                               SEQUENCE LISTING

<110> OXFORD NANOPORE TECHNOLOGIES LIMITED
 
<120> POLYNUCLEOTIDE SYNTHESIS METHOD, KIT AND SYSTEM

<130> N414125WO

<140> To be assigned
<141> 2019-07-19

<150> GB1811811.7
<151> 2018-07-19

<160> 80    

<170> PatentIn version 3.5

<210> 1
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 1
gcgacaggtg actgcagc                                                     18


<210> 2
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 2
cacatcacgt cgtagtcngc tgcagtcacc tgtcgc                                 36


<210> 3
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 3
cacatcacgt cgtagtcngc tgcagtcacc tgtcgc                                 36


<210> 4
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 4
gcgacaggtg actgcagct                                                    19


<210> 5
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 5
gcgacaggtg actgcagctg actacgacgt gatgtg                                 36


<210> 6
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-deoxyinosine 

<400> 6
cacatcacgt cgtagtnagc tgcagtcacc tgtcgc                                 36


<210> 7
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 7
gctgcagtca cctgtcgc                                                     18


<210> 8
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 8
gcgacaggtg actgcagct                                                    19


<210> 9
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-deoxyinosine 

<400> 9
cacatcacgt cgtagtna                                                     18


<210> 10
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 10
gcgacaggtg actgcagc                                                     18


<210> 11
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 11
cacatcacgt cgtagtcngc tgcagtcacc tgtcgc                                 36


<210> 12
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 12
cgactacgac gtgatgtg                                                     18


<210> 13
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> Deoxyinosine 

<400> 13
cacatcacgt cgtagtcngc tgcagtcacc tgtcgc                                 36


<210> 14
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 14
gcgacaggtg actgcagct                                                    19


<210> 15
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 15
gcgacaggtg actgcagctg actacgacgt gatgtg                                 36


<210> 16
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 16
tgactacgac gtgatgtg                                                     18


<210> 17
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-deoxyinosine 

<400> 17
cacatcacgt cgtagtnagc tgcagtcacc tgtcgc                                 36


<210> 18
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 18
gctgcagtca cctgtcgc                                                     18


<210> 19
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 19
gcgacaggtg actgcagct                                                    19


<210> 20
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-deoxyinosine 

<400> 20
cacatcacgt cgtagtna                                                     18


<210> 21
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 21
cactacgacg tgatgtg                                                      17


<210> 22
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 22
gcgacaggtg actgcagc                                                     18


<210> 23
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 23
cacatcacgt cgtagtcnag ctgcagtcac ctgtcgc                                37


<210> 24
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 24
cacatcacgt cgtagtcngg ctgcagtcac ctgtcgc                                37


<210> 25
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 25
cacatcacgt cgtagtcnag ctgcagtcac ctgtcgc                                37


<210> 26
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-deoxyinosine 

<400> 26
cacatcacgt cgtagtcnag ctgcagtcac ctgtcgc                                37


<210> 27
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 27
tcgactacga cgtgatgtg                                                    19


<210> 28
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 28
ccgactacga cgtgatgtg                                                    19


<210> 29
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 29
acgactacga cgtgatgtg                                                    19


<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 30
gcgactacga cgtgatgtg                                                    19


<210> 31
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (18)..(18)
<223> Deoxyinosine 

<400> 31
cacatcacgt cgtagtcnag ctgcagtcac ctgtcgc                                37


<210> 32
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 32
gcgacaggtg actgcagct                                                    19


<210> 33
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 33
gcgacaggtg actgcagctg actacgacgt gatgtg                                 36


<210> 34
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 34
tcgactacga cgtgatgtg                                                    19


<210> 35
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 35
gctgcagtca cctgtcgc                                                     18


<210> 36
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 36
gcgacaggtg actgcagct                                                    19


<210> 37
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-deoxyinosine 

<400> 37
cacatcacgt cgtagtnga                                                    19


<210> 38
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 38
ccactacgac gtgatgtg                                                     18


<210> 39
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 39
gcgacaggtg actgcagc                                                     18


<210> 40
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 40
cacatcacgt cgtagtcagc tgcagtcacc tgtcgc                                 36


<210> 41
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 41
cacatcacgt cgtagtcggc tgcagtcacc tgtcgc                                 36


<210> 42
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 42
cacatcacgt cgtagtctgc tgcagtcacc tgtcgc                                 36


<210> 43
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 43
cacatcacgt cgtagtccgc tgcagtcacc tgtcgc                                 36


<210> 44
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (58)..(58)
<223> Tamra-dT

<400> 44
tcgactacga cgtgactttt agtcacgtcg tagtcnagct gcagtcacct gctgcttntt       60

gcagcaggtg actgcagc                                                     78


<210> 45
<211> 79
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (58)..(58)
<223> Tamra-dT

<400> 45
tcgactacga cgtgactttt agtcacgtcg tagtcnagct gcagtcacct gctgcttntt       60

gcagcaggtg actgcagct                                                    79


<210> 46
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (21)..(21)
<223> Tamra-dT phosphate

<400> 46
gctgcagtca cctgctgctt nttgcagcag gtgactgcag ct                          42


<210> 47
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<400> 47
ccgactacga cgtgactttt agtcacgtcg tagtcnga                               38


<210> 48
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (58)..(58)
<223> Tamra-dT 

<400> 48
tcgactacga cgtgactttt agtcacgtcg tagtcnagct gcagtcacct gctgcttntt       60

gcagcaggtg actgcagc                                                     78


<210> 49
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<400> 49
ccgactacga cgtgactttt agtcacgtcg tagtcnga                               38


<210> 50
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (38)..(38)
<223> Tamra-dT 

<400> 50
agtcacgtcg tagtcnagct gcagtcacct gctgcttntt gcagcaggtg actgcagc         58


<210> 51
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 51
tcgactacga cgtgact                                                      17


<210> 52
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<400> 52
agtcacgtcg tagtcnga                                                     18


<210> 53
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 53
ccgactacga cgtgact                                                      17


<210> 54
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (35)..(35)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (57)..(57)
<223> Tamra-dT 

<400> 54
cgactacgac gtgactttta gtcacgtcgt agtcnagctg cagtcacctg ctgcttnttg       60

cagcaggtga ctgcagct                                                     78


<210> 55
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (35)..(35)
<223> 2'-deoxyinosine 

<400> 55
cgactacgac gtgactttta gtcacgtcgt agtcnaa                                37


<210> 56
<211> 78
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (35)..(35)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (52)..(52)
<223> Tamra-dT 

<220>
<221> modified_base
<222> (57)..(57)
<223> Biotin-dT

<400> 56
cgactacgac gtgactttta gtcacgtcgt agtcnagctg cagtcacctg cngcttnttg       60

cagcaggtga ctgcagct                                                     78


<210> 57
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (36)..(36)
<223> 2'-deoxyinosine 

<400> 57
ccgactacga cgtgactttt agtcacgtcg tagtcnga                               38


<210> 58
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (60)..(60)
<223> 2'-deoxyinosine 

<400> 58
acgagtgacc tggttttttt tttttttttt tttttttttt tttttttacc aggtcactcn       60

tg                                                                      62


<210> 59
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (33)..(33)
<223> Tamra-dT

<220>
<221> modified_base
<222> (38)..(38)
<223> Biotin-dT

<400> 59
agtcacgtcg tagtcnagct gcagtcacct gcngcttntt gcagcaggtg actgcagc         58


<210> 60
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<400> 60
agtcacgtcg tagtcnaa                                                     18


<210> 61
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<400> 61
agtcacgtcg tagtcnagct gcagtcacct gctgcttttt tttttttttg cagcaggtga       60

ctgcagc                                                                 67


<210> 62
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<400> 62
agtcacgtcg tagtcnagct gcagtcacct gctgcttttt tttttttttg cagcaggtga       60

ctgcagc                                                                 67


<210> 63
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (38)..(38)
<223> 5''-thiophosphate-Sp9-Sp9-Sp9-5-methylC

<400> 63
agtcacgtcg tagtcnagct gcagtcacct gctgcttctt gcagcaggtg actgcagc         58


<210> 64
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (38)..(38)
<223> 5''-phosphate-Sp9-Sp9-Sp9-5-methylC 

<400> 64
agtcacgtcg tagtcnagct gcagtcacct gctgcttctt gcagcaggtg actgcagc         58


<210> 65
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<220>
<221> modified_base
<222> (38)..(38)
<223> 5''-thiophosphate-Sp9-Sp9-Sp9-5-methylC

<400> 65
agtcacgtcg tagtcnagct gcagtcacct gctgcttctt gcagcaggtg actgcagct        59


<210> 66
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (38)..(38)
<223> 5''-thiophosphate-Sp9-Sp9-Sp9-5-methylC 

<400> 66
agtcacgtcg tagtcgagct gcagtcacct gctgcttctt gcagcaggtg actgcagct        59


<210> 67
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-deoxyinosine 

<400> 67
agtcacgtcg tagtcnaa                                                     18

<210>  68
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer sequence - figure 51

<400>  68
gcgacaggtg actgcagc                                                     18


<210>  69
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  template sequence - figure 51

<400>  69
gctgcagtca cctgtcgc                                                     18

<210>  70
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Hairpin 1 base overhang T - figure 55"

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  TAMRA-dT

<400>  70
tgctgtagtc accatctgcn tttttagcag atggtgacta cagc                        44


<210>  71
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Hairpin 1 base overhang C - figure 55"


<220>
<221>  modified_base
<222>  (20)..(20)
<223>  TAMRA-dT

<400>  71
cgctgtagtc accatctgcn tttttagcag atggtgacta cagc                        44


<210>  72
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Hairpin 2 base overhang - figure 55"


<220>
<221>  modified_base
<222>  (21)..(21)
<223>  TAMRA-dT

<400>  72
acgctgtagt caccatctgc ntttttagca gatggtgact acagc                       45


<210>  73
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Hairpin 3 base overhang - figure 55"


<220>
<221>  modified_base
<222>  (22)..(22)
<223>  TAMRA-dT


<400>  73
cacgctgtag tcaccatctg cntttttagc agatggtgac tacagc                      46


<210>  74
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Hairpin 4 base overhang - figure 55"


<220>
<221>  modified_base
<222>  (23)..(23)
<223>  TAMRA-dT


<400>  74
tcacgctgta gtcaccatct gcntttttag cagatggtga ctacagc                     47


<210>  75
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: In strand - figure 55"


<220>
<221>  modified_base
<222>  (16)..(16)
<223>  2'-deoxyinosine


<400>  75
agtcacgtcg tagtcncg                                                     18


<210>  76
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helper strand - overhang T - figure 55

<400>  76
acgcgactac gacgtgact                                                    19


<210>  77
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helper strand - overhang C - figure 55

<400>  77
gcgcgactac gacgtgact                                                    19


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helper strand - 2 base overhang - figure 55

<400>  78
gtcgcgacta cgacgtgact                                                   20


<210>  79
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helper strand - 3 base overhang - figure 55

<400>  79
gtgcgcgact acgacgtgac t                                                 21


<210>  80
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Helper strand - 4 base overhang - figure 55

<400>  80
gtgacgcgac tacgacgtga ct                                                22



