                         SEQUENCE LISTING

<110>  ALBERT EINSTEIN COLLEGE OF MEDICINE
 
<120>  MUTANT VARIANTS OF PD-1 RECEPTOR WITH SELECTIVE BINDING TO PD-L1 
       AND USES THEREOF

<130>  96700/2677

<150>  US 62/736,477
<151>  2018-09-26

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  288
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  2
<211>  117
<212>  PRT
<213>  Homo sapiens

<400>  2

Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
            20                  25                  30          


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
        35                  40                  45              


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
    50                  55                  60                  


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
65                  70                  75                  80  


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
            100                 105                 110         


Thr Glu Arg Arg Ala 
        115         


<210>  3
<211>  114
<212>  PRT
<213>  Mus musculus

<400>  3

Ser Leu Thr Phe Tyr Pro Ala Thr Arg Leu Thr Val Ser Glu Gly Ala 
1               5                   10                  15      


Asn Ala Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met 
            20                  25                  30          


Leu Asn Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala 
        35                  40                  45              


Ala Phe Ser Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln 
    50                  55                  60                  


Ile Ile Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp 
65                  70                  75                  80  


Thr Arg Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu 
                85                  90                  95      


His Pro Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val 
            100                 105                 110         


Thr Glu 
        


<210>  4
<211>  101
<212>  PRT
<213>  Homo sapiens

<400>  4

Leu Phe Thr Val Thr Val Pro Lys Glu Leu Tyr Ile Ile Glu His Gly 
1               5                   10                  15      


Ser Asn Val Thr Leu Glu Cys Asn Phe Asp Thr Gly Ser His Val Asn 
            20                  25                  30          


Leu Gly Ala Ile Thr Ala Ser Leu Gln Lys Val Glu Asn Asp Thr Ser 
        35                  40                  45              


Pro His Arg Glu Arg Ala Thr Leu Leu Glu Glu Gln Leu Pro Leu Gly 
    50                  55                  60                  


Lys Ala Ser Phe His Ile Pro Gln Val Gln Val Arg Asp Glu Gly Gln 
65                  70                  75                  80  


Tyr Gln Cys Ile Ile Ile Tyr Gly Val Ala Trp Asp Tyr Lys Tyr Leu 
                85                  90                  95      


Thr Leu Lys Val Lys 
            100     


<210>  5
<211>  102
<212>  PRT
<213>  Mus musculus

<400>  5

Leu Phe Thr Val Thr Ala Pro Lys Glu Val Tyr Thr Val Asp Val Gly 
1               5                   10                  15      


Ser Ser Val Ser Leu Glu Cys Asp Phe Asp Arg Arg Glu Cys Thr Glu 
            20                  25                  30          


Leu Glu Gly Ile Ile Arg Ala Ser Leu Gln Lys Val Glu Asn Asp Thr 
        35                  40                  45              


Ser Leu Gln Ser Glu Arg Ala Thr Leu Leu Glu Glu Gln Leu Pro Leu 
    50                  55                  60                  


Gly Lys Ala Leu Phe His Ile Pro Ser Val Gln Val Arg Asp Ser Gly 
65                  70                  75                  80  


Gln Tyr Arg Cys Leu Val Ile Cys Gly Ala Ala Trp Asp Tyr Lys Tyr 
                85                  90                  95      


Leu Thr Val Lys Val Lys 
            100         


<210>  6
<211>  114
<212>  PRT
<213>  Homo sapiens

<400>  6

Ala Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly 
1               5                   10                  15      


Ser Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu Asp 
            20                  25                  30          


Leu Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile 
        35                  40                  45              


Gln Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser Tyr 
    50                  55                  60                  


Arg Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn Ala 
65                  70                  75                  80  


Ala Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg 
                85                  90                  95      


Cys Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val Lys 
            100                 105                 110         


Val Asn 
        


<210>  7
<211>  101
<212>  PRT
<213>  Mus musculus

<400>  7

Leu Phe Thr Val Thr Val Pro Lys Glu Leu Tyr Ile Ile Glu His Gly 
1               5                   10                  15      


Ser Asn Val Thr Leu Glu Cys Asn Phe Asp Thr Gly Ser His Val Asn 
            20                  25                  30          


Leu Gly Ala Ile Thr Ala Ser Leu Gln Lys Val Glu Asn Asp Thr Ser 
        35                  40                  45              


Pro His Arg Glu Arg Ala Thr Leu Leu Glu Glu Gln Leu Pro Leu Gly 
    50                  55                  60                  


Lys Ala Ser Phe His Ile Pro Gln Val Gln Val Arg Asp Glu Gly Gln 
65                  70                  75                  80  


Tyr Gln Cys Ile Ile Ile Tyr Gly Val Ala Trp Asp Tyr Lys Tyr Leu 
                85                  90                  95      


Thr Leu Lys Val Lys 
            100     


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  8

Asp Glu Pro Asn Gln Arg His Thr 
1               5               


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  9

His Lys Asn Gln Arg Thr Trp 
1               5           


<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  10

His Lys Asn Gln Trp 
1               5   


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  11

His Lys Asn Gln Arg Trp Tyr 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  12

His Lys Asn Gln Arg Trp Tyr 
1               5           


<210>  13
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  13

His Ile Lys Leu Met Asn Pro Gln Arg Val Trp Tyr 
1               5                   10          


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  14

Gln Glu Asp Thr Asn Phe 
1               5       


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  15

His Asn Pro Gln Arg Ser Thr Trp Tyr 
1               5                   


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  16

His Lys Asn Pro Gln Arg Thr Trp Tyr 
1               5                   


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  17

Lys Asn Gln Arg Thr Trp Tyr 
1               5           


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  18

Asp Glu Pro Gln Ser Tyr 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  19

Phe Ile Met Val Trp Tyr 
1               5       


<210>  20
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  20

Asp Phe His Ile Lys Leu Met Asn Pro Gln Arg Ser Thr Val Trp Tyr 
1               5                   10                  15      


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  21

Lys Asn Gln Arg Ser Thr Trp Tyr 
1               5               


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  22

His Lys Asn Gln Arg Thr Trp Tyr 
1               5               


<210>  23
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  23

Lys Arg Asn Gln 
1               


<210>  24
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  24

His Lys Asn Gln Arg Ser Trp Tyr 
1               5               


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  25

Asp Glu Phe His Ile Lys Leu Met 
1               5               


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  26

Asn Pro Gln Arg Thr Val Trp Tyr 
1               5               


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  27

Asp Gln Glu Asn Ser Tyr 
1               5       


<210>  28
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  28

Asp Glu Phe His Leu Met Asn Pro 
1               5               


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  29

Gln Arg Ser Thr Val Trp Tyr 
1               5           


<210>  30
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  30

Asp Glu Asn Pro Gln Ser Thr Tyr 
1               5               


<210>  31
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  31

Asp Glu Asn Gln Ser Thr 
1               5       


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  32

His Asn Pro Gln Ser Thr Trp 
1               5           


<210>  33
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rs-pharmacophore

<400>  33

His Ile Lys Leu Met Asn Pro Gln Arg Ser Thr Val Trp Tyr 
1               5                   10                  


