﻿                         序列表

<110>  江南大学

<120>  表达磷脂酶D的重组菌株及应用

<160>  6

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  540
<212>  PRT
<213>  （人工序列）

<400>  1
Met Ala Arg His Pro Arg Lys Arg Arg Ser Ala Leu Val Pro Arg Thr 
1               5                   10                  15      
Ala Val Pro Ala Leu Val Ala Val Leu Leu Pro Val Ala Pro Ala Ser 
            20                  25                  30          
Ala Asp Thr Gly Ala Thr Pro Ala Thr Pro His Leu Asp Ala Val Glu 
        35                  40                  45              
Gln Thr Leu Arg Gln Val Ser Pro Gly Leu Glu Gly Arg Val Trp Glu 
    50                  55                  60                  
Arg Thr Ala Gly Asn Thr Leu Asp Ala Ser Thr Pro Gly Gly Ala Asp 
65                  70                  75                  80  
Trp Leu Leu Gln Thr Pro Gly Cys Trp Gly Asp Ala Thr Cys Ala Asp 
                85                  90                  95      
Arg Pro Gly Thr Arg Gln Leu Leu Val Lys Met Thr Glu Asn Val Ser 
            100                 105                 110         
Arg Ala Thr Glu Ser Val Asp Ile Ser Thr Leu Ala Pro Phe Pro Asn 
        115                 120                 125             
Gly Ala Phe Gln Asp Ala Val Val Ser Gly Leu Lys Ala Ser Val Ala 
    130                 135                 140                 
Ser Gly His Gln Pro Lys Val Arg Ile Leu Val Gly Ala Ala Pro Ile 
145                 150                 155                 160 
Tyr His Leu Asn Val Val Pro Ser Lys Tyr Arg Asp Glu Leu Val Glu 
                165                 170                 175     
Lys Leu Gly Lys Asp Ala Ala Lys Val Thr Leu Asn Val Ala Ser Met 
            180                 185                 190         
Thr Thr Ser Lys Thr Ala Phe Ser Trp Asn His Ser Lys Leu Leu Val 
        195                 200                 205             
Val Asp Gly Arg Ser Ala Ile Thr Gly Gly Ile Asn Gly Trp Lys Asp 
    210                 215                 220                 
Asp Tyr Leu Asp Thr Thr His Pro Val Ser Asp Val Asp Leu Ala Leu 
225                 230                 235                 240 
Ser Gly Pro Ala Ala Ala Ser Ala Gly Lys Tyr Leu Asn Glu Leu Trp 
                245                 250                 255     
Ser Trp Thr Cys Arg Asn Lys Asn Asn Ile Ala Ser Val Trp Phe Ala 
            260                 265                 270         
Ser Ser Asn Gly Ala Gly Cys Met Pro Thr Leu Glu Pro Ala Ala Ser 
        275                 280                 285             
Ala Glu Ala Pro Arg Gly Asp Val Pro Val Ile Ala Val Gly Gly Leu 
    290                 295                 300                 
Gly Val Gly Ile Gln Arg Asp Asp Pro Ala Ser Arg Phe Arg Pro Thr 
305                 310                 315                 320 
Leu Pro Thr Ala Pro Asp Thr Lys Cys Val Val Ala Leu His Asp Asn 
                325                 330                 335     
Thr Asn Ala Asp Arg Asp Tyr Asp Thr Val Asn Pro Glu Glu Ser Ala 
            340                 345                 350         
Leu Arg Ala Leu Ile Ser Ser Ala Asp Arg His Ile Glu Ile Ser Gln 
        355                 360                 365             
Gln Asp Leu Asn Ala Thr Cys Pro Pro Leu Pro Arg Tyr Asp Ile Arg 
    370                 375                 380                 
Val Tyr Asp Ala Leu Ala Ala Lys Met Ala Ala Gly Val Lys Val Arg 
385                 390                 395                 400 
Ile Val Val Ser Asp Pro Ala Asn Arg Gly Ala Val Gly Ser Gly Gly 
                405                 410                 415     
Tyr Ser Gln Ile Lys Ser Leu Thr Glu Ile Ser Asp Thr Leu Arg Asp 
            420                 425                 430         
Arg Leu Ala Leu Arg Thr Gly Asp Gln Ala Ser Ala Arg Thr Ala Met 
        435                 440                 445             
Cys Ser Asn Leu Gln Leu Ala Thr Ala Arg Ser Ser Thr Ser Pro Lys 
    450                 455                 460                 
Trp Ala Asp Glu His Pro Tyr Ala Gln His His Lys Leu Ile Ser Val 
465                 470                 475                 480 
Asp Gly Ser Ala Phe Tyr Ile Gly Ser Lys Asn Leu Tyr Pro Ala Trp 
                485                 490                 495     
Leu Gln Asp Phe Gly Tyr Val Val Glu Ser Pro Ala Ala Ala Lys Gln 
            500                 505                 510         
Leu Asp Thr His Leu Leu Ala Pro Gln Trp Gln Phe Ser Arg Asp Thr 
        515                 520                 525             
Ala Thr Val Asp Tyr Glu Arg Gly Ile Cys Gln Asp 
    530                 535                 540 

<210>  2
<211>  1623
<212>  DNA
<213>  （人工序列）

<400>  2
atggcacgtc atccgcgcaa acgtcgctcc gcactggtgc cgcgtaccgc agttccggca        60
ctggtggctg ttctgctgcc ggtggcgccg gcctcagcag acaccggtgc aacgccggca       120
accccgcacc tggatgcggt ggaacagacc ctgcgccaag tttcgccggg cctggaaggt       180
cgtgtttggg aacgcacggc tggtaacacc ctggacgcaa gcaccccggg cggtgcagat       240
tggctgctgc agacgccggg ttgctggggt gacgcaacgt gtgctgatcg tccgggcacc       300
cgccaactgc tggttaaaat gacggaaaac gtctctcgtg caaccgaaag tgtcgacatt       360
tccacgctgg cgccgtttcc gaatggtgcg ttccaggatg ccgttgttag tggcctgaaa       420
gcgagcgttg cctctggtca tcaaccgaaa gtccgtattc tggtgggcgc ggccccgatc       480
tatcacctga atgtcgtgcc gagcaaatac cgcgacgaac tggtggaaaa actgggtaaa       540
gatgcagcta aagttaccct gaacgtcgca tcaatgacca cgtcgaaaac ggcttttagc       600
tggaatcatt ctaaactgct ggttgtcgat ggccgttctg cgattaccgg cggtatcaac       660
ggttggaaag atgactatct ggataccacg caccctgtga gtgatgttga cctggcactg       720
tctggtccgg cagcagcaag tgctggtaaa tacctgaacg aactgtggtc gtggacctgc       780
cgcaataaaa acaatattgc gagcgtgtgg tttgccagct ctaatggcgc aggttgtatg       840
ccgaccctgg aaccggctgc atccgctgaa gcaccgcgtg gcgatgtccc ggtgattgca       900
gtcggcggtc tgggcgtggg tatccagcgt gatgacccgg ccagccgttt ccgtccgacg       960
ctgccgaccg caccggacac caaatgcgtg gttgcgctgc atgataacac caatgccgat      1020
cgtgactacg atacggtgaa cccggaagaa tctgcactgc gtgctctgat cagttccgcg      1080
gaccgccaca ttgaaatcag tcagcaagat ctgaatgcca cctgtccgcc gctgccgcgt      1140
tatgacattc gcgtgtacga tgcgctggcc gcaaaaatgg ctgcgggcgt taaagtccgt      1200
atcgtcgtga gcgatccggc aaaccgtggt gctgttggta gtggcggtta ttcccagatt      1260
aaatcactga cggaaatcag cgacaccctg cgtgatcgtc tggcactgcg taccggtgat      1320
caggcctccg cacgtacggc aatgtgctca aacctgcaac tggctacggc gcgctcatcg      1380
acctctccga aatgggcaga cgaacatccg tatgctcagc atcacaaact gatttcggtg      1440
gatggcagcg cattttatat cggttccaaa aatctgtacc cggcctggct gcaggatttc      1500
ggctacgttg tcgaatcacc ggccgcagct aaacaactgg atacccacct gctggcgccg      1560
cagtggcaat tcagtcgtga caccgccacg gttgattatg aacgcggtat ttgtcaggat      1620
taa                                                                    1623

<210>  3
<211>  96
<212>  DNA
<213>  （人工序列）

<400>  3
atgaaaaaaa gaaagaggcg aaactttaaa aggttcattg cagcattttt agtgttggct        60
ttaatgattt cattagtgcc agccgatgta ctagca                                  96

<210>  4
<211>  15
<212>  DNA
<213>  （人工序列）

<400>  4
agaaggagat atacc                                                         15

<210>  5
<211>  372
<212>  DNA
<213>  （人工序列）

<400>  5
atttttatca acgaaattta tttcaatcat tgtatttctc gaccccgctg tcgcgatcgt        60
gctcgatacc gtcttcacag gcttccgccc tgacctctat caaacgcttg gcatcgtaat       120
gatctttgcg ggcatggcct tgacgcttgt caggaggcag gggaaggcga atgtgacagc       180
tgagggtacg gatattgaac aaatacaata aaaaatgtaa aaaggcctat gcggcctttt       240
tttgttttag gtcaattgac tctcgctaat ccttaaaata agataaattt tctagaaaaa       300
tattgtaatg atatttcagt ctagttaaga ttattgagta aatattactt ttattacaaa       360
aggagagagg aa                                                           372

<210>  6
<211>  8351
<212>  DNA
<213>  （人工序列）

<400>  6
gaattcgcta gcgagctccc atgggcggcc gcctcgaggg aaccagatct ccgcggctta        60
agctgcagaa gcttctgttt tggcggatga gagaagattt tcagcctgat acagattaaa       120
tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc       180
ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg       240
tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa       300
agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa       360
tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg       420
cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt       480
tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca       540
tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc       600
aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc       660
acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt       720
acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt       780
ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg       840
ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact       900
caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg       960
ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga      1020
aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg      1080
aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa      1140
tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac      1200
aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc      1260
cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca      1320
ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga      1380
gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta      1440
agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc      1500
atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc      1560
cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt      1620
cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac      1680
cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct      1740
tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact      1800
tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg      1860
ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata      1920
aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga      1980
cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag      2040
ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg      2100
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac      2160
ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca      2220
acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg      2280
cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc      2340
gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga      2400
tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca      2460
gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga      2520
ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg      2580
tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca      2640
gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg      2700
gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc      2760
cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg      2820
tttggtcact tgatgcctcc gtgtaagggg gaatttctgt tcatgggggt aatgataccg      2880
atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg      2940
gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact      3000
cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag      3060
catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga      3120
ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg      3180
cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg      3240
caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc      3300
caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg      3360
gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct      3420
ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg      3480
tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc      3540
ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga      3600
tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc      3660
cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc      3720
cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca      3780
gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga      3840
aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata      3900
ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga      3960
cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg      4020
cggcgacgat agtccattgt caacaacaag acccatcata gtttgccccc gcgacattga      4080
ccataaattc atcgcacaaa atatcgaacg gggtttatgc cgcttttagt gggtgcgaag      4140
aatagtctgc tcattacccg cgaacaccgc cgcattcaga tcacgcttag tagcgtcccc      4200
atgagtaggc agaaccgcgt ccaagtccac atcatccata acgatcatgc acggggtgga      4260
atccacaccc agacttgcca gcacctcatt agcgacacgt tgcgcagcgg ccacgtcctt      4320
agccttatcc acgcaatcta gaacgtactg cctaaccgcg aaatcagact gaatcagttt      4380
ccaatcatcg ggcttcacca aagcaacagc aacgcgggtt gattcgaccc gttccggtgc      4440
ttccagaccg gcgagcttgt acagttcttc ttccatttca cgacgtacat cagcgtctat      4500
gtaatcaatg cccaaagcac gcttagcccc acgtgaccag gacgaacgca ggtttttaga      4560
accaacctca tactcacgcc accgagccac caaaacagcg tccatatcct cgccggcgtc      4620
gctttgatcg gccaacatat ccaacatctg aaacggcgtg tacgacccct tagacgcggt      4680
tttagtagcg gagccagtca gttcctgaga catgccctta gcgaggtagg ttgccatttt      4740
cgcagcgtct ccaccccagg tagacacctg atcaagtttg accccgtgct cacgcagtgg      4800
cgcgtccata ccggccttaa ccacaccagc agaccagcgg gaaaacatgg aatcctcaaa      4860
cgccttgagt tcatcgtcag acagtggacg atccaagaac aacagcatgt tgcggtgcaa      4920
gtgccaaccg ttcgcccaag agtctgtgac ctcatagtca ctataggtgt gctccacccc      4980
gtaccgtgca cgttctttct tccactgaga tgttttcacc atcgaagagt acgcagtctt      5040
aatacccgct tcaacctgcg caaatgactg tgagcggttg tgtcgaacag tgcccacaaa      5100
catcatgagc gcgccacccg ccgccaagtg attcttagta gcaatagcca gctcaatgcg      5160
gcgttcgccc atgacttcca attcagccag aggtgacccc cagcgagagt gagagttttg      5220
cagaccctca aactgcgaag caccgttaga cgaccaggac accgcaacag cttcgtccct      5280
gcgccaccta tggcaccccg ccagagcctt actattggtg atcttgtaca tgacgttttg      5340
cctacgccac gccctagcgc gagtgacctt agaaccctca ttgacctgcg gttccttaga      5400
ggtgttcact tctatttcag tgttacctag acccgatgtt gtgcggggtt gcgcagtgcg      5460
agtttgtgcg ggtgttgtgc ccgttgtctt agctagtgct atggttgtca attgaaaccc      5520
cttcgggtta tgtggccccc gtgcatatga gttggtagct cgcacggggg tttgtcttgt      5580
ctagggacta ttaattttta gtggtgtttg gtggccgcct agcttggcta tgcgtgccag      5640
cttacccgta ctcaatgtta aagatttgca tcgacatggg agggttacgt gtccgatacc      5700
tagggggggt atccgcgact aggtgccccg gtgctcactg tctgtaccgg cggggcaagc      5760
cccacacccc gcatggacag ggtggctccg ccccctgcac ccccagcaat ctgcatgtac      5820
atgttttaca cattagcacg acatgactgc atgtgcatgc actgcatgca gactaggtaa      5880
atatgagtat gtacgactag taacaggagc actgcacata atgaatgagt tgcaggacaa      5940
tgtttgctac gcatgcgcat gacatatcgc aggaaagcta ctagagtctt aaagcatggc      6000
aaccaaggca cagctagaac agcaactaca agaagctcaa caggcactac aggcgcagca      6060
agcgcaggca caagccacca tcgaagcact agaagcgcag gcaaaggcta agcccgtcgt      6120
ggtcaccgca cgcgttcctt tggcactacg tgaggacatg aagcgcgcag gcatgcagaa      6180
cggtgaaaac ctccaagagt tcatgatcgc cgcgtttacc gagcggctag aaaagctcac      6240
caccaccgac aacgaggaaa acaatgtcta acccactagt tctctttgcc caccgtgacc      6300
cggtaaatga cgtgacgttc gagtgcattg agcacgccac ctacgacaca ctttcacacg      6360
ctaaagacca gatcaccgcc caaatgcaag ccctagacga agaagccgcc ctactgccct      6420
aatgggtgtt tcatgggtgt ttccctagtg tttcatggtg ttttcaccta agctagggaa      6480
ttgcgcgaga agtctcgcaa aaatcagcaa cccccggaac cacacagttc acgggggttc      6540
ttctatgcca gaaatcagaa aggggaacca gtgaacgacc ccgaatattg gatcacagcg      6600
cagcaggtcg ccgcccgcgt agctctcacc ccggccacca ttaaaaagtg ggcaaacgag      6660
ggaaaaatca ccgcatacaa gatcggcaag tccgtccgat tcaaagcatc agacgtagac      6720
gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg      6780
catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt      6840
gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag      6900
tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa      6960
gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc      7020
tgtggcgccg gtgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt      7080
atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat      7140
cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa      7200
aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat      7260
cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct      7320
cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga      7380
atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt      7440
catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac      7500
gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca      7560
ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct      7620
ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga      7680
taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct      7740
catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat      7800
cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc      7860
atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg      7920
tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt      7980
ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc      8040
gtagaaaaga tcaaagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatccgg      8100
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg      8160
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt      8220
tctggataat gttttttgcg ccgacatcat aacggtaccg gcaaatattc tgaaatgagc      8280
tgttgacaat taatcatcgg ctggaaccat gtgtggaatt gtgagcggat aacaatttca      8340
cacaggaaac a                                                           8351

