               SEQUENCE LISTING

<110> ORTA DOĞU TEKNİK ÜNİVERSİTESİ

<120> DESIGN OF ALCOHOL DEHYDROGENASE 2 (ADH2) PROMOTER VARIANTS BY PROMOTER ENGINEERING

<130> P19-0923

<140> PCT/TR2019/050784
<141> 2019-09-23

<150> TR2018/13725
<151> 2018-09-24

<160> 2

<170> BiSSAP 1.3.6

<210> 1
<211> 1047
<212> DNA
<213> Pichia pastoris


<220> 
<223> ADH2 wild type promoter

<400> 1
tcctttttac cacccaagtg cgagtgaaac accccatggc tgctctccga ttgcccctct      60

acaggcataa gggtgtgact ttgtgggctt gaattttaca ccccctccaa cttttctcgc     120

atcaattgat cctgttacca atattgcatg cccggaggag acttgccccc taatttcgcg     180

gcgtcgtccc ggatcgcagg gtgagactgt agagacccca catagtgaca atgattatgt     240

aagaagaggg gggtgattcg gccggctatc gaactctaac aactaggggg gtgaacaatg     300

cccagcagtc ctccccactc tttgacaaat cagtatcacc gattaacacc ccaaatctta     360

ttctcaacgg tccctcatcc ttgcacccct ctttggacaa atggcagtta gcattggtgc     420

actgactgac tgcccaacct taaacccaaa tttcttagaa ggggcccatc tagttagcga     480

ggggtgaaaa attcctccat cggagatgta ttgaccgtaa gttgctgctt aaaaaaaatc     540

agttcagata gcgagacttt tttgatttcg caacgggagt gcctgttcca ttcgattgca     600

attctcaccc cttctgccca gtcctgccaa ttgcccatga atctgctaat ttcgttgatt     660

cccacccccc tttccaactc cacaaattgt ccaatctcgt tttccatttg ggagaatctg     720

catgtcgact acataaagcg accggtgtcc gaaaagatct gtgtagtttt caacattttg     780

tgctcccccc gctgtttgaa aacgggggtg agcgctctcc ggggtgcgaa ttcgtgccca     840

attcctttca ccctgcctat tgtagacgtc aacccgcatc tggtgcgaat atagcgcacc     900

cccaatgatc acaccaacaa ttggtccacc cctccccaat ctctaatatt cacaattcac     960

ctcactataa atacccctgt cctgctccca aattcttttt tccttcttcc atcagctact    1020

agcttttatc ttatttactt tacgaaa                                        1047


<210> 2
<211> 1047
<212> DNA
<213> Artificial Sequence


<220> 
<223> Modified nucleosome optimized ADH2 promoter variant

<400> 2
tcctttttac cacctaagtg cgagtgaaac accctatggc tgctctccga ttgcccctct      60

acaggcataa gggtgtgatt tttttttttt taattttaca ccccctccaa cttttttcgc     120

gtaaattgat cctgttacca atattgcatg cccggaggag acttgccccc taatttcgcg     180

gcgtcgtccc ggatcgcagg gtaaaaatat atagacccca caaaaaaaaa atgattatgt     240

aagaagaggg gggtgattcg gccggctatc gaactctaac aactaggggg gtgaaaaatg     300

cccagctttt ttccctattc tttgacaaat cagtatcact tattaacacc ccaaattttt     360

ttctcaacgg tccctcatcc ttgcacccct ctttggacaa atggcagtta gtattagtgc     420

actgactgac tgcctaacct taaaccctaa tttcttagaa ggggcccata tagttagcga     480

ggggtgaaaa attcctccat cggagatgta ttaaccgtaa tttttttttt aaaaaaaaaa     540

aattcagata gcgaaatttt tttgatttcg cgacgcgcgt tttttttttt tttttttttt     600

tttctcaccc cttctgccca gttctgccaa ttgcccatga atctactaat ttcgttgatt     660

cccacccccc tttccaactc caaaaatttt ttaatttttt tttttttttg ggagaatctg     720

aatgtatatt acataaagcg accggtgtcc gaaaaaattt tttttttttt taattttttt     780

tttttccccc gctttttaaa aacgggggta agcgctctcc ggggtgcgaa ttcgcgccct     840

attcctttca ccctgcctat tgtagacgtc aacccgcatc tggtgcgaat atagcgcacc     900

cccaatgatc acaccaacaa ttggtccacc cctccccaat ctctaatatt cacaattcac     960

ctcactataa atacccctgt cctgctccca aattcttttt tccttcttcc atcagctact    1020

agcttttatc ttatttactt tacgaaa                                        1047


