                         SEQUENCE LISTING

<110>  KWS SAAT SE & Co. KGaA
 
<120>  USE OF GLYPHOSATE HERBICIDE FOR CONTROLLING UNWANTED VEGETATION 
       IN BETA VULGARIS GROWING AREAS

<130>  KWS0288PCT2

<150>  EP 18196615.1
<151>  2018-09-25

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  7001
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated epsps gene (P179S)

<400>  1
aggaagtatt tgaatttgat atagatattg tgtctttgtg tgtgttgaat ttcaattccc       60

agttccctaa aaaaaattta caattgcaat ttcgagatta tgatgtaaat taaatttgag      120

agactagaaa gtatttggtc aacccaaaaa aaaaatatca atacttatat aaatcaaaaa      180

cataatagag aatccaattt tactaaaaat attagtaatt ttgattaaaa taatctatta      240

aaatgaactc taaccttcac ataatttcca catattatta atcaacaaaa taagcatcac      300

aaattattag aataggcgat ctaattttaa cataaaatta gacgaattca aattgaattt      360

ttctaacaag ctcattccat ttcacgcaac ccaaaattat cctagtcagt agtcatccat      420

tcttttctca ttcctttatt cttgattatc gaactacaac agataatttc aaaaaaaaac      480

taaattggta gtcttaactg attaaactac ttactaaatg gattaaagaa tgtcattact      540

gaatagatta aactgattac gaaatagatt aacttggtcc ctaaatagat taaattagtt      600

actatattaa aattaggcga tctcttacaa aaccaactga ataagcatag ctctgtatat      660

tacctagatt tcaactaaat caaaacccct tacagttcaa tctagagctg atcattttgg      720

ctcggcccgt cccatttttg ggccgggttt tagtcagatt tttttggccc gcggtcgggc      780

ccggcccgat ttttttggct ttgggcaagc caaaaacgac ttttcagttt attttttggc      840

ccgacccgtt tttacccgca aaagcccgct aatttaggtc cgcactttgg gcacaaaaat      900

ttagcccgaa cttaaacctg gcccgaccca tgatcacctc tagtttaatc caaactaaaa      960

aactacacaa gttagccaaa aattatgtct actttgtaca actttataaa atacacacag     1020

tagttgatat cttgatgatt aactcctttt gaagtttgac tacacaccaa ccccaaacac     1080

acccactttt tcccccctct tgtcaccaac cccccctcct ctttagccac caaagtttgg     1140

ttggtgagtc ctccataact gctaaattct ctcttttttc tctctcctaa aaaactaaaa     1200

cccaccaaaa tttcagacat caaaaaaatt acaagtgaag gaaacaataa tggctcaagc     1260

tagcaccata aacaatggtg tcaaaagcac ccaattatgc cccaatttac ccaaaaccca     1320

cttatccaaa tcttcaaaat ctgttaaatt tggatcaaat ttgagatttt ctccaaagtt     1380

gaaatctttt aacaatgaaa gagttggtgg gaattcatca gttgttttca gggttagggc     1440

ttcagttgca gcagcagctg aaaaatcatc aactgtacca gaaattgtgt tacaacccat     1500

caaagagatc tcgggtacca ttcaattgcc cggatccaag tcattatcta atcggattct     1560

acttcttgct gccctttctc aggtactttt caattgtttg atttcttttt ttcttagaac     1620

ttgtgaattt gtatacttta tccgtttcta aatacgtgca acatttgaat agtaacgagt     1680

atttatctac caacttattt aatattctct cacgaatgta tatgaaaaaa tatagtcatg     1740

cgtggtttta tttgattgat ctgcggactt ttataatatc aactttttat aatttagagg     1800

acaaagtagt gtattgggta gcgtgtaagg aggttgggaa actggaggaa ttttttaaca     1860

attcaagttt gatatttttc atagtgaaat gtttattagc atagaatcat gcttttagtt     1920

tttagtggag tgtgcattta ttctttaact tgttggatgg ctatgattaa gaattggatt     1980

ctggttattt gcttgagtat ttagaaatta attgtgggtg ttggtgataa tgtaacaaaa     2040

ttgttttgaa ggtgtgagaa tgtgatttta attatatgag gaaatgatgg gttatttatt     2100

gtaatgtggg aatttatgat aatatgttgg aggatgaaac aattgatgat tttgaagtgg     2160

ggtatgaagg ctttccccca tttttttacc tttcatgtgt tgtatgtagc agactgccaa     2220

agcaattctt ctgagcagct tggcatattt ccattgcaac tcataatctc aatccaagca     2280

caatcaatta cctgccacgc tagcacttta agtagcaaaa tcgcgcttga agaaaagata     2340

ataccacagg cttctatagt tctattcctt gtgatatagg acacaagtat catcctagat     2400

gacttcccta gccctgcaaa taattcctgg aaggaagtcc gttgagtatc cttgatcaag     2460

taaaattagc gctttctttt gggtaacaca aactatactc gaaccacttt atgctaaggg     2520

atcacattgc gtctcttgca gaacttttaa catttctaac atggcataag cacggaaaca     2580

ccctgtgaag ttgctagtga ccatcttttt tgataaatca agaatccctg ttcttatttg     2640

catacaaagg gttaagtctt gtctgagagg gattgacata tctcctgctg gatagacttt     2700

gtgttcatct ctaatgttat tctgagtttg cattgtatgt gttgtgtttt cgtttttctt     2760

cttgtttgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttac ccaaacttgt acggaaattt     2820

ccatcctaac gagaattatg ctgcagggaa caacagtggt tgacaacttg ctatatagtg     2880

atgatatccg ttacatgctg gatgctttga gaactcttgg gttgaatgtg gaggatgata     2940

atatagccaa aagggcaatc gtggagggtt gcggtggttt gtttcctgtt ggaaaagatg     3000

ggaaagaaat tgaacttttt cttggaaatg caggaacagc aatgcgctca ttgacagctg     3060

cagttgctgt tgctggagga aattctaggt ttgtctacta aggccttcca tgtcatcgtg     3120

aggctgttaa tattctttta ttttgtgtgg agacttgttt cctcttgcta caattgcaaa     3180

gggggctggg attgattaac aatgcagaac tcaaacacta tctttggtat caccgtctgg     3240

caggtggcat ctgatcttgc aaaacagata tggagggttt tgccggaaag gggttggggg     3300

tgggacttgg gagtatagcc tcaatgttat agaagattgt gctatactac tgtaaaatta     3360

acttttggtt ggaaccagat atagttaata aagtattttt ttatctggtg aggggtgctt     3420

ctaggtcctg aaaagttcca ccatagaaaa aaattgaatt tcattgtgaa aagctaaggt     3480

gaattcatta aattcaatac atgttgttga ttgcttgaac tagaatactt cagatatcag     3540

ttaattgaat ttatttcttt tggagaacat gcatgaagag cttttaactc gcctctacta     3600

tcctgctctt aattattttt ataaatagat ttagcaattg ggaatatcct gtaatttgct     3660

ggttgctttt tcatatggta tatattgaat tggtgtggag gcgttttaca aagagtgaag     3720

gtaattgttg atgggtggat attgaatgga aatactttga tggcggatag catattcatc     3780

tgattggatg tctgaaattt gatgagctct agtaaatgtt cctccatgta tgcttgtgta     3840

ttgggaattt gcaatccagt agtagagaac acccctgata attgcactat tattcctgag     3900

ttgtgagagc tctcaagaag atagatgggg ttcttcaagt tttttaattg tctttaggta     3960

gtgtaaaggt tgcaatattt caaaggatct gtctgatcca acaagtctgc taaatccttc     4020

accaatctga aaggggagaa gatactgctc tctctgacct gcttgcatgc atataaagct     4080

tctaaaacaa ataggttaaa tgtaaaacaa gcgcagaagt tttcacttct cttgggaaat     4140

gtgtgccaag atctattgca tgacatgaat tcatatttat tgattatatg ctcaacatga     4200

atgggattaa gaatgaaata aagtagcatg agtaggagac aatccgcctg ctcttttaat     4260

aggcatatac aatcagtcat caacatcatt gctgctttag aatttgattt atgttgatct     4320

tggtttttgc agctatgtac ttgatggagt gccgagaatg agggagcgac ccattgggga     4380

tttggtagcg ggtctaaagc aacttggcgc cgatgttgac tgttatcttg gcacaaattg     4440

tcctcctgtt cgagtgaatg ctaaaggagg ccttcccggg ggcaaggtga attctcttgt     4500

tttgattaga gaaaaattat aagttagtgt tctatcatgc ttgacacgaa caagtttttt     4560

tatctgaaag agaaagaagt gtatgcctaa tgattaacag tcatattcaa aatgatataa     4620

aagagggtgg aaggttactt gctctatgat gatttatggt agtaccgtgt taccaatagc     4680

agaattcaag caatattctt tgaactggta ataacaagtt caagcaatca agcatagttt     4740

agattagatt ctatcaatct cttagcatag tttaaattcg cttccatcaa tctcttttgt     4800

gacgtaagaa gttctgttcc attatactta tgctttttgt gaaattttat ttgcaggtca     4860

agctctctgg atcagttagt agccaatatt tgactgcact gcttatggct actcctttgg     4920

gtcttggaga cgtggaggtt gaaatcattg ataaattgat ttctgtacca tatgtggaga     4980

tgacaataaa gctaatggaa aggtttggag tgtctgtaga gcatagtgct gactggggta     5040

ggttcttgat ccgaggtggt cagaagtaca agtaagtctc tcttttttac tacgtgtcct     5100

gaaaaatcta tgttttagta gactaagata ctataaaata catcagatct cctggaaatg     5160

cgtatgttga gggtgatgct tcaagtgcta gttacttcat aggaggggcc gcagtcactg     5220

gtgggactgt gactgttgag ggttgtggaa caagtagttt acaggtatga tttaaagcct     5280

tatttcacat ccttttactt ctctccttac tgcatctcag cttaattctg aagaactctt     5340

gttgctcaat ctctaggcat aagcttgttc tttcgacctt ctaaatttgc aaaatcattc     5400

tgttaatgta acaaacagaa gatgagatgg tgtttttgat cctgataaaa aaaattagga     5460

cattcatgat tggtttatgt tctatgacaa tttcgatgat ctcttcaatt gtaacttgag     5520

gacccttttc tgctagtaaa gattgtgtat aagctattct agactgttgt atacacctca     5580

tattccggta ttgtttatac ctaataaact tcttatgatt agtatcttac ttgtctcatg     5640

tgttttgcat agggtgatgt aaaatttgct gaagttcttg agaagatggg ttgcaaggta     5700

tcctggacag agaacagtgt cactgtcact ggaccaccca gggatgcatc tggaagaaaa     5760

cacttgcgcg ccgttgatgt caacatgaac aaaatgccag atgttgcaat gactcttgct     5820

gttgttgctc tttatgcaga tggacccacc accattagag acggtatgct taattccttt     5880

ctgtgaacat gacactcttc ttgtgccata ggtagattcc gtagttattg gtcacaatcc     5940

aaatttgcat tggtatttaa agcaagtctt aggtggtgag accgcttgaa acccataata     6000

taaaagaaag gtcaccttgg tctcaaagtt aattcttgtg agaccatctc atgcaagatt     6060

ttgccgggta tctaatcctt gtttgcactg aggaaaagga aaaaaattaa catgacctat     6120

tagtaagcca aaaaaattct aacaagagaa gtagccgaat aggtaataat ttttgtgata     6180

taatgatcag tggctagctg gagagtgaag gaaacagaac ggatgattgc gatttgcaca     6240

gagctcagaa aggttagcca ttttggatat acacattctt gaaggttttc aaccttgtag     6300

aagattgtac tattgataaa aaaaattgat gtttatccgg tgtacttcga aatttctttt     6360

cagctggggg caacagttga ggaaggatca gattactgtg tgatcactcc acctgagaaa     6420

ctaaatgtga cggccattga tacatacgat gatcaccgaa tggccatggc attctctctt     6480

gctgcctgcg ccgatgttcc tgttaccatc aaggacccgg gttgcactcg caagactttc     6540

ccagactact ttgatgtgtt ggaaaggttt gcaaagcatt aagtggtctc ctacatattc     6600

tataaagcat aagctgagat tttttgagag aattaggaga tgaaaaatgc tttctgcttg     6660

agttatcatc acattctttg tattatgatt gtaagattat tatagtatag agtttacaaa     6720

gtactactaa taattgttat gtatccgatt gatcagaaat aagttaattg gaaggctgga     6780

ctttgaaaat gtgaccaaga cactagtgtg accaagtcat tttgttaatg tgagttcaat     6840

gttattgatt caacatgtag agccaaatct caattctatc gtcacttcat atgaccaaaa     6900

atctaaagat gaaaaagtaa aaaagagcat gttggatcaa actctagctg tatctctgaa     6960

attcaatcac gcagttagat caaatgagga ttaaagggag t                         7001


<210>  2
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of mutated epsps gene (P179S)

<400>  2
atggctcaag ctagcaccat aaacaatggt gtcaaaagca cccaattatg ccccaattta       60

cccaaaaccc acttatccaa atcttcaaaa tctgttaaat ttggatcaaa tttgagattt      120

tctccaaagt tgaaatcttt taacaatgaa agagttggtg ggaattcatc agttgttttc      180

agggttaggg cttcagttgc agcagcagct gaaaaatcat caactgtacc agaaattgtg      240

ttacaaccca tcaaagagat ctcgggtacc attcaattgc ccggatccaa gtcattatct      300

aatcggattc tacttcttgc tgccctttct cagggaacaa cagtggttga caacttgcta      360

tatagtgatg atatccgtta catgctggat gctttgagaa ctcttgggtt gaatgtggag      420

gatgataata tagccaaaag ggcaatcgtg gagggttgcg gtggtttgtt tcctgttgga      480

aaagatggga aagaaattga actttttctt ggaaatgcag gaacagcaat gcgctcattg      540

acagctgcag ttgctgttgc tggaggaaat tctagctatg tacttgatgg agtgccgaga      600

atgagggagc gacccattgg ggatttggta gcgggtctaa agcaacttgg cgccgatgtt      660

gactgttatc ttggcacaaa ttgtcctcct gttcgagtga atgctaaagg aggccttccc      720

gggggcaagg tcaagctctc tggatcagtt agtagccaat atttgactgc actgcttatg      780

gctactcctt tgggtcttgg agacgtggag gttgaaatca ttgataaatt gatttctgta      840

ccatatgtgg agatgacaat aaagctaatg gaaaggtttg gagtgtctgt agagcatagt      900

gctgactggg gtaggttctt gatccgaggt ggtcagaagt acaaatctcc tggaaatgcg      960

tatgttgagg gtgatgcttc aagtgctagt tacttcatag gaggggccgc agtcactggt     1020

gggactgtga ctgttgaggg ttgtggaaca agtagtttac agggtgatgt aaaatttgct     1080

gaagttcttg agaagatggg ttgcaaggta tcctggacag agaacagtgt cactgtcact     1140

ggaccaccca gggatgcatc tggaagaaaa cacttgcgcg ccgttgatgt caacatgaac     1200

aaaatgccag atgttgcaat gactcttgct gttgttgctc tttatgcaga tggacccacc     1260

accattagag acgtggctag ctggagagtg aaggaaacag aacggatgat tgcgatttgc     1320

acagagctca gaaagctggg ggcaacagtt gaggaaggat cagattactg tgtgatcact     1380

ccacctgaga aactaaatgt gacggccatt gatacatacg atgatcaccg aatggccatg     1440

gcattctctc ttgctgcctg cgccgatgtt cctgttacca tcaaggaccc gggttgcact     1500

cgcaagactt tcccagacta ctttgatgtg ttggaaaggt ttgcaaagca ttaa           1554


<210>  3
<211>  517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein of mutated epsps gene (P179S)

<400>  3

Met Ala Gln Ala Ser Thr Ile Asn Asn Gly Val Lys Ser Thr Gln Leu 
1               5                   10                  15      


Cys Pro Asn Leu Pro Lys Thr His Leu Ser Lys Ser Ser Lys Ser Val 
            20                  25                  30          


Lys Phe Gly Ser Asn Leu Arg Phe Ser Pro Lys Leu Lys Ser Phe Asn 
        35                  40                  45              


Asn Glu Arg Val Gly Gly Asn Ser Ser Val Val Phe Arg Val Arg Ala 
    50                  55                  60                  


Ser Val Ala Ala Ala Ala Glu Lys Ser Ser Thr Val Pro Glu Ile Val 
65                  70                  75                  80  


Leu Gln Pro Ile Lys Glu Ile Ser Gly Thr Ile Gln Leu Pro Gly Ser 
                85                  90                  95      


Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Gln Gly 
            100                 105                 110         


Thr Thr Val Val Asp Asn Leu Leu Tyr Ser Asp Asp Ile Arg Tyr Met 
        115                 120                 125             


Leu Asp Ala Leu Arg Thr Leu Gly Leu Asn Val Glu Asp Asp Asn Ile 
    130                 135                 140                 


Ala Lys Arg Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Val Gly 
145                 150                 155                 160 


Lys Asp Gly Lys Glu Ile Glu Leu Phe Leu Gly Asn Ala Gly Thr Ala 
                165                 170                 175     


Met Arg Ser Leu Thr Ala Ala Val Ala Val Ala Gly Gly Asn Ser Ser 
            180                 185                 190         


Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp 
        195                 200                 205             


Leu Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Tyr Leu 
    210                 215                 220                 


Gly Thr Asn Cys Pro Pro Val Arg Val Asn Ala Lys Gly Gly Leu Pro 
225                 230                 235                 240 


Gly Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr 
                245                 250                 255     


Ala Leu Leu Met Ala Thr Pro Leu Gly Leu Gly Asp Val Glu Val Glu 
            260                 265                 270         


Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Ile Lys 
        275                 280                 285             


Leu Met Glu Arg Phe Gly Val Ser Val Glu His Ser Ala Asp Trp Gly 
    290                 295                 300                 


Arg Phe Leu Ile Arg Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala 
305                 310                 315                 320 


Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Ile Gly Gly Ala 
                325                 330                 335     


Ala Val Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly Thr Ser Ser 
            340                 345                 350         


Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Cys 
        355                 360                 365             


Lys Val Ser Trp Thr Glu Asn Ser Val Thr Val Thr Gly Pro Pro Arg 
    370                 375                 380                 


Asp Ala Ser Gly Arg Lys His Leu Arg Ala Val Asp Val Asn Met Asn 
385                 390                 395                 400 


Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Tyr Ala 
                405                 410                 415     


Asp Gly Pro Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu 
            420                 425                 430         


Thr Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala 
        435                 440                 445             


Thr Val Glu Glu Gly Ser Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys 
    450                 455                 460                 


Leu Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met 
465                 470                 475                 480 


Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Lys Asp 
                485                 490                 495     


Pro Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Asp Val Leu Glu 
            500                 505                 510         


Arg Phe Ala Lys His 
        515         


<210>  4
<211>  7001
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated epsps gene (T175I and P179S)

<400>  4
aggaagtatt tgaatttgat atagatattg tgtctttgtg tgtgttgaat ttcaattccc       60

agttccctaa aaaaaattta caattgcaat ttcgagatta tgatgtaaat taaatttgag      120

agactagaaa gtatttggtc aacccaaaaa aaaaatatca atacttatat aaatcaaaaa      180

cataatagag aatccaattt tactaaaaat attagtaatt ttgattaaaa taatctatta      240

aaatgaactc taaccttcac ataatttcca catattatta atcaacaaaa taagcatcac      300

aaattattag aataggcgat ctaattttaa cataaaatta gacgaattca aattgaattt      360

ttctaacaag ctcattccat ttcacgcaac ccaaaattat cctagtcagt agtcatccat      420

tcttttctca ttcctttatt cttgattatc gaactacaac agataatttc aaaaaaaaac      480

taaattggta gtcttaactg attaaactac ttactaaatg gattaaagaa tgtcattact      540

gaatagatta aactgattac gaaatagatt aacttggtcc ctaaatagat taaattagtt      600

actatattaa aattaggcga tctcttacaa aaccaactga ataagcatag ctctgtatat      660

tacctagatt tcaactaaat caaaacccct tacagttcaa tctagagctg atcattttgg      720

ctcggcccgt cccatttttg ggccgggttt tagtcagatt tttttggccc gcggtcgggc      780

ccggcccgat ttttttggct ttgggcaagc caaaaacgac ttttcagttt attttttggc      840

ccgacccgtt tttacccgca aaagcccgct aatttaggtc cgcactttgg gcacaaaaat      900

ttagcccgaa cttaaacctg gcccgaccca tgatcacctc tagtttaatc caaactaaaa      960

aactacacaa gttagccaaa aattatgtct actttgtaca actttataaa atacacacag     1020

tagttgatat cttgatgatt aactcctttt gaagtttgac tacacaccaa ccccaaacac     1080

acccactttt tcccccctct tgtcaccaac cccccctcct ctttagccac caaagtttgg     1140

ttggtgagtc ctccataact gctaaattct ctcttttttc tctctcctaa aaaactaaaa     1200

cccaccaaaa tttcagacat caaaaaaatt acaagtgaag gaaacaataa tggctcaagc     1260

tagcaccata aacaatggtg tcaaaagcac ccaattatgc cccaatttac ccaaaaccca     1320

cttatccaaa tcttcaaaat ctgttaaatt tggatcaaat ttgagatttt ctccaaagtt     1380

gaaatctttt aacaatgaaa gagttggtgg gaattcatca gttgttttca gggttagggc     1440

ttcagttgca gcagcagctg aaaaatcatc aactgtacca gaaattgtgt tacaacccat     1500

caaagagatc tcgggtacca ttcaattgcc cggatccaag tcattatcta atcggattct     1560

acttcttgct gccctttctc aggtactttt caattgtttg atttcttttt ttcttagaac     1620

ttgtgaattt gtatacttta tccgtttcta aatacgtgca acatttgaat agtaacgagt     1680

atttatctac caacttattt aatattctct cacgaatgta tatgaaaaaa tatagtcatg     1740

cgtggtttta tttgattgat ctgcggactt ttataatatc aactttttat aatttagagg     1800

acaaagtagt gtattgggta gcgtgtaagg aggttgggaa actggaggaa ttttttaaca     1860

attcaagttt gatatttttc atagtgaaat gtttattagc atagaatcat gcttttagtt     1920

tttagtggag tgtgcattta ttctttaact tgttggatgg ctatgattaa gaattggatt     1980

ctggttattt gcttgagtat ttagaaatta attgtgggtg ttggtgataa tgtaacaaaa     2040

ttgttttgaa ggtgtgagaa tgtgatttta attatatgag gaaatgatgg gttatttatt     2100

gtaatgtggg aatttatgat aatatgttgg aggatgaaac aattgatgat tttgaagtgg     2160

ggtatgaagg ctttccccca tttttttacc tttcatgtgt tgtatgtagc agactgccaa     2220

agcaattctt ctgagcagct tggcatattt ccattgcaac tcataatctc aatccaagca     2280

caatcaatta cctgccacgc tagcacttta agtagcaaaa tcgcgcttga agaaaagata     2340

ataccacagg cttctatagt tctattcctt gtgatatagg acacaagtat catcctagat     2400

gacttcccta gccctgcaaa taattcctgg aaggaagtcc gttgagtatc cttgatcaag     2460

taaaattagc gctttctttt gggtaacaca aactatactc gaaccacttt atgctaaggg     2520

atcacattgc gtctcttgca gaacttttaa catttctaac atggcataag cacggaaaca     2580

ccctgtgaag ttgctagtga ccatcttttt tgataaatca agaatccctg ttcttatttg     2640

catacaaagg gttaagtctt gtctgagagg gattgacata tctcctgctg gatagacttt     2700

gtgttcatct ctaatgttat tctgagtttg cattgtatgt gttgtgtttt cgtttttctt     2760

cttgtttgtg tgtgtgtgtg tgtgtgtgtg tgtgtgttac ccaaacttgt acggaaattt     2820

ccatcctaac gagaattatg ctgcagggaa caacagtggt tgacaacttg ctatatagtg     2880

atgatatccg ttacatgctg gatgctttga gaactcttgg gttgaatgtg gaggatgata     2940

atatagccaa aagggcaatc gtggagggtt gcggtggttt gtttcctgtt ggaaaagatg     3000

ggaaagaaat tgaacttttt cttggaaatg caggaatagc aatgcgctca ttgacagctg     3060

cagttgctgt tgctggagga aattctaggt ttgtctacta aggccttcca tgtcatcgtg     3120

aggctgttaa tattctttta ttttgtgtgg agacttgttt cctcttgcta caattgcaaa     3180

gggggctggg attgattaac aatgcagaac tcaaacacta tctttggtat caccgtctgg     3240

caggtggcat ctgatcttgc aaaacagata tggagggttt tgccggaaag gggttggggg     3300

tgggacttgg gagtatagcc tcaatgttat agaagattgt gctatactac tgtaaaatta     3360

acttttggtt ggaaccagat atagttaata aagtattttt ttatctggtg aggggtgctt     3420

ctaggtcctg aaaagttcca ccatagaaaa aaattgaatt tcattgtgaa aagctaaggt     3480

gaattcatta aattcaatac atgttgttga ttgcttgaac tagaatactt cagatatcag     3540

ttaattgaat ttatttcttt tggagaacat gcatgaagag cttttaactc gcctctacta     3600

tcctgctctt aattattttt ataaatagat ttagcaattg ggaatatcct gtaatttgct     3660

ggttgctttt tcatatggta tatattgaat tggtgtggag gcgttttaca aagagtgaag     3720

gtaattgttg atgggtggat attgaatgga aatactttga tggcggatag catattcatc     3780

tgattggatg tctgaaattt gatgagctct agtaaatgtt cctccatgta tgcttgtgta     3840

ttgggaattt gcaatccagt agtagagaac acccctgata attgcactat tattcctgag     3900

ttgtgagagc tctcaagaag atagatgggg ttcttcaagt tttttaattg tctttaggta     3960

gtgtaaaggt tgcaatattt caaaggatct gtctgatcca acaagtctgc taaatccttc     4020

accaatctga aaggggagaa gatactgctc tctctgacct gcttgcatgc atataaagct     4080

tctaaaacaa ataggttaaa tgtaaaacaa gcgcagaagt tttcacttct cttgggaaat     4140

gtgtgccaag atctattgca tgacatgaat tcatatttat tgattatatg ctcaacatga     4200

atgggattaa gaatgaaata aagtagcatg agtaggagac aatccgcctg ctcttttaat     4260

aggcatatac aatcagtcat caacatcatt gctgctttag aatttgattt atgttgatct     4320

tggtttttgc agctatgtac ttgatggagt gccgagaatg agggagcgac ccattgggga     4380

tttggtagcg ggtctaaagc aacttggcgc cgatgttgac tgttatcttg gcacaaattg     4440

tcctcctgtt cgagtgaatg ctaaaggagg ccttcccggg ggcaaggtga attctcttgt     4500

tttgattaga gaaaaattat aagttagtgt tctatcatgc ttgacacgaa caagtttttt     4560

tatctgaaag agaaagaagt gtatgcctaa tgattaacag tcatattcaa aatgatataa     4620

aagagggtgg aaggttactt gctctatgat gatttatggt agtaccgtgt taccaatagc     4680

agaattcaag caatattctt tgaactggta ataacaagtt caagcaatca agcatagttt     4740

agattagatt ctatcaatct cttagcatag tttaaattcg cttccatcaa tctcttttgt     4800

gacgtaagaa gttctgttcc attatactta tgctttttgt gaaattttat ttgcaggtca     4860

agctctctgg atcagttagt agccaatatt tgactgcact gcttatggct actcctttgg     4920

gtcttggaga cgtggaggtt gaaatcattg ataaattgat ttctgtacca tatgtggaga     4980

tgacaataaa gctaatggaa aggtttggag tgtctgtaga gcatagtgct gactggggta     5040

ggttcttgat ccgaggtggt cagaagtaca agtaagtctc tcttttttac tacgtgtcct     5100

gaaaaatcta tgttttagta gactaagata ctataaaata catcagatct cctggaaatg     5160

cgtatgttga gggtgatgct tcaagtgcta gttacttcat aggaggggcc gcagtcactg     5220

gtgggactgt gactgttgag ggttgtggaa caagtagttt acaggtatga tttaaagcct     5280

tatttcacat ccttttactt ctctccttac tgcatctcag cttaattctg aagaactctt     5340

gttgctcaat ctctaggcat aagcttgttc tttcgacctt ctaaatttgc aaaatcattc     5400

tgttaatgta acaaacagaa gatgagatgg tgtttttgat cctgataaaa aaaattagga     5460

cattcatgat tggtttatgt tctatgacaa tttcgatgat ctcttcaatt gtaacttgag     5520

gacccttttc tgctagtaaa gattgtgtat aagctattct agactgttgt atacacctca     5580

tattccggta ttgtttatac ctaataaact tcttatgatt agtatcttac ttgtctcatg     5640

tgttttgcat agggtgatgt aaaatttgct gaagttcttg agaagatggg ttgcaaggta     5700

tcctggacag agaacagtgt cactgtcact ggaccaccca gggatgcatc tggaagaaaa     5760

cacttgcgcg ccgttgatgt caacatgaac aaaatgccag atgttgcaat gactcttgct     5820

gttgttgctc tttatgcaga tggacccacc accattagag acggtatgct taattccttt     5880

ctgtgaacat gacactcttc ttgtgccata ggtagattcc gtagttattg gtcacaatcc     5940

aaatttgcat tggtatttaa agcaagtctt aggtggtgag accgcttgaa acccataata     6000

taaaagaaag gtcaccttgg tctcaaagtt aattcttgtg agaccatctc atgcaagatt     6060

ttgccgggta tctaatcctt gtttgcactg aggaaaagga aaaaaattaa catgacctat     6120

tagtaagcca aaaaaattct aacaagagaa gtagccgaat aggtaataat ttttgtgata     6180

taatgatcag tggctagctg gagagtgaag gaaacagaac ggatgattgc gatttgcaca     6240

gagctcagaa aggttagcca ttttggatat acacattctt gaaggttttc aaccttgtag     6300

aagattgtac tattgataaa aaaaattgat gtttatccgg tgtacttcga aatttctttt     6360

cagctggggg caacagttga ggaaggatca gattactgtg tgatcactcc acctgagaaa     6420

ctaaatgtga cggccattga tacatacgat gatcaccgaa tggccatggc attctctctt     6480

gctgcctgcg ccgatgttcc tgttaccatc aaggacccgg gttgcactcg caagactttc     6540

ccagactact ttgatgtgtt ggaaaggttt gcaaagcatt aagtggtctc ctacatattc     6600

tataaagcat aagctgagat tttttgagag aattaggaga tgaaaaatgc tttctgcttg     6660

agttatcatc acattctttg tattatgatt gtaagattat tatagtatag agtttacaaa     6720

gtactactaa taattgttat gtatccgatt gatcagaaat aagttaattg gaaggctgga     6780

ctttgaaaat gtgaccaaga cactagtgtg accaagtcat tttgttaatg tgagttcaat     6840

gttattgatt caacatgtag agccaaatct caattctatc gtcacttcat atgaccaaaa     6900

atctaaagat gaaaaagtaa aaaagagcat gttggatcaa actctagctg tatctctgaa     6960

attcaatcac gcagttagat caaatgagga ttaaagggag t                         7001


<210>  5
<211>  1554
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of mutated epsps gene (T175I and P179S)

<400>  5
atggctcaag ctagcaccat aaacaatggt gtcaaaagca cccaattatg ccccaattta       60

cccaaaaccc acttatccaa atcttcaaaa tctgttaaat ttggatcaaa tttgagattt      120

tctccaaagt tgaaatcttt taacaatgaa agagttggtg ggaattcatc agttgttttc      180

agggttaggg cttcagttgc agcagcagct gaaaaatcat caactgtacc agaaattgtg      240

ttacaaccca tcaaagagat ctcgggtacc attcaattgc ccggatccaa gtcattatct      300

aatcggattc tacttcttgc tgccctttct cagggaacaa cagtggttga caacttgcta      360

tatagtgatg atatccgtta catgctggat gctttgagaa ctcttgggtt gaatgtggag      420

gatgataata tagccaaaag ggcaatcgtg gagggttgcg gtggtttgtt tcctgttgga      480

aaagatggga aagaaattga actttttctt ggaaatgcag gaatagcaat gcgctcattg      540

acagctgcag ttgctgttgc tggaggaaat tctagctatg tacttgatgg agtgccgaga      600

atgagggagc gacccattgg ggatttggta gcgggtctaa agcaacttgg cgccgatgtt      660

gactgttatc ttggcacaaa ttgtcctcct gttcgagtga atgctaaagg aggccttccc      720

gggggcaagg tcaagctctc tggatcagtt agtagccaat atttgactgc actgcttatg      780

gctactcctt tgggtcttgg agacgtggag gttgaaatca ttgataaatt gatttctgta      840

ccatatgtgg agatgacaat aaagctaatg gaaaggtttg gagtgtctgt agagcatagt      900

gctgactggg gtaggttctt gatccgaggt ggtcagaagt acaaatctcc tggaaatgcg      960

tatgttgagg gtgatgcttc aagtgctagt tacttcatag gaggggccgc agtcactggt     1020

gggactgtga ctgttgaggg ttgtggaaca agtagtttac agggtgatgt aaaatttgct     1080

gaagttcttg agaagatggg ttgcaaggta tcctggacag agaacagtgt cactgtcact     1140

ggaccaccca gggatgcatc tggaagaaaa cacttgcgcg ccgttgatgt caacatgaac     1200

aaaatgccag atgttgcaat gactcttgct gttgttgctc tttatgcaga tggacccacc     1260

accattagag acgtggctag ctggagagtg aaggaaacag aacggatgat tgcgatttgc     1320

acagagctca gaaagctggg ggcaacagtt gaggaaggat cagattactg tgtgatcact     1380

ccacctgaga aactaaatgt gacggccatt gatacatacg atgatcaccg aatggccatg     1440

gcattctctc ttgctgcctg cgccgatgtt cctgttacca tcaaggaccc gggttgcact     1500

cgcaagactt tcccagacta ctttgatgtg ttggaaaggt ttgcaaagca ttaa           1554


<210>  6
<211>  517
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein of mutated epsps gene (T175I and P179S)

<400>  6

Met Ala Gln Ala Ser Thr Ile Asn Asn Gly Val Lys Ser Thr Gln Leu 
1               5                   10                  15      


Cys Pro Asn Leu Pro Lys Thr His Leu Ser Lys Ser Ser Lys Ser Val 
            20                  25                  30          


Lys Phe Gly Ser Asn Leu Arg Phe Ser Pro Lys Leu Lys Ser Phe Asn 
        35                  40                  45              


Asn Glu Arg Val Gly Gly Asn Ser Ser Val Val Phe Arg Val Arg Ala 
    50                  55                  60                  


Ser Val Ala Ala Ala Ala Glu Lys Ser Ser Thr Val Pro Glu Ile Val 
65                  70                  75                  80  


Leu Gln Pro Ile Lys Glu Ile Ser Gly Thr Ile Gln Leu Pro Gly Ser 
                85                  90                  95      


Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Gln Gly 
            100                 105                 110         


Thr Thr Val Val Asp Asn Leu Leu Tyr Ser Asp Asp Ile Arg Tyr Met 
        115                 120                 125             


Leu Asp Ala Leu Arg Thr Leu Gly Leu Asn Val Glu Asp Asp Asn Ile 
    130                 135                 140                 


Ala Lys Arg Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Val Gly 
145                 150                 155                 160 


Lys Asp Gly Lys Glu Ile Glu Leu Phe Leu Gly Asn Ala Gly Ile Ala 
                165                 170                 175     


Met Arg Ser Leu Thr Ala Ala Val Ala Val Ala Gly Gly Asn Ser Ser 
            180                 185                 190         


Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp 
        195                 200                 205             


Leu Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Tyr Leu 
    210                 215                 220                 


Gly Thr Asn Cys Pro Pro Val Arg Val Asn Ala Lys Gly Gly Leu Pro 
225                 230                 235                 240 


Gly Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr 
                245                 250                 255     


Ala Leu Leu Met Ala Thr Pro Leu Gly Leu Gly Asp Val Glu Val Glu 
            260                 265                 270         


Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Ile Lys 
        275                 280                 285             


Leu Met Glu Arg Phe Gly Val Ser Val Glu His Ser Ala Asp Trp Gly 
    290                 295                 300                 


Arg Phe Leu Ile Arg Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala 
305                 310                 315                 320 


Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Ile Gly Gly Ala 
                325                 330                 335     


Ala Val Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly Thr Ser Ser 
            340                 345                 350         


Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Cys 
        355                 360                 365             


Lys Val Ser Trp Thr Glu Asn Ser Val Thr Val Thr Gly Pro Pro Arg 
    370                 375                 380                 


Asp Ala Ser Gly Arg Lys His Leu Arg Ala Val Asp Val Asn Met Asn 
385                 390                 395                 400 


Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Tyr Ala 
                405                 410                 415     


Asp Gly Pro Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu 
            420                 425                 430         


Thr Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala 
        435                 440                 445             


Thr Val Glu Glu Gly Ser Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys 
    450                 455                 460                 


Leu Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met 
465                 470                 475                 480 


Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Lys Asp 
                485                 490                 495     


Pro Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Asp Val Leu Glu 
            500                 505                 510         


Arg Phe Ala Lys His 
        515         


<210>  7
<211>  4501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated als gene (W569L)

<400>  7
cgtggtaagg tttttcttcc tattgggcct aggaagtttt ccagccttgt aaaaatcttg       60

tgtgcttttt ctctttcgtt ttttagttat tttcattccg caatctaaat tcgaaaattt      120

tccctcaact acaattcaac cccttcttgt atttggtcta gtgttcatac tagaataaca      180

caaaatcgtg attaaatcta tgtattggat tgatagagaa acataaactc tttgaagaaa      240

cctaattatg ttaggacttc taatagtttc cgtcacgttt tcatttgtat tagaatttta      300

gaggtttaac tactataaag aattcatgtt ataatggaac ttgagtaata caagtcctgt      360

aagatgagaa gactaatcat gttagaagta taatcatgat agaagtctaa ttttattaat      420

agtcttttaa tgttagaatt ctaaagtttt taggagctta attaaggtag ttttcctacc      480

tatataagat tcctagtttc ctattacttg taataggatt cttagtttta taattatgaa      540

ataatttcta atcctaattg ttttcttata aataggctac ggctggtcac ctacaccaat      600

aaaattcaaa agtttactga ttaaatttga tagtttttct tcttatatga tctataagac      660

tagcttaatt agaatattaa gatttccccc ttattccacc ttcaaattag tgtacataac      720

tttctccatt aaaaatttca agaaaccttt tcctaattaa accatatatt ctaaaactcc      780

attaatagga acccctcgtt cctcatcaat tttttttttt taaaaggctt ttttttcttc      840

aacccatcat atccacattt acaagagcag ggtattttgg taagtttcca tatatagaaa      900

gtggaatcga gcgcctccac tcatttcctc ctcaaaagaa caagaacaag aacgagaaca      960

agaacaacca tcctcattct ctctccaaaa ctccaaacaa caacaatggc ggctaccttc     1020

acaaacccaa cattttcccc ttcctcaact ccattaacca aaaccctaaa atcccaatct     1080

tccatctctt caaccctccc cttttccacc cctcccaaaa ccccaactcc actctttcac     1140

cgtcccctcc aaatctcatc ctcccaatcc cacaaatcat ccgccattaa aacacaaact     1200

caagcacctt cttctccagc tattgaagat tcatctttcg tttctcgatt tggccctgat     1260

gaacccagaa aagggtccga tgtcctcgtt gaagctcttg agcgtgaagg tgttaccaat     1320

gtgtttgctt accctggtgg tgcatctatg gaaatccacc aagctctcac acgctctaaa     1380

accatccgca atgtcctccc tcgccatgaa caaggcgggg ttttcgccgc cgagggatat     1440

gctagagcta ctggaaaggt tggtgtctgc attgcgactt ctggtcctgg tgctaccaac     1500

ctcgtatcag gtcttgctga cgctctcctt gattctgtcc ctcttgttgc catcactggc     1560

caagttccac gccgtatgat tggcactgat gcttttcagg agactccaat tgttgaggtg     1620

acaaggtcta ttactaagca taattattta gttttggatg tagaggatat tcctagaatt     1680

gttaaggaag cctttttttt agctaattct ggtaggcctg gacctgtttt gattgatctt     1740

cctaaagata ttcagcagca attggttgtt cctgattggg ataggccttt taagttgggt     1800

gggtatatgt ctaggctgcc aaagtccaag ttttcgacga atgaggttgg acttcttgag     1860

cagattgtga ggttgatgag tgagtcgaag aagcctgtct tgtatgtggg aggtgggtgt     1920

ttgaattcta gtgaggagtt gaggagattt gttgagttga cagggattcc ggtggctagt     1980

actttgatgg ggttggggtc ttacccttgt aatgatgaac tgtctcttca tatgttgggg     2040

atgcacggga ctgtttatgc caattatgcg gtggataagg cggatttgtt gcttgctttc     2100

ggggttaggt ttgatgatcg tgtgaccggg aagctcgagg cgtttgctag ccgtgctaag     2160

attgtgcata ttgatattga ctctgctgag attgggaaga acaagcagcc ccatgtgtcc     2220

atttgtgctg atgttaaatt ggcattgcgg ggtatgaata agattctgga gtctagaata     2280

gggaagctga atttggattt ctccaagtgg agagaagaat taggtgagca gaagaaggaa     2340

ttcccactga gttttaagac atttggggat gcaattcctc cacaatatgc cattcaggtg     2400

cttgatgagt tgaccaatgg taatgctatt ataagtactg gtgttgggca gcaccaaatg     2460

tgggctgcgc agcattacaa gtacagaaac cctcgccaat ggctgacctc tggtgggttg     2520

ggggctatgg ggtttgggct accagccgcc attggagctg cagttgctcg accagatgca     2580

gtggttgtcg atattgatgg ggatggcagt tttattatga atgttcaaga gttggctaca     2640

attagggtgg aaaatctccc agttaagata atgctgctaa acaatcaaca tttaggtatg     2700

gttgtccaat tggaagatag gttctataaa gctaaccggg cacatacata ccttggaaac     2760

ccttccaaat ctgctgatat cttccctgat atgctcaaat tcgctgaggc atgtgatatt     2820

ccttctgccc gtgttagcaa cgtggctgat ttgagggccg ccattcaaac aatgttggat     2880

actccagggc cgtacctgct cgatgtgatt gtaccgcatc aagagcatgt gttgcctatg     2940

attccaagtg gtgccggttt caaggatacc attacagagg gtgatggaag aacctcttat     3000

tgatcggttt aatgacggtt ggaaccattt aaagagggta agctatatta ctgtatgtat     3060

attagtatgt tcctggataa tttagaagct tttgtctgtt gtcttttgca gtttatgaag     3120

ttagtttgct gttgtcatgt tacttgttac tttaaaaagc tttttgtagt ttttgagcaa     3180

ctagtatgga atgctcttcc tgtattgctt ggaaaattca caaaagtggt ttttcggcta     3240

tggatgttgt gttgcatcat gcatatatag cttgatatac tagttggctt ggtgcatctt     3300

taacatatac taatgagact acgacagcaa ttgccaatta gttggcttga cataattctt     3360

agtctgccac tagaaatctt gcttcttttt tttccctcat ttgttgaaag tccctgttgc     3420

gacctgacat gggagcattg gagattgttt agcaatagca gttgcagtta ggtgactaca     3480

gtcatcttcc aaatatgaat actctctgga ggggaggagg ttttacaaaa tatgagtttt     3540

taacacatga gaaacttata ttaaacaagg ttgagtcacc ccatattttt caaagttgac     3600

ttttgtctga tttgggtgac tatgcctgtt gtatgcaata aactcgatgt acataagact     3660

tgtataatcc aatctaaccc ttcctggctg attatgaaac cgagtcggct aatttgttgc     3720

ttgatcttca tgtgtgagcc tgatgccagg tgaccactag aggagtacct ttcattgaga     3780

tatcgattcg gttaattggt tcctcatatg ggtctcaaaa ctgaattttt ctcaggctct     3840

cttctaacca gttgttgaat tttatgaaac ttcagtccag ttaaaacttt gatcccggat     3900

cagaatttct ctgagttgtt cttccgcctc tcagttccac tgttccagtg ttcttggtcc     3960

tcaacctgta ctctgagcta tttctgcaaa cacctgaagt tcctgctgcc cttggacaaa     4020

tacagaatgc agcattagca tttatttaag acaagatgaa tacttgctcc cctgttagtt     4080

atgctaattc tgcattagta ttctttaatt ttaattagat actgcaatcc gtgaactctg     4140

cagtttcttg atctctctct gtttcaattt ctcctatctt atcgcccatt tctttaggct     4200

ttcgttatct atcgtctaat tcaagagtag gtaactaggt acgggataaa actttttcat     4260

gaagaaccta tgttcccgta ttatgctgtc ccacaagctt caactttcta ccttgttttc     4320

tatacgttgg acaacttctt ttgtgttgaa ctgatttaat tgataaatga ataattttgg     4380

ataaagaaaa ttgattgacc aataatttat ttattttagt ttacgttttg tatataccga     4440

ctgggctcat gtgagcacat ttatgtgcac aatttttttt atgtgaaaac aaaactaagc     4500

t                                                                     4501


<210>  8
<211>  1998
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of mutated als gene (W569L)

<400>  8
atggcggcta ccttcacaaa cccaacattt tccccttcct caactccatt aaccaaaacc       60

ctaaaatccc aatcttccat ctcttcaacc ctcccctttt ccacccctcc caaaacccca      120

actccactct ttcaccgtcc cctccaaatc tcatcctccc aatcccacaa atcatccgcc      180

attaaaacac aaactcaagc accttcttct ccagctattg aagattcatc tttcgtttct      240

cgatttggcc ctgatgaacc cagaaaaggg tccgatgtcc tcgttgaagc tcttgagcgt      300

gaaggtgtta ccaatgtgtt tgcttaccct ggtggtgcat ctatggaaat ccaccaagct      360

ctcacacgct ctaaaaccat ccgcaatgtc ctccctcgcc atgaacaagg cggggttttc      420

gccgccgagg gatatgctag agctactgga aaggttggtg tctgcattgc gacttctggt      480

cctggtgcta ccaacctcgt atcaggtctt gctgacgctc tccttgattc tgtccctctt      540

gttgccatca ctggccaagt tccacgccgt atgattggca ctgatgcttt tcaggagact      600

ccaattgttg aggtgacaag gtctattact aagcataatt atttagtttt ggatgtagag      660

gatattccta gaattgttaa ggaagccttt tttttagcta attctggtag gcctggacct      720

gttttgattg atcttcctaa agatattcag cagcaattgg ttgttcctga ttgggatagg      780

ccttttaagt tgggtgggta tatgtctagg ctgccaaagt ccaagttttc gacgaatgag      840

gttggacttc ttgagcagat tgtgaggttg atgagtgagt cgaagaagcc tgtcttgtat      900

gtgggaggtg ggtgtttgaa ttctagtgag gagttgagga gatttgttga gttgacaggg      960

attccggtgg ctagtacttt gatggggttg gggtcttacc cttgtaatga tgaactgtct     1020

cttcatatgt tggggatgca cgggactgtt tatgccaatt atgcggtgga taaggcggat     1080

ttgttgcttg ctttcggggt taggtttgat gatcgtgtga ccgggaagct cgaggcgttt     1140

gctagccgtg ctaagattgt gcatattgat attgactctg ctgagattgg gaagaacaag     1200

cagccccatg tgtccatttg tgctgatgtt aaattggcat tgcggggtat gaataagatt     1260

ctggagtcta gaatagggaa gctgaatttg gatttctcca agtggagaga agaattaggt     1320

gagcagaaga aggaattccc actgagtttt aagacatttg gggatgcaat tcctccacaa     1380

tatgccattc aggtgcttga tgagttgacc aatggtaatg ctattataag tactggtgtt     1440

gggcagcacc aaatgtgggc tgcgcagcat tacaagtaca gaaaccctcg ccaatggctg     1500

acctctggtg ggttgggggc tatggggttt gggctaccag ccgccattgg agctgcagtt     1560

gctcgaccag atgcagtggt tgtcgatatt gatggggatg gcagttttat tatgaatgtt     1620

caagagttgg ctacaattag ggtggaaaat ctcccagtta agataatgct gctaaacaat     1680

caacatttag gtatggttgt ccaattggaa gataggttct ataaagctaa ccgggcacat     1740

acataccttg gaaacccttc caaatctgct gatatcttcc ctgatatgct caaattcgct     1800

gaggcatgtg atattccttc tgcccgtgtt agcaacgtgg ctgatttgag ggccgccatt     1860

caaacaatgt tggatactcc agggccgtac ctgctcgatg tgattgtacc gcatcaagag     1920

catgtgttgc ctatgattcc aagtggtgcc ggtttcaagg ataccattac agagggtgat     1980

ggaagaacct cttattga                                                   1998


<210>  9
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein of mutated als gene (W569L)

<400>  9

Met Ala Ala Thr Phe Thr Asn Pro Thr Phe Ser Pro Ser Ser Thr Pro 
1               5                   10                  15      


Leu Thr Lys Thr Leu Lys Ser Gln Ser Ser Ile Ser Ser Thr Leu Pro 
            20                  25                  30          


Phe Ser Thr Pro Pro Lys Thr Pro Thr Pro Leu Phe His Arg Pro Leu 
        35                  40                  45              


Gln Ile Ser Ser Ser Gln Ser His Lys Ser Ser Ala Ile Lys Thr Gln 
    50                  55                  60                  


Thr Gln Ala Pro Ser Ser Pro Ala Ile Glu Asp Ser Ser Phe Val Ser 
65                  70                  75                  80  


Arg Phe Gly Pro Asp Glu Pro Arg Lys Gly Ser Asp Val Leu Val Glu 
                85                  90                  95      


Ala Leu Glu Arg Glu Gly Val Thr Asn Val Phe Ala Tyr Pro Gly Gly 
            100                 105                 110         


Ala Ser Met Glu Ile His Gln Ala Leu Thr Arg Ser Lys Thr Ile Arg 
        115                 120                 125             


Asn Val Leu Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly 
    130                 135                 140                 


Tyr Ala Arg Ala Thr Gly Lys Val Gly Val Cys Ile Ala Thr Ser Gly 
145                 150                 155                 160 


Pro Gly Ala Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp 
                165                 170                 175     


Ser Val Pro Leu Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile 
            180                 185                 190         


Gly Thr Asp Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser 
        195                 200                 205             


Ile Thr Lys His Asn Tyr Leu Val Leu Asp Val Glu Asp Ile Pro Arg 
    210                 215                 220                 


Ile Val Lys Glu Ala Phe Phe Leu Ala Asn Ser Gly Arg Pro Gly Pro 
225                 230                 235                 240 


Val Leu Ile Asp Leu Pro Lys Asp Ile Gln Gln Gln Leu Val Val Pro 
                245                 250                 255     


Asp Trp Asp Arg Pro Phe Lys Leu Gly Gly Tyr Met Ser Arg Leu Pro 
            260                 265                 270         


Lys Ser Lys Phe Ser Thr Asn Glu Val Gly Leu Leu Glu Gln Ile Val 
        275                 280                 285             


Arg Leu Met Ser Glu Ser Lys Lys Pro Val Leu Tyr Val Gly Gly Gly 
    290                 295                 300                 


Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly 
305                 310                 315                 320 


Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser Tyr Pro Cys Asn 
                325                 330                 335     


Asp Glu Leu Ser Leu His Met Leu Gly Met His Gly Thr Val Tyr Ala 
            340                 345                 350         


Asn Tyr Ala Val Asp Lys Ala Asp Leu Leu Leu Ala Phe Gly Val Arg 
        355                 360                 365             


Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala Phe Ala Ser Arg Ala 
    370                 375                 380                 


Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu Ile Gly Lys Asn Lys 
385                 390                 395                 400 


Gln Pro His Val Ser Ile Cys Ala Asp Val Lys Leu Ala Leu Arg Gly 
                405                 410                 415     


Met Asn Lys Ile Leu Glu Ser Arg Ile Gly Lys Leu Asn Leu Asp Phe 
            420                 425                 430         


Ser Lys Trp Arg Glu Glu Leu Gly Glu Gln Lys Lys Glu Phe Pro Leu 
        435                 440                 445             


Ser Phe Lys Thr Phe Gly Asp Ala Ile Pro Pro Gln Tyr Ala Ile Gln 
    450                 455                 460                 


Val Leu Asp Glu Leu Thr Asn Gly Asn Ala Ile Ile Ser Thr Gly Val 
465                 470                 475                 480 


Gly Gln His Gln Met Trp Ala Ala Gln His Tyr Lys Tyr Arg Asn Pro 
                485                 490                 495     


Arg Gln Trp Leu Thr Ser Gly Gly Leu Gly Ala Met Gly Phe Gly Leu 
            500                 505                 510         


Pro Ala Ala Ile Gly Ala Ala Val Ala Arg Pro Asp Ala Val Val Val 
        515                 520                 525             


Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn Val Gln Glu Leu Ala 
    530                 535                 540                 


Thr Ile Arg Val Glu Asn Leu Pro Val Lys Ile Met Leu Leu Asn Asn 
545                 550                 555                 560 


Gln His Leu Gly Met Val Val Gln Leu Glu Asp Arg Phe Tyr Lys Ala 
                565                 570                 575     


Asn Arg Ala His Thr Tyr Leu Gly Asn Pro Ser Lys Ser Ala Asp Ile 
            580                 585                 590         


Phe Pro Asp Met Leu Lys Phe Ala Glu Ala Cys Asp Ile Pro Ser Ala 
        595                 600                 605             


Arg Val Ser Asn Val Ala Asp Leu Arg Ala Ala Ile Gln Thr Met Leu 
    610                 615                 620                 


Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile Val Pro His Gln Glu 
625                 630                 635                 640 


His Val Leu Pro Met Ile Pro Ser Gly Ala Gly Phe Lys Asp Thr Ile 
                645                 650                 655     


Thr Glu Gly Asp Gly Arg Thr Ser Tyr 
            660                 665 


<210>  10
<211>  4501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of mutated als gene (P188S and W569L)

<400>  10
cgtggtaagg tttttcttcc tattgggcct aggaagtttt ccagccttgt aaaaatcttg       60

tgtgcttttt ctctttcgtt ttttagttat tttcattccg caatctaaat tcgaaaattt      120

tccctcaact acaattcaac cccttcttgt atttggtcta gtgttcatac tagaataaca      180

caaaatcgtg attaaatcta tgtattggat tgatagagaa acataaactc tttgaagaaa      240

cctaattatg ttaggacttc taatagtttc cgtcacgttt tcatttgtat tagaatttta      300

gaggtttaac tactataaag aattcatgtt ataatggaac ttgagtaata caagtcctgt      360

aagatgagaa gactaatcat gttagaagta taatcatgat agaagtctaa ttttattaat      420

agtcttttaa tgttagaatt ctaaagtttt taggagctta attaaggtag ttttcctacc      480

tatataagat tcctagtttc ctattacttg taataggatt cttagtttta taattatgaa      540

ataatttcta atcctaattg ttttcttata aataggctac ggctggtcac ctacaccaat      600

aaaattcaaa agtttactga ttaaatttga tagtttttct tcttatatga tctataagac      660

tagcttaatt agaatattaa gatttccccc ttattccacc ttcaaattag tgtacataac      720

tttctccatt aaaaatttca agaaaccttt tcctaattaa accatatatt ctaaaactcc      780

attaatagga acccctcgtt cctcatcaat tttttttttt taaaaggctt ttttttcttc      840

aacccatcat atccacattt acaagagcag ggtattttgg taagtttcca tatatagaaa      900

gtggaatcga gcgcctccac tcatttcctc ctcaaaagaa caagaacaag aacgagaaca      960

agaacaacca tcctcattct ctctccaaaa ctccaaacaa caacaatggc ggctaccttc     1020

acaaacccaa cattttcccc ttcctcaact ccattaacca aaaccctaaa atcccaatct     1080

tccatctctt caaccctccc cttttccacc cctcccaaaa ccccaactcc actctttcac     1140

cgtcccctcc aaatctcatc ctcccaatcc cacaaatcat ccgccattaa aacacaaact     1200

caagcacctt cttctccagc tattgaagat tcatctttcg tttctcgatt tggccctgat     1260

gaacccagaa aagggtccga tgtcctcgtt gaagctcttg agcgtgaagg tgttaccaat     1320

gtgtttgctt accctggtgg tgcatctatg gaaatccacc aagctctcac acgctctaaa     1380

accatccgca atgtcctccc tcgccatgaa caaggcgggg ttttcgccgc cgagggatat     1440

gctagagcta ctggaaaggt tggtgtctgc attgcgactt ctggtcctgg tgctaccaac     1500

ctcgtatcag gtcttgctga cgctctcctt gattctgtcc ctcttgttgc catcactggc     1560

caagtttcac gccgtatgat tggcactgat gcttttcagg agactccaat tgttgaggtg     1620

acaaggtcta ttactaagca taattattta gttttggatg tagaggatat tcctagaatt     1680

gttaaggaag cctttttttt agctaattct ggtaggcctg gacctgtttt gattgatctt     1740

cctaaagata ttcagcagca attggttgtt cctgattggg ataggccttt taagttgggt     1800

gggtatatgt ctaggctgcc aaagtccaag ttttcgacga atgaggttgg acttcttgag     1860

cagattgtga ggttgatgag tgagtcgaag aagcctgtct tgtatgtggg aggtgggtgt     1920

ttgaattcta gtgaggagtt gaggagattt gttgagttga cagggattcc ggtggctagt     1980

actttgatgg ggttggggtc ttacccttgt aatgatgaac tgtctcttca tatgttgggg     2040

atgcacggga ctgtttatgc caattatgcg gtggataagg cggatttgtt gcttgctttc     2100

ggggttaggt ttgatgatcg tgtgaccggg aagctcgagg cgtttgctag ccgtgctaag     2160

attgtgcata ttgatattga ctctgctgag attgggaaga acaagcagcc ccatgtgtcc     2220

atttgtgctg atgttaaatt ggcattgcgg ggtatgaata agattctgga gtctagaata     2280

gggaagctga atttggattt ctccaagtgg agagaagaat taggtgagca gaagaaggaa     2340

ttcccactga gttttaagac atttggggat gcaattcctc cacaatatgc cattcaggtg     2400

cttgatgagt tgaccaatgg taatgctatt ataagtactg gtgttgggca gcaccaaatg     2460

tgggctgcgc agcattacaa gtacagaaac cctcgccaat ggctgacctc tggtgggttg     2520

ggggctatgg ggtttgggct accagccgcc attggagctg cagttgctcg accagatgca     2580

gtggttgtcg atattgatgg ggatggcagt tttattatga atgttcaaga gttggctaca     2640

attagggtgg aaaatctccc agttaagata atgctgctaa acaatcaaca tttaggtatg     2700

gttgtccaat tggaagatag gttctataaa gctaaccggg cacatacata ccttggaaac     2760

ccttccaaat ctgctgatat cttccctgat atgctcaaat tcgctgaggc atgtgatatt     2820

ccttctgccc gtgttagcaa cgtggctgat ttgagggccg ccattcaaac aatgttggat     2880

actccagggc cgtacctgct cgatgtgatt gtaccgcatc aagagcatgt gttgcctatg     2940

attccaagtg gtgccggttt caaggatacc attacagagg gtgatggaag aacctcttat     3000

tgatcggttt aatgacggtt ggaaccattt aaagagggta agctatatta ctgtatgtat     3060

attagtatgt tcctggataa tttagaagct tttgtctgtt gtcttttgca gtttatgaag     3120

ttagtttgct gttgtcatgt tacttgttac tttaaaaagc tttttgtagt ttttgagcaa     3180

ctagtatgga atgctcttcc tgtattgctt ggaaaattca caaaagtggt ttttcggcta     3240

tggatgttgt gttgcatcat gcatatatag cttgatatac tagttggctt ggtgcatctt     3300

taacatatac taatgagact acgacagcaa ttgccaatta gttggcttga cataattctt     3360

agtctgccac tagaaatctt gcttcttttt tttccctcat ttgttgaaag tccctgttgc     3420

gacctgacat gggagcattg gagattgttt agcaatagca gttgcagtta ggtgactaca     3480

gtcatcttcc aaatatgaat actctctgga ggggaggagg ttttacaaaa tatgagtttt     3540

taacacatga gaaacttata ttaaacaagg ttgagtcacc ccatattttt caaagttgac     3600

ttttgtctga tttgggtgac tatgcctgtt gtatgcaata aactcgatgt acataagact     3660

tgtataatcc aatctaaccc ttcctggctg attatgaaac cgagtcggct aatttgttgc     3720

ttgatcttca tgtgtgagcc tgatgccagg tgaccactag aggagtacct ttcattgaga     3780

tatcgattcg gttaattggt tcctcatatg ggtctcaaaa ctgaattttt ctcaggctct     3840

cttctaacca gttgttgaat tttatgaaac ttcagtccag ttaaaacttt gatcccggat     3900

cagaatttct ctgagttgtt cttccgcctc tcagttccac tgttccagtg ttcttggtcc     3960

tcaacctgta ctctgagcta tttctgcaaa cacctgaagt tcctgctgcc cttggacaaa     4020

tacagaatgc agcattagca tttatttaag acaagatgaa tacttgctcc cctgttagtt     4080

atgctaattc tgcattagta ttctttaatt ttaattagat actgcaatcc gtgaactctg     4140

cagtttcttg atctctctct gtttcaattt ctcctatctt atcgcccatt tctttaggct     4200

ttcgttatct atcgtctaat tcaagagtag gtaactaggt acgggataaa actttttcat     4260

gaagaaccta tgttcccgta ttatgctgtc ccacaagctt caactttcta ccttgttttc     4320

tatacgttgg acaacttctt ttgtgttgaa ctgatttaat tgataaatga ataattttgg     4380

ataaagaaaa ttgattgacc aataatttat ttattttagt ttacgttttg tatataccga     4440

ctgggctcat gtgagcacat ttatgtgcac aatttttttt atgtgaaaac aaaactaagc     4500

t                                                                     4501


<210>  11
<211>  1998
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of mutated als gene (P188S and W569L)

<400>  11
atggcggcta ccttcacaaa cccaacattt tccccttcct caactccatt aaccaaaacc       60

ctaaaatccc aatcttccat ctcttcaacc ctcccctttt ccacccctcc caaaacccca      120

actccactct ttcaccgtcc cctccaaatc tcatcctccc aatcccacaa atcatccgcc      180

attaaaacac aaactcaagc accttcttct ccagctattg aagattcatc tttcgtttct      240

cgatttggcc ctgatgaacc cagaaaaggg tccgatgtcc tcgttgaagc tcttgagcgt      300

gaaggtgtta ccaatgtgtt tgcttaccct ggtggtgcat ctatggaaat ccaccaagct      360

ctcacacgct ctaaaaccat ccgcaatgtc ctccctcgcc atgaacaagg cggggttttc      420

gccgccgagg gatatgctag agctactgga aaggttggtg tctgcattgc gacttctggt      480

cctggtgcta ccaacctcgt atcaggtctt gctgacgctc tccttgattc tgtccctctt      540

gttgccatca ctggccaagt ttcacgccgt atgattggca ctgatgcttt tcaggagact      600

ccaattgttg aggtgacaag gtctattact aagcataatt atttagtttt ggatgtagag      660

gatattccta gaattgttaa ggaagccttt tttttagcta attctggtag gcctggacct      720

gttttgattg atcttcctaa agatattcag cagcaattgg ttgttcctga ttgggatagg      780

ccttttaagt tgggtgggta tatgtctagg ctgccaaagt ccaagttttc gacgaatgag      840

gttggacttc ttgagcagat tgtgaggttg atgagtgagt cgaagaagcc tgtcttgtat      900

gtgggaggtg ggtgtttgaa ttctagtgag gagttgagga gatttgttga gttgacaggg      960

attccggtgg ctagtacttt gatggggttg gggtcttacc cttgtaatga tgaactgtct     1020

cttcatatgt tggggatgca cgggactgtt tatgccaatt atgcggtgga taaggcggat     1080

ttgttgcttg ctttcggggt taggtttgat gatcgtgtga ccgggaagct cgaggcgttt     1140

gctagccgtg ctaagattgt gcatattgat attgactctg ctgagattgg gaagaacaag     1200

cagccccatg tgtccatttg tgctgatgtt aaattggcat tgcggggtat gaataagatt     1260

ctggagtcta gaatagggaa gctgaatttg gatttctcca agtggagaga agaattaggt     1320

gagcagaaga aggaattccc actgagtttt aagacatttg gggatgcaat tcctccacaa     1380

tatgccattc aggtgcttga tgagttgacc aatggtaatg ctattataag tactggtgtt     1440

gggcagcacc aaatgtgggc tgcgcagcat tacaagtaca gaaaccctcg ccaatggctg     1500

acctctggtg ggttgggggc tatggggttt gggctaccag ccgccattgg agctgcagtt     1560

gctcgaccag atgcagtggt tgtcgatatt gatggggatg gcagttttat tatgaatgtt     1620

caagagttgg ctacaattag ggtggaaaat ctcccagtta agataatgct gctaaacaat     1680

caacatttag gtatggttgt ccaattggaa gataggttct ataaagctaa ccgggcacat     1740

acataccttg gaaacccttc caaatctgct gatatcttcc ctgatatgct caaattcgct     1800

gaggcatgtg atattccttc tgcccgtgtt agcaacgtgg ctgatttgag ggccgccatt     1860

caaacaatgt tggatactcc agggccgtac ctgctcgatg tgattgtacc gcatcaagag     1920

catgtgttgc ctatgattcc aagtggtgcc ggtttcaagg ataccattac agagggtgat     1980

ggaagaacct cttattga                                                   1998


<210>  12
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein of mutated als gene (P188S and W569L)

<400>  12

Met Ala Ala Thr Phe Thr Asn Pro Thr Phe Ser Pro Ser Ser Thr Pro 
1               5                   10                  15      


Leu Thr Lys Thr Leu Lys Ser Gln Ser Ser Ile Ser Ser Thr Leu Pro 
            20                  25                  30          


Phe Ser Thr Pro Pro Lys Thr Pro Thr Pro Leu Phe His Arg Pro Leu 
        35                  40                  45              


Gln Ile Ser Ser Ser Gln Ser His Lys Ser Ser Ala Ile Lys Thr Gln 
    50                  55                  60                  


Thr Gln Ala Pro Ser Ser Pro Ala Ile Glu Asp Ser Ser Phe Val Ser 
65                  70                  75                  80  


Arg Phe Gly Pro Asp Glu Pro Arg Lys Gly Ser Asp Val Leu Val Glu 
                85                  90                  95      


Ala Leu Glu Arg Glu Gly Val Thr Asn Val Phe Ala Tyr Pro Gly Gly 
            100                 105                 110         


Ala Ser Met Glu Ile His Gln Ala Leu Thr Arg Ser Lys Thr Ile Arg 
        115                 120                 125             


Asn Val Leu Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly 
    130                 135                 140                 


Tyr Ala Arg Ala Thr Gly Lys Val Gly Val Cys Ile Ala Thr Ser Gly 
145                 150                 155                 160 


Pro Gly Ala Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp 
                165                 170                 175     


Ser Val Pro Leu Val Ala Ile Thr Gly Gln Val Ser Arg Arg Met Ile 
            180                 185                 190         


Gly Thr Asp Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser 
        195                 200                 205             


Ile Thr Lys His Asn Tyr Leu Val Leu Asp Val Glu Asp Ile Pro Arg 
    210                 215                 220                 


Ile Val Lys Glu Ala Phe Phe Leu Ala Asn Ser Gly Arg Pro Gly Pro 
225                 230                 235                 240 


Val Leu Ile Asp Leu Pro Lys Asp Ile Gln Gln Gln Leu Val Val Pro 
                245                 250                 255     


Asp Trp Asp Arg Pro Phe Lys Leu Gly Gly Tyr Met Ser Arg Leu Pro 
            260                 265                 270         


Lys Ser Lys Phe Ser Thr Asn Glu Val Gly Leu Leu Glu Gln Ile Val 
        275                 280                 285             


Arg Leu Met Ser Glu Ser Lys Lys Pro Val Leu Tyr Val Gly Gly Gly 
    290                 295                 300                 


Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly 
305                 310                 315                 320 


Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser Tyr Pro Cys Asn 
                325                 330                 335     


Asp Glu Leu Ser Leu His Met Leu Gly Met His Gly Thr Val Tyr Ala 
            340                 345                 350         


Asn Tyr Ala Val Asp Lys Ala Asp Leu Leu Leu Ala Phe Gly Val Arg 
        355                 360                 365             


Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala Phe Ala Ser Arg Ala 
    370                 375                 380                 


Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu Ile Gly Lys Asn Lys 
385                 390                 395                 400 


Gln Pro His Val Ser Ile Cys Ala Asp Val Lys Leu Ala Leu Arg Gly 
                405                 410                 415     


Met Asn Lys Ile Leu Glu Ser Arg Ile Gly Lys Leu Asn Leu Asp Phe 
            420                 425                 430         


Ser Lys Trp Arg Glu Glu Leu Gly Glu Gln Lys Lys Glu Phe Pro Leu 
        435                 440                 445             


Ser Phe Lys Thr Phe Gly Asp Ala Ile Pro Pro Gln Tyr Ala Ile Gln 
    450                 455                 460                 


Val Leu Asp Glu Leu Thr Asn Gly Asn Ala Ile Ile Ser Thr Gly Val 
465                 470                 475                 480 


Gly Gln His Gln Met Trp Ala Ala Gln His Tyr Lys Tyr Arg Asn Pro 
                485                 490                 495     


Arg Gln Trp Leu Thr Ser Gly Gly Leu Gly Ala Met Gly Phe Gly Leu 
            500                 505                 510         


Pro Ala Ala Ile Gly Ala Ala Val Ala Arg Pro Asp Ala Val Val Val 
        515                 520                 525             


Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn Val Gln Glu Leu Ala 
    530                 535                 540                 


Thr Ile Arg Val Glu Asn Leu Pro Val Lys Ile Met Leu Leu Asn Asn 
545                 550                 555                 560 


Gln His Leu Gly Met Val Val Gln Leu Glu Asp Arg Phe Tyr Lys Ala 
                565                 570                 575     


Asn Arg Ala His Thr Tyr Leu Gly Asn Pro Ser Lys Ser Ala Asp Ile 
            580                 585                 590         


Phe Pro Asp Met Leu Lys Phe Ala Glu Ala Cys Asp Ile Pro Ser Ala 
        595                 600                 605             


Arg Val Ser Asn Val Ala Asp Leu Arg Ala Ala Ile Gln Thr Met Leu 
    610                 615                 620                 


Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile Val Pro His Gln Glu 
625                 630                 635                 640 


His Val Leu Pro Met Ile Pro Ser Gly Ala Gly Phe Lys Asp Thr Ile 
                645                 650                 655     


Thr Glu Gly Asp Gly Arg Thr Ser Tyr 
            660                 665 


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BvEPSPS_Ex_2_for

<400>  13
ggaaatttcc atcctaacga g                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BvEPSPS_Ex_2_rev

<400>  14
gcaagaggaa acaagtctcc a                                                 21


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BvEPSPS_Ex2_Seqf

<400>  15
catcctaacg agaattatgc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BvEPSPS_Ex2_Seqr

<400>  16
gtctccacac aaaataaaag                                                   20


<210>  17
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KASP-marker s1txepss02 - Primer_Allel_C

<400>  17
gaaggtgacc aagttcatgc tcagcaactg cagctgtcaa tgg                         43


<210>  18
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KASP-marker s1txepss02 - Primer_Allel_T

<400>  18
gaaggtcgga gtcaacggat taacagcaac tgcagctgtc aatga                       45


<210>  19
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KASP-marker s1txepss02 - Primer_Common

<400>  19
ctttttcttg gaaatgcagg aacagcaat                                         29


<210>  20
<211>  517
<212>  PRT
<213>  Beta vulgaris

<400>  20

Met Ala Gln Ala Ser Thr Ile Asn Asn Gly Val Lys Ser Thr Gln Leu 
1               5                   10                  15      


Cys Pro Asn Leu Pro Lys Thr His Leu Ser Lys Ser Ser Lys Ser Val 
            20                  25                  30          


Lys Phe Gly Ser Asn Leu Arg Phe Ser Pro Lys Leu Lys Ser Phe Asn 
        35                  40                  45              


Asn Glu Arg Val Gly Gly Asn Ser Ser Val Val Phe Arg Val Arg Ala 
    50                  55                  60                  


Ser Val Ala Ala Ala Ala Glu Lys Ser Ser Thr Val Pro Glu Ile Val 
65                  70                  75                  80  


Leu Gln Pro Ile Lys Glu Ile Ser Gly Thr Ile Gln Leu Pro Gly Ser 
                85                  90                  95      


Lys Ser Leu Ser Asn Arg Ile Leu Leu Leu Ala Ala Leu Ser Gln Gly 
            100                 105                 110         


Thr Thr Val Val Asp Asn Leu Leu Tyr Ser Asp Asp Ile Arg Tyr Met 
        115                 120                 125             


Leu Asp Ala Leu Arg Thr Leu Gly Leu Asn Val Glu Asp Asp Asn Ile 
    130                 135                 140                 


Ala Lys Arg Ala Ile Val Glu Gly Cys Gly Gly Leu Phe Pro Val Gly 
145                 150                 155                 160 


Lys Asp Gly Lys Glu Ile Glu Leu Phe Leu Gly Asn Ala Gly Thr Ala 
                165                 170                 175     


Met Arg Pro Leu Thr Ala Ala Val Ala Val Ala Gly Gly Asn Ser Ser 
            180                 185                 190         


Tyr Val Leu Asp Gly Val Pro Arg Met Arg Glu Arg Pro Ile Gly Asp 
        195                 200                 205             


Leu Val Ala Gly Leu Lys Gln Leu Gly Ala Asp Val Asp Cys Tyr Leu 
    210                 215                 220                 


Gly Thr Asn Cys Pro Pro Val Arg Val Asn Ala Lys Gly Gly Leu Pro 
225                 230                 235                 240 


Gly Gly Lys Val Lys Leu Ser Gly Ser Val Ser Ser Gln Tyr Leu Thr 
                245                 250                 255     


Ala Leu Leu Met Ala Thr Pro Leu Gly Leu Gly Asp Val Glu Val Glu 
            260                 265                 270         


Ile Ile Asp Lys Leu Ile Ser Val Pro Tyr Val Glu Met Thr Ile Lys 
        275                 280                 285             


Leu Met Glu Arg Phe Gly Val Ser Val Glu His Ser Ala Asp Trp Gly 
    290                 295                 300                 


Arg Phe Leu Ile Arg Gly Gly Gln Lys Tyr Lys Ser Pro Gly Asn Ala 
305                 310                 315                 320 


Tyr Val Glu Gly Asp Ala Ser Ser Ala Ser Tyr Phe Ile Gly Gly Ala 
                325                 330                 335     


Ala Val Thr Gly Gly Thr Val Thr Val Glu Gly Cys Gly Thr Ser Ser 
            340                 345                 350         


Leu Gln Gly Asp Val Lys Phe Ala Glu Val Leu Glu Lys Met Gly Cys 
        355                 360                 365             


Lys Val Ser Trp Thr Glu Asn Ser Val Thr Val Thr Gly Pro Pro Arg 
    370                 375                 380                 


Asp Ala Ser Gly Arg Lys His Leu Arg Ala Val Asp Val Asn Met Asn 
385                 390                 395                 400 


Lys Met Pro Asp Val Ala Met Thr Leu Ala Val Val Ala Leu Tyr Ala 
                405                 410                 415     


Asp Gly Pro Thr Thr Ile Arg Asp Val Ala Ser Trp Arg Val Lys Glu 
            420                 425                 430         


Thr Glu Arg Met Ile Ala Ile Cys Thr Glu Leu Arg Lys Leu Gly Ala 
        435                 440                 445             


Thr Val Glu Glu Gly Ser Asp Tyr Cys Val Ile Thr Pro Pro Glu Lys 
    450                 455                 460                 


Leu Asn Val Thr Ala Ile Asp Thr Tyr Asp Asp His Arg Met Ala Met 
465                 470                 475                 480 


Ala Phe Ser Leu Ala Ala Cys Ala Asp Val Pro Val Thr Ile Lys Asp 
                485                 490                 495     


Pro Gly Cys Thr Arg Lys Thr Phe Pro Asp Tyr Phe Asp Val Leu Glu 
            500                 505                 510         


Arg Phe Ala Lys His 
        515         


<210>  21
<211>  665
<212>  PRT
<213>  Beta vulgaris

<400>  21

Met Ala Ala Thr Phe Thr Asn Pro Thr Phe Ser Pro Ser Ser Thr Pro 
1               5                   10                  15      


Leu Thr Lys Thr Leu Lys Ser Gln Ser Ser Ile Ser Ser Thr Leu Pro 
            20                  25                  30          


Phe Ser Thr Pro Pro Lys Thr Pro Thr Pro Leu Phe His Arg Pro Leu 
        35                  40                  45              


Gln Ile Ser Ser Ser Gln Ser His Lys Ser Ser Ala Ile Lys Thr Gln 
    50                  55                  60                  


Thr Gln Ala Pro Ser Ser Pro Ala Ile Glu Asp Ser Ser Phe Val Ser 
65                  70                  75                  80  


Arg Phe Gly Pro Asp Glu Pro Arg Lys Gly Ser Asp Val Leu Val Glu 
                85                  90                  95      


Ala Leu Glu Arg Glu Gly Val Thr Asn Val Phe Ala Tyr Pro Gly Gly 
            100                 105                 110         


Ala Ser Met Glu Ile His Gln Ala Leu Thr Arg Ser Lys Thr Ile Arg 
        115                 120                 125             


Asn Val Leu Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly 
    130                 135                 140                 


Tyr Ala Arg Ala Thr Gly Lys Val Gly Val Cys Ile Ala Thr Ser Gly 
145                 150                 155                 160 


Pro Gly Ala Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp 
                165                 170                 175     


Ser Val Pro Leu Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile 
            180                 185                 190         


Gly Thr Asp Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser 
        195                 200                 205             


Ile Thr Lys His Asn Tyr Leu Val Leu Asp Val Glu Asp Ile Pro Arg 
    210                 215                 220                 


Ile Val Lys Glu Ala Phe Phe Leu Ala Asn Ser Gly Arg Pro Gly Pro 
225                 230                 235                 240 


Val Leu Ile Asp Leu Pro Lys Asp Ile Gln Gln Gln Leu Val Val Pro 
                245                 250                 255     


Asp Trp Asp Arg Pro Phe Lys Leu Gly Gly Tyr Met Ser Arg Leu Pro 
            260                 265                 270         


Lys Ser Lys Phe Ser Thr Asn Glu Val Gly Leu Leu Glu Gln Ile Val 
        275                 280                 285             


Arg Leu Met Ser Glu Ser Lys Lys Pro Val Leu Tyr Val Gly Gly Gly 
    290                 295                 300                 


Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly 
305                 310                 315                 320 


Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser Tyr Pro Cys Asn 
                325                 330                 335     


Asp Glu Leu Ser Leu His Met Leu Gly Met His Gly Thr Val Tyr Ala 
            340                 345                 350         


Asn Tyr Ala Val Asp Lys Ala Asp Leu Leu Leu Ala Phe Gly Val Arg 
        355                 360                 365             


Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala Phe Ala Ser Arg Ala 
    370                 375                 380                 


Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu Ile Gly Lys Asn Lys 
385                 390                 395                 400 


Gln Pro His Val Ser Ile Cys Ala Asp Val Lys Leu Ala Leu Arg Gly 
                405                 410                 415     


Met Asn Lys Ile Leu Glu Ser Arg Ile Gly Lys Leu Asn Leu Asp Phe 
            420                 425                 430         


Ser Lys Trp Arg Glu Glu Leu Gly Glu Gln Lys Lys Glu Phe Pro Leu 
        435                 440                 445             


Ser Phe Lys Thr Phe Gly Asp Ala Ile Pro Pro Gln Tyr Ala Ile Gln 
    450                 455                 460                 


Val Leu Asp Glu Leu Thr Asn Gly Asn Ala Ile Ile Ser Thr Gly Val 
465                 470                 475                 480 


Gly Gln His Gln Met Trp Ala Ala Gln His Tyr Lys Tyr Arg Asn Pro 
                485                 490                 495     


Arg Gln Trp Leu Thr Ser Gly Gly Leu Gly Ala Met Gly Phe Gly Leu 
            500                 505                 510         


Pro Ala Ala Ile Gly Ala Ala Val Ala Arg Pro Asp Ala Val Val Val 
        515                 520                 525             


Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn Val Gln Glu Leu Ala 
    530                 535                 540                 


Thr Ile Arg Val Glu Asn Leu Pro Val Lys Ile Met Leu Leu Asn Asn 
545                 550                 555                 560 


Gln His Leu Gly Met Val Val Gln Trp Glu Asp Arg Phe Tyr Lys Ala 
                565                 570                 575     


Asn Arg Ala His Thr Tyr Leu Gly Asn Pro Ser Lys Ser Ala Asp Ile 
            580                 585                 590         


Phe Pro Asp Met Leu Lys Phe Ala Glu Ala Cys Asp Ile Pro Ser Ala 
        595                 600                 605             


Arg Val Ser Asn Val Ala Asp Leu Arg Ala Ala Ile Gln Thr Met Leu 
    610                 615                 620                 


Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile Val Pro His Gln Glu 
625                 630                 635                 640 


His Val Leu Pro Met Ile Pro Ser Gly Ala Gly Phe Lys Asp Thr Ile 
                645                 650                 655     


Thr Glu Gly Asp Gly Arg Thr Ser Tyr 
            660                 665 


