                         SEQUENCE LISTING

<110>  PIERRE FABRE MEDICAMENT
 
<120>  NEW IMMUNOCYTOKINES FOR THE TREATMENT OF CANCER

<130>  B376706PCTD38826

<150>  EP 19305336.0
<151>  2019-03-19

<150>  US 62/738391
<151>  2018-09-28

<160>  186   

<170>  PatentIn version 3.5

<210>  1
<211>  354
<212>  DNA
<213>  Artificial

<220>
<223>  Atezolizumab Heavy Chain Variable Region

<400>  1
gaggtccagc ttgtcgagtc aggtggaggt cttgtacagc caggaggaag tttgcgtttg       60

agctgcgctg cttctgggtt cacttttagc gactcctgga tccactgggt cagacaggca      120

cctggaaagg ggctggaatg ggttgcatgg atttccccct acggcggctc tacttattac      180

gctgacagtg ttaaaggcag gtttaccata agcgccgaca cctccaaaaa caccgcctac      240

ctgcagatga atagtctgcg agccgaagat actgccgtgt actattgcgc ccggcgccat      300

tggcccggcg gctttgatta ttgggggcaa gggacactgg tgacagtgtc tgct            354


<210>  2
<211>  118
<212>  PRT
<213>  Artificial

<220>
<223>  Atezolizumab Heavy Chain Variable Region

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  3
<211>  320
<212>  DNA
<213>  Artificial

<220>
<223>  Atezolizumab Light Chain Variable Region

<400>  3
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa                                                  320


<210>  4
<211>  107
<212>  PRT
<213>  Artificial

<220>
<223>  Atezolizumab Light Chain Variable Region

<400>  4

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  5
<211>  357
<212>  DNA
<213>  Artificial

<220>
<223>  Ganitumab Heavy Chain Variable Region

<400>  5
caggtgcagc tgcaggagtc gggcccagga ctggtgaagc cttcggggac cctgtccctc       60

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      120

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      300

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagc         357


<210>  6
<211>  119
<212>  PRT
<213>  Artificial

<220>
<223>  Ganitumab Heavy Chain Variable Region

<400>  6

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gly 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser Ile Ser Ser Ser 
            20                  25                  30          


Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser 
        115                 


<210>  7
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  Ganitumab Light Chain Variable Region

<400>  7
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc       60

atctcctgca ggtctagtca gagcctcctg catagtaatg gatacaacta tttggattgg      120

tacctgcaga agccagggca gtctccacag ctcctgatct atttgggttc taatcgggcc      180

tccggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgca tgcaaggtac acactggcct      300

ctgacgttcg gccaagggac caaggtggag atcaaa                                336


<210>  8
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  Ganitumab Light Chain Variable Region

<400>  8

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Gly 
                85                  90                  95      


Thr His Trp Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  9
<211>  351
<212>  DNA
<213>  Artificial

<220>
<223>  NHS76 Heavy Chain Variable Region

<400>  9
caggtgcagc tgcaggagtc cggcccagga ctggtgaagc cttcggagac cctgtccctc       60

acctgcgctg tctctggtta ctccatcagc agtggttact actggggctg gattcggcag      120

cccccaggga aggggctgga gtggattggg agtatctatc atagtgggag cacctactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca cgtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcagac acggccgtgt attactgtgc aagagggaag      300

tggtcgaagt ttgactattg gggccaaggc accctggtca ccgtctcttc a               351


<210>  10
<211>  117
<212>  PRT
<213>  Artificial

<220>
<223>  NHS76 Heavy Chain Variable Region

<400>  10

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Tyr Ser Ile Ser Ser Gly 
            20                  25                  30          


Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ser Ile Tyr His Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Trp Ser Lys Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  11
<211>  324
<212>  DNA
<213>  Artificial

<220>
<223>  NHS76 Light Chain Variable Region

<400>  11
tcctctgagc tgactcagga ccctgctgtg tctgtggcct tgggacagac agtcaggatc       60

acatgccaag gagacagcct cagaagctat tatgcaagct ggtaccagca gaagccagga      120

caggcccctg tacttgtcat ctatggtaaa aacaaccggc cctcagggat tccagaccga      180

ttctctggct ccagctcagg aaacacagct tccttgacca tcactggggc tcaggcggaa      240

gatgaggctg actattactg taactcccgg gacagcagtg gtaaccatgt ggtattcggc      300

ggagggacca agctgaccgt ccta                                             324


<210>  12
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  NHS76 Light Chain Variable Region

<400>  12

Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala 
            20                  25                  30          


Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Gly Lys Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105             


<210>  13
<211>  339
<212>  DNA
<213>  Artificial

<220>
<223>  9G4 Heavy Chain Variable Region

<400>  13
gaagtgatgt tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtacag cctctggatt cactttcagt acctatgaca tgtcttgggt tcgccagact      120

ccggagaaga ggctggagtg ggtctcaacc attagtagta gtcgtactta cacctactat      180

tcagccagtg tgaaggggcg attcaccatc tccagagaca atgccaggaa catcctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaattt      300

tctcactggg gccaagggac tctggtcact gtctctgca                             339


<210>  14
<211>  113
<212>  PRT
<213>  Artificial

<220>
<223>  9G4 Heavy Chain Variable Region

<400>  14

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ala 
    


<210>  15
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  9G4 Light Chain Variable Region

<400>  15
gatgttgtga tgacccagac tccactcact ttgtcggtta ccattggaca accagcctcc       60

atctcttgca agtcaagtca gagcctctta gatagtgatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaacg cgcctaatct atctggtgtc taaactggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaagatc      240

agcagagtgg aggctgagga tttgggagtt tattattgct ggcaaggtac acattttcct      300

cacacgttcg gaggggggac caagctggaa ataaaa                                336


<210>  16
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  9G4 Light Chain Variable Region

<400>  16

Asp Val Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Thr Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  17
<211>  987
<212>  DNA
<213>  Human

<400>  17
gcgtccacaa agggcccaag cgtgttcccg ctagccccca gcagcaagag caccagcggc       60

ggcacagccg ccctgggctg cctggtgaag gactacttcc ccgagcccgt gaccgtgtcc      120

tggaacagcg gagccctgac ctccggcgtg cacaccttcc ccgccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtggtgacc gtgcccagca gcagcctggg cacccagacc      240

tacatctgta acgtgaacca caagcccagc aacaccaagg tggacaagag agtggagccc      300

aagagctgtg acaagaccca cacctgtccc ccctgcccag cccccgagct gctgggcgga      360

cccagcgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag cagaaccccc      420

gaggtgacct gtgtggtggt ggacgtgtcc cacgaggacc cagaggtgaa gttcaactgg      480

tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga gcagtacaac      540

agcacctaca gggtggtgtc cgtgctgacc gtgctgcacc aggactggct gaacggcaag      600

gagtacaagt gtaaggtgtc caacaaggcc ctgccagccc caatcgaaaa gaccatcagc      660

aaggccaagg gccagccaag agagccccag gtgtacaccc tgccacccag cagggaggag      720

atgaccaaga accaggtgtc cctgacctgt ctggtgaagg gcttctaccc aagcgacatc      780

gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac ccccccagtg      840

ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa gagcagatgg      900

cagcagggca acgtgttcag ctgctccgtg atgcacgagg ccctgcacaa ccactacacc      960

cagaagagcc tgagcctgtc cccaggc                                          987


<210>  18
<211>  329
<212>  PRT
<213>  Human

<400>  18

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  19
<211>  978
<212>  DNA
<213>  Human

<400>  19
gcgtccacaa agggccccag cgtgttccct ctggccccct gcagcagaag caccagcgag       60

tctacagccg ccctgggatg cctggtcaag gactacttcc ccgagcccgt gaccgtgtcc      120

tggaactctg gcgccctgac cagcggcgtg cacacctttc cagccgtgct gcagagcagc      180

ggcctgtaca gcctgagcag cgtcgtgacc gtgcctagca gcagcctggg caccaagacc      240

tacacctgta acgtggacca caagcccagc aacaccaagg tggacaagcg ggtggaatct      300

aagtacggcc ctccctgccc cccctgccca gcccctgaat ttctgggcgg accctccgtg      360

ttcctgttcc ccccaaagcc caaggacacc ctgatgatca gccggacccc cgaagtgacc      420

tgcgtggtgg tggacgtgtc ccaggaagat cccgaggtcc agttcaactg gtacgtggac      480

ggcgtggaag tgcacaacgc caagaccaag cccagagagg aacagttcaa cagcacctac      540

cgggtggtgt ccgtgctgac cgtgctgcac caggactggc tgaacggcaa agagtacaag      600

tgcaaggtct ccaacaaggg cctgcccagc tccatcgaga aaaccatcag caaggccaag      660

ggccagcccc gcgagcccca ggtgtacaca ctgcccccca gccaggaaga gatgaccaag      720

aaccaggtgt ccctgacctg cctcgtgaag ggcttctacc ccagcgatat cgccgtggaa      780

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccctgt gctggacagc      840

gacggcagct tcttcctgta ctcccggctg accgtcgaca agtctagatg gcaggaaggc      900

aacgtcttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagtcc      960

ctgagcctga gcctggga                                                    978


<210>  20
<211>  326
<212>  PRT
<213>  Human

<400>  20

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly 
                325     


<210>  21
<211>  321
<212>  DNA
<213>  Human

<400>  21
cgtacggtgg ccgctcccag cgtgttcatc ttcccaccca gcgacgagca gctgaagtct       60

ggcaccgcca gcgtcgtgtg cctgctgaac aacttctacc cccgcgaggc caaggtgcag      120

tggaaggtgg acaacgccct gcagtccggc aacagccagg aaagcgtcac cgagcaggac      180

agcaaggact ccacctacag cctgagcagc accctgaccc tgagcaaggc cgactacgag      240

aagcacaagg tgtacgcctg cgaagtgacc caccagggcc tgtccagccc cgtgaccaag      300

agcttcaacc ggggcgagtg c                                                321


<210>  22
<211>  107
<212>  PRT
<213>  Human

<400>  22

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  23
<211>  318
<212>  DNA
<213>  Human

<400>  23
ggtcaaccca aggccgctcc cagcgtgacc ctgttccccc cctcgagcga ggagctgcag       60

gccaacaagg ccaccctggt gtgtctgatc agcgacttct acccaggcgc cgtgaccgtg      120

gcctggaagg ccgacagcag ccccgtgaag gccggcgtgg agaccaccac ccccagcaag      180

cagagcaaca acaagtacgc cgccagcagc tacctgagcc tgaccccaga gcagtggaag      240

agccacagga gctacagctg ccaggtcacc cacgagggca gcaccgtgga aaagaccgtg      300

gccccaaccg agtgctcc                                                    318


<210>  24
<211>  106
<212>  PRT
<213>  Human

<400>  24

Gly Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      


Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          


Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro 
        35                  40                  45              


Val Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  


Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys 
65                  70                  75                  80  


Ser His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      


Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105     


<210>  25
<211>  342
<212>  DNA
<213>  Human

<400>  25
aactgggtga atgtaataag tgatttgaaa aaaattgaag atcttattca atctatgcat       60

attgatgcta ctttatatac ggaaagtgat gttcacccca gttgcaaagt aacagcaatg      120

aagtgctttc tcttggagtt acaagttatt tcacttgagt ccggagatgc aagtattcat      180

gatacagtag aaaatctgat catcctagca aacaacagtt tgtcttctaa tgggaatgta      240

acagaatctg gatgcaaaga atgtgaggaa ctggaggaaa aaaatattaa agaatttttg      300

cagagttttg tacatattgt ccaaatgttc atcaacactt ct                         342


<210>  26
<211>  114
<212>  PRT
<213>  Human

<400>  26

Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
1               5                   10                  15      


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
            20                  25                  30          


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
        35                  40                  45              


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
    50                  55                  60                  


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
65                  70                  75                  80  


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
                85                  90                  95      


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
            100                 105                 110         


Thr Ser 
        


<210>  27
<211>  399
<212>  DNA
<213>  Human

<400>  27
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgact                             399


<210>  28
<211>  133
<212>  PRT
<213>  Human

<400>  28

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  29
<211>  207
<212>  DNA
<213>  Human

<400>  29
gcaccaatgg gctcagaccc tcccaccgcc tgctgctttt cttacaccgc gaggaaactt       60

cctcgcaact ttgtggtaga ttactatgag accagcagcc tctgctccca gccagctgtg      120

gtattccaaa ccaaaagaag caagcaagtc tgtgctgatc ccagtgaatc ctgggtccag      180

gagtacgtgt atgacctgga actgaac                                          207


<210>  30
<211>  69
<212>  PRT
<213>  Human

<400>  30

Ala Pro Met Gly Ser Asp Pro Pro Thr Ala Cys Cys Phe Ser Tyr Thr 
1               5                   10                  15      


Ala Arg Lys Leu Pro Arg Asn Phe Val Val Asp Tyr Tyr Glu Thr Ser 
            20                  25                  30          


Ser Leu Cys Ser Gln Pro Ala Val Val Phe Gln Thr Lys Arg Ser Lys 
        35                  40                  45              


Gln Val Cys Ala Asp Pro Ser Glu Ser Trp Val Gln Glu Tyr Val Tyr 
    50                  55                  60                  


Asp Leu Glu Leu Asn 
65                  


<210>  31
<211>  495
<212>  DNA
<213>  Human

<400>  31
tgtgatctgc ctcaaaccca cagcctgggt agcaggagga ccttgatgct cctggcacag       60

atgaggaaaa tctctctttt ctcctgcttg aaggacagac atgactttgg atttccccag      120

gaggagtttg gcaaccagtt ccaaaaggct gaaaccatcc ctgtcctcca tgagatgatc      180

cagcagatct tcaatctctt cagcacaaag gactcatctg ctgcttggga tgagaccctc      240

ctagacaaat tctacactga actctaccag cagctgaatg acctggaagc ctgtgtgata      300

cagggggtgg gggtgacaga gactcccctg atgaaggagg actccattct ggctgtgagg      360

aaatacttcc aaagaatcac tctctatctg aaagagaaga aatacagccc ttgtgcctgg      420

gaggttgtca gagcagaaat catgagatct ttttctttgt caacaaactt gcaagaaagt      480

ttaagaagta aggaa                                                       495


<210>  32
<211>  165
<212>  PRT
<213>  Human

<400>  32

Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met 
1               5                   10                  15      


Leu Leu Ala Gln Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln 
        35                  40                  45              


Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe 
    50                  55                  60                  


Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu 
65                  70                  75                  80  


Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu 
                85                  90                  95      


Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys 
            100                 105                 110         


Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu 
        115                 120                 125             


Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg 
    130                 135                 140                 


Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser 
145                 150                 155                 160 


Leu Arg Ser Lys Glu 
                165 


<210>  33
<211>  513
<212>  DNA
<213>  Oplophorus gracilirostris

<400>  33
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcg                                   513


<210>  34
<211>  173
<212>  PRT
<213>  Oplophorus gracilirostris

<400>  34

Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln 
1               5                   10                  15      


Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser 
            20                  25                  30          


Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile 
        35                  40                  45              


Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile 
    50                  55                  60                  


Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile 
65                  70                  75                  80  


Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu 
                85                  90                  95      


His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp 
            100                 105                 110         


Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys 
        115                 120                 125             


Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu 
    130                 135                 140                 


Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
145                 150                 155                 160 


Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170             


<210>  35
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  35
ccgctgggcc tggcgggc                                                     18


<210>  36
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  36

Pro Leu Gly Leu Ala Gly 
1               5       


<210>  37
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  37
ggcccgctgg gcattgcggg ccag                                              24


<210>  38
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  38

Gly Pro Leu Gly Ile Ala Gly Gln 
1               5               


<210>  39
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  39
ggcccgctgg gcctgtgggc gcag                                              24


<210>  40
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  40

Gly Pro Leu Gly Leu Trp Ala Gln 
1               5               


<210>  41
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  41
ggcccgctgg gcatgctgag ccag                                              24


<210>  42
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  42

Gly Pro Leu Gly Met Leu Ser Gln 
1               5               


<210>  43
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  43
ccggtgggcc tgattggc                                                     18


<210>  44
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  44

Pro Val Gly Leu Ile Gly 
1               5       


<210>  45
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  45
ggcattgtgg gaccgctg                                                     18


<210>  46
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  46

Gly Ile Val Gly Pro Leu 
1               5       


<210>  47
<211>  6
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  47
ctcgag                                                                   6


<210>  48
<211>  2
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  48

Leu Glu 
1       


<210>  49
<211>  1704
<212>  DNA
<213>  Artificial

<220>
<223>  Heavy Chain of Ganitumab-PVGLIG-IL15

<400>  49
caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc       60

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      120

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      300

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      360

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      420

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      480

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      540

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      600

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      660

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      720

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      780

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      840

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      900

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag      960

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1020

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1080

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1140

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1200

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1260

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1320

aagagcctga gcctgtcccc aggcccggtg ggcctgattg gcaactgggt gaatgtaata     1380

agtgatttga aaaaaattga agatcttatt caatctatgc atattgatgc tactttatat     1440

acggaaagtg atgttcaccc cagttgcaaa gtaacagcaa tgaagtgctt tctcttggag     1500

ttacaagtta tttcacttga gtccggagat gcaagtattc atgatacagt agaaaatctg     1560

atcatcctag caaacaacag tttgtcttct aatgggaatg taacagaatc tggatgcaaa     1620

gaatgtgagg aactggagga aaaaaatatt aaagaatttt tgcagagttt tgtacatatt     1680

gtccaaatgt tcatcaacac ttct                                            1704


<210>  50
<211>  568
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy Chain of Ganitumab-PVGLIG-IL15

<400>  50

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gly 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser Ile Ser Ser Ser 
            20                  25                  30          


Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


Pro Val Gly Leu Ile Gly Asn Trp Val Asn Val Ile Ser Asp Leu Lys 
    450                 455                 460                 


Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr 
465                 470                 475                 480 


Thr Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys 
                485                 490                 495     


Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser 
            500                 505                 510         


Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu 
        515                 520                 525             


Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu 
    530                 535                 540                 


Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile 
545                 550                 555                 560 


Val Gln Met Phe Ile Asn Thr Ser 
                565             


<210>  51
<211>  1698
<212>  DNA
<213>  Artificial

<220>
<223>  Heavy Chain of NHS76-PVGLIG-IL15

<400>  51
caggtgcagc tgcaggagtc cggcccagga ctggtgaagc cttcggagac cctgtccctc       60

acctgcgctg tctctggtta ctccatcagc agtggttact actggggctg gattcggcag      120

cccccaggga aggggctgga gtggattggg agtatctatc atagtgggag cacctactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca cgtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcagac acggccgtgt attactgtgc aagagggaag      300

tggtcgaagt ttgactattg gggccaaggc accctggtca ccgtctcttc agcgtccaca      360

aagggcccaa gcgtgttccc gctagccccc agcagcaaga gcaccagcgg cggcacagcc      420

gccctgggct gcctggtgaa ggactacttc cccgagcccg tgaccgtgtc ctggaacagc      480

ggagccctga cctccggcgt gcacaccttc cccgccgtgc tgcagagcag cggcctgtac      540

agcctgagca gcgtggtgac cgtgcccagc agcagcctgg gcacccagac ctacatctgt      600

aacgtgaacc acaagcccag caacaccaag gtggacaaga gagtggagcc caagagctgt      660

gacaagaccc acacctgtcc cccctgccca gcccccgagc tgctgggcgg acccagcgtg      720

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      780

tgtgtggtgg tggacgtgtc ccacgaggac ccagaggtga agttcaactg gtacgtggac      840

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagtacaa cagcacctac      900

agggtggtgt ccgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      960

tgtaaggtgt ccaacaaggc cctgccagcc ccaatcgaaa agaccatcag caaggccaag     1020

ggccagccaa gagagcccca ggtgtacacc ctgccaccca gcagggagga gatgaccaag     1080

aaccaggtgt ccctgacctg tctggtgaag ggcttctacc caagcgacat cgccgtggag     1140

tgggagagca acggccagcc cgagaacaac tacaagacca cccccccagt gctggacagc     1200

gacggcagct tcttcctgta cagcaagctg accgtggaca agagcagatg gcagcagggc     1260

aacgtgttca gctgctccgt gatgcacgag gccctgcaca accactacac ccagaagagc     1320

ctgagcctgt ccccaggccc ggtgggcctg attggcaact gggtgaatgt aataagtgat     1380

ttgaaaaaaa ttgaagatct tattcaatct atgcatattg atgctacttt atatacggaa     1440

agtgatgttc accccagttg caaagtaaca gcaatgaagt gctttctctt ggagttacaa     1500

gttatttcac ttgagtccgg agatgcaagt attcatgata cagtagaaaa tctgatcatc     1560

ctagcaaaca acagtttgtc ttctaatggg aatgtaacag aatctggatg caaagaatgt     1620

gaggaactgg aggaaaaaaa tattaaagaa tttttgcaga gttttgtaca tattgtccaa     1680

atgttcatca acacttct                                                   1698


<210>  52
<211>  566
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy Chain of NHS76-PVGLIG-IL15

<400>  52

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Tyr Ser Ile Ser Ser Gly 
            20                  25                  30          


Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ser Ile Tyr His Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Trp Ser Lys Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val 
        435                 440                 445             


Gly Leu Ile Gly Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile 
    450                 455                 460                 


Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu 
465                 470                 475                 480 


Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu 
                485                 490                 495     


Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His 
            500                 505                 510         


Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser 
        515                 520                 525             


Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu 
    530                 535                 540                 


Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln 
545                 550                 555                 560 


Met Phe Ile Asn Thr Ser 
                565     


<210>  53
<211>  657
<212>  DNA
<213>  Artificial

<220>
<223>  Ganitumab Light Chain

<400>  53
gatgttgtga tgactcagtc tccactctcc ctgcccgtca cccctggaga gccggcctcc       60

atctcctgca ggtctagtca gagcctcctg catagtaatg gatacaacta tttggattgg      120

tacctgcaga agccagggca gtctccacag ctcctgatct atttgggttc taatcgggcc      180

tccggggtcc ctgacaggtt cagtggcagt ggatcaggca cagattttac actgaaaatc      240

agcagggtgg aggctgagga tgttggggtt tattactgca tgcaaggtac acactggcct      300

ctgacgttcg gccaagggac caaggtggag atcaaacgta cggtggccgc tcccagcgtg      360

ttcatcttcc cacccagcga cgagcagctg aagtctggca ccgccagcgt cgtgtgcctg      420

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      480

tccggcaaca gccaggaaag cgtcaccgag caggacagca aggactccac ctacagcctg      540

agcagcaccc tgaccctgag caaggccgac tacgagaagc acaaggtgta cgcctgcgaa      600

gtgacccacc agggcctgtc cagccccgtg accaagagct tcaaccgggg cgagtgc         657


<210>  54
<211>  219
<212>  PRT
<213>  Artificial

<220>
<223>  Ganitumab Light Chain

<400>  54

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Tyr Asn Tyr Leu Asp Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Leu Gly Ser Asn Arg Ala Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Gly 
                85                  90                  95      


Thr His Trp Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  55
<211>  645
<212>  DNA
<213>  Artificial

<220>
<223>  NHS76 Light Chain

<400>  55
tcctctgagc tgactcagga ccctgctgtg tctgtggcct tgggacagac agtcaggatc       60

acatgccaag gagacagcct cagaagctat tatgcaagct ggtaccagca gaagccagga      120

caggcccctg tacttgtcat ctatggtaaa aacaaccggc cctcagggat tccagaccga      180

ttctctggct ccagctcagg aaacacagct tccttgacca tcactggggc tcaggcggaa      240

gatgaggctg actattactg taactcccgg gacagcagtg gtaaccatgt ggtattcggc      300

ggagggacca agctgaccgt cctaggtcaa cccaaggccg ctcccagcgt gaccctgttc      360

cccccctcga gcgaggagct gcaggccaac aaggccaccc tggtgtgtct gatcagcgac      420

ttctacccag gcgccgtgac cgtggcctgg aaggccgaca gcagccccgt gaaggccggc      480

gtggagacca ccacccccag caagcagagc aacaacaagt acgccgccag cagctacctg      540

agcctgaccc cagagcagtg gaagagccac aggagctaca gctgccaggt cacccacgag      600

ggcagcaccg tggaaaagac cgtggcccca accgagtgct cctga                      645


<210>  56
<211>  214
<212>  PRT
<213>  Artificial

<220>
<223>  NHS76 Light Chain

<400>  56

Ser Ser Glu Leu Thr Gln Asp Pro Ala Val Ser Val Ala Leu Gly Gln 
1               5                   10                  15      


Thr Val Arg Ile Thr Cys Gln Gly Asp Ser Leu Arg Ser Tyr Tyr Ala 
            20                  25                  30          


Ser Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Val Leu Val Ile Tyr 
        35                  40                  45              


Gly Lys Asn Asn Arg Pro Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser 
    50                  55                  60                  


Ser Ser Gly Asn Thr Ala Ser Leu Thr Ile Thr Gly Ala Gln Ala Glu 
65                  70                  75                  80  


Asp Glu Ala Asp Tyr Tyr Cys Asn Ser Arg Asp Ser Ser Gly Asn His 
                85                  90                  95      


Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys 
            100                 105                 110         


Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln 
        115                 120                 125             


Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly 
    130                 135                 140                 


Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly 
145                 150                 155                 160 


Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala 
                165                 170                 175     


Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg Ser 
            180                 185                 190         


Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val 
        195                 200                 205             


Ala Pro Thr Glu Cys Ser 
    210                 


<210>  57
<211>  1875
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-NanoLuc (C-terminal fusion)

<400>  57
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcatgctg agccagctcg agatggtctt cacactcgaa     1380

gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag     1440

ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt     1500

gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt     1560

ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat     1620

gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg     1680

aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag     1740

atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac     1800

cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc     1860

gaacgcattc tggcg                                                      1875


<210>  58
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-NanoLuc (C-terminal fusion)

<400>  58

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Met Leu Ser Gln Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly 
    450                 455                 460                 


Asp Trp Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln 
465                 470                 475                 480 


Gly Gly Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro 
                485                 490                 495     


Ile Gln Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile 
            500                 505                 510         


His Val Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln 
        515                 520                 525             


Ile Glu Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe 
    530                 535                 540                 


Lys Val Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro 
545                 550                 555                 560 


Asn Met Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe 
                565                 570                 575     


Asp Gly Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys 
            580                 585                 590         


Ile Ile Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg 
        595                 600                 605             


Val Thr Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu 
    610                 615                 620                 


Ala 
625 


<210>  59
<211>  1185
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-NanoLuc (C-terminal fusion)

<400>  59
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcatgctg      660

agccagctcg agatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc      720

ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc      780

ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc      840

gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa      900

aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat      960

ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat     1020

gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc     1080

aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc     1140

atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcg                     1185


<210>  60
<211>  395
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-NanoLuc (C-terminal fusion)

<400>  60

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Met Leu Ser Gln Leu Glu 
    210                 215                 220                 


Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
225                 230                 235                 240 


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
                245                 250                 255     


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
            260                 265                 270         


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
        275                 280                 285             


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
    290                 295                 300                 


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
305                 310                 315                 320 


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
                325                 330                 335     


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
            340                 345                 350         


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
        355                 360                 365             


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
    370                 375                 380                 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390                 395 


<210>  61
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-NanoLuc (N-terminal fusion)

<400>  61
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcatgct gagccaggaa      540

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      600

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      660

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      720

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      780

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      840

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      900

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      960

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct     1020

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac     1080

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1140

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1200

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1260

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1320

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1380

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1440

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1500

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1560

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1620

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1680

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1740

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1800

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1860

agcctggga                                                             1869


<210>  62
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-NanoLuc (N-terminal fusion)

<400>  62

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Met 
                165                 170                 175     


Leu Ser Gln Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        195                 200                 205             


Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 
        275                 280                 285             


Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser 
    290                 295                 300                 


Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala 
305                 310                 315                 320 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                325                 330                 335     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            340                 345                 350         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        355                 360                 365             


Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His 
    370                 375                 380                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly 
385                 390                 395                 400 


Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser 
                405                 410                 415     


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            420                 425                 430         


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro 
        435                 440                 445             


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    450                 455                 460                 


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val 
465                 470                 475                 480 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                485                 490                 495     


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr 
            500                 505                 510         


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        515                 520                 525             


Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    530                 535                 540                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
545                 550                 555                 560 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
                565                 570                 575     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser 
            580                 585                 590         


Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        595                 600                 605             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620             


<210>  63
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-NanoLuc (N-terminal fusion)

<400>  63
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcatgct gagccaggac      540

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      600

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      660

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      720

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      780

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      840

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      900

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      960

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa     1020

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg     1080

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1140

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1179


<210>  64
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-NanoLuc (N-terminal fusion)

<400>  64

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Met 
                165                 170                 175     


Leu Ser Gln Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            180                 185                 190         


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
        195                 200                 205             


Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
    210                 215                 220                 


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
225                 230                 235                 240 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                245                 250                 255     


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr 
            260                 265                 270         


His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
        275                 280                 285             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    290                 295                 300                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
305                 310                 315                 320 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                325                 330                 335     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            340                 345                 350         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        355                 360                 365             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    370                 375                 380                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
385                 390             


<210>  65
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-IL2 (C-terminal fusion)

<400>  65
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcatgctg agccaggcac ctacctcgag ttctacaaag     1380

aaaacacagc tacaactgga gcatttactg ctggatttac agatgatttt gaatggaatt     1440

aataattaca agaatcccaa actcaccagg atgctcacat ttaagtttta catgcccaag     1500

aaggccacag aactgaaaca tcttcagtgt ctagaagaag aactcaaacc tctggaggaa     1560

gtgctaaatt tagctcaaag caaaaacttt cacttaagac ccagggactt aatcagcaat     1620

atcaacgtaa tagttctgga actaaaggga tctgaaacaa cattcatgtg tgaatatgct     1680

gatgagacag caaccattgt agaatttctg aacagatgga ttaccttttg tcaaagcatc     1740

atctcaacac tgact                                                      1755


<210>  66
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-IL2 (C-terminal fusion)

<400>  66

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Met Leu Ser Gln Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
    450                 455                 460                 


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
465                 470                 475                 480 


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
                485                 490                 495     


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
            500                 505                 510         


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
        515                 520                 525             


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
    530                 535                 540                 


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
545                 550                 555                 560 


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
                565                 570                 575     


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585 


<210>  67
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-IL2 (C-terminal fusion)

<400>  67
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcatgctg      660

agccaggcac ctacctcgag ttctacaaag aaaacacagc tacaactgga gcatttactg      720

ctggatttac agatgatttt gaatggaatt aataattaca agaatcccaa actcaccagg      780

atgctcacat ttaagtttta catgcccaag aaggccacag aactgaaaca tcttcagtgt      840

ctagaagaag aactcaaacc tctggaggaa gtgctaaatt tagctcaaag caaaaacttt      900

cacttaagac ccagggactt aatcagcaat atcaacgtaa tagttctgga actaaaggga      960

tctgaaacaa cattcatgtg tgaatatgct gatgagacag caaccattgt agaatttctg     1020

aacagatgga ttaccttttg tcaaagcatc atctcaacac tgact                     1065


<210>  68
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-IL2 (C-terminal fusion)

<400>  68

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Met Leu Ser Gln Ala Pro 
    210                 215                 220                 


Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu 
225                 230                 235                 240 


Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro 
                245                 250                 255     


Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala 
            260                 265                 270         


Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu 
        275                 280                 285             


Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro 
    290                 295                 300                 


Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly 
305                 310                 315                 320 


Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile 
                325                 330                 335     


Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser 
            340                 345                 350         


Thr Leu Thr 
        355 


<210>  69
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-IL2 (N-terminal fusion)

<400>  69
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg catgctgagc      420

caggaagtcc aactggtcga aagcggtgga ggtctggtac agccaggagg aagtttgaga      480

ctgtcatgcg ctgcttccgg gtttactttc agcgactcct ggattcactg ggttcgtcag      540

gcacctggaa aggggcttga gtgggttgca tggatttccc catacggcgg ctctacatac      600

tacgctgaca gcgtcaaggg caggtttaca ataagcgccg atacctctaa aaacaccgcc      660

tacctgcaga tgaatagttt gcgagccgag gataccgccg tgtattattg tgcccggcgc      720

cattggcccg gcggcttcga ttattggggg caggggacac ttgtgacagt gtctgcagcc      780

tctaccaagg gccccagcgt gttccctctg gccccctgca gcagaagcac cagcgagtct      840

acagccgccc tgggatgcct ggtcaaggac tacttccccg agcccgtgac cgtgtcctgg      900

aactctggcg ccctgaccag cggcgtgcac acctttccag ccgtgctgca gagcagcggc      960

ctgtacagcc tgagcagcgt cgtgaccgtg cctagcagca gcctgggcac caagacctac     1020

acctgtaacg tggaccacaa gcccagcaac accaaggtgg acaagcgggt ggaatctaag     1080

tacggccctc cctgcccccc ctgcccagcc cctgaatttc tgggcggacc ctccgtgttc     1140

ctgttccccc caaagcccaa ggacaccctg atgatcagcc ggacccccga agtgacctgc     1200

gtggtggtgg acgtgtccca ggaagatccc gaggtccagt tcaactggta cgtggacggc     1260

gtggaagtgc acaacgccaa gaccaagccc agagaggaac agttcaacag cacctaccgg     1320

gtggtgtccg tgctgaccgt gctgcaccag gactggctga acggcaaaga gtacaagtgc     1380

aaggtctcca acaagggcct gcccagctcc atcgagaaaa ccatcagcaa ggccaagggc     1440

cagccccgcg agccccaggt gtacacactg ccccccagcc aggaagagat gaccaagaac     1500

caggtgtccc tgacctgcct cgtgaagggc ttctacccca gcgatatcgc cgtggaatgg     1560

gagagcaacg gccagcccga gaacaactac aagaccaccc cccctgtgct ggacagcgac     1620

ggcagcttct tcctgtactc ccggctgacc gtcgacaaga gccggtggca ggaaggcaac     1680

gtcttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg     1740

agcctgagcc tggga                                                      1755


<210>  70
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGMLSQ-IL2 (N-terminal fusion)

<400>  70

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Met Leu Ser Gln Glu Val Gln 
    130                 135                 140                 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile 
            180                 185                 190         


Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
        195                 200                 205             


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
225                 230                 235                 240 


His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
                245                 250                 255     


Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
            260                 265                 270         


Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val 
        275                 280                 285             


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
    290                 295                 300                 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
305                 310                 315                 320 


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
                325                 330                 335     


Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys 
            340                 345                 350         


Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys 
        355                 360                 365             


Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
    370                 375                 380                 


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
385                 390                 395                 400 


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
                405                 410                 415     


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
            420                 425                 430         


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
        435                 440                 445             


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
    450                 455                 460                 


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
465                 470                 475                 480 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
                485                 490                 495     


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
            500                 505                 510         


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
        515                 520                 525             


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
    530                 535                 540                 


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
545                 550                 555                 560 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
                565                 570                 575     


Gln Lys Ser Leu Ser Leu Ser Leu Gly 
            580                 585 


<210>  71
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-IL2 (N-terminal fusion)

<400>  71
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg catgctgagc      420

caggacatcc agatgaccca atccccatcc tccttgagcg catctgtagg agacagagtc      480

accatcacct gcagggcttc acaggatgtt agcacagctg tggcttggta ccagcagaaa      540

cctggaaagg ccccaaaact gctgatatac agcgcctcat tcctgtacag cggagtgccc      600

tctcgcttta gtggctctgg gagtggcact gatttcacac tgactatttc tagtctgcag      660

cccgaagatt tcgccactta ctattgccag cagtatctct atcaccccgc cacctttggg      720

cagggcacaa aggtcgagat taagcgtacg gtggccgctc ccagcgtgtt catcttccca      780

cccagcgacg agcagctgaa gtctggcacc gccagcgtcg tgtgcctgct gaacaacttc      840

tacccccgcg aggccaaggt gcagtggaag gtggacaacg ccctgcagtc cggcaacagc      900

caggaaagcg tcaccgagca ggacagcaag gactccacct acagcctgag cagcaccctg      960

accctgagca aggccgacta cgagaagcac aaggtgtacg cctgcgaagt gacccaccag     1020

ggcctgtcca gccccgtgac caagagcttc aaccggggcg agtgc                     1065


<210>  72
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGMLSQ-IL2 (N-terminal fusion)

<400>  72

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Met Leu Ser Gln Asp Ile Gln 
    130                 135                 140                 


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
145                 150                 155                 160 


Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala 
            180                 185                 190         


Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
    210                 215                 220                 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val 
                245                 250                 255     


Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser 
            260                 265                 270         


Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln 
        275                 280                 285             


Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val 
    290                 295                 300                 


Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu 
305                 310                 315                 320 


Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu 
                325                 330                 335     


Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg 
            340                 345                 350         


Gly Glu Cys 
        355 


<210>  73
<211>  1875
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -Nanoluc (C-terminal fusion)

<400>  73
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcattgcg ggccagctcg agatggtctt cacactcgaa     1380

gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag     1440

ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt     1500

gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt     1560

ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat     1620

gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg     1680

aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag     1740

atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac     1800

cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc     1860

gaacgcattc tggcg                                                      1875


<210>  74
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -Nanoluc (C-terminal fusion)

<400>  74

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Ile Ala Gly Gln Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly 
    450                 455                 460                 


Asp Trp Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln 
465                 470                 475                 480 


Gly Gly Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro 
                485                 490                 495     


Ile Gln Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile 
            500                 505                 510         


His Val Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln 
        515                 520                 525             


Ile Glu Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe 
    530                 535                 540                 


Lys Val Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro 
545                 550                 555                 560 


Asn Met Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe 
                565                 570                 575     


Asp Gly Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys 
            580                 585                 590         


Ile Ile Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg 
        595                 600                 605             


Val Thr Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu 
    610                 615                 620                 


Ala 
625 


<210>  75
<211>  1185
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -Nanoluc (C-terminal fusion)

<400>  75
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcattgcg      660

ggccagctcg agatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc      720

ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc      780

ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc      840

gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa      900

aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat      960

ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat     1020

gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc     1080

aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc     1140

atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcg                     1185


<210>  76
<211>  395
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -Nanoluc (C-terminal fusion)

<400>  76

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Ile Ala Gly Gln Leu Glu 
    210                 215                 220                 


Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
225                 230                 235                 240 


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
                245                 250                 255     


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
            260                 265                 270         


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
        275                 280                 285             


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
    290                 295                 300                 


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
305                 310                 315                 320 


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
                325                 330                 335     


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
            340                 345                 350         


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
        355                 360                 365             


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
    370                 375                 380                 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390                 395 


<210>  77
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -Nanoluc (N-terminal fusion)

<400>  77
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcattgc gggccaggaa      540

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      600

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      660

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      720

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      780

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      840

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      900

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      960

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct     1020

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac     1080

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1140

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1200

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1260

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1320

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1380

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1440

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1500

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1560

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1620

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1680

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1740

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1800

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1860

agcctggga                                                             1869


<210>  78
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -Nanoluc (N-terminal fusion)

<400>  78

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Ile 
                165                 170                 175     


Ala Gly Gln Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        195                 200                 205             


Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 
        275                 280                 285             


Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser 
    290                 295                 300                 


Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala 
305                 310                 315                 320 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                325                 330                 335     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            340                 345                 350         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        355                 360                 365             


Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His 
    370                 375                 380                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly 
385                 390                 395                 400 


Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser 
                405                 410                 415     


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            420                 425                 430         


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro 
        435                 440                 445             


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    450                 455                 460                 


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val 
465                 470                 475                 480 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                485                 490                 495     


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr 
            500                 505                 510         


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        515                 520                 525             


Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    530                 535                 540                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
545                 550                 555                 560 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
                565                 570                 575     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser 
            580                 585                 590         


Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        595                 600                 605             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620             


<210>  79
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -Nanoluc (N-terminal fusion)

<400>  79
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcattgc gggccaggac      540

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      600

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      660

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      720

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      780

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      840

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      900

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      960

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa     1020

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg     1080

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1140

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1179


<210>  80
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -Nanoluc (N-terminal fusion)

<400>  80

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Ile 
                165                 170                 175     


Ala Gly Gln Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            180                 185                 190         


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
        195                 200                 205             


Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
    210                 215                 220                 


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
225                 230                 235                 240 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                245                 250                 255     


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr 
            260                 265                 270         


His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
        275                 280                 285             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    290                 295                 300                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
305                 310                 315                 320 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                325                 330                 335     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            340                 345                 350         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        355                 360                 365             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    370                 375                 380                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
385                 390             


<210>  81
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -IL2 (C-terminal fusion)

<400>  81
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcattgcg ggccaggcac ctacctcgag ttctacaaag     1380

aaaacacagc tacaactgga gcatttactg ctggatttac agatgatttt gaatggaatt     1440

aataattaca agaatcccaa actcaccagg atgctcacat ttaagtttta catgcccaag     1500

aaggccacag aactgaaaca tcttcagtgt ctagaagaag aactcaaacc tctggaggaa     1560

gtgctaaatt tagctcaaag caaaaacttt cacttaagac ccagggactt aatcagcaat     1620

atcaacgtaa tagttctgga actaaaggga tctgaaacaa cattcatgtg tgaatatgct     1680

gatgagacag caaccattgt agaatttctg aacagatgga ttaccttttg tcaaagcatc     1740

atctcaacac tgact                                                      1755


<210>  82
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -IL2 (C-terminal fusion)

<400>  82

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Ile Ala Gly Gln Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
    450                 455                 460                 


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
465                 470                 475                 480 


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
                485                 490                 495     


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
            500                 505                 510         


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
        515                 520                 525             


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
    530                 535                 540                 


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
545                 550                 555                 560 


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
                565                 570                 575     


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585 


<210>  83
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -IL2 (C-terminal fusion)

<400>  83
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcattgcg      660

ggccaggcac ctacctcgag ttctacaaag aaaacacagc tacaactgga gcatttactg      720

ctggatttac agatgatttt gaatggaatt aataattaca agaatcccaa actcaccagg      780

atgctcacat ttaagtttta catgcccaag aaggccacag aactgaaaca tcttcagtgt      840

ctagaagaag aactcaaacc tctggaggaa gtgctaaatt tagctcaaag caaaaacttt      900

cacttaagac ccagggactt aatcagcaat atcaacgtaa tagttctgga actaaaggga      960

tctgaaacaa cattcatgtg tgaatatgct gatgagacag caaccattgt agaatttctg     1020

aacagatgga ttaccttttg tcaaagcatc atctcaacac tgact                     1065


<210>  84
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -IL2 (C-terminal fusion)

<400>  84

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Ile Ala Gly Gln Ala Pro 
    210                 215                 220                 


Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu 
225                 230                 235                 240 


Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro 
                245                 250                 255     


Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala 
            260                 265                 270         


Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu 
        275                 280                 285             


Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro 
    290                 295                 300                 


Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly 
305                 310                 315                 320 


Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile 
                325                 330                 335     


Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser 
            340                 345                 350         


Thr Leu Thr 
        355 


<210>  85
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -IL2 (N-terminal fusion)

<400>  85
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg cattgcgggc      420

caggaagtcc aactggtcga aagcggtgga ggtctggtac agccaggagg aagtttgaga      480

ctgtcatgcg ctgcttccgg gtttactttc agcgactcct ggattcactg ggttcgtcag      540

gcacctggaa aggggcttga gtgggttgca tggatttccc catacggcgg ctctacatac      600

tacgctgaca gcgtcaaggg caggtttaca ataagcgccg atacctctaa aaacaccgcc      660

tacctgcaga tgaatagttt gcgagccgag gataccgccg tgtattattg tgcccggcgc      720

cattggcccg gcggcttcga ttattggggg caggggacac ttgtgacagt gtctgcagcc      780

tctaccaagg gccccagcgt gttccctctg gccccctgca gcagaagcac cagcgagtct      840

acagccgccc tgggatgcct ggtcaaggac tacttccccg agcccgtgac cgtgtcctgg      900

aactctggcg ccctgaccag cggcgtgcac acctttccag ccgtgctgca gagcagcggc      960

ctgtacagcc tgagcagcgt cgtgaccgtg cctagcagca gcctgggcac caagacctac     1020

acctgtaacg tggaccacaa gcccagcaac accaaggtgg acaagcgggt ggaatctaag     1080

tacggccctc cctgcccccc ctgcccagcc cctgaatttc tgggcggacc ctccgtgttc     1140

ctgttccccc caaagcccaa ggacaccctg atgatcagcc ggacccccga agtgacctgc     1200

gtggtggtgg acgtgtccca ggaagatccc gaggtccagt tcaactggta cgtggacggc     1260

gtggaagtgc acaacgccaa gaccaagccc agagaggaac agttcaacag cacctaccgg     1320

gtggtgtccg tgctgaccgt gctgcaccag gactggctga acggcaaaga gtacaagtgc     1380

aaggtctcca acaagggcct gcccagctcc atcgagaaaa ccatcagcaa ggccaagggc     1440

cagccccgcg agccccaggt gtacacactg ccccccagcc aggaagagat gaccaagaac     1500

caggtgtccc tgacctgcct cgtgaagggc ttctacccca gcgatatcgc cgtggaatgg     1560

gagagcaacg gccagcccga gaacaactac aagaccaccc cccctgtgct ggacagcgac     1620

ggcagcttct tcctgtactc ccggctgacc gtcgacaaga gccggtggca ggaaggcaac     1680

gtcttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg     1740

agcctgagcc tggga                                                      1755


<210>  86
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGIAGQ -IL2 (N-terminal fusion)

<400>  86

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Ile Ala Gly Gln Glu Val Gln 
    130                 135                 140                 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile 
            180                 185                 190         


Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
        195                 200                 205             


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
225                 230                 235                 240 


His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
                245                 250                 255     


Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
            260                 265                 270         


Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val 
        275                 280                 285             


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
    290                 295                 300                 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
305                 310                 315                 320 


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
                325                 330                 335     


Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys 
            340                 345                 350         


Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys 
        355                 360                 365             


Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
    370                 375                 380                 


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
385                 390                 395                 400 


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
                405                 410                 415     


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
            420                 425                 430         


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
        435                 440                 445             


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
    450                 455                 460                 


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
465                 470                 475                 480 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
                485                 490                 495     


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
            500                 505                 510         


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
        515                 520                 525             


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
    530                 535                 540                 


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
545                 550                 555                 560 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
                565                 570                 575     


Gln Lys Ser Leu Ser Leu Ser Leu Gly 
            580                 585 


<210>  87
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -IL2 (N-terminal fusion)

<400>  87
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg cattgcgggc      420

caggacatcc agatgaccca atccccatcc tccttgagcg catctgtagg agacagagtc      480

accatcacct gcagggcttc acaggatgtt agcacagctg tggcttggta ccagcagaaa      540

cctggaaagg ccccaaaact gctgatatac agcgcctcat tcctgtacag cggagtgccc      600

tctcgcttta gtggctctgg gagtggcact gatttcacac tgactatttc tagtctgcag      660

cccgaagatt tcgccactta ctattgccag cagtatctct atcaccccgc cacctttggg      720

cagggcacaa aggtcgagat taagcgtacg gtggccgctc ccagcgtgtt catcttccca      780

cccagcgacg agcagctgaa gtctggcacc gccagcgtcg tgtgcctgct gaacaacttc      840

tacccccgcg aggccaaggt gcagtggaag gtggacaacg ccctgcagtc cggcaacagc      900

caggaaagcg tcaccgagca ggacagcaag gactccacct acagcctgag cagcaccctg      960

accctgagca aggccgacta cgagaagcac aaggtgtacg cctgcgaagt gacccaccag     1020

ggcctgtcca gccccgtgac caagagcttc aaccggggcg agtgc                     1065


<210>  88
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGIAGQ -IL2 (N-terminal fusion)

<400>  88

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Ile Ala Gly Gln Asp Ile Gln 
    130                 135                 140                 


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
145                 150                 155                 160 


Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala 
            180                 185                 190         


Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
    210                 215                 220                 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val 
                245                 250                 255     


Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser 
            260                 265                 270         


Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln 
        275                 280                 285             


Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val 
    290                 295                 300                 


Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu 
305                 310                 315                 320 


Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu 
                325                 330                 335     


Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg 
            340                 345                 350         


Gly Glu Cys 
        355 


<210>  89
<211>  1875
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -NanoLuc (C-terminal fusion)

<400>  89
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcctgtgg gcgcagctcg agatggtctt cacactcgaa     1380

gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag     1440

ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt     1500

gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt     1560

ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat     1620

gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg     1680

aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag     1740

atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac     1800

cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc     1860

gaacgcattc tggcg                                                      1875


<210>  90
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -NanoLuc (C-terminal fusion)

<400>  90

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Leu Trp Ala Gln Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly 
    450                 455                 460                 


Asp Trp Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln 
465                 470                 475                 480 


Gly Gly Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro 
                485                 490                 495     


Ile Gln Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile 
            500                 505                 510         


His Val Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln 
        515                 520                 525             


Ile Glu Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe 
    530                 535                 540                 


Lys Val Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro 
545                 550                 555                 560 


Asn Met Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe 
                565                 570                 575     


Asp Gly Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys 
            580                 585                 590         


Ile Ile Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg 
        595                 600                 605             


Val Thr Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu 
    610                 615                 620                 


Ala 
625 


<210>  91
<211>  1185
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -NanoLuc (C-terminal fusion)

<400>  91
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcctgtgg      660

gcgcagctcg agatggtctt cacactcgaa gatttcgttg gggactggcg acagacagcc      720

ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt tcagaatctc      780

ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg gctgaagatc      840

gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg ccagatcgaa      900

aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat cctgcactat      960

ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg acggccgtat     1020

gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct gtggaacggc     1080

aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt ccgagtaacc     1140

atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcg                     1185


<210>  92
<211>  395
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -NanoLuc (C-terminal fusion)

<400>  92

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Leu Trp Ala Gln Leu Glu 
    210                 215                 220                 


Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
225                 230                 235                 240 


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
                245                 250                 255     


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
            260                 265                 270         


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
        275                 280                 285             


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
    290                 295                 300                 


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
305                 310                 315                 320 


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
                325                 330                 335     


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
            340                 345                 350         


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
        355                 360                 365             


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
    370                 375                 380                 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390                 395 


<210>  93
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -NanoLuc (N-terminal fusion)

<400>  93
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcctgtg ggcgcaggaa      540

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      600

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      660

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      720

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      780

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      840

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      900

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      960

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct     1020

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac     1080

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1140

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1200

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1260

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1320

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1380

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1440

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1500

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1560

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1620

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1680

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1740

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1800

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1860

agcctggga                                                             1869


<210>  94
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -NanoLuc (N-terminal fusion)

<400>  94

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Leu 
                165                 170                 175     


Trp Ala Gln Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            180                 185                 190         


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        195                 200                 205             


Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    210                 215                 220                 


Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 
225                 230                 235                 240 


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
                245                 250                 255     


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            260                 265                 270         


Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 
        275                 280                 285             


Gln Gly Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser 
    290                 295                 300                 


Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala 
305                 310                 315                 320 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                325                 330                 335     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            340                 345                 350         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        355                 360                 365             


Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His 
    370                 375                 380                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly 
385                 390                 395                 400 


Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser 
                405                 410                 415     


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            420                 425                 430         


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro 
        435                 440                 445             


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    450                 455                 460                 


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val 
465                 470                 475                 480 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                485                 490                 495     


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr 
            500                 505                 510         


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        515                 520                 525             


Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    530                 535                 540                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
545                 550                 555                 560 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
                565                 570                 575     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser 
            580                 585                 590         


Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        595                 600                 605             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620             


<210>  95
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -NanoLuc (N-terminal fusion)

<400>  95
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcccgc tgggcctgtg ggcgcaggac      540

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      600

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      660

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      720

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      780

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      840

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      900

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      960

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa     1020

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg     1080

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1140

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1179


<210>  96
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -NanoLuc (N-terminal fusion)

<400>  96

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Pro Leu Gly Leu 
                165                 170                 175     


Trp Ala Gln Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            180                 185                 190         


Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val 
        195                 200                 205             


Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys 
    210                 215                 220                 


Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg 
225                 230                 235                 240 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                245                 250                 255     


Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr 
            260                 265                 270         


His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
        275                 280                 285             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    290                 295                 300                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
305                 310                 315                 320 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                325                 330                 335     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            340                 345                 350         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        355                 360                 365             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    370                 375                 380                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
385                 390             


<210>  97
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -IL2 (C-terminal fusion)

<400>  97
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcccgct gggcctgtgg gcgcaggcac ctacctcgag ttctacaaag     1380

aaaacacagc tacaactgga gcatttactg ctggatttac agatgatttt gaatggaatt     1440

aataattaca agaatcccaa actcaccagg atgctcacat ttaagtttta catgcccaag     1500

aaggccacag aactgaaaca tcttcagtgt ctagaagaag aactcaaacc tctggaggaa     1560

gtgctaaatt tagctcaaag caaaaacttt cacttaagac ccagggactt aatcagcaat     1620

atcaacgtaa tagttctgga actaaaggga tctgaaacaa cattcatgtg tgaatatgct     1680

gatgagacag caaccattgt agaatttctg aacagatgga ttaccttttg tcaaagcatc     1740

atctcaacac tgact                                                      1755


<210>  98
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -IL2 (C-terminal fusion)

<400>  98

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Pro Leu Gly 
        435                 440                 445             


Leu Trp Ala Gln Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
    450                 455                 460                 


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
465                 470                 475                 480 


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
                485                 490                 495     


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
            500                 505                 510         


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
        515                 520                 525             


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
    530                 535                 540                 


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
545                 550                 555                 560 


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
                565                 570                 575     


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
            580                 585 


<210>  99
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -IL2 (C-terminal fusion)

<400>  99
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcccgct gggcctgtgg      660

gcgcaggcac ctacctcgag ttctacaaag aaaacacagc tacaactgga gcatttactg      720

ctggatttac agatgatttt gaatggaatt aataattaca agaatcccaa actcaccagg      780

atgctcacat ttaagtttta catgcccaag aaggccacag aactgaaaca tcttcagtgt      840

ctagaagaag aactcaaacc tctggaggaa gtgctaaatt tagctcaaag caaaaacttt      900

cacttaagac ccagggactt aatcagcaat atcaacgtaa tagttctgga actaaaggga      960

tctgaaacaa cattcatgtg tgaatatgct gatgagacag caaccattgt agaatttctg     1020

aacagatgga ttaccttttg tcaaagcatc atctcaacac tgact                     1065


<210>  100
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -IL2 (C-terminal fusion)

<400>  100

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Pro Leu Gly Leu Trp Ala Gln Ala Pro 
    210                 215                 220                 


Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu 
225                 230                 235                 240 


Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro 
                245                 250                 255     


Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala 
            260                 265                 270         


Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu 
        275                 280                 285             


Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro 
    290                 295                 300                 


Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly 
305                 310                 315                 320 


Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile 
                325                 330                 335     


Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser 
            340                 345                 350         


Thr Leu Thr 
        355 


<210>  101
<211>  1755
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -IL2 (N-terminal fusion)

<400>  101
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg cctgtgggcg      420

caggaagtcc aactggtcga aagcggtgga ggtctggtac agccaggagg aagtttgaga      480

ctgtcatgcg ctgcttccgg gtttactttc agcgactcct ggattcactg ggttcgtcag      540

gcacctggaa aggggcttga gtgggttgca tggatttccc catacggcgg ctctacatac      600

tacgctgaca gcgtcaaggg caggtttaca ataagcgccg atacctctaa aaacaccgcc      660

tacctgcaga tgaatagttt gcgagccgag gataccgccg tgtattattg tgcccggcgc      720

cattggcccg gcggcttcga ttattggggg caggggacac ttgtgacagt gtctgcagcc      780

tctaccaagg gccccagcgt gttccctctg gccccctgca gcagaagcac cagcgagtct      840

acagccgccc tgggatgcct ggtcaaggac tacttccccg agcccgtgac cgtgtcctgg      900

aactctggcg ccctgaccag cggcgtgcac acctttccag ccgtgctgca gagcagcggc      960

ctgtacagcc tgagcagcgt cgtgaccgtg cctagcagca gcctgggcac caagacctac     1020

acctgtaacg tggaccacaa gcccagcaac accaaggtgg acaagcgggt ggaatctaag     1080

tacggccctc cctgcccccc ctgcccagcc cctgaatttc tgggcggacc ctccgtgttc     1140

ctgttccccc caaagcccaa ggacaccctg atgatcagcc ggacccccga agtgacctgc     1200

gtggtggtgg acgtgtccca ggaagatccc gaggtccagt tcaactggta cgtggacggc     1260

gtggaagtgc acaacgccaa gaccaagccc agagaggaac agttcaacag cacctaccgg     1320

gtggtgtccg tgctgaccgt gctgcaccag gactggctga acggcaaaga gtacaagtgc     1380

aaggtctcca acaagggcct gcccagctcc atcgagaaaa ccatcagcaa ggccaagggc     1440

cagccccgcg agccccaggt gtacacactg ccccccagcc aggaagagat gaccaagaac     1500

caggtgtccc tgacctgcct cgtgaagggc ttctacccca gcgatatcgc cgtggaatgg     1560

gagagcaacg gccagcccga gaacaactac aagaccaccc cccctgtgct ggacagcgac     1620

ggcagcttct tcctgtactc ccggctgacc gtcgacaaga gccggtggca ggaaggcaac     1680

gtcttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg     1740

agcctgagcc tggga                                                      1755


<210>  102
<211>  585
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GPLGLWAQ -IL2 (N-terminal fusion)

<400>  102

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Leu Trp Ala Gln Glu Val Gln 
    130                 135                 140                 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
145                 150                 155                 160 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His 
                165                 170                 175     


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile 
            180                 185                 190         


Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
        195                 200                 205             


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
    210                 215                 220                 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
225                 230                 235                 240 


His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
                245                 250                 255     


Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro 
            260                 265                 270         


Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val 
        275                 280                 285             


Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala 
    290                 295                 300                 


Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
305                 310                 315                 320 


Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly 
                325                 330                 335     


Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys 
            340                 345                 350         


Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys 
        355                 360                 365             


Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
    370                 375                 380                 


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
385                 390                 395                 400 


Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp 
                405                 410                 415     


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
            420                 425                 430         


Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
        435                 440                 445             


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
    450                 455                 460                 


Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
465                 470                 475                 480 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu 
                485                 490                 495     


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
            500                 505                 510         


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
        515                 520                 525             


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
    530                 535                 540                 


Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn 
545                 550                 555                 560 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
                565                 570                 575     


Gln Lys Ser Leu Ser Leu Ser Leu Gly 
            580                 585 


<210>  103
<211>  1065
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -IL2 (N-terminal fusion)

<400>  103
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcccgctggg cctgtgggcg      420

caggacatcc agatgaccca atccccatcc tccttgagcg catctgtagg agacagagtc      480

accatcacct gcagggcttc acaggatgtt agcacagctg tggcttggta ccagcagaaa      540

cctggaaagg ccccaaaact gctgatatac agcgcctcat tcctgtacag cggagtgccc      600

tctcgcttta gtggctctgg gagtggcact gatttcacac tgactatttc tagtctgcag      660

cccgaagatt tcgccactta ctattgccag cagtatctct atcaccccgc cacctttggg      720

cagggcacaa aggtcgagat taagcgtacg gtggccgctc ccagcgtgtt catcttccca      780

cccagcgacg agcagctgaa gtctggcacc gccagcgtcg tgtgcctgct gaacaacttc      840

tacccccgcg aggccaaggt gcagtggaag gtggacaacg ccctgcagtc cggcaacagc      900

caggaaagcg tcaccgagca ggacagcaag gactccacct acagcctgag cagcaccctg      960

accctgagca aggccgacta cgagaagcac aaggtgtacg cctgcgaagt gacccaccag     1020

ggcctgtcca gccccgtgac caagagcttc aaccggggcg agtgc                     1065


<210>  104
<211>  355
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GPLGLWAQ -IL2 (N-terminal fusion)

<400>  104

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Pro Leu Gly Leu Trp Ala Gln Asp Ile Gln 
    130                 135                 140                 


Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val 
145                 150                 155                 160 


Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala 
            180                 185                 190         


Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe 
    210                 215                 220                 


Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly 
225                 230                 235                 240 


Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val 
                245                 250                 255     


Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser 
            260                 265                 270         


Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln 
        275                 280                 285             


Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val 
    290                 295                 300                 


Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu 
305                 310                 315                 320 


Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu 
                325                 330                 335     


Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg 
            340                 345                 350         


Gly Glu Cys 
        355 


<210>  105
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG -NanoLuc (C-terminal fusion)

<400>  105
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaccggtggg cctgattggc ctcgagatgg tcttcacact cgaagatttc     1380

gttggggact ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt     1440

gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg     1500

agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc     1560

ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat     1620

cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg     1680

atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact     1740

gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac     1800

ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc     1860

attctggcg                                                             1869


<210>  106
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG -NanoLuc (C-terminal fusion)

<400>  106

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Pro Val Gly Leu 
        435                 440                 445             


Ile Gly Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp 
    450                 455                 460                 


Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly 
465                 470                 475                 480 


Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln 
                485                 490                 495     


Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val 
            500                 505                 510         


Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu 
        515                 520                 525             


Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val 
    530                 535                 540                 


Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met 
545                 550                 555                 560 


Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly 
                565                 570                 575     


Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile 
            580                 585                 590         


Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr 
        595                 600                 605             


Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
    610                 615                 620             


<210>  107
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG -NanoLuc (C-terminal fusion)

<400>  107
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcccggtggg cctgattggc      660

ctcgagatgg tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac      720

aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg      780

tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc      840

catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt      900

tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca      960

ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc     1020

atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa     1080

attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac     1140

ggagtgaccg gctggcggct gtgcgaacgc attctggcg                            1179


<210>  108
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG -NanoLuc (C-terminal fusion)

<400>  108

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Pro Val Gly Leu Ile Gly Leu Glu Met Val 
    210                 215                 220                 


Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr 
225                 230                 235                 240 


Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln 
                245                 250                 255     


Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly 
            260                 265                 270         


Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly 
        275                 280                 285             


Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val 
    290                 295                 300                 


Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly Thr 
305                 310                 315                 320 


Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg 
                325                 330                 335     


Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr 
            340                 345                 350         


Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn 
        355                 360                 365             


Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly 
    370                 375                 380                 


Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390             


<210>  109
<211>  1863
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG -NanoLuc (N-terminal fusion)

<400>  109
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgccggtgg gcctgattgg cgaagtccaa      540

ctggtcgaaa gcggtggagg tctggtacag ccaggaggaa gtttgagact gtcatgcgct      600

gcttccgggt ttactttcag cgactcctgg attcactggg ttcgtcaggc acctggaaag      660

gggcttgagt gggttgcatg gatttcccca tacggcggct ctacatacta cgctgacagc      720

gtcaagggca ggtttacaat aagcgccgat acctctaaaa acaccgccta cctgcagatg      780

aatagtttgc gagccgagga taccgccgtg tattattgtg cccggcgcca ttggcccggc      840

ggcttcgatt attgggggca ggggacactt gtgacagtgt ctgcagcctc taccaagggc      900

cccagcgtgt tccctctggc cccctgcagc agaagcacca gcgagtctac agccgccctg      960

ggatgcctgg tcaaggacta cttccccgag cccgtgaccg tgtcctggaa ctctggcgcc     1020

ctgaccagcg gcgtgcacac ctttccagcc gtgctgcaga gcagcggcct gtacagcctg     1080

agcagcgtcg tgaccgtgcc tagcagcagc ctgggcacca agacctacac ctgtaacgtg     1140

gaccacaagc ccagcaacac caaggtggac aagcgggtgg aatctaagta cggccctccc     1200

tgccccccct gcccagcccc tgaatttctg ggcggaccct ccgtgttcct gttcccccca     1260

aagcccaagg acaccctgat gatcagccgg acccccgaag tgacctgcgt ggtggtggac     1320

gtgtcccagg aagatcccga ggtccagttc aactggtacg tggacggcgt ggaagtgcac     1380

aacgccaaga ccaagcccag agaggaacag ttcaacagca cctaccgggt ggtgtccgtg     1440

ctgaccgtgc tgcaccagga ctggctgaac ggcaaagagt acaagtgcaa ggtctccaac     1500

aagggcctgc ccagctccat cgagaaaacc atcagcaagg ccaagggcca gccccgcgag     1560

ccccaggtgt acacactgcc ccccagccag gaagagatga ccaagaacca ggtgtccctg     1620

acctgcctcg tgaagggctt ctaccccagc gatatcgccg tggaatggga gagcaacggc     1680

cagcccgaga acaactacaa gaccaccccc cctgtgctgg acagcgacgg cagcttcttc     1740

ctgtactccc ggctgaccgt cgacaagagc cggtggcagg aaggcaacgt cttcagctgc     1800

agcgtgatgc acgaggccct gcacaaccac tacacccaga agtccctgag cctgagcctg     1860

gga                                                                   1863


<210>  110
<211>  621
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG -NanoLuc (N-terminal fusion)

<400>  110

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Pro Val Gly Leu Ile 
                165                 170                 175     


Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
            180                 185                 190         


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp 
        195                 200                 205             


Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
    210                 215                 220                 


Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser 
225                 230                 235                 240 


Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala 
                245                 250                 255     


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
            260                 265                 270         


Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly 
        275                 280                 285             


Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe 
    290                 295                 300                 


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
305                 310                 315                 320 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                325                 330                 335     


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
            340                 345                 350         


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
        355                 360                 365             


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
    370                 375                 380                 


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro 
385                 390                 395                 400 


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe 
                405                 410                 415     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            420                 425                 430         


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
        435                 440                 445             


Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    450                 455                 460                 


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
465                 470                 475                 480 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                485                 490                 495     


Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
            500                 505                 510         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        515                 520                 525             


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    530                 535                 540                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
545                 550                 555                 560 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                565                 570                 575     


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
            580                 585                 590         


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        595                 600                 605             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620     


<210>  111
<211>  1173
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG -NanoLuc (N-terminal fusion)

<400>  111
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgccggtgg gcctgattgg cgacatccag      540

atgacccaat ccccatcctc cttgagcgca tctgtaggag acagagtcac catcacctgc      600

agggcttcac aggatgttag cacagctgtg gcttggtacc agcagaaacc tggaaaggcc      660

ccaaaactgc tgatatacag cgcctcattc ctgtacagcg gagtgccctc tcgctttagt      720

ggctctggga gtggcactga tttcacactg actatttcta gtctgcagcc cgaagatttc      780

gccacttact attgccagca gtatctctat caccccgcca cctttgggca gggcacaaag      840

gtcgagatta agcgtacggt ggccgctccc agcgtgttca tcttcccacc cagcgacgag      900

cagctgaagt ctggcaccgc cagcgtcgtg tgcctgctga acaacttcta cccccgcgag      960

gccaaggtgc agtggaaggt ggacaacgcc ctgcagtccg gcaacagcca ggaaagcgtc     1020

accgagcagg acagcaagga ctccacctac agcctgagca gcaccctgac cctgagcaag     1080

gccgactacg agaagcacaa ggtgtacgcc tgcgaagtga cccaccaggg cctgtccagc     1140

cccgtgacca agagcttcaa ccggggcgag tgc                                  1173


<210>  112
<211>  391
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG -NanoLuc (N-terminal fusion)

<400>  112

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Pro Val Gly Leu Ile 
                165                 170                 175     


Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            180                 185                 190         


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr 
        195                 200                 205             


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
    210                 215                 220                 


Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser 
225                 230                 235                 240 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
                245                 250                 255     


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro 
            260                 265                 270         


Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
        275                 280                 285             


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
    290                 295                 300                 


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
305                 310                 315                 320 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
                325                 330                 335     


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
            340                 345                 350         


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
        355                 360                 365             


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
    370                 375                 380                 


Ser Phe Asn Arg Gly Glu Cys 
385                 390     


<210>  113
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG-IL2 (C-terminal fusion)

<400>  113
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaccggtggg cctgattggc gcacctacct cgagttctac aaagaaaaca     1380

cagctacaac tggagcattt actgctggat ttacagatga ttttgaatgg aattaataat     1440

tacaagaatc ccaaactcac caggatgctc acatttaagt tttacatgcc caagaaggcc     1500

acagaactga aacatcttca gtgtctagaa gaagaactca aacctctgga ggaagtgcta     1560

aatttagctc aaagcaaaaa ctttcactta agacccaggg acttaatcag caatatcaac     1620

gtaatagttc tggaactaaa gggatctgaa acaacattca tgtgtgaata tgctgatgag     1680

acagcaacca ttgtagaatt tctgaacaga tggattacct tttgtcaaag catcatctca     1740

acactgact                                                             1749


<210>  114
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG-IL2 (C-terminal fusion)

<400>  114

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Pro Val Gly Leu 
        435                 440                 445             


Ile Gly Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu 
    450                 455                 460                 


Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn 
465                 470                 475                 480 


Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met 
                485                 490                 495     


Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu 
            500                 505                 510         


Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe 
        515                 520                 525             


His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu 
    530                 535                 540                 


Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu 
545                 550                 555                 560 


Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln 
                565                 570                 575     


Ser Ile Ile Ser Thr Leu Thr 
            580             


<210>  115
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG-IL2 (C-terminal fusion)

<400>  115
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcccggtggg cctgattggc      660

gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat      720

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      780

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      840

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      900

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      960

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga     1020

tggattacct tttgtcaaag catcatctca acactgact                            1059


<210>  116
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG-IL2 (C-terminal fusion)

<400>  116

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Pro Val Gly Leu Ile Gly Ala Pro Thr Ser 
    210                 215                 220                 


Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp 
225                 230                 235                 240 


Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu 
                245                 250                 255     


Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu 
            260                 265                 270         


Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp 
    290                 295                 300                 


Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu 
305                 310                 315                 320 


Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu 
                325                 330                 335     


Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser Thr Leu 
            340                 345                 350         


Thr 
    


<210>  117
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG-IL2 (N-terminal fusion)

<400>  117
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactc cggtgggcct gattggcgaa      420

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      480

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      540

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      600

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      660

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      720

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      780

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      840

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct      900

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac      960

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1020

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1080

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1140

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1200

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1260

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1320

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1380

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1440

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1500

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1560

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1620

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1680

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1740

agcctggga                                                             1749


<210>  118
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PVGLIG-IL2 (N-terminal fusion)

<400>  118

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Val Gly Leu Ile Gly Glu Val Gln Leu Val 
    130                 135                 140                 


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
145                 150                 155                 160 


Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val 
                165                 170                 175     


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro 
            180                 185                 190         


Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
        195                 200                 205             


Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser 
    210                 215                 220                 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp 
225                 230                 235                 240 


Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250                 255     


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser 
            260                 265                 270         


Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
        275                 280                 285             


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
    290                 295                 300                 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
305                 310                 315                 320 


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys 
                325                 330                 335     


Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp 
            340                 345                 350         


Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala 
        355                 360                 365             


Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    370                 375                 380                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
385                 390                 395                 400 


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
                405                 410                 415     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            420                 425                 430         


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        435                 440                 445             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
    450                 455                 460                 


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
465                 470                 475                 480 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
                485                 490                 495     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            500                 505                 510         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        515                 520                 525             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    530                 535                 540                 


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
545                 550                 555                 560 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                565                 570                 575     


Ser Leu Ser Leu Ser Leu Gly 
            580             


<210>  119
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG-IL2 (N-terminal fusion)

<400>  119
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactc cggtgggcct gattggcgac      420

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      480

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      540

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      600

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      660

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      720

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      780

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      840

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa      900

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg      960

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1020

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1059


<210>  120
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PVGLIG-IL2 (N-terminal fusion)

<400>  120

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Val Gly Leu Ile Gly Asp Ile Gln Met Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
            180                 185                 190         


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
                245                 250                 255     


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
            260                 265                 270         


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
        275                 280                 285             


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
    290                 295                 300                 


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
305                 310                 315                 320 


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
                325                 330                 335     


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
            340                 345                 350         


Cys 
    


<210>  121
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-NanoLuc (C-terminal fusion)

<400>  121
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaccgctggg cctggcgggc ctcgagatgg tcttcacact cgaagatttc     1380

gttggggact ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt     1440

gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg     1500

agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc     1560

ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat     1620

cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg     1680

atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact     1740

gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac     1800

ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc     1860

attctggcg                                                             1869


<210>  122
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-NanoLuc (C-terminal fusion)

<400>  122

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Pro Leu Gly Leu 
        435                 440                 445             


Ala Gly Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp 
    450                 455                 460                 


Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly 
465                 470                 475                 480 


Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln 
                485                 490                 495     


Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val 
            500                 505                 510         


Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu 
        515                 520                 525             


Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val 
    530                 535                 540                 


Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met 
545                 550                 555                 560 


Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly 
                565                 570                 575     


Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile 
            580                 585                 590         


Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr 
        595                 600                 605             


Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
    610                 615                 620             


<210>  123
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-NanoLuc (C-terminal fusion)

<400>  123
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcccgctggg cctggcgggc      660

ctcgagatgg tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac      720

aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg      780

tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc      840

catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt      900

tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca      960

ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc     1020

atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa     1080

attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac     1140

ggagtgaccg gctggcggct gtgcgaacgc attctggcg                            1179


<210>  124
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-NanoLuc (C-terminal fusion)

<400>  124

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Pro Leu Gly Leu Ala Gly Leu Glu Met Val 
    210                 215                 220                 


Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr 
225                 230                 235                 240 


Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln 
                245                 250                 255     


Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly 
            260                 265                 270         


Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly 
        275                 280                 285             


Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val 
    290                 295                 300                 


Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly Thr 
305                 310                 315                 320 


Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg 
                325                 330                 335     


Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr 
            340                 345                 350         


Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn 
        355                 360                 365             


Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly 
    370                 375                 380                 


Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390             


<210>  125
<211>  1863
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-NanoLuc (N-terminal fusion)

<400>  125
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgccgctgg gcctggcggg cgaagtccaa      540

ctggtcgaaa gcggtggagg tctggtacag ccaggaggaa gtttgagact gtcatgcgct      600

gcttccgggt ttactttcag cgactcctgg attcactggg ttcgtcaggc acctggaaag      660

gggcttgagt gggttgcatg gatttcccca tacggcggct ctacatacta cgctgacagc      720

gtcaagggca ggtttacaat aagcgccgat acctctaaaa acaccgccta cctgcagatg      780

aatagtttgc gagccgagga taccgccgtg tattattgtg cccggcgcca ttggcccggc      840

ggcttcgatt attgggggca ggggacactt gtgacagtgt ctgcagcctc taccaagggc      900

cccagcgtgt tccctctggc cccctgcagc agaagcacca gcgagtctac agccgccctg      960

ggatgcctgg tcaaggacta cttccccgag cccgtgaccg tgtcctggaa ctctggcgcc     1020

ctgaccagcg gcgtgcacac ctttccagcc gtgctgcaga gcagcggcct gtacagcctg     1080

agcagcgtcg tgaccgtgcc tagcagcagc ctgggcacca agacctacac ctgtaacgtg     1140

gaccacaagc ccagcaacac caaggtggac aagcgggtgg aatctaagta cggccctccc     1200

tgccccccct gcccagcccc tgaatttctg ggcggaccct ccgtgttcct gttcccccca     1260

aagcccaagg acaccctgat gatcagccgg acccccgaag tgacctgcgt ggtggtggac     1320

gtgtcccagg aagatcccga ggtccagttc aactggtacg tggacggcgt ggaagtgcac     1380

aacgccaaga ccaagcccag agaggaacag ttcaacagca cctaccgggt ggtgtccgtg     1440

ctgaccgtgc tgcaccagga ctggctgaac ggcaaagagt acaagtgcaa ggtctccaac     1500

aagggcctgc ccagctccat cgagaaaacc atcagcaagg ccaagggcca gccccgcgag     1560

ccccaggtgt acacactgcc ccccagccag gaagagatga ccaagaacca ggtgtccctg     1620

acctgcctcg tgaagggctt ctaccccagc gatatcgccg tggaatggga gagcaacggc     1680

cagcccgaga acaactacaa gaccaccccc cctgtgctgg acagcgacgg cagcttcttc     1740

ctgtactccc ggctgaccgt cgacaagagc cggtggcagg aaggcaacgt cttcagctgc     1800

agcgtgatgc acgaggccct gcacaaccac tacacccaga agtccctgag cctgagcctg     1860

gga                                                                   1863


<210>  126
<211>  621
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-NanoLuc (N-terminal fusion)

<400>  126

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Pro Leu Gly Leu Ala 
                165                 170                 175     


Gly Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
            180                 185                 190         


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp 
        195                 200                 205             


Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
    210                 215                 220                 


Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser 
225                 230                 235                 240 


Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala 
                245                 250                 255     


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
            260                 265                 270         


Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly 
        275                 280                 285             


Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe 
    290                 295                 300                 


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
305                 310                 315                 320 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                325                 330                 335     


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
            340                 345                 350         


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
        355                 360                 365             


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
    370                 375                 380                 


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro 
385                 390                 395                 400 


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe 
                405                 410                 415     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            420                 425                 430         


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
        435                 440                 445             


Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    450                 455                 460                 


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
465                 470                 475                 480 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                485                 490                 495     


Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
            500                 505                 510         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        515                 520                 525             


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    530                 535                 540                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
545                 550                 555                 560 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                565                 570                 575     


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
            580                 585                 590         


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        595                 600                 605             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620     


<210>  127
<211>  1173
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-NanoLuc (N-terminal fusion)

<400>  127
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgccgctgg gcctggcggg cgacatccag      540

atgacccaat ccccatcctc cttgagcgca tctgtaggag acagagtcac catcacctgc      600

agggcttcac aggatgttag cacagctgtg gcttggtacc agcagaaacc tggaaaggcc      660

ccaaaactgc tgatatacag cgcctcattc ctgtacagcg gagtgccctc tcgctttagt      720

ggctctggga gtggcactga tttcacactg actatttcta gtctgcagcc cgaagatttc      780

gccacttact attgccagca gtatctctat caccccgcca cctttgggca gggcacaaag      840

gtcgagatta agcgtacggt ggccgctccc agcgtgttca tcttcccacc cagcgacgag      900

cagctgaagt ctggcaccgc cagcgtcgtg tgcctgctga acaacttcta cccccgcgag      960

gccaaggtgc agtggaaggt ggacaacgcc ctgcagtccg gcaacagcca ggaaagcgtc     1020

accgagcagg acagcaagga ctccacctac agcctgagca gcaccctgac cctgagcaag     1080

gccgactacg agaagcacaa ggtgtacgcc tgcgaagtga cccaccaggg cctgtccagc     1140

cccgtgacca agagcttcaa ccggggcgag tgc                                  1173


<210>  128
<211>  391
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-NanoLuc (N-terminal fusion)

<400>  128

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Pro Leu Gly Leu Ala 
                165                 170                 175     


Gly Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            180                 185                 190         


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr 
        195                 200                 205             


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
    210                 215                 220                 


Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser 
225                 230                 235                 240 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
                245                 250                 255     


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro 
            260                 265                 270         


Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
        275                 280                 285             


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
    290                 295                 300                 


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
305                 310                 315                 320 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
                325                 330                 335     


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
            340                 345                 350         


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
        355                 360                 365             


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
    370                 375                 380                 


Ser Phe Asn Arg Gly Glu Cys 
385                 390     


<210>  129
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-IL2 (C-terminal fusion)

<400>  129
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaccgctggg cctggcgggc gcacctacct cgagttctac aaagaaaaca     1380

cagctacaac tggagcattt actgctggat ttacagatga ttttgaatgg aattaataat     1440

tacaagaatc ccaaactcac caggatgctc acatttaagt tttacatgcc caagaaggcc     1500

acagaactga aacatcttca gtgtctagaa gaagaactca aacctctgga ggaagtgcta     1560

aatttagctc aaagcaaaaa ctttcactta agacccaggg acttaatcag caatatcaac     1620

gtaatagttc tggaactaaa gggatctgaa acaacattca tgtgtgaata tgctgatgag     1680

acagcaacca ttgtagaatt tctgaacaga tggattacct tttgtcaaag catcatctca     1740

acactgact                                                             1749


<210>  130
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-IL2 (C-terminal fusion)

<400>  130

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Pro Leu Gly Leu 
        435                 440                 445             


Ala Gly Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu 
    450                 455                 460                 


Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn 
465                 470                 475                 480 


Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met 
                485                 490                 495     


Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu 
            500                 505                 510         


Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe 
        515                 520                 525             


His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu 
    530                 535                 540                 


Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu 
545                 550                 555                 560 


Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln 
                565                 570                 575     


Ser Ile Ile Ser Thr Leu Thr 
            580             


<210>  131
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-IL2 (C-terminal fusion)

<400>  131
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcccgctggg cctggcgggc      660

gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat      720

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      780

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      840

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      900

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      960

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga     1020

tggattacct tttgtcaaag catcatctca acactgact                            1059


<210>  132
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-IL2 (C-terminal fusion)

<400>  132

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Pro Leu Gly Leu Ala Gly Ala Pro Thr Ser 
    210                 215                 220                 


Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp 
225                 230                 235                 240 


Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu 
                245                 250                 255     


Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu 
            260                 265                 270         


Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp 
    290                 295                 300                 


Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu 
305                 310                 315                 320 


Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu 
                325                 330                 335     


Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser Thr Leu 
            340                 345                 350         


Thr 
    


<210>  133
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-IL2 (N-terminal fusion)

<400>  133
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactc cgctgggcct ggcgggcgaa      420

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      480

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      540

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      600

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      660

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      720

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      780

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      840

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct      900

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac      960

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1020

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1080

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1140

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1200

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1260

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1320

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1380

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1440

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1500

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1560

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1620

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1680

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1740

agcctggga                                                             1749


<210>  134
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-PLGLAG-IL2 (N-terminal fusion)

<400>  134

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Leu Gly Leu Ala Gly Glu Val Gln Leu Val 
    130                 135                 140                 


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
145                 150                 155                 160 


Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val 
                165                 170                 175     


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro 
            180                 185                 190         


Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
        195                 200                 205             


Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser 
    210                 215                 220                 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp 
225                 230                 235                 240 


Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250                 255     


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser 
            260                 265                 270         


Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
        275                 280                 285             


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
    290                 295                 300                 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
305                 310                 315                 320 


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys 
                325                 330                 335     


Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp 
            340                 345                 350         


Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala 
        355                 360                 365             


Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    370                 375                 380                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
385                 390                 395                 400 


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
                405                 410                 415     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            420                 425                 430         


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        435                 440                 445             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
    450                 455                 460                 


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
465                 470                 475                 480 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
                485                 490                 495     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            500                 505                 510         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        515                 520                 525             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    530                 535                 540                 


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
545                 550                 555                 560 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                565                 570                 575     


Ser Leu Ser Leu Ser Leu Gly 
            580             


<210>  135
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-IL2 (N-terminal fusion)

<400>  135
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactc cgctgggcct ggcgggcgac      420

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      480

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      540

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      600

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      660

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      720

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      780

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      840

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa      900

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg      960

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1020

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1059


<210>  136
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-PLGLAG-IL2 (N-terminal fusion)

<400>  136

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Pro Leu Gly Leu Ala Gly Asp Ile Gln Met Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
            180                 185                 190         


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
                245                 250                 255     


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
            260                 265                 270         


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
        275                 280                 285             


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
    290                 295                 300                 


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
305                 310                 315                 320 


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
                325                 330                 335     


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
            340                 345                 350         


Cys 
    


<210>  137
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-NanoLuc (C-terminal fusion)

<400>  137
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcattgt gggaccgctg ctcgagatgg tcttcacact cgaagatttc     1380

gttggggact ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt     1440

gtgtccagtt tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg     1500

agcggtgaaa atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc     1560

ggcgaccaaa tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat     1620

cactttaagg tgatcctgca ctatggcaca ctggtaatcg acggggttac gccgaacatg     1680

atcgactatt tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact     1740

gtaacaggga ccctgtggaa cggcaacaaa attatcgacg agcgcctgat caaccccgac     1800

ggctccctgc tgttccgagt aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc     1860

attctggcg                                                             1869


<210>  138
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-NanoLuc (C-terminal fusion)

<400>  138

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Ile Val Gly 
        435                 440                 445             


Pro Leu Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp 
    450                 455                 460                 


Arg Gln Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly 
465                 470                 475                 480 


Val Ser Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln 
                485                 490                 495     


Arg Ile Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val 
            500                 505                 510         


Ile Ile Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu 
        515                 520                 525             


Lys Ile Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val 
    530                 535                 540                 


Ile Leu His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met 
545                 550                 555                 560 


Ile Asp Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly 
                565                 570                 575     


Lys Lys Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile 
            580                 585                 590         


Asp Glu Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr 
        595                 600                 605             


Ile Asn Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
    610                 615                 620             


<210>  139
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-NanoLuc (C-terminal fusion)

<400>  139
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcattgt gggaccgctg      660

ctcgagatgg tcttcacact cgaagatttc gttggggact ggcgacagac agccggctac      720

aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa tctcggggtg      780

tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa gatcgacatc      840

catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat cgaaaaaatt      900

tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca ctatggcaca      960

ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc gtatgaaggc     1020

atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa cggcaacaaa     1080

attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt aaccatcaac     1140

ggagtgaccg gctggcggct gtgcgaacgc attctggcg                            1179


<210>  140
<211>  393
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-NanoLuc (C-terminal fusion)

<400>  140

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Ile Val Gly Pro Leu Leu Glu Met Val 
    210                 215                 220                 


Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala Gly Tyr 
225                 230                 235                 240 


Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Phe Gln 
                245                 250                 255     


Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu Ser Gly 
            260                 265                 270         


Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly 
        275                 280                 285             


Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys Val Val 
    290                 295                 300                 


Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr Gly Thr 
305                 310                 315                 320 


Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly Arg 
                325                 330                 335     


Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr 
            340                 345                 350         


Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Asn 
        355                 360                 365             


Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr Gly 
    370                 375                 380                 


Trp Arg Leu Cys Glu Arg Ile Leu Ala 
385                 390             


<210>  141
<211>  1863
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-NanoLuc (N-terminal fusion)

<400>  141
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcattg tgggaccgct ggaagtccaa      540

ctggtcgaaa gcggtggagg tctggtacag ccaggaggaa gtttgagact gtcatgcgct      600

gcttccgggt ttactttcag cgactcctgg attcactggg ttcgtcaggc acctggaaag      660

gggcttgagt gggttgcatg gatttcccca tacggcggct ctacatacta cgctgacagc      720

gtcaagggca ggtttacaat aagcgccgat acctctaaaa acaccgccta cctgcagatg      780

aatagtttgc gagccgagga taccgccgtg tattattgtg cccggcgcca ttggcccggc      840

ggcttcgatt attgggggca ggggacactt gtgacagtgt ctgcagcctc taccaagggc      900

cccagcgtgt tccctctggc cccctgcagc agaagcacca gcgagtctac agccgccctg      960

ggatgcctgg tcaaggacta cttccccgag cccgtgaccg tgtcctggaa ctctggcgcc     1020

ctgaccagcg gcgtgcacac ctttccagcc gtgctgcaga gcagcggcct gtacagcctg     1080

agcagcgtcg tgaccgtgcc tagcagcagc ctgggcacca agacctacac ctgtaacgtg     1140

gaccacaagc ccagcaacac caaggtggac aagcgggtgg aatctaagta cggccctccc     1200

tgccccccct gcccagcccc tgaatttctg ggcggaccct ccgtgttcct gttcccccca     1260

aagcccaagg acaccctgat gatcagccgg acccccgaag tgacctgcgt ggtggtggac     1320

gtgtcccagg aagatcccga ggtccagttc aactggtacg tggacggcgt ggaagtgcac     1380

aacgccaaga ccaagcccag agaggaacag ttcaacagca cctaccgggt ggtgtccgtg     1440

ctgaccgtgc tgcaccagga ctggctgaac ggcaaagagt acaagtgcaa ggtctccaac     1500

aagggcctgc ccagctccat cgagaaaacc atcagcaagg ccaagggcca gccccgcgag     1560

ccccaggtgt acacactgcc ccccagccag gaagagatga ccaagaacca ggtgtccctg     1620

acctgcctcg tgaagggctt ctaccccagc gatatcgccg tggaatggga gagcaacggc     1680

cagcccgaga acaactacaa gaccaccccc cctgtgctgg acagcgacgg cagcttcttc     1740

ctgtactccc ggctgaccgt cgacaagagc cggtggcagg aaggcaacgt cttcagctgc     1800

agcgtgatgc acgaggccct gcacaaccac tacacccaga agtccctgag cctgagcctg     1860

gga                                                                   1863


<210>  142
<211>  621
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-NanoLuc (N-terminal fusion)

<400>  142

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Ile Val Gly Pro 
                165                 170                 175     


Leu Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
            180                 185                 190         


Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp 
        195                 200                 205             


Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
    210                 215                 220                 


Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser 
225                 230                 235                 240 


Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala 
                245                 250                 255     


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
            260                 265                 270         


Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly 
        275                 280                 285             


Thr Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe 
    290                 295                 300                 


Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu 
305                 310                 315                 320 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                325                 330                 335     


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
            340                 345                 350         


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
        355                 360                 365             


Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro 
    370                 375                 380                 


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro 
385                 390                 395                 400 


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe 
                405                 410                 415     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            420                 425                 430         


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val 
        435                 440                 445             


Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    450                 455                 460                 


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 
465                 470                 475                 480 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                485                 490                 495     


Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser 
            500                 505                 510         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        515                 520                 525             


Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    530                 535                 540                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
545                 550                 555                 560 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                565                 570                 575     


Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp 
            580                 585                 590         


Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        595                 600                 605             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
    610                 615                 620     


<210>  143
<211>  1173
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-NanoLuc (N-terminal fusion)

<400>  143
atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg       60

gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta      120

actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc      180

atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag      240

gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta      300

atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc      360

gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc      420

gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg      480

accggctggc ggctgtgcga acgcattctg gcgggcattg tgggaccgct ggacatccag      540

atgacccaat ccccatcctc cttgagcgca tctgtaggag acagagtcac catcacctgc      600

agggcttcac aggatgttag cacagctgtg gcttggtacc agcagaaacc tggaaaggcc      660

ccaaaactgc tgatatacag cgcctcattc ctgtacagcg gagtgccctc tcgctttagt      720

ggctctggga gtggcactga tttcacactg actatttcta gtctgcagcc cgaagatttc      780

gccacttact attgccagca gtatctctat caccccgcca cctttgggca gggcacaaag      840

gtcgagatta agcgtacggt ggccgctccc agcgtgttca tcttcccacc cagcgacgag      900

cagctgaagt ctggcaccgc cagcgtcgtg tgcctgctga acaacttcta cccccgcgag      960

gccaaggtgc agtggaaggt ggacaacgcc ctgcagtccg gcaacagcca ggaaagcgtc     1020

accgagcagg acagcaagga ctccacctac agcctgagca gcaccctgac cctgagcaag     1080

gccgactacg agaagcacaa ggtgtacgcc tgcgaagtga cccaccaggg cctgtccagc     1140

cccgtgacca agagcttcaa ccggggcgag tgc                                  1173


<210>  144
<211>  391
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-NanoLuc (N-terminal fusion)

<400>  144

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Ile Val Gly Pro 
                165                 170                 175     


Leu Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
            180                 185                 190         


Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr 
        195                 200                 205             


Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
    210                 215                 220                 


Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser 
225                 230                 235                 240 


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
                245                 250                 255     


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro 
            260                 265                 270         


Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala 
        275                 280                 285             


Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser 
    290                 295                 300                 


Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu 
305                 310                 315                 320 


Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser 
                325                 330                 335     


Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu 
            340                 345                 350         


Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val 
        355                 360                 365             


Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys 
    370                 375                 380                 


Ser Phe Asn Arg Gly Glu Cys 
385                 390     


<210>  145
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-IL2 (C-terminal fusion)

<400>  145
gaagtccaac tggtcgaaag cggtggaggt ctggtacagc caggaggaag tttgagactg       60

tcatgcgctg cttccgggtt tactttcagc gactcctgga ttcactgggt tcgtcaggca      120

cctggaaagg ggcttgagtg ggttgcatgg atttccccat acggcggctc tacatactac      180

gctgacagcg tcaagggcag gtttacaata agcgccgata cctctaaaaa caccgcctac      240

ctgcagatga atagtttgcg agccgaggat accgccgtgt attattgtgc ccggcgccat      300

tggcccggcg gcttcgatta ttgggggcag gggacacttg tgacagtgtc tgcagcctct      360

accaagggcc ccagcgtgtt ccctctggcc ccctgcagca gaagcaccag cgagtctaca      420

gccgccctgg gatgcctggt caaggactac ttccccgagc ccgtgaccgt gtcctggaac      480

tctggcgccc tgaccagcgg cgtgcacacc tttccagccg tgctgcagag cagcggcctg      540

tacagcctga gcagcgtcgt gaccgtgcct agcagcagcc tgggcaccaa gacctacacc      600

tgtaacgtgg accacaagcc cagcaacacc aaggtggaca agcgggtgga atctaagtac      660

ggccctccct gccccccctg cccagcccct gaatttctgg gcggaccctc cgtgttcctg      720

ttccccccaa agcccaagga caccctgatg atcagccgga cccccgaagt gacctgcgtg      780

gtggtggacg tgtcccagga agatcccgag gtccagttca actggtacgt ggacggcgtg      840

gaagtgcaca acgccaagac caagcccaga gaggaacagt tcaacagcac ctaccgggtg      900

gtgtccgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagagta caagtgcaag      960

gtctccaaca agggcctgcc cagctccatc gagaaaacca tcagcaaggc caagggccag     1020

ccccgcgagc cccaggtgta cacactgccc cccagccagg aagagatgac caagaaccag     1080

gtgtccctga cctgcctcgt gaagggcttc taccccagcg atatcgccgt ggaatgggag     1140

agcaacggcc agcccgagaa caactacaag accacccccc ctgtgctgga cagcgacggc     1200

agcttcttcc tgtactcccg gctgaccgtc gacaagagcc ggtggcagga aggcaacgtc     1260

ttcagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gtccctgagc     1320

ctgagcctgg gaggcattgt gggaccgctg gcacctacct cgagttctac aaagaaaaca     1380

cagctacaac tggagcattt actgctggat ttacagatga ttttgaatgg aattaataat     1440

tacaagaatc ccaaactcac caggatgctc acatttaagt tttacatgcc caagaaggcc     1500

acagaactga aacatcttca gtgtctagaa gaagaactca aacctctgga ggaagtgcta     1560

aatttagctc aaagcaaaaa ctttcactta agacccaggg acttaatcag caatatcaac     1620

gtaatagttc tggaactaaa gggatctgaa acaacattca tgtgtgaata tgctgatgag     1680

acagcaacca ttgtagaatt tctgaacaga tggattacct tttgtcaaag catcatctca     1740

acactgact                                                             1749


<210>  146
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-IL2 (C-terminal fusion)

<400>  146

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          


Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Ile Val Gly 
        435                 440                 445             


Pro Leu Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu 
    450                 455                 460                 


Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn 
465                 470                 475                 480 


Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met 
                485                 490                 495     


Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu 
            500                 505                 510         


Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe 
        515                 520                 525             


His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu 
    530                 535                 540                 


Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu 
545                 550                 555                 560 


Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln 
                565                 570                 575     


Ser Ile Ile Ser Thr Leu Thr 
            580             


<210>  147
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-IL2 (C-terminal fusion)

<400>  147
gacatccaga tgacccaatc cccatcctcc ttgagcgcat ctgtaggaga cagagtcacc       60

atcacctgca gggcttcaca ggatgttagc acagctgtgg cttggtacca gcagaaacct      120

ggaaaggccc caaaactgct gatatacagc gcctcattcc tgtacagcgg agtgccctct      180

cgctttagtg gctctgggag tggcactgat ttcacactga ctatttctag tctgcagccc      240

gaagatttcg ccacttacta ttgccagcag tatctctatc accccgccac ctttgggcag      300

ggcacaaagg tcgagattaa gcgtacggtg gccgctccca gcgtgttcat cttcccaccc      360

agcgacgagc agctgaagtc tggcaccgcc agcgtcgtgt gcctgctgaa caacttctac      420

ccccgcgagg ccaaggtgca gtggaaggtg gacaacgccc tgcagtccgg caacagccag      480

gaaagcgtca ccgagcagga cagcaaggac tccacctaca gcctgagcag caccctgacc      540

ctgagcaagg ccgactacga gaagcacaag gtgtacgcct gcgaagtgac ccaccagggc      600

ctgtccagcc ccgtgaccaa gagcttcaac cggggcgagt gcggcattgt gggaccgctg      660

gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat      720

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      780

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      840

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      900

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      960

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga     1020

tggattacct tttgtcaaag catcatctca acactgact                            1059


<210>  148
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-IL2 (C-terminal fusion)

<400>  148

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys Gly Ile Val Gly Pro Leu Ala Pro Thr Ser 
    210                 215                 220                 


Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu Leu Asp 
225                 230                 235                 240 


Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro Lys Leu 
                245                 250                 255     


Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu 
            260                 265                 270         


Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp 
    290                 295                 300                 


Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu 
305                 310                 315                 320 


Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu 
                325                 330                 335     


Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser Thr Leu 
            340                 345                 350         


Thr 
    


<210>  149
<211>  1749
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-IL2 (N-terminal fusion)

<400>  149
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcattgtggg accgctggaa      420

gtccaactgg tcgaaagcgg tggaggtctg gtacagccag gaggaagttt gagactgtca      480

tgcgctgctt ccgggtttac tttcagcgac tcctggattc actgggttcg tcaggcacct      540

ggaaaggggc ttgagtgggt tgcatggatt tccccatacg gcggctctac atactacgct      600

gacagcgtca agggcaggtt tacaataagc gccgatacct ctaaaaacac cgcctacctg      660

cagatgaata gtttgcgagc cgaggatacc gccgtgtatt attgtgcccg gcgccattgg      720

cccggcggct tcgattattg ggggcagggg acacttgtga cagtgtctgc agcctctacc      780

aagggcccca gcgtgttccc tctggccccc tgcagcagaa gcaccagcga gtctacagcc      840

gccctgggat gcctggtcaa ggactacttc cccgagcccg tgaccgtgtc ctggaactct      900

ggcgccctga ccagcggcgt gcacaccttt ccagccgtgc tgcagagcag cggcctgtac      960

agcctgagca gcgtcgtgac cgtgcctagc agcagcctgg gcaccaagac ctacacctgt     1020

aacgtggacc acaagcccag caacaccaag gtggacaagc gggtggaatc taagtacggc     1080

cctccctgcc ccccctgccc agcccctgaa tttctgggcg gaccctccgt gttcctgttc     1140

cccccaaagc ccaaggacac cctgatgatc agccggaccc ccgaagtgac ctgcgtggtg     1200

gtggacgtgt cccaggaaga tcccgaggtc cagttcaact ggtacgtgga cggcgtggaa     1260

gtgcacaacg ccaagaccaa gcccagagag gaacagttca acagcaccta ccgggtggtg     1320

tccgtgctga ccgtgctgca ccaggactgg ctgaacggca aagagtacaa gtgcaaggtc     1380

tccaacaagg gcctgcccag ctccatcgag aaaaccatca gcaaggccaa gggccagccc     1440

cgcgagcccc aggtgtacac actgcccccc agccaggaag agatgaccaa gaaccaggtg     1500

tccctgacct gcctcgtgaa gggcttctac cccagcgata tcgccgtgga atgggagagc     1560

aacggccagc ccgagaacaa ctacaagacc accccccctg tgctggacag cgacggcagc     1620

ttcttcctgt actcccggct gaccgtcgac aagagccggt ggcaggaagg caacgtcttc     1680

agctgcagcg tgatgcacga ggccctgcac aaccactaca cccagaagtc cctgagcctg     1740

agcctggga                                                             1749


<210>  150
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of PD-L1-GIVGPL-IL2 (N-terminal fusion)

<400>  150

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Ile Val Gly Pro Leu Glu Val Gln Leu Val 
    130                 135                 140                 


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
145                 150                 155                 160 


Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val 
                165                 170                 175     


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro 
            180                 185                 190         


Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
        195                 200                 205             


Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser 
    210                 215                 220                 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp 
225                 230                 235                 240 


Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
                245                 250                 255     


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser 
            260                 265                 270         


Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
        275                 280                 285             


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
    290                 295                 300                 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
305                 310                 315                 320 


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys 
                325                 330                 335     


Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp 
            340                 345                 350         


Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala 
        355                 360                 365             


Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
    370                 375                 380                 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
385                 390                 395                 400 


Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val 
                405                 410                 415     


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
            420                 425                 430         


Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
        435                 440                 445             


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly 
    450                 455                 460                 


Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
465                 470                 475                 480 


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr 
                485                 490                 495     


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
            500                 505                 510         


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
        515                 520                 525             


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
    530                 535                 540                 


Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe 
545                 550                 555                 560 


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
                565                 570                 575     


Ser Leu Ser Leu Ser Leu Gly 
            580             


<210>  151
<211>  1059
<212>  DNA
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-IL2 (N-terminal fusion)

<400>  151
gcacctacct cgagttctac aaagaaaaca cagctacaac tggagcattt actgctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttgtcaaag catcatctca acactgactg gcattgtggg accgctggac      420

atccagatga cccaatcccc atcctccttg agcgcatctg taggagacag agtcaccatc      480

acctgcaggg cttcacagga tgttagcaca gctgtggctt ggtaccagca gaaacctgga      540

aaggccccaa aactgctgat atacagcgcc tcattcctgt acagcggagt gccctctcgc      600

tttagtggct ctgggagtgg cactgatttc acactgacta tttctagtct gcagcccgaa      660

gatttcgcca cttactattg ccagcagtat ctctatcacc ccgccacctt tgggcagggc      720

acaaaggtcg agattaagcg tacggtggcc gctcccagcg tgttcatctt cccacccagc      780

gacgagcagc tgaagtctgg caccgccagc gtcgtgtgcc tgctgaacaa cttctacccc      840

cgcgaggcca aggtgcagtg gaaggtggac aacgccctgc agtccggcaa cagccaggaa      900

agcgtcaccg agcaggacag caaggactcc acctacagcc tgagcagcac cctgaccctg      960

agcaaggccg actacgagaa gcacaaggtg tacgcctgcg aagtgaccca ccagggcctg     1020

tccagccccg tgaccaagag cttcaaccgg ggcgagtgc                            1059


<210>  152
<211>  353
<212>  PRT
<213>  Artificial

<220>
<223>  light chain of PD-L1-GIVGPL-IL2 (N-terminal fusion)

<400>  152

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr Gly Ile Val Gly Pro Leu Asp Ile Gln Met Thr 
    130                 135                 140                 


Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
145                 150                 155                 160 


Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln 
                165                 170                 175     


Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe 
            180                 185                 190         


Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             


Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
    210                 215                 220                 


Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly 
225                 230                 235                 240 


Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile 
                245                 250                 255     


Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val 
            260                 265                 270         


Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys 
        275                 280                 285             


Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu 
    290                 295                 300                 


Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu 
305                 310                 315                 320 


Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr 
                325                 330                 335     


His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu 
            340                 345                 350         


Cys 
    


<210>  153
<211>  1863
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-NanoLuc (C-terminal fusion)

<400>  153
gaagtgatgt tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtacag cctctggatt cactttcagt acctatgaca tgtcttgggt tcgccagact      120

ccggagaaga ggctggagtg ggtctcaacc attagtagta gtcgtactta cacctactat      180

tcagccagtg tgaaggggcg attcaccatc tccagagaca atgccaggaa catcctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaattt      300

tctcactggg gccaagggac tctggtcact gtctctgcag cgtccacaaa gggcccaagc      360

gtgttcccgc tagcccccag cagcaagagc accagcggcg gcacagccgc cctgggctgc      420

ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg agccctgacc      480

tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag cctgagcagc      540

gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa cgtgaaccac      600

aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga caagacccac      660

acctgtcccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt cctgttcccc      720

cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg tgtggtggtg      780

gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg cgtggaggtg      840

cacaacgcca agaccaagcc cagagaggag cagtacaaca gcacctacag ggtggtgtcc      900

gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg taaggtgtcc      960

aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg ccagccaaga     1020

gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa ccaggtgtcc     1080

ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg ggagagcaac     1140

ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga cggcagcttc     1200

ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa cgtgttcagc     1260

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct gagcctgtcc     1320

ccaggcccgg tgggcctgat tggcctcgag atggtcttca cactcgaaga tttcgttggg     1380

gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc     1440

agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt     1500

gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac     1560

caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt     1620

aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac     1680

tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca     1740

gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc     1800

ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg     1860

gcg                                                                   1863


<210>  154
<211>  621
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-NanoLuc (C-terminal fusion)

<400>  154

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
    210                 215                 220                 


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val Gly Leu Ile Gly 
        435                 440                 445             


Leu Glu Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln 
    450                 455                 460                 


Thr Ala Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser 
465                 470                 475                 480 


Ser Leu Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile 
                485                 490                 495     


Val Leu Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile 
            500                 505                 510         


Pro Tyr Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile 
        515                 520                 525             


Phe Lys Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu 
    530                 535                 540                 


His Tyr Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp 
545                 550                 555                 560 


Tyr Phe Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys 
                565                 570                 575     


Ile Thr Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu 
            580                 585                 590         


Arg Leu Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
        595                 600                 605             


Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
    610                 615                 620     


<210>  155
<211>  1551
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-CCL4 (C-terminal fusion)

<400>  155
gaagtgatgt tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtacag cctctggatt cactttcagt acctatgaca tgtcttgggt tcgccagact      120

ccggagaaga ggctggagtg ggtctcaacc attagtagta gtcgtactta cacctactat      180

tcagccagtg tgaaggggcg attcaccatc tccagagaca atgccaggaa catcctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaattt      300

tctcactggg gccaagggac tctggtcact gtctctgcag cgtccacaaa gggcccaagc      360

gtgttcccgc tagcccccag cagcaagagc accagcggcg gcacagccgc cctgggctgc      420

ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg agccctgacc      480

tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag cctgagcagc      540

gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa cgtgaaccac      600

aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga caagacccac      660

acctgtcccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt cctgttcccc      720

cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg tgtggtggtg      780

gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg cgtggaggtg      840

cacaacgcca agaccaagcc cagagaggag cagtacaaca gcacctacag ggtggtgtcc      900

gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg taaggtgtcc      960

aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg ccagccaaga     1020

gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa ccaggtgtcc     1080

ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg ggagagcaac     1140

ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga cggcagcttc     1200

ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa cgtgttcagc     1260

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct gagcctgtcc     1320

ccaggcccgg tgggcctgat tggcgcacca atgggctcag accctcccac cgcctgctgc     1380

ttttcttaca ccgcgaggaa acttcctcgc aactttgtgg tagattacta tgagaccagc     1440

agcctctgct cccagccagc tgtggtattc caaaccaaaa gaagcaagca agtctgtgct     1500

gatcccagtg aatcctgggt ccaggagtac gtgtatgacc tggaactgaa c              1551


<210>  156
<211>  517
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-CCL4 (C-terminal fusion)

<400>  156

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
    210                 215                 220                 


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val Gly Leu Ile Gly 
        435                 440                 445             


Ala Pro Met Gly Ser Asp Pro Pro Thr Ala Cys Cys Phe Ser Tyr Thr 
    450                 455                 460                 


Ala Arg Lys Leu Pro Arg Asn Phe Val Val Asp Tyr Tyr Glu Thr Ser 
465                 470                 475                 480 


Ser Leu Cys Ser Gln Pro Ala Val Val Phe Gln Thr Lys Arg Ser Lys 
                485                 490                 495     


Gln Val Cys Ala Asp Pro Ser Glu Ser Trp Val Gln Glu Tyr Val Tyr 
            500                 505                 510         


Asp Leu Glu Leu Asn 
        515         


<210>  157
<211>  1686
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-IL15 (C-terminal fusion)

<400>  157
gaagtgatgt tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtacag cctctggatt cactttcagt acctatgaca tgtcttgggt tcgccagact      120

ccggagaaga ggctggagtg ggtctcaacc attagtagta gtcgtactta cacctactat      180

tcagccagtg tgaaggggcg attcaccatc tccagagaca atgccaggaa catcctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaattt      300

tctcactggg gccaagggac tctggtcact gtctctgcag cgtccacaaa gggcccaagc      360

gtgttcccgc tagcccccag cagcaagagc accagcggcg gcacagccgc cctgggctgc      420

ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg agccctgacc      480

tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag cctgagcagc      540

gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa cgtgaaccac      600

aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga caagacccac      660

acctgtcccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt cctgttcccc      720

cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg tgtggtggtg      780

gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg cgtggaggtg      840

cacaacgcca agaccaagcc cagagaggag cagtacaaca gcacctacag ggtggtgtcc      900

gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg taaggtgtcc      960

aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg ccagccaaga     1020

gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa ccaggtgtcc     1080

ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg ggagagcaac     1140

ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga cggcagcttc     1200

ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa cgtgttcagc     1260

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct gagcctgtcc     1320

ccaggcccgg tgggcctgat tggcaactgg gtgaatgtaa taagtgattt gaaaaaaatt     1380

gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac     1440

cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt     1500

gagtccggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac     1560

agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag     1620

gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac     1680

acttct                                                                1686


<210>  158
<211>  562
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-IL15 (C-terminal fusion)

<400>  158

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
    210                 215                 220                 


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val Gly Leu Ile Gly 
        435                 440                 445             


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    450                 455                 460                 


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
465                 470                 475                 480 


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                485                 490                 495     


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            500                 505                 510         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        515                 520                 525             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    530                 535                 540                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
545                 550                 555                 560 


Thr Ser 
        


<210>  159
<211>  1839
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-IFNalpha (C-terminal fusion)

<400>  159
gaagtgatgt tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtacag cctctggatt cactttcagt acctatgaca tgtcttgggt tcgccagact      120

ccggagaaga ggctggagtg ggtctcaacc attagtagta gtcgtactta cacctactat      180

tcagccagtg tgaaggggcg attcaccatc tccagagaca atgccaggaa catcctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaattt      300

tctcactggg gccaagggac tctggtcact gtctctgcag cgtccacaaa gggcccaagc      360

gtgttcccgc tagcccccag cagcaagagc accagcggcg gcacagccgc cctgggctgc      420

ctggtgaagg actacttccc cgagcccgtg accgtgtcct ggaacagcgg agccctgacc      480

tccggcgtgc acaccttccc cgccgtgctg cagagcagcg gcctgtacag cctgagcagc      540

gtggtgaccg tgcccagcag cagcctgggc acccagacct acatctgtaa cgtgaaccac      600

aagcccagca acaccaaggt ggacaagaga gtggagccca agagctgtga caagacccac      660

acctgtcccc cctgcccagc ccccgagctg ctgggcggac ccagcgtgtt cctgttcccc      720

cccaagccca aggacaccct gatgatcagc agaacccccg aggtgacctg tgtggtggtg      780

gacgtgtccc acgaggaccc agaggtgaag ttcaactggt acgtggacgg cgtggaggtg      840

cacaacgcca agaccaagcc cagagaggag cagtacaaca gcacctacag ggtggtgtcc      900

gtgctgaccg tgctgcacca ggactggctg aacggcaagg agtacaagtg taaggtgtcc      960

aacaaggccc tgccagcccc aatcgaaaag accatcagca aggccaaggg ccagccaaga     1020

gagccccagg tgtacaccct gccacccagc agggaggaga tgaccaagaa ccaggtgtcc     1080

ctgacctgtc tggtgaaggg cttctaccca agcgacatcg ccgtggagtg ggagagcaac     1140

ggccagcccg agaacaacta caagaccacc cccccagtgc tggacagcga cggcagcttc     1200

ttcctgtaca gcaagctgac cgtggacaag agcagatggc agcagggcaa cgtgttcagc     1260

tgctccgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct gagcctgtcc     1320

ccaggcccgg tgggcctgat tggctgtgat ctgcctcaaa cccacagcct gggtagcagg     1380

aggaccttga tgctcctggc acagatgagg aaaatctctc ttttctcctg cttgaaggac     1440

agacatgact ttggatttcc ccaggaggag tttggcaacc agttccaaaa ggctgaaacc     1500

atccctgtcc tccatgagat gatccagcag atcttcaatc tcttcagcac aaaggactca     1560

tctgctgctt gggatgagac cctcctagac aaattctaca ctgaactcta ccagcagctg     1620

aatgacctgg aagcctgtgt gatacagggg gtgggggtga cagagactcc cctgatgaag     1680

gaggactcca ttctggctgt gaggaaatac ttccaaagaa tcactctcta tctgaaagag     1740

aagaaataca gcccttgtgc ctgggaggtt gtcagagcag aaatcatgag atctttttct     1800

ttgtcaacaa acttgcaaga aagtttaaga agtaaggaa                            1839


<210>  160
<211>  613
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of 9G4-PVGLIG-IFNalpha (C-terminal fusion)

<400>  160

Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser Ala Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
        115                 120                 125             


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
    130                 135                 140                 


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
145                 150                 155                 160 


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
                165                 170                 175     


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
            180                 185                 190         


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
        195                 200                 205             


Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro 
    210                 215                 220                 


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
225                 230                 235                 240 


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
            260                 265                 270         


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
        275                 280                 285             


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
    290                 295                 300                 


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
305                 310                 315                 320 


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
                325                 330                 335     


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu 
            340                 345                 350         


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
    370                 375                 380                 


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
                405                 410                 415     


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
            420                 425                 430         


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val Gly Leu Ile Gly 
        435                 440                 445             


Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met 
    450                 455                 460                 


Leu Leu Ala Gln Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp 
465                 470                 475                 480 


Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln 
                485                 490                 495     


Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe 
            500                 505                 510         


Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu 
        515                 520                 525             


Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu 
    530                 535                 540                 


Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys 
545                 550                 555                 560 


Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu 
                565                 570                 575     


Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg 
            580                 585                 590         


Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser 
        595                 600                 605             


Leu Arg Ser Lys Glu 
    610             


<210>  161
<211>  1698
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of NHS76-PVGLIG-IL15 (C-terminal fusion)

<400>  161
caggtgcagc tgcaggagtc cggcccagga ctggtgaagc cttcggagac cctgtccctc       60

acctgcgctg tctctggtta ctccatcagc agtggttact actggggctg gattcggcag      120

cccccaggga aggggctgga gtggattggg agtatctatc atagtgggag cacctactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca cgtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcagac acggccgtgt attactgtgc aagagggaag      300

tggtcgaagt ttgactattg gggccaaggc accctggtca ccgtctcttc agcgtccaca      360

aagggcccaa gcgtgttccc gctagccccc agcagcaaga gcaccagcgg cggcacagcc      420

gccctgggct gcctggtgaa ggactacttc cccgagcccg tgaccgtgtc ctggaacagc      480

ggagccctga cctccggcgt gcacaccttc cccgccgtgc tgcagagcag cggcctgtac      540

agcctgagca gcgtggtgac cgtgcccagc agcagcctgg gcacccagac ctacatctgt      600

aacgtgaacc acaagcccag caacaccaag gtggacaaga gagtggagcc caagagctgt      660

gacaagaccc acacctgtcc cccctgccca gcccccgagc tgctgggcgg acccagcgtg      720

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      780

tgtgtggtgg tggacgtgtc ccacgaggac ccagaggtga agttcaactg gtacgtggac      840

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagtacaa cagcacctac      900

agggtggtgt ccgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag      960

tgtaaggtgt ccaacaaggc cctgccagcc ccaatcgaaa agaccatcag caaggccaag     1020

ggccagccaa gagagcccca ggtgtacacc ctgccaccca gcagggagga gatgaccaag     1080

aaccaggtgt ccctgacctg tctggtgaag ggcttctacc caagcgacat cgccgtggag     1140

tgggagagca acggccagcc cgagaacaac tacaagacca cccccccagt gctggacagc     1200

gacggcagct tcttcctgta cagcaagctg accgtggaca agagcagatg gcagcagggc     1260

aacgtgttca gctgctccgt gatgcacgag gccctgcaca accactacac ccagaagagc     1320

ctgagcctgt ccccaggccc ggtgggcctg attggcaact gggtgaatgt aataagtgat     1380

ttgaaaaaaa ttgaagatct tattcaatct atgcatattg atgctacttt atatacggaa     1440

agtgatgttc accccagttg caaagtaaca gcaatgaagt gctttctctt ggagttacaa     1500

gttatttcac ttgagtccgg agatgcaagt attcatgata cagtagaaaa tctgatcatc     1560

ctagcaaaca acagtttgtc ttctaatggg aatgtaacag aatctggatg caaagaatgt     1620

gaggaactgg aggaaaaaaa tattaaagaa tttttgcaga gttttgtaca tattgtccaa     1680

atgttcatca acacttct                                                   1698


<210>  162
<211>  566
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of NHS76-PVGLIG-IL15 (C-terminal fusion)

<400>  162

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Glu 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Tyr Ser Ile Ser Ser Gly 
            20                  25                  30          


Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ser Ile Tyr His Ser Gly Ser Thr Tyr Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Lys Trp Ser Lys Phe Asp Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Pro Val 
        435                 440                 445             


Gly Leu Ile Gly Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile 
    450                 455                 460                 


Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu 
465                 470                 475                 480 


Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu 
                485                 490                 495     


Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His 
            500                 505                 510         


Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser 
        515                 520                 525             


Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu 
    530                 535                 540                 


Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln 
545                 550                 555                 560 


Met Phe Ile Asn Thr Ser 
                565     


<210>  163
<211>  1704
<212>  DNA
<213>  Artificial

<220>
<223>  heavy chain of H16/L16-PVGLIG-IL15 (C-terminal fusion)

<400>  163
caggtgcagc tgcaggagtc gggcccagga ctggtgaagc cttcggggac cctgtccctc       60

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      120

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      180

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      240

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      300

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      360

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      420

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      480

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      540

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      600

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      660

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      720

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      780

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      840

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      900

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag      960

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1020

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1080

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1140

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1200

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1260

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1320

aagagcctga gcctgtcccc aggcccggtg ggcctgattg gcaactgggt gaatgtaata     1380

agtgatttga aaaaaattga agatcttatt caatctatgc atattgatgc tactttatat     1440

acggaaagtg atgttcaccc cagttgcaaa gtaacagcaa tgaagtgctt tctcttggag     1500

ttacaagtta tttcacttga gtccggagat gcaagtattc atgatacagt agaaaatctg     1560

atcatcctag caaacaacag tttgtcttct aatgggaatg taacagaatc tggatgcaaa     1620

gaatgtgagg aactggagga aaaaaatatt aaagaatttt tgcagagttt tgtacatatt     1680

gtccaaatgt tcatcaacac ttct                                            1704


<210>  164
<211>  568
<212>  PRT
<213>  Artificial

<220>
<223>  heavy chain of H16/L16-PVGLIG-IL15 (C-terminal fusion)

<400>  164

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gly 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser Ile Ser Ser Ser 
            20                  25                  30          


Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu 
    50                  55                  60                  


Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys Asn Gln Phe Ser 
65                  70                  75                  80  


Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile Trp Gly Gln Gly 
            100                 105                 110         


Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
225                 230                 235                 240 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
                245                 250                 255     


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
            260                 265                 270         


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
        275                 280                 285             


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
    290                 295                 300                 


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
305                 310                 315                 320 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
                325                 330                 335     


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            340                 345                 350         


Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        355                 360                 365             


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    370                 375                 380                 


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
385                 390                 395                 400 


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
                405                 410                 415     


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            420                 425                 430         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


Pro Val Gly Leu Ile Gly Asn Trp Val Asn Val Ile Ser Asp Leu Lys 
    450                 455                 460                 


Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr 
465                 470                 475                 480 


Thr Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys 
                485                 490                 495     


Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser 
            500                 505                 510         


Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu 
        515                 520                 525             


Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu 
    530                 535                 540                 


Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile 
545                 550                 555                 560 


Val Gln Met Phe Ile Asn Thr Ser 
                565             


<210>  165
<211>  12
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  165
tcaggcagat ct                                                           12


<210>  166
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  166

Ser Gly Arg Ser 
1               


<210>  167
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  167
tcaggcagat ctgcc                                                        15


<210>  168
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  168

Ser Gly Arg Ser Ala 
1               5   


<210>  169
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  linker

<400>  169
ccgagcagcc ggagaagagt gaac                                              24


<210>  170
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  linker

<400>  170

Pro Ser Ser Arg Arg Arg Val Asn 
1               5               


<210>  171
<211>  447
<212>  DNA
<213>  Homo sapiens

<400>  171
aaacccatca ccggcaccat caacgacctg aaccagcaag tgtggacact gcagggccag       60

aatctggtgg ccgtgcctag aagcgatagc gtgacacctg tgaccgtggc cgtgatcaca      120

tgcaagtacc ccgaggctct tgagcaaggc agaggcgatc ctatctacct gggcattcag      180

aaccccgaga tgtgcctgta ctgcgagaaa gtgggagagc agcccacact gcagctgaaa      240

gaacagaaaa tcatggacct gtacggccag cctgagcctg tgaagccctt cctgttttac      300

agagccaaga ccggccggac cagcacactg gaatctgtgg cctttcctga ctggtttatc      360

gccagcagca agcgggacca gcctatcatc ctgacaagcg agctgggcaa gagctacaac      420

accgccttcg agctgaacat caacgat                                          447


<210>  172
<211>  149
<212>  PRT
<213>  Homo sapiens

<400>  172

Lys Pro Ile Thr Gly Thr Ile Asn Asp Leu Asn Gln Gln Val Trp Thr 
1               5                   10                  15      


Leu Gln Gly Gln Asn Leu Val Ala Val Pro Arg Ser Asp Ser Val Thr 
            20                  25                  30          


Pro Val Thr Val Ala Val Ile Thr Cys Lys Tyr Pro Glu Ala Leu Glu 
        35                  40                  45              


Gln Gly Arg Gly Asp Pro Ile Tyr Leu Gly Ile Gln Asn Pro Glu Met 
    50                  55                  60                  


Cys Leu Tyr Cys Glu Lys Val Gly Glu Gln Pro Thr Leu Gln Leu Lys 
65                  70                  75                  80  


Glu Gln Lys Ile Met Asp Leu Tyr Gly Gln Pro Glu Pro Val Lys Pro 
                85                  90                  95      


Phe Leu Phe Tyr Arg Ala Lys Thr Gly Arg Thr Ser Thr Leu Glu Ser 
            100                 105                 110         


Val Ala Phe Pro Asp Trp Phe Ile Ala Ser Ser Lys Arg Asp Gln Pro 
        115                 120                 125             


Ile Ile Leu Thr Ser Glu Leu Gly Lys Ser Tyr Asn Thr Ala Phe Glu 
    130                 135                 140                 


Leu Asn Ile Asn Asp 
145                 


<210>  173
<211>  1869
<212>  DNA
<213>  Artificial

<220>
<223>  H16/L16-PVGLIG-IL36G heavy chain

<400>  173
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc      120

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      180

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      360

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      420

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      480

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      540

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      600

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      660

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      720

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      780

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      840

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      900

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      960

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag     1020

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1080

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1140

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1200

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1260

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1320

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1380

aagagcctga gcctgtcccc aggcccggtg ggcctgattg gcaaacccat caccggcacc     1440

atcaacgacc tgaaccagca agtgtggaca ctgcagggcc agaatctggt ggccgtgcct     1500

agaagcgata gcgtgacacc tgtgaccgtg gccgtgatca catgcaagta ccccgaggct     1560

cttgagcaag gcagaggcga tcctatctac ctgggcattc agaaccccga gatgtgcctg     1620

tactgcgaga aagtgggaga gcagcccaca ctgcagctga aagaacagaa aatcatggac     1680

ctgtacggcc agcctgagcc tgtgaagccc ttcctgtttt acagagccaa gaccggccgg     1740

accagcacac tggaatctgt ggcctttcct gactggttta tcgccagcag caagcgggac     1800

cagcctatca tcctgacaag cgagctgggc aagagctaca acaccgcctt cgagctgaac     1860

atcaacgat                                                             1869


<210>  174
<211>  623
<212>  PRT
<213>  Artificial

<220>
<223>  H16/L16-PVGLIG-IL36G heavy chain

<400>  174

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gly Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser 
        35                  40                  45              


Ile Ser Ser Ser Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Pro Val Gly Leu Ile Gly Lys Pro Ile Thr Gly Thr 
465                 470                 475                 480 


Ile Asn Asp Leu Asn Gln Gln Val Trp Thr Leu Gln Gly Gln Asn Leu 
                485                 490                 495     


Val Ala Val Pro Arg Ser Asp Ser Val Thr Pro Val Thr Val Ala Val 
            500                 505                 510         


Ile Thr Cys Lys Tyr Pro Glu Ala Leu Glu Gln Gly Arg Gly Asp Pro 
        515                 520                 525             


Ile Tyr Leu Gly Ile Gln Asn Pro Glu Met Cys Leu Tyr Cys Glu Lys 
    530                 535                 540                 


Val Gly Glu Gln Pro Thr Leu Gln Leu Lys Glu Gln Lys Ile Met Asp 
545                 550                 555                 560 


Leu Tyr Gly Gln Pro Glu Pro Val Lys Pro Phe Leu Phe Tyr Arg Ala 
                565                 570                 575     


Lys Thr Gly Arg Thr Ser Thr Leu Glu Ser Val Ala Phe Pro Asp Trp 
            580                 585                 590         


Phe Ile Ala Ser Ser Lys Arg Asp Gln Pro Ile Ile Leu Thr Ser Glu 
        595                 600                 605             


Leu Gly Lys Ser Tyr Asn Thr Ala Phe Glu Leu Asn Ile Asn Asp 
    610                 615                 620             


<210>  175
<211>  1626
<212>  DNA
<213>  Artificial

<220>
<223>  9G4-SGRS-hCXCL10 heavy chain

<400>  175
atgaacttcg gactctccct gatcttcctt gttctggtgc ttaagggcgt gcagtgcgaa       60

gtgatgttgg tagaaagcgg tggtgggctg gtgaaacctg gcgggtccct gaaactctcc      120

tgtactgcta gtggattcac attcagcact tacgacatgt catgggtccg gcagacccca      180

gagaagagac tggagtgggt cagcactatc tcctcttctc ggacctatac gtattacagt      240

gccagcgtca agggacgctt taccattagc agggataatg ccagaaacat actgtacctg      300

cagatgtcaa gcctccgaag tgaggacacc gccatgtatt actgcgctag gcaattttca      360

cactggggcc aggggacatt ggttaccgtg tctgcagcgt ccacaaaggg cccaagcgtg      420

ttcccgctag cccccagcag caagagcacc agcggcggca cagccgccct gggctgcctg      480

gtgaaggact acttccccga gcccgtgacc gtgtcctgga acagcggagc cctgacctcc      540

ggcgtgcaca ccttccccgc cgtgctgcag agcagcggcc tgtacagcct gagcagcgtg      600

gtgaccgtgc ccagcagcag cctgggcacc cagacctaca tctgtaacgt gaaccacaag      660

cccagcaaca ccaaggtgga caagagagtg gagcccaaga gctgtgacaa gacccacacc      720

tgtcccccct gcccagcccc cgagctgctg ggcggaccca gcgtgttcct gttccccccc      780

aagcccaagg acaccctgat gatcagcaga acccccgagg tgacctgtgt ggtggtggac      840

gtgtcccacg aggacccaga ggtgaagttc aactggtacg tggacggcgt ggaggtgcac      900

aacgccaaga ccaagcccag agaggagcag tacaacagca cctacagggt ggtgtccgtg      960

ctgaccgtgc tgcaccagga ctggctgaac ggcaaggagt acaagtgtaa ggtgtccaac     1020

aaggccctgc cagccccaat cgaaaagacc atcagcaagg ccaagggcca gccaagagag     1080

ccccaggtgt acaccctgcc acccagcagg gaggagatga ccaagaacca ggtgtccctg     1140

acctgtctgg tgaagggctt ctacccaagc gacatcgccg tggagtggga gagcaacggc     1200

cagcccgaga acaactacaa gaccaccccc ccagtgctgg acagcgacgg cagcttcttc     1260

ctgtacagca agctgaccgt ggacaagagc agatggcagc agggcaacgt gttcagctgc     1320

tccgtgatgc acgaggccct gcacaaccac tacacccaga agagcctgag cctgtcccca     1380

ggctcaggca gatctgtacc tctctctaga actgtacgct gtacctgcat cagcattagt     1440

aatcaacctg ttaatccaag gtctttagaa aaacttgaaa ttattcctgc aagccaattt     1500

tgtccacgtg ttgagatcat tgctacaatg aaaaagaagg gtgagaagag atgtctgaat     1560

ccagaatcga aggccatcaa gaatttactg aaagcagtta gcaaggaaag gtctaaaaga     1620

tctcct                                                                1626


<210>  176
<211>  542
<212>  PRT
<213>  Artificial

<220>
<223>  9G4-SGRS-hCXCL10 heavy chain

<400>  176

Met Asn Phe Gly Leu Ser Leu Ile Phe Leu Val Leu Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Met Leu Val Glu Ser Gly Gly Gly Leu Val Lys 
            20                  25                  30          


Pro Gly Gly Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Thr Tyr Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu 
    50                  55                  60                  


Glu Trp Val Ser Thr Ile Ser Ser Ser Arg Thr Tyr Thr Tyr Tyr Ser 
65                  70                  75                  80  


Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn 
                85                  90                  95      


Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gln Phe Ser His Trp Gly Gln Gly Thr Leu Val 
        115                 120                 125             


Thr Val Ser Ala Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
    130                 135                 140                 


Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
145                 150                 155                 160 


Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
                165                 170                 175     


Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
            180                 185                 190         


Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
        195                 200                 205             


Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
    210                 215                 220                 


Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr 
225                 230                 235                 240 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
                245                 250                 255     


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            260                 265                 270         


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        275                 280                 285             


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    290                 295                 300                 


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
305                 310                 315                 320 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                325                 330                 335     


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            340                 345                 350         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        355                 360                 365             


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    370                 375                 380                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
385                 390                 395                 400 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                405                 410                 415     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            420                 425                 430         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        435                 440                 445             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Ser Gly Arg 
    450                 455                 460                 


Ser Val Pro Leu Ser Arg Thr Val Arg Cys Thr Cys Ile Ser Ile Ser 
465                 470                 475                 480 


Asn Gln Pro Val Asn Pro Arg Ser Leu Glu Lys Leu Glu Ile Ile Pro 
                485                 490                 495     


Ala Ser Gln Phe Cys Pro Arg Val Glu Ile Ile Ala Thr Met Lys Lys 
            500                 505                 510         


Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu Ser Lys Ala Ile Lys Asn 
        515                 520                 525             


Leu Leu Lys Ala Val Ser Lys Glu Arg Ser Lys Arg Ser Pro 
    530                 535                 540         


<210>  177
<211>  1641
<212>  DNA
<213>  Artificial

<220>
<223>  NHS76-SGRS-hCXCL10 heavy chain

<400>  177
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc cggcccagga ctggtgaagc cttcggagac cctgtccctc      120

acctgcgctg tctctggtta ctccatcagc agtggttact actggggctg gattcggcag      180

cccccaggga aggggctgga gtggattggg agtatctatc atagtgggag cacctactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca cgtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcagac acggccgtgt attactgtgc aagagggaag      360

tggtcgaagt ttgactattg gggccaaggc accctggtca ccgtctcttc agcgtccaca      420

aagggcccaa gcgtgttccc gctagccccc agcagcaaga gcaccagcgg cggcacagcc      480

gccctgggct gcctggtgaa ggactacttc cccgagcccg tgaccgtgtc ctggaacagc      540

ggagccctga cctccggcgt gcacaccttc cccgccgtgc tgcagagcag cggcctgtac      600

agcctgagca gcgtggtgac cgtgcccagc agcagcctgg gcacccagac ctacatctgt      660

aacgtgaacc acaagcccag caacaccaag gtggacaaga gagtggagcc caagagctgt      720

gacaagaccc acacctgtcc cccctgccca gcccccgagc tgctgggcgg acccagcgtg      780

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gcagaacccc cgaggtgacc      840

tgtgtggtgg tggacgtgtc ccacgaggac ccagaggtga agttcaactg gtacgtggac      900

ggcgtggagg tgcacaacgc caagaccaag cccagagagg agcagtacaa cagcacctac      960

agggtggtgt ccgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag     1020

tgtaaggtgt ccaacaaggc cctgccagcc ccaatcgaaa agaccatcag caaggccaag     1080

ggccagccaa gagagcccca ggtgtacacc ctgccaccca gcagggagga gatgaccaag     1140

aaccaggtgt ccctgacctg tctggtgaag ggcttctacc caagcgacat cgccgtggag     1200

tgggagagca acggccagcc cgagaacaac tacaagacca cccccccagt gctggacagc     1260

gacggcagct tcttcctgta cagcaagctg accgtggaca agagcagatg gcagcagggc     1320

aacgtgttca gctgctccgt gatgcacgag gccctgcaca accactacac ccagaagagc     1380

ctgagcctgt ccccaggctc aggcagatct gtacctctct ctagaactgt acgctgtacc     1440

tgcatcagca ttagtaatca acctgttaat ccaaggtctt tagaaaaact tgaaattatt     1500

cctgcaagcc aattttgtcc acgtgttgag atcattgcta caatgaaaaa gaagggtgag     1560

aagagatgtc tgaatccaga atcgaaggcc atcaagaatt tactgaaagc agttagcaag     1620

gaaaggtcta aaagatctcc t                                               1641


<210>  178
<211>  547
<212>  PRT
<213>  Artificial

<220>
<223>  NHS76-SGRS-hCXCL10 heavy chain

<400>  178

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Tyr Ser 
        35                  40                  45              


Ile Ser Ser Gly Tyr Tyr Trp Gly Trp Ile Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Ser Ile Tyr His Ser Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Lys Trp Ser Lys Phe Asp Tyr Trp Gly 
        115                 120                 125             


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 
    130                 135                 140                 


Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 
145                 150                 155                 160 


Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 
                165                 170                 175     


Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 
            180                 185                 190         


Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
        195                 200                 205             


Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His 
    210                 215                 220                 


Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys 
225                 230                 235                 240 


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
                245                 250                 255     


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            260                 265                 270         


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        275                 280                 285             


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    290                 295                 300                 


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
305                 310                 315                 320 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                325                 330                 335     


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            340                 345                 350         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        355                 360                 365             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
385                 390                 395                 400 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                405                 410                 415     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            420                 425                 430         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        435                 440                 445             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    450                 455                 460                 


Pro Gly Ser Gly Arg Ser Val Pro Leu Ser Arg Thr Val Arg Cys Thr 
465                 470                 475                 480 


Cys Ile Ser Ile Ser Asn Gln Pro Val Asn Pro Arg Ser Leu Glu Lys 
                485                 490                 495     


Leu Glu Ile Ile Pro Ala Ser Gln Phe Cys Pro Arg Val Glu Ile Ile 
            500                 505                 510         


Ala Thr Met Lys Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro Glu Ser 
        515                 520                 525             


Lys Ala Ile Lys Asn Leu Leu Lys Ala Val Ser Lys Glu Arg Ser Lys 
    530                 535                 540                 


Arg Ser Pro 
545         


<210>  179
<211>  1647
<212>  DNA
<213>  Artificial

<220>
<223>  H16L16-SGRS-hCXCL10 heavy chain

<400>  179
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc      120

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      180

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      360

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      420

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      480

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      540

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      600

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      660

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      720

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      780

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      840

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      900

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      960

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag     1020

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1080

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1140

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1200

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1260

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1320

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1380

aagagcctga gcctgtcccc aggctcaggc agatctgtac ctctctctag aactgtacgc     1440

tgtacctgca tcagcattag taatcaacct gttaatccaa ggtctttaga aaaacttgaa     1500

attattcctg caagccaatt ttgtccacgt gttgagatca ttgctacaat gaaaaagaag     1560

ggtgagaaga gatgtctgaa tccagaatcg aaggccatca agaatttact gaaagcagtt     1620

agcaaggaaa ggtctaaaag atctcct                                         1647


<210>  180
<211>  549
<212>  PRT
<213>  Artificial

<220>
<223>  H16L16-SGRS-hCXCL10 heavy chain

<400>  180

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gly Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser 
        35                  40                  45              


Ile Ser Ser Ser Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Ser Gly Arg Ser Val Pro Leu Ser Arg Thr Val Arg 
465                 470                 475                 480 


Cys Thr Cys Ile Ser Ile Ser Asn Gln Pro Val Asn Pro Arg Ser Leu 
                485                 490                 495     


Glu Lys Leu Glu Ile Ile Pro Ala Ser Gln Phe Cys Pro Arg Val Glu 
            500                 505                 510         


Ile Ile Ala Thr Met Lys Lys Lys Gly Glu Lys Arg Cys Leu Asn Pro 
        515                 520                 525             


Glu Ser Lys Ala Ile Lys Asn Leu Leu Lys Ala Val Ser Lys Glu Arg 
    530                 535                 540                 


Ser Lys Arg Ser Pro 
545                 


<210>  181
<211>  1761
<212>  DNA
<213>  Artificial

<220>
<223>  H16L16-SGRSA-hIL15 heavy chain

<400>  181
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc      120

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      180

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      360

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      420

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      480

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      540

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      600

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      660

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      720

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      780

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      840

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      900

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      960

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag     1020

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1080

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1140

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1200

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1260

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1320

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1380

aagagcctga gcctgtcccc aggctcaggc agatctgcca actgggtgaa tgtaataagt     1440

gatttgaaaa aaattgaaga tcttattcaa tctatgcata ttgatgctac tttatatacg     1500

gaaagtgatg ttcaccccag ttgcaaagta acagcaatga agtgctttct cttggagtta     1560

caagttattt cacttgagtc cggagatgca agtattcatg atacagtaga aaatctgatc     1620

atcctagcaa acaacagttt gtcttctaat gggaatgtaa cagaatctgg atgcaaagaa     1680

tgtgaggaac tggaggaaaa aaatattaaa gaatttttgc agagttttgt acatattgtc     1740

caaatgttca tcaacacttc t                                               1761


<210>  182
<211>  587
<212>  PRT
<213>  Artificial

<220>
<223>  H16L16-SGRSA-hIL15 heavy chain

<400>  182

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gly Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser 
        35                  40                  45              


Ile Ser Ser Ser Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Ser Gly Arg Ser Ala Asn Trp Val Asn Val Ile Ser 
465                 470                 475                 480 


Asp Leu Lys Lys Ile Glu Asp Leu Ile Gln Ser Met His Ile Asp Ala 
                485                 490                 495     


Thr Leu Tyr Thr Glu Ser Asp Val His Pro Ser Cys Lys Val Thr Ala 
            500                 505                 510         


Met Lys Cys Phe Leu Leu Glu Leu Gln Val Ile Ser Leu Glu Ser Gly 
        515                 520                 525             


Asp Ala Ser Ile His Asp Thr Val Glu Asn Leu Ile Ile Leu Ala Asn 
    530                 535                 540                 


Asn Ser Leu Ser Ser Asn Gly Asn Val Thr Glu Ser Gly Cys Lys Glu 
545                 550                 555                 560 


Cys Glu Glu Leu Glu Glu Lys Asn Ile Lys Glu Phe Leu Gln Ser Phe 
                565                 570                 575     


Val His Ile Val Gln Met Phe Ile Asn Thr Ser 
            580                 585         


<210>  183
<211>  1914
<212>  DNA
<213>  Artificial

<220>
<223>  H16L16-SGRSA-hIFNa heavy chain

<400>  183
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc      120

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      180

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      360

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      420

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      480

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      540

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      600

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      660

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      720

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      780

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      840

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      900

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      960

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag     1020

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1080

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1140

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1200

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1260

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1320

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1380

aagagcctga gcctgtcccc aggctcaggc agatctgcct gtgatctgcc tcaaacccac     1440

agcctgggta gcaggaggac cttgatgctc ctggcacaga tgaggaaaat ctctcttttc     1500

tcctgcttga aggacagaca tgactttgga tttccccagg aggagtttgg caaccagttc     1560

caaaaggctg aaaccatccc tgtcctccat gagatgatcc agcagatctt caatctcttc     1620

agcacaaagg actcatctgc tgcttgggat gagaccctcc tagacaaatt ctacactgaa     1680

ctctaccagc agctgaatga cctggaagcc tgtgtgatac agggggtggg ggtgacagag     1740

actcccctga tgaaggagga ctccattctg gctgtgagga aatacttcca aagaatcact     1800

ctctatctga aagagaagaa atacagccct tgtgcctggg aggttgtcag agcagaaatc     1860

atgagatctt tttctttgtc aacaaacttg caagaaagtt taagaagtaa ggaa           1914


<210>  184
<211>  638
<212>  PRT
<213>  Artificial

<220>
<223>  H16L16-SGRSA-hIFNa heavy chain

<400>  184

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gly Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser 
        35                  40                  45              


Ile Ser Ser Ser Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Ser Gly Arg Ser Ala Cys Asp Leu Pro Gln Thr His 
465                 470                 475                 480 


Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Lys 
                485                 490                 495     


Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro 
            500                 505                 510         


Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val 
        515                 520                 525             


Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp 
    530                 535                 540                 


Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu 
545                 550                 555                 560 


Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val 
                565                 570                 575     


Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val 
            580                 585                 590         


Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr 
        595                 600                 605             


Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe 
    610                 615                 620                 


Ser Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys Glu 
625                 630                 635             


<210>  185
<211>  1923
<212>  DNA
<213>  Artificial

<220>
<223>  H16L16-PSSRRRVN-hIFNa heavy chain

<400>  185
atggaaaccg acaccctgct gctgtgggtg ctgctgctct gggtcccagg ctctaccggc       60

caggtgcagc tgcaggagtc gggaccagga ctggtgaagc cttcggggac cctgtccctc      120

acctgcgctg tctctggtgg ctccatcagc agtagtaact ggtggagttg ggtccgccag      180

cccccaggga aggggctgga gtggattggg gaaatctatc atagtgggag caccaactac      240

aacccgtccc tcaagagtcg agtcaccata tcagtagaca agtccaagaa ccagttctcc      300

ctgaagctga gctctgtgac cgccgcggac acggccgtgt attactgtgc gagatggacc      360

gggcgtactg atgcttttga tatctggggc caagggacaa tggtcaccgt ctcaagcgcg      420

tccacaaagg gcccaagcgt gttcccgcta gcccccagca gcaagagcac cagcggcggc      480

acagccgccc tgggctgcct ggtgaaggac tacttccccg agcccgtgac cgtgtcctgg      540

aacagcggag ccctgacctc cggcgtgcac accttccccg ccgtgctgca gagcagcggc      600

ctgtacagcc tgagcagcgt ggtgaccgtg cccagcagca gcctgggcac ccagacctac      660

atctgtaacg tgaaccacaa gcccagcaac accaaggtgg acaagagagt ggagcccaag      720

agctgtgaca agacccacac ctgtcccccc tgcccagccc ccgagctgct gggcggaccc      780

agcgtgttcc tgttcccccc caagcccaag gacaccctga tgatcagcag aacccccgag      840

gtgacctgtg tggtggtgga cgtgtcccac gaggacccag aggtgaagtt caactggtac      900

gtggacggcg tggaggtgca caacgccaag accaagccca gagaggagca gtacaacagc      960

acctacaggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaggag     1020

tacaagtgta aggtgtccaa caaggccctg ccagccccaa tcgaaaagac catcagcaag     1080

gccaagggcc agccaagaga gccccaggtg tacaccctgc cacccagcag ggaggagatg     1140

accaagaacc aggtgtccct gacctgtctg gtgaagggct tctacccaag cgacatcgcc     1200

gtggagtggg agagcaacgg ccagcccgag aacaactaca agaccacccc cccagtgctg     1260

gacagcgacg gcagcttctt cctgtacagc aagctgaccg tggacaagag cagatggcag     1320

cagggcaacg tgttcagctg ctccgtgatg cacgaggccc tgcacaacca ctacacccag     1380

aagagcctga gcctgtcccc aggcccgagc agccggagaa gagtgaactg tgatctgcct     1440

caaacccaca gcctgggtag caggaggacc ttgatgctcc tggcacagat gaggaaaatc     1500

tctcttttct cctgcttgaa ggacagacat gactttggat ttccccagga ggagtttggc     1560

aaccagttcc aaaaggctga aaccatccct gtcctccatg agatgatcca gcagatcttc     1620

aatctcttca gcacaaagga ctcatctgct gcttgggatg agaccctcct agacaaattc     1680

tacactgaac tctaccagca gctgaatgac ctggaagcct gtgtgataca gggggtgggg     1740

gtgacagaga ctcccctgat gaaggaggac tccattctgg ctgtgaggaa atacttccaa     1800

agaatcactc tctatctgaa agagaagaaa tacagccctt gtgcctggga ggttgtcaga     1860

gcagaaatca tgagatcttt ttctttgtca acaaacttgc aagaaagttt aagaagtaag     1920

gaa                                                                   1923


<210>  186
<211>  641
<212>  PRT
<213>  Artificial

<220>
<223>  H16L16-PSSRRRVN-hIFNa heavy chain

<400>  186

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gly Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Gly Ser 
        35                  40                  45              


Ile Ser Ser Ser Asn Trp Trp Ser Trp Val Arg Gln Pro Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Glu Ile Tyr His Ser Gly Ser Thr Asn Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Val Thr Ile Ser Val Asp Lys Ser Lys 
                85                  90                  95      


Asn Gln Phe Ser Leu Lys Leu Ser Ser Val Thr Ala Ala Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Trp Thr Gly Arg Thr Asp Ala Phe Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Ser Thr Lys Gly 
    130                 135                 140                 


Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
145                 150                 155                 160 


Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val 
                165                 170                 175     


Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe 
            180                 185                 190         


Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val 
        195                 200                 205             


Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val 
    210                 215                 220                 


Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys 
225                 230                 235                 240 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
305                 310                 315                 320 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
            340                 345                 350         


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            420                 425                 430         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 


Leu Ser Pro Gly Pro Ser Ser Arg Arg Arg Val Asn Cys Asp Leu Pro 
465                 470                 475                 480 


Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met Leu Leu Ala Gln 
                485                 490                 495     


Met Arg Lys Ile Ser Leu Phe Ser Cys Leu Lys Asp Arg His Asp Phe 
            500                 505                 510         


Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr 
        515                 520                 525             


Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe Asn Leu Phe Ser 
    530                 535                 540                 


Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe 
545                 550                 555                 560 


Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu Ala Cys Val Ile 
                565                 570                 575     


Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys Glu Asp Ser Ile 
            580                 585                 590         


Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu Tyr Leu Lys Glu 
        595                 600                 605             


Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg Ala Glu Ile Met 
    610                 615                 620                 


Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys 
625                 630                 635                 640 


Glu 
    


