<110>    Lugen Sci Co., Ltd.

<120>    Human transformed chondrocyte cell line mediated drug screening
         system for cartilage disease remedy

<130>    MPCT19-104

<150>    KR 10-2018-0106190
<151>    2018-09-05

<150>    KR 10-2019-0105987
<151>    2019-08-28

<160>    12

<170>    KoPatentIn 3.0

<210>    1
<211>    394
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Col2a1 promoter


<400>    1
cccgggtttg gggggcaggg gccatctctg cgccccgccc gatcaggcca ctcggcgcac         60

taggggtgga gggcgggaag cgtgactccc agagaggggg gtccggcttg ggcaggtgcg        120

ggcactggca gggcccaggc gggctccggg ggcgggcggt tcaggttaca gcccagctgg        180

gggcaggggg cggcccgcgg tttgggcgag ttcgccagcc tcgaaagggg ccgggcgcat        240

ataacgggcg ccgcggcggg gagaagacgc agagcgctgc tgggctgccg ggtctcccgc        300

ttcctcctcc tgctccaagg gcctcctgca tgagggcgcg gtagagaccc ggacccgcgc        360

cgtgctcctg ccgtttcgct gcgctccgcc cggg                                    394


<210>    2
<211>    725
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Col2a1 Promoter Enhancer


<400>    2
gaattcggcc acacccctcc tctccatctt tccacgagtt tgagaaactt actggcggcg         60

gcgtctttga ccctcatctg catttcagag ccctcgcctc cgaaagtgcc cctggctcag        120

gggagagatc tcaatcctcc tttgtgaggc ttgtttgcat tgggagattg gcagcgatgg        180

cttccagatg gggctgaaac gctgcccgta tttatttaaa ctggttcctc gcggagacct        240

gtgaatcggg ctctgtgtgc gctcgagaaa agccccattc atgagagacg aggtccagtg        300

ggttctctcg tactcccaga ccccctctcc cacaatgccc ccctgtgccc gcccgccgcc        360

acctctcggc tccagccctg cgcagagcgg cggtgaagca aaacagttcc ccgaaagagg        420

tagcttttta attggcttgc cacaaagaat cacttatacg gccctgcggt aatgagggga        480

accggatcag gcgcgccggg atgctatcgg cagccgtttt ggagcagcaa ttatggtggt        540

gctgggctcc tccgtccaca cctaggggat ccggttacgg cgctggctcc tttctggggc        600

agtcatttaa tcccactttt cactctcccg gtgtctgtga gcgagccgtg tccagagccg        660

cagccacaga gtcactcagc ggctcttaca cccagcgcag cctggccccg cccctgcagc        720

ccggg                                                                    725


<210>    3
<211>    8884
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pCDH-ENS-C2P-copGFP-Puro


<400>    3
acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca         60

acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta        120

cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga        180

attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc        240

tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta        300

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact        360

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg        420

cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct        480

tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt        540

gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag        600

aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt        660

aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt        720

agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg        780

atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag        840

gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag        900

taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg        960

acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag       1020

cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag       1080

ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc       1140

tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga       1200

gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc       1260

aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg       1320

gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata       1380

aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca       1440

attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg       1500

aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa       1560

attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa       1620

tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt       1680

ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag       1740

gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt       1800

taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca       1860

taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt       1920

tatcgatact agtggccaca cccctcctct ccatctttcc acgagtttga gaaacttact       1980

ggcggcggcg tctttgaccc tcatctgcat ttcagagccc tcgcctccga aagtgcccct       2040

ggctcagggg agagatctca atcctccttt gtgaggcttg tttgcattgg gagattggca       2100

gcgatggctt ccagatgggg ctgaaacgct gcccgtattt atttaaactg gttcctcgcg       2160

gagacctgtg aatcgggctc tgtgtgcgct cgagaaaagc cccattcatg agagacgagg       2220

tccagtgggt tctctcgtac tcccagaccc cctctcccac aatgcccccc tgtgcccgcc       2280

cgccgccacc tctcggctcc agccctgcgc agagcggcgg tgaagcaaaa cagttccccg       2340

aaagaggtag ctttttaatt ggcttgccac aaagaatcac ttatacggcc ctgcggtaat       2400

gaggggaacc ggatcaggcg cgccgggatg ctatcggcag ccgttttgga gcagcaatta       2460

tggtggtgct gggctcctcc gtccacacct aggggatccg gttacggcgc tggctccttt       2520

ctggggcagt catttaatcc cacttttcac tctcccggtg tctgtgagcg agccgtgtcc       2580

agagccgcag ccacagagtc actcagcggc tcttacaccc agcgcagcct ggccccgccc       2640

ctgcagcccg ggtttggggg gcaggggcca tctctgcgcc ccgcccgatc aggccactcg       2700

gcgcactagg ggtggagggc gggaagcgtg actcccagag aggggggtcc ggcttgggca       2760

ggtgcgggca ctggcagggc ccaggcgggc tccgggggcg ggcggttcag gttacagccc       2820

agctgggggc agggggcggc ccgcggtttg ggcgagttcg ccagcctcga aaggggccgg       2880

gcgcatataa cgggcgccgc ggcggggaga agacgcagag cgctgctggg ctgccgggtc       2940

tcccgcttcc tcctcctgct ccaagggcct cctgcatgag ggcgcggtag agacccggac       3000

ccgcgccgtg ctcctgccgt ttcgctgcgc tccgcccggg tctagagcta gcatggagag       3060

cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg       3120

cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa       3180

caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat       3240

gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca       3300

cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt       3360

gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt       3420

ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa       3480

cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg       3540

caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt       3600

caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg       3660

cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa       3720

gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac       3780

cgccggaccc ggctccaccg gatctcgcta agcggccgcg aaggatctgc gatcgctccg       3840

gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg       3900

tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg       3960

tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg       4020

ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg       4080

ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga       4140

gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa       4200

gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac       4260

tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt       4320

tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gatgaccgag       4380

tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc       4440

gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc       4500

gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag       4560

gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa       4620

gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg       4680

gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg       4740

ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc       4800

gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc       4860

gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag       4920

gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat       4980

caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct       5040

tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg       5100

gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg       5160

cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt       5220

tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt       5280

gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg       5340

ggcactgaca attccgtggt ggttgtcggg gaaatcatcg tcctttcctt ggctgctcgc       5400

ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa       5460

tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg       5520

ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa       5580

gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac       5640

tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc       5700

tctggttaga ccaaatcgga gcctgggagc tctctggcta actagggaac ccactgctta       5760

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact       5820

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta       5880

gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg       5940

agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt       6000

tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg       6060

tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc       6120

tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc       6180

tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg       6240

cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat       6300

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag       6360

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg       6420

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc       6480

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc       6540

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa       6600

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt       6660

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg       6720

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg       6780

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag       6840

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc       6900

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa       6960

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg       7020

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc       7080

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac       7140

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg       7200

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt       7260

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt       7320

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa       7380

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga       7440

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt       7500

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg       7560

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga       7620

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga       7680

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg       7740

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc       7800

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc       7860

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca       7920

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac       7980

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg       8040

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc       8100

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg       8160

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac       8220

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat       8280

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata       8340

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa       8400

agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg       8460

tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat       8520

gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg       8580

tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga       8640

gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag       8700

aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc       8760

ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt       8820

aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa       8880

gctg                                                                    8884


<210>    4
<211>    9787
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pCDH-ENS-C2P-fLuc-Puro


<400>    4
acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca         60

acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta        120

cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga        180

attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc        240

tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta        300

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact        360

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg        420

cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct        480

tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt        540

gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag        600

aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt        660

aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt        720

agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg        780

atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag        840

gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag        900

taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg        960

acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag       1020

cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag       1080

ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc       1140

tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga       1200

gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc       1260

aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg       1320

gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata       1380

aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca       1440

attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg       1500

aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa       1560

attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa       1620

tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt       1680

ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag       1740

gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt       1800

taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca       1860

taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt       1920

tatcgatact agtggccaca cccctcctct ccatctttcc acgagtttga gaaacttact       1980

ggcggcggcg tctttgaccc tcatctgcat ttcagagccc tcgcctccga aagtgcccct       2040

ggctcagggg agagatctca atcctccttt gtgaggcttg tttgcattgg gagattggca       2100

gcgatggctt ccagatgggg ctgaaacgct gcccgtattt atttaaactg gttcctcgcg       2160

gagacctgtg aatcgggctc tgtgtgcgct cgagaaaagc cccattcatg agagacgagg       2220

tccagtgggt tctctcgtac tcccagaccc cctctcccac aatgcccccc tgtgcccgcc       2280

cgccgccacc tctcggctcc agccctgcgc agagcggcgg tgaagcaaaa cagttccccg       2340

aaagaggtag ctttttaatt ggcttgccac aaagaatcac ttatacggcc ctgcggtaat       2400

gaggggaacc ggatcaggcg cgccgggatg ctatcggcag ccgttttgga gcagcaatta       2460

tggtggtgct gggctcctcc gtccacacct aggggatccg gttacggcgc tggctccttt       2520

ctggggcagt catttaatcc cacttttcac tctcccggtg tctgtgagcg agccgtgtcc       2580

agagccgcag ccacagagtc actcagcggc tcttacaccc agcgcagcct ggccccgccc       2640

ctgcagcccg ggtttggggg gcaggggcca tctctgcgcc ccgcccgatc aggccactcg       2700

gcgcactagg ggtggagggc gggaagcgtg actcccagag aggggggtcc ggcttgggca       2760

ggtgcgggca ctggcagggc ccaggcgggc tccgggggcg ggcggttcag gttacagccc       2820

agctgggggc agggggcggc ccgcggtttg ggcgagttcg ccagcctcga aaggggccgg       2880

gcgcatataa cgggcgccgc ggcggggaga agacgcagag cgctgctggg ctgccgggtc       2940

tcccgcttcc tcctcctgct ccaagggcct cctgcatgag ggcgcggtag agacccggac       3000

ccgcgccgtg ctcctgccgt ttcgctgcgc tccgcccggg tctagagcta gcatggaaga       3060

cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg       3120

agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac       3180

agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt       3240

ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga       3300

aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc       3360

gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc       3420

taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt       3480

accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc       3540

gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc       3600

agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg       3660

gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag       3720

agatcctatt tttggcaatc aaatcgttcc ggatactgcg attttaagtg ttgtcccatt       3780

ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt       3840

cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca       3900

aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa       3960

atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt       4020

cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac       4080

tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg       4140

taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg       4200

cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa       4260

caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat       4320

agcttactgg gacgaagacg aacacttctt catagttgac cgcttgaagt ctttaattaa       4380

atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa       4440

catcttcgac gcgggcgtgg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc       4500

cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc       4560

cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc       4620

gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa       4680

gaagggcgga aagtccaaat tgctcgagtg atgagcggcc gcgaaggatc tgcgatcgct       4740

ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag       4800

gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg       4860

tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag       4920

tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag       4980

gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt       5040

tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg       5100

taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta       5160

gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt       5220

cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagatgacc       5280

gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc       5340

ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac       5400

atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc       5460

aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc       5520

gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg       5580

ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg       5640

tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc       5700

gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc       5760

tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc       5820

gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagtcgac       5880

aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       5940

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt       6000

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg       6060

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact       6120

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct       6180

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg       6240

ttgggcactg acaattccgt ggtggttgtc ggggaaatca tcgtcctttc cttggctgct       6300

cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct       6360

caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct       6420

tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt       6480

taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg       6540

gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt       6600

ctctctggtt agaccaaatc ggagcctggg agctctctgg ctaactaggg aacccactgc       6660

ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg       6720

actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta       6780

gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga       6840

gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa       6900

atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca       6960

atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca       7020

ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc       7080

ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga       7140

cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac       7200

aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt       7260

gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc       7320

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg       7380

ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt       7440

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa       7500

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc       7560

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag       7620

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt       7680

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg       7740

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg       7800

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg       7860

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac       7920

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg       7980

gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt       8040

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg       8100

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc       8160

tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt       8220

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt       8280

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag       8340

tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt       8400

cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc       8460

gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc       8520

cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg       8580

ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac       8640

aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg       8700

atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc       8760

tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact       8820

gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc       8880

aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat       8940

acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc       9000

ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac       9060

tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa       9120

aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact       9180

catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg       9240

atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg       9300

aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag       9360

gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca       9420

catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc       9480

ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc       9540

agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag       9600

gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg       9660

atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg       9720

attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc       9780

caagctg                                                                 9787


<210>    5
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer of Col2a1


<400>    5
aaccagattg agagcatccg                                                     20


<210>    6
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer of Col2a1


<400>    6
accttcatgg cgtccaag                                                       18


<210>    7
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer of Sox9


<400>    7
acttgcacaa cgccgag                                                        17


<210>    8
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer of Sox9


<400>    8
ctggtacttg taatccgggt g                                                   21


<210>    9
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer of Col10a1


<400>    9
acgataccaa atgcccacag                                                     20


<210>    10
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer of Col10a1


<400>    10
gtaccttgct ctcctcttac tg                                                  22


<210>    11
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer of Gapdh


<400>    11
acatcgctca gacaccatg                                                      19


<210>    12
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer of Gapdh


<400>    12
tgtagttgag gtcaatgaag gg                                                  22
