               SEQUENCE LISTING

<110> FrieslandCampina Nederland B.V.

<120> Bifidogenic GOS compositions and methods for providing the same

<130> P1604EP00

<160> 2

<170> BiSSAP 1.3.6

<210> 1
<211> 1128
<212> PRT
<213> Lactobacillus delbrueckii 


<400> 1
Met Ser Asn Lys Leu Val Lys Glu Lys Arg Val Asp Gln Ala Asp Leu 
1               5                   10                  15      
Ala Trp Leu Thr Asp Pro Glu Val Tyr Glu Val Asn Thr Ile Pro Pro 
            20                  25                  30          
His Ser Asp His Glu Ser Phe Gln Ser Gln Glu Glu Leu Glu Glu Gly 
        35                  40                  45              
Lys Ser Ser Leu Val Gln Ser Leu Asp Gly Asp Trp Leu Ile Asp Tyr 
    50                  55                  60                  
Ala Glu Asn Gly Gln Gly Pro Val Asn Phe Tyr Ala Glu Asp Phe Asp 
65                  70                  75                  80  
Asp Ser Asn Phe Lys Ser Val Lys Val Pro Gly Asn Leu Glu Leu Gln 
                85                  90                  95      
Gly Phe Gly Gln Pro Gln Tyr Val Asn Val Gln Tyr Pro Trp Asp Gly 
            100                 105                 110         
Ser Glu Glu Ile Phe Pro Pro Gln Ile Pro Ser Lys Asn Pro Leu Ala 
        115                 120                 125             
Ser Tyr Val Arg Tyr Phe Asp Leu Asp Glu Ala Phe Trp Asp Lys Glu 
    130                 135                 140                 
Val Ser Leu Lys Phe Asp Gly Ala Ala Thr Ala Ile Tyr Val Trp Leu 
145                 150                 155                 160 
Asn Gly His Phe Val Gly Tyr Gly Glu Asp Ser Phe Thr Pro Ser Glu 
                165                 170                 175     
Phe Met Val Thr Lys Phe Leu Lys Lys Glu Asn Asn Arg Leu Ala Val 
            180                 185                 190         
Ala Leu Tyr Lys Tyr Ser Ser Ala Ser Trp Leu Glu Asp Gln Asp Phe 
        195                 200                 205             
Trp Arg Met Ser Gly Leu Phe Arg Ser Val Thr Leu Gln Ala Lys Pro 
    210                 215                 220                 
Arg Leu His Leu Glu Asp Leu Lys Leu Thr Ala Ser Leu Thr Asp Asn 
225                 230                 235                 240 
Tyr Gln Lys Gly Lys Leu Glu Val Glu Ala Asn Ile Ala Tyr Arg Leu 
                245                 250                 255     
Pro Asn Ala Ser Phe Lys Leu Glu Val Arg Asp Ser Glu Gly Asp Leu 
            260                 265                 270         
Val Ala Glu Lys Leu Gly Pro Ile Arg Ser Glu Gln Leu Glu Phe Thr 
        275                 280                 285             
Leu Ala Asp Leu Pro Val Ala Ala Trp Ser Ala Glu Lys Pro Asn Leu 
    290                 295                 300                 
Tyr Gln Val Arg Leu Tyr Leu Tyr Gln Ala Gly Ser Leu Leu Glu Val 
305                 310                 315                 320 
Ser Arg Gln Glu Val Gly Phe Arg Asn Phe Glu Leu Lys Asp Gly Ile 
                325                 330                 335     
Met Tyr Leu Asn Gly Gln Arg Ile Val Phe Lys Gly Ala Asn Arg His 
            340                 345                 350         
Glu Phe Asp Ser Lys Leu Gly Arg Ala Ile Thr Glu Glu Asp Met Ile 
        355                 360                 365             
Trp Asp Ile Lys Thr Met Lys Arg Ser Asn Ile Asn Ala Val Arg Cys 
    370                 375                 380                 
Ser His Tyr Pro Asn Gln Ser Leu Phe Tyr Arg Leu Cys Asp Lys Tyr 
385                 390                 395                 400 
Gly Leu Tyr Val Ile Asp Glu Ala Asn Leu Glu Ser His Gly Thr Trp 
                405                 410                 415     
Glu Lys Val Gly Gly His Glu Asp Pro Ser Phe Asn Val Pro Gly Asp 
            420                 425                 430         
Asp Gln His Trp Leu Gly Ala Ser Leu Ser Arg Val Lys Asn Met Met 
        435                 440                 445             
Ala Arg Asp Lys Asn His Ala Ser Ile Leu Ile Trp Ser Leu Gly Asn 
    450                 455                 460                 
Glu Ser Tyr Ala Gly Thr Val Phe Ala Gln Met Ala Asp Tyr Val Arg 
465                 470                 475                 480 
Lys Ala Asp Pro Thr Arg Val Gln His Tyr Glu Gly Val Thr His Asn 
                485                 490                 495     
Arg Lys Phe Asp Asp Ala Thr Gln Ile Glu Ser Arg Met Tyr Ala Pro 
            500                 505                 510         
Ala Lys Val Ile Glu Glu Tyr Leu Thr Asn Lys Pro Ala Lys Pro Phe 
        515                 520                 525             
Ile Ser Val Glu Tyr Ala His Ala Met Gly Asn Ser Lys Ala Asp Pro 
    530                 535                 540                 
Thr Arg Val Gln His Tyr Glu Gly Val Thr His Asn Arg Lys Phe Asp 
545                 550                 555                 560 
Asp Ala Thr Gln Ile Glu Ser Arg Met Tyr Ala Pro Ala Lys Val Ile 
                565                 570                 575     
Glu Glu Tyr Leu Thr Asn Lys Pro Ala Lys Pro Phe Ile Ser Val Glu 
            580                 585                 590         
Tyr Ala His Ala Met Gly Asn Ser Lys Ala Asp Pro Thr Arg Val Gln 
        595                 600                 605             
His Tyr Glu Gly Val Thr His Asn Arg Lys Phe Asp Asp Ala Thr Gln 
    610                 615                 620                 
Ile Glu Ser Arg Met Tyr Ala Pro Ala Lys Val Ile Glu Glu Tyr Leu 
625                 630                 635                 640 
Thr Asn Lys Pro Ala Lys Pro Phe Ile Ser Val Glu Tyr Ala His Ala 
                645                 650                 655     
Met Gly Asn Ser Val Gly Asp Leu Ala Ala Tyr Thr Ala Leu Glu Lys 
            660                 665                 670         
Tyr Pro His Tyr Gln Gly Gly Phe Ile Trp Asp Trp Ile Asp Gln Gly 
        675                 680                 685             
Leu Glu Lys Asp Gly His Leu Leu Tyr Gly Gly Asp Phe Asp Asp Arg 
    690                 695                 700                 
Pro Thr Asp Tyr Glu Phe Cys Gly Asn Gly Leu Val Phe Ala Asp Arg 
705                 710                 715                 720 
Thr Glu Ser Pro Lys Leu Ala Asn Val Lys Ala Leu Tyr Ala Asn Leu 
                725                 730                 735     
Lys Leu Glu Val Lys Asp Gly Gln Leu Phe Leu Lys Asn Asp Asn Leu 
            740                 745                 750         
Phe Thr Asn Ser Ser Ser Tyr Tyr Phe Leu Thr Ser Leu Leu Val Asp 
        755                 760                 765             
Gly Lys Leu Thr Tyr Gln Ser Arg Pro Leu Thr Phe Gly Leu Glu Pro 
    770                 775                 780                 
Gly Glu Ser Gly Thr Phe Ala Leu Pro Trp Pro Glu Val Ala Asp Glu 
785                 790                 795                 800 
Lys Gly Glu Val Val Tyr Arg Val Thr Ala His Leu Lys Glu Asp Leu 
                805                 810                 815     
Pro Trp Ala Asp Glu Gly Phe Thr Val Ala Glu Ala Glu Glu Val Ala 
            820                 825                 830         
Gln Lys Leu Pro Glu Phe Lys Pro Glu Gly Arg Pro Asp Leu Val Asp 
        835                 840                 845             
Ser Asp Tyr Asn Leu Gly Leu Lys Gly Asn Asn Phe Gln Ile Leu Phe 
    850                 855                 860                 
Ser Lys Val Lys Gly Trp Pro Val Ser Leu Lys Tyr Ala Gly Arg Glu 
865                 870                 875                 880 
Tyr Leu Lys Arg Leu Pro Glu Phe Thr Phe Trp Arg Ala Leu Thr Asp 
                885                 890                 895     
Asn Asp Arg Gly Ala Gly Tyr Gly Tyr Asp Leu Ala Arg Trp Glu Asn 
            900                 905                 910         
Ala Gly Lys Tyr Ala Arg Leu Lys Asp Ile Ser Cys Glu Val Lys Glu 
        915                 920                 925             
Asp Ser Val Leu Val Lys Thr Ala Phe Thr Leu Pro Val Ala Leu Lys 
    930                 935                 940                 
Gly Asp Leu Thr Val Thr Tyr Glu Val Asp Gly Arg Gly Lys Ile Ala 
945                 950                 955                 960 
Val Thr Ala Asp Phe Pro Gly Ala Glu Glu Ala Gly Leu Leu Pro Ala 
                965                 970                 975     
Phe Gly Leu Asn Leu Ala Leu Pro Lys Glu Leu Thr Asp Tyr Arg Tyr 
            980                 985                 990         
Tyr Gly Leu Gly Pro Asn Glu Ser Tyr Pro Asp Arg Leu Glu Gly Asn 
        995                 1000                1005            
Tyr Leu Gly Ile Tyr Gln Gly Ala Val Lys Lys Asn Phe Ser Pro Tyr 
    1010                1015                1020                
Leu Arg Pro Gln Glu Thr Gly Asn Arg Ser Lys Val Arg Trp Tyr Gln 
1025                1030                1035                1040
Leu Phe Asp Glu Lys Gly Gly Leu Glu Phe Thr Ala Asn Gly Ala Asp 
                1045                1050                1055    
Leu Asn Leu Ser Ala Leu Pro Tyr Ser Ala Ala Gln Ile Glu Ala Ala 
            1060                1065                1070        
Asp His Ala Phe Glu Leu Thr Asn Asn Tyr Thr Trp Val Arg Ala Leu 
        1075                1080                1085            
Ser Ala Gln Met Gly Val Gly Gly Asp Asp Ser Trp Gly Gln Lys Val 
    1090                1095                1100                
His Pro Glu Phe Cys Leu Asp Ala Gln Lys Ala Arg Gln Leu Arg Leu 
1105                1110                1115                1120
Val Ile Gln Pro Leu Leu Leu Lys 
                1125            

<210> 2
<211> 3027
<212> DNA
<213> Lactobacillus delbrueckii 


<400> 2
ttattttagt aaaaggggct gaatcaccag gcggagctgg cgggcttttt gagcatccag      60

gcagaattcc gggtggacct tctgccccca ggagtcatcc ccgccgaccc ccatctgggc     120

gcttaaggct ctaacccaag tgtaattgtt agtcagttca aaagcgtggt ccgctgcttc     180

aatttgggcg gcagaatatg gcaaagcaga caagttcaag tctgccccat tggccgtaaa     240

ttccaagccg cccttttcat caaagagctg gtaccagcga accttgctcc ggttgcccgt     300

ttcctgcgga cgcaggtatg ggctaaagtt cttttttacc gctccctggt agatgcccag     360

gtaattacct tccaagcggt ctgggtagct ctcattaggt cccagaccat agtagcggta     420

atcggtcagt tcttttggca gggccaggtt caagccaaag gctggcaaga ggccggcttc     480

ttccgcgcct gggaagtcag ctgttacagc aatcttgccc cgtccatcga cttcataggt     540

cacggttaaa tcacccttta aggcgacagg caacgtaaag gcagtcttga ccaaaacgga     600

gtcttccttg acctcgcagc tgatgtcttt caagcgggca tacttgccgg cattttccca     660

ccgggccaga tcatagccgt aaccagctcc ccggtcgttg tccgtcaggg cccgccagaa     720

ggtaaattcc ggcagccgct tcaagtattc cctaccggca tacttgaggg aaaccggcca     780

gcccttgacc ttggagaaga gaatttggaa gttatttcct ttcaggccta ggttgtagtc     840

ggaatcaact aaatctggcc gcccttccgg cttaaattcc ggcagctttt gagctacttc     900

ttctgcttca gccacagtga agccctcatc cgcccaaggc aagtcttctt ttaagtgggc     960

cgttacccgg tagacgacct ctcctttttc atcagcgact tccggccaag gcagggcaaa    1020

ggtcccggat tcgccaggct ccaggccaaa ggtcagaggc cggctctggt aggtcaactt    1080

gccatcgacc aaaagactag tcaagaagta gtaagatgag ctgttggtaa ataaattgtc    1140

gtttttgagg aagagctgcc catcttttac ttctaactta aggttggcgt aaagggcctt    1200

gacattagcc agtttcggcg attcagtccg gtcagcaaag accaggccgt tcccgcagaa    1260

ttcatagtcg gttggccggt catcgaagtc gcccccataa agcaggtgcc cgtctttttc    1320

cagtccttgg tcaatccagt cccagatgaa gccgccctgg tagtgggggt atttttccag    1380

ggccgtgtag gcggccaggt caccgacgga gttgcccatg gcgtgagcgt attcaactga    1440

gataaatggc ttggctggtt tattggtcaa gtattcttca attaccttgg ccggagcata    1500

catccggctt tcaatctggg tggcgtcgtc aaacttccgg ttgtgggtca ccccttcata    1560

gtgctgaacc cgggtcggat cagccttccg gacgtaatca gccatttggg caaagacagt    1620

gccggcgtaa gactcattgc ctaaagacca gatcaggatt gaagcatggt tcttgtcccg    1680

agccatcatg ttcttcaccc gggataagct ggctcccagc caatgctggt catcgcctgg    1740

aacattgaag ctaggatctt cgtgcccccc cactttttcc caggtgccgt ggctttccag    1800

gttagcttca tcaatgacgt aaaggccgta cttgtcacag agccggtaaa agagggactg    1860

gttcgggtag tgagagcagc ggacagcatt gatgttgctt cgcttcatgg tcttgatatc    1920

ccagatcata tcctcttctg tgatagcccg gcccaactta ctgtcaaatt cgtgccggtt    1980

ggcccccttg aagacgatcc gctggccgtt aaggtacata atcccgtctt ttagttcaaa    2040

gttgcggaaa cccacttcct gccggctaac ctctaagagg ctgcctgcct ggtataaata    2100

caggcggacc tggtaaaggt taggcttttc cgcgctccag gcagctactg gcaaatcagc    2160

cagagtgaat tccagctgct cgcttctgat tgggcccagc ttttcagcaa ccaagtcacc    2220

ttcactatcc cgcacttcca gcttaaagct ggcatttggc aagcggtagg caatattggc    2280

ttcgacttcc agctttcctt tttggtagtt atcggtcaag ctggccgtaa gcttaaggtc    2340

ctccaagtgc agacgcggct tggcctgaag agtcactgat ctgaacaaac cagacatgcg    2400

ccagaagtcc tggtcttcca gccaggaggc ggaagaatac ttgtagagag ccactgccag    2460

gcggttgttt tctttcttga ggaacttggt aaccataaac tcgcttgggg taaaggagtc    2520

ttccccgtag ccgacgaagt ggccgttcag ccagacatag atggctgttg ccgccccgtc    2580

aaacttcaag ctgacttcct tgtcccagaa agcttcatcc aggtcaaagt atctgacata    2640

agaagcgagc ggatttttgc ttggaatttg gggcgggaaa atctcctcac tgccgtccca    2700

tggatattgg acgttgacat actggggctg gccaaagcct tgcagttcca ggttgccggg    2760

tactttgact gacttaaaat tgctatcgtc aaagtcttct gcatagaagt tgactggtcc    2820

ctggccgttt tcagcgtagt caatcagcca gtccccgtcc agggactgca ctaaactgga    2880

cttgccctcc tccagttctt cctggctttg gaaggactca tggtcggagt gcgggggaat    2940

tgtattgact tcgtaaactt ccgggtcagt cagccaggcc aggtctgcct ggtcaactct    3000

tttttctttt actaacttat tgctcat                                        3027


