                         SEQUENCE LISTING

<110>  Wisconsin Alumni Research Foundation
       Shusta, Eric V.
       Stutz, Charles C.
 
<120>  BLOOD BRAIN BARRIER SELECTIVE ANTIBODIES AND METHODS OF USE

<130>  960296.02515

<150>  62/702,796
<151>  2018-07-24

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  244
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(244)
<223>  scFv4

<400>  1

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Gly Gly Ser Gly Gly Ser Thr Tyr Tyr Thr Glu Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ala Trp Asp Ser Tyr Ser Arg Lys Pro Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Asn Phe Met Leu Thr Gln Asp Pro Ala 
    130                 135                 140                 


Val Ser Val Ala Leu Gly Gln Thr Val Arg Ile Thr Cys Gln Gly Asp 
145                 150                 155                 160 


Ser Leu Lys Ser His Tyr Ala Ser Trp Tyr His Gln Lys Pro Gly Gln 
                165                 170                 175     


Ala Pro Leu Leu Val Ile Tyr Asp Asn Asn Asn Arg Pro Ser Gly Val 
            180                 185                 190         


Pro Asp Arg Phe Ser Gly Ser Arg Ser Gly Thr Ser Ala Ser Leu Ala 
        195                 200                 205             


Ile Thr Gly Val Gln Ala Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser 
    210                 215                 220                 


Tyr Asp Ser Ser Leu Arg Gly Ser Arg Val Phe Gly Thr Gly Thr Lys 
225                 230                 235                 240 


Val Thr Val Leu 
                


<210>  2
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDRH1 region of scFv4

<400>  2

Gly Phe Thr Phe Ser Ser Tyr Ala 
1               5               


<210>  3
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDRH2 region of scFv4

<400>  3

Ile Gly Gly Ser Gly Gly Ser Thr 
1               5               


<210>  4
<211>  11
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(11)
<223>  CDRH3 region of scFv4

<400>  4

Ala Trp Asp Ser Tyr Ser Arg Lys Pro Asp Tyr 
1               5                   10      


<210>  5
<211>  6
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  CDRL1 region of scFv4

<400>  5

Ser Leu Lys Ser His Tyr 
1               5       


<210>  6
<211>  3
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  CDRL2 region of scFv4

<400>  6

Asp Asn Asn 
1           


<210>  7
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  CDRL3 region of scFv4

<400>  7

Gln Ser Tyr Asp Ser Ser Leu Arg Gly Ser Arg Val 
1               5                   10          


<210>  8
<211>  248
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(248)
<223>  svFv40

<400>  8

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met Ser Trp Val Arg Leu Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Arg Ser Thr Asn Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Phe Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu His Ser Glu Asp Ser Ser Gly Trp Arg Val Phe Asp Ile 
            100                 105                 110         


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Arg Ser Gln Pro Glu Leu Thr Gln 
    130                 135                 140                 


Asp Pro Ala Val Ser Gly Ser Pro Gly Gln Ser Ile Thr Ile Ser Tyr 
145                 150                 155                 160 


Thr Gly Thr Ser Ser Asp Val Gly Gly Tyr Asn Tyr Val Ser Trp Tyr 
                165                 170                 175     


Gln Gln His Pro Gly Lys Ala Pro Lys Leu Met Ile Tyr Glu Val Ser 
            180                 185                 190         


Asn Arg Pro Ser Gly Val Ser Asn Arg Phe Ser Gly Ser Lys Ser Gly 
        195                 200                 205             


Asn Thr Ala Ser Leu Thr Ile Ser Gly Leu Gln Ala Glu Asp Glu Ala 
    210                 215                 220                 


Asp Tyr Tyr Cys Ser Ser Tyr Thr Asp Ser Asn Val Val Phe Gly Gly 
225                 230                 235                 240 


Gly Thr Lys Leu Thr Val Leu Gly 
                245             


<210>  9
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDRH1 region of svFv40

<400>  9

Gly Phe Thr Phe Ser Ser Tyr Gly 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDRH2 region of svFv40

<400>  10

Ile Ser Gly Ser Gly Arg Ser Thr 
1               5               


<210>  11
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  CDRH3 region of svFv40

<400>  11

Leu His Ser Glu Asp Ser Ser Gly Trp Arg Val Phe Asp 
1               5                   10              


<210>  12
<211>  9
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  CDRL1 region of svFv40

<400>  12

Ser Ser Asp Val Gly Gly Tyr Asn Tyr 
1               5                   


<210>  13
<211>  3
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  CDRL2 region of svFv40

<400>  13

Glu Val Ser 
1           


<210>  14
<211>  9
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(9)
<223>  CDRL3 region of svFv40

<400>  14

Ser Ser Tyr Thr Asp Ser Asn Val Val 
1               5                   


<210>  15
<211>  734
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(734)
<223>  scFv4

<400>  15
caggtgcagc tggtggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc       60

tcctgtgcag cctctggatt cacctttagc agctatgcca tgagctgggt ccgccaggct      120

ccagggaagg ggctggagtg ggtctcagct attggtggta gtggtggtag cacatactac      180

acagagtccg tgaagggccg gttcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gaaagcctgg      300

gattcgtata gtaggaaacc tgactactgg ggccagggca ccctggtcac cgtctcctca      360

ggtggaggcg gttcaggcgg aggtggctct ggcggtggcg gatcgaattt tatgctgact      420

caggaccctg ctgtgtctgt ggccttggga cagacagtca ggatcacatg ccaaggagac      480

agcctcaaaa gccattatgc aagctggtac catcagaagc caggacaggc ccctctactt      540

gtcatctatg ataacaacaa tcggccctca ggggtccctg accgattctc tggctccagg      600

tctggcacct cagcctccct ggccatcact ggggtccagg ctgaagatga ggctgactat      660

tactgccagt catatgacag cagcctgagg ggttcgaggg tcttcggaac tgggaccaag      720

gtcaccgtcc tagt                                                        734


<210>  16
<211>  24
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  CDRH1 region of scFv4

<400>  16
ggattcacct ttagcagcta tgcc                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  CDRH2 region of scFv4

<400>  17
attggtggta gtggtggtag caca                                              24


<210>  18
<211>  39
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(39)
<223>  CDRH3 region of scFv4

<400>  18
gcgaaagcct gggattcgta tagtaggaaa cctgactac                              39


<210>  19
<211>  18
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  CDRL1 region of scFv4

<400>  19
agcctcaaaa gccattat                                                     18


<210>  20
<211>  9
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  CDRL2 region of scFv4

<400>  20
gataacaac                                                                9


<210>  21
<211>  36
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(36)
<223>  CDRL3 region of scFv4

<400>  21
cagtcatatg acagcagcct gaggggttcg agggtc                                 36


<210>  22
<211>  744
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(744)
<223>  scFv40

<400>  22
caggtgcagc tgcaggagtc ggggggaggc ttggtacagg cgggggggtc cctcagactc       60

tcctgtgcag cctctggatt cacctttagc agctatggca tgagctgggt ccgcctggct      120

ccggggaagg ggctggagtg ggtctcagct attagtggta gtggacgtag cacaaactac      180

gcagactccg tgaagggccg gttcaccatc ttcagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agccgaggac acggccgtat attactgtgc gaaactccat      300

agtgaggata gcagtggctg gagagttttt gatatctggg gccaagggac aatggtcacc      360

gtctcttcag gtggaggcgg ttcaggcgga ggtggctctg gcggtggcag atcgcagcct      420

gagctgactc aggaccctgc tgtgtctggg tctcctggac agtcgatcac catctcctac      480

actggaacca gcagtgacgt tggtggttat aactatgtct cctggtacca acagcaccca      540

ggcaaagccc ccaaactcat gatttatgag gtcagtaatc ggccctcagg ggtttctaat      600

cgcttctctg gctccaagtc tggcaacacg gcctccctga ccatctctgg gctccaggct      660

gaggacgagg ctgattatta ctgcagctca tatacagaca gcaatgtggt attcggcgga      720

gggaccaagc tgaccgtcct aggt                                             744


<210>  23
<211>  24
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  CDRH1 region of svFv40

<400>  23
ggattcacct ttagcagcta tggc                                              24


<210>  24
<211>  24
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  CDRH2 region of svFv40

<400>  24
attagtggta gtggacgtag caca                                              24


<210>  25
<211>  45
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(45)
<223>  CDRH3 region of svFv40

<400>  25
gcgaaactcc atagtgagga tagcagtggc tggagagttt ttgat                       45


<210>  26
<211>  27
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  CDRL1 region of svFv40

<400>  26
agcagtgacg ttggtggtta taactat                                           27


<210>  27
<211>  9
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  CDRL2 region of svFv40

<400>  27
gaggtcagt                                                                9


<210>  28
<211>  27
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  CDRL3 region of svFv40

<400>  28
agctcatata cagacagcaa tgtggta                                           27


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for the amplification of human CDR-H3's

<400>  29
gaaccgcctc cacctgagg                                                    19


<210>  30
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for the amplification of scFv

<400>  30
gaattttctg tatgaggttt tgctaaa                                           27


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Foward primer for the amplification of scFv


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  Forward primer for amplification of scFv

<400>  31
tttttggaga ttttcaacgt ga                                                22


<210>  32
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  CDR-H3 region of scFv clone 1

<400>  32

Gly Ala Leu Gln Ser Gly Ser Tyr Tyr Pro Pro Gly Tyr 
1               5                   10              


<210>  33
<211>  5
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(5)
<223>  CDR-H3 region of scFv clone 2

<400>  33

Asp Asn Gly Glu Tyr 
1               5   


<210>  34
<211>  8
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(8)
<223>  CDR-H3 region of scFv clone 6

<400>  34

Ala Asp Gly Gly Asn Ser Asp Tyr 
1               5               


<210>  35
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  CDR-H3 region of scFv clone 8

<400>  35

Gly Ser Met Val Arg Gly Pro Tyr Pro Arg Phe Asp Pro 
1               5                   10              


<210>  36
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  CDR-H3 region of scFv clone 10

<400>  36

Glu Thr Gly Ala Tyr Tyr Tyr Tyr Gly Met Asp Val 
1               5                   10          


<210>  37
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(13)
<223>  CDR-H3 region of scFv clone 11

<400>  37

Asp Arg Trp Leu Gln Asn Trp Glu Arg Pro Phe Asp Tyr 
1               5                   10              


<210>  38
<211>  12
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(12)
<223>  CDR-H3 region of scFv clone 17

<400>  38

Tyr Tyr Tyr Asp Ser Ser Ala Phe Met Thr Gly Asn 
1               5                   10          


<210>  39
<211>  16
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(16)
<223>  CDR-H3 region of scFv clone 18

<400>  39

Asp Leu Tyr Asp Ser Ser Gly Asn Leu His Gly Asn Trp Phe Asp Pro 
1               5                   10                  15      


<210>  40
<211>  21
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(21)
<223>  CDR-H3 region of scFv clone 23

<400>  40

Asp Leu His Leu Ser Ile Ala Ala Ala Gly Thr Gly Val Phe Lys Thr 
1               5                   10                  15      


Pro Leu Arg Asp Tyr 
            20      


<210>  41
<211>  10
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(10)
<223>  CDR-H3 region of scFv clone 59

<400>  41

Arg Leu Gly Tyr Ser Ala Pro Gly Asp Tyr 
1               5                   10  


