                         SEQUENCE LISTING

<110>  Universitt fr Bodenkultur Wien
       ACIB GmbH
 
<120>  DUAL VECTOR SYSTEM FOR IMPROVED PRODUCTION OF PROTEINS IN ANIMAL 
       CELLS

<130>  BK020P

<160>  31    

<170>  PatentIn version 3.5

<210>  1
<211>  344
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7amiR fragment

<400>  1
taatacgact cactataggg ctgcaggtct atagatagcg gtttttcggc aatatacact       60

tggctcaatt tattatcgcc gtgtgcgatg cgcaagttgg ccacccggcc gttattcagc      120

tttacgttta attgtttgtt ctcgtcggat ccgaattcct cgagtctaga aaatttaatg      180

cattcgtcca ataaagataa aacagtatga gcaaaacgat aagtaacacg attccccaca      240

tgatttgttt taatttacaa tttcaattcc aatgagattt aggttgtgca ggtaccctag      300

cataacccct tggggcctct aaacgggtct tgaggggttt tttg                       344


<210>  2
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1A stem loop

<400>  2
tcagctttac gtttaattgt ttgttctcgt ctaaggctac gtctatactg ctctatccta       60

aactggatga tatagacgtt gtggccttga aatttaatgc attcgtccaa taaagataaa      120


<210>  3
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1As stem loop

<400>  3
tcagctttac gtttaattgt ttgttctcgt ctaaacactc tcagtaactg cgactcccta       60

aactgggatc ttactgagac aggtgtttga aatttaatgc attcgtccaa taaagataaa      120


<210>  4
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1B stem loop

<400>  4
ggatcctaag gccacaacgt ctatatcatc ctaaactgga tgatatagac gttgtggcct       60

tatctaga                                                                68


<210>  5
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1Bs stem loop

<400>  5
ggatcctaaa cactctctcg ggtaaaatcc ctaaactggg attttacccg agagagtgtt       60

tatctaga                                                                68


<210>  6
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1C stem loop

<400>  6
ggatcctaac agccacaacg tctatatcat gcctaaactg gcatgatata gacgttgtgg       60

ctgttatcta ga                                                           72


<210>  7
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1Cs stem loop

<400>  7
ggatcctaaa cacctctctc aggtaaaatc gcctaaactg gcgattttac ctgagagagg       60

tgtttatcta ga                                                           72


<210>  8
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1D stem loop

<400>  8
ggatcctgag cgtaacagcc acaacgtcta tatcatgcct aaactggcat gatatagacg       60

ttgtggctgt tacattcatc taga                                              84


<210>  9
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amiR-1Ds stem loop

<400>  9
ggatcctgag cgtaaacacc tctctcaggt aaaatcgcct aaactggcga ttttacctga       60

gagaggtgtt tacattcatc taga                                              84


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
ggcacaagct ggagtacaac                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  11
agttcacctt gatgccgttc                                                   20


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
tcacttctgg aatgggtag                                                    19


<210>  13
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  13
gactataagg accacgacgg agactacaag gatcatgata ttgattacaa agacgatgac       60

gataag                                                                  66


<210>  14
<211>  21
<212>  DNA
<213>  Sendai virus

<400>  14
ccaaagaaga agcggaaggt c                                                 21


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
cggcgtgagt atgattctca aa                                                22


<210>  16
<211>  164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7P-gRNA 1-T7T

<400>  16
taatacgact cactataggg ggtgttatga acttcgaaga gttttagagc tagaaatagc       60

aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcctag      120

cataacccct tggggcctct aaacgggtct tgaggggttt tttg                       164


<210>  17
<211>  162
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7P-gRNA 2-T7T

<400>  17
taatacgact cactataggg tgaaggtcgt ccatacgagt tttagagcta gaaatagcaa       60

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcctagca      120

taaccccttg gggcctctaa acgggtcttg aggggttttt tg                         162


<210>  18
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  promoter

<400>  18
taatacgact cactata                                                      17


<210>  19
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS

<400>  19

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  20
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  20
atgagcagac acgcagcttt t                                                 21


<210>  21
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  21
tcacttctgg aatgggtag                                                    19


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter sequence

<400>  22
taatacgact cactataggg                                                   20


<210>  23
<211>  2688
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNAP sequence with NLS

<400>  23
atgttccttg aacctcctaa gaagaagaga aaagtcgaga acacgattaa catcgctaag       60

aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac      120

ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa      180

gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct      240

gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg      300

tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa      360

atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt      420

gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag      480

gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa      540

caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct      600

gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac      660

tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc      720

ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct      780

gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc      840

caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct      900

aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac      960

gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg     1020

aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg     1080

gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac     1140

atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac     1200

aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt     1260

gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct     1320

gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa     1380

ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg     1440

ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac     1500

atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg     1560

ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat     1620

aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg     1680

atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag     1740

gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat     1800

gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa     1860

gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt     1920

gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa     1980

caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct gatgttcact     2040

cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg     2100

gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct     2160

gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta     2220

actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac     2280

ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag     2340

attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt     2400

agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca     2460

ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg     2520

cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag     2580

ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt     2640

aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaa                  2688


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 T antigen NLS sequence

<400>  24
cctaagaaga agagaaaagt c                                                 21


<210>  25
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  terminator sequence

<400>  25
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttg                    48


<210>  26
<211>  4272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9

<400>  26
atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat       60

gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc      120

gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc      180

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac      240

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc      300

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat      360

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg      420

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac      480

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa      540

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg      600

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      660

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc      720

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg      780

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg      840

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat      900

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      960

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg     1020

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg     1080

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag     1140

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc     1200

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa     1260

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag     1320

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc     1380

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag     1440

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga     1500

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg     1560

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac     1620

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat     1680

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc     1740

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg     1800

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc     1860

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc     1920

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg     1980

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac     2040

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg     2100

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat     2160

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc     2220

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac     2280

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg     2340

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc     2400

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg     2460

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg     2520

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat     2580

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc     2640

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac     2700

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac     2760

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc     2820

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg     2880

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact     2940

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag     3000

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac     3060

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac     3120

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3180

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac     3240

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct     3300

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc     3360

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag     3420

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc     3480

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat     3540

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa     3600

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt     3660

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac     3720

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag     3780

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac     3840

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag     3900

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc     3960

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc     4020

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct     4080

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag     4140

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac     4200

ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa     4260

aagaaaaagt aa                                                         4272


<210>  27
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleoplasmin NLS

<400>  27
aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaag                    48


<210>  28
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
gcttacagag acgaggtta                                                    19


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NLS

<400>  29

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  30
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gp64

<400>  30
ggatcctaag ctgcgtgtct gctcattaaa gcctaaactg gctttaatga gcagacacgc       60

agcttatcta ga                                                           72


<210>  31
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gp64scr

<400>  31
ggatcctaag tcatcgcatt gtctggacta gcctaaactg gctagtccag acaatgcgat       60

gacttatcta ga                                                           72


