                         SEQUENCE LISTING

<110>  Emory University
 
<120>  Methods of Managing Nucleic Acid Replication, Expression, and 
       Cleavage Using CRISPR Associated Nucleases

<130>  18094 PCT

<150>  US 62/718,719
<151>  2019-08-14

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  1
tata                                                                     4


<210>  2
<211>  8
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<400>  2
nnnngatt                                                                 8


<210>  3
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is a, c, g, t or u

<400>  3
nnagaa                                                                   6


<210>  4
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, t or u

<400>  4
naaaac                                                                   6


<210>  5
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  5
gctttatata aattagctgt aatggaggta ttaaatat                               38


<210>  6
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  6
atatttaata cctccattac agctaattta tataaagc                               38


<210>  7
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  7
agcaaatgaa aaagattagc tgtaagtttc agttgttaga ttatttggta tgtac            55


<210>  8
<211>  75
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  8
gtaccaaata attaatgctc tgtaatcatt taaaagtatt ttgaacggac ctctgtttga       60

cacgtctgaa taact                                                        75


<210>  9
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  9
gctatttact tagtaattag ctgtaatgga gaaatccaaa tgttaata                    48


<210>  10
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  10
tattaacatt tggatttctc cattacagct aattactaag taaatagc                    48


<210>  11
<211>  7
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  11
aggaggu                                                                  7


<210>  12
<211>  7
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  12
accaugg                                                                  7


<210>  13
<211>  7
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, t or u

<400>  13
tatanan                                                                  7


<210>  14
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  14
tataat                                                                   6


<210>  15
<211>  5
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  15
ccaat                                                                    5


<210>  16
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is a, c, g, t or u

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n is a, c, g, t or u

<400>  16
nngnnt                                                                   6


<210>  17
<211>  7
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<400>  17
nnnnaca                                                                  7


<210>  18
<211>  8
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, t or u

<400>  18
nnnngatt                                                                 8


<210>  19
<211>  4
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n is a, c, g, t or u

<400>  19
tttn                                                                     4


<210>  20
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  20

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  21
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  21

Gly Gly Gly Gly Thr 
1               5   


<210>  22
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  22

Gly Gly Gly Pro Pro Pro 
1               5       


<210>  23
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  23

Gly Gly Gly Ala Pro Pro Pro 
1               5           


<210>  24
<211>  1629
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  24

Met Asn Phe Lys Ile Leu Pro Ile Ala Ile Asp Leu Gly Val Lys Asn 
1               5                   10                  15      


Thr Gly Val Phe Ser Ala Phe Tyr Gln Lys Gly Thr Ser Leu Glu Arg 
            20                  25                  30          


Leu Asp Asn Lys Asn Gly Lys Val Tyr Glu Leu Ser Lys Asp Ser Tyr 
        35                  40                  45              


Thr Leu Leu Met Asn Asn Arg Thr Ala Arg Arg His Gln Arg Arg Gly 
    50                  55                  60                  


Ile Asp Arg Lys Gln Leu Val Lys Arg Leu Phe Lys Leu Ile Trp Thr 
65                  70                  75                  80  


Glu Gln Leu Asn Leu Glu Trp Asp Lys Asp Thr Gln Gln Ala Ile Ser 
                85                  90                  95      


Phe Leu Phe Asn Arg Arg Gly Phe Ser Phe Ile Thr Asp Gly Tyr Ser 
            100                 105                 110         


Pro Glu Tyr Leu Asn Ile Val Pro Glu Gln Val Lys Ala Ile Leu Met 
        115                 120                 125             


Asp Ile Phe Asp Asp Tyr Asn Gly Glu Asp Asp Leu Asp Ser Tyr Leu 
    130                 135                 140                 


Lys Leu Ala Thr Glu Gln Glu Ser Lys Ile Ser Glu Ile Tyr Asn Lys 
145                 150                 155                 160 


Leu Met Gln Lys Ile Leu Glu Phe Lys Leu Met Lys Leu Cys Thr Asp 
                165                 170                 175     


Ile Lys Asp Asp Lys Val Ser Thr Lys Thr Leu Lys Glu Ile Thr Ser 
            180                 185                 190         


Tyr Glu Phe Glu Leu Leu Ala Asp Tyr Leu Ala Asn Tyr Ser Glu Ser 
        195                 200                 205             


Leu Lys Thr Gln Lys Phe Ser Tyr Thr Asp Lys Gln Gly Asn Leu Lys 
    210                 215                 220                 


Glu Leu Ser Tyr Tyr His His Asp Lys Tyr Asn Ile Gln Glu Phe Leu 
225                 230                 235                 240 


Lys Arg His Ala Thr Ile Asn Asp Arg Ile Leu Asp Thr Leu Leu Thr 
                245                 250                 255     


Asp Asp Leu Asp Ile Trp Asn Phe Asn Phe Glu Lys Phe Asp Phe Asp 
            260                 265                 270         


Lys Asn Glu Glu Lys Leu Gln Asn Gln Glu Asp Lys Asp His Ile Gln 
        275                 280                 285             


Ala His Leu His His Phe Val Phe Ala Val Asn Lys Ile Lys Ser Glu 
    290                 295                 300                 


Met Ala Ser Gly Gly Arg His Arg Ser Gln Tyr Phe Gln Glu Ile Thr 
305                 310                 315                 320 


Asn Val Leu Asp Glu Asn Asn His Gln Glu Gly Tyr Leu Lys Asn Phe 
                325                 330                 335     


Cys Glu Asn Leu His Asn Lys Lys Tyr Ser Asn Leu Ser Val Lys Asn 
            340                 345                 350         


Leu Val Asn Leu Ile Gly Asn Leu Ser Asn Leu Glu Leu Lys Pro Leu 
        355                 360                 365             


Arg Lys Tyr Phe Asn Asp Lys Ile His Ala Lys Ala Asp His Trp Asp 
    370                 375                 380                 


Glu Gln Lys Phe Thr Glu Thr Tyr Cys His Trp Ile Leu Gly Glu Trp 
385                 390                 395                 400 


Arg Val Gly Val Lys Asp Gln Asp Lys Lys Asp Gly Ala Lys Tyr Ser 
                405                 410                 415     


Tyr Lys Asp Leu Cys Asn Glu Leu Lys Gln Lys Val Thr Lys Ala Gly 
            420                 425                 430         


Leu Val Asp Phe Leu Leu Glu Leu Asp Pro Cys Arg Thr Ile Pro Pro 
        435                 440                 445             


Tyr Leu Asp Asn Asn Asn Arg Lys Pro Pro Lys Cys Gln Ser Leu Ile 
    450                 455                 460                 


Leu Asn Pro Lys Phe Leu Asp Asn Gln Tyr Pro Asn Trp Gln Gln Tyr 
465                 470                 475                 480 


Leu Gln Glu Leu Lys Lys Leu Gln Ser Ile Gln Asn Tyr Leu Asp Ser 
                485                 490                 495     


Phe Glu Thr Asp Leu Lys Val Leu Lys Ser Ser Lys Asp Gln Pro Tyr 
            500                 505                 510         


Phe Val Glu Tyr Lys Ser Ser Asn Gln Gln Ile Ala Ser Gly Gln Arg 
        515                 520                 525             


Asp Tyr Lys Asp Leu Asp Ala Arg Ile Leu Gln Phe Ile Phe Asp Arg 
    530                 535                 540                 


Val Lys Ala Ser Asp Glu Leu Leu Leu Asn Glu Ile Tyr Phe Gln Ala 
545                 550                 555                 560 


Lys Lys Leu Lys Gln Lys Ala Ser Ser Glu Leu Glu Lys Leu Glu Ser 
                565                 570                 575     


Ser Lys Lys Leu Asp Glu Val Ile Ala Asn Ser Gln Leu Ser Gln Ile 
            580                 585                 590         


Leu Lys Ser Gln His Thr Asn Gly Ile Phe Glu Gln Gly Thr Phe Leu 
        595                 600                 605             


His Leu Val Cys Lys Tyr Tyr Lys Gln Arg Gln Arg Ala Arg Asp Ser 
    610                 615                 620                 


Arg Leu Tyr Ile Met Pro Glu Tyr Arg Tyr Asp Lys Lys Leu His Lys 
625                 630                 635                 640 


Tyr Asn Asn Thr Gly Arg Phe Asp Asp Asp Asn Gln Leu Leu Thr Tyr 
                645                 650                 655     


Cys Asn His Lys Pro Arg Gln Lys Arg Tyr Gln Leu Leu Asn Asp Leu 
            660                 665                 670         


Ala Gly Val Leu Gln Val Ser Pro Asn Phe Leu Lys Asp Lys Ile Gly 
        675                 680                 685             


Ser Asp Asp Asp Leu Phe Ile Ser Lys Trp Leu Val Glu His Ile Arg 
    690                 695                 700                 


Gly Phe Lys Lys Ala Cys Glu Asp Ser Leu Lys Ile Gln Lys Asp Asn 
705                 710                 715                 720 


Arg Gly Leu Leu Asn His Lys Ile Asn Ile Ala Arg Asn Thr Lys Gly 
                725                 730                 735     


Lys Cys Glu Lys Glu Ile Phe Asn Leu Ile Cys Lys Ile Glu Gly Ser 
            740                 745                 750         


Glu Asp Lys Lys Gly Asn Tyr Lys His Gly Leu Ala Tyr Glu Leu Gly 
        755                 760                 765             


Val Leu Leu Phe Gly Glu Pro Asn Glu Ala Ser Lys Pro Glu Phe Asp 
    770                 775                 780                 


Arg Lys Ile Lys Lys Phe Asn Ser Ile Tyr Ser Phe Ala Gln Ile Gln 
785                 790                 795                 800 


Gln Ile Ala Phe Ala Glu Arg Lys Gly Asn Ala Asn Thr Cys Ala Val 
                805                 810                 815     


Cys Ser Ala Asp Asn Ala His Arg Met Gln Gln Ile Lys Ile Thr Glu 
            820                 825                 830         


Pro Val Glu Asp Asn Lys Asp Lys Ile Ile Leu Ser Ala Lys Ala Gln 
        835                 840                 845             


Arg Leu Pro Ala Ile Pro Thr Arg Ile Val Asp Gly Ala Val Lys Lys 
    850                 855                 860                 


Met Ala Thr Ile Leu Ala Lys Asn Ile Val Asp Asp Asn Trp Gln Asn 
865                 870                 875                 880 


Ile Lys Gln Val Leu Ser Ala Lys His Gln Leu His Ile Pro Ile Ile 
                885                 890                 895     


Thr Glu Ser Asn Ala Phe Glu Phe Glu Pro Ala Leu Ala Asp Val Lys 
            900                 905                 910         


Gly Lys Ser Leu Lys Asp Arg Arg Lys Lys Ala Leu Glu Arg Ile Ser 
        915                 920                 925             


Pro Glu Asn Ile Phe Lys Asp Lys Asn Asn Arg Ile Lys Glu Phe Ala 
    930                 935                 940                 


Lys Gly Ile Ser Ala Tyr Ser Gly Ala Asn Leu Thr Asp Gly Asp Phe 
945                 950                 955                 960 


Asp Gly Ala Lys Glu Glu Leu Asp His Ile Ile Pro Arg Ser His Lys 
                965                 970                 975     


Lys Tyr Gly Thr Leu Asn Asp Glu Ala Asn Leu Ile Cys Val Thr Arg 
            980                 985                 990         


Gly Asp Asn Lys Asn Lys Gly Asn  Arg Ile Phe Cys Leu  Arg Asp Leu 
        995                 1000                 1005             


Ala Asp  Asn Tyr Lys Leu Lys  Gln Phe Glu Thr Thr  Asp Asp Leu 
    1010                 1015                 1020             


Glu Ile  Glu Lys Lys Ile Ala  Asp Thr Ile Trp Asp  Ala Asn Lys 
    1025                 1030                 1035             


Lys Asp  Phe Lys Phe Gly Asn  Tyr Arg Ser Phe Ile  Asn Leu Thr 
    1040                 1045                 1050             


Pro Gln  Glu Gln Lys Ala Phe  Arg His Ala Leu Phe  Leu Ala Asp 
    1055                 1060                 1065             


Glu Asn  Pro Ile Lys Gln Ala  Val Ile Arg Ala Ile  Asn Asn Arg 
    1070                 1075                 1080             


Asn Arg  Thr Phe Val Asn Gly  Thr Gln Arg Tyr Phe  Ala Glu Val 
    1085                 1090                 1095             


Leu Ala  Asn Asn Ile Tyr Leu  Arg Ala Lys Lys Glu  Asn Leu Asn 
    1100                 1105                 1110             


Thr Asp  Lys Ile Ser Phe Asp  Tyr Phe Gly Ile Pro  Thr Ile Gly 
    1115                 1120                 1125             


Asn Gly  Arg Gly Ile Ala Glu  Ile Arg Gln Leu Tyr  Glu Lys Val 
    1130                 1135                 1140             


Asp Ser  Asp Ile Gln Ala Tyr  Ala Lys Gly Asp Lys  Pro Gln Ala 
    1145                 1150                 1155             


Ser Tyr  Ser His Leu Ile Asp  Ala Met Leu Ala Phe  Cys Ile Ala 
    1160                 1165                 1170             


Ala Asp  Glu His Arg Asn Asp  Gly Ser Ile Gly Leu  Glu Ile Asp 
    1175                 1180                 1185             


Lys Asn  Tyr Ser Leu Tyr Pro  Leu Asp Lys Asn Thr  Gly Glu Val 
    1190                 1195                 1200             


Phe Thr  Lys Asp Ile Phe Ser  Gln Ile Lys Ile Thr  Asp Asn Glu 
    1205                 1210                 1215             


Phe Ser  Asp Lys Lys Leu Val  Arg Lys Lys Ala Ile  Glu Gly Phe 
    1220                 1225                 1230             


Asn Thr  His Arg Gln Met Thr  Arg Asp Gly Ile Tyr  Ala Glu Asn 
    1235                 1240                 1245             


Tyr Leu  Pro Ile Leu Ile His  Lys Glu Leu Asn Glu  Val Arg Lys 
    1250                 1255                 1260             


Gly Tyr  Thr Trp Lys Asn Ser  Glu Glu Ile Lys Ile  Phe Lys Gly 
    1265                 1270                 1275             


Lys Lys  Tyr Asp Ile Gln Gln  Leu Asn Asn Leu Val  Tyr Cys Leu 
    1280                 1285                 1290             


Lys Phe  Val Asp Lys Pro Ile  Ser Ile Asp Ile Gln  Ile Ser Thr 
    1295                 1300                 1305             


Leu Glu  Glu Leu Arg Asn Ile  Leu Thr Thr Asn Asn  Ile Ala Ala 
    1310                 1315                 1320             


Thr Ala  Glu Tyr Tyr Tyr Ile  Asn Leu Lys Thr Gln  Lys Leu His 
    1325                 1330                 1335             


Glu Tyr  Tyr Ile Glu Asn Tyr  Asn Thr Ala Leu Gly  Tyr Lys Lys 
    1340                 1345                 1350             


Tyr Ser  Lys Glu Met Glu Phe  Leu Arg Ser Leu Ala  Tyr Arg Ser 
    1355                 1360                 1365             


Glu Arg  Val Lys Ile Lys Ser  Ile Asp Asp Val Lys  Gln Val Leu 
    1370                 1375                 1380             


Asp Lys  Asp Ser Asn Phe Ile  Ile Gly Lys Ile Thr  Leu Pro Phe 
    1385                 1390                 1395             


Lys Lys  Glu Trp Gln Arg Leu  Tyr Arg Glu Trp Gln  Asn Thr Thr 
    1400                 1405                 1410             


Ile Lys  Asp Asp Tyr Glu Phe  Leu Lys Ser Phe Phe  Asn Val Lys 
    1415                 1420                 1425             


Ser Ile  Thr Lys Leu His Lys  Lys Val Arg Lys Asp  Phe Ser Leu 
    1430                 1435                 1440             


Pro Ile  Ser Thr Asn Glu Gly  Lys Phe Leu Val Lys  Arg Lys Thr 
    1445                 1450                 1455             


Trp Asp  Asn Asn Phe Ile Tyr  Gln Ile Leu Asn Asp  Ser Asp Ser 
    1460                 1465                 1470             


Arg Ala  Asp Gly Thr Lys Pro  Phe Ile Pro Ala Phe  Asp Ile Ser 
    1475                 1480                 1485             


Lys Asn  Glu Ile Val Glu Ala  Ile Ile Asp Ser Phe  Thr Ser Lys 
    1490                 1495                 1500             


Asn Ile  Phe Trp Leu Pro Lys  Asn Ile Glu Leu Gln  Lys Val Asp 
    1505                 1510                 1515             


Asn Lys  Asn Ile Phe Ala Ile  Asp Thr Ser Lys Trp  Phe Glu Val 
    1520                 1525                 1530             


Glu Thr  Pro Ser Asp Leu Arg  Asp Ile Gly Ile Ala  Thr Ile Gln 
    1535                 1540                 1545             


Tyr Lys  Ile Asp Asn Asn Ser  Arg Pro Lys Val Arg  Val Lys Leu 
    1550                 1555                 1560             


Asp Tyr  Val Ile Asp Asp Asp  Ser Lys Ile Asn Tyr  Phe Met Asn 
    1565                 1570                 1575             


His Ser  Leu Leu Lys Ser Arg  Tyr Pro Asp Lys Val  Leu Glu Ile 
    1580                 1585                 1590             


Leu Lys  Gln Ser Thr Ile Ile  Glu Phe Glu Ser Ser  Gly Phe Asn 
    1595                 1600                 1605             


Lys Thr  Ile Lys Glu Met Leu  Gly Met Lys Leu Ala  Gly Ile Tyr 
    1610                 1615                 1620             


Asn Glu  Thr Ser Asn Asn 
    1625                 


<210>  25
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  25

Pro Ile Ala Ile Asp Leu Gly Val Lys Asn Thr Gly Val Phe Ser Ala 
1               5                   10                  15      


Phe Tyr Gln Lys 
            20  


<210>  26
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  26

Met Asn Asn Arg Thr Ala Arg Arg His Gln Arg Arg Gly Ile Asp Arg 
1               5                   10                  15      


Lys Gln Leu Val Lys 
            20      


<210>  27
<211>  34
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  27

Glu Trp Asp Lys Asp Thr Gln Gln Ala Ile Ser Phe Leu Phe Asn Arg 
1               5                   10                  15      


Arg Gly Phe Ser Phe Ile Thr Asp Gly Tyr Ser Pro Glu Tyr Leu Asn 
            20                  25                  30          


Ile Val 
        


<210>  28
<211>  35
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  28

Lys Asn Ile Val Asp Asp Asn Trp Gln Asn Ile Lys Gln Val Leu Ser 
1               5                   10                  15      


Ala Lys His Gln Leu His Ile Pro Ile Ile Thr Glu Ser Asn Ala Phe 
            20                  25                  30          


Glu Phe Glu 
        35  


<210>  29
<211>  36
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  29

Leu Asp His Ile Ile Pro Arg Ser His Lys Lys Tyr Gly Thr Leu Asn 
1               5                   10                  15      


Asp Glu Ala Asn Leu Ile Cys Val Thr Arg Gly Asp Asn Lys Asn Lys 
            20                  25                  30          


Gly Asn Arg Ile 
        35      


<210>  30
<211>  31
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  30

Ala Lys Gly Asp Lys Pro Gln Ala Ser Tyr Ser His Leu Ile Asp Ala 
1               5                   10                  15      


Met Leu Ala Phe Cys Ile Ala Ala Asp Glu His Arg Asn Asp Gly 
            20                  25                  30      


