                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  MODIFIED SGRNAS

<130>  489.132643/02

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Target DNA

<400>  1
ccagccaagc gcacctaatt tcc                                               23


<210>  2
<211>  33
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide RNA

<400>  2
ggaaauuagg ugcgcuuggc guuuuagagc uag                                    33


<210>  3
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Insert for pCRISPR

<400>  3
ctagattata tgaacataac tcaatttgta aaaaagggta ttggggaatt cattac           56


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCRISPR. N is any nucleotide


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<400>  4
ataactcaat ttgtaaaaaa ngg                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSpotlight.  N is any nucleotide


<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<400>  5
ggacagtact ccgctcgagt ngg                                               23


<210>  6
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCRISPR-AsCpf1

<400>  6
tttatagtcc tgtcgggttt cgccac                                            26


<210>  7
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  cRNA

<400>  7
auaacucaau uuguaaaaaa uuuuagagcu augcuguuuu g                           41


<210>  8
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Truncated tracrRNA

<400>  8
aacagcauag caaguuaaaa uaaggcuagu ccguuauc                               38


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA blocker (Blocker 1)

<400>  9
gacaacttac tggataacgg actagcctt                                         29


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA antiblocker

<400>  10
aaggctagtc cgttatccag taagttgtc                                         29


<210>  11
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  cRNA

<400>  11
auaacucaau uuguaaaaaa uuuuagagcu augcuguuuu g                           41


<210>  12
<211>  89
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  12
guuggaacca uucaaaacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga       60

aaaaguggca ccgagucggu gcuuuuuuu                                         89


<210>  13
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 6x6 WH premir122

<400>  13
tattttgcct agcagtagct atttagtgtg ataatggcgt ttgacttgca tagtttagac       60

acaaacacca ttgtcacact ccacagctct gctaagg                                97


<210>  14
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 7x7 WH premir122

<400>  14
ttattttgcc tagcagtagc tatttagtgt gataatggcg tttgacttgc tatagtttag       60

acacaaacac cattgtcaca ctccacagct ctgctaagg                              99


<210>  15
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 8x8 WH premir122

<400>  15
cttattttgc ctagcagtag ctatttagtg tgataatggc gtttgacttg ctaatagttt       60

agacacaaac accattgtca cactccacag ctctgctaag g                          101


<210>  16
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 9x9 WH premir122

<400>  16
ccttattttg cctagcagta gctatttagt gtgataatgg cgtttgactt gctatatagt       60

ttagacacaa acaccattgt cacactccac agctctgcta agg                        103


<210>  17
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  premir122

<400>  17
ccuuagcaga gcuguggagu gugacaaugg uguuuguguc uaaacuauca aacgccauua       60

ucacacuaaa uagcuacugc uaggc                                             85


<210>  18
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  cRNA

<400>  18
auaacucaau uuguaaaaaa uuuuagagcu augcuguuuu g                           41


<210>  19
<211>  38
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Truncated tracrRNA

<400>  19
aacagcauag caaguuaaaa uaaggcuagu ccguuauc                               38


<210>  20
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 1.2

<400>  20
gauaacggac uaguccccua augggguuua uuuuaagggg                             40


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 5

<400>  21
gccttatttu aactgacaac tcaca                                             25


<210>  22
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 5.2

<400>  22
cuagccuuau uuuccccuaa ugggguacuu gcuauguuaa aa                          42


<210>  23
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blocker 6

<400>  23
ttttaacttg ctatgctgtt ttttgaaact t                                      31


<210>  24
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 6.2

<400>  24
uuauuuuaac uuguccccua auggggucua ugcuguuccc c                           41


<210>  25
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  cRNA

<400>  25
auaacucaau uuguaaaaaa uuuuagagcu augcuguuuu g                           41


<210>  26
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  26
tattttgcct agcagtagct atttagtgtg ataatggcgt ttgacttgca tagtttagac       60

acaaacacca ttgtcacact ccacagctct gctaagg                                97


<210>  27
<211>  101
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 8x8 WH premir122

<400>  27
cuuauuuugc cuagcaaaaa aaaaaaaaaa aaaaaaaggc guuugacuug cuaauaguuu       60

agacacaaac accauuguca cacuccacag cucugcuaag g                          101


<210>  28
<211>  103
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 9x9 WH premir122

<400>  28
ccuuauuuug ccuagcaaaa aaaaaaaaaa aaaaaaaagg cguuugacuu gcuauauagu       60

uuagacacaa acaccauugu cacacuccac agcucugcua agg                        103


<210>  29
<211>  105
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 10x10 WH premir122

<400>  29
gccuuauuuu gccuagcaaa aaaaaaaaaa aaaaaaaaag gcguuugacu ugcuaugaua       60

guuuagacac aaacaccauu gucacacucc acagcucugc uaagg                      105


<210>  30
<211>  107
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Blocker 11x11 WH premir122

<400>  30
agccuuauuu ugccuagcaa aaaaaaaaaa aaaaaaaaaa ggcguuugac uugcuaugca       60

uaguuuagac acaaacacca uugucacacu ccacagcucu gcuaagg                    107


<210>  31
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  premir122

<400>  31
ccuuagcaga gcuguggagu gugacaaugg uguuuguguc uaaacuauca aacgccauua       60

ucacacuaaa uagcuacugc uaggc                                             85


<210>  32
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  cRNA

<400>  32
auaacucaau uuguaaaaaa uuuuagagcu augcuguuuu g                           41


<210>  33
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  33
tattttgcct agcagtagct atttagtgtg ataatggcgt ttgacttgca tagtttagac       60

acaaacacca ttgtcacact ccacagctct gctaagg                                97


<210>  34
<211>  146
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA-3' Blocker A20 0StL

<400>  34
caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc       60

gagucggugc uaaaaaaaaa aaaaaaaaaa acuagccuua uuuuaaaaaa aaaaaaaaaa      120

aaaaaaaaaa aaaacuugcu augcug                                           146


<210>  35
<211>  176
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA-3' Blocker A20 3StL

<400>  35
caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc       60

gagucggugc uaaaaaaaaa aaaaaaaaaa acuagccuua uuuuaaggcg ccaaaaaggc      120

gccaacgccg caaaaagcgg cgaaccggcc aaaaaggccg gaaacuugcu augcug          176


<210>  36
<211>  115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  36
auaacucaau uuguaaaaaa guuuuagagc uagaaaccau ucaaaacagc auagcaaguu       60

aaaauaaggc uaguccguua ucaacuugaa aaaguggcac cgagucggug cuuuu           115


<210>  37
<211>  208
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 26 nt

<400>  37
auaacucaau uuguaaaaaa guuuuagagc uaugcuuuuu gaauggaaaa caaacaccac       60

uagccuuauu uuuugucaca cuccaaaaaa caaacaccau uguacuugcu augcugcaca      120

cuccaaaaac cauucaaaac agcauagcaa guuaaaauaa ggcuaguccg uuaucaacuu      180

gaaaaagugg caccgagucg gugcuuuu                                         208


<210>  38
<211>  261
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 3StL 26 nt

<400>  38
auaacucaau uuguaaaaaa guuuuagagc uaugcuuuuu gaauggaaaa caaacaccac       60

uagccuuauu uuuugucaca cuccaaaggc gccaaaaagg cgccaacgcc gcaaaaagcg      120

gcgaaccggc caaaaggccg gaacaaacac cauuguacuu gcuaugcugc acacuccaaa      180

aaccauucaa aacagcauag caaguuaaaa uaaggcuagu ccguuaucaa cuugaaaaag      240

uggcaccgag ucggugcuuu u                                                261


<210>  39
<211>  194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 12 nt

<400>  39
auaacucaau uuguaaaaaa guuuuagagc uagaaacaaa caccacuagc cuuauuuuuu       60

gucacacucc aaaaaacaaa caccauugua cuugcuaugc ugcacacucc aaaaaccauu      120

caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc      180

gagucggugc uuuu                                                        194


<210>  40
<211>  247
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 3StL 12 nt

<400>  40
auaacucaau uuguaaaaaa guuuuagagc uagaaacaaa caccacuagc cuuauuuuuu       60

gucacacucc aaaggcgcca aaaaggcgcc aacgccgcaa aaagcggcga accggccaaa      120

aggccggaac aaacaccauu guacuugcua ugcugcacac uccaaaaacc auucaaaaca      180

gcauagcaag uuaaaauaag gcuaguccgu uaucaacuug aaaaaguggc accgagucgg      240

ugcuuuu                                                                247


<210>  41
<211>  193
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 11 nt 5'

<400>  41
auaacucaau uuguaaaaaa uuuuagagcu agaaacaaac accacuagcc uuauuuuuug       60

ucacacucca aaaaacaaac accauuguac uugcuaugcu gcacacucca aaaaccauuc      120

aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa aguggcaccg      180

agucggugcu uuu                                                         193


<210>  42
<211>  193
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 11 nt 3'

<400>  42
auaacucaau uuguaaaaaa guuuuagagc ugaaacaaac accacuagcc uuauuuuuug       60

ucacacucca aaaaacaaac accauuguac uugcuaugcu gcacacucca aaaaccauuc      120

aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa aguggcaccg      180

agucggugcu uuu                                                         193


<210>  43
<211>  192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 10 nt

<400>  43
auaacucaau uuguaaaaaa uuuuagagcu gaaacaaaca ccacuagccu uauuuuuugu       60

cacacuccaa aaaacaaaca ccauuguacu ugcuaugcug cacacuccaa aaaccauuca      120

aaacagcaua gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa guggcaccga      180

gucggugcuu uu                                                          192


<210>  44
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  mRNA 122

<400>  44
uggaguguga caaugguguu ug                                                22


<210>  45
<211>  115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  45
auaacucaau uuguaaaaaa guuuuagagc uagaaaccau ucaaaacagc auagcaaguu       60

aaaauaaggc uaguccguua ucaacuugaa aaaguggcac cgagucggug cuuuu           115


<210>  46
<211>  194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 12 nt

<400>  46
auaacucaau uuguaaaaaa guuuuagagc uagaaacaaa caccacuagc cuuauuuuuu       60

gucacacucc aaaaaacaaa caccauugua cuugcuaugc ugcacacucc aaaaaccauu      120

caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc      180

gagucggugc uuuu                                                        194


<210>  47
<211>  192
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA 5' 0StL 10 nt

<400>  47
auaacucaau uuguaaaaaa uuuuagagcu gaaacaaaca ccacuagccu uauuuuuugu       60

cacacuccaa aaaacaaaca ccauuguacu ugcuaugcug cacacuccaa aaaccauuca      120

aaacagcaua gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa guggcaccga      180

gucggugcuu uu                                                          192


<210>  48
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miRNA 122

<400>  48
uggaguguga caaugguguu ug                                                22


<210>  49
<211>  115
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA

<400>  49
auaacucaau uuguaaaaaa guuuuagagc uagaaaccau ucaaaacagc auagcaaguu       60

aaaauaaggc uaguccguua ucaacuugaa aaaguggcac cgagucggug cuuuu           115


<210>  50
<211>  194
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m21 13x13

<400>  50
auaacucaau uuguaaaaaa guuuuagagc uagaaaucaa caucacuagc cuuauuuugu       60

cugauaagcu aaaaaaucaa caucagucua cuugcuaugc uggauaagcu aaaaaccauu      120

caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc      180

gagucggugc uuuu                                                        194


<210>  51
<211>  196
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m122 14x14

<400>  51
auaacucaau uuguaaaaaa guuuuagagc uagaaacaaa caccaacuag ccuuauuuuu       60

ugucacacuc caaaaaacaa acaccauugu acuugcuaug cugucacacu ccaaaaacca      120

uucaaaacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga aaaaguggca      180

ccgagucggu gcuuuu                                                      196


<210>  52
<211>  199
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m122 15x15

<400>  52
auaacucaau uuguaaaaaa guuuuagagc uagaaacaaa caccagacua gccuuauuuu       60

auugucacac uccaaaaaac aaacaccauu guacuugcua ugcuguucac acuccaaaaa      120

ccauucaaaa cagcauagca aguuaaaaua aggcuagucc guuaucaacu ugaaaaagug      180

gcaccgaguc ggugcuuuu                                                   199


<210>  53
<211>  196
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AND gate 14x14

<400>  53
auaacucaau uuguaaaaaa guuuuagagc uagaaaucaa caucaacuag ccuuauuuug       60

ucugauaagc uaaaaaacaa acaccauugu acuugcuaug cugucacacu ccaaaaacca      120

uucaaaacag cauagcaagu uaaaauaagg cuaguccguu aucaacuuga aaaaguggca      180

ccgagucggu gcuuuu                                                      196


<210>  54
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miRNA 21

<400>  54
uagcuuauca gacugauguu ga                                                22


<210>  55
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miRNA 122

<400>  55
uggaguguga caaugguguu ug                                                22


<210>  56
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgRNA Cpf1

<400>  56
aauuucuacu cuuguagauu aguccugucg gguuucgcca c                           41


<210>  57
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m21 8x8

<400>  57
ucaacaucac gaaacccguc ugauaagcua aaaaaucaac aucagucuga caggacgaua       60

agcuaaauuu cuacucuugu agauuagucc ugucggguuu cgccac                     106


<210>  58
<211>  108
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m21 9x9

<400>  58
ucaacaucag cgaaacccgu cugauaagcu aaaaaaucaa caucagucug acaggacuga       60

uaagcuaaau uucuacucuu guagauuagu ccugucgggu uucgccac                   108


<210>  59
<211>  110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Forward m21 10x10

<400>  59
ucaacaucag gcgaaacccg ucugauaagc uaaaaaauca acaucagucu gacaggacua       60

gauaagcuaa auuucuacuc uuguagauua guccugucgg guuucgccac                 110


<210>  60
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miRNA 21

<400>  60
uagcuuauca gacugauguu ga                                                22


