggttc ctcgttgtcc cgcagcttct tgatgtcgtc gctgttcagc     1260

tctgtcagca gcttgctcat ggccacgcag gcgctctgct tggtgatgtt gttgatgctg     1320

ccgatatagc tttccagcac gcccacaacg cccagggcat attcctcggt tctatccagt     1380

tcggcggctc cgctgatctc gctcagggtg tcgatgctct tatccatgga cttcaggatt     1440

ctgttcagca tgaagttctg ccgcacgagc agagcgtgag gaggccactc gaagtagttg     1500

tggctgaagt ggcatctctt gccgttcagg cagtggcctc tgatctcgaa cttgcagggg     1560

ttccgtctgc tcatggtggc ggtattgtct tcggtaccgc acgaagttcc tattcggaag     1620

ttcctattct tcaaatagta taggaactt                                       1649


<210>  6
<211>  4542
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CMVpro-Flp-sv40pA-V2 vector V4.I.8

<400>  6
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggccgcatg aattcgctac cggtatagta atcaattacg gggtcattag ttcatagccc      420

atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa      480

cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac      540

tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca      600

agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg      660

gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt      720

agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg      780

gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg      840

gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat      900

gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca      960

gatcaggtac catggccccc aagaaaaagc ggaaagtggg catccacggc gtgccagctg     1020

caggcggctc tatgagccag ttcgacatcc tgtgcaagac cccacctaag gtgctcgtgc     1080

ggcagttcgt ggaaagattc gagaggccta gcggcgagaa gatcgcctct tgtgctgccg     1140

agctgaccta cctgtgctgg atgatcaccc acaacggcac cgccatcaag cgggccacct     1200

tcatgagcta caataccatc atcagcaaca gcctgagctt cgacatcgtg aacaagagcc     1260

tgcagttcaa gtacaagacc cagaaggcca ccatcctgga agccagcctg aagaaactga     1320

tccccgcctg ggagtttacc atcatcccat acaatggcca gaaacatcag agcgacatta     1380

ccgatatcgt gtccagcctc cagctgcagt tcgagagtag cgaagaagcc gacaagggca     1440

acagccacag caagaagatg ctgaaggccc tgctgagcga gggcgagagc atctgggaga     1500

tcacagagaa gatcctgaac agcttcgagt acaccagccg gttcaccaag accaagaccc     1560

tgtaccagtt cctgttcctg gccaccttta tcaactgcgg ccggttctcc gacatcaaga     1620

acgtggaccc caagagcttc aagctggtgc agaacaagta cctgggcgtg atcattcagt     1680

gcctcgtgac cgagacaaag accagcgtgt cccggcacat ctactttttc agcgccagag     1740

gccggatcga ccccctggtg tacctggacg agttcctgag aaacagcgag cccgtgctga     1800

agagagtgaa ccggaccggc aacagcagct ccaacaagca ggaataccag ctgctgaagg     1860

acaacctcgt gcggtcctac aacaaggccc tgaagaaaaa cgccccctac cccatcttcg     1920

ccattaagaa cggccccaag tcccacatcg gccggcacct gatgaccagc tttctgagca     1980

tgaagggcct gacagagctg accaacgtcg tgggcaattg gagcgacaag agggcctctg     2040

ccgtggccag aaccacctac acccaccaga tcacagccat ccccgaccac tacttcgccc     2100

tggtgtctcg gtactacgcc tacgacccca tcagcaaaga gatgatcgcc ctgaaggacg     2160

agacaaaccc catcgaggaa tggcagcaca tcgagcagct gaagggcagc gccgagggca     2220

gcatcagata ccctgcctgg aacggcatca tctcccagga agtgctggac tacctgagca     2280

gctacatcaa ccggcggatc tgatctagac ctgatcataa tcaagccata tcacatctgt     2340

agaggtttac ttgctttaaa aaacctccac acctccccct gaacctgaaa cataaaatga     2400

atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata     2460

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     2520

aactcatcaa tgtatcttat catgtctgga tctgcggatc caatctcgag ctgggcctca     2580

tgggccttcc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa     2640

catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg     2700

ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg     2760

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     2820

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     2880

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     2940

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     3000

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     3060

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa     3120

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg     3180

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag     3240

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt     3300

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     3360

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc     3420

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca     3480

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa     3540

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat     3600

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct     3660

taccatctgg ccccagtgct gcaatgatac cgcgagaacc acgctcaccg gctccagatt     3720

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat     3780

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta     3840

atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg     3900

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt     3960

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg     4020

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg     4080

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc     4140

ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa     4200

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac     4260

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt     4320

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg     4380

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa     4440

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata     4500

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac                        4542


<210>  7
<211>  10428
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SpCas9-2A-GFP vector V1.A.8

<400>  7
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgccaccat ggactataag gaccacgacg gagactacaa      960

ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga agaagcggaa     1020

ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc tggacatcgg     1080

caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt     1140

caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct     1200

gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata     1260

caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa     1320

ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa     1380

gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta     1440

ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg     1500

gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg     1560

cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta     1620

caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct     1680

gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga     1740

gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt     1800

caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga     1860

cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc     1920

cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat     1980

caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct     2040

gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt     2100

cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt     2160

ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa     2220

gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca     2280

ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt     2340

cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt     2400

gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac     2460

catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat     2520

cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag     2580

cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga     2640

gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct     2700

gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat     2760

cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg     2820

cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa     2880

cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat     2940

cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa     3000

gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga     3060

caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa     3120

cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca     3180

ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc     3240

cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg     3300

ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa     3360

gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg     3420

cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta     3480

cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg     3540

gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat     3600

cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc     3660

cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat     3720

tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga     3780

taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc     3840

acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga     3900

agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt     3960

ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt     4020

cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga     4080

ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc     4140

taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct     4200

ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat     4260

cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt     4320

gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc     4380

caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg     4440

cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg     4500

caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag     4560

cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa     4620

ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag     4680

aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata     4740

tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa     4800

tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca     4860

gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc     4920

cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct     4980

gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga     5040

ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat     5100

caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca aaaggccggc     5160

ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggaa ttcggcagtg gagagggcag     5220

aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtga gcaagggcga     5280

ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca     5340

caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa     5400

gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac     5460

ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa     5520

gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa     5580

ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct     5640

gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta     5700

caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt     5760

caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa     5820

cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc     5880

cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac     5940

cgccgccggg atcactctcg gcatggacga gctgtacaag gaattctaac gctagagggc     6000

ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt     6060

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     6120

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     6180

tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg     6240

tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg     6300

cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta     6360

cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt     6420

tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg     6480

ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat     6540

cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac     6600

tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag     6660

ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg     6720

cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc     6780

aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc     6840

aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt     6900

cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc     6960

ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct     7020

attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg     7080

agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat     7140

tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta     7200

tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca     7260

ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga     7320

cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga     7380

cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct     7440

cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg     7500

gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga     7560

gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca     7620

tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga     7680

ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg     7740

cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc     7800

gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt     7860

gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga     7920

gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca     7980

tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc     8040

cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac     8100

cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc     8160

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta     8220

tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag     8280

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     8340

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     8400

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     8460

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     8520

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     8580

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     8640

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     8700

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     8760

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     8820

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     8880

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     8940

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     9000

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     9060

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     9120

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     9180

tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg     9240

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     9300

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     9360

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     9420

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     9480

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     9540

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     9600

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     9660

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     9720

agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt     9780

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     9840

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     9900

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     9960

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg    10020

cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact    10080

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg    10140

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt    10200

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga    10260

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc    10320

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa    10380

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc                 10428


<210>  8
<211>  6071
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pcDNA3.1_GFP vector V1.A.4

<400>  8
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgccaccat ggaatccgat gagtctggcc tgcccgccat      960

ggaaatcgag tgcagaatca ccggcaccct gaacggcgtg gaatttgagc tcgtgggcgg     1020

aggcgagggc acacctgaac agggcagaat gaccaacaag atgaagtcca ccaagggggc     1080

cctgaccttc agcccctacc tgctgtctca cgtgatgggc tacggcttct accacttcgg     1140

cacctacccc agcggctacg agaacccttt cctgcacgcc atcaacaacg gcggctacac     1200

caacacccgg atcgagaagt acgaggacgg cggcgtgctg cacgtgtcct tcagctacag     1260

atacgaggcc ggcagagtga tcggcgactt caaagtgatg ggcaccggat tccccgagga     1320

cagcgtgatc ttcaccgaca agatcatccg gtccaacgcc accgtggaac atctgcaccc     1380

catgggcgac aacgacctgg acggcagctt caccagaacc ttctccctgc gggatggcgg     1440

ctactacagc agcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat     1500

cctccagaac ggcggaccca tgttcgcctt cagacgggtg gaagaggacc acagcaacac     1560

cgagctgggc atcgtggaat accagcacgc cttcaagacc cccgatgccg atgccggcga     1620

ggaatgagtc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc     1680

tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc     1740

cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg     1800

tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa     1860

tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg     1920

gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt     1980

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt     2040

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct     2100

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg     2160

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga     2220

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc     2280

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga     2340

gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt     2400

ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca     2460

gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat     2520

ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg     2580

cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc     2640

gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta     2700

ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca     2760

ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct     2820

tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc     2880

gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc     2940

ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc     3000

gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg     3060

ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc     3120

atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac     3180

caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat     3240

caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc     3300

aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg     3360

aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg     3420

gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc     3480

gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc     3540

gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg     3600

accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa     3660

ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc     3720

tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat     3780

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg     3840

gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga     3900

gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc     3960

cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct     4020

aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc     4080

agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt     4140

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     4200

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     4260

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     4320

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     4380

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     4440

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     4500

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     4560

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     4620

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     4680

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     4740

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     4800

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt     4860

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct     4920

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga     4980

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa     5040

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac     5100

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga     5160

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc     5220

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca     5280

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta     5340

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg     5400

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc     5460

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg     5520

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt     5580

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt     5640

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata     5700

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc     5760

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac     5820

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa     5880

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct     5940

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat     6000

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc     6060

cacctgacgt c                                                          6071


<210>  9
<211>  2762
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAVSI_sg-sp-opti_3 vector V2.J.6

<400>  9
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt      180

gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt      240

gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg      300

acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca      360

aggccgcatg gatccaaggt cgggcaggaa gagggcctat ttcccatgat tccttcatat      420

ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca      480

aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt      540

ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat      600

ttcttggctt tatatatctt gtggaaagga cgaaacaccg tcaccaatcc tgtccctagg      660

tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact      720

tgaaaaagtg gcaccgagtc ggtgcttttt ttcagacatc catagatcta gctcgagttt      780

tttttctaga ctgggcctca tgggccttcc gctcactgcc cgctttccag tcgggaaacc      840

tgtcgtgcca gctgcattaa catggtcata gctgtttcct tgcgtattgg gcgctctccg      900

cttcctcgct cactgactcg ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg      960

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     1020

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     1080

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     1140

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     1200

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     1260

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     1320

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     1380

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     1440

cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     1500

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     1560

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     1620

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     1680

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     1740

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     1800

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     1860

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaacc     1920

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     1980

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     2040

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     2100

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     2160

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     2220

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     2280

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     2340

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     2400

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     2460

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     2520

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     2580

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     2640

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     2700

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     2760

ac                                                                    2762


<210>  10
<211>  8188
<212>  DNA
<213>  Artificial sequence

<400>  10
gagctctact ggcttctgcg ccgcctctgg cccactgttt ccccttccca ggcaggtcct       60

gctttctctg acctgcattc tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg      120

gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc aggttccgtc      180

ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga tgctctttcc      240

ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt      300

ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct tcactcgctg      360

ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta ggatggcctt      420

ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc atcatcaccg      480

tttttctgga caaccccaaa gtaccccgtc tccctggctt tagccacctc tccatcctct      540

tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct cactcctttc      600

atttgggcag ctcccctacc ccccttacct ctctagtctg tgctagctct tccagccccc      660

tgtcatggca tcttccaggg gtccgagagc tcagctagtc ttcttcctcc aacccgggcc      720

cctatgtcca cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt ccccgagctg      780

ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta cttttatctg      840

tcccctccac cgggtggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc      900

cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg      960

gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa     1020

ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga     1080

acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct     1140

tgcgtgcctt gaattacttc cactggctgc agtacgtgat tcttgatccc gagcttcggg     1200

ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt     1260

gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg     1320

cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga     1380

cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt     1440

cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga     1500

ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc     1560

ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg     1620

cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga     1680

gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga     1740

aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt     1800

ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg     1860

ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt     1920

ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc     1980

tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgactgctta     2040

agcttggtac cgccaccatg gccaagggct tctacatcag caagagcctg ggcatcctgg     2100

gaatcctgct gggagtggct gctgtgtgca ccatcatcgc tctgagcgtg gtgtacagcc     2160

aggagggagg tcctggatcc ggaacaggtg gctctggcac tggaggatca ggtccaggtg     2220

gatctatggt gaagcagatc gagagcaaga ctgctttcca ggaagccttg gacgctgcag     2280

gtgataagct tgtagtagtt gacttctcag caacgtggtc tggaccttcc aagatgatca     2340

agcctttctt ccattccctc tctgagaagt attccaacgt gatattcctt gaagtagatg     2400

tggatgactc tcaggatgtt gcttcagagt gtgaagtcaa atccatgcca acattccagt     2460

tcttcaagaa gggacagaag gtgggtgaat tttctggagc caataaggag aagcttgaag     2520

ccaccattaa cgagttggta ggtaagtggc tggggccaga gactggctct tgaagttcct     2580

attccgaagt tcctattctc tagaaagtat aggaacttcc tggctcagaa aaagggccct     2640

gacaactctt ttcatcttct aggcggttcc tctacatccg gtggatctgg atctggagaa     2700

caaaagctca tctctgagga ggaccttggg gagcagaagc taatcagtga agaagacctc     2760

ggagagcaga aattgattag cgaggaggat ctttaaagac gctagcacct gatcctgatc     2820

ataatcaagc catatcacat ctgtagaggt ttacttgctt taaaaaacct ccacacctcc     2880

ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt     2940

ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac     3000

tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgct     3060

gaagagcgct gatcagctct tcaccataga gcccaccgca tccccagcat gcctgctatt     3120

gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca     3180

cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc     3240

accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga     3300

aggcacagtc gggtgctagc tcatctgtgg cccagcttgc taggcaggtc gcagtacttg     3360

gccacggcca tctcgtgctg ttccacgtag gtttctttgt cggcctcttt gatccgttcc     3420

agccggtggt ccacgaagtg gaagccgggc atcttcaggt tcttggcggg cttcttgctt     3480

ctgtaggtgg tcttgaagga gcagtgcagg tagcctccgc ccacgagttt cagggccatc     3540

tgagaatggc ctctcaggcc gccatcggca gggtacagca tctcggtgtt ggcctcccag     3600

cctctggttt tcttctgcat cacggggccg ttgctgggga agttcacgcc gttaatcttc     3660

acgttgtaga tgatgcagcc gttctggaag ctggtatcct gggtggcggt cagcactccg     3720

ccgtcctcgt atgtggtgat ccgctcccag gtgaagccct cggggaagct ctgcttgaag     3780

aaatcgggga tgccctgggt gtggttgatg aaggccttgc tgccgtacat aaagctggtg     3840

gccaggatat caaaggcgaa gggcagaggg ccgccttcca ccaccttgat cttcatggtc     3900

tgggtgccct cgtaaggctt gccctcgccc tcgctggtgc acttgaagtg gtggttgttc     3960

acggtgcctt ccatgtacag cttcatgtgc atgttttctt tgatcagctc gctcatggtg     4020

gctacggaag ttcctattcc gaagttccta ttcttcaaat agtataggaa cttccggtac     4080

cagtcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat     4140

gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc     4200

tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc     4260

cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg     4320

cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct     4380

ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc     4440

tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag     4500

ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg     4560

gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc     4620

cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat     4680

accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc     4740

agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg     4800

aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc     4860

acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg     4920

aagctcggga tcaagaatca cgtactgcag ccagtggaag taattcaagg cacgcaaggg     4980

ccataacccg taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg     5040

gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct     5100

cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg     5160

cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact     5220

gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc caccggatca ggatcaggat     5280

tggtgacaga aaagccccat ccttaggcct cctccttcct agtctcctga tattgggtct     5340

aacccccacc tcctgttagg cagattcctt atctggtgac acacccccat ttcctggagc     5400

catctctctc cttgccagaa cctctaaggt ttgcttacga tggagccaga gaggatcctg     5460

ggagggagag cttggcaggg ggtgggaggg aaggggggga tgcgtgacct gcccggttct     5520

cagtggccac cctgcgctac cctctcccag aacctgagct gctctgacgc ggctgtctgg     5580

tgcgtttcac tgatcctggt gctgcagctt ccttacactt cccaagagga gaagcagttt     5640

ggaaaaacaa aatcagaata agttggtcct gagttctaac tttggctctt cacctttcta     5700

gtccccaatt tatattgttc ctccgtgcgt cagttttacc tgtgagataa ggccagtagc     5760

cagccccgtc ctggcagggc tgtggtgagg aggggggtgt ccgtgtggaa aactcccttt     5820

gtgagaatgg tgcgtcctcg agctgggcct catgggcctt ccgctcactg cccgctttcc     5880

agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt     5940

gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg     6000

gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     6060

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     6120

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     6180

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     6240

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     6300

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     6360

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     6420

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     6480

gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc     6540

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     6600

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     6660

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     6720

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag     6780

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat     6840

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc     6900

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat     6960

accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag     7020

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg     7080

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc     7140

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca     7200

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg     7260

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc     7320

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta     7380

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc     7440

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg     7500

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc     7560

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc     7620

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat     7680

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag     7740

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc     7800

ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt     7860

tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca     7920

aaagaataga ccgagatagg gttgagtggc cgctacaggg cgctcccatt cgccattcag     7980

gctgcgcaac tgttgggaag ggcgtttcgg tgcgggcctc ttcgctatta cgccagctgg     8040

cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac     8100

gacgttgtaa aacgacggcc agtgagcgcg acgtaatacg actcactata gggcgaattg     8160

gcggaaggcc gtcaaggccg catgaatt                                        8188


<210>  11
<211>  3996
<212>  DNA
<213>  Artificial sequence

<220>
<223>  M2-gene-Long_RSV1-FLAG_TxV12.A.8

<400>  11
gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct ggctcagaaa       60

aagggccctg acaactcttt tcatcttcta ggcggttcct ctacatccgg tggatctgga      120

tctgggcatg tggttgaggg gcttgctggc gaactagagc aattgcgagc ccgcctcgaa      180

caccatcctc aaggtcaata aagactcgag cacctgatcc tgatcataat caagccatat      240

cacatctgta gaggtttact tgctttaaaa aacctccaca cctccccctg aacctgaaac      300

ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat      360

aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg      420

gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgctgaaga gcgctgatca      480

gctcttcacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc      540

ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg      600

cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa      660

ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgggtg      720

ctagcgaaga ccgagcggtg gatccgtatg gtgacaagac gactagtgtg aacgacctgt      780

tagagaaccc ggcacatctg tcgagaggta cgaccgtgca ctaagcttcg caaggtcagt      840

ggagctatcg agtgtaggat ggttcaagtc tgccaatgga gctacagaag tcgaagccgt      900

ccagatctgg actatagtca cttatcgtca tcgtccttat aatcaatatc gtggtcttta      960

taatctccat catgatcctt gtaatccccg ggggtggtat cgttgttctt ggcgtggtcg     1020

ttggtgtcgt tgacggtgct ctctttggga ttgttgatgg tgatgctctt gtggatgtcc     1080

agggtgttct tgatggtttt cttcagcacg tcggcaggca gccgcttcag cagatggatg     1140

gtctgcttgt tgttcttccg gttgctctcg atgtagctga tcacggtgtt gtacacccgg     1200

atcttggggc tgttaggttc ctcgttgtcc cgcagcttct tgatgtcgtc gctgttcagc     1260

tctgtcagca gcttgctcat ggccacgcag gcgctctgct tggtgatgtt gttgatgctg     1320

ccgatatagc tttccagcac gcccacaacg cccagggcat attcctcggt tctatccagt     1380

tcggcggctc cgctgatctc gctcagggtg tcgatgctct tatccatgga cttcaggatt     1440

ctgttcagca tgaagttctg ccgcacgagc agagcgtgag gaggccactc gaagtagttg     1500

tggctgaagt ggcatctctt gccgttcagg cagtggcctc tgatctcgaa cttgcagggg     1560

ttccgtctgc tcatggtggc ggtattgtct tcggtaccgc acgaagttcc tattcggaag     1620

ttcctattct tcaaatagta taggaacttc cgctctgacc agctgcatta acatggtcat     1680

agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg     1740

gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc     1800

gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca     1860

aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt     1920

ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc     1980

tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc     2040

tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc     2100

ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact     2160

tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg     2220

ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta     2280

tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca     2340

aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa     2400

aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg     2460

aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc     2520

ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg     2580

acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat     2640

ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg     2700

gccccagtgc tgcaatgata ccgcgagaac cacgctcacc ggctccagat ttatcagcaa     2760

taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca     2820

tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc     2880

gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt     2940

cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa     3000

aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat     3060

cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct     3120

tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga     3180

gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag     3240

tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga     3300

gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca     3360

ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg     3420

cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc     3480

agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     3540

gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt     3600

aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg     3660

caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtggcc gctacagggc     3720

gctcccattc gccattcagg ctgcgcaact gttgggaagg gcgtttcggt gcgggcctct     3780

tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg     3840

ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcga cgtaatacga     3900

ctcactatag ggcgaattgg cggaaggccg tcaaggccgc atgaattcgc taccgggagt     3960

tggtaggtaa gtggctgggg ccagagactg gctctt                               3996


<210>  12
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  turboRFP_GT_F1 1.I.7

<400>  12
gagaggccat tctcagatgg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  turboRFP_GT_R1 1.I.8

<400>  13
cgggcatctt caggttcttg                                                   20


<210>  14
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  turboRFP_probe_FAM 1.I.9

<400>  14
ctacctgcac tgctccttca agacc                                             25


<210>  15
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAC_TCRA-promoter_F1 10.A.10

<400>  15
gacttgtcac tggatttaga gtctct                                            26


<210>  16
<211>  22
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAC_probe (HEX) 10.B.6

<400>  16
atccagaacc ctgaccctgc cg                                                22


<210>  17
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAC-TCRA-ex1-F1 10.A.9

<400>  17
ctgatcctct tgtcccacag ata                                               23


<210>  18
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AAVS1_GT_F5 15.F.9

<400>  18
actctgccct ctaacgctg                                                    19


<210>  19
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AMPN-TMD_GT_R1 19.E.7

<400>  19
gctgatgtag aagcccttgg                                                   20


<210>  20
<211>  876
<212>  DNA
<213>  Artificial sequence

<220>
<223>  SurfaceTag with Barcode FLAG-MYC-HA

<400>  20
atggaggagg gacagtacag cgagatcgag gagctgccca ggagaaggtg ctgcaggaga       60

ggcacccaga tcgtgctgct gggactggtg accgctgctc tgtgggcagg actgctgaca      120

ttgctgttgc tgtggcactg ggacaccacc cagagcctga agcagctgga ggagggaggt      180

cctggatccg gaacaggtgg ctctggcact ggaggatcag gtccaggtgg atctatggtg      240

aagcagatcg agagcaagac tgctttccag gaagccttgg acgctgcagg tgataagctt      300

gtagtagttg acttctcagc aacgtggtct ggaccttcca agatgatcaa gcctttcttc      360

cattccctct ctgagaagta ttccaacgtg atattccttg aagtagatgt ggatgactct      420

caggatgttg cttcagagtg tgaagtcaaa tccatgccaa cattccagtt cttcaagaag      480

ggacagaagg tgggtgaatt ttctggagcc aataaggaga agcttgaagc caccattaac      540

gagttggtcg gtggttcctc tacatccggt ggatctggat ctggtgatta caaggatcat      600

gatggagatt ataaagacca cgatattgat tataaggacg atgacgataa gggaggatat      660

ccgtatgatg taccagacta cgctggatac ccttacgatg tacctgatta tgccggatac      720

ccctatgacg taccggacta tgcgggagga gaacaaaagc tcatctctga ggaggacctt      780

ggggagcaga agctaatcag tgaagaggac ctcggagagc agaaattgat tagcgaagag      840

gatcttggcg ggcaccatca tcatcaccac cactaa                                876


<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ORF-AM_GT_F2

<400>  21
ttctgtagct ccattggcag                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ORF-AM_GT_R1

<400>  22
atccgtatgg tgacaagacg                                                   20


<210>  23
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TRAC-TCRA-ex1-F1

<400>  23
gacttgtcac tggatttaga gtctct                                            26


