                         SEQUENCE LISTING

<110>  THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY
       Muthumani, Kar
       Weiner, David B
 
<120>  VACCINES AGAINST NIPAH VIRUS, AND METHODS OF USING SAME

<130>  206193-0007-00WO

<150>  US 62/720,393
<151>  2018-08-21

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  1635
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of a consensus NiV-Fusion (F) immunogen

<400>  1
gtggtcatcc tggataagag atgctactgt aacctgctga tcctgatcct gatgatcagc       60

gagtgctccg tgggcatcct gcactacgag aagctgtcca agatcggcct ggtgaagggc      120

gtgacccgga agtataagat caagtctaat cccctgacaa aggacatcgt gatcaagatg      180

atccctaacg tgtctaatat gagccagtgt accggcagcg tgatggagaa ctacaagacc      240

cgcctgaatg gcatcctgac acccatcaag ggcgccctgg agatctataa gaacaataca      300

cacgacctgg tgggcgatgt gaggctggca ggcgtgatca tggcaggagt ggcaatcgga      360

atcgcaaccg cagcacagat cacagcagga gtggccctgt atgaggccat gaagaacgcc      420

gacaacatca ataagctgaa gagctccatc gagtccacca atgaggccgt ggtgaagctg      480

caggagacag ccgagaagac agtgtacgtg ctgacagccc tgcaggacta tatcaacacc      540

aatctggtgc ctacaatcga taagatctct tgcaagcaga ccgagctgag cctggacctg      600

gccctgagca agtacctgtc cgatctgctg ttcgtgtttg gcccaaacct gcaggacccc      660

gtgagcaatt ccatgacaat ccaggccatc agccaggcct tcggcggcaa ctacgagaca      720

ctgctgagaa cactgggcta tgccaccgag gactttgacg atctgctgga gtctgatagc      780

atcacaggcc agatcatcta tgtggacctg tctagctact atatcatcgt gcgggtgtac      840

ttcccaatcc tgaccgagat ccagcaggcc tatatccagg agctgctgcc cgtgagcttc      900

aacaatgata actctgagtg gatcagcatc gtgcctaact tcatcctggt gaggaacacc      960

ctgatctcta atatcgagat cggcttttgc ctgatcacaa agagaagcgt gatctgtaac     1020

caggactacg ccacccctat gacaaacaat atgcgggagt gcctgaccgg ctccacagag     1080

aagtgtcctc gggagctggt ggtgtcctct cacgtgccac gcttcgccct gtccaacggc     1140

gtgctgtttg ccaattgcat ctctgtgacc tgccagtgtc agaccacagg cagggccatc     1200

tcccagtctg gcgagcagac cctgctgatg atcgataaca ccacatgtcc aacagccgtg     1260

ctgggcaatg tgatcatctc cctgggcaag tacctgggca gcgtgaacta taattccgag     1320

ggaatcgcaa tcggccctcc cgtgttcacc gacaaggtgg atatcagctc ccagatctct     1380

agcatgaacc agtccctgca gcagtctaag gactacatca aggaggccca gcgcctgctg     1440

gataccgtga atccatccct gatctctatg ctgagcatga tcatcctgta tgtgctgtcc     1500

atcgcctctc tgtgcatcgg cctgatcacc ttcatcagct ttatcatcgt ggagaagaag     1560

cggaacacat actcccgcct ggaggacagg agagtgaggc ccacctcctc tggcgatctg     1620

tactatatcg gcaca                                                      1635


<210>  2
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a consensus NiV-Fusion (F) immunogen

<400>  2

Val Val Ile Leu Asp Lys Arg Cys Tyr Cys Asn Leu Leu Ile Leu Ile 
1               5                   10                  15      


Leu Met Ile Ser Glu Cys Ser Val Gly Ile Leu His Tyr Glu Lys Leu 
            20                  25                  30          


Ser Lys Ile Gly Leu Val Lys Gly Val Thr Arg Lys Tyr Lys Ile Lys 
        35                  40                  45              


Ser Asn Pro Leu Thr Lys Asp Ile Val Ile Lys Met Ile Pro Asn Val 
    50                  55                  60                  


Ser Asn Met Ser Gln Cys Thr Gly Ser Val Met Glu Asn Tyr Lys Thr 
65                  70                  75                  80  


Arg Leu Asn Gly Ile Leu Thr Pro Ile Lys Gly Ala Leu Glu Ile Tyr 
                85                  90                  95      


Lys Asn Asn Thr His Asp Leu Val Gly Asp Val Arg Leu Ala Gly Val 
            100                 105                 110         


Ile Met Ala Gly Val Ala Ile Gly Ile Ala Thr Ala Ala Gln Ile Thr 
        115                 120                 125             


Ala Gly Val Ala Leu Tyr Glu Ala Met Lys Asn Ala Asp Asn Ile Asn 
    130                 135                 140                 


Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val Lys Leu 
145                 150                 155                 160 


Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu Gln Asp 
                165                 170                 175     


Tyr Ile Asn Thr Asn Leu Val Pro Thr Ile Asp Lys Ile Ser Cys Lys 
            180                 185                 190         


Gln Thr Glu Leu Ser Leu Asp Leu Ala Leu Ser Lys Tyr Leu Ser Asp 
        195                 200                 205             


Leu Leu Phe Val Phe Gly Pro Asn Leu Gln Asp Pro Val Ser Asn Ser 
    210                 215                 220                 


Met Thr Ile Gln Ala Ile Ser Gln Ala Phe Gly Gly Asn Tyr Glu Thr 
225                 230                 235                 240 


Leu Leu Arg Thr Leu Gly Tyr Ala Thr Glu Asp Phe Asp Asp Leu Leu 
                245                 250                 255     


Glu Ser Asp Ser Ile Thr Gly Gln Ile Ile Tyr Val Asp Leu Ser Ser 
            260                 265                 270         


Tyr Tyr Ile Ile Val Arg Val Tyr Phe Pro Ile Leu Thr Glu Ile Gln 
        275                 280                 285             


Gln Ala Tyr Ile Gln Glu Leu Leu Pro Val Ser Phe Asn Asn Asp Asn 
    290                 295                 300                 


Ser Glu Trp Ile Ser Ile Val Pro Asn Phe Ile Leu Val Arg Asn Thr 
305                 310                 315                 320 


Leu Ile Ser Asn Ile Glu Ile Gly Phe Cys Leu Ile Thr Lys Arg Ser 
                325                 330                 335     


Val Ile Cys Asn Gln Asp Tyr Ala Thr Pro Met Thr Asn Asn Met Arg 
            340                 345                 350         


Glu Cys Leu Thr Gly Ser Thr Glu Lys Cys Pro Arg Glu Leu Val Val 
        355                 360                 365             


Ser Ser His Val Pro Arg Phe Ala Leu Ser Asn Gly Val Leu Phe Ala 
    370                 375                 380                 


Asn Cys Ile Ser Val Thr Cys Gln Cys Gln Thr Thr Gly Arg Ala Ile 
385                 390                 395                 400 


Ser Gln Ser Gly Glu Gln Thr Leu Leu Met Ile Asp Asn Thr Thr Cys 
                405                 410                 415     


Pro Thr Ala Val Leu Gly Asn Val Ile Ile Ser Leu Gly Lys Tyr Leu 
            420                 425                 430         


Gly Ser Val Asn Tyr Asn Ser Glu Gly Ile Ala Ile Gly Pro Pro Val 
        435                 440                 445             


Phe Thr Asp Lys Val Asp Ile Ser Ser Gln Ile Ser Ser Met Asn Gln 
    450                 455                 460                 


Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala Gln Arg Leu Leu 
465                 470                 475                 480 


Asp Thr Val Asn Pro Ser Leu Ile Ser Met Leu Ser Met Ile Ile Leu 
                485                 490                 495     


Tyr Val Leu Ser Ile Ala Ser Leu Cys Ile Gly Leu Ile Thr Phe Ile 
            500                 505                 510         


Ser Phe Ile Ile Val Glu Lys Lys Arg Asn Thr Tyr Ser Arg Leu Glu 
        515                 520                 525             


Asp Arg Arg Val Arg Pro Thr Ser Ser Gly Asp Leu Tyr Tyr Ile Gly 
    530                 535                 540                 


Thr 
545 


<210>  3
<211>  1695
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of a consensus NiV-Fusion immunogen operably 
       linked to a sequence encoding an IgE leader and two stop codons

<400>  3
atggactgga cctggattct gttcctggtg gcagcagcaa caagggtgca ctctgtggtc       60

atcctggata agagatgcta ctgtaacctg ctgatcctga tcctgatgat cagcgagtgc      120

tccgtgggca tcctgcacta cgagaagctg tccaagatcg gcctggtgaa gggcgtgacc      180

cggaagtata agatcaagtc taatcccctg acaaaggaca tcgtgatcaa gatgatccct      240

aacgtgtcta atatgagcca gtgtaccggc agcgtgatgg agaactacaa gacccgcctg      300

aatggcatcc tgacacccat caagggcgcc ctggagatct ataagaacaa tacacacgac      360

ctggtgggcg atgtgaggct ggcaggcgtg atcatggcag gagtggcaat cggaatcgca      420

accgcagcac agatcacagc aggagtggcc ctgtatgagg ccatgaagaa cgccgacaac      480

atcaataagc tgaagagctc catcgagtcc accaatgagg ccgtggtgaa gctgcaggag      540

acagccgaga agacagtgta cgtgctgaca gccctgcagg actatatcaa caccaatctg      600

gtgcctacaa tcgataagat ctcttgcaag cagaccgagc tgagcctgga cctggccctg      660

agcaagtacc tgtccgatct gctgttcgtg tttggcccaa acctgcagga ccccgtgagc      720

aattccatga caatccaggc catcagccag gccttcggcg gcaactacga gacactgctg      780

agaacactgg gctatgccac cgaggacttt gacgatctgc tggagtctga tagcatcaca      840

ggccagatca tctatgtgga cctgtctagc tactatatca tcgtgcgggt gtacttccca      900

atcctgaccg agatccagca ggcctatatc caggagctgc tgcccgtgag cttcaacaat      960

gataactctg agtggatcag catcgtgcct aacttcatcc tggtgaggaa caccctgatc     1020

tctaatatcg agatcggctt ttgcctgatc acaaagagaa gcgtgatctg taaccaggac     1080

tacgccaccc ctatgacaaa caatatgcgg gagtgcctga ccggctccac agagaagtgt     1140

cctcgggagc tggtggtgtc ctctcacgtg ccacgcttcg ccctgtccaa cggcgtgctg     1200

tttgccaatt gcatctctgt gacctgccag tgtcagacca caggcagggc catctcccag     1260

tctggcgagc agaccctgct gatgatcgat aacaccacat gtccaacagc cgtgctgggc     1320

aatgtgatca tctccctggg caagtacctg ggcagcgtga actataattc cgagggaatc     1380

gcaatcggcc ctcccgtgtt caccgacaag gtggatatca gctcccagat ctctagcatg     1440

aaccagtccc tgcagcagtc taaggactac atcaaggagg cccagcgcct gctggatacc     1500

gtgaatccat ccctgatctc tatgctgagc atgatcatcc tgtatgtgct gtccatcgcc     1560

tctctgtgca tcggcctgat caccttcatc agctttatca tcgtggagaa gaagcggaac     1620

acatactccc gcctggagga caggagagtg aggcccacct cctctggcga tctgtactat     1680

atcggcacat gataa                                                      1695


<210>  4
<211>  563
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a consensus NiV-Fusion immunogen operably 
       linked to an IgE leader sequence

<400>  4

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Val Val Ile Leu Asp Lys Arg Cys Tyr Cys Asn Leu Leu Ile 
            20                  25                  30          


Leu Ile Leu Met Ile Ser Glu Cys Ser Val Gly Ile Leu His Tyr Glu 
        35                  40                  45              


Lys Leu Ser Lys Ile Gly Leu Val Lys Gly Val Thr Arg Lys Tyr Lys 
    50                  55                  60                  


Ile Lys Ser Asn Pro Leu Thr Lys Asp Ile Val Ile Lys Met Ile Pro 
65                  70                  75                  80  


Asn Val Ser Asn Met Ser Gln Cys Thr Gly Ser Val Met Glu Asn Tyr 
                85                  90                  95      


Lys Thr Arg Leu Asn Gly Ile Leu Thr Pro Ile Lys Gly Ala Leu Glu 
            100                 105                 110         


Ile Tyr Lys Asn Asn Thr His Asp Leu Val Gly Asp Val Arg Leu Ala 
        115                 120                 125             


Gly Val Ile Met Ala Gly Val Ala Ile Gly Ile Ala Thr Ala Ala Gln 
    130                 135                 140                 


Ile Thr Ala Gly Val Ala Leu Tyr Glu Ala Met Lys Asn Ala Asp Asn 
145                 150                 155                 160 


Ile Asn Lys Leu Lys Ser Ser Ile Glu Ser Thr Asn Glu Ala Val Val 
                165                 170                 175     


Lys Leu Gln Glu Thr Ala Glu Lys Thr Val Tyr Val Leu Thr Ala Leu 
            180                 185                 190         


Gln Asp Tyr Ile Asn Thr Asn Leu Val Pro Thr Ile Asp Lys Ile Ser 
        195                 200                 205             


Cys Lys Gln Thr Glu Leu Ser Leu Asp Leu Ala Leu Ser Lys Tyr Leu 
    210                 215                 220                 


Ser Asp Leu Leu Phe Val Phe Gly Pro Asn Leu Gln Asp Pro Val Ser 
225                 230                 235                 240 


Asn Ser Met Thr Ile Gln Ala Ile Ser Gln Ala Phe Gly Gly Asn Tyr 
                245                 250                 255     


Glu Thr Leu Leu Arg Thr Leu Gly Tyr Ala Thr Glu Asp Phe Asp Asp 
            260                 265                 270         


Leu Leu Glu Ser Asp Ser Ile Thr Gly Gln Ile Ile Tyr Val Asp Leu 
        275                 280                 285             


Ser Ser Tyr Tyr Ile Ile Val Arg Val Tyr Phe Pro Ile Leu Thr Glu 
    290                 295                 300                 


Ile Gln Gln Ala Tyr Ile Gln Glu Leu Leu Pro Val Ser Phe Asn Asn 
305                 310                 315                 320 


Asp Asn Ser Glu Trp Ile Ser Ile Val Pro Asn Phe Ile Leu Val Arg 
                325                 330                 335     


Asn Thr Leu Ile Ser Asn Ile Glu Ile Gly Phe Cys Leu Ile Thr Lys 
            340                 345                 350         


Arg Ser Val Ile Cys Asn Gln Asp Tyr Ala Thr Pro Met Thr Asn Asn 
        355                 360                 365             


Met Arg Glu Cys Leu Thr Gly Ser Thr Glu Lys Cys Pro Arg Glu Leu 
    370                 375                 380                 


Val Val Ser Ser His Val Pro Arg Phe Ala Leu Ser Asn Gly Val Leu 
385                 390                 395                 400 


Phe Ala Asn Cys Ile Ser Val Thr Cys Gln Cys Gln Thr Thr Gly Arg 
                405                 410                 415     


Ala Ile Ser Gln Ser Gly Glu Gln Thr Leu Leu Met Ile Asp Asn Thr 
            420                 425                 430         


Thr Cys Pro Thr Ala Val Leu Gly Asn Val Ile Ile Ser Leu Gly Lys 
        435                 440                 445             


Tyr Leu Gly Ser Val Asn Tyr Asn Ser Glu Gly Ile Ala Ile Gly Pro 
    450                 455                 460                 


Pro Val Phe Thr Asp Lys Val Asp Ile Ser Ser Gln Ile Ser Ser Met 
465                 470                 475                 480 


Asn Gln Ser Leu Gln Gln Ser Lys Asp Tyr Ile Lys Glu Ala Gln Arg 
                485                 490                 495     


Leu Leu Asp Thr Val Asn Pro Ser Leu Ile Ser Met Leu Ser Met Ile 
            500                 505                 510         


Ile Leu Tyr Val Leu Ser Ile Ala Ser Leu Cys Ile Gly Leu Ile Thr 
        515                 520                 525             


Phe Ile Ser Phe Ile Ile Val Glu Lys Lys Arg Asn Thr Tyr Ser Arg 
    530                 535                 540                 


Leu Glu Asp Arg Arg Val Arg Pro Thr Ser Ser Gly Asp Leu Tyr Tyr 
545                 550                 555                 560 


Ile Gly Thr 
            


<210>  5
<211>  1803
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of a consensus NiV-Glycoprotein (G) immunogen

<400>  5
ccagccgaga ataagaaggt gaggtttgag aacaccacat ctgacaaggg caagatcccc       60

tctaaagtga tcaagagcta ctatggcacc atggacatca agaagatcaa tgagggcctg      120

ctggatagca agatcctgtc cgccttcaac acagtgatcg ccctgctggg ctccatcgtg      180

atcatcgtga tgaatatcat gatcatccag aactacacca ggtctacaga caatcaggcc      240

gtgatcaagg acgctctgca gggcatccag cagcagatca agggcctggc cgataagatc      300

ggcacagaga tcggccccaa ggtgagcctg atcgacacca gctccaccat cacaatccct      360

gccaacatcg gcctgctggg cagcaagatc tctcagagca ccgcctccat caacgagaat      420

gtgaacgaga agtgcaagtt cacactgccc cctctgaaga tccacgagtg caatatctcc      480

tgtcctaacc cactgccctt tagggagtac agaccacaga ccgagggcgt gtctaatctg      540

gtgggcctgc ccaacaatat ctgtctgcag aagaccagca accagatcct gaagcccaag      600

ctgatctcct atacactgcc tgtggtgggc cagtctggca cctgcatcac agaccctctg      660

ctggccatgg atgagggcta cttcgcctat tctcacctgg agcggatcgg ctcctgttct      720

cgcggcgtga gcaagcagag gatcatcgga gtgggagagg tgctggacag aggcgatgag      780

gtgcctagcc tgttcatgac caacgtgtgg acaccaccca atccaaacac cgtgtaccac      840

tgctccgccg tgtataacaa tgagttttac tacgtgctgt gcgccgtgag caccgtgggc      900

gatcctatcc tgaactccac atactggagc ggctccctga tgatgaccag gctggcagtg      960

aagccaaaga gcaatggcgg cggatataac cagcaccagc tggccctgag atccatcgag     1020

aagggccggt acgataaagt gatgccttat ggcccatccg gcatcaagca gggcgacaca     1080

ctgtacttcc ccgccgtggg ctttctggtg aggaccgagt tcaagtacaa tgactctaac     1140

tgccctatca caaagtgtca gtattctaag ccagagaatt gccgcctgag catgggcatc     1200

aggcccaact ctcactacat cctgcgcagc ggcctgctga agtataatct gagcgacggc     1260

gagaacccta aggtggtgtt tatcgagatc tccgatcaga ggctgtctat cggctctccc     1320

agcaagatct acgactccct gggccagccc gtgttctacc aggcctcctt ttcttgggac     1380

acaatgatca agttcggcga tgtgctgacc gtgaatccac tggtggtgaa ctggagaaac     1440

aataccgtga tcagccggcc cggacagtcc cagtgtccta ggttcaacac atgcccagag     1500

atctgttggg agggcgtgta caatgacgcc ttcctgatcg atcggatcaa ctggatctcc     1560

gccggcgtgt ttctggactc taatcagacc gccgagaacc ccgtgttcac agtgtttaag     1620

gataatgaga tcctgtacag agcccagctg gcctctgagg acaccaacgc ccagaagacc     1680

atcacaaatt gcttcctgct gaagaacaag atctggtgta tcagcctggt ggagatctat     1740

gacaccggcg ataacgtgat ccggccaaag ctgtttgccg tgaagatccc cgagcagtgc     1800

aca                                                                   1803


<210>  6
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a consensus NiV-G immunogen

<400>  6

Pro Ala Glu Asn Lys Lys Val Arg Phe Glu Asn Thr Thr Ser Asp Lys 
1               5                   10                  15      


Gly Lys Ile Pro Ser Lys Val Ile Lys Ser Tyr Tyr Gly Thr Met Asp 
            20                  25                  30          


Ile Lys Lys Ile Asn Glu Gly Leu Leu Asp Ser Lys Ile Leu Ser Ala 
        35                  40                  45              


Phe Asn Thr Val Ile Ala Leu Leu Gly Ser Ile Val Ile Ile Val Met 
    50                  55                  60                  


Asn Ile Met Ile Ile Gln Asn Tyr Thr Arg Ser Thr Asp Asn Gln Ala 
65                  70                  75                  80  


Val Ile Lys Asp Ala Leu Gln Gly Ile Gln Gln Gln Ile Lys Gly Leu 
                85                  90                  95      


Ala Asp Lys Ile Gly Thr Glu Ile Gly Pro Lys Val Ser Leu Ile Asp 
            100                 105                 110         


Thr Ser Ser Thr Ile Thr Ile Pro Ala Asn Ile Gly Leu Leu Gly Ser 
        115                 120                 125             


Lys Ile Ser Gln Ser Thr Ala Ser Ile Asn Glu Asn Val Asn Glu Lys 
    130                 135                 140                 


Cys Lys Phe Thr Leu Pro Pro Leu Lys Ile His Glu Cys Asn Ile Ser 
145                 150                 155                 160 


Cys Pro Asn Pro Leu Pro Phe Arg Glu Tyr Arg Pro Gln Thr Glu Gly 
                165                 170                 175     


Val Ser Asn Leu Val Gly Leu Pro Asn Asn Ile Cys Leu Gln Lys Thr 
            180                 185                 190         


Ser Asn Gln Ile Leu Lys Pro Lys Leu Ile Ser Tyr Thr Leu Pro Val 
        195                 200                 205             


Val Gly Gln Ser Gly Thr Cys Ile Thr Asp Pro Leu Leu Ala Met Asp 
    210                 215                 220                 


Glu Gly Tyr Phe Ala Tyr Ser His Leu Glu Arg Ile Gly Ser Cys Ser 
225                 230                 235                 240 


Arg Gly Val Ser Lys Gln Arg Ile Ile Gly Val Gly Glu Val Leu Asp 
                245                 250                 255     


Arg Gly Asp Glu Val Pro Ser Leu Phe Met Thr Asn Val Trp Thr Pro 
            260                 265                 270         


Pro Asn Pro Asn Thr Val Tyr His Cys Ser Ala Val Tyr Asn Asn Glu 
        275                 280                 285             


Phe Tyr Tyr Val Leu Cys Ala Val Ser Thr Val Gly Asp Pro Ile Leu 
    290                 295                 300                 


Asn Ser Thr Tyr Trp Ser Gly Ser Leu Met Met Thr Arg Leu Ala Val 
305                 310                 315                 320 


Lys Pro Lys Ser Asn Gly Gly Gly Tyr Asn Gln His Gln Leu Ala Leu 
                325                 330                 335     


Arg Ser Ile Glu Lys Gly Arg Tyr Asp Lys Val Met Pro Tyr Gly Pro 
            340                 345                 350         


Ser Gly Ile Lys Gln Gly Asp Thr Leu Tyr Phe Pro Ala Val Gly Phe 
        355                 360                 365             


Leu Val Arg Thr Glu Phe Lys Tyr Asn Asp Ser Asn Cys Pro Ile Thr 
    370                 375                 380                 


Lys Cys Gln Tyr Ser Lys Pro Glu Asn Cys Arg Leu Ser Met Gly Ile 
385                 390                 395                 400 


Arg Pro Asn Ser His Tyr Ile Leu Arg Ser Gly Leu Leu Lys Tyr Asn 
                405                 410                 415     


Leu Ser Asp Gly Glu Asn Pro Lys Val Val Phe Ile Glu Ile Ser Asp 
            420                 425                 430         


Gln Arg Leu Ser Ile Gly Ser Pro Ser Lys Ile Tyr Asp Ser Leu Gly 
        435                 440                 445             


Gln Pro Val Phe Tyr Gln Ala Ser Phe Ser Trp Asp Thr Met Ile Lys 
    450                 455                 460                 


Phe Gly Asp Val Leu Thr Val Asn Pro Leu Val Val Asn Trp Arg Asn 
465                 470                 475                 480 


Asn Thr Val Ile Ser Arg Pro Gly Gln Ser Gln Cys Pro Arg Phe Asn 
                485                 490                 495     


Thr Cys Pro Glu Ile Cys Trp Glu Gly Val Tyr Asn Asp Ala Phe Leu 
            500                 505                 510         


Ile Asp Arg Ile Asn Trp Ile Ser Ala Gly Val Phe Leu Asp Ser Asn 
        515                 520                 525             


Gln Thr Ala Glu Asn Pro Val Phe Thr Val Phe Lys Asp Asn Glu Ile 
    530                 535                 540                 


Leu Tyr Arg Ala Gln Leu Ala Ser Glu Asp Thr Asn Ala Gln Lys Thr 
545                 550                 555                 560 


Ile Thr Asn Cys Phe Leu Leu Lys Asn Lys Ile Trp Cys Ile Ser Leu 
                565                 570                 575     


Val Glu Ile Tyr Asp Thr Gly Asp Asn Val Ile Arg Pro Lys Leu Phe 
            580                 585                 590         


Ala Val Lys Ile Pro Glu Gln Cys Thr 
        595                 600     


<210>  7
<211>  1863
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of a consensus NiV-G immunogen operably 
       linked to a sequence encoding an IgE leader and two stop codons

<400>  7
atggattgga catggattct gttcctggtg gcagcagcaa cccgcgtgca ctccccagcc       60

gagaataaga aggtgaggtt tgagaacacc acatctgaca agggcaagat cccctctaaa      120

gtgatcaaga gctactatgg caccatggac atcaagaaga tcaatgaggg cctgctggat      180

agcaagatcc tgtccgcctt caacacagtg atcgccctgc tgggctccat cgtgatcatc      240

gtgatgaata tcatgatcat ccagaactac accaggtcta cagacaatca ggccgtgatc      300

aaggacgctc tgcagggcat ccagcagcag atcaagggcc tggccgataa gatcggcaca      360

gagatcggcc ccaaggtgag cctgatcgac accagctcca ccatcacaat ccctgccaac      420

atcggcctgc tgggcagcaa gatctctcag agcaccgcct ccatcaacga gaatgtgaac      480

gagaagtgca agttcacact gccccctctg aagatccacg agtgcaatat ctcctgtcct      540

aacccactgc cctttaggga gtacagacca cagaccgagg gcgtgtctaa tctggtgggc      600

ctgcccaaca atatctgtct gcagaagacc agcaaccaga tcctgaagcc caagctgatc      660

tcctatacac tgcctgtggt gggccagtct ggcacctgca tcacagaccc tctgctggcc      720

atggatgagg gctacttcgc ctattctcac ctggagcgga tcggctcctg ttctcgcggc      780

gtgagcaagc agaggatcat cggagtggga gaggtgctgg acagaggcga tgaggtgcct      840

agcctgttca tgaccaacgt gtggacacca cccaatccaa acaccgtgta ccactgctcc      900

gccgtgtata acaatgagtt ttactacgtg ctgtgcgccg tgagcaccgt gggcgatcct      960

atcctgaact ccacatactg gagcggctcc ctgatgatga ccaggctggc agtgaagcca     1020

aagagcaatg gcggcggata taaccagcac cagctggccc tgagatccat cgagaagggc     1080

cggtacgata aagtgatgcc ttatggccca tccggcatca agcagggcga cacactgtac     1140

ttccccgccg tgggctttct ggtgaggacc gagttcaagt acaatgactc taactgccct     1200

atcacaaagt gtcagtattc taagccagag aattgccgcc tgagcatggg catcaggccc     1260

aactctcact acatcctgcg cagcggcctg ctgaagtata atctgagcga cggcgagaac     1320

cctaaggtgg tgtttatcga gatctccgat cagaggctgt ctatcggctc tcccagcaag     1380

atctacgact ccctgggcca gcccgtgttc taccaggcct ccttttcttg ggacacaatg     1440

atcaagttcg gcgatgtgct gaccgtgaat ccactggtgg tgaactggag aaacaatacc     1500

gtgatcagcc ggcccggaca gtcccagtgt cctaggttca acacatgccc agagatctgt     1560

tgggagggcg tgtacaatga cgccttcctg atcgatcgga tcaactggat ctccgccggc     1620

gtgtttctgg actctaatca gaccgccgag aaccccgtgt tcacagtgtt taaggataat     1680

gagatcctgt acagagccca gctggcctct gaggacacca acgcccagaa gaccatcaca     1740

aattgcttcc tgctgaagaa caagatctgg tgtatcagcc tggtggagat ctatgacacc     1800

ggcgataacg tgatccggcc aaagctgttt gccgtgaaga tccccgagca gtgcacatga     1860

taa                                                                   1863


<210>  8
<211>  619
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of a consensus NiV-G immunogen operably 
       linked to an IgE leader sequence

<400>  8

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Pro Ala Glu Asn Lys Lys Val Arg Phe Glu Asn Thr Thr Ser 
            20                  25                  30          


Asp Lys Gly Lys Ile Pro Ser Lys Val Ile Lys Ser Tyr Tyr Gly Thr 
        35                  40                  45              


Met Asp Ile Lys Lys Ile Asn Glu Gly Leu Leu Asp Ser Lys Ile Leu 
    50                  55                  60                  


Ser Ala Phe Asn Thr Val Ile Ala Leu Leu Gly Ser Ile Val Ile Ile 
65                  70                  75                  80  


Val Met Asn Ile Met Ile Ile Gln Asn Tyr Thr Arg Ser Thr Asp Asn 
                85                  90                  95      


Gln Ala Val Ile Lys Asp Ala Leu Gln Gly Ile Gln Gln Gln Ile Lys 
            100                 105                 110         


Gly Leu Ala Asp Lys Ile Gly Thr Glu Ile Gly Pro Lys Val Ser Leu 
        115                 120                 125             


Ile Asp Thr Ser Ser Thr Ile Thr Ile Pro Ala Asn Ile Gly Leu Leu 
    130                 135                 140                 


Gly Ser Lys Ile Ser Gln Ser Thr Ala Ser Ile Asn Glu Asn Val Asn 
145                 150                 155                 160 


Glu Lys Cys Lys Phe Thr Leu Pro Pro Leu Lys Ile His Glu Cys Asn 
                165                 170                 175     


Ile Ser Cys Pro Asn Pro Leu Pro Phe Arg Glu Tyr Arg Pro Gln Thr 
            180                 185                 190         


Glu Gly Val Ser Asn Leu Val Gly Leu Pro Asn Asn Ile Cys Leu Gln 
        195                 200                 205             


Lys Thr Ser Asn Gln Ile Leu Lys Pro Lys Leu Ile Ser Tyr Thr Leu 
    210                 215                 220                 


Pro Val Val Gly Gln Ser Gly Thr Cys Ile Thr Asp Pro Leu Leu Ala 
225                 230                 235                 240 


Met Asp Glu Gly Tyr Phe Ala Tyr Ser His Leu Glu Arg Ile Gly Ser 
                245                 250                 255     


Cys Ser Arg Gly Val Ser Lys Gln Arg Ile Ile Gly Val Gly Glu Val 
            260                 265                 270         


Leu Asp Arg Gly Asp Glu Val Pro Ser Leu Phe Met Thr Asn Val Trp 
        275                 280                 285             


Thr Pro Pro Asn Pro Asn Thr Val Tyr His Cys Ser Ala Val Tyr Asn 
    290                 295                 300                 


Asn Glu Phe Tyr Tyr Val Leu Cys Ala Val Ser Thr Val Gly Asp Pro 
305                 310                 315                 320 


Ile Leu Asn Ser Thr Tyr Trp Ser Gly Ser Leu Met Met Thr Arg Leu 
                325                 330                 335     


Ala Val Lys Pro Lys Ser Asn Gly Gly Gly Tyr Asn Gln His Gln Leu 
            340                 345                 350         


Ala Leu Arg Ser Ile Glu Lys Gly Arg Tyr Asp Lys Val Met Pro Tyr 
        355                 360                 365             


Gly Pro Ser Gly Ile Lys Gln Gly Asp Thr Leu Tyr Phe Pro Ala Val 
    370                 375                 380                 


Gly Phe Leu Val Arg Thr Glu Phe Lys Tyr Asn Asp Ser Asn Cys Pro 
385                 390                 395                 400 


Ile Thr Lys Cys Gln Tyr Ser Lys Pro Glu Asn Cys Arg Leu Ser Met 
                405                 410                 415     


Gly Ile Arg Pro Asn Ser His Tyr Ile Leu Arg Ser Gly Leu Leu Lys 
            420                 425                 430         


Tyr Asn Leu Ser Asp Gly Glu Asn Pro Lys Val Val Phe Ile Glu Ile 
        435                 440                 445             


Ser Asp Gln Arg Leu Ser Ile Gly Ser Pro Ser Lys Ile Tyr Asp Ser 
    450                 455                 460                 


Leu Gly Gln Pro Val Phe Tyr Gln Ala Ser Phe Ser Trp Asp Thr Met 
465                 470                 475                 480 


Ile Lys Phe Gly Asp Val Leu Thr Val Asn Pro Leu Val Val Asn Trp 
                485                 490                 495     


Arg Asn Asn Thr Val Ile Ser Arg Pro Gly Gln Ser Gln Cys Pro Arg 
            500                 505                 510         


Phe Asn Thr Cys Pro Glu Ile Cys Trp Glu Gly Val Tyr Asn Asp Ala 
        515                 520                 525             


Phe Leu Ile Asp Arg Ile Asn Trp Ile Ser Ala Gly Val Phe Leu Asp 
    530                 535                 540                 


Ser Asn Gln Thr Ala Glu Asn Pro Val Phe Thr Val Phe Lys Asp Asn 
545                 550                 555                 560 


Glu Ile Leu Tyr Arg Ala Gln Leu Ala Ser Glu Asp Thr Asn Ala Gln 
                565                 570                 575     


Lys Thr Ile Thr Asn Cys Phe Leu Leu Lys Asn Lys Ile Trp Cys Ile 
            580                 585                 590         


Ser Leu Val Glu Ile Tyr Asp Thr Gly Asp Asn Val Ile Arg Pro Lys 
        595                 600                 605             


Leu Phe Ala Val Lys Ile Pro Glu Gln Cys Thr 
    610                 615                 


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgE leader

<400>  9

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser 
        


