                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
 
<120> COMPOSITIONS AND METHODS COMPRISING MUTANTS OF TERMINAL 
      DEOXYNUCLEOTIDYL TRANSFERASE

<130> 002806-093400WOPT

<140> PCT/US2019/054398
<141> 2019-10-03

<150> 62/741,143
<151> 2018-10-04

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 509
<212> PRT
<213> Homo sapiens

<400> 1
Met Asp Pro Pro Arg Ala Ser His Leu Ser Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Thr Gly Ala Leu Met Ala Ser Ser Pro Gln Asp Ile Lys Phe 
            20                  25                  30          


Gln Asp Leu Val Val Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg 
        35                  40                  45              


Arg Ala Phe Leu Met Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Asp Val Leu Glu Trp Leu Gln Ala Gln Lys Val Gln Val Ser 
                85                  90                  95      


Ser Gln Pro Glu Leu Leu Asp Val Ser Trp Leu Ile Glu Cys Ile Arg 
            100                 105                 110         


Ala Gly Lys Pro Val Glu Met Thr Gly Lys His Gln Leu Val Val Arg 
        115                 120                 125             


Arg Asp Tyr Ser Asp Ser Thr Asn Pro Gly Pro Pro Lys Thr Pro Pro 
    130                 135                 140                 


Ile Ala Val Gln Lys Ile Ser Gln Tyr Ala Cys Gln Arg Arg Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Cys Asn Gln Ile Phe Thr Asp Ala Phe Asp Ile Leu Ala 
                165                 170                 175     


Glu Asn Cys Glu Phe Arg Glu Asn Glu Asp Ser Cys Val Thr Phe Met 
            180                 185                 190         


Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met 
        195                 200                 205             


Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Ser Lys Val Lys Gly Ile 
    210                 215                 220                 


Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Thr Leu Ser Lys Val Arg Ser Asp Lys Ser Leu Lys Phe Thr Arg Met 
        275                 280                 285             


Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr 
    290                 295                 300                 


Arg Ala Glu Ala Glu Ala Val Ser Val Leu Val Lys Glu Ala Val Trp 
305                 310                 315                 320 


Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Lys Met Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly 
            340                 345                 350         


Ser Thr Glu Asp Glu Glu Gln Leu Leu Gln Lys Val Met Asn Leu Trp 
        355                 360                 365             


Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe 
    370                 375                 380                 


Glu Lys Leu Arg Leu Pro Ser Arg Lys Val Asp Ala Leu Asp His Phe 
385                 390                 395                 400 


Gln Lys Cys Phe Leu Ile Phe Lys Leu Pro Arg Gln Arg Val Asp Ser 
                405                 410                 415     


Asp Gln Ser Ser Trp Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Asp 
            420                 425                 430         


Asp Leu Val Leu Cys Pro Tyr Glu Arg Arg Ala Phe Ala Leu Leu Gly 
        435                 440                 445             


Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr Ala Thr 
    450                 455                 460                 


His Glu Arg Lys Met Ile Leu Asp Asn His Ala Leu Tyr Asp Lys Thr 
465                 470                 475                 480 


Lys Arg Ile Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His 
                485                 490                 495     


Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 505                 


<210> 2
<211> 510
<212> PRT
<213> Ambystoma mexicanum

<400> 2
Met Tyr Ala Phe Pro Thr Thr Arg Ile Ala Pro Arg Arg Lys Gln Pro 
1               5                   10                  15      


Lys Cys Ile Lys Pro Pro Lys Cys Thr Ser Lys Tyr Asp Ile Lys Phe 
            20                  25                  30          


Lys Asp Ile Ala Ile Tyr Ile Leu Glu Arg Lys Met Gly Ala Ser Arg 
        35                  40                  45              


Arg Tyr Phe Leu Met Glu Leu Ala Arg Lys Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Pro Asp Leu Ser Glu Tyr Val Thr His Val Val Ser Glu Lys Asn Ser 
65                  70                  75                  80  


Gly Ala Glu Val Leu Glu Trp Leu Gln Ala Lys Lys Ala Gly Ser Ile 
                85                  90                  95      


Pro Asn Val Ala Ile Leu Asp Ile Ser Trp Phe Thr Asp Cys Met Gly 
            100                 105                 110         


Ala Gly Gln Pro Val Glu Ile Glu Arg Lys His Arg Leu Thr Leu Gln 
        115                 120                 125             


Lys Ile Cys Val Cys Lys Ser Pro Ser Pro Val Val Pro Ser Arg Val 
    130                 135                 140                 


Gly Val Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr Leu Asp Asn Lys 
145                 150                 155                 160 


Asn Thr Leu Phe Thr Asp Ala Phe Glu Ile Leu Ala Glu Asn Tyr Glu 
                165                 170                 175     


Phe Arg Glu Asn Glu Arg Ser Cys Leu Ser Phe Arg Gln Ala Ala Ser 
            180                 185                 190         


Val Leu Lys Ser Leu Thr Phe Thr Ile Ala Gly Met Ala Asp Val Asp 
        195                 200                 205             


Gly Leu Pro Gly Phe Gly Asp His Ile Arg Ala Val Ile Glu Asp Leu 
    210                 215                 220                 


Ile Glu Asp Gly Glu Ser Ser Lys Val Ser Glu Val Leu Asn Asp Glu 
225                 230                 235                 240 


Val Tyr Arg Ser Leu Lys Leu Phe Thr Thr Ile Phe Gly Val Gly Leu 
                245                 250                 255     


Arg Thr Ala Glu Lys Trp His Arg Leu Gly Ile Arg Thr Leu Glu Glu 
            260                 265                 270         


Ile Lys Ser Asn Glu Asn Leu Lys Phe Ser Lys Met Gln Ile Ala Gly 
        275                 280                 285             


Leu Gln His Tyr Glu Asp Ile Leu Gly Gly Val Arg Lys Ala Glu Ala 
    290                 295                 300                 


Asp Ala Val Ala Met Val Val Arg Asp Ala Val Trp Thr Phe Leu Pro 
305                 310                 315                 320 


Asp Ala Val Val Thr Leu Thr Gly Gly Phe Arg Arg Gly Asn Lys Thr 
                325                 330                 335     


Gly His Asp Val Asp Met Leu Ile Thr Ser Pro Ile Gln Gly Lys Glu 
            340                 345                 350         


Lys Glu Leu Leu His Lys Val Ile Asn Leu Trp Lys Lys Gln Asp Leu 
        355                 360                 365             


Leu Leu Cys His Thr Ile His Glu Ser Thr Met Asp Glu Asp Asn Leu 
    370                 375                 380                 


Pro Ser Lys Ser Val Asn Leu Leu Asp His Phe Gln Lys Cys Phe Ala 
385                 390                 395                 400 


Ile Leu Lys Ser Asn Gln His Arg Gly Glu Ile Ser Ser Cys Asp Gly 
                405                 410                 415     


Pro His Asp Ser Arg Glu Arg Gly Lys Arg Ile Trp Lys Ala Ile Arg 
            420                 425                 430         


Val Asp Leu Val Phe Cys Pro Phe Glu Gln Tyr Ala Phe Ala Leu Leu 
        435                 440                 445             


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr Ala 
    450                 455                 460                 


Ser His Glu Lys Lys Met Met Ile Asp Asn His Ala Leu Tyr Asp Lys 
465                 470                 475                 480 


Thr Lys Arg Val Phe Val Lys Cys Glu Ser Glu Glu Glu Ile Phe Gly 
                485                 490                 495     


His Leu Gly Leu Glu Tyr Ile Asp Pro Val Glu Arg Asn Ala 
            500                 505                 510 


<210> 3
<211> 511
<212> PRT
<213> Eulemur macaco

<400> 3
Met Asp Pro Leu Gln Thr Ala His Ala Gly Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Thr Gly Ala Ser Met Ala Ser Thr Pro Gln Asp Val Arg Phe 
            20                  25                  30          


Gln Asp Leu Val Leu Phe Ile Leu Glu Lys Lys Met Gly Ser Thr Arg 
        35                  40                  45              


Arg Thr Phe Leu Thr Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Phe Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Asp Val Leu Glu Trp Leu Gln Val Gln Lys Ile Lys Ala Ser 
                85                  90                  95      


Ser Gln Leu Glu Val Leu Asp Val Ser Trp Leu Ile Glu Cys Met Arg 
            100                 105                 110         


Thr Gly Lys Pro Val Glu Thr Thr Gly Lys His Gln Leu Val Ala Arg 
        115                 120                 125             


Ser Asp Cys Ser Ala Ser Pro Asn Pro Gly Pro Gln Lys Thr Pro Pro 
    130                 135                 140                 


Leu Ala Val Gln Lys Ile Ser Gln Tyr Ala Cys Gln Arg Arg Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Cys Asn His Val Phe Thr Asp Ala Phe Glu Ile Leu Ala 
                165                 170                 175     


Glu Asn Tyr Glu Phe Lys Glu Asn Glu Gly Cys Ala Val Thr Phe Leu 
            180                 185                 190         


Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Thr Met 
        195                 200                 205             


Arg Asp Thr Glu Gly Ile Pro Cys Leu Glu Asp Lys Val Lys Cys Ile 
    210                 215                 220                 


Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Thr Leu Ser Lys Ile Arg Ser Asp Lys Ser Leu Thr Phe Thr Arg Met 
        275                 280                 285             


Gln Arg Ala Gly Phe His Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr 
    290                 295                 300                 


Arg Ala Glu Ala Glu Ala Val Ser Val Leu Val Lys Glu Ala Val Trp 
305                 310                 315                 320 


Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly 
            340                 345                 350         


Ser Thr Glu Glu Glu Glu Glu Gln Leu Leu His Lys Val Met Asn Leu 
        355                 360                 365             


Trp Glu Lys Lys Gly Leu Leu Leu Tyr Cys Asp Leu Val Glu Ser Thr 
    370                 375                 380                 


Phe Glu Lys Leu Lys Leu Pro Ser Arg Lys Val Asp Ala Leu Asp His 
385                 390                 395                 400 


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Val 
                405                 410                 415     


Asp Ser Gln Lys Ser Ser Gln Gln Asp Gly Lys Thr Trp Lys Ala Ile 
            420                 425                 430         


Arg Val Asp Leu Val Met Cys Pro Tyr Glu Arg Arg Ala Phe Ala Leu 
        435                 440                 445             


Leu Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr 
    450                 455                 460                 


Ala Thr His Glu Arg Arg Met Met Leu Asp Asn His Gly Leu Trp Asp 
465                 470                 475                 480 


Lys Thr Lys Arg Ile Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe 
                485                 490                 495     


Ala His Leu Gly Leu Asp Tyr Ile Glu Pro Ser Glu Arg Asn Ala 
            500                 505                 510     


<210> 4
<211> 518
<212> PRT
<213> Monodelphis domestica

<400> 4
Met His Arg Ile Arg Thr Ile Asp Ser Asp Phe Gly Lys Lys Arg Gln 
1               5                   10                  15      


Lys Lys Met Asp Asn His Ile Ser Ser Met Ile Tyr Glu Ile Lys Phe 
            20                  25                  30          


His Glu Phe Val Leu Phe Ile Leu Glu Lys Lys Met Gly Ala Thr Arg 
        35                  40                  45              


Arg Thr Phe Leu Thr Asp Leu Ala Arg Lys Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asn Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Asp Val Leu Ala Trp Leu Lys Thr His Lys Met Glu Lys Thr 
                85                  90                  95      


Thr Gln Phe Glu Leu Leu Asp Ile Ser Trp Leu Ile Glu Cys Met Lys 
            100                 105                 110         


Val Gly Lys Pro Val Asp Thr Lys Gly Lys Tyr Gln Leu Met Glu Ser 
        115                 120                 125             


Arg Val Asp Ser Ala Asn Pro Asp Pro Thr Ala Gly Thr Leu Asn Ile 
    130                 135                 140                 


Leu Pro Pro Thr Thr Lys Thr Ile Ser Gln Tyr Ala Cys Gln Arg Arg 
145                 150                 155                 160 


Thr Thr Ile Asn Asn His Asn Gln Arg Phe Thr Asp Ala Phe Glu Ile 
                165                 170                 175     


Leu Ala Lys Asn Tyr Glu Phe Lys Glu Asn Asp Asp Thr Cys Leu Thr 
            180                 185                 190         


Phe Met Arg Ala Ile Ser Val Leu Lys Cys Leu Pro Phe Glu Val Val 
        195                 200                 205             


Ser Leu Lys Asp Thr Glu Gly Leu Pro Trp Ile Gly Asp Glu Val Lys 
    210                 215                 220                 


Gly Ile Met Glu Glu Ile Ile Glu Asp Gly Glu Ser Leu Glu Val Gln 
225                 230                 235                 240 


Ala Val Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser 
                245                 250                 255     


Val Phe Gly Val Gly Leu Lys Thr Ala Asp Lys Trp Tyr Arg Met Gly 
            260                 265                 270         


Phe Arg Thr Leu Asn Lys Ile Arg Ser Asp Lys Thr Leu Lys Leu Thr 
        275                 280                 285             


Lys Met Gln Lys Ala Gly Leu Cys Tyr Tyr Glu Asp Leu Ile Asp Cys 
    290                 295                 300                 


Val Ser Lys Ala Glu Ala Asp Ala Val Ser Leu Leu Val Gln Asp Ala 
305                 310                 315                 320 


Val Trp Thr Phe Leu Pro Asp Ala Leu Val Thr Ile Thr Gly Gly Phe 
                325                 330                 335     


Arg Arg Gly Lys Glu Phe Gly His Asp Val Asp Phe Leu Ile Thr Ser 
            340                 345                 350         


Pro Gly Ala Glu Lys Glu Gln Glu Asp Gln Leu Leu Gln Lys Val Thr 
        355                 360                 365             


Asn Leu Trp Lys Lys Gln Gly Leu Leu Leu Tyr Cys Asp Leu Ile Glu 
    370                 375                 380                 


Ser Thr Phe Glu Asp Leu Lys Leu Pro Ser Arg Lys Ile Asp Ala Leu 
385                 390                 395                 400 


Asp His Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu Tyr His His Lys 
                405                 410                 415     


Glu Asp Lys Arg Lys Trp Glu Met Pro Thr Gly Ser Asn Glu Ser Glu 
            420                 425                 430         


Ala Lys Ser Trp Lys Ala Ile Arg Val Asp Leu Val Val Cys Pro Tyr 
        435                 440                 445             


Asp Arg Tyr Ala Phe Ala Leu Leu Gly Trp Ser Gly Ser Arg Gln Phe 
    450                 455                 460                 


Glu Arg Asp Leu Arg Arg Tyr Ala Thr His Glu Lys Lys Met Met Leu 
465                 470                 475                 480 


Asp Asn His Ala Leu Tyr Asp Lys Thr Lys Lys Ile Phe Leu Lys Ala 
                485                 490                 495     


Lys Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu Glu Tyr Ile Gln 
            500                 505                 510         


Pro Ser Glu Arg Asn Ala 
        515             


<210> 5
<211> 501
<212> PRT
<213> Oncorhynchus mykiss

<400> 5
Met Asn His Ala Gly Met Leu Ala Leu Val Lys Lys Arg Lys Arg Pro 
1               5                   10                  15      


Val Glu Ala Gly Ala Gln Gly Gln Val Glu Val Lys Phe Lys Glu Val 
            20                  25                  30          


Thr Leu Glu Leu Val Glu Arg Lys Met Gly Ser Ser Arg Arg Asn Phe 
        35                  40                  45              


Leu Thr Arg Leu Ala Arg Ser Lys Gly Phe Arg Val Glu Asp Val Leu 
    50                  55                  60                  


Ser Asp Asp Val Thr His Val Val Ala Glu Asp Asn Gln Ala Glu Val 
65                  70                  75                  80  


Leu Trp Ala Trp Leu Met Gly His Gly Leu Arg Asp Val Ser Arg Leu 
                85                  90                  95      


Ala Leu Leu Asp Ile Ser Trp Phe Thr Asp Ser Met Arg Glu Gly Arg 
            100                 105                 110         


Pro Val Arg Val Glu Thr Arg His Ser Ile Gln Asn Thr Pro Thr Gly 
        115                 120                 125             


Thr Asp Cys Ser Pro Pro Thr Ala Val Ala Asn Val Ser Gln Tyr Ala 
    130                 135                 140                 


Cys Gln Arg Arg Thr Thr Thr Glu Asn His Asn Asn Lys Ile Phe Thr 
145                 150                 155                 160 


Asp Val Met Glu Glu Leu Ala Glu Ser Ser Glu Phe Asn Glu Ser Lys 
                165                 170                 175     


Gly Pro Cys Leu Ala Phe Arg Gln Ala Ala Ser Val Leu Lys Ser Leu 
            180                 185                 190         


Pro Ser Ala Val His Cys Leu Lys Ala Ile Gln Gly Leu Pro Cys Leu 
        195                 200                 205             


Gly Glu His Thr Lys Ala Val Met Glu Glu Ile Leu Thr Phe Gly Arg 
    210                 215                 220                 


Ser Phe Lys Val Glu Glu Ile Arg Cys Asp Glu Arg Tyr Gln Ala Leu 
225                 230                 235                 240 


Lys Leu Phe Thr Ser Val Phe Gly Val Gly Pro Lys Thr Ala Glu Lys 
                245                 250                 255     


Trp Tyr Arg Arg Gly Leu Arg Ser Leu Gln Glu Ile Leu Thr Glu Pro 
            260                 265                 270         


Asn Ile Gln Leu Asn Arg Met Gln Arg Ala Gly Phe Leu Tyr Tyr Ser 
        275                 280                 285             


Asp Ile Ser Lys Ala Val Ser Lys Ala Glu Ala Lys Ala Val Gly Cys 
    290                 295                 300                 


Ile Ile Glu Asp Thr Phe His Trp Ile Ala Pro Asp Ala Ile Leu Ala 
305                 310                 315                 320 


Leu Thr Gly Gly Phe Arg Arg Gly Lys Glu Tyr Gly His Asp Val Asp 
                325                 330                 335     


Phe Leu Leu Thr Met Pro Glu Ile Gly Lys Asp Glu Gly Leu Leu Leu 
            340                 345                 350         


His Val Ile Asp Arg Leu Lys Asp Gln Gly Ile Leu Leu Tyr Cys Asp 
        355                 360                 365             


Tyr Gln Gly Ser Thr Phe Asp Val Ser Lys Leu Pro Ser Cys Arg Phe 
    370                 375                 380                 


Glu Asp Met Asp Cys Phe Gln Lys Cys Phe Leu Ile Leu Arg Leu Glu 
385                 390                 395                 400 


Gln Gly Gln Val Glu Gly Glu Arg Gly Leu Gln Arg Asp Pro Gly Asp 
                405                 410                 415     


Ser Arg Gly Trp Arg Ala Val Arg Val Asp Leu Val Ala Pro Pro Val 
            420                 425                 430         


Asp Arg Tyr Ala Phe Ala Leu Leu Gly Trp Thr Gly Ser Arg Phe Gly 
        435                 440                 445             


Arg Asp Leu Arg Thr Phe Ala Gln Lys Glu Arg Gln Met Leu Leu Asp 
    450                 455                 460                 


Asn His Ala Leu Tyr Asp Lys Thr Lys Lys Leu Cys Leu Leu Ala Thr 
465                 470                 475                 480 


Thr Glu Glu Asp Ile Phe Thr His Leu Gly Leu Glu Tyr Val Glu Pro 
                485                 490                 495     


Trp Gln Arg Asn Ala 
            500     


<210> 6
<211> 506
<212> PRT
<213> Gallus gallus

<400> 6
Met Glu Arg Ile Arg Pro Pro Thr Val Val Ser Gln Arg Lys Arg Gln 
1               5                   10                  15      


Lys Gly Met Tyr Ser Pro Lys Leu Ser Cys Gly Tyr Glu Ile Lys Phe 
            20                  25                  30          


Asn Lys Leu Val Ile Phe Ile Met Gln Arg Lys Met Gly Met Thr Arg 
        35                  40                  45              


Arg Thr Phe Leu Met Glu Leu Ala Arg Ser Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Ser Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Tyr Pro Glu Val Leu Asp Trp Leu Lys Gly Gln Ala Val Gly Asp Ser 
                85                  90                  95      


Ser Arg Phe Glu Ile Leu Asp Ile Ser Trp Leu Thr Ala Cys Met Glu 
            100                 105                 110         


Met Gly Arg Pro Val Asp Leu Glu Lys Lys Tyr His Leu Val Glu Gln 
        115                 120                 125             


Ala Gly Gln Tyr Pro Thr Leu Lys Thr Pro Glu Ser Glu Val Ser Ser 
    130                 135                 140                 


Phe Thr Ala Ser Lys Val Ser Gln Tyr Ser Cys Gln Arg Lys Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Cys Asn Lys Lys Phe Thr Asp Ala Phe Glu Ile Met Ala 
                165                 170                 175     


Glu Asn Tyr Glu Phe Lys Glu Asn Glu Ile Phe Cys Leu Glu Phe Leu 
            180                 185                 190         


Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Pro Val Thr Arg Met 
        195                 200                 205             


Lys Asp Ile Gln Gly Leu Pro Cys Met Gly Asp Arg Val Arg Asp Val 
    210                 215                 220                 


Ile Glu Glu Ile Ile Glu Glu Gly Glu Ser Ser Arg Ala Lys Asp Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Lys Ser Phe Lys Glu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Val Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Leu Arg 
            260                 265                 270         


Thr Val Glu Glu Val Lys Ala Asp Lys Thr Leu Lys Leu Ser Lys Met 
        275                 280                 285             


Gln Arg Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Ser 
    290                 295                 300                 


Lys Ala Glu Ala Asp Ala Val Ser Ser Ile Val Lys Asn Thr Val Cys 
305                 310                 315                 320 


Thr Phe Leu Pro Asp Ala Leu Val Thr Ile Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Lys Ile Gly His Asp Ile Asp Phe Leu Ile Thr Ser Pro Gly 
            340                 345                 350         


Gln Arg Glu Asp Asp Glu Leu Leu His Lys Gly Leu Leu Leu Tyr Cys 
        355                 360                 365             


Asp Ile Ile Glu Ser Thr Phe Val Lys Glu Gln Ile Pro Ser Arg His 
    370                 375                 380                 


Val Asp Ala Met Asp His Phe Gln Lys Cys Phe Ala Ile Leu Lys Leu 
385                 390                 395                 400 


Tyr Gln Pro Arg Val Asp Asn Ser Ser Tyr Asn Met Ser Lys Lys Cys 
                405                 410                 415     


Asp Met Ala Glu Val Lys Asp Trp Lys Ala Ile Arg Val Asp Leu Val 
            420                 425                 430         


Ile Thr Pro Phe Glu Gln Tyr Ala Tyr Ala Leu Leu Gly Trp Thr Gly 
        435                 440                 445             


Ser Arg Gln Phe Gly Arg Asp Leu Arg Arg Tyr Ala Thr His Glu Arg 
    450                 455                 460                 


Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys Arg Lys Arg Val 
465                 470                 475                 480 


Phe Leu Lys Ala Gly Ser Glu Glu Glu Ile Phe Ala His Leu Gly Leu 
                485                 490                 495     


Asp Tyr Val Glu Pro Trp Glu Arg Asn Ala 
            500                 505     


<210> 7
<211> 507
<212> PRT
<213> Xenopus laevis

<400> 7
Met Asn Pro Leu Ser Gln Ser Ala Leu Val Pro Leu Arg Lys Lys Ala 
1               5                   10                  15      


Lys Met Ala Pro Ile Ser Gln Ser Phe Cys Gln His Asn Val Lys Phe 
            20                  25                  30          


Lys Glu Ile Val Leu Phe Leu Val Glu Arg Lys Met Gly Ser Ser Arg 
        35                  40                  45              


Arg Thr Phe Leu Met Glu Leu Ala Arg Lys Arg Gly Phe Gln Thr Glu 
    50                  55                  60                  


Ile Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ala Glu Val Leu Glu Trp Leu Gln Ser Lys Lys Leu Gly Phe Thr 
                85                  90                  95      


Val Lys Thr His Ile Leu Asp Ile Ser Trp Phe Thr Glu Cys Met Glu 
            100                 105                 110         


Ala Gly Arg Pro Val Glu Ile Gln Asn Arg His Leu Leu Pro Val Gln 
        115                 120                 125             


Gln Asp Cys Ser Ala Asn Phe Asn Pro Pro Leu Ser Ser Ser Cys Val 
    130                 135                 140                 


Gln Val Ser Gln Tyr Ala Cys Gln Arg Cys Thr Thr Leu Gln Asp Thr 
145                 150                 155                 160 


Asn Arg Ile Phe Thr Asp Ala Phe Asp Ile Leu Ala Glu His Phe Glu 
                165                 170                 175     


Phe Cys Glu Asn Lys Gly Arg Thr Val Ala Phe Leu Arg Ala Ser Ser 
            180                 185                 190         


Leu Ile Lys Ser Leu Pro Phe Pro Ile Thr Ala Met Lys Glu Leu Glu 
        195                 200                 205             


Gly Leu Pro Trp Leu Gly Asp Gln Met Lys Gly Ile Ile Glu Glu Ile 
    210                 215                 220                 


Leu Glu Glu Gly Lys Ser Tyr Lys Val Leu Glu Val Met Asn Glu Glu 
225                 230                 235                 240 


Arg Tyr Lys Ser Phe Lys Gln Phe Thr Ser Val Phe Gly Val Gly Leu 
                245                 250                 255     


Lys Thr Ser Asp Lys Trp Phe Arg Met Gly Phe Arg Thr Leu Glu Glu 
            260                 265                 270         


Ile Lys Asn Glu Lys Glu Leu Lys Leu Thr Lys Met Gln Lys Cys Gly 
        275                 280                 285             


Leu Leu Tyr Tyr Glu Asp Ile Thr Ser Tyr Val Ser Arg Ala Glu Ala 
    290                 295                 300                 


Glu Thr Thr Glu Gln Leu Ile Lys Ser Ile Val Trp Lys Phe Val Pro 
305                 310                 315                 320 


Asp Ala Ile Val Thr Leu Thr Gly Gly Phe Arg Arg Gly Lys Lys Lys 
                325                 330                 335     


Gly His Asp Val Asp Ile Leu Ile Thr Cys Ala Arg Lys Gly Lys Glu 
            340                 345                 350         


Lys Asn Ile Leu His Asn Thr Met Ser Val Leu Lys Asn Arg Gly Leu 
        355                 360                 365             


Leu Leu Phe Tyr Asn Ile Ile Glu Ser Thr Phe Asp Glu Thr Lys Leu 
    370                 375                 380                 


Pro Ser Arg His Val Asp Ala Leu Asp His Phe Gln Lys Cys Phe Thr 
385                 390                 395                 400 


Ile Leu Lys Leu Pro Lys Arg Gln Met Asp Ile Gly Asn Ile Ile Asp 
                405                 410                 415     


Pro His Glu Cys Glu Arg Lys Asn Trp Lys Ala Val Arg Leu Asp Leu 
            420                 425                 430         


Val Ile Thr Pro Tyr Glu Gln Tyr Pro Tyr Ala Leu Leu Gly Trp Thr 
        435                 440                 445             


Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr Ala Thr His Glu 
    450                 455                 460                 


Lys Arg Met Met Leu Asp Asn His Gly Leu Tyr Asp Lys Thr Lys Asn 
465                 470                 475                 480 


Asn Phe Leu Lys Ala Asn Asn Glu Glu Asp Ile Phe Lys Gln Leu Gly 
                485                 490                 495     


Leu Asp Tyr Leu Glu Pro Trp Glu Arg Asn Ala 
            500                 505         


<210> 8
<211> 509
<212> PRT
<213> Bos taurus

<400> 8
Met Asp Pro Leu Cys Thr Ala Ser Ser Gly Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Val Gly Ala Ser Met Ala Ser Pro Pro His Asp Ile Lys Phe 
            20                  25                  30          


Gln Asn Leu Val Leu Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg 
        35                  40                  45              


Arg Asn Phe Leu Met Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Glu Val Leu Glu Trp Leu Gln Val Gln Asn Ile Arg Ala Ser 
                85                  90                  95      


Ser Gln Leu Glu Leu Leu Asp Val Ser Trp Leu Ile Glu Ser Met Gly 
            100                 105                 110         


Ala Gly Lys Pro Val Glu Ile Thr Gly Lys His Gln Leu Val Val Arg 
        115                 120                 125             


Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro 
    130                 135                 140                 


Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala 
                165                 170                 175     


Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe Met 
            180                 185                 190         


Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met 
        195                 200                 205             


Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile 
    210                 215                 220                 


Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys Met 
        275                 280                 285             


Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr 
    290                 295                 300                 


Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val Trp 
305                 310                 315                 320 


Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly 
            340                 345                 350         


Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp 
        355                 360                 365             


Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe 
    370                 375                 380                 


Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe 
385                 390                 395                 400 


Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp Ser 
                405                 410                 415     


Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val 
            420                 425                 430         


Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly 
        435                 440                 445             


Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr 
    450                 455                 460                 


His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr 
465                 470                 475                 480 


Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His 
                485                 490                 495     


Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 505                 


<210> 9
<211> 510
<212> PRT
<213> Mus musculus

<400> 9
Met Asp Pro Leu Gln Ala Val His Leu Gly Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Leu Gly Thr Pro Val Ala Ser Thr Pro Tyr Asp Ile Arg Phe 
            20                  25                  30          


Arg Asp Leu Val Leu Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg 
        35                  40                  45              


Arg Ala Phe Leu Met Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Asp Val Leu Glu Trp Leu Gln Leu Gln Asn Ile Lys Ala Ser 
                85                  90                  95      


Ser Glu Leu Glu Leu Leu Asp Ile Ser Trp Leu Ile Glu Cys Met Gly 
            100                 105                 110         


Ala Gly Lys Pro Val Glu Met Met Gly Arg His Gln Leu Val Val Asn 
        115                 120                 125             


Arg Asn Ser Ser Pro Ser Pro Val Pro Gly Ser Gln Asn Val Pro Ala 
    130                 135                 140                 


Pro Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Arg Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Tyr Asn Gln Leu Phe Thr Asp Ala Leu Asp Ile Leu Ala 
                165                 170                 175     


Glu Asn Asp Glu Leu Arg Glu Asn Glu Gly Ser Cys Leu Ala Phe Met 
            180                 185                 190         


Arg Ala Ser Ser Val Leu Lys Ser Leu Pro Phe Pro Ile Thr Ser Met 
        195                 200                 205             


Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Ser Ile 
    210                 215                 220                 


Ile Glu Gly Ile Ile Glu Asp Gly Glu Ser Ser Glu Ala Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Lys Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ala Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Thr Leu Ser Lys Ile Gln Ser Asp Lys Ser Leu Arg Phe Thr Gln Met 
        275                 280                 285             


Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Asn 
    290                 295                 300                 


Arg Pro Glu Ala Glu Ala Val Ser Met Leu Val Lys Glu Ala Val Val 
305                 310                 315                 320 


Thr Phe Leu Pro Asp Ala Leu Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Met Thr Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Glu 
            340                 345                 350         


Ala Thr Glu Asp Glu Glu Gln Gln Leu Leu His Lys Val Thr Asp Phe 
        355                 360                 365             


Trp Lys Gln Gln Gly Leu Leu Leu Tyr Cys Asp Ile Leu Glu Ser Thr 
    370                 375                 380                 


Phe Glu Lys Phe Lys Gln Pro Ser Arg Lys Val Asp Ala Leu Asp His 
385                 390                 395                 400 


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu Asp His Gly Arg Val His 
                405                 410                 415     


Ser Glu Lys Ser Gly Gln Gln Glu Gly Lys Gly Trp Lys Ala Ile Arg 
            420                 425                 430         


Val Asp Leu Val Met Cys Pro Tyr Asp Arg Arg Ala Phe Ala Leu Leu 
        435                 440                 445             


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr Ala 
    450                 455                 460                 


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Arg 
465                 470                 475                 480 


Thr Lys Arg Val Phe Leu Glu Ala Glu Ser Glu Glu Glu Ile Phe Ala 
                485                 490                 495     


His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 505                 510 


<210> 10
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
tttttttttt ttttt                                                        15


<210> 11
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
aaaaaaaaaa aaaaa                                                        15

