                         SEQUENCE LISTING

<110>  Translate Bio, Inc.
 
<120>  IMPROVED PROCESS OF PREPARING MRNA-LOADED LIPID NANOPARTICLES

<130>  MRT-2030WO

<150>  62/724,582
<151>  2018-08-29

<150>  62/725,765
<151>  2018-08-31

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1065
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  1
augcuguuca accuucggau cuugcugaac aacgcugcgu uccggaaugg ucacaacuuc       60

augguccgga acuucagaug cggccagccg cuccagaaca aggugcagcu caaggggagg      120

gaccuccuca cccugaaaaa cuucaccgga gaagagauca aguacaugcu guggcuguca      180

gccgaccuca aauuccggau caagcagaag ggcgaauacc uuccuuugcu gcagggaaag      240

ucccugggga ugaucuucga gaagcgcagc acucgcacua gacugucaac ugaaaccggc      300

uucgcgcugc ugggaggaca ccccugcuuc cugaccaccc aagauaucca ucugggugug      360

aacgaauccc ucaccgacac agcgcgggug cugucgucca uggcagacgc gguccucgcc      420

cgcguguaca agcagucuga ucuggacacu cuggccaagg aagccuccau uccuaucauu      480

aauggauugu ccgaccucua ccaucccauc cagauucugg ccgauuaucu gacucugcaa      540

gaacauuaca gcucccugaa ggggcuuacc cuuucgugga ucggcgacgg caacaacauu      600

cugcacagca uuaugaugag cgcugccaag uuuggaaugc accuccaagc agcgaccccg      660

aagggauacg agccagacgc cuccgugacg aagcuggcug agcaguacgc caaggagaac      720

ggcacuaagc ugcugcucac caacgacccu cucgaagccg cccacggugg caacgugcug      780

aucaccgaua ccuggaucuc caugggacag gaggaggaaa agaagaagcg ccugcaagca      840

uuucaggggu accaggugac uaugaaaacc gccaaggucg ccgccucgga cuggaccuuc      900

uugcacuguc ugcccagaaa gcccgaagag guggacgacg agguguucua cagcccgcgg      960

ucgcuggucu uuccggaggc cgaaaacagg aaguggacua ucauggccgu gauggugucc     1020

cugcugaccg auuacucccc gcagcugcag aaaccaaagu ucuga                     1065


<210>  2
<211>  1239
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  2
augagcagca agggcagcgu ggugcuggcc uacagcggcg gccuggacac cagcugcauc       60

cugguguggc ugaaggagca gggcuacgac gugaucgccu accuggccaa caucggccag      120

aaggaggacu ucgaggaggc ccgcaagaag gcccugaagc ugggcgccaa gaagguguuc      180

aucgaggacg ugagccgcga guucguggag gaguucaucu ggcccgccau ccagagcagc      240

gcccuguacg aggaccgcua ccugcugggc accagccugg cccgccccug caucgcccgc      300

aagcaggugg agaucgccca gcgcgagggc gccaaguacg ugagccacgg cgccaccggc      360

aagggcaacg accaggugcg cuucgagcug agcugcuaca gccuggcccc ccagaucaag      420

gugaucgccc ccuggcgcau gcccgaguuc uacaaccgcu ucaagggccg caacgaccug      480

auggaguacg ccaagcagca cggcaucccc auccccguga cccccaagaa ccccuggagc      540

auggacgaga accugaugca caucagcuac gaggccggca uccuggagaa ccccaagaac      600

caggcccccc ccggccugua caccaagacc caggaccccg ccaaggcccc caacaccccc      660

gacauccugg agaucgaguu caagaagggc gugcccguga aggugaccaa cgugaaggac      720

ggcaccaccc accagaccag ccuggagcug uucauguacc ugaacgaggu ggccggcaag      780

cacggcgugg gccgcaucga caucguggag aaccgcuuca ucggcaugaa gagccgcggc      840

aucuacgaga cccccgccgg caccauccug uaccacgccc accuggacau cgaggccuuc      900

accauggacc gcgaggugcg caagaucaag cagggccugg gccugaaguu cgccgagcug      960

guguacaccg gcuucuggca cagccccgag ugcgaguucg ugcgccacug caucgccaag     1020

agccaggagc gcguggaggg caaggugcag gugagcgugc ugaagggcca gguguacauc     1080

cugggccgcg agagcccccu gagccuguac aacgaggagc uggugagcau gaacgugcag     1140

ggcgacuacg agcccaccga cgccaccggc uucaucaaca ucaacagccu gcgccugaag     1200

gaguaccacc gccugcagag caaggugacc gccaaguga                            1239


<210>  3
<211>  4443
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  3
augcaacgcu cuccucuuga aaaggccucg guggugucca agcucuucuu cucguggacu       60

agacccaucc ugagaaaggg guacagacag cgcuuggagc uguccgauau cuaucaaauc      120

ccuuccgugg acuccgcgga caaccugucc gagaagcucg agagagaaug ggacagagaa      180

cucgccucaa agaagaaccc gaagcugauu aaugcgcuua ggcggugcuu uuucuggcgg      240

uucauguucu acggcaucuu ccucuaccug ggagagguca ccaaggccgu gcagccccug      300

uugcugggac ggauuauugc cuccuacgac cccgacaaca aggaagaaag aagcaucgcu      360

aucuacuugg gcaucggucu gugccugcuu uucaucgucc ggacccucuu guugcauccu      420

gcuauuuucg gccugcauca cauuggcaug cagaugagaa uugccauguu uucccugauc      480

uacaagaaaa cucugaagcu cucgagccgc gugcuugaca agauuuccau cggccagcuc      540

gugucccugc ucuccaacaa ucugaacaag uucgacgagg gccucgcccu ggcccacuuc      600

guguggaucg ccccucugca aguggcgcuu cugaugggcc ugaucuggga gcugcugcaa      660

gccucggcau ucugugggcu uggauuccug aucgugcugg cacuguucca ggccggacug      720

gggcggauga ugaugaagua cagggaccag agagccggaa agauuuccga acggcuggug      780

aucacuucgg aaaugaucga aaacauccag ucagugaagg ccuacugcug ggaagaggcc      840

auggaaaaga ugauugaaaa ccuccggcaa accgagcuga agcugacccg caaggccgcu      900

uacgugcgcu auuucaacuc guccgcuuuc uucuucuccg gguucuucgu gguguuucuc      960

uccgugcucc ccuacgcccu gauuaaggga aucauccuca ggaagaucuu caccaccauu     1020

uccuucugua ucgugcuccg cauggccgug acccggcagu ucccaugggc cgugcagacu     1080

ugguacgacu cccugggagc cauuaacaag auccaggacu uccuucaaaa gcaggaguac     1140

aagacccucg aguacaaccu gacuacuacc gaggucguga uggaaaacgu caccgccuuu     1200

ugggaggagg gauuuggcga acuguucgag aaggccaagc agaacaacaa caaccgcaag     1260

accucgaacg gugacgacuc ccucuucuuu ucaaacuuca gccugcucgg gacgcccgug     1320

cugaaggaca uuaacuucaa gaucgaaaga ggacagcucc uggcgguggc cggaucgacc     1380

ggagccggaa agacuucccu gcugauggug aucaugggag agcuugaacc uagcgaggga     1440

aagaucaagc acuccggccg caucagcuuc uguagccagu uuuccuggau caugcccgga     1500

accauuaagg aaaacaucau cuucggcgug uccuacgaug aauaccgcua ccgguccgug     1560

aucaaagccu gccagcugga agaggauauu ucaaaguucg cggagaaaga uaacaucgug     1620

cugggcgaag gggguauuac cuugucgggg ggccagcggg cuagaaucuc gcuggccaga     1680

gccguguaua aggacgccga ccuguaucuc cuggacuccc ccuucggaua ccuggacguc     1740

cugaccgaaa aggagaucuu cgaaucgugc gugugcaagc ugauggcuaa caagacucgc     1800

auccucguga ccuccaaaau ggagcaccug aagaaggcag acaagauucu gauucugcau     1860

gagggguccu ccuacuuuua cggcaccuuc ucggaguugc agaacuugca gcccgacuuc     1920

ucaucgaagc ugauggguug cgacagcuuc gaccaguucu ccgccgaaag aaggaacucg     1980

auccugacgg aaaccuugca ccgcuucucu uuggaaggcg acgccccugu gucauggacc     2040

gagacuaaga agcagagcuu caagcagacc ggggaauucg gcgaaaagag gaagaacagc     2100

aucuugaacc ccauuaacuc cauccgcaag uucucaaucg ugcaaaagac gccacugcag     2160

augaacggca uugaggagga cuccgacgaa ccccuugaga ggcgccuguc ccuggugccg     2220

gacagcgagc agggagaagc cauccugccu cggauuuccg ugaucuccac ugguccgacg     2280

cuccaagccc ggcggcggca guccgugcug aaccugauga cccacagcgu gaaccagggc     2340

caaaacauuc accgcaagac uaccgcaucc acccggaaag ugucccuggc accucaagcg     2400

aaucuuaccg agcucgacau cuacucccgg agacugucgc aggaaaccgg gcucgaaauu     2460

uccgaagaaa ucaacgagga ggaucugaaa gagugcuucu ucgacgauau ggagucgaua     2520

cccgccguga cgacuuggaa cacuuaucug cgguacauca cugugcacaa gucauugauc     2580

uucgugcuga uuuggugccu ggugauuuuc cuggccgagg ucgcggccuc acugguggug     2640

cucuggcugu ugggaaacac gccucugcaa gacaagggaa acuccacgca cucgagaaac     2700

aacagcuaug ccgugauuau cacuuccacc uccucuuauu acguguucua caucuacguc     2760

ggaguggcgg auacccugcu cgcgaugggu uucuucagag gacugccgcu gguccacacc     2820

uugaucaccg ucagcaagau ucuucaccac aagauguugc auagcgugcu gcaggccccc     2880

auguccaccc ucaacacucu gaaggccgga ggcauucuga acagauucuc caaggacauc     2940

gcuauccugg acgaucuccu gccgcuuacc aucuuugacu ucauccagcu gcugcugauc     3000

gugauuggag caaucgcagu gguggcggug cugcagccuu acauuuucgu ggccacugug     3060

ccggucauug uggcguucau caugcugcgg gccuacuucc uccaaaccag ccagcagcug     3120

aagcaacugg aauccgaggg acgauccccc aucuucacuc accuugugac gucguugaag     3180

ggacugugga cccuccgggc uuucggacgg cagcccuacu ucgaaacccu cuuccacaag     3240

gcccugaacc uccacaccgc caauugguuc cuguaccugu ccacccugcg gugguuccag     3300

augcgcaucg agaugauuuu cgucaucuuc uucaucgcgg ucacauucau cagcauccug     3360

acuaccggag agggagaggg acgggucgga auaauccuga cccucgccau gaacauuaug     3420

agcacccugc agugggcagu gaacagcucg aucgacgugg acagccugau gcgaagcguc     3480

agccgcgugu ucaaguucau cgacaugccu acugagggaa aacccacuaa guccacuaag     3540

cccuacaaaa auggccagcu gagcaagguc augaucaucg aaaacuccca cgugaagaag     3600

gacgauauuu ggcccuccgg aggucaaaug accgugaagg accugaccgc aaaguacacc     3660

gagggaggaa acgccauucu cgaaaacauc agcuucucca uuucgccggg acagcggguc     3720

ggccuucucg ggcggaccgg uuccgggaag ucaacucugc ugucggcuuu ccuccggcug     3780

cugaauaccg agggggaaau ccaaauugac ggcgugucuu gggauuccau uacucugcag     3840

caguggcgga aggccuucgg cgugaucccc cagaaggugu ucaucuucuc ggguaccuuc     3900

cggaagaacc uggauccuua cgagcagugg agcgaccaag aaaucuggaa ggucgccgac     3960

gaggucggcc ugcgcuccgu gauugaacaa uuuccuggaa agcuggacuu cgugcucguc     4020

gacgggggau guguccuguc gcacggacau aagcagcuca ugugccucgc acgguccgug     4080

cucuccaagg ccaagauucu gcugcuggac gaaccuucgg cccaccugga uccggucacc     4140

uaccagauca ucaggaggac ccugaagcag gccuuugccg auugcaccgu gauucucugc     4200

gagcaccgca ucgaggccau gcuggagugc cagcaguucc uggucaucga ggagaacaag     4260

guccgccaau acgacuccau ucaaaagcuc cucaacgagc ggucgcuguu cagacaagcu     4320

auuucaccgu ccgauagagu gaagcucuuc ccgcaucgga acagcucaaa gugcaaaucg     4380

aagccgcaga ucgcagccuu gaaggaagag acugaggaag aggugcagga cacccggcuu     4440

uaa                                                                   4443


<210>  4
<211>  4443
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  4
augcagcggu ccccgcucga aaaggccagu gucgugucca aacucuucuu cucauggacu       60

cggccuaucc uuagaaaggg guaucggcag aggcuugagu ugucugacau cuaccagauc      120

cccucgguag auucggcgga uaaccucucg gagaagcucg aacgggaaug ggaccgcgaa      180

cucgcgucua agaaaaaccc gaagcucauc aacgcacuga gaaggugcuu cuucuggcgg      240

uucauguucu acgguaucuu cuuguaucuc ggggagguca caaaagcagu ccaaccccug      300

uuguuggguc gcauuaucgc cucguacgac cccgauaaca aagaagaacg gagcaucgcg      360

aucuaccucg ggaucggacu guguuugcuu uucaucguca gaacacuuuu guugcaucca      420

gcaaucuucg gccuccauca caucgguaug cagaugcgaa ucgcuauguu uagcuugauc      480

uacaaaaaga cacugaaacu cucgucgcgg guguuggaua agauuuccau cggucaguug      540

gugucccugc uuaguaauaa ccucaacaaa uucgaugagg gacuggcgcu ggcacauuuc      600

guguggauug ccccguugca agucgcccuu uugaugggcc uuauuuggga gcuguugcag      660

gcaucugccu uuuguggccu gggauuucug auuguguugg cauuguuuca ggcugggcuu      720

gggcggauga ugaugaagua ucgcgaccag agagcgggua aaaucucgga aagacucguc      780

aucacuucgg aaaugaucga aaacauccag ucggucaaag ccuauugcug ggaagaagcu      840

auggagaaga ugauugaaaa ccuccgccaa acugagcuga aacugacccg caaggcggcg      900

uauguccggu auuucaauuc gucagcguuc uucuuuuccg gguucuucgu ugucuuucuc      960

ucgguuuugc cuuaugccuu gauuaagggg auuauccucc gcaagauuuu caccacgauu     1020

ucguucugca uuguauugcg cauggcagug acacggcaau uuccgugggc cgugcagaca     1080

ugguaugacu cgcuuggagc gaucaacaaa auccaagacu ucuugcaaaa gcaagaguac     1140

aagacccugg aguacaaucu uacuacuacg gagguaguaa uggagaaugu gacggcuuuu     1200

ugggaagagg guuuuggaga acuguuugag aaagcaaagc agaauaacaa caaccgcaag     1260

accucaaaug gggacgauuc ccuguuuuuc ucgaacuucu cccugcucgg aacacccgug     1320

uugaaggaca ucaauuucaa gauugagagg ggacagcuuc ucgcgguagc gggaagcacu     1380

ggugcgggaa aaacuagccu cuugauggug auuauggggg agcuugagcc cagcgagggg     1440

aagauuaaac acuccgggcg uaucucauuc uguagccagu uuucauggau caugcccgga     1500

accauuaaag agaacaucau uuucggagua uccuaugaug aguaccgaua cagaucgguc     1560

auuaaggcgu gccaguugga agaggacauu ucuaaguucg ccgagaagga uaacaucguc     1620

uugggagaag gggguauuac auugucggga gggcagcgag cgcggaucag ccucgcgaga     1680

gcgguauaca aagaugcaga uuuguaucug cuugauucac cguuuggaua ccucgacgua     1740

uugacagaaa aagaaaucuu cgagucgugc guguguaaac uuauggcuaa uaagacgaga     1800

auccugguga caucaaaaau ggaacaccuu aagaaggcgg acaagauccu gauccuccac     1860

gaaggaucgu ccuacuuuua cggcacuuuc ucagaguugc aaaacuugca gccggacuuc     1920

ucaagcaaac ucauggggug ugacucauuc gaccaguuca gcgcggaacg gcggaacucg     1980

aucuugacgg aaacgcugca ccgauucucg cuugagggug augccccggu aucguggacc     2040

gagacaaaga agcagucguu uaagcagaca ggagaauuug gugagaaaag aaagaacagu     2100

aucuugaauc cuauuaacuc aauucgcaag uucucaaucg uccagaaaac uccacugcag     2160

augaauggaa uugaagagga uucggacgaa ccccuggagc gcaggcuuag ccucgugccg     2220

gauucagagc aaggggaggc cauucuuccc cggauuucgg ugauuucaac cggaccuaca     2280

cuucaggcga ggcgaaggca auccgugcuc aaccucauga cgcauucggu aaaccagggg     2340

caaaacauuc accgcaaaac gacggccuca acgagaaaag ugucacuugc accccaggcg     2400

aauuugacug aacucgacau cuacagccgu aggcuuucgc aagaaaccgg acuugagauc     2460

agcgaagaaa ucaaugaaga agauuugaaa gaguguuucu uugaugacau ggaaucaauc     2520

ccagcgguga caacguggaa cacauacuug cguuacauca cggugcacaa guccuugauu     2580

uucguccuca ucuggugucu cgugaucuuu cucgcugagg ucgcagcguc acuugugguc     2640

cucuggcugc uugguaauac gcccuugcaa gacaaaggca auucuacaca cucaagaaac     2700

aauuccuaug ccgugauuau cacuucuaca agcucguauu acguguuuua caucuacgua     2760

ggaguggccg acacucugcu cgcgaugggu uucuuccgag gacucccacu cguucacacg     2820

cuuaucacug ucuccaagau ucuccaccau aagaugcuuc auagcguacu gcaggcuccc     2880

auguccaccu ugaauacgcu caaggcggga gguauuuuga aucgcuucuc aaaagauauu     2940

gcaauuuugg augaccuucu gccccugacg aucuucgacu ucauccaguu guugcugauc     3000

gugauugggg cuauugcagu agucgcuguc cuccagccuu acauuuuugu cgcgaccguu     3060

ccggugaucg uggcguuuau caugcugcgg gccuauuucu ugcagacguc acagcagcuu     3120

aagcaacugg agucugaagg gaggucgccu aucuuuacgc aucuugugac caguuugaag     3180

ggauugugga cguugcgcgc cuuuggcagg cagcccuacu uugaaacacu guuccacaaa     3240

gcgcugaauc uccauacggc aaauugguuu uuguauuuga guacccuccg augguuucag     3300

augcgcauug agaugauuuu ugugaucuuc uuuaucgcgg ugacuuuuau cuccaucuug     3360

accacgggag agggcgaggg acgggucggu auuauccuga cacucgccau gaacauuaug     3420

agcacuuugc agugggcagu gaacagcucg auugaugugg auagccugau gagguccguu     3480

ucgagggucu uuaaguucau cgacaugccg acggagggaa agcccacaaa aaguacgaaa     3540

cccuauaaga augggcaauu gaguaaggua augaucaucg agaacaguca cgugaagaag     3600

gaugacaucu ggccuagcgg gggucagaug accgugaagg accugacggc aaaauacacc     3660

gagggaggga acgcaauccu ugaaaacauc ucguucagca uuagccccgg ucagcgugug     3720

ggguugcucg ggaggaccgg gucaggaaaa ucgacguugc ugucggccuu cuugagacuu     3780

cugaauacag agggugagau ccagaucgac ggcguuucgu gggauagcau caccuugcag     3840

caguggcgga aagcguuugg aguaaucccc caaaaggucu uuaucuuuag cggaaccuuc     3900

cgaaagaauc ucgauccuua ugaacagugg ucagaucaag agauuuggaa agucgcggac     3960

gagguuggcc uucggagugu aaucgagcag uuuccgggaa aacucgacuu uguccuugua     4020

gaugggggau gcguccuguc gcaugggcac aagcagcuca ugugccuggc gcgauccguc     4080

cucucuaaag cgaaaauucu ucucuuggau gaaccuucgg cccaucugga cccgguaacg     4140

uaucagauca ucagaaggac acuuaagcag gcguuugccg acugcacggu gauucucugu     4200

gagcaucgua ucgaggccau gcucgaaugc cagcaauuuc uugucaucga agagaauaag     4260

guccgccagu acgacuccau ccagaagcug cuuaaugaga gaucauuguu ccggcaggcg     4320

auuucaccau ccgauagggu gaaacuuuuu ccacacagaa auucgucgaa gugcaagucc     4380

aaaccgcaga ucgcggccuu gaaagaagag acugaagaag aaguucaaga cacgcgucuu     4440

uaa                                                                   4443


<210>  5
<211>  1359
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide

<400>  5
augagcaccg ccgugcugga gaaccccggc cugggccgca agcugagcga cuucggccag       60

gagaccagcu acaucgagga caacugcaac cagaacggcg ccaucagccu gaucuucagc      120

cugaaggagg aggugggcgc ccuggccaag gugcugcgcc uguucgagga gaacgacgug      180

aaccugaccc acaucgagag ccgccccagc cgccugaaga aggacgagua cgaguucuuc      240

acccaccugg acaagcgcag ccugcccgcc cugaccaaca ucaucaagau ccugcgccac      300

gacaucggcg ccaccgugca cgagcugagc cgcgacaaga agaaggacac cgugcccugg      360

uucccccgca ccauccagga gcuggaccgc uucgccaacc agauccugag cuacggcgcc      420

gagcuggacg ccgaccaccc cggcuucaag gaccccgugu accgcgcccg ccgcaagcag      480

uucgccgaca ucgccuacaa cuaccgccac ggccagccca ucccccgcgu ggaguacaug      540

gaggaggaga agaagaccug gggcaccgug uucaagaccc ugaagagccu guacaagacc      600

cacgccugcu acgaguacaa ccacaucuuc ccccugcugg agaaguacug cggcuuccac      660

gaggacaaca ucccccagcu ggaggacgug agccaguucc ugcagaccug caccggcuuc      720

cgccugcgcc ccguggccgg ccugcugagc agccgcgacu uccugggcgg ccuggccuuc      780

cgcguguucc acugcaccca guacauccgc cacggcagca agcccaugua cacccccgag      840

cccgacaucu gccacgagcu gcugggccac gugccccugu ucagcgaccg cagcuucgcc      900

caguucagcc aggagaucgg ccuggccagc cugggcgccc ccgacgagua caucgagaag      960

cuggccacca ucuacugguu caccguggag uucggccugu gcaagcaggg cgacagcauc     1020

aaggccuacg gcgccggccu gcugagcagc uucggcgagc ugcaguacug ccugagcgag     1080

aagcccaagc ugcugccccu ggagcuggag aagaccgcca uccagaacua caccgugacc     1140

gaguuccagc cccuguacua cguggccgag agcuucaacg acgccaagga gaaggugcgc     1200

aacuucgccg ccaccauccc ccgccccuuc agcgugcgcu acgaccccua cacccagcgc     1260

aucgaggugc uggacaacac ccagcagcug aagauccugg ccgacagcau caacagcgag     1320

aucggcaucc ugugcagcgc ccugcagaag aucaaguaa                            1359


