                         SEQUENCE LISTING

<110>  NUSEED PTY LTD.
 
<120>  METHODS OF IDENTIFYING DHA CANOLA NS-B50027-4

<130>  87376_0013_PCT

<150>  US 62/839,482
<151>  2019-04-26

<150>  US 62/729,805
<151>  2018-09-11

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B. napus fragment of A02 replaced by transgenic insert

<400>  1
gtagcacgac aagtt                                                        15


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B. napus fragment of A05 replaced by transgenic insert

<400>  2
cacggtggag gtcaccatgt                                                   20


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for junction of T-DNA insert on chromosome A05 
       (A05-286F)

<400>  3
gaacaacaag gaacagagca acgt                                              24


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for junction of T-DNA insert on on chromosome A05 
       (A05-286R)

<400>  4
gacaatctgc tagtggatct ccca                                              24


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for junction of T-DNA insert on on chromosome A02 
       (A05-282F)

<400>  5
cagatcttcc aaggcctcgt                                                   20


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for junction of T-DNA insert on chromosome A02 
       (A05-282R)

<400>  6
cgctcttata ctgcactggt tag                                               23


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for internal reference gene HMG (hmg99F; control)

<400>  7
ggtcgtcctc ctaaggcgaa ag                                                22


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for internal reference gene HMG (hmg99R; control)

<400>  8
cttcttcggc ggtcgtccac                                                   20


<210>  9
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A02 genomic/transgene junction sequence used to design primers

<400>  9
ctccgccgcc aacaaggctt gtagttaata ggaatcattc agggattgtg attccgggca       60

gtagtaatta ataatatagt attagtatac agaacctctt atttagctaa aagattatgt      120

tcttaatgtt gataagaagt ttgagaaaca aatataattg agcttctgat tagttgatcg      180

taattggtc                                                              189


<210>  10
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A05 genomic/transgene junction sequence used to design primers

<400>  10
ttttcgtcga gttgctgaaa ctggacccaa gccagtgtac ggcgcaggag gtactttaag       60

cttataaccc tttgtctatc ctttggctag cggctaatgt tgatgaactt ttttattcaa      120

ccgttggcta aggtaacact gatagtttaa actgaaggcg ggaaacgaca atctgctagt      180

ggatctccca gtcacgacgt tgtaaaacgg gcgccccgcg gaaagc                     226


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reference probe sequence for quantitative detection (hmg-P)

<400>  11
cggagccact cggtgccgca actt                                              24


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for A05up2 assay (A05up2F)

<400>  12
tctatccttt ggctagcggc                                                   20


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for A05up2 assay (A05up2R)

<400>  13
tgactgggag atccactagc a                                                 21


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe sequence for A05up2 assay (A05up2P)

<400>  14
caaccgttgg ctaaggtaac actga                                             25


<210>  15
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for A02dn2 assay (A02dn2F)

<400>  15
acaaggcttg tagttaatag gaatca                                            26


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for A02dn2 assay (A02dn2R)

<400>  16
acgatcaact aatcagaagc tcaatt                                            26


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe sequence for A02dn2 assay (A02dn2P)

<400>  17
tcagggattg tgattccggg ca                                                22


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for junction between T-DNA and genomic DNA on 
       Chromosome A05 (A05-200F)

<400>  18
tgttgtggtg gtgacgattt                                                   20


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for junction between T-DNA and genomic DNA on 
       Chromosome A05 (A05-200R)

<400>  19
tccactagca gattgtcgtt t                                                 21


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for junction between T-DNA and genomic DNA on 
       Chromosome A02 (A02-258F)

<400>  20
cattgagcag tgaacaccaa g                                                 21


<210>  21
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for junction between T-DNA and genomic DNA on 
       Chromosome A02 (A02-258R)

<400>  21
cagtttaaac tatcagtgtt tgaacac                                           27


<210>  22
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for internal reference gene HMG as control 
       (Hmg206F)

<400>  22
tccttccgtt tcctcgcc                                                     18


<210>  23
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for internal reference gene HMG as control 
       (Hmg206R)

<400>  23
ttccacgccc tctccgct                                                     18


<210>  24
<211>  320
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgene junction region of NS-B50027-4 chromosome A02 for assay
       A02-282

<400>  24
ctgattctgc ccgttcgact cagatcttcc aaggcctcgt ctccgagtcc gctgcttctc       60

gccgcgccga tcacttctcc gccgccaaca aggcttgtag ttaataggaa tcattcaggg      120

attgtgattc cgggcagtag taattaataa tatagtatta gtatacagaa cctcttattt      180

agctaaaaga ttatgttctt aatgttgata agaagtttga gaaacaaata taattgagct      240

tctgattagt tgatcgtaat tggtcattaa taattgtatc taaccagtgc agtataagag      300

cgtataagag catcttcaaa                                                  320


<210>  25
<211>  380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgene junction region of NS-B50027-4 chromosome A05 for assay
       A05-286

<400>  25
tttctttacc caaatctagt tcttgagagg atgaagcatc accgaacagt tctgcaacta       60

tccctcaaaa gctttaaaat gaacaacaag gaacagagca acgttccaaa gatcccaaac      120

gaaacatatt atctatacta atactatatt attaattact actgcccgga atcacaatcc      180

ctgaatgatt cctattaact acaagccttg ttggcggcgg agaagtgatc ggcgcggcga      240

gaagcagcgg actcggagac gaggccttgg aagatctgag tcgaacgggc ggtaccgcgg      300

gcccgtttta caacgtcgtg actgggagat ccactagcag attgtcgttt cccgccttca      360

gtttaaacta tcagtgtttg                                                  380


<210>  26
<211>  479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgene junction region of NS-B50027-4 chromosome A02 for assay
       A02-258

<400>  26
ctacgatggt actcaatgcg gctaaggatt gatgaagatg gagatgttgc agatgagttc       60

ttggaagatg ataactgtaa gactttgccc agaaaatgca aaacaaaagc tgcaaaagtg      120

agaggtttag tgatatcttc tgatgggaaa cttcagccat taatgcattg agcagtgaac      180

accaaggata aatatttact gattagtgtg tgattgaatc aaagaaaggt tagaatctgg      240

ttttcattta gccattcaat ctcgatgtaa aatcggttag attctggttg ttgatacttg      300

agaacttgaa atgttttgta actgtgaatt ttgttttgaa aatagacaag tgaatctgtt      360

tgggttgtgt gaaaacgtgt gagcaattgt tggaggtgtt caaacactga tagtttaaac      420

tgaaggcggg aaacgacaat ctgctagtgg atctcccagt cacgacgttg taaaacggg       479


<210>  27
<211>  640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Transgene junction region of NS-B50027-4 chromosome A05 for assay
       A05-200

<400>  27
taatcatgga ggagaagaag gatttttata accatgtctc tgttgcttgc tgcttaaacc       60

ctatataatt ggaaactttt tgttttagtc tgtgtctgaa agtttctata ttcgtattgt      120

ctattttgta aagactaaaa caaaaatgcc tatttttagt ttgttcttgt tccgcaacac      180

cgttactgaa ctcttcgttc acttaaacag tttgtgtgtg tgagaaacag cgtaatgagc      240

tgctttggtt gttgtggtgg tgacgatttt cgtcgagttg ctgaaactgg acccaagcca      300

gtgtacggcg caggaggtac tttaagctta taaccctttg tctatccttt ggctagcggc      360

taatgttgat gaactttttt attcaaccgt tggctaaggt aacactgata gtttaaactg      420

aaggcgggaa acgacaatct gctagtggat ctcccagtca cgacgttgta aaacgggcgc      480

cccgcggaaa gcttgcggcc gcggtaccgc ccgttcgact cagatcttcc aaggcctcgt      540

ctccgagtcc gctgcttctc gccgcgccga tcacttctcc gccgccaaca aggcttgtag      600

ttaataggaa tcattcaggg attgtgattc cgggcagtag                            640


