                         SEQUENCE LISTING

<110>  Academia Sinica
       SHIH, Ming-Che
 
<120>  A MEDIUM COMPOSITION AND METHOD FOR CULTURING MESENCHYMAL STEM 
       CELLS

<130>  ACA0130WO

<150>  62/726,586
<151>  2018-09-04

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  314
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Pro Pro Gln Val Leu Ala Phe Gly Leu Leu Leu Ala Ala Ala 
1               5                   10                  15      


Thr Ala Thr Phe Ala Ala Ala Gln Glu Glu Cys Val Cys Glu Asn Tyr 
            20                  25                  30          


Lys Leu Ala Val Asn Cys Phe Val Asn Asn Asn Arg Gln Cys Gln Cys 
        35                  40                  45              


Thr Ser Val Gly Ala Gln Asn Thr Val Ile Cys Ser Lys Leu Ala Ala 
    50                  55                  60                  


Lys Cys Leu Val Met Lys Ala Glu Met Asn Gly Ser Lys Leu Gly Arg 
65                  70                  75                  80  


Arg Ala Lys Pro Glu Gly Ala Leu Gln Asn Asn Asp Gly Leu Tyr Asp 
                85                  90                  95      


Pro Asp Cys Asp Glu Ser Gly Leu Phe Lys Ala Lys Gln Cys Asn Gly 
            100                 105                 110         


Thr Ser Met Cys Trp Cys Val Asn Thr Ala Gly Val Arg Arg Thr Asp 
        115                 120                 125             


Lys Asp Thr Glu Ile Thr Cys Ser Glu Arg Val Arg Thr Tyr Trp Ile 
    130                 135                 140                 


Ile Ile Glu Leu Lys His Lys Ala Arg Glu Lys Pro Tyr Asp Ser Lys 
145                 150                 155                 160 


Ser Leu Arg Thr Ala Leu Gln Lys Glu Ile Thr Thr Arg Tyr Gln Leu 
                165                 170                 175     


Asp Pro Lys Phe Ile Thr Ser Ile Leu Tyr Glu Asn Asn Val Ile Thr 
            180                 185                 190         


Ile Asp Leu Val Gln Asn Ser Ser Gln Lys Thr Gln Asn Asp Val Asp 
        195                 200                 205             


Ile Ala Asp Val Ala Tyr Tyr Phe Glu Lys Asp Val Lys Gly Glu Ser 
    210                 215                 220                 


Leu Phe His Ser Lys Lys Met Asp Leu Thr Val Asn Gly Glu Gln Leu 
225                 230                 235                 240 


Asp Leu Asp Pro Gly Gln Thr Leu Ile Tyr Tyr Val Asp Glu Lys Ala 
                245                 250                 255     


Pro Glu Phe Ser Met Gln Gly Leu Lys Ala Gly Val Ile Ala Val Ile 
            260                 265                 270         


Val Val Val Val Ile Ala Val Val Ala Gly Ile Val Val Leu Val Ile 
        275                 280                 285             


Ser Arg Lys Lys Arg Met Ala Lys Tyr Glu Lys Ala Glu Ile Lys Glu 
    290                 295                 300                 


Met Gly Glu Met His Arg Glu Leu Asn Ala 
305                 310                 


<210>  2
<211>  265
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Pro Pro Gln Val Leu Ala Phe Gly Leu Leu Leu Ala Ala Ala 
1               5                   10                  15      


Thr Ala Thr Phe Ala Ala Ala Gln Glu Glu Cys Val Cys Glu Asn Tyr 
            20                  25                  30          


Lys Leu Ala Val Asn Cys Phe Val Asn Asn Asn Arg Gln Cys Gln Cys 
        35                  40                  45              


Thr Ser Val Gly Ala Gln Asn Thr Val Ile Cys Ser Lys Leu Ala Ala 
    50                  55                  60                  


Lys Cys Leu Val Met Lys Ala Glu Met Asn Gly Ser Lys Leu Gly Arg 
65                  70                  75                  80  


Arg Ala Lys Pro Glu Gly Ala Leu Gln Asn Asn Asp Gly Leu Tyr Asp 
                85                  90                  95      


Pro Asp Cys Asp Glu Ser Gly Leu Phe Lys Ala Lys Gln Cys Asn Gly 
            100                 105                 110         


Thr Ser Met Cys Trp Cys Val Asn Thr Ala Gly Val Arg Arg Thr Asp 
        115                 120                 125             


Lys Asp Thr Glu Ile Thr Cys Ser Glu Arg Val Arg Thr Tyr Trp Ile 
    130                 135                 140                 


Ile Ile Glu Leu Lys His Lys Ala Arg Glu Lys Pro Tyr Asp Ser Lys 
145                 150                 155                 160 


Ser Leu Arg Thr Ala Leu Gln Lys Glu Ile Thr Thr Arg Tyr Gln Leu 
                165                 170                 175     


Asp Pro Lys Phe Ile Thr Ser Ile Leu Tyr Glu Asn Asn Val Ile Thr 
            180                 185                 190         


Ile Asp Leu Val Gln Asn Ser Ser Gln Lys Thr Gln Asn Asp Val Asp 
        195                 200                 205             


Ile Ala Asp Val Ala Tyr Tyr Phe Glu Lys Asp Val Lys Gly Glu Ser 
    210                 215                 220                 


Leu Phe His Ser Lys Lys Met Asp Leu Thr Val Asn Gly Glu Gln Leu 
225                 230                 235                 240 


Asp Leu Asp Pro Gly Gln Thr Leu Ile Tyr Tyr Val Asp Glu Lys Ala 
                245                 250                 255     


Pro Glu Phe Ser Met Gln Gly Leu Lys 
            260                 265 


<210>  3
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  3

Ala Gly Val Ile Ala Val Ile Val Val Val Val Ile Ala Val Val Ala 
1               5                   10                  15      


Gly Ile Val Val Leu Val Ile 
            20              


<210>  4
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  4

Ser Arg Lys Lys Arg Met Ala Lys Tyr Glu Lys Ala Glu Ile Lys Glu 
1               5                   10                  15      


Met Gly Glu Met His Arg Glu Leu Asn Ala 
            20                  25      


<210>  5
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (Oct4 promoter)

<400>  5
agcaactggt ttgtgaggtg tccggtgac                                         29


<210>  6
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (Oct4 promoter)

<400>  6
ctccccaatc ccaccctcta gccttgac                                          28


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (Sox2 promoter)

<400>  7
ttttcgtttt tagggtaagg tactgggaag                                        30


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (Sox2 promoter)

<400>  8
ccacgtgaat aatcctatat gcatcacaat                                        30


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer (beta-actin promoter)

<400>  9
aaatgctgca ctgtgcggcg                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer (beta-actin promoter)

<400>  10
aggcaacttt cggaacggcg                                                   20


