                         SEQUENCE LISTING

<110>  DUKE UNIVERSITY
       LI, Qi-Jing
       WANG, Xiao-Fan
       CHONG, Mengyang
       CHEN, Rui
 
<120>  ANTI-LYPD3 CAR T-CELL THERAPY FOR THE TREATMENT OF CANCER

<130>  19-1384-WO

<150>  US 62/700,942
<151>  2018-07-20

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1
atggctctgc ccgtcaccgc cctgctgctg cctctggctc tgctgctgca cgccgcacga       60

cccgaagtcc agctgctgga aagtggggga ggcctggtgc agccaggagg ctctctgagg      120

ctgagctgcg cagcatccgg cttcaccttt agcaacgcat ggatgtcctg ggtgaggcag      180

gcacctggca agggcctgga gtgggtgtcc tacatcagct cctctggctc tacaatctac      240

tatgccgaca gcgtgaaggg caggttcacc atctctagag ataacagcaa gaatacactg      300

tatctgcaga tgaattccct gagggccgag gacaccgccg tgtactattg cgccagagag      360

ggcctgtggg cctttgataa gtggggccag ggcaccctgg tgacagtgag ctccggagga      420

ggaggatctg gcggaggagg cagcggcggc ggcggctctc agagcgtgct gacccagcca      480

ccttccgcct ctggaacacc aggccagaga gtgaccatct cctgtacagg ctctagctcc      540

aacatcggcg ccggctacgt ggtgcactgg tatcagcagc tgccaggaac cgcaccaaag      600

ctgctgatct acgacaacaa taagcggcct agcggcgtgc cagatcgctt cagcggctcc      660

aagtctggca caagcgcctc cctggcaatc tccggcctgc ggtctgagga cgaggcagat      720

tactattgtg ccgcctttga cgatcgcctg aatggacccg tgttcggagg agggactaaa      780

ctgaccgtgc tgggacaggc                                                  800


<210>  2
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2
ttcgtgccgg tcttcctgcc agcgaagccc accacgacgc cagcgccgcg accaccaaca       60

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      120

gcggggggcg cagtgcacac gagggggctg gacttcgcct gtgatatcta catctgggcg      180

cccttggccg ggacttgtgg ggtccttctc ctgtcactgg ttatcaccct ttactgcaac      240

cacaggaac                                                              249


<210>  3
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc       60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc      120

tcc                                                                    123


<210>  4
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4
cgtttctctg ttgttaaacg gggcagaaag aagctcctgt atatattcaa acaaccattt       60

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa      120

gaagaaggag gatgtgaact g                                                141


<210>  5
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210>  6
<211>  216
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
actactacca agccagtgct gcgaactccc tcacctgtgc accctaccgg gacatctcag       60

ccccagagac cagaagattg tcggccccgt ggctcagtga aggggaccgg attggacttc      120

gcctgtgata tttacatctg ggcacccttg gccggaatct gcgtggccct tctgctgtcc      180

ttgatcatca ctctcatctg ctaccacagg agccga                                216


<210>  7
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
aatagtagaa ggaacagact ccttcaagtg actaccatga acatgactcc ccggaggcct       60

gggctcactc gaaagcctta ccagccctac gcccctgcca gagactttgc agcgtaccgc      120

ccc                                                                    123


<210>  8
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
aaatggatca ggaaaaaatt cccccacata ttcaagcaac catttaagaa gaccactgga       60

gcagctcaag aggaagatgc ttgtagctgc cgatgtccac aggaagaaga aggaggagga      120

ggaggctatg agctg                                                       135


<210>  9
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9
agagcaaaat tcagcaggag tgcagagact gctgccaacc tgcaggaccc caaccagctc       60

tacaatgagc tcaatctagg gcgaagagag gaatatgacg tcttggagaa gaagcgggct      120

cgcgatccag agatgggagg caaacagcag aggaggagga acccccagga aggcgtatac      180

aatgcactgc agaaagacaa gatggcagaa gcctacagtg agatcggcac aaaaggcgag      240

aggcggagag gcaaggggca cgatggcctt taccagggtc tcagcactgc caccaaggac      300

acctatgatg ccctgcatat gcagaccctg gcccctcgc                             339


<210>  10
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Asn Ala Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Ser Gly Ser Thr Ile Tyr 
65                  70                  75                  80  


Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser 
                85                  90                  95      


Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ala Arg Glu Gly Leu Trp Ala Phe Asp Lys Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Ser Val Leu Thr Gln Pro 
145                 150                 155                 160 


Pro Ser Ala Ser Gly Thr Pro Gly Gln Arg Val Thr Ile Ser Cys Thr 
                165                 170                 175     


Gly Ser Ser Ser Asn Ile Gly Ala Gly Tyr Val Val His Trp Tyr Gln 
            180                 185                 190         


Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu Ile Tyr Asp Asn Asn Lys 
        195                 200                 205             


Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Thr 
    210                 215                 220                 


Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg Ser Glu Asp Glu Ala Asp 
225                 230                 235                 240 


Tyr Tyr Cys Ala Ala Phe Asp Asp Arg Leu Asn Gly Pro Val Phe Gly 
                245                 250                 255     


Gly Gly Thr Lys Leu Thr Val Leu Gly Gln 
            260                 265     


<210>  11
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    50                  55                  60                  


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
65                  70                  75                  80  


His Arg Asn 
            


<210>  12
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  13
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  15
<211>  72
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15

Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro Thr 
1               5                   10                  15      


Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly Ser 
            20                  25                  30          


Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
        35                  40                  45              


Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu Ser Leu Ile Ile Thr 
    50                  55                  60                  


Leu Ile Cys Tyr His Arg Ser Arg 
65                  70          


<210>  16
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16

Asn Ser Arg Arg Asn Arg Leu Leu Gln Val Thr Thr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
        35                  40      


<210>  17
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17

Lys Trp Ile Arg Lys Lys Phe Pro His Ile Phe Lys Gln Pro Phe Lys 
1               5                   10                  15      


Lys Thr Thr Gly Ala Ala Gln Glu Glu Asp Ala Cys Ser Cys Arg Cys 
            20                  25                  30          


Pro Gln Glu Glu Glu Gly Gly Gly Gly Gly Tyr Glu Leu 
        35                  40                  45  


<210>  18
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18

Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn Leu Gln Asp 
1               5                   10                  15      


Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn Ala Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr Leu Ala Pro 
            100                 105                 110         


Arg 
    


<210>  19
<211>  1642
<212>  DNA
<213>  Homo sapiens

<400>  19
acatccaggg gggacgccaa gggagcagga cggagccatg gaccccgcca ggaaagcagg       60

tgcccaggcc atgatctgga ctgcaggctg gctgctgctg ctgctgcttc gcggaggagc      120

gcaggccctg gagtgctaca gctgcgtgca gaaagcagat gacggatgct ccccgaacaa      180

gatgaagaca gtgaagtgcg cgccgggcgt ggacgtctgc accgaggccg tgggggcggt      240

ggagaccatc cacggacaat tctcgctggc agtgcggggt tgcggttcgg gactccccgg      300

caagaatgac cgcggcctgg atcttcacgg gcttctggcg ttcatccagc tgcagcaatg      360

cgctcaggat cgctgcaacg ccaagctcaa cctcacctcg cgggcgctcg acccggcagg      420

taatgagagt gcatacccgc ccaacggcgt ggagtgctac agctgtgtgg gcctgagccg      480

ggaggcgtgc cagggtacat cgccgccggt cgtgagctgc tacaacgcca gcgatcatgt      540

ctacaagggc tgcttcgacg gcaacgtcac cttgacggca gctaatgtga ctgtgtcctt      600

gcctgtccgg ggctgtgtcc aggatgaatt ctgcactcgg gatggagtaa caggcccagg      660

gttcacgctc agtggctcct gttgccaggg gtcccgctgt aactctgacc tccgcaacaa      720

gacctacttc tcccctcgaa tcccacccct tgtccggctg ccccctccag agcccacgac      780

tgtggcctca accacatctg tcaccacttc tacctcggcc ccagtgagac ccacatccac      840

caccaaaccc atgccagcgc caaccagtca gactccgaga cagggagtag aacacgaggc      900

ctcccgggat gaggagccca ggttgactgg aggcgccgct ggccaccagg accgcagcaa      960

ttcagggcag tatcctgcaa aaggggggcc ccagcagccc cataataaag gctgtgtggc     1020

tcccacagct ggattggcag cccttctgtt ggccgtggct gctggtgtcc tactgtgagc     1080

ttctccacct ggaaatttcc ctctcaccta cttctctggc cctgggtacc cctcttctca     1140

tcacttcctg ttcccaccac tggactgggc tggcccagcc cctgtttttc caacattccc     1200

cagtatcccc agcttctgct gcgctggttt gcggctttgg gaaataaaat accgttgtat     1260

atattctgcc aggggtgttc tagctttttg aggacagctc ctgtatcctt ctcatccttg     1320

tctctccgct tgtcctcttg tgatgttagg acagagtgag agaagtcagc tgtcacgggg     1380

aaggtgagag agaggatgct aagcttccta ctcactttct cctagccagc ctggactttg     1440

gagcgtgggg tgggtgggac aatggctccc cactctaagc actgcctccc ctactccccg     1500

catctttggg gaatcggttc cccatatgtc ttccttacta gactgtgagc tcctcgaggg     1560

cagggaccgt gccttatgtc tgtgtgtgat cagtttctgg cacataaatg cctcaataaa     1620

gatttaatta ctttgtatag tg                                              1642


<210>  20
<211>  346
<212>  PRT
<213>  Homo sapiens

<400>  20

Met Asp Pro Ala Arg Lys Ala Gly Ala Gln Ala Met Ile Trp Thr Ala 
1               5                   10                  15      


Gly Trp Leu Leu Leu Leu Leu Leu Arg Gly Gly Ala Gln Ala Leu Glu 
            20                  25                  30          


Cys Tyr Ser Cys Val Gln Lys Ala Asp Asp Gly Cys Ser Pro Asn Lys 
        35                  40                  45              


Met Lys Thr Val Lys Cys Ala Pro Gly Val Asp Val Cys Thr Glu Ala 
    50                  55                  60                  


Val Gly Ala Val Glu Thr Ile His Gly Gln Phe Ser Leu Ala Val Arg 
65                  70                  75                  80  


Gly Cys Gly Ser Gly Leu Pro Gly Lys Asn Asp Arg Gly Leu Asp Leu 
                85                  90                  95      


His Gly Leu Leu Ala Phe Ile Gln Leu Gln Gln Cys Ala Gln Asp Arg 
            100                 105                 110         


Cys Asn Ala Lys Leu Asn Leu Thr Ser Arg Ala Leu Asp Pro Ala Gly 
        115                 120                 125             


Asn Glu Ser Ala Tyr Pro Pro Asn Gly Val Glu Cys Tyr Ser Cys Val 
    130                 135                 140                 


Gly Leu Ser Arg Glu Ala Cys Gln Gly Thr Ser Pro Pro Val Val Ser 
145                 150                 155                 160 


Cys Tyr Asn Ala Ser Asp His Val Tyr Lys Gly Cys Phe Asp Gly Asn 
                165                 170                 175     


Val Thr Leu Thr Ala Ala Asn Val Thr Val Ser Leu Pro Val Arg Gly 
            180                 185                 190         


Cys Val Gln Asp Glu Phe Cys Thr Arg Asp Gly Val Thr Gly Pro Gly 
        195                 200                 205             


Phe Thr Leu Ser Gly Ser Cys Cys Gln Gly Ser Arg Cys Asn Ser Asp 
    210                 215                 220                 


Leu Arg Asn Lys Thr Tyr Phe Ser Pro Arg Ile Pro Pro Leu Val Arg 
225                 230                 235                 240 


Leu Pro Pro Pro Glu Pro Thr Thr Val Ala Ser Thr Thr Ser Val Thr 
                245                 250                 255     


Thr Ser Thr Ser Ala Pro Val Arg Pro Thr Ser Thr Thr Lys Pro Met 
            260                 265                 270         


Pro Ala Pro Thr Ser Gln Thr Pro Arg Gln Gly Val Glu His Glu Ala 
        275                 280                 285             


Ser Arg Asp Glu Glu Pro Arg Leu Thr Gly Gly Ala Ala Gly His Gln 
    290                 295                 300                 


Asp Arg Ser Asn Ser Gly Gln Tyr Pro Ala Lys Gly Gly Pro Gln Gln 
305                 310                 315                 320 


Pro His Asn Lys Gly Cys Val Ala Pro Thr Ala Gly Leu Ala Ala Leu 
                325                 330                 335     


Leu Leu Ala Val Ala Ala Gly Val Leu Leu 
            340                 345     


