<110>    Medicinal Bioconvergence Research Center

<120>    Antibody specifically binding to the N-terminal region of lysyl-
         tRNA synthetase exposed at cell outer membrane

<130>    OP19-0106/PCT

<150>    KR 10-2018-0111046
<151>    2018-09-17

<160>    251

<170>    KoPatentIn 3.0

<210>    1
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H1 amino acid


<400>    1
Ser Tyr Asp Met Ser
  1               5


<210>    2
<211>    15
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H1 DNA


<400>    2
agttatgata tgagc                                                          15


<210>    3
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 amino acid


<400>    3
Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    4
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 DNA


<400>    4
gcgatctctt atgataatgg taatacatat tacgctgatt ctgtaaaagg t                  51


<210>    5
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H3 amino acid


<400>    5
Met Ala Leu Asp Phe Asp Tyr
  1               5        


<210>    6
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H3 DNA


<400>    6
atggcgcttg atttcgacta c                                                   21


<210>    7
<211>    13
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L1 amino acid


<400>    7
Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn Tyr Val Thr
  1               5                  10            


<210>    8
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L1 DNA


<400>    8
actggctctt catctaatat tggcagtaat tatgtcacc                                39


<210>    9
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L2 amino acid


<400>    9
Asp Asn Ser Asn Arg Pro Ser
  1               5        


<210>    10
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L2 DNA


<400>    10
gataatagta atcggccaag c                                                   21


<210>    11
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L3 amino acid


<400>    11
Ala Ser Trp Asp Asp Ser Leu Ser Ala Tyr Val
  1               5                  10    


<210>    12
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L3 DNA


<400>    12
gcttcttggg atgatagcct gagtgcttat gtc                                      33


<210>    13
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L3 mutant 1 amino acid


<400>    13
Ala Ser Phe Ser Asp Glu Leu Gly Ala Tyr Val
  1               5                  10    


<210>    14
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L3 mutant 1 DNA


<400>    14
gcttctttta gtgatgagtt gggggcttat gtc                                      33


<210>    15
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L3 mutant 2 amino acid


<400>    15
Ser Ser Phe Ser Asp Glu Leu Gly Ala Tyr Val
  1               5                  10    


<210>    16
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L3 mutant 2 DNA


<400>    16
tcttctttta gtgatgagtt gggggcttat gtc                                      33


<210>    17
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 1 amino acid


<400>    17
Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    18
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 1 DNA


<400>    18
gcgatctcgc cgcagatggg tcgggtgtat tacgctgatt ctgtaaaagg t                  51


<210>    19
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 2 amino acid


<400>    19
Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    20
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 2 DNA


<400>    20
gcgatcgatc cgttgggggg taatatttat tacgctgatt ctgtaaaagg t                  51


<210>    21
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 3 amino acid


<400>    21
Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    22
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 3 DNA


<400>    22
gcgatctctc cgtattcggg taggatttat tacgctgatt ctgtaaaagg t                  51


<210>    23
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 4 amino acid


<400>    23
Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    24
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 4 DNA


<400>    24
gcgatcgggg ctgatggggg tccgtcttat tacgctgatt ctgtaaaagg t                  51


<210>    25
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H3 mutant 1 amino acid


<400>    25
Leu Ala Leu Asp Phe Asp Tyr
  1               5        


<210>    26
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H3 mutant 1 DNA


<400>    26
ctggcgcttg atttcgacta c                                                   21


<210>    27
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L2 mutant 1 amino acid


<400>    27
Ser Asn Asn Gln Arg Pro Ser
  1               5        


<210>    28
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L2 mutant 1 DNA


<400>    28
agtaataatc agcggccaag c                                                   21


<210>    29
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR L2 mutant 2 amino acid


<400>    29
Arg Asn Asn Gln Arg Pro Ser
  1               5        


<210>    30
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR L2 mutant 2 DNA


<400>    30
cggaataatc agcggccaag c                                                   21


<210>    31
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH


<400>    31
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    32
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH


<400>    32
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    33
<211>    110
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VL


<400>    33
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp Asp Ser Leu
                 85                  90                  95 

Ser Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu
            100                 105                 110


<210>    34
<211>    330
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VL


<400>    34
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccgg        240

tccgaggatg aggctgatta ttactgtgct tcttgggatg atagcctgag tgcttatgtc        300

ttcggcggag gcaccaagct gacggtccta                                         330


<210>    35
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 1


<400>    35
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    36
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 1


<400>    36
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    37
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 2


<400>    37
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    38
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 2


<400>    38
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    39
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 3


<400>    39
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    40
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 3


<400>    40
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    41
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 4


<400>    41
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    42
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 4


<400>    42
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    43
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 5


<400>    43
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    44
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 5


<400>    44
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    45
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 6


<400>    45
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    46
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 6


<400>    46
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    47
<211>    116
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 7


<400>    47
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser
        115    


<210>    48
<211>    348
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VH mutant 7


<400>    48
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctca                     348


<210>    49
<211>    110
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 1


<400>    49
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu
            100                 105                 110


<210>    50
<211>    330
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 1


<400>    50
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgtgct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta                                         330


<210>    51
<211>    110
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 2


<400>    51
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu
            100                 105                 110


<210>    52
<211>    330
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 2


<400>    52
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta                                         330


<210>    53
<211>    110
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 3


<400>    53
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu
            100                 105                 110


<210>    54
<211>    330
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 3


<400>    54
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat agtaataatc agcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta                                         330


<210>    55
<211>    110
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 4


<400>    55
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Arg Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu
            100                 105                 110


<210>    56
<211>    330
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 VL mutant 4


<400>    56
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat cggaataatc agcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta                                         330


<210>    57
<211>    15
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Linker for scFv


<400>    57
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
  1               5                  10                  15


<210>    58
<211>    45
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Linker for scFv


<400>    58
ggtggaggcg gttcaggcgg aggtggatcc ggcggtggcg gatcg                         45


<210>    59
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 scFv (VH+linker+VL)


<400>    59
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Arg Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp
    210                 215                 220 

Asp Ser Leu Ser Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    60
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 scFv (VH+linker+VL)


<400>    60
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cggtccgagg atgaggctga ttattactgt        660

gcttcttggg atgatagcct gagtgcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    61
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-1 scFv (VH+linker+VL)


<400>    61
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    62
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-1 scFv (VH+linker+VL)


<400>    62
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

gcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    63
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-3 scFv (VH+linker+VL)


<400>    63
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    64
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-3 scFv (VH+linker+VL)


<400>    64
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    65
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-4 scFv (VH+linker+VL)


<400>    65
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    66
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-4 scFv (VH+linker+VL)


<400>    66
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

gcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    67
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-5 scFv (VH+linker+VL)


<400>    67
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    68
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-5 scFv (VH+linker+VL)


<400>    68
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    69
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-6 scFv (VH+linker+VL)


<400>    69
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    70
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-6 scFv (VH+linker+VL)


<400>    70
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    71
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-7 scFv (VH+linker+VL)


<400>    71
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    72
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-7 scFv (VH+linker+VL)


<400>    72
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    73
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-7 scFv (VH+linker+VL)


<400>    73
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    74
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-7 scFv (VH+linker+VL)


<400>    74
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    75
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-9 scFv (VH+linker+VL)


<400>    75
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    76
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-9 scFv (VH+linker+VL)


<400>    76
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    77
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8 scFv, N3-8-1 scFv (VH+linker+VL)


<400>    77
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    78
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8 scFv, N3-8-1 scFv (VH+linker+VL)


<400>    78
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    79
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-2 scFv (VH+linker+VL)


<400>    79
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    80
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-2 scFv (VH+linker+VL)


<400>    80
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatagtaata atcagcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    81
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-3 scFv (VH+linker+VL)


<400>    81
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Arg Asn Asn Gln Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    82
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-3 scFv (VH+linker+VL)


<400>    82
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatcggaata atcagcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    83
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-4 scFv (VH+linker+VL)


<400>    83
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    84
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-4 scFv (VH+linker+VL)


<400>    84
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatgataata gtaatcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    85
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-5 scFv (VH+linker+VL)


<400>    85
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    86
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-5 scFv (VH+linker+VL)


<400>    86
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatagtaata atcagcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    87
<211>    241
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3-8-6 scFv (VH+linker+VL)


<400>    87
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
        115                 120                 125 

Gly Gly Ser Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr
    130                 135                 140 

Pro Gly Gln Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile
145                 150                 155                 160 

Gly Ser Asn Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro
                165                 170                 175 

Lys Leu Leu Ile Tyr Arg Asn Asn Gln Arg Pro Ser Gly Val Pro Asp
            180                 185                 190 

Arg Phe Ser Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser
        195                 200                 205 

Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser
    210                 215                 220 

Asp Glu Leu Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val
225                 230                 235                 240 

Leu
   


<210>    88
<211>    723
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3-8-6 scFv (VH+linker+VL)


<400>    88
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagg tggaggcggt        360

tcaggcggag gtggatccgg cggtggcgga tcgcagtctg tgctgactca gccaccctca        420

gcgtctggga cccccgggca gagggtcacc atctcttgta ctggctcttc atctaatatt        480

ggcagtaatt atgtcacctg gtaccagcag ctcccaggaa cggcccccaa actcctcatc        540

tatcggaata atcagcggcc aagcggggtc cctgaccgat tctctggctc caagtctggc        600

acctcagcct ccctggccat cagtgggctc cagtccgagg atgaggctga ttattactgt        660

tcttctttta gtgatgagtt gggggcttat gtcttcggcg gaggcaccaa gctgacggtc        720

cta                                                                      723


<210>    89
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 IgG heavy chain


<400>    89
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    90
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 IgG heavy chain


<400>    90
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    91
<211>    217
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    N3 IgG light chain


<400>    91
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Arg
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Trp Asp Asp Ser Leu
                 85                  90                  95 

Ser Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Thr
            100                 105                 110 

Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
        115                 120                 125 

Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
    130                 135                 140 

Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145                 150                 155                 160 

Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
                165                 170                 175 

Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
            180                 185                 190 

Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
        195                 200                 205 

Thr Lys Ser Phe Asn Arg Gly Glu Cys
    210                 215        


<210>    92
<211>    651
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    N3 IgG light chain


<400>    92
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccgg        240

tccgaggatg aggctgatta ttactgtgct tcttgggatg atagcctgag tgcttatgtc        300

ttcggcggag gcaccaagct gacggtccta cgtacggtgg ctgcaccatc tgtcttcatc        360

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat        420

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt        480

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc        540

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc        600

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t                 651


<210>    93
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1


<400>    93
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    94
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1


<400>    94
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    95
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2


<400>    95
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    96
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2


<400>    96
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    97
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3


<400>    97
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    98
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3


<400>    98
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    99
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4


<400>    99
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    100
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4


<400>    100
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    101
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5


<400>    101
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    102
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5


<400>    102
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    103
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6


<400>    103
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    104
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6


<400>    104
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    105
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7


<400>    105
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    106
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7


<400>    106
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    107
<211>    217
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG light chain 1


<400>    107
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Thr
            100                 105                 110 

Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
        115                 120                 125 

Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
    130                 135                 140 

Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145                 150                 155                 160 

Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
                165                 170                 175 

Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
            180                 185                 190 

Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
        195                 200                 205 

Thr Lys Ser Phe Asn Arg Gly Glu Cys
    210                 215        


<210>    108
<211>    651
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG light chain 1


<400>    108
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgtgct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta cgtacggtgg ctgcaccatc tgtcttcatc        360

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat        420

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt        480

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc        540

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc        600

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t                 651


<210>    109
<211>    217
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG light chain 2


<400>    109
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Thr
            100                 105                 110 

Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu
        115                 120                 125 

Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro
    130                 135                 140 

Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly
145                 150                 155                 160 

Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr
                165                 170                 175 

Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His
            180                 185                 190 

Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val
        195                 200                 205 

Thr Lys Ser Phe Asn Arg Gly Glu Cys
    210                 215        


<210>    110
<211>    651
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG light chain 2


<400>    110
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta cgtacggtgg ctgcaccatc tgtcttcatc        360

ttcccgccat ctgatgagca gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat        420

aacttctatc ccagagaggc caaagtacag tggaaggtgg ataacgccct ccaatcgggt        480

aactcccagg agagtgtcac agagcaggac agcaaggaca gcacctacag cctcagcagc        540

accctgacgc tgagcaaagc agactacgag aaacacaaag tctacgcctg cgaagtcacc        600

catcagggcc tgagctcgcc cgtcacaaag agcttcaaca ggggagagtg t                 651


<210>    111
<211>    216
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG light chain 3


<400>    111
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Asp Asn Ser Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln
            100                 105                 110 

Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu
        115                 120                 125 

Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr
    130                 135                 140 

Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys
145                 150                 155                 160 

Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr
                165                 170                 175 

Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His
            180                 185                 190 

Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys
        195                 200                 205 

Thr Val Ala Pro Thr Glu Cys Ser
    210                 215    


<210>    112
<211>    648
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG light chain 3


<400>    112
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat gataatagta atcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta ggtcagccca aggctgcccc ctcggtcacg        360

ctcttcccac cctcctctga ggagcttcaa gccaacaagg ccacactggt gtgtctcata        420

agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag ccccgtcaag        480

gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc ggccagcagc        540

tacctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg ccaggtcacg        600

catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgctct                     648


<210>    113
<211>    216
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG light chain 4


<400>    113
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Ser Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln
            100                 105                 110 

Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu
        115                 120                 125 

Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr
    130                 135                 140 

Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys
145                 150                 155                 160 

Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr
                165                 170                 175 

Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His
            180                 185                 190 

Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys
        195                 200                 205 

Thr Val Ala Pro Thr Glu Cys Ser
    210                 215    


<210>    114
<211>    648
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG light chain 4


<400>    114
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat agtaataatc agcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta agtcagccca aggctgcccc ctcggtcacg        360

ctcttcccac cctcctctga ggagcttcaa gccaacaagg ccacactggt gtgtctcata        420

agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag ccccgtcaag        480

gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc ggccagcagc        540

tacctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg ccaggtcacg        600

catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgctct                     648


<210>    115
<211>    216
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG light chain 5


<400>    115
Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln
  1               5                  10                  15 

Arg Val Thr Ile Ser Cys Thr Gly Ser Ser Ser Asn Ile Gly Ser Asn
             20                  25                  30 

Tyr Val Thr Trp Tyr Gln Gln Leu Pro Gly Thr Ala Pro Lys Leu Leu
         35                  40                  45 

Ile Tyr Arg Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser
     50                  55                  60 

Gly Ser Lys Ser Gly Thr Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln
 65                  70                  75                  80 

Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ser Ser Phe Ser Asp Glu Leu
                 85                  90                  95 

Gly Ala Tyr Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln
            100                 105                 110 

Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu
        115                 120                 125 

Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr
    130                 135                 140 

Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys
145                 150                 155                 160 

Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr
                165                 170                 175 

Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His
            180                 185                 190 

Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys
        195                 200                 205 

Thr Val Ala Pro Thr Glu Cys Ser
    210                 215    


<210>    116
<211>    648
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG light chain 5


<400>    116
cagtctgtgc tgactcagcc accctcagcg tctgggaccc ccgggcagag ggtcaccatc         60

tcttgtactg gctcttcatc taatattggc agtaattatg tcacctggta ccagcagctc        120

ccaggaacgg cccccaaact cctcatctat cggaataatc agcggccaag cggggtccct        180

gaccgattct ctggctccaa gtctggcacc tcagcctccc tggccatcag tgggctccag        240

tccgaggatg aggctgatta ttactgttct tcttttagtg atgagttggg ggcttatgtc        300

ttcggcggag gcaccaagct gacggtccta ggtcagccca aggctgcccc ctcggtcacg        360

ctcttcccac cctcctctga ggagcttcaa gccaacaagg ccacactggt gtgtctcata        420

agtgacttct acccgggagc cgtgacagtg gcctggaagg cagatagcag ccccgtcaag        480

gcgggagtgg agaccaccac accctccaaa caaagcaaca acaagtacgc ggccagcagc        540

tacctgagcc tgacgcctga gcagtggaag tcccacagaa gctacagctg ccaggtcacg        600

catgaaggga gcaccgtgga gaagacagtg gcccctacag aatgctct                     648


<210>    117
<211>    28
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    KRS epitope F4


<400>    117
Glu Pro Lys Leu Ser Lys Asn Glu Leu Lys Arg Arg Leu Lys Ala Glu
  1               5                  10                  15 

Lys Lys Val Ala Glu Lys Glu Ala Lys Gln Lys Glu
             20                  25            


<210>    118
<211>    17
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 5 amino acid


<400>    118
Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val Lys
  1               5                  10                  15 

Gly
   


<210>    119
<211>    51
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CDR H2 mutant 5 DNA


<400>    119
gtgatctctt ctgatggtgg taatacatat tacgctgatt ctgtaaaagg t                  51


<210>    120
<211>    107
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    light chain constant region ver.1_amino acid


<400>    120
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu
  1               5                  10                  15 

Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe
             20                  25                  30 

Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln
         35                  40                  45 

Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser
     50                  55                  60 

Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu
 65                  70                  75                  80 

Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser
                 85                  90                  95 

Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys
            100                 105        


<210>    121
<211>    321
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    light chain constant region ver.1_DNA


<400>    121
cgtacggtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct         60

ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag        120

tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac        180

agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag        240

aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag        300

agcttcaaca ggggagagtg t                                                  321


<210>    122
<211>    106
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    light chain constant region ver.2_amino acid


<400>    122
Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser
  1               5                  10                  15 

Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp
             20                  25                  30 

Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro
         35                  40                  45 

Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn
     50                  55                  60 

Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys
 65                  70                  75                  80 

Ser His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val
                 85                  90                  95 

Glu Lys Thr Val Ala Pro Thr Glu Cys Ser
            100                 105    


<210>    123
<211>    318
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    light chain constant region ver.2_DNA


<400>    123
ggtcagccca aggctgcccc ctcggtcacg ctcttcccac cctcctctga ggagcttcaa         60

gccaacaagg ccacactggt gtgtctcata agtgacttct acccgggagc cgtgacagtg        120

gcctggaagg cagatagcag ccccgtcaag gcgggagtgg agaccaccac accctccaaa        180

caaagcaaca acaagtacgc ggccagcagc tacctgagcc tgacgcctga gcagtggaag        240

tcccacagaa gctacagctg ccaggtcacg catgaaggga gcaccgtgga gaagacagtg        300

gcccctacag aatgctct                                                      318


<210>    124
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 wild type_amino
         acid


<400>    124
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    125
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 wild type_DNA


<400>    125
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    126
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant TA_amino
         acid


<400>    126
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    127
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant TA_DNA


<400>    127
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcgcctacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    128
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant LALA_amino
         acid


<400>    128
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    129
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant LALA_DNA


<400>    129
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaagc cgccggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    130
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant
         LALATA_amino acid


<400>    130
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    131
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant LALATA_DNA


<400>    131
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaagc cgccggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcgcctacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    132
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant
         LALAPG_amino acid


<400>    132
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    133
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant LALAPG_DNA


<400>    133
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaagc cgccggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcggcgccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    134
<211>    330
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant
         LALAPGTA_amino acid


<400>    134
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys
  1               5                  10                  15 

Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr
 65                  70                  75                  80 

Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
            100                 105                 110 

Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro
        115                 120                 125 

Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys
    130                 135                 140 

Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145                 150                 155                 160 

Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
                165                 170                 175 

Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val Leu
            180                 185                 190 

His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn
        195                 200                 205 

Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly
    210                 215                 220 

Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu
225                 230                 235                 240 

Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr
                245                 250                 255 

Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn
            260                 265                 270 

Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe
        275                 280                 285 

Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
    290                 295                 300 

Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr
305                 310                 315                 320 

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
                325                 330


<210>    135
<211>    990
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG1 mutant
         LALAPGTA_DNA


<400>    135
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg         60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg        120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca        180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc        240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc        300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaagc cgccggggga        360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct        420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg        480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac        540

agcgcctacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag        600

gagtacaagt gcaaggtctc caacaaagcc ctcggcgccc ccatcgagaa aaccatctcc        660

aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc ccgggatgag        720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc        780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg        840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg        900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg        960

cagaagagcc tctccctgtc cccgggtaaa                                         990


<210>    136
<211>    327
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG4 wild type_amino
         acid


<400>    136
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
  1               5                  10                  15 

Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr
 65                  70                  75                  80 

Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro
            100                 105                 110 

Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
        115                 120                 125 

Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
    130                 135                 140 

Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp
145                 150                 155                 160 

Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe
                165                 170                 175 

Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
            180                 185                 190 

Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu
        195                 200                 205 

Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
    210                 215                 220 

Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
225                 230                 235                 240 

Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
                245                 250                 255 

Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
            260                 265                 270 

Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
        275                 280                 285 

Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
    290                 295                 300 

Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
305                 310                 315                 320 

Leu Ser Leu Ser Leu Gly Lys
                325        


<210>    137
<211>    981
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG4 wild type_DNA


<400>    137
gccagcacca agggcccttc cgtgtttccc ctggcccctt gctcccggtc cacatctgag         60

agcaccgccg ccctgggctg tctggtgaag gactacttcc cagagcccgt gaccgtgagc        120

tggaacagcg gcgccctgac aagcggcgtg cacacatttc ccgccgtgct gcagagctcc        180

ggcctgtact ccctgtctag cgtggtgaca gtgccttcct ctagcctggg caccaagaca        240

tatacctgta acgtggacca caagccaagc aataccaagg tggataagcg ggtggagtct        300

aagtacggcc ctccttgccc tagctgtcct gctccagagt ttctgggcgg cccttccgtg        360

ttcctgtttc cacccaaacc aaaggacaca ctgatgatct ctagaacacc agaggtgacc        420

tgcgtggtgg tggacgtgag ccaggaggat cccgaggtgc agttcaactg gtacgtggat        480

ggcgtggagg tgcacaatgc caagaccaag ccaagagagg agcagtttaa ctctacatac        540

agggtggtga gcgtgctgac cgtgctgcac caggattggc tcaacggcaa ggagtataag        600

tgcaaggtgt ccaataaggg cctgccctcc tctatcgaga agacaatctc taaggctaag        660

ggccagccaa gagagcctca ggtgtacacc ctgcctccaa gccaggagga gatgacaaag        720

aaccaggtgt ccctgacatg tctggtgaag ggcttctatc cctccgacat cgccgtggag        780

tgggagtcta atggccagcc tgagaacaat tacaagacca caccccctgt gctggactct        840

gatggcagct tctttctgta ttccaggctg accgtggata agtctcggtg gcaggagggc        900

aacgtgttca gctgctctgt gatgcacgaa gccctgcata atcactatac tcagaaaagt        960

ctgtcactgt cactgggaaa g                                                  981


<210>    138
<211>    327
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG4 mutant TA_amino
         acid


<400>    138
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
  1               5                  10                  15 

Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
             20                  25                  30 

Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
         35                  40                  45 

Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
     50                  55                  60 

Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr
 65                  70                  75                  80 

Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
                 85                  90                  95 

Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro
            100                 105                 110 

Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
        115                 120                 125 

Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
    130                 135                 140 

Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp
145                 150                 155                 160 

Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe
                165                 170                 175 

Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
            180                 185                 190 

Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu
        195                 200                 205 

Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
    210                 215                 220 

Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
225                 230                 235                 240 

Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
                245                 250                 255 

Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
            260                 265                 270 

Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
        275                 280                 285 

Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
    290                 295                 300 

Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
305                 310                 315                 320 

Leu Ser Leu Ser Leu Gly Lys
                325        


<210>    139
<211>    981
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    heavy chain constant region(CH1, CH2, CH3)_IgG4 mutant TA_DNA


<400>    139
gccagcacca agggcccttc cgtgtttccc ctggcccctt gctcccggtc cacatctgag         60

agcaccgccg ccctgggctg tctggtgaag gactacttcc cagagcccgt gaccgtgagc        120

tggaacagcg gcgccctgac aagcggcgtg cacacatttc ccgccgtgct gcagagctcc        180

ggcctgtact ccctgtctag cgtggtgaca gtgccttcct ctagcctggg caccaagaca        240

tatacctgta acgtggacca caagccaagc aataccaagg tggataagcg ggtggagtct        300

aagtacggcc ctccttgccc tagctgtcct gctccagagt ttctgggcgg cccttccgtg        360

ttcctgtttc cacccaaacc aaaggacaca ctgatgatct ctagaacacc agaggtgacc        420

tgcgtggtgg tggacgtgag ccaggaggat cccgaggtgc agttcaactg gtacgtggat        480

ggcgtggagg tgcacaatgc caagaccaag ccaagagagg agcagtttaa ctctgcctac        540

agggtggtga gcgtgctgac cgtgctgcac caggattggc tcaacggcaa ggagtataag        600

tgcaaggtgt ccaataaggg cctgccctcc tctatcgaga agacaatctc taaggctaag        660

ggccagccaa gagagcctca ggtgtacacc ctgcctccaa gccaggagga gatgacaaag        720

aaccaggtgt ccctgacatg tctggtgaag ggcttctatc cctccgacat cgccgtggag        780

tgggagtcta atggccagcc tgagaacaat tacaagacca caccccctgt gctggactct        840

gatggcagct tctttctgta ttccaggctg accgtggata agtctcggtg gcaggagggc        900

aacgtgttca gctgctctgt gatgcacgaa gccctgcata atcactatac tcagaaaagt        960

ctgtcactgt cactgggaaa g                                                  981


<210>    140
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant TA_amino acid


<400>    140
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    141
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant TA_DNA


<400>    141
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    142
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALA_amino acid


<400>    142
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    143
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALA_DNA


<400>    143
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    144
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALATA_amino acid


<400>    144
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    145
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALATA_DNA


<400>    145
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    146
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALAPG_amino acid


<400>    146
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    147
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALAPG_DNA


<400>    147
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    148
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALAPGTA_amino acid


<400>    148
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    149
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG1 mutant LALAPGTA_DNA


<400>    149
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    150
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG4 wild type_amino acid


<400>    150
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    151
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG4 wild type_DNA


<400>    151
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    152
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG4 mutant TA_amino acid


<400>    152
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Tyr Asp Asn Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    153
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain_IgG4 mutant TA_DNA


<400>    153
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcttatg ataatggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    154
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant TA_amino acid


<400>    154
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    155
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant TA_DNA


<400>    155
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    156
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALA_amino acid


<400>    156
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    157
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALA_DNA


<400>    157
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    158
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALATA_amino acid


<400>    158
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    159
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALATA_DNA


<400>    159
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    160
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALAPG_amino acid


<400>    160
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    161
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALAPG_DNA


<400>    161
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    162
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALAPGTA_amino acid


<400>    162
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    163
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG1 mutant LALAPGTA_DNA


<400>    163
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    164
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG4 wild type_amino acid


<400>    164
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    165
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG4 wild type_DNA


<400>    165
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    166
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG4 mutant TA_amino acid


<400>    166
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Val Ile Ser Ser Asp Gly Gly Asn Thr Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    167
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 1_IgG4 mutant TA_DNA


<400>    167
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagtg atctcttctg atggtggtaa tacatattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    168
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant TA_amino acid


<400>    168
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    169
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant TA_DNA


<400>    169
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    170
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALA_amino acid


<400>    170
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    171
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALA_DNA


<400>    171
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    172
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALATA_amino acid


<400>    172
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    173
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALATA_DNA


<400>    173
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    174
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALAPG_amino acid


<400>    174
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    175
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALAPG_DNA


<400>    175
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    176
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALAPGTA_amino acid


<400>    176
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    177
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG1 mutant LALAPGTA_DNA


<400>    177
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    178
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG4 wild type_amino acid


<400>    178
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    179
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG4 wild type_DNA


<400>    179
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    180
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG4 mutant TA_amino acid


<400>    180
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Gln Met Gly Arg Val Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    181
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 2_IgG4 mutant TA_DNA


<400>    181
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctcgccgc agatgggtcg ggtgtattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    182
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant TA_amino acid


<400>    182
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    183
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant TA_DNA


<400>    183
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    184
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALA_amino acid


<400>    184
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    185
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALA_DNA


<400>    185
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    186
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALATA_amino acid


<400>    186
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    187
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALATA_DNA


<400>    187
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    188
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALAPG_amino acid


<400>    188
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    189
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALAPG_DNA


<400>    189
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    190
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALAPGTA_amino acid


<400>    190
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    191
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG1 mutant LALAPGTA_DNA


<400>    191
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    192
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG4 wild type_amino acid


<400>    192
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    193
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG4 wild type_DNA


<400>    193
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    194
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG4 mutant TA_amino acid


<400>    194
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Asp Pro Leu Gly Gly Asn Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    195
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 3_IgG4 mutant TA_DNA


<400>    195
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcgatccgt tggggggtaa tatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    196
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant TA_amino acid


<400>    196
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    197
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant TA_DNA


<400>    197
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    198
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALA_amino acid


<400>    198
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    199
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALA_DNA


<400>    199
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    200
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALATA_amino acid


<400>    200
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    201
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALATA_DNA


<400>    201
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    202
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALAPG_amino acid


<400>    202
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    203
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALAPG_DNA


<400>    203
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    204
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALAPGTA_amino acid


<400>    204
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    205
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG1 mutant LALAPGTA_DNA


<400>    205
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    206
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG4 wild type_amino acid


<400>    206
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    207
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG4 wild type_DNA


<400>    207
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    208
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG4 mutant TA_amino acid


<400>    208
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Ser
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    209
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 4_IgG4 mutant TA_DNA


<400>    209
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactctgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    210
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant TA_amino acid


<400>    210
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    211
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant TA_DNA


<400>    211
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    212
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALA_amino acid


<400>    212
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    213
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALA_DNA


<400>    213
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    214
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALATA_amino acid


<400>    214
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    215
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALATA_DNA


<400>    215
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    216
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALAPG_amino acid


<400>    216
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    217
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALAPG_DNA


<400>    217
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    218
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALAPGTA_amino acid


<400>    218
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    219
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG1 mutant LALAPGTA_DNA


<400>    219
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    220
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG4 wild type_amino acid


<400>    220
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    221
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG4 wild type_DNA


<400>    221
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    222
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG4 mutant TA_amino acid


<400>    222
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Gly Ala Asp Gly Gly Pro Ser Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    223
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 5_IgG4 mutant TA_DNA


<400>    223
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atcggggctg atgggggtcc gtcttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    224
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant TA_amino acid


<400>    224
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    225
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant TA_DNA


<400>    225
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    226
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALA_amino acid


<400>    226
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    227
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALA_DNA


<400>    227
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    228
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALATA_amino acid


<400>    228
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    229
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALATA_DNA


<400>    229
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    230
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALAPG_amino acid


<400>    230
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    231
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALAPG_DNA


<400>    231
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    232
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALAPGTA_amino acid


<400>    232
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    233
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG1 mutant LALAPGTA_DNA


<400>    233
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    234
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG4 wild type_amino acid


<400>    234
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    235
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG4 wild type_DNA


<400>    235
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    236
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG4 mutant TA_amino acid


<400>    236
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Met Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    237
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 6_IgG4 mutant TA_DNA


<400>    237
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagaatggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    238
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant TA_amino acid


<400>    238
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    239
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant TA_DNA


<400>    239
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    240
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALA_amino acid


<400>    240
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    241
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALA_DNA


<400>    241
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    242
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALATA_amino acid


<400>    242
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    243
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALATA_DNA


<400>    243
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    244
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALAPG_amino acid


<400>    244
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    245
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALAPG_DNA


<400>    245
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    246
<211>    446
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALAPGTA_amino acid


<400>    246
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr
    210                 215                 220 

Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe
225                 230                 235                 240 

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
                245                 250                 255 

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val
            260                 265                 270 

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr
        275                 280                 285 

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Ala Tyr Arg Val Val Ser Val
    290                 295                 300 

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
305                 310                 315                 320 

Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile Ser
                325                 330                 335 

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro
            340                 345                 350 

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val
        355                 360                 365 

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly
    370                 375                 380 

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp
385                 390                 395                 400 

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp
                405                 410                 415 

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His
            420                 425                 430 

Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
        435                 440                 445 




<210>    247
<211>    1338
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG1 mutant LALAPGTA_DNA


<400>    247
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc ctccaccaag        360

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc        420

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc        480

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc        540

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac        600

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac        660

aaaactcaca catgcccacc gtgcccagca cctgaagccg ccgggggacc gtcagtcttc        720

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc        780

gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc        840

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cgcctaccgt        900

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc        960

aaggtctcca acaaagccct cggcgccccc atcgagaaaa ccatctccaa agccaaaggg       1020

cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac       1080

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg       1140

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac       1200

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac       1260

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc       1320

tccctgtccc cgggtaaa                                                     1338


<210>    248
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG4 wild type_amino acid


<400>    248
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    249
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG4 wild type_DNA


<400>    249
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctacatacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329


<210>    250
<211>    443
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG4 mutant TA_amino acid


<400>    250
Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
  1               5                  10                  15 

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
             20                  25                  30 

Asp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
         35                  40                  45 

Ser Ala Ile Ser Pro Tyr Ser Gly Arg Ile Tyr Tyr Ala Asp Ser Val
     50                  55                  60 

Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr
 65                  70                  75                  80 

Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                 85                  90                  95 

Ala Arg Leu Ala Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val
            100                 105                 110 

Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala
        115                 120                 125 

Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu
    130                 135                 140 

Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly
145                 150                 155                 160 

Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser
                165                 170                 175 

Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
            180                 185                 190 

Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr
        195                 200                 205 

Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser
    210                 215                 220 

Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro
225                 230                 235                 240 

Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr
                245                 250                 255 

Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn
            260                 265                 270 

Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg
        275                 280                 285 

Glu Glu Gln Phe Asn Ser Ala Tyr Arg Val Val Ser Val Leu Thr Val
    290                 295                 300 

Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser
305                 310                 315                 320 

Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys
                325                 330                 335 

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu
            340                 345                 350 

Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe
        355                 360                 365 

Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu
    370                 375                 380 

Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
385                 390                 395                 400 

Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly
                405                 410                 415 

Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr
            420                 425                 430 

Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys
        435                 440            


<210>    251
<211>    1329
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    IgG heavy chain 7_IgG4 mutant TA_DNA


<400>    251
gaggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgagactc         60

tcctgtgcag cctctggatt cacctttagc agttatgata tgagctgggt ccgccaggct        120

ccagggaagg ggctggagtg ggtctcagcg atctctccgt attcgggtag gatttattac        180

gctgattctg taaaaggtcg gttcaccatc tccagagaca attccaagaa cacgctgtat        240

ctgcaaatga acagcctgag agccgaggac acggccgtgt attactgtgc gagactggcg        300

cttgatttcg actactgggg ccagggtaca ctggtcaccg tgagctcagc cagcaccaag        360

ggcccttccg tgtttcccct ggccccttgc tcccggtcca catctgagag caccgccgcc        420

ctgggctgtc tggtgaagga ctacttccca gagcccgtga ccgtgagctg gaacagcggc        480

gccctgacaa gcggcgtgca cacatttccc gccgtgctgc agagctccgg cctgtactcc        540

ctgtctagcg tggtgacagt gccttcctct agcctgggca ccaagacata tacctgtaac        600

gtggaccaca agccaagcaa taccaaggtg gataagcggg tggagtctaa gtacggccct        660

ccttgcccta gctgtcctgc tccagagttt ctgggcggcc cttccgtgtt cctgtttcca        720

cccaaaccaa aggacacact gatgatctct agaacaccag aggtgacctg cgtggtggtg        780

gacgtgagcc aggaggatcc cgaggtgcag ttcaactggt acgtggatgg cgtggaggtg        840

cacaatgcca agaccaagcc aagagaggag cagtttaact ctgcctacag ggtggtgagc        900

gtgctgaccg tgctgcacca ggattggctc aacggcaagg agtataagtg caaggtgtcc        960

aataagggcc tgccctcctc tatcgagaag acaatctcta aggctaaggg ccagccaaga       1020

gagcctcagg tgtacaccct gcctccaagc caggaggaga tgacaaagaa ccaggtgtcc       1080

ctgacatgtc tggtgaaggg cttctatccc tccgacatcg ccgtggagtg ggagtctaat       1140

ggccagcctg agaacaatta caagaccaca ccccctgtgc tggactctga tggcagcttc       1200

tttctgtatt ccaggctgac cgtggataag tctcggtggc aggagggcaa cgtgttcagc       1260

tgctctgtga tgcacgaagc cctgcataat cactatactc agaaaagtct gtcactgtca       1320

ctgggaaag                                                               1329
