                         SEQUENCE LISTING

<110>  H. Lee Moffitt Cancer Center and Research Institute, Inc.
 
<120>  CAR T-CELLS FOR THE TREATMENT OF BONE METASTATIC CANCER

<130>  320803-2260

<150>  US 62/715,504
<151>  2018-08-07

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  1

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    50                  55                  60                  


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
65                  70                  75                  80  


His Arg Asn Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro 
                85                  90                  95      


Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu 
            100                 105                 110         


Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala 
        115                 120                 125             


Cys Ser Pro Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    130                 135                 140                 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
145                 150                 155                 160 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                165                 170                 175     


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            180                 185                 190         


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        195                 200                 205             


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    210                 215                 220                 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
225                 230                 235                 240 


Pro Pro Arg 
            


<210>  2
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Arg Glu Glu Glu Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg 
1               5                   10                  15  


<210>  3
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
    50                  55                  60                  


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
65                  70                  75                  80  


His Arg Asn Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro 
                85                  90                  95      


Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Lys Pro Glu Pro Ala 
        115                 120                 125             


Cys Ser Pro Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    130                 135                 140                 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
145                 150                 155                 160 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                165                 170                 175     


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            180                 185                 190         


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        195                 200                 205             


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    210                 215                 220                 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
225                 230                 235                 240 


Pro Pro Arg 
            


<210>  4
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Glu Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly 
        35                  40                  45              


Gly Ser Ile Ser Ser Gly Gly Tyr Tyr Trp Ile Trp Ile Arg Gln His 
    50                  55                  60                  


Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Asn Gly Asn 
65                  70                  75                  80  


Thr Tyr Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Met Ser Val Asp 
                85                  90                  95      


Thr Ser Lys Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Gly Ile Thr Met Ile Arg 
        115                 120                 125             


Gly Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Val Ser Ala Ser 
                165                 170                 175     


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Arg Gly Ile Ser 
            180                 185                 190         


Ser Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Thr Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
225                 230                 235                 240 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala Tyr Ser Phe 
                245                 250                 255     


Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Ala Ala 
            260                 265                 270         


Phe Val 
        


<210>  5
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Met Val Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gln Val Gln Leu Gln Glu Ser Gly Pro Gly 
            20                  25                  30          


Leu Val Lys Pro Ser Gln Thr Leu Ser Leu Thr Cys Thr Val Ser Gly 
        35                  40                  45              


Gly Ser Ile Ser Ser Gly Gly Tyr Tyr Trp Ile Trp Ile Arg Gln His 
    50                  55                  60                  


Pro Gly Lys Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Tyr Asn Gly Asn 
65                  70                  75                  80  


Thr Tyr Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr Met Ser Val Asp 
                85                  90                  95      


Thr Ser Lys Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala 
            100                 105                 110         


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asp Gly Ile Thr Met Ile Arg 
        115                 120                 125             


Gly Tyr Tyr Tyr Gly Met Asp Val Trp Gly Gln Gly Thr Thr Val Thr 
    130                 135                 140                 


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
145                 150                 155                 160 


Gly Ser Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Val Ser Ala Ser 
                165                 170                 175     


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Arg Gly Ile Ser 
            180                 185                 190         


Ser Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        195                 200                 205             


Leu Ile Tyr Thr Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe 
    210                 215                 220                 


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
225                 230                 235                 240 


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala Tyr Ser Phe 
                245                 250                 255     


Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Ala Ala Ala 
            260                 265                 270         


Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
        275                 280                 285             


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
    290                 295                 300                 


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
305                 310                 315                 320 


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
                325                 330                 335     


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn 
            340                 345                 350         


His Arg Asn Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro 
        355                 360                 365             


Val Glu Pro Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu 
    370                 375                 380                 


Gly Ser Thr Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala 
385                 390                 395                 400 


Cys Ser Pro Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                405                 410                 415     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            420                 425                 430         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        435                 440                 445             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    450                 455                 460                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
465                 470                 475                 480 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                485                 490                 495     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            500                 505                 510         


Pro Pro Arg 
        515 


