                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Samulski, Richard Jude
 
<120>  REGULATED GENE EDITING SYSTEM

<130>  5470-858WO

<150>  62/870,427
<151>  2019-07-03

<150>  62/743,317
<151>  2018-10-09

<160>  154   

<170>  PatentIn version 3.5

<210>  1
<211>  7713
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid TRCBA-int-luc mut (654 C-T)


<220>
<221>  Intron
<222>  (2739)..(3588)

<400>  1
gggggggggg gggggggttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg       60

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      120

cgcgcagaga gggagtggcc aactccatca ctaggggttc ctagatcttc aatattggcc      180

attagccata ttattcattg gttatatagc ataaatcaat attggatatt ggccattgca      240

tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc      300

atgttggcat tgattattga ctagttatta atagtaatca attacggggt cattagttca      360

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc      420

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat      480

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      540

acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc      600

cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta      660

cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc      720

catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc      780

agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg      840

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa      900

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg      960

ggcgggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     1020

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     1080

ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc     1140

gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt     1200

gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg     1260

agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg     1320

ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg     1380

gtgtgtgcgt gggggggtga gcagggggta tgggcgcggc ggtcgggctg taaccccccc     1440

ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg     1500

gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc     1560

ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc     1620

gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag     1680

agggcgcagg gacttacttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc     1740

cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1800

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc     1860

tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg     1920

cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta     1980

cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattagct     2040

tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc     2100

ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg     2160

aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac     2220

atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat     2280

gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg     2340

ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat     2400

gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa     2460

aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt     2520

atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct     2580

catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag     2640

acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg     2700

cctcatagaa ctgcctgcgt gagattctcg catgccaggt gagtctatgg gacccttgat     2760

gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca     2820

gggtacagtt tagaatggga aacagacgaa tgattgcatc agtgtggaag tctcaggatc     2880

gttttagttt cttttatttg ctgttcataa caattgtttt cttttgttta attcttgctt     2940

tctttttttt tcttctccgc aatttttact attatactta atgccttaac attgtgtata     3000

acaaaaggaa atatctctga gatacattaa gtaacttaaa aaaaaacttt acacagtctg     3060

cctagtacat tactatttgg aatatatgtg tgcttatttg catattcata atctccctac     3120

tttattttct tttattttta attgatacat aatcattata catatttatg ggttaaagtg     3180

taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca tttgtaattt     3240

taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc     3300

taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta     3360

aagaataaca gtgataattt ctgggttaag gtaatagcaa tatttctgca tataaatatt     3420

tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc     3480

agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc     3540

taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagag atcctatttt     3600

tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt     3660

tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag     3720

atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct     3780

ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc     3840

taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt     3900

tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc     3960

agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc     4020

attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag     4080

aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc     4140

gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga     4200

cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta     4260

tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc     4320

aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt     4380

ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac     4440

aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac     4500

cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa     4560

gatcgccgtg taattctagg gccgcttcga gcagacatga taagatacat tgatgagttt     4620

ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct     4680

attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt     4740

cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc     4800

tacaaatgtg gtaaaatcga taaggatcta ggaaccccta gtgatggagt tggccactcc     4860

ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac     4920

ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaacccccc     4980

cccccccccc cctgcagcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca     5040

acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc     5100

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     5160

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     5220

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     5280

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     5340

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     5400

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     5460

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt     5520

tacaatttcc tgatgcgcta ttttctcctt acgcatctgt gcggtatttc acaccgcata     5580

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg     5640

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa     5700

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc     5760

gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg     5820

gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     5880

tttttctaaa tactttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     5940

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc     6000

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa     6060

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt     6120

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt     6180

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc     6240

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg     6300

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg     6360

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac     6420

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca     6480

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta     6540

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat     6600

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgcggataaa     6660

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag     6720

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat     6780

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt     6840

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg     6900

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga     6960

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta     7020

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa     7080

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact     7140

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca     7200

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt     7260

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     7320

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     7380

cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     7440

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     7500

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     7560

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     7620

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     7680

cgtattaccg cctttgagtg agctgatacc gct                                  7713


<210>  2
<211>  7713
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid TRCBA-int-luc (wt)


<220>
<221>  Intron
<222>  (2739)..(3588)

<400>  2
gggggggggg gggggggttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg       60

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      120

cgcgcagaga gggagtggcc aactccatca ctaggggttc ctagatcttc aatattggcc      180

attagccata ttattcattg gttatatagc ataaatcaat attggatatt ggccattgca      240

tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc      300

atgttggcat tgattattga ctagttatta atagtaatca attacggggt cattagttca      360

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc      420

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat      480

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      540

acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc      600

cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta      660

cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc      720

catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc      780

agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg      840

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa      900

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg      960

ggcgggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     1020

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     1080

ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc     1140

gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt     1200

gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg     1260

agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg     1320

ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg     1380

gtgtgtgcgt gggggggtga gcagggggta tgggcgcggc ggtcgggctg taaccccccc     1440

ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg     1500

gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc     1560

ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc     1620

gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag     1680

agggcgcagg gacttacttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc     1740

cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1800

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc     1860

tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg     1920

cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta     1980

cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattagct     2040

tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc     2100

ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg     2160

aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac     2220

atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat     2280

gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg     2340

ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat     2400

gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa     2460

aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt     2520

atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct     2580

catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag     2640

acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg     2700

cctcatagaa ctgcctgcgt gagattctcg catgccaggt gagtctatgg gacccttgat     2760

gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca     2820

gggtacagtt tagaatggga aacagacgaa tgattgcatc agtgtggaag tctcaggatc     2880

gttttagttt cttttatttg ctgttcataa caattgtttt cttttgttta attcttgctt     2940

tctttttttt tcttctccgc aatttttact attatactta atgccttaac attgtgtata     3000

acaaaaggaa atatctctga gatacattaa gtaacttaaa aaaaaacttt acacagtctg     3060

cctagtacat tactatttgg aatatatgtg tgcttatttg catattcata atctccctac     3120

tttattttct tttattttta attgatacat aatcattata catatttatg ggttaaagtg     3180

taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca tttgtaattt     3240

taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc     3300

taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta     3360

aagaataaca gtgataattt ctgggttaag gcaatagcaa tatttctgca tataaatatt     3420

tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc     3480

agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc     3540

taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagag atcctatttt     3600

tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt     3660

tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag     3720

atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct     3780

ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc     3840

taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt     3900

tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc     3960

agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc     4020

attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag     4080

aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc     4140

gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga     4200

cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta     4260

tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc     4320

aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt     4380

ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac     4440

aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac     4500

cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa     4560

gatcgccgtg taattctagg gccgcttcga gcagacatga taagatacat tgatgagttt     4620

ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct     4680

attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt     4740

cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc     4800

tacaaatgtg gtaaaatcga taaggatcta ggaaccccta gtgatggagt tggccactcc     4860

ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac     4920

ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaacccccc     4980

cccccccccc cctgcagcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca     5040

acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc     5100

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     5160

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     5220

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     5280

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     5340

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     5400

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     5460

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt     5520

tacaatttcc tgatgcgcta ttttctcctt acgcatctgt gcggtatttc acaccgcata     5580

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg     5640

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa     5700

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc     5760

gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg     5820

gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     5880

tttttctaaa tactttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     5940

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc     6000

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa     6060

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt     6120

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt     6180

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc     6240

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg     6300

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg     6360

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac     6420

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca     6480

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta     6540

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat     6600

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgcggataaa     6660

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag     6720

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat     6780

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt     6840

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg     6900

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga     6960

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta     7020

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa     7080

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact     7140

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca     7200

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt     7260

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     7320

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     7380

cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     7440

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     7500

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     7560

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     7620

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     7680

cgtattaccg cctttgagtg agctgatacc gct                                  7713


<210>  3
<211>  7713
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid TRCBA-int-luc (654 C-T, 657 TA-GT)


<220>
<221>  Intron
<222>  (2739)..(3588)

<400>  3
gggggggggg gggggggttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg       60

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      120

cgcgcagaga gggagtggcc aactccatca ctaggggttc ctagatcttc aatattggcc      180

attagccata ttattcattg gttatatagc ataaatcaat attggatatt ggccattgca      240

tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc      300

atgttggcat tgattattga ctagttatta atagtaatca attacggggt cattagttca      360

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc      420

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat      480

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      540

acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc      600

cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta      660

cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc      720

catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc      780

agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg      840

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa      900

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg      960

ggcgggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     1020

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     1080

ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc     1140

gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt     1200

gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg     1260

agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg     1320

ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg     1380

gtgtgtgcgt gggggggtga gcagggggta tgggcgcggc ggtcgggctg taaccccccc     1440

ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg     1500

gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc     1560

ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc     1620

gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag     1680

agggcgcagg gacttacttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc     1740

cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1800

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc     1860

tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg     1920

cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta     1980

cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattagct     2040

tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc     2100

ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg     2160

aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac     2220

atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat     2280

gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg     2340

ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat     2400

gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa     2460

aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt     2520

atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct     2580

catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag     2640

acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg     2700

cctcatagaa ctgcctgcgt gagattctcg catgccaggt gagtctatgg gacccttgat     2760

gttttctttc cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca     2820

gggtacagtt tagaatggga aacagacgaa tgattgcatc agtgtggaag tctcaggatc     2880

gttttagttt cttttatttg ctgttcataa caattgtttt cttttgttta attcttgctt     2940

tctttttttt tcttctccgc aatttttact attatactta atgccttaac attgtgtata     3000

acaaaaggaa atatctctga gatacattaa gtaacttaaa aaaaaacttt acacagtctg     3060

cctagtacat tactatttgg aatatatgtg tgcttatttg catattcata atctccctac     3120

tttattttct tttattttta attgatacat aatcattata catatttatg ggttaaagtg     3180

taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca tttgtaattt     3240

taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta atactttccc     3300

taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg caccattcta     3360

aagaataaca gtgataattt ctgggttaag gcaagtgcaa tatttctgca tataaatatt     3420

tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca gctacaatcc     3480

agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc     3540

taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagag atcctatttt     3600

tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt     3660

tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag     3720

atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct     3780

ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc     3840

taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt     3900

tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc     3960

agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc     4020

attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag     4080

aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc     4140

gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga     4200

cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta     4260

tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc     4320

aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt     4380

ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac     4440

aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac     4500

cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa     4560

gatcgccgtg taattctagg gccgcttcga gcagacatga taagatacat tgatgagttt     4620

ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct     4680

attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt     4740

cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc     4800

tacaaatgtg gtaaaatcga taaggatcta ggaaccccta gtgatggagt tggccactcc     4860

ctctctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac     4920

ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaacccccc     4980

cccccccccc cctgcagcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca     5040

acagttgcgt agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc     5100

ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc     5160

tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct     5220

aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa     5280

acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc     5340

tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact     5400

caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg     5460

gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt     5520

tacaatttcc tgatgcgcta ttttctcctt acgcatctgt gcggtatttc acaccgcata     5580

tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg     5640

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa     5700

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc     5760

gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg     5820

gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta     5880

tttttctaaa tactttcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt     5940

caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc     6000

ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa     6060

gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt     6120

aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt     6180

ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc     6240

atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg     6300

gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg     6360

gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac     6420

atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca     6480

aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta     6540

actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat     6600

aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgcggataaa     6660

tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag     6720

ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat     6780

agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt     6840

tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg     6900

aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga     6960

gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta     7020

atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa     7080

gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact     7140

gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca     7200

tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt     7260

accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg     7320

ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag     7380

cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta     7440

agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat     7500

ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg     7560

tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc     7620

ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac     7680

cgtattaccg cctttgagtg agctgatacc gct                                  7713


<210>  4
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-Luc mut (654 C-T)


<220>
<221>  Intron
<222>  (948)..(1797)

<400>  4
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc ttttgtttaa     1140

ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa tgccttaaca     1200

ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa aaaaacttta     1260

cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc atattcataa     1320

tctccctact ttattttctt ttatttttaa ttgatacata atcattatac atatttatgg     1380

gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt aattttgcat     1440

ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa     1500

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc     1560

accattctaa agaataacag tgataatttc tgggttaagg taatagcaat atttctgcat     1620

ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag     1680

ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg     1740

agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagaga     1800

tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca     1860

tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt     1920

aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag     1980

tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata     2040

cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg     2100

ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga     2160

gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa     2220

agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt     2280

taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  5
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-Luc (wt)


<220>
<221>  Intron
<222>  (948)..(1797)

<400>  5
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc ttttgtttaa     1140

ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa tgccttaaca     1200

ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa aaaaacttta     1260

cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc atattcataa     1320

tctccctact ttattttctt ttatttttaa ttgatacata atcattatac atatttatgg     1380

gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt aattttgcat     1440

ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa     1500

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc     1560

accattctaa agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat     1620

ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag     1680

ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg     1740

agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagaga     1800

tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca     1860

tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt     1920

aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag     1980

tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata     2040

cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg     2100

ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga     2160

gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa     2220

agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt     2280

taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  6
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-Luc (654 C-T, 657 TA-GT)


<220>
<221>  Intron
<222>  (48)..(1797)

<400>  6
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc ttttgtttaa     1140

ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa tgccttaaca     1200

ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa aaaaacttta     1260

cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc atattcataa     1320

tctccctact ttattttctt ttatttttaa ttgatacata atcattatac atatttatgg     1380

gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt aattttgcat     1440

ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa     1500

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc     1560

accattctaa agaataacag tgataatttc tgggttaagg taagtgcaat atttctgcat     1620

ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag     1680

ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg     1740

agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagaga     1800

tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca     1860

tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt     1920

aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag     1980

tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata     2040

cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg     2100

ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga     2160

gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa     2220

agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt     2280

taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  7
<211>  6683
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-2int-fron-sph (mut)


<220>
<221>  Intron
<222>  (251)..(1100)

<220>
<221>  Intron
<222>  (1771)..(2620)

<400>  7
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt      300

aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg      360

aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat      420

aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta      480

ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt      540

aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg      600

tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt taattgatac      660

ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg      720

accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac      780

ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga      840

tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta      900

aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag      960

aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt     1020

tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac     1080

ctcttatctt cctcccacag ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc     1140

attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata     1200

cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta     1260

cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa     1320

tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt     1380

gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga     1440

attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt     1500

gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga     1560

ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc     1620

tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc     1680

actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag     1740

aactgcctgc gtgagattct cgcatgccag gtgagtctat gggacccttg atgttttctt     1800

tccccttctt ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag     1860

tttagaatgg gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt     1920

ttcttttatt tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt     1980

tttcttctcc gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg     2040

aaatatctct gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac     2100

attactattt ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt     2160

cttttatttt taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt     2220

taatatgtgt acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat     2280

gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct     2340

ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa     2400

cagtgataat ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata     2460

taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca     2520

ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct     2580

tttgctaatc atgttcatac ctcttatctt cctcccacag agatcctatt tttggcaatc     2640

aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt     2700

ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag     2760

aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa     2820

ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac     2880

acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga     2940

ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc     3000

tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg     3060

aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac     3120

tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg     3180

ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg     3240

aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg     3300

ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg     3360

caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg     3420

gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga     3480

aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac     3540

tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg     3600

tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg     3660

agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg     3720

atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt     3780

gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa     3840

acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc     3900

aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact     3960

gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct     4020

cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     4080

ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     4140

ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     4200

cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     4260

actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     4320

cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     4380

tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     4440

gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     4500

caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     4560

agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     4620

tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     4680

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     4740

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     4800

gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     4860

aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     4920

atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     4980

gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     5040

acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     5100

ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     5160

tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     5220

ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     5280

ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     5340

atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     5400

taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     5460

catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     5520

atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     5580

acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     5640

aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     5700

ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     5760

cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     5820

atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     5880

ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc     5940

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac     6000

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt     6060

cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc     6120

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc     6180

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact     6240

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg     6300

gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc     6360

gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg     6420

ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata     6480

agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt     6540

tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat     6600

caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt     6660

gccagaacat ttctctatcg ata                                             6683


<210>  8
<211>  7547
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-3int-2fron-sph (mut)


<220>
<221>  Intron
<222>  (251)..(1100)

<220>
<221>  Intron
<222>  (1111)..(1960)

<220>
<221>  Intron
<222>  (2635)..(3484)

<400>  8
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt      300

aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg      360

aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat      420

aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta      480

ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt      540

aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg      600

tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt taattgatac      660

ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg      720

accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac      780

ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga      840

tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta      900

aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag      960

aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt     1020

tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac     1080

ctcttatctt cctcccacag ccatgagctt gtgagtctat gggacccttg atgttttctt     1140

tccccttctt ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag     1200

tttagaatgg gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt     1260

ttcttttatt tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt     1320

tttcttctcc gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg     1380

aaatatctct gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac     1440

attactattt ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt     1500

cttttatttt taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt     1560

taatatgtgt acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat     1620

gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct     1680

ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa     1740

cagtgataat ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata     1800

taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca     1860

ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct     1920

tttgctaatc atgttcatac ctcttatctt cctcccacag ccatgcatgg aagacgccaa     1980

aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca     2040

actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc     2100

acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga     2160

agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc     2220

tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc     2280

gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt     2340

ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat     2400

catccaaaaa attattatca tggattctaa aacggattac cagggatttc agtcgatgta     2460

cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc     2520

cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc     2580

taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccaggtgagt     2640

ctatgggacc cttgatgttt tctttcccct tcttttctat ggttaagttc atgtcatagg     2700

aaggggagaa gtaacagggt acagtttaga atgggaaaca gacgaatgat tgcatcagtg     2760

tggaagtctc aggatcgttt tagtttcttt tatttgctgt tcataacaat tgttttcttt     2820

tgtttaattc ttgctttctt tttttttctt ctccgcaatt tttactatta tacttaatgc     2880

cttaacattg tgtataacaa aaggaaatat ctctgagata cattaagtaa cttaaaaaaa     2940

aactttacac agtctgccta gtacattact atttggaata tatgtgtgct tatttgcata     3000

ttcataatct ccctacttta ttttctttta tttttaattg atacataatc attatacata     3060

tttatgggtt aaagtgtaat gttttaatat gtgtacacat attgaccaaa tcagggtaat     3120

tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta     3180

tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc     3240

tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggtaa tagcaatatt     3300

tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt catattgcta     3360

atagcagcta caatccagct accattctgc ttttatttta tggttgggat aaggctggat     3420

tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc     3480

acagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc     3540

cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag     3600

tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga     3660

ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg     3720

acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg     3780

aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc     3840

tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg     3900

tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc     3960

tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg     4020

taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag     4080

acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga     4140

ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc     4200

ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg     4260

ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg     4320

tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag     4380

taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg     4440

ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag     4500

cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa     4560

aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca     4620

ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt     4680

gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt     4740

cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg     4800

actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg     4860

gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     4920

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     4980

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     5040

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     5100

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     5160

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     5220

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     5280

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     5340

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     5400

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     5460

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa     5520

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     5580

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     5640

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     5700

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     5760

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     5820

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     5880

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     5940

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     6000

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     6060

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     6120

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     6180

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt     6240

cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc     6300

agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga     6360

gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc     6420

gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa     6480

acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta     6540

acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg     6600

agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg     6660

aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat     6720

gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt     6780

tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt     6840

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt     6900

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct     6960

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg     7020

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga     7080

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc     7140

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga     7200

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca     7260

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     7320

acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg tacttggagc     7380

ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg     7440

atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag     7500

gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata                   7547


<210>  9
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-luc A (mut)


<220>
<221>  Intron
<222>  (673)..(1522)

<400>  9
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggtgagtct atgggaccct tgatgttttc tttccccttc ttttctatgg      720

ttaagttcat gtcataggaa ggggagaagt aacagggtac agtttagaat gggaaacaga      780

cgaatgattg catcagtgtg gaagtctcag gatcgtttta gtttctttta tttgctgttc      840

ataacaattg ttttcttttg tttaattctt gctttctttt tttttcttct ccgcaatttt      900

tactattata cttaatgcct taacattgtg tataacaaaa ggaaatatct ctgagataca      960

ttaagtaact taaaaaaaaa ctttacacag tctgcctagt acattactat ttggaatata     1020

tgtgtgctta tttgcatatt cataatctcc ctactttatt ttcttttatt tttaattgat     1080

acataatcat tatacatatt tatgggttaa agtgtaatgt tttaatatgt gtacacatat     1140

tgaccaaatc agggtaattt tgcatttgta attttaaaaa atgctttctt cttttaatat     1200

acttttttgt ttatcttatt tctaatactt tccctaatct ctttctttca gggcaataat     1260

gatacaatgt atcatgcctc tttgcaccat tctaaagaat aacagtgata atttctgggt     1320

taaggtaata gcaatatttc tgcatataaa tatttctgca tataaattgt aactgatgta     1380

agaggtttca tattgctaat agcagctaca atccagctac cattctgctt ttattttatg     1440

gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa tcatgttcat     1500

acctcttatc ttcctcccac aggggttgca aaaaattttg aacgtgcaaa aaaagctccc     1560

aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat     1620

gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga     1680

gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct     1740

gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga     1800

tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca     1860

tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt     1920

aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag     1980

tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata     2040

cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg     2100

ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga     2160

gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa     2220

agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt     2280

taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  10
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-Luc B


<220>
<221>  Intron
<222>  (1440)..(2289)

<400>  10
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt      960

ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt     1020

ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga     1080

tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg     1140

gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct     1200

aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt     1260

gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca     1320

gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca     1380

ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaagg     1440

tgagtctatg ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc     1500

ataggaaggg gagaagtaac agggtacagt ttagaatggg aaacagacga atgattgcat     1560

cagtgtggaa gtctcaggat cgttttagtt tcttttattt gctgttcata acaattgttt     1620

tcttttgttt aattcttgct ttcttttttt ttcttctccg caatttttac tattatactt     1680

aatgccttaa cattgtgtat aacaaaagga aatatctctg agatacatta agtaacttaa     1740

aaaaaaactt tacacagtct gcctagtaca ttactatttg gaatatatgt gtgcttattt     1800

gcatattcat aatctcccta ctttattttc ttttattttt aattgataca taatcattat     1860

acatatttat gggttaaagt gtaatgtttt aatatgtgta cacatattga ccaaatcagg     1920

gtaattttgc atttgtaatt ttaaaaaatg ctttcttctt ttaatatact tttttgttta     1980

tcttatttct aatactttcc ctaatctctt tctttcaggg caataatgat acaatgtatc     2040

atgcctcttt gcaccattct aaagaataac agtgataatt tctgggttaa ggtaatagca     2100

atatttctgc atataaatat ttctgcatat aaattgtaac tgatgtaaga ggtttcatat     2160

tgctaatagc agctacaatc cagctaccat tctgctttta ttttatggtt gggataaggc     2220

tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc tcttatcttc     2280

ctcccacaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  11
<211>  5860
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-Luc C


<220>
<221>  Intron
<222>  (1691)..(2540)

<400>  11
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt      960

ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt     1020

ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga     1080

tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg     1140

gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct     1200

aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt     1260

gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca     1320

gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca     1380

ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga     1440

ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg     1500

accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac     1560

gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat     1620

caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca     1680

ggtgtcgcag gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt     1740

aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg     1800

aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat     1860

aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta     1920

ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt     1980

aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg     2040

tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt taattgatac     2100

ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg     2160

accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac     2220

ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga     2280

tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta     2340

aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag     2400

aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt     2460

tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac     2520

ctcttatctt cctcccacag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt     2580

tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag     2640

tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa     2700

aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa     2760

gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat     2820

gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt     2880

tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca     2940

agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt     3000

tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga     3060

tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg     3120

tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc     3180

tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     3240

tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     3300

aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     3360

tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     3420

tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     3480

cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     3540

agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     3600

tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     3660

aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     3720

ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     3780

cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt     3840

accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     3900

ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     3960

ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     4020

gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     4080

aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     4140

gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     4200

gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     4260

cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     4320

gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     4380

gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     4440

ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     4500

tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     4560

ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     4620

cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     4680

accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     4740

cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     4800

tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     4860

cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     4920

acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     4980

atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     5040

tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     5100

aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     5160

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     5220

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     5280

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     5340

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     5400

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     5460

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt     5520

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca     5580

ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag     5640

cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca     5700

ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac     5760

taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc     5820

ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                           5860


<210>  12
<211>  5833
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-int-fron (mut)


<220>
<221>  Intron
<222>  (251)..(1100)

<400>  12
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt      300

aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg      360

aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat      420

aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta      480

ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt      540

aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg      600

tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt taattgatac      660

ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg      720

accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac      780

ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga      840

tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta      900

aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag      960

aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt     1020

tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac     1080

ctcttatctt cctcccacag ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc     1140

attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata     1200

cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta     1260

cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa     1320

tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt     1380

gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga     1440

attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt     1500

gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga     1560

ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc     1620

tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc     1680

actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag     1740

aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc     1800

ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact     1860

cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt     1920

tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc     1980

cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc     2040

ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct     2100

gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc     2160

cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt     2220

tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag     2280

aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga     2340

caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt     2400

catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga     2460

attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc     2520

cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat     2580

gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg     2640

cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag     2700

aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta     2760

gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca     2820

aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc     2880

tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt     2940

tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa     3000

atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca     3060

gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta     3120

tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg     3180

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     3240

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     3300

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     3360

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     3420

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     3480

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     3540

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     3600

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     3660

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     3720

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     3780

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     3840

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     3900

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     3960

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     4020

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     4080

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     4140

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     4200

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     4260

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     4320

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     4380

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     4440

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     4500

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     4560

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     4620

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     4680

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     4740

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     4800

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     4860

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     4920

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     4980

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     5040

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc     5100

ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc     5160

gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt     5220

ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac     5280

ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag     5340

acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa     5400

actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg     5460

atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac     5520

aaaatattaa cgcttacaat ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg     5580

cgatcggtgc gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat     5640

attaaggtac gggaggtact tggagcggcc gcaataaaat atctttattt tcattacatc     5700

tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa     5760

cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat     5820

ttctctatcg ata                                                        5833


<210>  13
<211>  6710
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-2int-sph (mut)


<220>
<221>  Intron
<222>  (948)..(1797)

<220>
<221>  Intron
<222>  (1798)..(2647)

<400>  13
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc ttttgtttaa     1140

ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa tgccttaaca     1200

ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa aaaaacttta     1260

cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc atattcataa     1320

tctccctact ttattttctt ttatttttaa ttgatacata atcattatac atatttatgg     1380

gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt aattttgcat     1440

ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa     1500

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc     1560

accattctaa agaataacag tgataatttc tgggttaagg taatagcaat atttctgcat     1620

ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag     1680

ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg     1740

agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacaggtg     1800

agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat     1860

aggaagggga gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca     1920

gtgtggaagt ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc     1980

ttttgtttaa ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa     2040

tgccttaaca ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa     2100

aaaaacttta cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc     2160

atattcataa tctccctact ttattttctt ttatttttaa ttgatacata atcattatac     2220

atatttatgg gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt     2280

aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc     2340

ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat     2400

gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg taatagcaat     2460

atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg     2520

ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg     2580

gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct     2640

cccacagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg     2700

ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc     2760

gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca     2820

agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga     2880

ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta     2940

aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg     3000

ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg     3060

cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa     3120

cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt     3180

atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg     3240

gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc     3300

tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac     3360

accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc     3420

ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt     3480

acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg     3540

aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa     3600

aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc     3660

gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa     3720

aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct     3780

gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg     3840

tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc     3900

cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc     3960

atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg     4020

ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     4080

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     4140

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     4200

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     4260

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     4320

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     4380

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     4440

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     4500

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     4560

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     4620

gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct     4680

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     4740

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     4800

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     4860

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     4920

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     4980

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     5040

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     5100

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     5160

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     5220

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     5280

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     5340

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     5400

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     5460

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     5520

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     5580

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     5640

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     5700

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     5760

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     5820

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     5880

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     5940

atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt     6000

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc     6060

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg     6120

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta     6180

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt     6240

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat     6300

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa     6360

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg     6420

ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct     6480

attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg     6540

agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa     6600

tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa     6660

taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                6710


<210>  14
<211>  6710
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-2int-Sph-C


<220>
<221>  Intron
<222>  (948)..(1797)

<220>
<221>  Intron
<222>  (2541)..(3390)

<400>  14
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagtttc ttttatttgc tgttcataac aattgttttc ttttgtttaa     1140

ttcttgcttt cttttttttt cttctccgca atttttacta ttatacttaa tgccttaaca     1200

ttgtgtataa caaaaggaaa tatctctgag atacattaag taacttaaaa aaaaacttta     1260

cacagtctgc ctagtacatt actatttgga atatatgtgt gcttatttgc atattcataa     1320

tctccctact ttattttctt ttatttttaa ttgatacata atcattatac atatttatgg     1380

gttaaagtgt aatgttttaa tatgtgtaca catattgacc aaatcagggt aattttgcat     1440

ttgtaatttt aaaaaatgct ttcttctttt aatatacttt tttgtttatc ttatttctaa     1500

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc     1560

accattctaa agaataacag tgataatttc tgggttaagg taatagcaat atttctgcat     1620

ataaatattt ctgcatataa attgtaactg atgtaagagg tttcatattg ctaatagcag     1680

ctacaatcca gctaccattc tgcttttatt ttatggttgg gataaggctg gattattctg     1740

agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct cccacagaga     1800

tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca     1860

tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt     1920

aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag     1980

tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata     2040

cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg     2100

ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga     2160

gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa     2220

agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt     2280

taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa     2340

tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc     2400

ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta     2460

caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat     2520

cttcgacgca ggtgtcgcag gtgagtctat gggacccttg atgttttctt tccccttctt     2580

ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg     2640

gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt     2700

tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc     2760

gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct     2820

gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt     2880

ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt     2940

taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt     3000

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct     3060

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg     3120

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat     3180

ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa     3240

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt     3300

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc     3360

atgttcatac ctcttatctt cctcccacag gtcttcccga cgatgacgcc ggtgaacttc     3420

ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt     3480

acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg     3540

aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa     3600

aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc     3660

gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa     3720

aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct     3780

gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg     3840

tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc     3900

cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc     3960

atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg     4020

ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     4080

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     4140

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     4200

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     4260

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     4320

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     4380

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     4440

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     4500

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     4560

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     4620

gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct     4680

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     4740

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     4800

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     4860

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     4920

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     4980

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     5040

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     5100

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     5160

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     5220

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     5280

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     5340

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     5400

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     5460

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     5520

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     5580

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     5640

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     5700

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     5760

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     5820

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     5880

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     5940

atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt     6000

ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc     6060

tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg     6120

gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta     6180

gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt     6240

ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat     6300

ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa     6360

tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg     6420

ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct     6480

attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg     6540

agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa     6600

tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa     6660

taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata                6710


<210>  15
<211>  5660
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-sint200-sph (mut)


<220>
<221>  Intron
<222>  (948)..(1597)

<400>  15
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagttgt gcttatttgc atattcataa tctccctact ttattttctt     1140

ttatttttaa ttgatacata atcattatac atatttatgg gttaaagtgt aatgttttaa     1200

tatgtgtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct     1260

ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc     1320

tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag     1380

tgataatttc tgggttaagg taatagcaat atttctgcat ataaatattt ctgcatataa     1440

attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc     1500

tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt     1560

gctaatcatg ttcatacctc ttatcttcct cccacagaga tcctattttt ggcaatcaaa     1620

tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta     1680

ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag     1740

agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc     1800

tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg     1860

aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt     1920

tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga     1980

ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag     2040

cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt     2100

gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct     2160

tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac     2220

acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc     2280

ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag     2340

gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa     2400

agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa     2460

agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg     2520

acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt     2580

aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     2640

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     2700

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     2760

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     2820

tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac     2880

ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc     2940

ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac     3000

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     3060

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     3120

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     3180

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     3240

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     3300

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     3360

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     3420

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     3480

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     3540

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     3600

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     3660

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     3720

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     3780

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     3840

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     3900

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     3960

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     4020

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     4080

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     4140

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     4200

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     4260

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     4320

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     4380

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     4440

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     4500

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     4560

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     4620

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     4680

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     4740

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     4800

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     4860

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc     4920

ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact     4980

tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc     5040

cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt     5100

acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc     5160

ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt     5220

gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat     5280

tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa     5340

ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg     5400

ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt     5460

aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca     5520

ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa     5580

aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc     5640

agaacatttc tctatcgata                                                 5660


<210>  16
<211>  5660
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-sint200-sph (657 GT)


<220>
<221>  Intron
<222>  (948)..(1597)

<400>  16
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga     1020

gaagtaacag ggtacagttt agaatgggaa acagacgaat gattgcatca gtgtggaagt     1080

ctcaggatcg ttttagttgt gcttatttgc atattcataa tctccctact ttattttctt     1140

ttatttttaa ttgatacata atcattatac atatttatgg gttaaagtgt aatgttttaa     1200

tatgtgtaca catattgacc aaatcagggt aattttgcat ttgtaatttt aaaaaatgct     1260

ttcttctttt aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc     1320

tttcagggca ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag     1380

tgataatttc tgggttaagg taagtgcaat atttctgcat ataaatattt ctgcatataa     1440

attgtaactg atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc     1500

tgcttttatt ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt     1560

gctaatcatg ttcatacctc ttatcttcct cccacagaga tcctattttt ggcaatcaaa     1620

tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta     1680

ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag     1740

agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc     1800

tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg     1860

aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt     1920

tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga     1980

ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag     2040

cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt     2100

gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct     2160

tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac     2220

acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc     2280

ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag     2340

gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa     2400

agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa     2460

agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg     2520

acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt     2580

aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     2640

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     2700

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     2760

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     2820

tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac     2880

ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc     2940

ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac     3000

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     3060

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     3120

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     3180

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     3240

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     3300

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     3360

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     3420

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     3480

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     3540

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     3600

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     3660

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     3720

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     3780

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     3840

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     3900

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     3960

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     4020

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     4080

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     4140

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     4200

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     4260

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     4320

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     4380

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     4440

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     4500

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     4560

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     4620

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     4680

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     4740

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     4800

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     4860

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc     4920

ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact     4980

tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc     5040

cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt     5100

acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc     5160

ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt     5220

gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat     5280

tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa     5340

ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc gcaactgttg     5400

ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt     5460

aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca     5520

ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa     5580

aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc     5640

agaacatttc tctatcgata                                                 5660


<210>  17
<211>  5436
<212>  DNA
<213>  Artificial

<220>
<223>  Plasmid GL3-sint425-sph


<220>
<221>  Intron
<222>  (948)..(1373)

<400>  17
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta       60

gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc      120

cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc      180

ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg      240

caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata      300

aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat      360

aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc      420

gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg      480

aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa      540

ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac      600

atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc      660

gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa      720

aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc      780

gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat      840

agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt      900

gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccaggtg agtctatggg      960

acccttgatg ttttctttcc tgtacacata ttgaccaaat cagggtaatt ttgcatttgt     1020

aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact     1080

ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca     1140

ttctaaagaa taacagtgat aatttctggg ttaaggtaat agcaatattt ctgcatataa     1200

atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac     1260

aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc     1320

caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagagatcct     1380

atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac     1440

ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg     1500

tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg     1560

ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat     1620

ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa     1680

gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact     1740

acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt     1800

gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat     1860

caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg     1920

gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac     1980

tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa     2040

ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc     2100

gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt     2160

gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa     2220

gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt     2280

cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc     2340

ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata     2400

agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt     2460

tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt     2520

aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt     2580

taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc     2640

cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc     2700

cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt     2760

ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag     2820

ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca     2880

tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt     2940

tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc     3000

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     3060

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     3120

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     3180

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     3240

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     3300

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     3360

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     3420

tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt     3480

tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga     3540

tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca     3600

tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat     3660

caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg     3720

cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt     3780

agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag     3840

acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc     3900

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag     3960

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca     4020

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa     4080

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga     4140

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata     4200

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca     4260

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg     4320

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg     4380

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg     4440

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag     4500

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac     4560

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca     4620

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag     4680

tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca     4740

gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct     4800

ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt     4860

tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac     4920

gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct     4980

ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt     5040

ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac     5100

aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca     5160

ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca     5220

agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa     5280

aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac     5340

atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag     5400

tgcaagtgca ggtgccagaa catttctcta tcgata                               5436


<210>  18
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  mutant intron (654 C-T)


<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  beta-globin intron 654 C-T mutation

<400>  18
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  19
<211>  850
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(850)
<223>  Wild-type beta-globin intron

<400>  19
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  20
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  intron with two mutations (654 C-T; 657 TA-GT)


<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  beta-globin intron 654 C-T mutation

<220>
<221>  misc_feature
<222>  (657)..(658)
<223>  beta-globin intron 657 TA-GT mutation

<400>  20
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaagtgc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  21
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (669)..(1518)

<400>  21
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa      840

caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact      900

attatactta atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa      960

gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg     1020

tgcttatttg catattcata atctccctac tttattttct tttattttta attgatacat     1080

aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac     1140

caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt     1200

ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata     1260

caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag     1320

gtaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag     1380

gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg     1440

ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct     1500

cttatcttcc tcccacagag atcctatttt tggcaatcaa atcattccgg atactgcgat     1560

tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat     1620

atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct     1680

tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa     1740

aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc     1800

tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag     1860

gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga     1920

taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga     1980

taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  22
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with wild type intron


<220>
<221>  Intron
<222>  (669)..(1518)

<400>  22
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa      840

caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact      900

attatactta atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa      960

gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg     1020

tgcttatttg catattcata atctccctac tttattttct tttattttta attgatacat     1080

aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac     1140

caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt     1200

ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata     1260

caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag     1320

gcaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag     1380

gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg     1440

ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct     1500

cttatcttcc tcccacagag atcctatttt tggcaatcaa atcattccgg atactgcgat     1560

tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat     1620

atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct     1680

tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa     1740

aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc     1800

tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag     1860

gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga     1920

taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga     1980

taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  23
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with double mutant intron (C654 C-T; 657 TA-GT)


<220>
<221>  Intron
<222>  (669)..(1518)

<400>  23
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa      840

caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact      900

attatactta atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa      960

gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg     1020

tgcttatttg catattcata atctccctac tttattttct tttattttta attgatacat     1080

aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac     1140

caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt     1200

ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata     1260

caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag     1320

gtaagtgcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag     1380

gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg     1440

ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct     1500

cttatcttcc tcccacagag atcctatttt tggcaatcaa atcattccgg atactgcgat     1560

tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat     1620

atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct     1680

tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa     1740

aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc     1800

tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag     1860

gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga     1920

taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga     1980

taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  24
<211>  3355
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (1)..(850)

<220>
<221>  Intron
<222>  (1521)..(2370)

<400>  24
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg      900

ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt      960

cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac     1020

ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac     1080

agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta     1140

tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac     1200

agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt     1260

ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg     1320

gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt     1380

aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg     1440

aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc     1500

gtgagattct cgcatgccag gtgagtctat gggacccttg atgttttctt tccccttctt     1560

ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg     1620

gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt     1680

tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc     1740

gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct     1800

gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt     1860

ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt     1920

taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt     1980

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct     2040

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg     2100

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat     2160

ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa     2220

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt     2280

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc     2340

atgttcatac ctcttatctt cctcccacag agatcctatt tttggcaatc aaatcattcc     2400

ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact     2460

cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt     2520

tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc     2580

cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc     2640

ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct     2700

gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc     2760

cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt     2820

tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag     2880

aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga     2940

caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt     3000

catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga     3060

attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc     3120

cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat     3180

gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg     3240

cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag     3300

aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaa          3355


<210>  25
<211>  4219
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (1)..(850)

<220>
<221>  Intron
<222>  (861)..(1710)

<220>
<221>  Intron
<222>  (2385)..(3234)

<400>  25
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag ccatgagctt gtgagtctat gggacccttg atgttttctt tccccttctt      900

ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg      960

gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt     1020

tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc     1080

gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct     1140

gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt     1200

ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt     1260

taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt     1320

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct     1380

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg     1440

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat     1500

ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa     1560

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt     1620

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc     1680

atgttcatac ctcttatctt cctcccacag ccatgcatgg aagacgccaa aaacataaag     1740

aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag     1800

gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag     1860

gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa     1920

cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc     1980

tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt     2040

tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt     2100

tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa     2160

attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc     2220

acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg     2280

gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc     2340

gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccaggtgagt ctatgggacc     2400

cttgatgttt tctttcccct tcttttctat ggttaagttc atgtcatagg aaggggagaa     2460

gtaacagggt acagtttaga atgggaaaca gacgaatgat tgcatcagtg tggaagtctc     2520

aggatcgttt tagtttcttt tatttgctgt tcataacaat tgttttcttt tgtttaattc     2580

ttgctttctt tttttttctt ctccgcaatt tttactatta tacttaatgc cttaacattg     2640

tgtataacaa aaggaaatat ctctgagata cattaagtaa cttaaaaaaa aactttacac     2700

agtctgccta gtacattact atttggaata tatgtgtgct tatttgcata ttcataatct     2760

ccctacttta ttttctttta tttttaattg atacataatc attatacata tttatgggtt     2820

aaagtgtaat gttttaatat gtgtacacat attgaccaaa tcagggtaat tttgcatttg     2880

taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac     2940

tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc     3000

attctaaaga ataacagtga taatttctgg gttaaggtaa tagcaatatt tctgcatata     3060

aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta     3120

caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt     3180

ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagagatcc     3240

tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca     3300

cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat     3360

gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc     3420

gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga     3480

tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga     3540

agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac     3600

tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt     3660

tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa     3720

tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc     3780

ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag acatagctta     3840

ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa     3900

aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt     3960

cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt     4020

tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca     4080

agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg     4140

tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg     4200

cggaaagatc gccgtgtaa                                                  4219


<210>  26
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T) at alternative 
       location A


<220>
<221>  Intron
<222>  (394)..(1243)

<400>  26
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggtgagtc tatgggaccc ttgatgtttt      420

ctttcccctt cttttctatg gttaagttca tgtcatagga aggggagaag taacagggta      480

cagtttagaa tgggaaacag acgaatgatt gcatcagtgt ggaagtctca ggatcgtttt      540

agtttctttt atttgctgtt cataacaatt gttttctttt gtttaattct tgctttcttt      600

ttttttcttc tccgcaattt ttactattat acttaatgcc ttaacattgt gtataacaaa      660

aggaaatatc tctgagatac attaagtaac ttaaaaaaaa actttacaca gtctgcctag      720

tacattacta tttggaatat atgtgtgctt atttgcatat tcataatctc cctactttat      780

tttcttttat ttttaattga tacataatca ttatacatat ttatgggtta aagtgtaatg      840

ttttaatatg tgtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa      900

aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc      960

tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa     1020

taacagtgat aatttctggg ttaaggtaat agcaatattt ctgcatataa atatttctgc     1080

atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta     1140

ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc     1200

ccttttgcta atcatgttca tacctcttat cttcctccca caggggttgc aaaaaatttt     1260

gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga     1320

ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa     1380

tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa     1440

ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt     1500

gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat     1560

tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat     1620

atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct     1680

tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa     1740

aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc     1800

tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag     1860

gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga     1920

taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga     1980

taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  27
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T) at alternative 
       location B


<220>
<221>  Intron
<222>  (1161)..(2010)

<400>  27
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt      720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt      780

cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac      840

aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg      900

attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct      960

aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat     1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc     1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa     1140

acgctgggcg ttaatcaaag gtgagtctat gggacccttg atgttttctt tccccttctt     1200

ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacag tttagaatgg     1260

gaaacagacg aatgattgca tcagtgtgga agtctcagga tcgttttagt ttcttttatt     1320

tgctgttcat aacaattgtt ttcttttgtt taattcttgc tttctttttt tttcttctcc     1380

gcaattttta ctattatact taatgcctta acattgtgta taacaaaagg aaatatctct     1440

gagatacatt aagtaactta aaaaaaaact ttacacagtc tgcctagtac attactattt     1500

ggaatatatg tgtgcttatt tgcatattca taatctccct actttatttt cttttatttt     1560

taattgatac ataatcatta tacatattta tgggttaaag tgtaatgttt taatatgtgt     1620

acacatattg accaaatcag ggtaattttg catttgtaat tttaaaaaat gctttcttct     1680

tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg     1740

gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat     1800

ttctgggtta aggtaatagc aatatttctg catataaata tttctgcata taaattgtaa     1860

ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt     1920

attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc     1980

atgttcatac ctcttatctt cctcccacag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  28
<211>  2503
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T) at alternative 
       location C


<220>
<221>  Intron
<222>  (1412)..(2261)

<400>  28
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt      720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt      780

cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac      840

aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg      900

attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct      960

aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat     1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc     1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa     1140

acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt     1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct     1260

ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct     1320

ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa     1380

caccccaaca tcttcgacgc aggtgtcgca ggtgagtcta tgggaccctt gatgttttct     1440

ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca     1500

gtttagaatg ggaaacagac gaatgattgc atcagtgtgg aagtctcagg atcgttttag     1560

tttcttttat ttgctgttca taacaattgt tttcttttgt ttaattcttg ctttcttttt     1620

ttttcttctc cgcaattttt actattatac ttaatgcctt aacattgtgt ataacaaaag     1680

gaaatatctc tgagatacat taagtaactt aaaaaaaaac tttacacagt ctgcctagta     1740

cattactatt tggaatatat gtgtgcttat ttgcatattc ataatctccc tactttattt     1800

tcttttattt ttaattgata cataatcatt atacatattt atgggttaaa gtgtaatgtt     1860

ttaatatgtg tacacatatt gaccaaatca gggtaatttt gcatttgtaa ttttaaaaaa     1920

tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt ccctaatctc     1980

tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt ctaaagaata     2040

acagtgataa tttctgggtt aaggtaatag caatatttct gcatataaat atttctgcat     2100

ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa tccagctacc     2160

attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca agctaggccc     2220

ttttgctaat catgttcata cctcttatct tcctcccaca ggtcttcccg acgatgacgc     2280

cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga     2340

gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt     2400

gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga     2460

gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa                       2503


<210>  29
<211>  2505
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T) upstream of 
       translation site


<220>
<221>  Intron
<222>  (1)..(850)

<400>  29
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg      900

ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt      960

cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac     1020

ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac     1080

agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta     1140

tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac     1200

agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt     1260

ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg     1320

gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt     1380

aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg     1440

aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc     1500

gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg     1560

attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg     1620

atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc     1680

cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc     1740

aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc     1800

gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc     1860

aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat     1920

gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg     1980

gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg     2040

attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga     2100

tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac     2160

cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc     2220

atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac     2280

gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa     2340

gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt     2400

gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga     2460

gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaa                     2505


<210>  30
<211>  3353
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with two mutant introns (654 C-T)


<220>
<221>  Intron
<222>  (669)..(1518)

<220>
<221>  Intron
<222>  (1519)..(2368)

<400>  30
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa      840

caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact      900

attatactta atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa      960

gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg     1020

tgcttatttg catattcata atctccctac tttattttct tttattttta attgatacat     1080

aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac     1140

caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt     1200

ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata     1260

caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag     1320

gtaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag     1380

gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg     1440

ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct     1500

cttatcttcc tcccacaggt gagtctatgg gacccttgat gttttctttc cccttctttt     1560

ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga     1620

aacagacgaa tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg     1680

ctgttcataa caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc     1740

aatttttact attatactta atgccttaac attgtgtata acaaaaggaa atatctctga     1800

gatacattaa gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg     1860

aatatatgtg tgcttatttg catattcata atctccctac tttattttct tttattttta     1920

attgatacat aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac     1980

acatattgac caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt     2040

taatatactt ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc     2100

aataatgata caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt     2160

ctgggttaag gtaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact     2220

gatgtaagag gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat     2280

tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat     2340

gttcatacct cttatcttcc tcccacagag atcctatttt tggcaatcaa atcattccgg     2400

atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg     2460

gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc     2520

tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct     2580

tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt     2640

ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc     2700

caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg     2760

agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg     2820

tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag     2880

gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca     2940

aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca     3000

tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat     3060

tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg     3120

acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga     3180

cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg     3240

gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa     3300

aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa            3353


<210>  31
<211>  3353
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with two mutant introns (654 C-T)


<220>
<221>  Intron
<222>  (669)..(1518)

<220>
<221>  Intron
<222>  (2262)..(3111)

<400>  31
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttt cttttatttg ctgttcataa      840

caattgtttt cttttgttta attcttgctt tctttttttt tcttctccgc aatttttact      900

attatactta atgccttaac attgtgtata acaaaaggaa atatctctga gatacattaa      960

gtaacttaaa aaaaaacttt acacagtctg cctagtacat tactatttgg aatatatgtg     1020

tgcttatttg catattcata atctccctac tttattttct tttattttta attgatacat     1080

aatcattata catatttatg ggttaaagtg taatgtttta atatgtgtac acatattgac     1140

caaatcaggg taattttgca tttgtaattt taaaaaatgc tttcttcttt taatatactt     1200

ttttgtttat cttatttcta atactttccc taatctcttt ctttcagggc aataatgata     1260

caatgtatca tgcctctttg caccattcta aagaataaca gtgataattt ctgggttaag     1320

gtaatagcaa tatttctgca tataaatatt tctgcatata aattgtaact gatgtaagag     1380

gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcttttat tttatggttg     1440

ggataaggct ggattattct gagtccaagc taggcccttt tgctaatcat gttcatacct     1500

cttatcttcc tcccacagag atcctatttt tggcaatcaa atcattccgg atactgcgat     1560

tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat     1620

atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct     1680

tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa     1740

aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc     1800

tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag     1860

gcaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga     1920

taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga     1980

taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat     2040

tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg     2100

gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg     2160

cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat     2220

cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtgagtcta tgggaccctt     2280

gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag gggagaagta     2340

acagggtaca gtttagaatg ggaaacagac gaatgattgc atcagtgtgg aagtctcagg     2400

atcgttttag tttcttttat ttgctgttca taacaattgt tttcttttgt ttaattcttg     2460

ctttcttttt ttttcttctc cgcaattttt actattatac ttaatgcctt aacattgtgt     2520

ataacaaaag gaaatatctc tgagatacat taagtaactt aaaaaaaaac tttacacagt     2580

ctgcctagta cattactatt tggaatatat gtgtgcttat ttgcatattc ataatctccc     2640

tactttattt tcttttattt ttaattgata cataatcatt atacatattt atgggttaaa     2700

gtgtaatgtt ttaatatgtg tacacatatt gaccaaatca gggtaatttt gcatttgtaa     2760

ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt     2820

ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt     2880

ctaaagaata acagtgataa tttctgggtt aaggtaatag caatatttct gcatataaat     2940

atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa     3000

tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca     3060

agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca ggtcttcccg     3120

acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga     3180

cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg     3240

gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa     3300

aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taa            3353


<210>  32
<211>  2303
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (669)..(1318)

<400>  32
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttg tgcttatttg catattcata      840

atctccctac tttattttct tttattttta attgatacat aatcattata catatttatg      900

ggttaaagtg taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca      960

tttgtaattt taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta     1020

atactttccc taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg     1080

caccattcta aagaataaca gtgataattt ctgggttaag gtaatagcaa tatttctgca     1140

tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca     1200

gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct     1260

gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagag     1320

atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc     1380

atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct     1440

taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa     1500

gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat     1560

acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg     1620

gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg     1680

agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta     1740

aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg     1800

ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca     1860

atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag     1920

cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt     1980

acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca     2040

tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg     2100

ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca     2160

gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga     2220

aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga     2280

agggcggaaa gatcgccgtg taa                                             2303


<210>  33
<211>  2303
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with double mutant intron (654 C-T; 657 TA-GT)


<220>
<221>  Intron
<222>  (669)..(1318)

<400>  33
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa      720

gttcatgtca taggaagggg agaagtaaca gggtacagtt tagaatggga aacagacgaa      780

tgattgcatc agtgtggaag tctcaggatc gttttagttg tgcttatttg catattcata      840

atctccctac tttattttct tttattttta attgatacat aatcattata catatttatg      900

ggttaaagtg taatgtttta atatgtgtac acatattgac caaatcaggg taattttgca      960

tttgtaattt taaaaaatgc tttcttcttt taatatactt ttttgtttat cttatttcta     1020

atactttccc taatctcttt ctttcagggc aataatgata caatgtatca tgcctctttg     1080

caccattcta aagaataaca gtgataattt ctgggttaag gtaagtgcaa tatttctgca     1140

tataaatatt tctgcatata aattgtaact gatgtaagag gtttcatatt gctaatagca     1200

gctacaatcc agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct     1260

gagtccaagc taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagag     1320

atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc     1380

atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct     1440

taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa     1500

gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat     1560

acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg     1620

gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg     1680

agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta     1740

aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg     1800

ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca     1860

atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag     1920

cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt     1980

acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca     2040

tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg     2100

ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca     2160

gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga     2220

aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga     2280

agggcggaaa gatcgccgtg taa                                             2303


<210>  34
<211>  2079
<212>  DNA
<213>  Artificial

<220>
<223>  luciferase cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (669)..(1094)

<400>  34
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccaggt gagtctatgg gacccttgat gttttctttc ctgtacacat attgaccaaa      720

tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt      780

gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat      840

gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggtaa      900

tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt      960

catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat     1020

aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta     1080

tcttcctccc acagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta     1140

agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt     1200

ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag     1260

gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc     1320

actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc     1380

ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa     1440

ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa     1500

ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc     1560

gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg     1620

tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta     1680

cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg     1740

aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg     1800

ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt     1860

gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc     1920

gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt     1980

gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc     2040

ctcataaagg ccaagaaggg cggaaagatc gccgtgtaa                            2079


<210>  35
<211>  7449
<212>  DNA
<213>  Artificial

<220>
<223>  plasmid TRCBA with alpha antitrypsin cDNA and mutant intron (654 
       C-T)


<220>
<221>  Intron
<222>  (2866)..(3715)
<223>  Mutant beta-globin intron (654C-T)

<400>  35
gggggggggg gggggggttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg       60

ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag      120

cgcgcagaga gggagtggcc aactccatca ctaggggttc ctagatcttc aatattggcc      180

attagccata ttattcattg gttatatagc ataaatcaat attggatatt ggccattgca      240

tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc      300

atgttggcat tgattattga ctagttatta atagtaatca attacggggt cattagttca      360

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc      420

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat      480

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt      540

acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc      600

cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta      660

cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc      720

catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc      780

agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg      840

gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa      900

gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg      960

ggcgggagtc gctgcgacgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc     1020

gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt     1080

ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc     1140

gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt     1200

gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg     1260

agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg     1320

ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg     1380

gtgtgtgcgt gggggggtga gcagggggta tgggcgcggc ggtcgggctg taaccccccc     1440

ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg     1500

gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc     1560

ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc     1620

gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag     1680

agggcgcagg gacttacttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc     1740

cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc     1800

ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc     1860

tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg     1920

cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta     1980

cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattcgat     2040

atcaagcttg gggattttca ggcaccacca ctgacctggg acagtgaatc gacaatgccg     2100

tcttctgtct cgtggggcat cctcctgctg gcaggcctgt gctgcctggt ccctgtctcc     2160

ctggctgagg atccccaggg agatgctgcc cagaagacag atacatccca ccatgatcag     2220

gatcacccaa ccttcaacaa gatcaccccc aacctggctg agttcgcctt cagcctatac     2280

cgccagctgg cacaccagtc caacagcacc aatatcttct tctccccagt gagcatcgct     2340

acagcctttg caatgctctc cctggggacc aaggctgaca ctcacgatga aatcctggag     2400

ggcctgaatt tcaacctcac ggagattccg gaggctcaga gccatgaagg ctgccaggaa     2460

ctcctccgta ccctcaacca gccagacagc cagctccagc tgaccaccgg caatggcctg     2520

tgcctcagcg agggcctgaa gcaagtggat aagtttttgg aggatgttaa aaagttgtac     2580

cactcataag ccttcactgt caacttcggg gacaccgaag aggccaagaa acagatcaac     2640

gattacgttg agaagggtac tcaagggaaa atggtggatg tggtcaagga gcttgacaga     2700

gacacagttt ttgctctggt gaattacatc ttctttaaag gcaaatggga gagacccttt     2760

gaagtcaagg acaccgagga agaggacttc cacgtggacc aggtgaccac cgtgaaggtg     2820

cctatgatga agcgtttagt catgtttaac atccagcact gtaaggtgag tctatgggac     2880

ccttgatgtt ttctttcccc ttcttttcta tggttaagtt catgtcatag gaaggggaga     2940

agtaacaggg tacagtttag aatgggaaac agacgaatga ttgcatcagt gtggaagtct     3000

caggatcgtt ttagtttctt ttatttgctg ttcataacaa ttgttttctt ttgtttaatt     3060

cttgctttct ttttttttct tctccgcaat ttttactatt atacttaatg ccttaacatt     3120

gtgtataaca aaaggaaata tctctgagat acattaagta acttaaaaaa aaactttaca     3180

cagtctgcct agtacattac tatttggaat atatgtgtgc ttatttgcat attcataatc     3240

tccctacttt attttctttt atttttaatt gatacataat cattatacat atttatgggt     3300

taaagtgtaa tgttttaata tgtgtacaca tattgaccaa atcagggtaa ttttgcattt     3360

gtaattttaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata     3420

ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac     3480

cattctaaag aataacagtg ataatttctg ggttaaggta atagcaatat ttctgcatat     3540

aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct     3600

acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag     3660

tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagaagct     3720

ttccagctgg gtgctgctga tgaaatacct gggcaatgcc accgccatct tcttcctgcc     3780

tgatgagggg aaactacagc acctggaaaa tgaactcacc cacgatatca tcaccaagtt     3840

cctggaaaat gaagacagaa ggtctgccag cttacattta cccaaactgt ccattactgg     3900

aacctatgat ctgaagagcg tcctgggtca actgggcatc actaaggtct tcagcaatgg     3960

ggctgacctc tccgtggtca cagaggaggc acccctgaag ctctccaatg ccgtgcataa     4020

ggctgtgctg accatcgacg agaaagggac tgaagctgct ggggccatgt ttttagaggc     4080

catacccatg tctatccccc ccgaggtcaa ggtcaacaaa ccctttgtct tcttaatgat     4140

tgaacaaaat accaagtctc ccctcttcat gggaaaagtg gtgaatccca cccaaaaata     4200

actgcctctc gctcctcaac ccctcccctc catccctggc cccctccctg gatgacatta     4260

aagaagggtt gagctggtaa cccccccccc ccctgcaggg gccctcgacc cgggcggccg     4320

cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag     4380

tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata     4440

agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg     4500

gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag     4560

gatctaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac     4620

tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag     4680

cgagcgagcg cgcagagagg gagtggccaa cccccccccc cccccccctg cagcctggcg     4740

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga     4800

atggcgcgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag     4860

cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt     4920

tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt     4980

ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg     5040

tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt     5100

taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt     5160

tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca     5220

aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcctgat gcgctatttt     5280

ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc     5340

tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga     5400

cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc     5460

atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata     5520

cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact     5580

tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaatact ttcaaatatg     5640

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt     5700

atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct     5760

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca     5820

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc     5880

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc     5940

cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg     6000

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta     6060

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc     6120

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt     6180

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg     6240

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct     6300

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc     6360

tcggcccttc cggctggctg gtttattgcg gataaatctg gagccggtga gcgtgggtct     6420

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac     6480

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc     6540

tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat     6600

ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg     6660

accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc     6720

aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa     6780

ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag     6840

gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta     6900

ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta     6960

ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag     7020

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg     7080

gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg     7140

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag     7200

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc     7260

cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa     7320

aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     7380

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     7440

gataccgct                                                             7449


<210>  36
<211>  2107
<212>  DNA
<213>  Artificial

<220>
<223>  alpha antitrypsin cDNA with mutant intron (654 C-T)


<220>
<221>  Intron
<222>  (772)..(1621)
<223>  Mutant beta globin intron (654C-T)

<400>  36
atgccgtctt ctgtctcgtg gggcatcctc ctgctggcag gcctgtgctg cctggtccct       60

gtctccctgg ctgaggatcc ccagggagat gctgcccaga agacagatac atcccaccat      120

gatcaggatc acccaacctt caacaagatc acccccaacc tggctgagtt cgccttcagc      180

ctataccgcc agctggcaca ccagtccaac agcaccaata tcttcttctc cccagtgagc      240

atcgctacag cctttgcaat gctctccctg gggaccaagg ctgacactca cgatgaaatc      300

ctggagggcc tgaatttcaa cctcacggag attccggagg ctcagagcca tgaaggctgc      360

caggaactcc tccgtaccct caaccagcca gacagccagc tccagctgac caccggcaat      420

ggcctgtgcc tcagcgaggg cctgaagcaa gtggataagt ttttggagga tgttaaaaag      480

ttgtaccact cataagcctt cactgtcaac ttcggggaca ccgaagaggc caagaaacag      540

atcaacgatt acgttgagaa gggtactcaa gggaaaatgg tggatgtggt caaggagctt      600

gacagagaca cagtttttgc tctggtgaat tacatcttct ttaaaggcaa atgggagaga      660

ccctttgaag tcaaggacac cgaggaagag gacttccacg tggaccaggt gaccaccgtg      720

aaggtgccta tgatgaagcg tttagtcatg tttaacatcc agcactgtaa ggtgagtcta      780

tgggaccctt gatgttttct ttccccttct tttctatggt taagttcatg tcataggaag      840

gggagaagta acagggtaca gtttagaatg ggaaacagac gaatgattgc atcagtgtgg      900

aagtctcagg atcgttttag tttcttttat ttgctgttca taacaattgt tttcttttgt      960

ttaattcttg ctttcttttt ttttcttctc cgcaattttt actattatac ttaatgcctt     1020

aacattgtgt ataacaaaag gaaatatctc tgagatacat taagtaactt aaaaaaaaac     1080

tttacacagt ctgcctagta cattactatt tggaatatat gtgtgcttat ttgcatattc     1140

ataatctccc tactttattt tcttttattt ttaattgata cataatcatt atacatattt     1200

atgggttaaa gtgtaatgtt ttaatatgtg tacacatatt gaccaaatca gggtaatttt     1260

gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt     1320

ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct     1380

ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggtaatag caatatttct     1440

gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata     1500

gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag gctggattat     1560

tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca     1620

gaagctttcc agctgggtgc tgctgatgaa atacctgggc aatgccaccg ccatcttctt     1680

cctgcctgat gaggggaaac tacagcacct ggaaaatgaa ctcacccacg atatcatcac     1740

caagttcctg gaaaatgaag acagaaggtc tgccagctta catttaccca aactgtccat     1800

tactggaacc tatgatctga agagcgtcct gggtcaactg ggcatcacta aggtcttcag     1860

caatggggct gacctctccg tggtcacaga ggaggcaccc ctgaagctct ccaatgccgt     1920

gcataaggct gtgctgacca tcgacgagaa agggactgaa gctgctgggg ccatgttttt     1980

agaggccata cccatgtcta tcccccccga ggtcaaggtc aacaaaccct ttgtcttctt     2040

aatgattgaa caaaatacca agtctcccct cttcatggga aaagtggtga atcccaccca     2100

aaaataa                                                               2107


<210>  37
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  37
gctattacct taacccag                                                     18


<210>  38
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  38
gcacttacct taacccag                                                     18


<210>  39
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 6A mutation in IVS2-654

<400>  39
caagggtccc atagtctc                                                     18


<210>  40
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 564C mutation in IVS2-654

<400>  40
gaaagagatg agggaaag                                                     18


<210>  41
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 564CT mutation in IVS2-654

<400>  41
gaaagagaag agggaaag                                                     18


<210>  42
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 705G mutation in IVS2-705

<400>  42
cctcttacct cagttaca                                                     18


<210>  43
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 841A mutation in IVS2-654

<400>  43
ctgtgggagt aagataag                                                     18


<210>  44
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 657G mutation in IVS2-654

<400>  44
gctcttacct taacccag                                                     18


<210>  45
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for 658T mutation in IVS2-654

<400>  45
gcaattacct taacccag                                                     18


<210>  46
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for IVS2-654

<400>  46
caagggtccc atagactc                                                     18


<210>  47
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for IVS2-654

<400>  47
gaaagagatt agggaaag                                                     18


<210>  48
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for IVS2-654

<400>  48
ctgtgggagg aagataag                                                     18


<210>  49
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  oligo for IVS2-705

<400>  49
cctcttacat cagttaca                                                     18


<210>  50
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 564CT mutation


<220>
<221>  misc_feature
<222>  (564)..(565)
<223>  564CT mutation

<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<400>  50
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctcttctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  51
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 657G mutation


<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<220>
<221>  misc_feature
<222>  (657)..(657)
<223>  657G mutation

<400>  51
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaagagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  52
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 658T mutation


<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<220>
<221>  misc_feature
<222>  (658)..(658)
<223>  658T mutation

<400>  52
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaattgc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  53
<211>  650
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 200 bp deletion


<220>
<221>  misc_feature
<222>  (454)..(454)
<223>  C to T mutation

<400>  53
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt tgtgcttatt tgcatattca taatctccct      180

actttatttt cttttatttt taattgatac ataatcatta tacatattta tgggttaaag      240

tgtaatgttt taatatgtgt acacatattg accaaatcag ggtaattttg catttgtaat      300

tttaaaaaat gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc      360

cctaatctct ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc      420

taaagaataa cagtgataat ttctgggtta aggtaatagc aatatttctg catataaata      480

tttctgcata taaattgtaa ctgatgtaag aggtttcata ttgctaatag cagctacaat      540

ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa      600

gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag                 650


<210>  54
<211>  426
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 425 bp deletion


<220>
<221>  misc_feature
<222>  (230)..(230)
<223>  C to T mutation

<400>  54
gtgagtctat gggacccttg atgttttctt tcctgtacac atattgacca aatcagggta       60

attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct      120

tatttctaat actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg      180

cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggt aatagcaata      240

tttctgcata taaatatttc tgcatataaa ttgtaactga tgtaagaggt ttcatattgc      300

taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg ataaggctgg      360

attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct tatcttcctc      420

ccacag                                                                 426


<210>  55
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 6A mutation


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  6A mutation

<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<400>  55
gtgagactat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  56
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 564C mutation


<220>
<221>  misc_feature
<222>  (564)..(564)
<223>  564C mutation

<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<400>  56
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctcatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  57
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron with 841A mutation


<220>
<221>  misc_feature
<222>  (654)..(654)
<223>  654T mutation

<220>
<221>  misc_feature
<222>  (841)..(841)
<223>  841A mutation

<400>  57
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggtaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

actcccacag                                                             850


<210>  58
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron


<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  58
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  59
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 564 CT mutation


<220>
<221>  misc_feature
<222>  (564)..(565)
<223>  564CT mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  59
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctcttctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  60
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 657G mutation


<220>
<221>  misc_feature
<222>  (657)..(657)
<223>  657G mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  60
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaagagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  61
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 658T mutation


<220>
<221>  misc_feature
<222>  (658)..(658)
<223>  658T mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  61
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaattgc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  62
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 657GT mutation


<220>
<221>  misc_feature
<222>  (657)..(658)
<223>  657GT mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  62
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaagtgc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  63
<211>  650
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 200 bp deletion


<220>
<221>  misc_feature
<222>  (505)..(505)
<223>  T to G mutation

<400>  63
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt tgtgcttatt tgcatattca taatctccct      180

actttatttt cttttatttt taattgatac ataatcatta tacatattta tgggttaaag      240

tgtaatgttt taatatgtgt acacatattg accaaatcag ggtaattttg catttgtaat      300

tttaaaaaat gctttcttct tttaatatac ttttttgttt atcttatttc taatactttc      360

cctaatctct ttctttcagg gcaataatga tacaatgtat catgcctctt tgcaccattc      420

taaagaataa cagtgataat ttctgggtta aggcaatagc aatatttctg catataaata      480

tttctgcata taaattgtaa ctgaggtaag aggtttcata ttgctaatag cagctacaat      540

ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt ctgagtccaa      600

gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag                 650


<210>  64
<211>  426
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 425 bp deletion


<220>
<221>  misc_feature
<222>  (281)..(281)
<223>  T to G mutation

<400>  64
gtgagtctat gggacccttg atgttttctt tcctgtacac atattgacca aatcagggta       60

attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt ttgtttatct      120

tatttctaat actttcccta atctctttct ttcagggcaa taatgataca atgtatcatg      180

cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc aatagcaata      240

tttctgcata taaatatttc tgcatataaa ttgtaactga ggtaagaggt ttcatattgc      300

taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg ataaggctgg      360

attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct tatcttcctc      420

ccacag                                                                 426


<210>  65
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 6A mutation


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  6A mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  65
gtgagactat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  66
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 564C mutation


<220>
<221>  misc_feature
<222>  (564)..(564)
<223>  564C mutation

<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<400>  66
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctcatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

cctcccacag                                                             850


<210>  67
<211>  850
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-705 intron with 841A mutation


<220>
<221>  misc_feature
<222>  (705)..(705)
<223>  705G mutation

<220>
<221>  misc_feature
<222>  (841)..(841)
<223>  841A mutation

<400>  67
gtgagtctat gggacccttg atgttttctt tccccttctt ttctatggtt aagttcatgt       60

cataggaagg ggagaagtaa cagggtacag tttagaatgg gaaacagacg aatgattgca      120

tcagtgtgga agtctcagga tcgttttagt ttcttttatt tgctgttcat aacaattgtt      180

ttcttttgtt taattcttgc tttctttttt tttcttctcc gcaattttta ctattatact      240

taatgcctta acattgtgta taacaaaagg aaatatctct gagatacatt aagtaactta      300

aaaaaaaact ttacacagtc tgcctagtac attactattt ggaatatatg tgtgcttatt      360

tgcatattca taatctccct actttatttt cttttatttt taattgatac ataatcatta      420

tacatattta tgggttaaag tgtaatgttt taatatgtgt acacatattg accaaatcag      480

ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt      540

atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat      600

catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc      660

aatatttctg catataaata tttctgcata taaattgtaa ctgaggtaag aggtttcata      720

ttgctaatag cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg      780

ctggattatt ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt      840

actcccacag                                                             850


<210>  68
<211>  196
<212>  DNA
<213>  Artificial

<220>
<223>  IVS2-654 intron 197 bp

<400>  68
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct cttctctttc tttcaggtga ttgactgact gggttaaggt aatagcgccg      120

ttgaaaacct cagccgtata gtccaagcta ggcccttttg ctaatcatgt tcatacctct      180

tatcttcctc ccacag                                                      196


<210>  69
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  IVS-654 intron 247 bp

<400>  69
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgggttaagg taatagcaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcttcct      240

cccacag                                                                247


<210>  70
<211>  14667
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(14667)
<223>  CFTR gene exon 19

<220>
<221>  misc_feature
<222>  (12191)..(12191)
<223>  3849 + 10 kb C-to-T mutation site

<400>  70
gtgagatttg aacactgctt gctttgttag actgtgttca gtaagtgaat cccagtagcc       60

tgaagcaatg tgttagcaga atctatttgt aacattatta ttgtacagta gaatcaatat      120

taaacacaca tgttttatta tatggagtca ttatttttaa tatgaaattt aatttgcaga      180

gtcctgaacc tatataatgg gtttatttta aatgtgattg tacttgcaga atatctaatt      240

aattgctagg ttaataacta aagaagccat taaataaatc aaaattgtaa catgttttag      300

atttcccatc ttgaaaatgt cttccaaaaa tatcttattg ctgactccat ctattgtctt      360

aaattttatc taagttccat tctgccaaac aagtgatact ttttttctag cttttttcag      420

tttgtttgtt ttgtttttct ttgaagtttt aattcagaca tagattattt tttcccagtt      480

atttactata tttattaagc atgagtaatt gacattattt tgaaatcctt cttatggatc      540

ccagcactgg gctgaacaca tagaaggaac ttaatatata ctgatttctg gaattgattc      600

ttggagacag ggatggtcat tatccatata cttcaggctc cataaacata tttcttaatt      660

gccttcaaat ccctattctg gactgctcta taaatctaga caagagtatt atatattttg      720

attgatattt tttagataaa ataaaaggga gctgaaaact gaattgcaaa ctgaatttta      780

aaactttatc tctctgtggt taattgcaaa cacagataca aaaatataga gagagataca      840

gttagtaaag atgttaggtc accgttacta acactgacat agaaacagtt ttgctcatga      900

gtttcagaat atatgagttt gattttgccc atggatttta gaatatttga taaacattta      960

atgcattgta caaattctgt gaaaacatat atataggatg tgcgaaaagt ccctgtgtat     1020

catgtgaaat ggcttaaaac agaacaccat aggtattcat atcagtgaat accataggta     1080

gctgaaagtg ttttttcctg gggtcgccaa gatgaatgcc aaaagtgata tcattattat     1140

aaacaatagc cagaataggt tggtataaac ctggtagaaa gccttgataa attgactttc     1200

tctcctcctg acatcctgcc acccctttgc tttgctgatg ctcatttgtc cactaaatta     1260

aactcaagca agccctagta aagtaataga atttgtggag tcctcattag tataggaagt     1320

ttccctgatg tgagattagt aattagagat gtagcaaaat gagaaagaag taatatgctt     1380

agatatttca ttttctctga acctgtatat acaaaatagg ccatgcgtgt tcagtaacta     1440

ttcactgcaa ggcactctct aggtactttg ggggaattgg aaattactca cataaggcta     1500

tggattgtgc catttgtcaa aagacaaaat gacaacaaat ttagtttaaa gacctcagtc     1560

agctttattt tctattctag atttggacag tccttcattt cacaaattgg agtaagtgtt     1620

ccaataagtt gagcaaagga gcttggcttt atagacccaa aaaaagggcc aaaggaagca     1680

gaaacaaaga acaataagag aattggtcat ttcaaagtta cttttcttga aaggtgggga     1740

caaggagaca gaataataga aaagtcactg attggttaac attggattaa gaattaaaac     1800

agaggaaact ttaagattga agtttgaaac tgacttgttt gggaaatcag gctgtcttct     1860

ttcttgattt cttagaaggc cggataacaa ctgagttttg ctttggtgaa catgggtgac     1920

tccattttta cttttagtct ggtctgttga ggcctcgtga gagagcttaa tctaaaacaa     1980

tgacttccta taatttttgt ttgacacatc caaagaggga ctctaatatt tattgagagc     2040

ttatcatatc ttaagtactg tttaaacact tttatttgct attacatttg atcttattat     2100

aactctaaag gcagaaatga ttgcttttat tttccacaat ggaggaaact gaggttcaat     2160

taagtgagta aggaagcagg gatcttaaac ccagatacca ttgctcctct ttaaaggtgg     2220

aagaacagaa aacatggggc aggggaagag agaaagtttc tgtcccagga catgataatc     2280

taaaagggaa aacgtaagat ccactgaaac ctgaggcaga tttattgtgg caataacaaa     2340

gcttaagttt cacagacctt catttgcctg agccaacttt gaaggccatg tatctaattt     2400

tgtttttata attctataat ctttattctt gaaaagagcc ctccctccaa atttacaagc     2460

tttgggcccc caaaatcctt gaaatgccct tgaataagag atatccaggt aaatgctatg     2520

ggaattcaga ggaggaagca gttagtatca gttggcggag agttaggcta ttaagagaag     2580

gttttatata ggaagtggca tttagaatga agctttgaga actgagctgt gtatttgaac     2640

aagtaaaggt ggtgttgcag aattttgctc cttagttcta ttaaaaaccc gggttcttgt     2700

cacatgatcc ggaaaattta ggcacacaga tacattgaag catgagtaga gcaggatttt     2760

attgggcaaa aaggaaaaaa agaaaactca gcaaatcgag atggagtctt gctcacagat     2820

tgaatcccag gccaccacaa aggaactgaa gagatcgggc ttctcccctg cataaggtgc     2880

aaattcccca tggctccacc cacttcccct tagtgtgcat gtggggctcc agtccacggt     2940

gggcatgccc agacaagcct tgggcaggtt ccctcatctg tgcaaaagca tctgatgtaa     3000

acacttgagg ggtggttcgg agattctctg ggaccctttt attttcttat ctgcctaggc     3060

atttggctgt ctcagtgggt gggaaagggt gctccaggca aagggcataa catgaggcaa     3120

agggcatgca cagaaaacag tgactggttc agtcaggttg ggggatgcca aaggaagtaa     3180

tgggagacaa gattggagca agatagataa gagattgtgg attttttttc ttttttatct     3240

atataaatac agagacaggg tctcactatg ttgcccaggc tggtctcaaa ctcctggcct     3300

caagtgatcc tcccacctca tcctcccaaa gtgctaggat tacaggcatg aggcactgtg     3360

cccaacctcc aattttggat tttgagagct aaagcaatat agtcgaaaac tcagataatc     3420

caggtagatt ttgctattag gtgctatttg gttcctggta cagagctaaa acccttggaa     3480

tttcctaagt gataagagct acaggagcat cttttgttat atgtttcccc ccctagttcc     3540

tgaaatagct ctagagaaat acaggtgaat aacatccttt gttattcata tcaagcccct     3600

atcaaccata ccccagtttc tatttatgaa gtggcttttg ggaagtccct aaagacagga     3660

gtggggaaag gctggttgtc agggggatgg gttgaaactt tcatcttccc cccttgacct     3720

ccagggaggg atgagtggct gaaaattgtg taaaatcaac aatggccagt gatttaatca     3780

accatgccta tgtaatgaag ccacccgata agccttaact ggaacttttt ggagagcctc     3840

caggctggtg aagacattga ggtgctcaga aggtggtatt ccagagagag cacagaatct     3900

ctgttcccct tcccacattc attttgctat gcatctctcc catctggctg ttcttgagag     3960

gtatccgttt ataataaact ggtaacctag taagtaaact gttaccctga gttctgtgag     4020

ccattctagc aaattatcaa acctaaagag ttcatggata cgtgcaattt acagatgcac     4080

agtcagaagc acagatgaca atctgggctt gccattggca tttgaagtgt gttgggaggc     4140

agtcttacag gaatgagccc ttatcctgtg gggtctatgc taataacaga cagttgtcag     4200

cattgcttgg tgtcgaaaac ccacattgtt ggtgtcagaa gtattgtcag taggataggg     4260

aaaacagttt gttttctttt tttagtggtc tttggtcatc tttaagagca gggcttctca     4320

aagtgtggtc cttgaaccag catcacctgt accacgtaag aacttatgag aaatgttcat     4380

tcttgggccc caacaaagaa ttaaaaattc tgagggtgtg aacggggtct gagtttcagc     4440

acaacttccc gaccatgctg atgcattctt gcccaagcat gaaagccctc ccttgtttaa     4500

gaaggccatt agggccgggt gtggtggctc atgcttgtaa tcgagcactt tgagaggaca     4560

tagtgggagg atcacttgag ccctggagtt ctagacaagc ctgggcaaca tggcaaaatg     4620

ctgtctccac aaaaatcaca aaaattaggt gggcgtgtgt tgtgtgccta taggcccagc     4680

tacttaggag actgaggcag gaggatcgct tgagcccagg agattaaggc tgcagcgagc     4740

tgtgatggca ccactacagc ctggatgaca gagtgagaca ctgtctcaaa aaaaaaaaag     4800

aaaaagaaaa agaaaaaaga aaggaaaatg aaaaagaacg ccattaggta taaaggagca     4860

atggtaaaag accagttgca aaaggttagg gaatgggtgg ttactgaaat aagaagctat     4920

gtagaacact agtgttggtg gcaggaagta gaaagcaaga gcactgctct gtgggggatg     4980

gtcatagcaa atgcaatatg gaggcatttg cctctgcact gaggagaaaa ctatcttttc     5040

caagatagga ggaaaggaga taagtggaat taaagagaac ctttgagcac agagttggga     5100

aactgaaggt atttgtgttg tgctccctca atcttttaat tcaactataa gctaaaccca     5160

tgaaacttga gtagtttcag ttatctgact tttttcttct cttttgatac agtgttggct     5220

attctgggtc ttttgcctct ctttatgtac ttaagaatca gtttgccaat gtatgcaaaa     5280

taactggctg ggattttgat tgtgattggc ttgaatctat agatggagtt gggaaggact     5340

gacatcttga caatgttgaa gcttcctatt catcattatg aaatatttct ccatttgttt     5400

gattctttga tttcttttat cagaatttag ttttcctcat atagtctttt aaaatatttt     5460

gttatatttt gttcaagtat tttgtttttg aggaatgcca atgtaaatgg tattgtgatt     5520

ttaatttcaa attccaattt ttcattgctg ttatatagga aaatgatttt ttttgcatgt     5580

tagccttata tctttcaact ttgctataat caattattga tagtttcaag gattttttgg     5640

tcaattattt tgaatcttct acatagatta tcatcatctg aacttagttt tatttcttcc     5700

ttcccaatct gtataccttt atctcctttt cttatttcat tagctaggac ttccagtatg     5760

atgttgaaag tagtggtgag aggggatatc ttggtcttgt tcttgatctt agtgggaaaa     5820

cttcaagttt cttatcatta agtatgattt tagctggagg gtttttgtag aagttttttt     5880

tttttaagtt gaagaagtct ccttctattt ttagtttgct gatttttaaa aagaatcagg     5940

aatgggtgtt aaattttgtg aaatgctttt ctgcaactat tgatttgagc actttatttt     6000

tcttctttgg cttgttgatg tgaagtacat taattgattt ttgaatgctg aatcaacctt     6060

ttgtacctga gattaatccc gtttggttgt ggtatataat tatttgtata catgttgagt     6120

tcgatttgct aatacttttt gagaattttt gcattggtgt tcatgaaaaa atattggtgt     6180

gtagtttttt gtgacatctt tatctgctta tggttttaag gtaatgctgg cctcatagca     6240

tgagttaggg agtatttcct ctacttttac atttgagaag agattgcaga gaattagtaa     6300

aattcctact ttaaatattt tgtggaattc accagtgaac ccatctggac ctggtgcttt     6360

ctgttttgga aggtcattaa ttattttaaa atagatatag gcctattcag attacctatt     6420

ttttctcatg cgagttttag cagattgtct ttcaaggaat tggtctattt catttaggtt     6480

atcaaatatg tcaacgtaga gttattcata gtattctttt attatccttt taatgtgcaa     6540

gggatctgta gtgatgtccc cttttttgtt ttattgatat tagcaatttg tgtcacatct     6600

tttattttgc tttgttagcc aggctagaga tatctctatt tttgatgttt ttgatgaacc     6660

aactttttgt tttattgatt ttctctgttg atttcgtgat ttcaatttca tgatttttaa     6720

attatgctta catttgattt aatttgatct tcttttgcta gttatccaag gtggaagctt     6780

atattgttaa gatccttttg cattcttatg cattcaatga tgtaaatttc cctctaagca     6840

ctgctttttc tgcatctcac aaatattcat gagttgtatt ttcatgttca tttagtttga     6900

aatattttta aatttctctt gatatttctc ttttgaccca tgtgttactt agaagtgtgt     6960

tgtttaatca ccatttttaa aaattttcta gctatctttc tgttattgat ttctagttta     7020

attccattgt ggtctgagag catatattgt ataattttaa tttttataaa atttgttaag     7080

gtgtgattta tggcccagaa tgtggtctat cttggtgaat gttccatgta agctttggaa     7140

gactgtgtat tctgctatat ttgaatgagg tagtctatag acatcaatta tgtccagttg     7200

attgatggtg ctgttgaatt caactatgtc cttactgatt ttccacctgc tagatctgtc     7260

cattctttgc agagggacac tgaagtctcc aactctagta gtgaatattc tatttcttgt     7320

tacagtttta tcaacttctg cttcatgtct tttgatgctt tgttgctaga aacatacaca     7380

tgaagaattg gtatgtcttt tggagcatga cccatttatc ctcatataat gcccctcatt     7440

atttcctcgc cctgatgtct gttctctctg aaagaaatat agcctctcca ggtctctttt     7500

ggttggtgtt aaaatgactt aactttcttt atccccctta cttttagttt atatgtggtt     7560

ttaaatttaa agtgggtttc ttgtagacag caaatagttc agagttgttt ttcgatccac     7620

tttgacaatc tttgtctttt aattggtata tttggactat tgatatttta agtgattatt     7680

gatatagtta gataaacatc tactatattt attactgttt tctgtctgtt acactacttg     7740

ttctttgttt atatttttat tgtctactct ttttctttcc attgtggttt taatcgagca     7800

ttttatatgt ttccattttc ttttcttagc atagtaattc ttctttaaaa aaacattttt     7860

tagtggttgc ccctagagtt tgcaatatac atttacaact aatctaagtc cattttcaaa     7920

taatactaaa taatttcatg tgtagtgcaa gtacctttta ataataaaac actcccagtt     7980

ccaccttcca gtctcttgta ttatagctat aatttagttc acttacatat atgggtatac     8040

ctaagtatat acattatcat atttatgatt gaatatattg atgaaattat tttgaaaaaa     8100

ctgttatcgt taaatcaatt aagagtaaga aaaatagttc taattttatt ataaaatgaa     8160

ataccttcat ttattcattc tctaatacac tttctttctt tatgtagatc caagtttctg     8220

acctgtataa ttttcctttt ctctcttcag cttctttgaa catttcttac cagccagacc     8280

tactgacaac aattttcccc aatttttgtt tgtctgatag agactttatt tcttcttgac     8340

ttttgaagaa taattccaca gggcacagaa ctctagattg gtgatttctt cccctcaaac     8400

ccttaaatat ttcattccac tgccttcttg cttgcattgt ttctgagaag ttagatataa     8460

ttcttatctt tgcctttcta taggtaagat gttttttcct ctggcttcta tcaagatttt     8520

ttctttatga acatgatatg cctttctttt tgaacatgat atgcctttct ttttgaacat     8580

gatatgcctt tgtgtcggat tttttttggc attattctgc ttggttttct ctgagtttct     8640

tggatatgtg gtatggtatc tgacactaat ttggaaaaat tctcagtcat tattgcttca     8700

aatatttctt ctgttctttt ttttccttta ttctccttct ggtattccca ttacatgtat     8760

gttacagttt ttgtagtcat cccgctgttt tggatattct gtttttttca gttttttttt     8820

ccttcgcatt tcagtgttgg aagtttctat tgacatattc tcaacctcag agattctttc     8880

ttcagctgtg ttcagtctac caatgagtcc atcaaaggca ttttacattt ttattacaga     8940

atttttgacc tatagaattt cttttgattc catctttgaa tctccatttc tcttctgctt     9000

ttcatctgtt cttgcatgtt gcctactttt tccatgaaaa cctttagctt tttttttttt     9060

tctttttgag gtggagtctc actgttgccc aggctggagt gcagtggtgt gatcttggct     9120

cactgcaacc tctgcctcct gggttcaagt gattctcctc ctcagcctcc caagtagctg     9180

ggattacagg tgcctgccac catgcctgag taatttttgt atttttagta gagatggggt     9240

tttatcatgt tggccaggcg ggtcttgaac tcctaacctc aagtgatctg cccaccttag     9300

cctcccaaat tgctgggatt ataggtgtga gccaccatgc cctgccttta gcatgttaat     9360

catagttgtt ttaaattcct gatctgttaa ttccaacatc cctgtcatat ctgactgtgg     9420

ttctgatgct tgctctgtgt tttcaaatgg tgtttttttt tttttgcctt ttagtaagcc     9480

ttgtaatttt ttattgaaag gtggacatga tgtgctgggt aaaaggaact gtagtaaata     9540

ggcctttagt aatgtactgg taggtgtagc agagggtgag ggaagtattc tgtagtccta     9600

tgattaggtt ttagtctttt agtgagcctg tgcgcctgca gcttggaagc acttgtgaag     9660

tgttttttca ccccttttgg tgggacatag tgactagtgt gagcgggagt tgagtatttc     9720

ccttccccta ggtcagttag gctctgaaaa aaccctgata ggttaggcat ggtaaaatag     9780

tctcttttga gggcaggcat tgttataaga atagaatgct ctggggccag gtgcggtggc     9840

tcacgcctgt aatccccgca ctttgggagg ctaaggcagg tggatcacct gaggtcagga     9900

gttcgagacc agcctggcca acatggtgaa accccgtctc tactaaaaat acaaaaatca     9960

gccaggtgtg gtggcacaca cctataatcc cagctactca ggaggctgag gcaggagaac    10020

tgcttgaacc cagtaagtgg aggttacagt gacccaagat tgtgccactg cagtctagtc    10080

tgggtgacag agcaagactc cgtctcaaaa aaaaaagaat gctctggcat atttgaaaat    10140

ggttactttt cccttttttt ctctgatctt cactgtgaga acctggtaag catcctatag    10200

gcaaaattca taaaagtata gaagtcggcc agtgacttgg acccacttgg aattttcttg    10260

ctctcacatc atgcacactg aatctccagc aatttttcac ttacagttta ggttttccta    10320

ccctactact ggttctctca gaggtttctg cttattggtt tctgttttgt aagttgtgat    10380

tctctgtacc taactgcctg tctcccattt tggggggcag tggtttgccc tgtgacctca    10440

cttctctgac agatctaaga aaagttgttt atttttcagt gtgctctgct ttttacttgt    10500

tacgatgaag ccaaccactt tcagaatttc tacaaaccag atcagaatct ggaagtcctg    10560

tttttttatt ttttttatcc ctttgtttag catgttacct atcttaacac attttaaata    10620

agtgaatgca tagcttatat ctacttctag gttatatgct tccttagaat aggaattgat    10680

tcttaaaatg tcgttctgct cacgcctgta attccagcac tttgggaggc caaggcaggc    10740

ggatcacttg gggtcaggag ttcaagacca gcctggtcaa catggtaaaa ccctgtgcct    10800

gcaaaaaata caaaaattag ctgggcatgg tggtggccat ctgtaatccc agctactagg    10860

gaagctaagg catgagaatc acttgaacct gggaggtgga ggttgcagtg agctgagatc    10920

gcgccactgc actccagcct gggtgacaag agcaaaactc catctcataa ataaataaat    10980

aaataaataa ataaataata aaaataaaaa aataaaataa aacaaaaatt ttattctgag    11040

cagtctctga agaatataaa ttctactgcc ttgcctttag aacttataac agcatctcgc    11100

aaactatcac aagatgctcc aaacatactt cttatgtgct gaattaagaa gtcaactcaa    11160

atttagtata ctagtaatat ttttggatat cccaaaacac tgccagctca gctttaggct    11220

gcccttcttg ggggggaaaa aagcagttga aatttaggac ttaagtgggc atctcgttta    11280

atttttaatg gatttctatg ttgttggtta tggtgaagag gtgaaaagaa taaatattct    11340

gtgcagaaaa attattcagt cttcatgtga aaacactttg tccatagcaa ttactttatg    11400

aaaaagatgt ggtattactt tctttgctct taactgagac ctttaattta aagaacctat    11460

actttacaag tttttatttt caatgcatga aaaatgtagc agctatttca caacctttac    11520

ttttaaaatc catttttctt tttaatctca aatagttttt tcttaaaacc ttttgacttt    11580

ttatctaaat tgtaatagcc agagcacctt cccacaacta gaatatctca tcctttttgt    11640

cttttctttt tcctctcaaa atgcctactg ggaacttaat ttggagtcag attcttcatg    11700

ataaatctgg acttaatcaa aattcctcat atggtatatt gtatatatca cagtactgga    11760

tagtcctctg attaaataga tatttgatag tactttaagg tctatacttt tggatgaact    11820

taactgcttt ctccatttgt agtctcttga aaatacagaa atttcagaaa taatttataa    11880

gaatatcaag gattcaaatc atatcagcac aaacacctaa atacttgttt gctttgttaa    11940

acacatatcc cattttctat cttgataaac attggtgtaa agtagttgaa tcattcagtg    12000

ggtataagca gcatattctc aatactatgt ttcattaata attaatagag atatatgaac    12060

acataaaaga ttcaattata atcaccttgt ggatctaaat ttcagttgac ttgtcatctt    12120

gatttctgga gaccacaagg taatgaaaaa taattacaag agtcttccat ctgttgcagt    12180

attaaaatgg cgagtaagac accctgaaag gaaatgttct attcatggta caatgcaatt    12240

acagctagca ccaaattcaa cactgtttaa ctttcaacat attattttga tttatcttga    12300

tccaacattc tcagggagga ggtgcattga agttattaga aaacactgac ttagatttag    12360

ggtatgtctt aaaagcttat ttgcgggaag tactctagcc ttattcaaca gatcactgag    12420

aagcctggaa aaacaaatcc cggaaactaa ttattatgtg ccagttatat aaacaagaag    12480

actttgttgg gtacaaacca gtgattcctt gcctttgaaa aatgtgtcag atatcatgca    12540

ttaccagcag ttcaatgata taaggaaacc agagtaatag ctaaaacctt taaagctaaa    12600

ccaaagattt acaaattgcc tcttcatcca gtctttccca acctaaaaac tgagttctct    12660

aaaaatttta gtattttttt ctgaagaaaa gggaacatgg acatttatct aatcctcatt    12720

agaaatctga ctaatgataa caaggattta gacctcaagc acttcttacc aaaattcttg    12780

atatgacctt atagcaaatt actttcacct gttgaacttt cctttctttt attcccctgt    12840

acctcacctg cactgggcat attcaagttg cttatacaac actttactat tgtgttagaa    12900

aaatcatgac acatgatgaa tgtgtttgtg caacatgagc tgattcataa atgaaaatgt    12960

gcattgaaat tccacaatat tttaaaatta ggagtttatc tagcaattga acaaaattga    13020

ttaaatccat tatttgttag atcagctaaa ttacataagt tcattcatct gctcataaat    13080

ccatccattc ttccatctgg ctatccctta gtcaattcaa ataaatattt atggggcact    13140

ttgggtaagc caggtgctaa gaattcaatg caaaacaaga tagactcccc tgtccttgtt    13200

gaacttatat ttttggtaca aacaaaagca ataatcaaga aaaaataaaa aaagtactga    13260

ttgtgattaa taatatgaag aaattcaaca gagtattgta cttaacattt gattgatctg    13320

attttctcag ttgtctgaga acaaacattt gtgaaaatct cattgtagag ttcttacgat    13380

ggataggggg tcaactgtgt cattattgct tatcagctta tcccaaagac ctagtttatt    13440

accagattgc aaatagtgtt caataaatta ttcttattaa gggttgttat gtactctaaa    13500

acatttattg tggtcccttc actggttctg gtttacaaac ttacttttct atgatgacat    13560

agtatagaaa ttgagagtga atatttagaa gttcattttt attatatatt tttgaagtat    13620

tgatatgtag tgaattagaa atttaaaaag aaaacaaaac tgtccttcac tacagattga    13680

aaagcattat actaaaagac catttgctca gttatagtat ataaaggcca aatgacttaa    13740

aaacaaatta tgtaaggaga aggaaacaac catttattca gtgccactaa ctgtcagcca    13800

gttttttcag tggtcagtta atgactgcag tagtgttcta ccttgctcaa agcaccctcc    13860

tcaagttctg gcatctaagc tgacatcaga acacagagtt ggggctctct gtgggtcacc    13920

tctagcactt gatctcctca tgcagtgcat ggtgctctca cgtctatgct atgttcttat    13980

ggtctttagg taacaagaat aattttcttt cttttcctta ctatacattt tgctttctga    14040

aattcccttc tcgccaatcc aggtgaatgt cagaatgtga tttgacaact gtccaaagta    14100

ctcattcact gaggagtggt aaggccttcg cccaacctgc cttctctggg aatatactgc    14160

tgcctgaaca tatcattgtt tattgccagg cttgaacttc accaaattaa tttattaggg    14220

tcaacatcta aatattagaa ctatttcaga ttaattttta agtcgtatcc actttgggta    14280

ctagatcaaa ttgcaggtct ctgcttctgg cttgagccta tgtttagaga tgatgtgcat    14340

gaagacactc tttgcttttc ctttatgcaa aatgggcatt ttcaatcttt ttgtcattag    14400

taaaggtcag tgataaagga agtctgcatc aggggtccaa ttccttatgg ccagtttctc    14460

tattctgttc caaggttgtt tgtctccata tatcaacatt ggtcaggatt gaaagtgtgc    14520

aacaaggttt gaatgaataa gtgaaaatct tccactggtg acaggataaa atattccaat    14580

ggtttttatt gaagtacaat actgaattat gtttatggca tggtacctat atgtcacaga    14640

agtgatccca tcacttttac cttatag                                        14667


<210>  71
<211>  14667
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(14667)
<223>  CFTR exon 19 containing 3849 + 10 kb C-to-T mutation

<220>
<221>  misc_feature
<222>  (12191)..(12191)
<223>  3849 + 10 kb C-to-T mutation

<400>  71
gtgagatttg aacactgctt gctttgttag actgtgttca gtaagtgaat cccagtagcc       60

tgaagcaatg tgttagcaga atctatttgt aacattatta ttgtacagta gaatcaatat      120

taaacacaca tgttttatta tatggagtca ttatttttaa tatgaaattt aatttgcaga      180

gtcctgaacc tatataatgg gtttatttta aatgtgattg tacttgcaga atatctaatt      240

aattgctagg ttaataacta aagaagccat taaataaatc aaaattgtaa catgttttag      300

atttcccatc ttgaaaatgt cttccaaaaa tatcttattg ctgactccat ctattgtctt      360

aaattttatc taagttccat tctgccaaac aagtgatact ttttttctag cttttttcag      420

tttgtttgtt ttgtttttct ttgaagtttt aattcagaca tagattattt tttcccagtt      480

atttactata tttattaagc atgagtaatt gacattattt tgaaatcctt cttatggatc      540

ccagcactgg gctgaacaca tagaaggaac ttaatatata ctgatttctg gaattgattc      600

ttggagacag ggatggtcat tatccatata cttcaggctc cataaacata tttcttaatt      660

gccttcaaat ccctattctg gactgctcta taaatctaga caagagtatt atatattttg      720

attgatattt tttagataaa ataaaaggga gctgaaaact gaattgcaaa ctgaatttta      780

aaactttatc tctctgtggt taattgcaaa cacagataca aaaatataga gagagataca      840

gttagtaaag atgttaggtc accgttacta acactgacat agaaacagtt ttgctcatga      900

gtttcagaat atatgagttt gattttgccc atggatttta gaatatttga taaacattta      960

atgcattgta caaattctgt gaaaacatat atataggatg tgcgaaaagt ccctgtgtat     1020

catgtgaaat ggcttaaaac agaacaccat aggtattcat atcagtgaat accataggta     1080

gctgaaagtg ttttttcctg gggtcgccaa gatgaatgcc aaaagtgata tcattattat     1140

aaacaatagc cagaataggt tggtataaac ctggtagaaa gccttgataa attgactttc     1200

tctcctcctg acatcctgcc acccctttgc tttgctgatg ctcatttgtc cactaaatta     1260

aactcaagca agccctagta aagtaataga atttgtggag tcctcattag tataggaagt     1320

ttccctgatg tgagattagt aattagagat gtagcaaaat gagaaagaag taatatgctt     1380

agatatttca ttttctctga acctgtatat acaaaatagg ccatgcgtgt tcagtaacta     1440

ttcactgcaa ggcactctct aggtactttg ggggaattgg aaattactca cataaggcta     1500

tggattgtgc catttgtcaa aagacaaaat gacaacaaat ttagtttaaa gacctcagtc     1560

agctttattt tctattctag atttggacag tccttcattt cacaaattgg agtaagtgtt     1620

ccaataagtt gagcaaagga gcttggcttt atagacccaa aaaaagggcc aaaggaagca     1680

gaaacaaaga acaataagag aattggtcat ttcaaagtta cttttcttga aaggtgggga     1740

caaggagaca gaataataga aaagtcactg attggttaac attggattaa gaattaaaac     1800

agaggaaact ttaagattga agtttgaaac tgacttgttt gggaaatcag gctgtcttct     1860

ttcttgattt cttagaaggc cggataacaa ctgagttttg ctttggtgaa catgggtgac     1920

tccattttta cttttagtct ggtctgttga ggcctcgtga gagagcttaa tctaaaacaa     1980

tgacttccta taatttttgt ttgacacatc caaagaggga ctctaatatt tattgagagc     2040

ttatcatatc ttaagtactg tttaaacact tttatttgct attacatttg atcttattat     2100

aactctaaag gcagaaatga ttgcttttat tttccacaat ggaggaaact gaggttcaat     2160

taagtgagta aggaagcagg gatcttaaac ccagatacca ttgctcctct ttaaaggtgg     2220

aagaacagaa aacatggggc aggggaagag agaaagtttc tgtcccagga catgataatc     2280

taaaagggaa aacgtaagat ccactgaaac ctgaggcaga tttattgtgg caataacaaa     2340

gcttaagttt cacagacctt catttgcctg agccaacttt gaaggccatg tatctaattt     2400

tgtttttata attctataat ctttattctt gaaaagagcc ctccctccaa atttacaagc     2460

tttgggcccc caaaatcctt gaaatgccct tgaataagag atatccaggt aaatgctatg     2520

ggaattcaga ggaggaagca gttagtatca gttggcggag agttaggcta ttaagagaag     2580

gttttatata ggaagtggca tttagaatga agctttgaga actgagctgt gtatttgaac     2640

aagtaaaggt ggtgttgcag aattttgctc cttagttcta ttaaaaaccc gggttcttgt     2700

cacatgatcc ggaaaattta ggcacacaga tacattgaag catgagtaga gcaggatttt     2760

attgggcaaa aaggaaaaaa agaaaactca gcaaatcgag atggagtctt gctcacagat     2820

tgaatcccag gccaccacaa aggaactgaa gagatcgggc ttctcccctg cataaggtgc     2880

aaattcccca tggctccacc cacttcccct tagtgtgcat gtggggctcc agtccacggt     2940

gggcatgccc agacaagcct tgggcaggtt ccctcatctg tgcaaaagca tctgatgtaa     3000

acacttgagg ggtggttcgg agattctctg ggaccctttt attttcttat ctgcctaggc     3060

atttggctgt ctcagtgggt gggaaagggt gctccaggca aagggcataa catgaggcaa     3120

agggcatgca cagaaaacag tgactggttc agtcaggttg ggggatgcca aaggaagtaa     3180

tgggagacaa gattggagca agatagataa gagattgtgg attttttttc ttttttatct     3240

atataaatac agagacaggg tctcactatg ttgcccaggc tggtctcaaa ctcctggcct     3300

caagtgatcc tcccacctca tcctcccaaa gtgctaggat tacaggcatg aggcactgtg     3360

cccaacctcc aattttggat tttgagagct aaagcaatat agtcgaaaac tcagataatc     3420

caggtagatt ttgctattag gtgctatttg gttcctggta cagagctaaa acccttggaa     3480

tttcctaagt gataagagct acaggagcat cttttgttat atgtttcccc ccctagttcc     3540

tgaaatagct ctagagaaat acaggtgaat aacatccttt gttattcata tcaagcccct     3600

atcaaccata ccccagtttc tatttatgaa gtggcttttg ggaagtccct aaagacagga     3660

gtggggaaag gctggttgtc agggggatgg gttgaaactt tcatcttccc cccttgacct     3720

ccagggaggg atgagtggct gaaaattgtg taaaatcaac aatggccagt gatttaatca     3780

accatgccta tgtaatgaag ccacccgata agccttaact ggaacttttt ggagagcctc     3840

caggctggtg aagacattga ggtgctcaga aggtggtatt ccagagagag cacagaatct     3900

ctgttcccct tcccacattc attttgctat gcatctctcc catctggctg ttcttgagag     3960

gtatccgttt ataataaact ggtaacctag taagtaaact gttaccctga gttctgtgag     4020

ccattctagc aaattatcaa acctaaagag ttcatggata cgtgcaattt acagatgcac     4080

agtcagaagc acagatgaca atctgggctt gccattggca tttgaagtgt gttgggaggc     4140

agtcttacag gaatgagccc ttatcctgtg gggtctatgc taataacaga cagttgtcag     4200

cattgcttgg tgtcgaaaac ccacattgtt ggtgtcagaa gtattgtcag taggataggg     4260

aaaacagttt gttttctttt tttagtggtc tttggtcatc tttaagagca gggcttctca     4320

aagtgtggtc cttgaaccag catcacctgt accacgtaag aacttatgag aaatgttcat     4380

tcttgggccc caacaaagaa ttaaaaattc tgagggtgtg aacggggtct gagtttcagc     4440

acaacttccc gaccatgctg atgcattctt gcccaagcat gaaagccctc ccttgtttaa     4500

gaaggccatt agggccgggt gtggtggctc atgcttgtaa tcgagcactt tgagaggaca     4560

tagtgggagg atcacttgag ccctggagtt ctagacaagc ctgggcaaca tggcaaaatg     4620

ctgtctccac aaaaatcaca aaaattaggt gggcgtgtgt tgtgtgccta taggcccagc     4680

tacttaggag actgaggcag gaggatcgct tgagcccagg agattaaggc tgcagcgagc     4740

tgtgatggca ccactacagc ctggatgaca gagtgagaca ctgtctcaaa aaaaaaaaag     4800

aaaaagaaaa agaaaaaaga aaggaaaatg aaaaagaacg ccattaggta taaaggagca     4860

atggtaaaag accagttgca aaaggttagg gaatgggtgg ttactgaaat aagaagctat     4920

gtagaacact agtgttggtg gcaggaagta gaaagcaaga gcactgctct gtgggggatg     4980

gtcatagcaa atgcaatatg gaggcatttg cctctgcact gaggagaaaa ctatcttttc     5040

caagatagga ggaaaggaga taagtggaat taaagagaac ctttgagcac agagttggga     5100

aactgaaggt atttgtgttg tgctccctca atcttttaat tcaactataa gctaaaccca     5160

tgaaacttga gtagtttcag ttatctgact tttttcttct cttttgatac agtgttggct     5220

attctgggtc ttttgcctct ctttatgtac ttaagaatca gtttgccaat gtatgcaaaa     5280

taactggctg ggattttgat tgtgattggc ttgaatctat agatggagtt gggaaggact     5340

gacatcttga caatgttgaa gcttcctatt catcattatg aaatatttct ccatttgttt     5400

gattctttga tttcttttat cagaatttag ttttcctcat atagtctttt aaaatatttt     5460

gttatatttt gttcaagtat tttgtttttg aggaatgcca atgtaaatgg tattgtgatt     5520

ttaatttcaa attccaattt ttcattgctg ttatatagga aaatgatttt ttttgcatgt     5580

tagccttata tctttcaact ttgctataat caattattga tagtttcaag gattttttgg     5640

tcaattattt tgaatcttct acatagatta tcatcatctg aacttagttt tatttcttcc     5700

ttcccaatct gtataccttt atctcctttt cttatttcat tagctaggac ttccagtatg     5760

atgttgaaag tagtggtgag aggggatatc ttggtcttgt tcttgatctt agtgggaaaa     5820

cttcaagttt cttatcatta agtatgattt tagctggagg gtttttgtag aagttttttt     5880

tttttaagtt gaagaagtct ccttctattt ttagtttgct gatttttaaa aagaatcagg     5940

aatgggtgtt aaattttgtg aaatgctttt ctgcaactat tgatttgagc actttatttt     6000

tcttctttgg cttgttgatg tgaagtacat taattgattt ttgaatgctg aatcaacctt     6060

ttgtacctga gattaatccc gtttggttgt ggtatataat tatttgtata catgttgagt     6120

tcgatttgct aatacttttt gagaattttt gcattggtgt tcatgaaaaa atattggtgt     6180

gtagtttttt gtgacatctt tatctgctta tggttttaag gtaatgctgg cctcatagca     6240

tgagttaggg agtatttcct ctacttttac atttgagaag agattgcaga gaattagtaa     6300

aattcctact ttaaatattt tgtggaattc accagtgaac ccatctggac ctggtgcttt     6360

ctgttttgga aggtcattaa ttattttaaa atagatatag gcctattcag attacctatt     6420

ttttctcatg cgagttttag cagattgtct ttcaaggaat tggtctattt catttaggtt     6480

atcaaatatg tcaacgtaga gttattcata gtattctttt attatccttt taatgtgcaa     6540

gggatctgta gtgatgtccc cttttttgtt ttattgatat tagcaatttg tgtcacatct     6600

tttattttgc tttgttagcc aggctagaga tatctctatt tttgatgttt ttgatgaacc     6660

aactttttgt tttattgatt ttctctgttg atttcgtgat ttcaatttca tgatttttaa     6720

attatgctta catttgattt aatttgatct tcttttgcta gttatccaag gtggaagctt     6780

atattgttaa gatccttttg cattcttatg cattcaatga tgtaaatttc cctctaagca     6840

ctgctttttc tgcatctcac aaatattcat gagttgtatt ttcatgttca tttagtttga     6900

aatattttta aatttctctt gatatttctc ttttgaccca tgtgttactt agaagtgtgt     6960

tgtttaatca ccatttttaa aaattttcta gctatctttc tgttattgat ttctagttta     7020

attccattgt ggtctgagag catatattgt ataattttaa tttttataaa atttgttaag     7080

gtgtgattta tggcccagaa tgtggtctat cttggtgaat gttccatgta agctttggaa     7140

gactgtgtat tctgctatat ttgaatgagg tagtctatag acatcaatta tgtccagttg     7200

attgatggtg ctgttgaatt caactatgtc cttactgatt ttccacctgc tagatctgtc     7260

cattctttgc agagggacac tgaagtctcc aactctagta gtgaatattc tatttcttgt     7320

tacagtttta tcaacttctg cttcatgtct tttgatgctt tgttgctaga aacatacaca     7380

tgaagaattg gtatgtcttt tggagcatga cccatttatc ctcatataat gcccctcatt     7440

atttcctcgc cctgatgtct gttctctctg aaagaaatat agcctctcca ggtctctttt     7500

ggttggtgtt aaaatgactt aactttcttt atccccctta cttttagttt atatgtggtt     7560

ttaaatttaa agtgggtttc ttgtagacag caaatagttc agagttgttt ttcgatccac     7620

tttgacaatc tttgtctttt aattggtata tttggactat tgatatttta agtgattatt     7680

gatatagtta gataaacatc tactatattt attactgttt tctgtctgtt acactacttg     7740

ttctttgttt atatttttat tgtctactct ttttctttcc attgtggttt taatcgagca     7800

ttttatatgt ttccattttc ttttcttagc atagtaattc ttctttaaaa aaacattttt     7860

tagtggttgc ccctagagtt tgcaatatac atttacaact aatctaagtc cattttcaaa     7920

taatactaaa taatttcatg tgtagtgcaa gtacctttta ataataaaac actcccagtt     7980

ccaccttcca gtctcttgta ttatagctat aatttagttc acttacatat atgggtatac     8040

ctaagtatat acattatcat atttatgatt gaatatattg atgaaattat tttgaaaaaa     8100

ctgttatcgt taaatcaatt aagagtaaga aaaatagttc taattttatt ataaaatgaa     8160

ataccttcat ttattcattc tctaatacac tttctttctt tatgtagatc caagtttctg     8220

acctgtataa ttttcctttt ctctcttcag cttctttgaa catttcttac cagccagacc     8280

tactgacaac aattttcccc aatttttgtt tgtctgatag agactttatt tcttcttgac     8340

ttttgaagaa taattccaca gggcacagaa ctctagattg gtgatttctt cccctcaaac     8400

ccttaaatat ttcattccac tgccttcttg cttgcattgt ttctgagaag ttagatataa     8460

ttcttatctt tgcctttcta taggtaagat gttttttcct ctggcttcta tcaagatttt     8520

ttctttatga acatgatatg cctttctttt tgaacatgat atgcctttct ttttgaacat     8580

gatatgcctt tgtgtcggat tttttttggc attattctgc ttggttttct ctgagtttct     8640

tggatatgtg gtatggtatc tgacactaat ttggaaaaat tctcagtcat tattgcttca     8700

aatatttctt ctgttctttt ttttccttta ttctccttct ggtattccca ttacatgtat     8760

gttacagttt ttgtagtcat cccgctgttt tggatattct gtttttttca gttttttttt     8820

ccttcgcatt tcagtgttgg aagtttctat tgacatattc tcaacctcag agattctttc     8880

ttcagctgtg ttcagtctac caatgagtcc atcaaaggca ttttacattt ttattacaga     8940

atttttgacc tatagaattt cttttgattc catctttgaa tctccatttc tcttctgctt     9000

ttcatctgtt cttgcatgtt gcctactttt tccatgaaaa cctttagctt tttttttttt     9060

tctttttgag gtggagtctc actgttgccc aggctggagt gcagtggtgt gatcttggct     9120

cactgcaacc tctgcctcct gggttcaagt gattctcctc ctcagcctcc caagtagctg     9180

ggattacagg tgcctgccac catgcctgag taatttttgt atttttagta gagatggggt     9240

tttatcatgt tggccaggcg ggtcttgaac tcctaacctc aagtgatctg cccaccttag     9300

cctcccaaat tgctgggatt ataggtgtga gccaccatgc cctgccttta gcatgttaat     9360

catagttgtt ttaaattcct gatctgttaa ttccaacatc cctgtcatat ctgactgtgg     9420

ttctgatgct tgctctgtgt tttcaaatgg tgtttttttt tttttgcctt ttagtaagcc     9480

ttgtaatttt ttattgaaag gtggacatga tgtgctgggt aaaaggaact gtagtaaata     9540

ggcctttagt aatgtactgg taggtgtagc agagggtgag ggaagtattc tgtagtccta     9600

tgattaggtt ttagtctttt agtgagcctg tgcgcctgca gcttggaagc acttgtgaag     9660

tgttttttca ccccttttgg tgggacatag tgactagtgt gagcgggagt tgagtatttc     9720

ccttccccta ggtcagttag gctctgaaaa aaccctgata ggttaggcat ggtaaaatag     9780

tctcttttga gggcaggcat tgttataaga atagaatgct ctggggccag gtgcggtggc     9840

tcacgcctgt aatccccgca ctttgggagg ctaaggcagg tggatcacct gaggtcagga     9900

gttcgagacc agcctggcca acatggtgaa accccgtctc tactaaaaat acaaaaatca     9960

gccaggtgtg gtggcacaca cctataatcc cagctactca ggaggctgag gcaggagaac    10020

tgcttgaacc cagtaagtgg aggttacagt gacccaagat tgtgccactg cagtctagtc    10080

tgggtgacag agcaagactc cgtctcaaaa aaaaaagaat gctctggcat atttgaaaat    10140

ggttactttt cccttttttt ctctgatctt cactgtgaga acctggtaag catcctatag    10200

gcaaaattca taaaagtata gaagtcggcc agtgacttgg acccacttgg aattttcttg    10260

ctctcacatc atgcacactg aatctccagc aatttttcac ttacagttta ggttttccta    10320

ccctactact ggttctctca gaggtttctg cttattggtt tctgttttgt aagttgtgat    10380

tctctgtacc taactgcctg tctcccattt tggggggcag tggtttgccc tgtgacctca    10440

cttctctgac agatctaaga aaagttgttt atttttcagt gtgctctgct ttttacttgt    10500

tacgatgaag ccaaccactt tcagaatttc tacaaaccag atcagaatct ggaagtcctg    10560

tttttttatt ttttttatcc ctttgtttag catgttacct atcttaacac attttaaata    10620

agtgaatgca tagcttatat ctacttctag gttatatgct tccttagaat aggaattgat    10680

tcttaaaatg tcgttctgct cacgcctgta attccagcac tttgggaggc caaggcaggc    10740

ggatcacttg gggtcaggag ttcaagacca gcctggtcaa catggtaaaa ccctgtgcct    10800

gcaaaaaata caaaaattag ctgggcatgg tggtggccat ctgtaatccc agctactagg    10860

gaagctaagg catgagaatc acttgaacct gggaggtgga ggttgcagtg agctgagatc    10920

gcgccactgc actccagcct gggtgacaag agcaaaactc catctcataa ataaataaat    10980

aaataaataa ataaataata aaaataaaaa aataaaataa aacaaaaatt ttattctgag    11040

cagtctctga agaatataaa ttctactgcc ttgcctttag aacttataac agcatctcgc    11100

aaactatcac aagatgctcc aaacatactt cttatgtgct gaattaagaa gtcaactcaa    11160

atttagtata ctagtaatat ttttggatat cccaaaacac tgccagctca gctttaggct    11220

gcccttcttg ggggggaaaa aagcagttga aatttaggac ttaagtgggc atctcgttta    11280

atttttaatg gatttctatg ttgttggtta tggtgaagag gtgaaaagaa taaatattct    11340

gtgcagaaaa attattcagt cttcatgtga aaacactttg tccatagcaa ttactttatg    11400

aaaaagatgt ggtattactt tctttgctct taactgagac ctttaattta aagaacctat    11460

actttacaag tttttatttt caatgcatga aaaatgtagc agctatttca caacctttac    11520

ttttaaaatc catttttctt tttaatctca aatagttttt tcttaaaacc ttttgacttt    11580

ttatctaaat tgtaatagcc agagcacctt cccacaacta gaatatctca tcctttttgt    11640

cttttctttt tcctctcaaa atgcctactg ggaacttaat ttggagtcag attcttcatg    11700

ataaatctgg acttaatcaa aattcctcat atggtatatt gtatatatca cagtactgga    11760

tagtcctctg attaaataga tatttgatag tactttaagg tctatacttt tggatgaact    11820

taactgcttt ctccatttgt agtctcttga aaatacagaa atttcagaaa taatttataa    11880

gaatatcaag gattcaaatc atatcagcac aaacacctaa atacttgttt gctttgttaa    11940

acacatatcc cattttctat cttgataaac attggtgtaa agtagttgaa tcattcagtg    12000

ggtataagca gcatattctc aatactatgt ttcattaata attaatagag atatatgaac    12060

acataaaaga ttcaattata atcaccttgt ggatctaaat ttcagttgac ttgtcatctt    12120

gatttctgga gaccacaagg taatgaaaaa taattacaag agtcttccat ctgttgcagt    12180

attaaaatgg tgagtaagac accctgaaag gaaatgttct attcatggta caatgcaatt    12240

acagctagca ccaaattcaa cactgtttaa ctttcaacat attattttga tttatcttga    12300

tccaacattc tcagggagga ggtgcattga agttattaga aaacactgac ttagatttag    12360

ggtatgtctt aaaagcttat ttgcgggaag tactctagcc ttattcaaca gatcactgag    12420

aagcctggaa aaacaaatcc cggaaactaa ttattatgtg ccagttatat aaacaagaag    12480

actttgttgg gtacaaacca gtgattcctt gcctttgaaa aatgtgtcag atatcatgca    12540

ttaccagcag ttcaatgata taaggaaacc agagtaatag ctaaaacctt taaagctaaa    12600

ccaaagattt acaaattgcc tcttcatcca gtctttccca acctaaaaac tgagttctct    12660

aaaaatttta gtattttttt ctgaagaaaa gggaacatgg acatttatct aatcctcatt    12720

agaaatctga ctaatgataa caaggattta gacctcaagc acttcttacc aaaattcttg    12780

atatgacctt atagcaaatt actttcacct gttgaacttt cctttctttt attcccctgt    12840

acctcacctg cactgggcat attcaagttg cttatacaac actttactat tgtgttagaa    12900

aaatcatgac acatgatgaa tgtgtttgtg caacatgagc tgattcataa atgaaaatgt    12960

gcattgaaat tccacaatat tttaaaatta ggagtttatc tagcaattga acaaaattga    13020

ttaaatccat tatttgttag atcagctaaa ttacataagt tcattcatct gctcataaat    13080

ccatccattc ttccatctgg ctatccctta gtcaattcaa ataaatattt atggggcact    13140

ttgggtaagc caggtgctaa gaattcaatg caaaacaaga tagactcccc tgtccttgtt    13200

gaacttatat ttttggtaca aacaaaagca ataatcaaga aaaaataaaa aaagtactga    13260

ttgtgattaa taatatgaag aaattcaaca gagtattgta cttaacattt gattgatctg    13320

attttctcag ttgtctgaga acaaacattt gtgaaaatct cattgtagag ttcttacgat    13380

ggataggggg tcaactgtgt cattattgct tatcagctta tcccaaagac ctagtttatt    13440

accagattgc aaatagtgtt caataaatta ttcttattaa gggttgttat gtactctaaa    13500

acatttattg tggtcccttc actggttctg gtttacaaac ttacttttct atgatgacat    13560

agtatagaaa ttgagagtga atatttagaa gttcattttt attatatatt tttgaagtat    13620

tgatatgtag tgaattagaa atttaaaaag aaaacaaaac tgtccttcac tacagattga    13680

aaagcattat actaaaagac catttgctca gttatagtat ataaaggcca aatgacttaa    13740

aaacaaatta tgtaaggaga aggaaacaac catttattca gtgccactaa ctgtcagcca    13800

gttttttcag tggtcagtta atgactgcag tagtgttcta ccttgctcaa agcaccctcc    13860

tcaagttctg gcatctaagc tgacatcaga acacagagtt ggggctctct gtgggtcacc    13920

tctagcactt gatctcctca tgcagtgcat ggtgctctca cgtctatgct atgttcttat    13980

ggtctttagg taacaagaat aattttcttt cttttcctta ctatacattt tgctttctga    14040

aattcccttc tcgccaatcc aggtgaatgt cagaatgtga tttgacaact gtccaaagta    14100

ctcattcact gaggagtggt aaggccttcg cccaacctgc cttctctggg aatatactgc    14160

tgcctgaaca tatcattgtt tattgccagg cttgaacttc accaaattaa tttattaggg    14220

tcaacatcta aatattagaa ctatttcaga ttaattttta agtcgtatcc actttgggta    14280

ctagatcaaa ttgcaggtct ctgcttctgg cttgagccta tgtttagaga tgatgtgcat    14340

gaagacactc tttgcttttc ctttatgcaa aatgggcatt ttcaatcttt ttgtcattag    14400

taaaggtcag tgataaagga agtctgcatc aggggtccaa ttccttatgg ccagtttctc    14460

tattctgttc caaggttgtt tgtctccata tatcaacatt ggtcaggatt gaaagtgtgc    14520

aacaaggttt gaatgaataa gtgaaaatct tccactggtg acaggataaa atattccaat    14580

ggtttttatt gaagtacaat actgaattat gtttatggca tggtacctat atgtcacaga    14640

agtgatccca tcacttttac cttatag                                        14667


<210>  72
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  CFTR exon 19 wild-type oligo

<400>  72
gtcttactcg ccatttta                                                     18


<210>  73
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  CFTR exon 19 3849 + 10 kb C-to-T mutation oligo


<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  3849 + 10 kb C-to-T mutation

<400>  73
gtcttactca ccatttta                                                     18


<210>  74
<211>  3733
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<222>  (1)..(3733)
<223>  wild-type Mus musculus dystrophin intron 22, exon 23 and intron 
       23 sequences

<220>
<221>  Intron
<222>  (1)..(913)
<223>  intron 22

<220>
<221>  exon
<222>  (914)..(1126)
<223>  exon 23

<220>
<221>  Intron
<222>  (1127)..(3733)
<223>  intron 23

<400>  74
gtctgtggac atttgaatat cataaataac aaagaacatg tcttatcagt caagagatca       60

tattgatata ttaaacttaa ggtaataatg aaaaagtaaa gataataatg aaaaatcata      120

gattatgagt tggaaaaata aacagaacaa tttgaccaaa aacatgactt tttcttattt      180

ttttctatat attattttat aaatatacag acataaatag atatatattt ttaaattaaa      240

agtactgtat taaaggaaag gtataatttc atttcatatt tagtgacata agatatgaag      300

tatgattatt aaaattaaat cacattattt tattataatt actttatttt taattcctaa      360

tttctttaag cttaggtaaa atcaatggat ttatataatt agttagaatt taaatattaa      420

caaactataa cactatgatt aaatgcttga tattgagtag ttattttaat agcctaagtc      480

tggaaattaa atactagtaa gagaaacttc tgtgatgtga ggacatataa agactaattt      540

ttttgttgat tctaaaaatc ccatgttgta tacttattct ttttaaatct gaaaatatat      600

taatcatata ttgcctaaat gtcttaataa tgtttcactg taggtaagtt aaaatgtatc      660

acatatataa taaacatagt tattaatgca tagatattca gtaaaattat gacttctaaa      720

tttctgtcta aatataatat gccctgtaat ataatagaaa ttattcataa gaatacatat      780

atattgcttt atcagatatt ctactttgtt tagatctcta aattacataa acttttattt      840

accttcttct tgatatgaat gaaactcatc aaatatgcgt gttagtgtaa atgaacttct      900

atttaatttt gag gct ctg caa agt tct ttg aaa gag caa caa aat ggc         949
               Ala Leu Gln Ser Ser Leu Lys Glu Gln Gln Asn Gly            
               1               5                   10                     

ttc aac tat ctg agt gac act gtg aag gag atg gcc aag aaa gca cct        997
Phe Asn Tyr Leu Ser Asp Thr Val Lys Glu Met Ala Lys Lys Ala Pro           
        15                  20                  25                        

tca gaa ata tgc cag aaa tat ctg tca gaa ttt gaa gag att gag ggg       1045
Ser Glu Ile Cys Gln Lys Tyr Leu Ser Glu Phe Glu Glu Ile Glu Gly           
    30                  35                  40                            

cac tgg aag aaa ctt tcc tcc cag ttg gtg gaa agc tgc caa aag cta       1093
His Trp Lys Lys Leu Ser Ser Gln Leu Val Glu Ser Cys Gln Lys Leu           
45                  50                  55                  60            

gaa gaa cat atg aat aaa ctt cga aaa ttt cag gtaagccgag gtttggcctt     1146
Glu Glu His Met Asn Lys Leu Arg Lys Phe Gln                               
                65                  70                                    

taaactatat tttttcacat agcaattaat tggaaaatgt gatgggaaac agatatttta     1206

cccagagtcc ttcaaagata ttgatgatat caaaagccaa atctatttca aaggattgca     1266

acttgcctat ttttcctatg aaaacagtaa tgtgtcatac cttcttggat tgtctgtata     1326

aatgaattga ttttttttca ccaactccaa gtatacttaa cattttaaca taataattta     1386

aaatatcctt attccattat gttcattttt taagttgtag atatgattta gctcacagca     1446

tacatatata cacatgtatt acatatgcat atattatata tatggcagac atatgttttc     1506

actaccatat ttcacttttg aattatgaat atatgtttaa tttctgccat atttccttcc     1566

ctacattgac ttctattaat ttagtatttc agtagttcta acacattaat aataacctag     1626

actcaataca gtaatctaac aattatattt gtgcctgtaa ttctaagtta gttaaattca     1686

taggttgtgt ttctcatagt tggccatttg tgaaatataa taatatccga aaagaaagtt     1746

caaaaatgtc atgacttcat atagagttat tgaaacagtg cccttacttt cattctggcc     1806

atgctagtga cttgatcatt cttgtatttt acagctaaaa cactaccaaa agtgtcaaat     1866

ccatgatcta catgtttgac tgaggctagc agcacttatt ccacccttat atgaagcctt     1926

taagagaaag tatatttgtt tgctattttt aacttcttga aggaacatac aatctttgtt     1986

tcaagagctc atcctctttc atgctagtaa attttggtgg cattgcatcc atgtctgact     2046

ctgaatctgt ttctgtctat cctgctccct aacactgtac catcttcctt tttgaaaaaa     2106

aaatattgaa ttattttatt tatttacttt ccaaagttgc tcctgcctgt tcctccttct     2166

ccaagttctt cagtcccccc tgctccccac cgatgagagg gaaaggtcct gaattcactg     2226

ggctccatgg gggtcctttt gcattttctt aaccttctta ataaaatagg ccttctagaa     2286

ttatatcata tacattgtga tatgacaaat gataaagtat attgttcaga gttttacctt     2346

gttcatattt gcaatgtccc cctgtcatgc tggatattct ttgattgggt atatttgcta     2406

acagattaag tatatttatc ttcgttaagc agtataactt attaagaaag aactctatta     2466

atatgagaaa taactaatga aacaccactc cacaggtgat ttcagccact ttatgaactg     2526

ctggaagcaa aaatgagatc tttgcaacat gaagcagttg ctcagttcat taaactgtgt     2586

tcaatatttc agccataaca tacattagag aatgatttat attgttcaaa catttggtgc     2646

tctatttttg catgacgtgg gattaaacac agcaccaaca atcaaacaat tgcaaagatg     2706

tattacaagt attttttctt tttaaaacag gaaagtatac ttatatttcc attgtccaaa     2766

ccatcatgaa agggatagag attactgaca caaatttaga gaaaggattt gagtggagta     2826

agaattaaat gaaccaaaga agaattaatg tattcatcaa gaagtcatgg aggtgaaatt     2886

ggccttgaat gataccacta aggagagaat gttgagatcc ttatatttag tcaattgttt     2946

ttaaatctgt agttattaac cacattttaa tcatattgaa agggaaattt tctgtgatgc     3006

atgtattttc aatataaatt ttagaaaaga agacaattat aacttgattt tgtgaattac     3066

atggaactaa agaaatgaca gatttacatt tgaaaattga ctgaactaaa gtacataaat     3126

aaaagtcata cagaaaaatg tgggaggtgc ttgtccattt ataaaggaca aaaatgccat     3186

ttgttgccta atcattattt cttattggtc agaccaataa gaaatcaaga gctttgactt     3246

taaaggtaag aaaatcttac cttaaaatcc ccaactgaag ggactgttta aactgtcaac     3306

tgcagaaaac aagttatgga agttcaggtt tagggaaact ataaacacac cataacattg     3366

agtttatgtg catagtttgt tttatgtaca gtgagagtaa attgttagta ttatcatgag     3426

ttgttttgaa acttcaaatt tctctagagg ggtatgattt aatgttctca agaggaacat     3486

aataaaacca tatctggtat tagtttttat ttttaacaat agcagacttc atacaccaat     3546

gttcacagtg tagaccataa aatgcagtct tagtaaaaat attattctct ataaagctac     3606

aatgagacct ccctcaaaca tacattgttt ttttttttct aacttatgtt tggatatatc     3666

atcatgatga actatgttaa aaacaatcag agcttagtaa tactttcata ttgctttttt     3726

attccag                                                               3733


<210>  75
<211>  3733
<212>  DNA
<213>  Mus musculus


<220>
<221>  misc_feature
<222>  (1)..(3733)
<223>  mdx Mus musculus dystrophin intron 22, exon 23 and intron 23 
       sequences

<220>
<221>  Intron
<222>  (1)..(913)
<223>  intron 22

<220>
<221>  exon
<222>  (914)..(1126)
<223>  exon 23

<220>
<221>  misc_feature
<222>  (941)..(941)
<223>  mdx C to T nonsense mutation

<220>
<221>  Intron
<222>  (1127)..(3733)
<223>  intron 23

<400>  75
gtctgtggac atttgaatat cataaataac aaagaacatg tcttatcagt caagagatca       60

tattgatata ttaaacttaa ggtaataatg aaaaagtaaa gataataatg aaaaatcata      120

gattatgagt tggaaaaata aacagaacaa tttgaccaaa aacatgactt tttcttattt      180

ttttctatat attattttat aaatatacag acataaatag atatatattt ttaaattaaa      240

agtactgtat taaaggaaag gtataatttc atttcatatt tagtgacata agatatgaag      300

tatgattatt aaaattaaat cacattattt tattataatt actttatttt taattcctaa      360

tttctttaag cttaggtaaa atcaatggat ttatataatt agttagaatt taaatattaa      420

caaactataa cactatgatt aaatgcttga tattgagtag ttattttaat agcctaagtc      480

tggaaattaa atactagtaa gagaaacttc tgtgatgtga ggacatataa agactaattt      540

ttttgttgat tctaaaaatc ccatgttgta tacttattct ttttaaatct gaaaatatat      600

taatcatata ttgcctaaat gtcttaataa tgtttcactg taggtaagtt aaaatgtatc      660

acatatataa taaacatagt tattaatgca tagatattca gtaaaattat gacttctaaa      720

tttctgtcta aatataatat gccctgtaat ataatagaaa ttattcataa gaatacatat      780

atattgcttt atcagatatt ctactttgtt tagatctcta aattacataa acttttattt      840

accttcttct tgatatgaat gaaactcatc aaatatgcgt gttagtgtaa atgaacttct      900

atttaatttt gag gct ctg caa agt tct ttg aaa gag caa taa aat ggc         949
               Ala Leu Gln Ser Ser Leu Lys Glu Gln     Asn Gly            
               1               5                       10                 

ttc aac tat ctg agt gac act gtg aag gag atg gcc aag aaa gca cct        997
Phe Asn Tyr Leu Ser Asp Thr Val Lys Glu Met Ala Lys Lys Ala Pro           
            15                  20                  25                    

tca gaa ata tgc cag aaa tat ctg tca gaa ttt gaa gag att gag ggg       1045
Ser Glu Ile Cys Gln Lys Tyr Leu Ser Glu Phe Glu Glu Ile Glu Gly           
        30                  35                  40                        

cac tgg aag aaa ctt tcc tcc cag ttg gtg gaa agc tgc caa aag cta       1093
His Trp Lys Lys Leu Ser Ser Gln Leu Val Glu Ser Cys Gln Lys Leu           
    45                  50                  55                            

gaa gaa cat atg aat aaa ctt cga aaa ttt cag gtaagccgag gtttggcctt     1146
Glu Glu His Met Asn Lys Leu Arg Lys Phe Gln                               
60                  65                  70                                

taaactatat tttttcacat agcaattaat tggaaaatgt gatgggaaac agatatttta     1206

cccagagtcc ttcaaagata ttgatgatat caaaagccaa atctatttca aaggattgca     1266

acttgcctat ttttcctatg aaaacagtaa tgtgtcatac cttcttggat tgtctgtata     1326

aatgaattga ttttttttca ccaactccaa gtatacttaa cattttaaca taataattta     1386

aaatatcctt attccattat gttcattttt taagttgtag atatgattta gctcacagca     1446

tacatatata cacatgtatt acatatgcat atattatata tatggcagac atatgttttc     1506

actaccatat ttcacttttg aattatgaat atatgtttaa tttctgccat atttccttcc     1566

ctacattgac ttctattaat ttagtatttc agtagttcta acacattaat aataacctag     1626

actcaataca gtaatctaac aattatattt gtgcctgtaa ttctaagtta gttaaattca     1686

taggttgtgt ttctcatagt tggccatttg tgaaatataa taatatccga aaagaaagtt     1746

caaaaatgtc atgacttcat atagagttat tgaaacagtg cccttacttt cattctggcc     1806

atgctagtga cttgatcatt cttgtatttt acagctaaaa cactaccaaa agtgtcaaat     1866

ccatgatcta catgtttgac tgaggctagc agcacttatt ccacccttat atgaagcctt     1926

taagagaaag tatatttgtt tgctattttt aacttcttga aggaacatac aatctttgtt     1986

tcaagagctc atcctctttc atgctagtaa attttggtgg cattgcatcc atgtctgact     2046

ctgaatctgt ttctgtctat cctgctccct aacactgtac catcttcctt tttgaaaaaa     2106

aaatattgaa ttattttatt tatttacttt ccaaagttgc tcctgcctgt tcctccttct     2166

ccaagttctt cagtcccccc tgctccccac cgatgagagg gaaaggtcct gaattcactg     2226

ggctccatgg gggtcctttt gcattttctt aaccttctta ataaaatagg ccttctagaa     2286

ttatatcata tacattgtga tatgacaaat gataaagtat attgttcaga gttttacctt     2346

gttcatattt gcaatgtccc cctgtcatgc tggatattct ttgattgggt atatttgcta     2406

acagattaag tatatttatc ttcgttaagc agtataactt attaagaaag aactctatta     2466

atatgagaaa taactaatga aacaccactc cacaggtgat ttcagccact ttatgaactg     2526

ctggaagcaa aaatgagatc tttgcaacat gaagcagttg ctcagttcat taaactgtgt     2586

tcaatatttc agccataaca tacattagag aatgatttat attgttcaaa catttggtgc     2646

tctatttttg catgacgtgg gattaaacac agcaccaaca atcaaacaat tgcaaagatg     2706

tattacaagt attttttctt tttaaaacag gaaagtatac ttatatttcc attgtccaaa     2766

ccatcatgaa agggatagag attactgaca caaatttaga gaaaggattt gagtggagta     2826

agaattaaat gaaccaaaga agaattaatg tattcatcaa gaagtcatgg aggtgaaatt     2886

ggccttgaat gataccacta aggagagaat gttgagatcc ttatatttag tcaattgttt     2946

ttaaatctgt agttattaac cacattttaa tcatattgaa agggaaattt tctgtgatgc     3006

atgtattttc aatataaatt ttagaaaaga agacaattat aacttgattt tgtgaattac     3066

atggaactaa agaaatgaca gatttacatt tgaaaattga ctgaactaaa gtacataaat     3126

aaaagtcata cagaaaaatg tgggaggtgc ttgtccattt ataaaggaca aaaatgccat     3186

ttgttgccta atcattattt cttattggtc agaccaataa gaaatcaaga gctttgactt     3246

taaaggtaag aaaatcttac cttaaaatcc ccaactgaag ggactgttta aactgtcaac     3306

tgcagaaaac aagttatgga agttcaggtt tagggaaact ataaacacac cataacattg     3366

agtttatgtg catagtttgt tttatgtaca gtgagagtaa attgttagta ttatcatgag     3426

ttgttttgaa acttcaaatt tctctagagg ggtatgattt aatgttctca agaggaacat     3486

aataaaacca tatctggtat tagtttttat ttttaacaat agcagacttc atacaccaat     3546

gttcacagtg tagaccataa aatgcagtct tagtaaaaat attattctct ataaagctac     3606

aatgagacct ccctcaaaca tacattgttt ttttttttct aacttatgtt tggatatatc     3666

atcatgatga actatgttaa aaacaatcag agcttagtaa tactttcata ttgctttttt     3726

attccag                                                               3733


<210>  76
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Antisense exon 23 skipping inducing oligo


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  exon 23 skipping inducing oligonucleotide

<400>  76
aacctcggct tacctgaaat tttcg                                             25


<210>  77
<211>  1653
<212>  DNA
<213>  Hotaria parvula

<400>  77
atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga       60

accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt      120

gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc      180

gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta      240

tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt      300

gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt      360

tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa      420

aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga      480

tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat      540

tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga      600

tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg      660

catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt      720

gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt      780

cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac      840

aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg      900

attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct      960

aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat     1020

gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc     1080

gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa     1140

acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt     1200

tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct     1260

ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct     1320

ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa     1380

caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt     1440

cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat     1500

tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac     1560

gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata     1620

aaggccaaga agggcggaaa gatcgccgtg taa                                  1653


<210>  78
<211>  17578
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Intron
<222>  (1)..(13645)
<223>  intron 9

<220>
<221>  exon
<222>  (13646)..(13738)
<223>  intron 9

<220>
<221>  Intron
<222>  (13739)..(17578)
<223>  intron 10

<400>  78
gtgagagtgg ctggctgcgc gtggaggtgt ggggggctgc gcctggaggg gtagggctgt       60

gcctggaagg gtagggctgc gcctggaggt gcgcggttga gcgtggagtc gtgggactgt      120

gcatggaggt gtggggctcc ccgcacctga gcacccccgc ataacacccc agtcccctct      180

ggaccctctt caaggaagtt cagttcttta ttgggctctc cactacactg tgagtgccct      240

cctcaggcga gagaacgttc tggctcttct cttgcccctt cagcccctgt taatcggaca      300

gagatggcag ggctgtgtct ccacggccgg aggctctcat agtcagggca cccacagcgg      360

ttccccacct gccttctggg cagaatacac tgccacccat aggtcagcat ctccactcgt      420

gggccatctg cttaggttgg gttcctctgg attctgggga gattgggggt tctgttttga      480

tcagctgatt cttctgggag caagtgggtg ctcgcgagct ctccagcttc ctaaaggtgg      540

agaagcacag acttcggggg cctggcctgg atccctttcc ccattcctgt ccctgtgccc      600

ctcgtctggg tgcgttaggg ctgacataca aagcaccaca gtgaaagaac agcagtatgc      660

ctcctcacta gccaggtgtg ggcgggtggg tttcttccaa ggcctctctg tggccgtggg      720

tagccacctc tgtcctgcac cgctgcagtc ttccctctgt gtgtgctcct ggtagctctg      780

cgcatgctca tcttcttata agaacaccat ggcagctggg cgtagtggct cacgcctata      840

atcccagcac tttgggaggc tgaggcaggc agatcacgag gtcaggagtt cgagaccaac      900

ctgaccaaca gggtgaaacc tcgtctctac taaaaataca aaaatacctg ggcgtggtgg      960

tggtgcgcgc ctataatccc agctactcag gaggctgagg caggagaatc gcttgaaccc     1020

aggaggcaga ggttgcagtg agccgagata gtgccactgc actccagttt gagcaacaga     1080

gcgagactct gtctcaaaac aaaataaaac aaaccaaaaa aacccaccat ggcttagggc     1140

ccagcctgat gacctcattt ttcacttagt cacctctcta aaggccctgt ctccaaatag     1200

agtcacattc taaggtacgg gggtgttggg gaggggggtt agggcttcaa catgtgaatt     1260

tgcggggacc acaattcagc ccaggacccc gctcccgcca cccagcactg gggagctggg     1320

gaagggtgaa gaggaggctg ggggtgagaa ggaccacagc tcactctgag gctgcagatg     1380

tgctgggcct tctgggcact gggcctcggg gagctagggg gctttctgga accctgggcc     1440

tgcgtgtcag cttgcctccc ccacgcaggc gctctccaca ccattgaagt tcttatcact     1500

tgggtctgag cctggggcat ttggacggag ggtggccacc agtgcacatg ggcaccttgc     1560

ctcaaaccct gccacctccc cccacccagg atcccccctg cccccgaaca agcttgtgag     1620

tgcagtgtca catcccatcg ggatggaaat ggacggtcgg gttaaaaggg acgcatgtgt     1680

agaccctgcc tctgtgcatc aggcctcttt tgagagtccc tgcgtgccag gcggtgcaca     1740

gaggtggaga agactcggct gtgccccaga gcacctcctc tcatcgagga aaggacagac     1800

agtggctccc ctgtggctgt ggggacaagg gcagagctcc ctggaacaca ggagggaggg     1860

aaggaagaga acatctcaga atctccctcc tgatggcaaa cgatccgggt taaattaagg     1920

tccggccttt tcctgctcag gcatgtggag cttgtagtgg aagaggctct ctggaccctc     1980

atccaccaca gtggcctggt tagagacctt ggggaaataa ctcacaggtg acccagggcc     2040

tctgtcctgt accgcagctg agggaaactg tcctgcgctt ccactgggga caatgcgctc     2100

cctcgtctcc agactttcca gtcctcattc ggttctcgaa agtcgcctcc agaagcccca     2160

tcttgggacc accgtgactt tcattctcca gggtgcctgg ccttggtgct gcccaagacc     2220

ccagaggggc cctcactggc ctttcctgcc ttttctccca ttgcccaccc atgcaccccc     2280

atcctgctcc agcacccaga ctgccatcca ggatctcctc aagtcacata acaagcagca     2340

cccacaaggt gctcccttcc ccctagcctg aatctgctgc tccccgtctg gggttccccg     2400

cccatgcacc tctgggggcc cctgggttct gccataccct gccctgtgtc ccatggtggg     2460

gaatgtcctt ctctccttat ctcttccctt cccttaaatc caagttcagt tgccatctcc     2520

tccaggaagt cttcctggat tcccctctct cttcttaaag cccctgtaaa ctctgaccac     2580

actgagcatg tgtctgctgc tccctagtct gggccatgag tgagggtgga ggccaagtct     2640

catgcatttt tgcagccccc acaagactgt gcaggtggcc ggccctcatt gaatgcgggg     2700

ttaatttaac tcagcctctg tgtgagtgga tgattcaggt tgccagagac agaaccctca     2760

gcttagcatg ggaagtagct tccctgttga ccctgagttc atctgaggtt ggcttggaag     2820

gtgtgggcac catttggccc agttcttaca gctctgaaga gagcagcagg aatggggctg     2880

agcagggaag acaactttcc attgaaggcc cctttcaggg ccagaactgt ccctcccacc     2940

ctgcagctgc cctgcctctg cccatgaggg gtgagagtca ggcgacctca tgccaagtgt     3000

agaaaggggc agacgggagc cccaggttat gacgtcacca tgctgggtgg aggcagcacg     3060

tccaaatcta ctaaagggtt aaaggagaaa gggtgacttg acttttcttg agatattttg     3120

ggggacgaag tgtggaaaag tggcagagga cacagtcaca gcctccctta aatgccagga     3180

aagcctagaa aaattgtctg aaactaaacc tcagccataa caaagaccaa cacatgaatc     3240

tccaggaaaa aagaaaaaga aaaatgtcat acagggtcca tgcacaagag cctttaaaat     3300

gacccgctga agggtgtcag gcctcctcct cctggactgg cctgaaggct ccacgagctt     3360

ttgctgagac ctttgggtcc ctgtggcctc atgtagtacc cagtatgcag taagtgctca     3420

ataaatgttt ggctacaaaa gaggcaaagc tggcggagtc tgaagaatcc ctcaaccgtg     3480

ccggaacaga tgctaacacc aaagggaaaa gagcaggagc caagtcacgt ttgggaacct     3540

gcagaggctg aaaactgccg cagattgctg caaatcattg ggggaaaaac ggaaaacgtc     3600

tgttttcccc tttgtgcttt tctctgtttt cttctttgtg cttttctctg ttttcaggat     3660

ttgctacagt gaacatagat tgctttgggg ccccaaatgg aattattttg aaaggaaaat     3720

gcagataatc aggtggccgc actggagcac cagctgggta ggggtagaga ttgcaggcaa     3780

ggaggaggag ctgggtgggg tgccaggcag gaagagcccg taggccccgc cgatcttgtg     3840

ggagtcgtgg gtggcagtgt tccctccaga ctgtaaaagg gagcacctgg cgggaagagg     3900

gaattctttt aaacatcatt ccagtgcccg agcctcctgg acctgttgtc atcttgaggt     3960

gggcctcccc tgggtgactc tagtgtgcag cctggctgag actcagtggc cctgggttct     4020

tactgctgac acctaccctc aacctcaacc actgcggcct cctgtgcacc ctgatccagt     4080

ggctcatttt ccactttcag tcccagctct atccctattt gcagtttcca agtgcctggt     4140

cctcagtcag ctcagaccca gccaggccag cccctggttc ccacatcccc tttgccaagc     4200

tcatccccgc cctgtttggc ctgcgggagt gggagtgtgt ccagacacag agacaaagga     4260

ccagctttta aaacattttg ttggggccag gtgtggtggc tcacacctaa tcccaacacc     4320

tggggaggcc aaggcagaag gatcacttga gtccaggagt tcaagaccag cctgggcaac     4380

atagggagac cctgtctcta caattttttt tttaattagc tgggcctgtt ggcactctcc     4440

tgtagttcca gctactctag aggctgaggt gggaggactg cttgagcctg ggaggtcagg     4500

gctgcaatga gccatgttca caccactgaa cgccagcctg ggcgagaccc tgtatcaaaa     4560

aagtaaagta aaatgaatcc tgtacgttat attaaggtgc cccaaattgt acttagaagg     4620

atttcatagt tttaaatact tttgttattt aaaaaattaa atgactgcag catataaatt     4680

aggttcttaa tggaggggaa aaagagtaca agaaaagaaa taagaatcta gaaacaaaga     4740

taagagcaga aataaaccag aaaacacaac cttgcactcc taacttaaaa aaaaaaatga     4800

agaaaacaca accagtaaaa caacatataa cagcattaag agctggctcc tggctgggcg     4860

cggtggcgca tgcctgtaat cccaacactt tgggaggccg atgctggagg atcacttgag     4920

accaggagtt caaggttgca gtgagctatg atcataccac tacaccctag cctgggcaac     4980

acagtgagac tgagactcta ttaaaaaaaa aatgctggtt ccttccttat ttcattcctt     5040

tattcattca ttcagacaac atttatgggg cacttctgag caccaggctc tgtgctaaga     5100

gcttttgccc ccagggtcca ggccagggga caggggcagg tgagcagaga aacagggcca     5160

gtcacagcag caggaggaat gtaggatgga gagcttggcc aggcaaggac atgcaggggg     5220

agcagcctgc acaagtcagc aagccagaga agacaggcag acccttgttt gggacctgtt     5280

cagtggcctt tgaaaggaca gcccccaccc ggagtgctgg gtgcaggagc tgaaggagga     5340

tagtggaaca ctgcaacgtg gagctcttca gagcaaaagc aaaataaaca actggaggca     5400

gctggggcag cagagggtgt gtgttcagca ctaaggggtg tgaagcttga gcgctaggag     5460

agttcacact ggcagaagag aggttggggc agctgcaagc ctctggacat cgcccgacag     5520

gacagagggt ggtggacggt ggccctgaag agaggctcag ttcagctggc agtggccgtg     5580

ggagtgctga agcaggcagg ctgtcggcat ctgctgggga cggttaagca ggggtgaggg     5640

cccagcctca gcagcccttc ttggggggtc gctgggaaac atagaggaga actgaagaag     5700

cagggagtcc cagggtccat gcagggcgag agagaagttg ctcatgtggg gcccaggctg     5760

caggatcagg agaactgggg accctgtgac tgccagcggg gagaaggggg tgtgcaggat     5820

catgcccagg gaagggccca ggggcccaag catggggggg cctggttggc tctgagaaga     5880

tggagctaaa gtcactttct cggaggatgt ccaggccaat agttgggatg tgaagacgtg     5940

aagcagcaca gagcctggaa gcccaggatg gacagaaacc tacctgagca gtggggcttt     6000

gaaagccttg gggcgggggg tgcaatattc aagatggcca caagatggca atagaatgct     6060

gtaactttct tggttctggg ccgcagcctg ggtggctgct tccttccctg tgtgtattga     6120

tttgtttctc ttttttgaga cagagtcttg ctgggttgcc caggctggag tgcagtggtg     6180

cgatcatagc tcactgcagc cttgaagtcc tgagctcaag agatccttcc acctcagcct     6240

cctgagtagt tgggaccaca ggcttgcacc acagtgccca actaatttct tatatttttt     6300

gtagagatgg ggtttcactg tgtcgcccag gatggtcttg aactcctggg ctcaagtgat     6360

cctcctgcct cagcctcgca aattgctggg attacaggtg tgagccacca tgcccgacct     6420

tctcttttta agggcgtgtg tgtgtgtgtg tgtgtgtggg cgcactctcg tcttcacctt     6480

cccccagcct tgctctgtct ctacccagtc acctctgccc atctctccga tctgtttctc     6540

tctcctttta cccctctttc ctccctcctc atacaccact gaccattata gagaactgag     6600

tattctaaaa atacatttta tttatttatt ttgagacaga gtctcactct gtcacccagg     6660

ctggagtgca gtggtgcaat ctcggctcac tgcaacctcc gcctcccagg ttgaagcaac     6720

tctcctgcct cagcctccct agtagctggg attacaagca cacaccacca tgcctagcaa     6780

atttttatat ttttagtaga ggaggagtgt caccatgttt gccaagctgg tctcaaactc     6840

ctggcctcag gtgatctgcc taccttggtc tcccaaagtg ctgggattac aggtgtgagc     6900

caccacgcct gcccttaaaa atacattata tttaatagca aagccccagt tgtcacttta     6960

aaaagcatct atgtagaaca tttatgtgga ataaatacag tgaatttgta cgtggaatcg     7020

tttgcctctc ctcaatcagg gccagggatg caggtgagct tgggctgaga tgtcagaccc     7080

cacagtaagt ggggggcaga gccaggctgg gaccctcctc taggacagct ctgtaactct     7140

gagaccctcc aggcatcttt tcctgtacct cagtgcttct gaaaaatctg tgtgaatcaa     7200

atcattttaa aggagcttgg gttcatcact gtttaaagga cagtgtaaat aattctgaag     7260

gtgactctac cctgttattt gatctcttct ttggccagct gacttaacag gacatagaca     7320

ggttttcctg tgtcagttcc taagctgatc accttggact tgaagaggag gcttgtgtgg     7380

gcatccagtg cccaccccgg gttaaactcc cagcagagta ttgcactggg cttgctgagc     7440

ctggtgaggc aaagcacagc acagcgagca ccaggcagtg ctggagacag gccaagtctg     7500

ggccagcctg ggagccaact gtgaggcacg gacggggctg tggggctgtg gggctgcagg     7560

cttggggcca gggagggagg gctgggctct ttggaacagc cttgagagaa ctgaacccaa     7620

acaaaaccag atcaaggtct agtgagagct tagggctgct ttgggtgctc caggaaattg     7680

attaaaccaa gtggacacac acccccagcc ccacctcacc acagcctctc cttcagggtc     7740

aaactctgac cacagacatt tctcccctga ctaggagttc cctggatcaa aattgggagc     7800

ttgcaacaca tcgttctctc ccttgatggt ttttgtcagt gtctatccag agctgaagtg     7860

taatatatat gttactgtag ctgagaaatt aaatttcagg attctgattt cataatgaca     7920

accattcctc ttttctctcc cttctgtaaa tctaagattc tataaacggt gttgacttaa     7980

tgtgacaatt ggcagtagtt caggtctgct ttgtaaatac ccttgtgtct attgtaaaat     8040

ctcacaaagg cttgttgcct tttttgtggg gttagaacaa gaaaaagcca catggaaaaa     8100

aaatttcttt tttgtttttt tgtttgcttg tttttttgag acagagtttc actctgtcgc     8160

ccaggctgga gtgcagtggt gcgatctccg cccactgcaa gctccacctc ccgggttcat     8220

gctattctcc tgtctcagcc tcccaagtag ctgggactgc aggtgcccgc caccacacct     8280

ggctaatttt tttgtatttt tagtagagac ggggtttcac cgtgttagcc aggatggtct     8340

caatctcctg acctcgtcat ctgcctgcct cggcctccca aagtgctgag attacaggcg     8400

tgagccaccg tgcccggcca gaaaaaaaca tttctaagta tgtggcagat actgaattat     8460

tgcttaatgt cctttgattc atttgtttaa tttctttaat ggattagtac agaaaacaaa     8520

gttctcttcc ttgaaaaact ggtaagtttt ctttgtcaga taaggagagt taaataaccc     8580

atgacatttc cctttttgcc tcggcttcca ggaagctcaa agttaaatgt aatgatcact     8640

cttgtaatta tcagtgttga tgcccttccc ttcttctaat gttactcttt acattttcct     8700

gctttattat tgtgtgtgtt ttctaattct aagctgttcc cactcctttc tgaaagcagg     8760

caaatcttct aagccttatc cactgaaaag ttatgaataa aaaatgatcg tcaagcctac     8820

aggtgctgag gctactccag aggctgaggc cagaggacca cttgagccca ggaatttgag     8880

acctgggctg ggcagcatag caagactcta tctccattaa aactattttt ttttatttaa     8940

aaaataatcc gcaaagaagg agtttatgtg ggattcctta aaatcggagg gtggcatgaa     9000

ttgattcaaa gacttgtgca gagggcgaca gtgactcctt gagaagcagt gtgagaaagc     9060

ctgtcccacc tccttccgca gctccagcct gggctgaggc actgtcacag tgtctccttg     9120

ctggcaggag agaatttcaa cattcaccaa aaagtagtat tgtttttatt aggtttatga     9180

ggctgtagcc ttgaggacag cccaggacaa ctttgttgtc acatagatag cctgtggcta     9240

caaactctga gatctagatt cttctgcggc tgcttctgac ctgagaaagt tgcggaacct     9300

cagcgagcct cacatggcct ccttgtcctt aacgtgggga cggtgggcaa gaaaggtgat     9360

gtggcactag agatttatcc atctctaaag gaggagtgga ttgtacattg aaacaccaga     9420

gaaggaatta caaaggaaga atttgagtat ctaaaaatgt aggtcaggcg ctcctgtgtt     9480

gattgcaggg ctattcacaa tagccaagat ttggaagcaa cccaagtgtc catcaacaga     9540

caaatggata aagaaaatgt ggtgcatata cacaatggaa tactattcag ccatgaaaaa     9600

gaatgagaat ctgtcatttg aaacaacatg gatggaactg gaggacatta tgttaagtga     9660

aataagccag acagaaggac agacttcaca tgttctcaca catttgtggg agctaaaaat     9720

taaactcatg gagatagaga gtagaaggat ggttaccaga ggctgaggag ggtggagggg     9780

agcagggaga aagtagggat ggttaatggg tacaaaaacg tagttagcat gcatagatct     9840

agtattggat agcacagcag ggtgacgaca gccaacagta atttatagta catttaaaaa     9900

caactaaaag agtgtaactg gactggctaa catggtgaaa ccccgtctct actaaaaata     9960

caaaaattag ctgggcacgg tggctcacgc ctgtaatccc agcactttgg gaggccgagg    10020

cgggccgatc acgaggtcag gagatcgaga ccatcctagc taacatggtg aaaccccgtc    10080

tctactacaa atacaaaaaa aagaaaaaat tagccgggca tggtggtggg cgcctgtagt    10140

cccagctact cgggaggctg aggcaggaga atggcgtgaa cccgggaggc ggagcttgca    10200

gtgagccgag atcgcgccac tgcactccag cctgggcgac aaggcaagat tctatctcaa    10260

aaaaataaaa ataaaataaa ataaaataat aaaataaaat aaaataaaat aaaataaaat    10320

aaataaaata aaatgtataa ttggaatgtt tataacacaa gaaatgataa atgcttgagg    10380

tgatagatac cccattcacc gtgatgtgat tattgcacaa tgtatgtctg tatctaaata    10440

tctcatgtac cccacaagta tatacaccta ctatgtaccc atataaattt aaaattaaaa    10500

aattataaaa caaaaataaa taagtaaatt aaaatgtagg ctggacaccg tggttcacgc    10560

ctgtaatccc agtgctttgt gaggctgagg tgagagaatc acttgagccc aggagtttga    10620

gaccggcctg ggtgacatag cgagacccca tcatcacaaa gaatttttaa aaattagctg    10680

ggcgtggtag cacataccgg tagttccagc tacttgggag accgaggcag gaggattgct    10740

tgagcccagg agtttaaggc tgcagtgagc tacgatggcg ccactgcatt ccagcctggg    10800

tgacagagtg agagcttgtc tctattttaa aaataataaa aagaataaat aaaaataaat    10860

taaaatgtaa atatgtgcat gttagaaaaa atacacccat cagcaaaaag ggggtaaagg    10920

agcgatttca gtcataattg gagagatgca gaataagcca gcaatgcagt ttcttttatt    10980

ttggtcaaaa aaaataagca aaacaatgtt gtaaacaccc agtgctggca gcaatgtggt    11040

gaggctggct ctctcaccag ggctcacagg gaaaactcat gcaacccttt tagaaagcca    11100

tgtggagagt tgtaccgaga ggttttagaa tatttataac tttgacccag aaattctatt    11160

ctaggactct gtgttatgaa aataacccat catatggaaa aagctccttt cagaaagagg    11220

ttcatgggag gctgtttgta tttttttttt ctttgcatca aatccagctc ctgcaggact    11280

gtttgtatta ttgaagtaca aagtggaatc aatacaaatg ttggatagca ggggaacaat    11340

attcacaaaa tggaatggga catagtatta aacatagtgc ttctgatgac cgtagaccat    11400

agacaatgct taggatatga tatcacttct tttgttgttt tttgtatttt gagacgaagt    11460

ctcattctgt cacccaggct ggagttcagt ggcgccatct cagctcactg caacctccat    11520

ctcccgggtt caagctattc tccttcctca acctcccgag tagctgggtt gcgcaccacc    11580

atgcctggct aacttttgta tttttagtac agacggggtt tcaccacgtt ggccaggctg    11640

ctcttgaact cctgacgtca ggtgatccac cagccttgac ctcccaaagt gctaggatta    11700

caggagccac tgtacccagc ctaggatatg atatcacttc ttagagcaag atacaaaatt    11760

gcatgtgcac aataattcta ccaagtatag gtatacaggg gtagttatat ataaatgaga    11820

cttcaaggaa atacaacaaa atgcaatcgt gattgtgtta gggtggtaag aaaacggttt    11880

ttgctttgat gagctctgtt ttttaaaatc gttatatttt ctaataaaaa tacatagtct    11940

tttgaaggaa cataaaagat tatgaagaaa tgagttagat attgattcct attgaagatt    12000

cagacaagta aaattaaggg gaaaaaaaac gggatgaacc agaagtcagg ctggagttcc    12060

aaccccagat ccgacagccc aggctgatgg ggcctccagg gcagtggttt ccacccagca    12120

ttctcaaaag agccactgag gtctcagtgc cattttcaag atttcggaag cggcctgggc    12180

acggctggtc cttcactggg atcaccactt ggcaattatt tacacctgag acgaatgaaa    12240

accagagtgc tgagattaca ggcatggtgg cttacgcttg taatcggctt tgggaagccg    12300

aggtgggctg attgcttgag cccaggagtt tcaaactatc ctggacaaca tagcatgacc    12360

tcgtctctac aaaaaataca aaaaatttgc caggtgtggt ggcatgtgcc tgtggtccca    12420

gctacttggg aggctgaagt aggagaatcc cctgagccct gggaagtcga ggctgcactg    12480

agccgtgatg gtgtcactgc actccagcct gggtgacaaa gtgagaccct atctcacaaa    12540

gaaaaaaaac aaaacaaaaa acccaaagca cactgtttcc actgtttcca gagttcctga    12600

gaggaaaggt caccgggtga ggaagacgtt ctcactgatc tggcagagaa aatgtccagt    12660

ttttccaact ccctaaacca tggttttcta tttcatagtt cttaggcaaa ttggtaaaaa    12720

tcatttctca tcaaaacgct gatattttca cacctccctg gtgtctgcag aaagaacctt    12780

ccagaaatgc agtcgtggga gacccatcca ggccacccct gcttatggaa gagctgagaa    12840

aaagccccac gggagcattt gctcagcttc cgttacgcac ctagtggcat tgtgggtggg    12900

agagggctgg tgggtggatg gaaggagaag gcacagcccc cccttgcagg gacagagccc    12960

tcgtacagaa gggacacccc acatttgtct tccccacaaa gcggcctgtg tcctgcctac    13020

ggggtcaggg cttctcaaac ctggctgtgt gtcagaatca ccaggggaac ttttcaaaac    13080

tagagagact gaagccagac tcctagattc taattctagg tcagggctag gggctgagat    13140

tgtaaaaatc cacaggtgat tctgatgccc ggcaggcttg agaacagccg cagggagttc    13200

tctgggaatg tgccggtggg tctagccagg tgtgagtgga gatgccgggg aacttcctat    13260

tactcactcg tcagtgtggc cgaacacatt tttcacttga cctcaggctg gtgaacgctc    13320

ccctctgggg ttcaggcctc acgatgccat ccttttgtga agtgaggacc tgcaatccca    13380

gcttcgtaaa gcccgctgga aatcactcac acttctggga tgccttcaga gcagccctct    13440

atcccttcag ctcccctggg atgtgactcg acctcccgtc actccccaga ctgcctctgc    13500

caagtccgaa agtggaggca tccttgcgag caagtaggcg ggtccagggt ggcgcatgtc    13560

actcatcgaa agtggaggcg tccttgcgag caagcaggcg ggtccagggt ggcgtgtcac    13620

tcatcctttt ttctggctac caaag gtg cag ata att aat aag aag ctg gat      13672
                            Val Gln Ile Ile Asn Lys Lys Leu Asp           
                            1               5                             

ctt agc aac gtc cag tcc aag tgt ggc tca aag gat aat atc aaa cac      13720
Leu Ser Asn Val Gln Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His           
10                  15                  20                  25            

gtc ccg gga ggc ggc agt gtgagtacct tcacacgtcc catgcgccgt             13768
Val Pro Gly Gly Gly Ser                                                   
                30                                                        

gctgtggctt gaattattag gaagtggtgt gagtgcgtac acttgcgaga cactgcatag    13828

aataaatcct tcttgggctc tcaggatctg gctgcgacct ctgggtgaat gtagcccggc    13888

tccccacatt cccccacacg gtccactgtt cccagaagcc ccttcctcat attctaggag    13948

ggggtgtccc agcatttctg ggtcccccag cctgcgcagg ctgtgtggac agaatagggc    14008

agatgacgga ccctctctcc ggaccctgcc tgggaagctg agaataccca tcaaagtctc    14068

cttccactca tgcccagccc tgtccccagg agccccatag cccattggaa gttgggctga    14128

aggtggtggc acctgagact gggctgccgc ctcctccccc gacacctggg caggttgacg    14188

ttgagtggct ccactgtgga caggtgaccc gtttgttctg atgagcggac accaaggtct    14248

tactgtcctg ctcagctgct gctcctacac gttcaaggca ggagccgatt cctaagcctc    14308

cagcttatgc ttagcctgcg ccaccctctg gcagagactc cagatgcaaa gagccaaacc    14368

aaagtgcgac aggtccctct gcccagcgtt gaggtgtggc agagaaatgc tgcttttggc    14428

ccttttagat ttggctgcct cttgccagga gtggtggctc gtgcctgtaa ttccagcact    14488

ttgggagact aaggcgggag gttcgcttga gcccaggagt tcaagaccag cctgggcaac    14548

aatgagaccc ctgtgtctac aaaaagaatt aaaattagcc aggtgtggtg gcacgcacct    14608

gtagtcccag ctacttggga ggctgaggtg ggaggattgc ctgagtccgg gaggcggaag    14668

ttgcaaggag ccatgatcgc gccactgcac ttcaacctag gcaacagagt gagactttgt    14728

ctcaaaaaac aatcatataa taattttaaa ataaatagat ttggcttcct ctaaatgtcc    14788

ccggggactc cgtgcatctt ctgtggagtg tctccgtgag attcgggact cagatcctca    14848

agtgcaactg acccacccga taagctgagg cttcatcatc ccctggccgg tctatgtcga    14908

ctgggcaccc gaggctcctc tcccaccagc tctcttggtc agctgaaagc aaactgttaa    14968

caccctgggg agctggacgt atgagaccct tggggtggga ggcgttgatt tttgagagca    15028

atcacctggc cctggctggc agtaccggga cactgctgtg gctccggggt gggctgtctc    15088

cagaaaatgc ctggcctgag gcagccaccc gcatccagcc cagagggttt attcttgcaa    15148

tgtgctgctg cttcctgccc tgagcacctg gatcccggct tctgccctga ggccccttga    15208

gtcccacagg tagcaagcgc ttgccctgcg gctgctgcat ggggctaact aacgcttcct    15268

caccagtgtc tgctaagtgt ctcctctgtc tcccacgccc tgctctcctg tccccccagt    15328

ttgtctgctg tgaggggaca gaagaggtgt gtgccgcccc cacccctgcc cgggcccttg    15388

ttcctgggat tgctgttttc agctgtttga gctttgatcc tggttctctg gcttcctcaa    15448

agtgagctcg gccagaggag gaaggccatg tgctttctgg ttgaagtcaa gtctggtgcc    15508

ctggtggagg ctgtgctgct gaggcggagc tggggagaga gtgcacacgg gctgcgtggc    15568

caacccctct gggtagctga tgcccaaaga cgctgcagtg cccaggacat ctgggacctc    15628

cctggggccc gcccgtgtgt cccgcgctgt gttcatctgc gggctagcct gtgacccgcg    15688

ctgtgctcgt ctgcgggcta gcctgtgtcc cgcgctctgc ttgtctgcgg tctagcctgt    15748

gacctggcag agagccacca gatgtcccgg gctgagcact gccctctgag caccttcaca    15808

ggaagccctt ctcctggtga gaagagatgc cagcccctgg catctggggg cactggatcc    15868

ctggcctgag ccctagcctc tccccagcct gggggcccct tcccagcagg ctggccctgc    15928

tccttctcta cctgggaccc ttctgcctcc tggctggacc ctggaagctc tgcagggcct    15988

gctgtccccc tccctgccct ccaggtatcc tgaccaccgg ccctggctcc cactgccatc    16048

cactcctctc ctttctggcc gttccctggt ccctgtccca gcccccctcc ccctctcacg    16108

agttacctca cccaggccag agggaagagg gaaggaggcc ctggtcatac cagcacgtcc    16168

tcccacctcc ctcggccctg gtccaccccc tcagtgctgg cctcagagca cagctctctc    16228

caagccaggc cgcgcgccat ccatcctccc tgtcccccaa cgtccttgcc acagatcatg    16288

tccgccctga cacacatggg tctcagccat ctctgcccca gttaactccc catccataaa    16348

gagcacatgc cagccgacac caaaataatt cgggatggtt ccagtttaga cctaagtgga    16408

aggagaaacc accacctgcc ctgcaccttg ttttttggtg accttgataa accatcttca    16468

gccatgaagc cagctgtctc ccaggaagct ccagggcggt gcttcctcgg gagctgactg    16528

ataggtggga ggtggctgcc cccttgcacc ctcaggtgac cccacacaag gccactgctg    16588

gaggccctgg ggactccagg aatgtcaatc agtgacctgc cccccaggcc ccacacagcc    16648

atggctgcat agaggcctgc ctccaaggga cctgtctgtc tgccactgtg gagtccctac    16708

agcgtgcccc ccacagggga gctggttctt tgactgagat cagctggcag ctcagggtca    16768

tcattcccag agggagcggt gccctggagg ccacaggcct cctcatgtgt gtctgcgtcc    16828

gctcgagctt actgagacac taaatctgtt ggtttctgct gtgccaccta cccaccctgt    16888

tggtgttgct ttgttcctat tgctaaagac aggaatgtcc aggacactga gtgtgcaggt    16948

gcctgctggt tctcacgtcc gagctgctga actccgctgg gtcctgctta ctgatggtct    17008

ttgctctagt gctttccagg gtccgtggaa gcttttcctg gaataaagcc cacgcatcga    17068

ccctcacagc gcctcccctc tttgaggccc agcagatacc ccactcctgc ctttccagca    17128

agatttttca gatgctgtgc atactcatca tattgatcac ttttttcttc atgcctgatt    17188

gtgatctgtc aatttcatgt caggaaaggg agtgacattt ttacacttaa gcgtttgctg    17248

agcaaatgtc tgggtcttgc acaatgacaa tgggtccctg tttttcccag aggctctttt    17308

gttctgcagg gattgaagac actccagtcc cacagtcccc agctcccctg gggcagggtt    17368

ggcagaattt cgacaacaca tttttccacc ctgactagga tgtgctcctc atggcagctg    17428

ggaaccactg tccaataagg gcctgggctt acacagctgc ttctcattga gttacaccct    17488

taataaaata atcccatttt atcctttttg tctctctgtc ttcctctctc tctgcctttc    17548

ctcttctctc tcctcctctc tcatctccag                                     17578


<210>  79
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic oligonucleotide

<400>  79
tatctgcacc tttggtag                                                     18


<210>  80
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic oligonucleotide

<400>  80
tgaaggtact cacactgccg c                                                 21


<210>  81
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  81
tgcaaaaacc caaaatattt                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  82
aaaatatttt agctcctact                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  83
cagagtaaca gtctgagtag                                                   20


<210>  84
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  84
taagggatat ttgttcttac                                                   20


<210>  85
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  85
ctaagggata tttgttctta                                                   20


<210>  86
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  86
tgttcttaca ggcaacaatg                                                   20


<210>  87
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  87
tgtatgcttt tctgttaaag                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  88
atgtgtatgc ttttctgtta                                                   20


<210>  89
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  89
gtgtatgctt ttctgttaaa                                                   20


<210>  90
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  90
ttgccttttt ggtatcttac                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  91
tttgcctttt tggtatctta                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  92
cgctgcccaa tgccatcctg                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  93
atttattttt ccttttattc                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  94
tttcctttta ttctagttga                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  95
tgattctgaa ttctttcaac                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  96
atccatatgc ttttacctgc                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  97
gatccatatg cttttacctg                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  98
cagatctgtc aaatcgcctg                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  99
ttattcttct ttctccaggc                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  100
aattttattc ttctttctcc                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  101
caattttatt cttctttctc                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  102
gttttaaaat ttttatatta                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  103
ttttatatta cagaatataa                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  104
atattacaga atataaaaga                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  105
tgtgtatgtg tatgtgtttt                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  106
tatgtgtatg tgttttaggc                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  107
ctattccagt caaataggtc                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  108
gtgtagtgtt aatgtgctta                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  109
ggacttctta tctggatagg                                                   20


<210>  110
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  110
taggtggtat caacatctgt                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  111
tgaaaattta tttccacatg                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  112
gaaaatttat ttccacatgt                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  113
ttacattttt gacctacatg                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  114
aaagaaaatc acagaaacca                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  115
aaaatcacag aaaccaaggt                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  116
ggtatctttg atactaacct                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  117
tatgtgttac ctacccttgt                                                   20


<210>  118
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  118
aaatgtacaa ggaccgacaa                                                   20


<210>  119
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  119
gtacaaggac cgacaagggt                                                   20


<210>  120
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  120
tgcactattc tcaacaggta                                                   20


<210>  121
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  121
tcaaatgcac tattctcaac                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  122
ctttacacac tttacctgtt                                                   20


<210>  123
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  123
atgctctcat ccatagtcat                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  124
tctcatccat agtcataggt                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  125
catccatagt cataggtaag                                                   20


<210>  126
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  126
tgaacatttg gtcctttgca                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  127
tctgaacatt tggtcctttg                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  128
tctcgctcac tcaccctgca                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  129
ggcacagcaa tagatctccg                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  130
taagaactct gaatgtccgc                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  131
gttcttctga tcaggttgaa                                                   20


<210>  132
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  132
tcacgtacct gagagatcct                                                   20


<210>  133
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  133
gaatagccac agggcccgag                                                   20


<210>  134
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  134
tgaagccttg ataaagatac                                                   20


<210>  135
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  135
cagatatgag ggtgggagaa                                                   20


<210>  136
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  136
caggggaatg ggttcctggg                                                   20


<210>  137
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  guide RNA

<400>  137
cccctccctg aactcacact                                                   20


<210>  138
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  138
gtactcacct gccctc                                                       16


<210>  139
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  139
gaacttacct cggcac                                                       16


<210>  140
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  140
ggactcacct agtcag                                                       16


<210>  141
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  141
gcacttacct attggc                                                       16


<210>  142
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  142
gctattacct taaccc                                                       16


<210>  143
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  143
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgagggcagg tgagtacaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  144
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  144
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgtgccgagg taagttcaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  145
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  145
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tctgactagg tgagtccaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  146
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  146
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgccaatagg taagtgcaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  147
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  147
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgggttaagg taatagcaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  148
<211>  247
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence

<400>  148
gtgagtctat gggacccttg atgttctttt aatatacttt tttgtttatc ttatttctaa       60

tactttccct aatctctttc tttcagggca ataatgatac aatgtatcat gcctctttgc      120

accattctaa agaataacag tgataatttc tgggttaagg caatagcaat atttctgcat      180

ataaatattt agtccaagct aggccctttt gctaatcatg ttcatacctc ttatcctcct      240

cccacag                                                                247


<210>  149
<211>  16
<212>  DNA
<213>  Artificial

<220>
<223>  regulatory sequence-binding oligonucleotide

<400>  149
gctattgcct taaccc                                                       16


<210>  150
<211>  1053
<212>  PRT
<213>  Staphylococcus aureus

<400>  150

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  151
<211>  1307
<212>  PRT
<213>  Acidaminococcus fermentans

<400>  151

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  152
<211>  984
<212>  PRT
<213>  Campylobacter jejuni

<400>  152

Met Ala Arg Ile Leu Ala Phe Asp Ile Gly Ile Ser Ser Ile Gly Trp 
1               5                   10                  15      


Ala Phe Ser Glu Asn Asp Glu Leu Lys Asp Cys Gly Val Arg Ile Phe 
            20                  25                  30          


Thr Lys Val Glu Asn Pro Lys Thr Gly Glu Ser Leu Ala Leu Pro Arg 
        35                  40                  45              


Arg Leu Ala Arg Ser Ala Arg Lys Arg Leu Ala Arg Arg Lys Ala Arg 
    50                  55                  60                  


Leu Asn His Leu Lys His Leu Ile Ala Asn Glu Phe Lys Leu Asn Tyr 
65                  70                  75                  80  


Glu Asp Tyr Gln Ser Phe Asp Glu Ser Leu Ala Lys Ala Tyr Lys Gly 
                85                  90                  95      


Ser Leu Ile Ser Pro Tyr Glu Leu Arg Phe Arg Ala Leu Asn Glu Leu 
            100                 105                 110         


Leu Ser Lys Gln Asp Phe Ala Arg Val Ile Leu His Ile Ala Lys Arg 
        115                 120                 125             


Arg Gly Tyr Asp Asp Ile Lys Asn Ser Asp Asp Lys Glu Lys Gly Ala 
    130                 135                 140                 


Ile Leu Lys Ala Ile Lys Gln Asn Glu Glu Lys Leu Ala Asn Tyr Gln 
145                 150                 155                 160 


Ser Val Gly Glu Tyr Leu Tyr Lys Glu Tyr Phe Gln Lys Phe Lys Glu 
                165                 170                 175     


Asn Ser Lys Glu Phe Thr Asn Val Arg Asn Lys Lys Glu Ser Tyr Glu 
            180                 185                 190         


Arg Cys Ile Ala Gln Ser Phe Leu Lys Asp Glu Leu Lys Leu Ile Phe 
        195                 200                 205             


Lys Lys Gln Arg Glu Phe Gly Phe Ser Phe Ser Lys Lys Phe Glu Glu 
    210                 215                 220                 


Glu Val Leu Ser Val Ala Phe Tyr Lys Arg Ala Leu Lys Asp Phe Ser 
225                 230                 235                 240 


His Leu Val Gly Asn Cys Ser Phe Phe Thr Asp Glu Lys Arg Ala Pro 
                245                 250                 255     


Lys Asn Ser Pro Leu Ala Phe Met Phe Val Ala Leu Thr Arg Ile Ile 
            260                 265                 270         


Asn Leu Leu Asn Asn Leu Lys Asn Thr Glu Gly Ile Leu Tyr Thr Lys 
        275                 280                 285             


Asp Asp Leu Asn Ala Leu Leu Asn Glu Val Leu Lys Asn Gly Thr Leu 
    290                 295                 300                 


Thr Tyr Lys Gln Thr Lys Lys Leu Leu Gly Leu Ser Asp Asp Tyr Glu 
305                 310                 315                 320 


Phe Lys Gly Glu Lys Gly Thr Tyr Phe Ile Glu Phe Lys Lys Tyr Lys 
                325                 330                 335     


Glu Phe Ile Lys Ala Leu Gly Glu His Asn Leu Ser Gln Asp Asp Leu 
            340                 345                 350         


Asn Glu Ile Ala Lys Asp Ile Thr Leu Ile Lys Asp Glu Ile Lys Leu 
        355                 360                 365             


Lys Lys Ala Leu Ala Lys Tyr Asp Leu Asn Gln Asn Gln Ile Asp Ser 
    370                 375                 380                 


Leu Ser Lys Leu Glu Phe Lys Asp His Leu Asn Ile Ser Phe Lys Ala 
385                 390                 395                 400 


Leu Lys Leu Val Thr Pro Leu Met Leu Glu Gly Lys Lys Tyr Asp Glu 
                405                 410                 415     


Ala Cys Asn Glu Leu Asn Leu Lys Val Ala Ile Asn Glu Asp Lys Lys 
            420                 425                 430         


Asp Phe Leu Pro Ala Phe Asn Glu Thr Tyr Tyr Lys Asp Glu Val Thr 
        435                 440                 445             


Asn Pro Val Val Leu Arg Ala Ile Lys Glu Tyr Arg Lys Val Leu Asn 
    450                 455                 460                 


Ala Leu Leu Lys Lys Tyr Gly Lys Val His Lys Ile Asn Ile Glu Leu 
465                 470                 475                 480 


Ala Arg Glu Val Gly Lys Asn His Ser Gln Arg Ala Lys Ile Glu Lys 
                485                 490                 495     


Glu Gln Asn Glu Asn Tyr Lys Ala Lys Lys Asp Ala Glu Leu Glu Cys 
            500                 505                 510         


Glu Lys Leu Gly Leu Lys Ile Asn Ser Lys Asn Ile Leu Lys Leu Arg 
        515                 520                 525             


Leu Phe Lys Glu Gln Lys Glu Phe Cys Ala Tyr Ser Gly Glu Lys Ile 
    530                 535                 540                 


Lys Ile Ser Asp Leu Gln Asp Glu Lys Met Leu Glu Ile Asp His Ile 
545                 550                 555                 560 


Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr Met Asn Lys Val Leu 
                565                 570                 575     


Val Phe Thr Lys Gln Asn Gln Glu Lys Leu Asn Gln Thr Pro Phe Glu 
            580                 585                 590         


Ala Phe Gly Asn Asp Ser Ala Lys Trp Gln Lys Ile Glu Val Leu Ala 
        595                 600                 605             


Lys Asn Leu Pro Thr Lys Lys Gln Lys Arg Ile Leu Asp Lys Asn Tyr 
    610                 615                 620                 


Lys Asp Lys Glu Gln Lys Asn Phe Lys Asp Arg Asn Leu Asn Asp Thr 
625                 630                 635                 640 


Arg Tyr Ile Ala Arg Leu Val Leu Asn Tyr Thr Lys Asp Tyr Leu Asp 
                645                 650                 655     


Phe Leu Pro Leu Ser Asp Asp Glu Asn Thr Lys Leu Asn Asp Thr Gln 
            660                 665                 670         


Lys Gly Ser Lys Val His Val Glu Ala Lys Ser Gly Met Leu Thr Ser 
        675                 680                 685             


Ala Leu Arg His Thr Trp Gly Phe Ser Ala Lys Asp Arg Asn Asn His 
    690                 695                 700                 


Leu His His Ala Ile Asp Ala Val Ile Ile Ala Tyr Ala Asn Asn Ser 
705                 710                 715                 720 


Ile Val Lys Ala Phe Ser Asp Phe Lys Lys Glu Gln Glu Ser Asn Ser 
                725                 730                 735     


Ala Glu Leu Tyr Ala Lys Lys Ile Ser Glu Leu Asp Tyr Lys Asn Lys 
            740                 745                 750         


Arg Lys Phe Phe Glu Pro Phe Ser Gly Phe Arg Gln Lys Val Leu Asp 
        755                 760                 765             


Lys Ile Asp Glu Ile Phe Val Ser Lys Pro Glu Arg Lys Lys Pro Ser 
    770                 775                 780                 


Gly Ala Leu His Glu Glu Thr Phe Arg Lys Glu Glu Glu Phe Tyr Gln 
785                 790                 795                 800 


Ser Tyr Gly Gly Lys Glu Gly Val Leu Lys Ala Leu Glu Leu Gly Lys 
                805                 810                 815     


Ile Arg Lys Val Asn Gly Lys Ile Val Lys Asn Gly Asp Met Phe Arg 
            820                 825                 830         


Val Asp Ile Phe Lys His Lys Lys Thr Asn Lys Phe Tyr Ala Val Pro 
        835                 840                 845             


Ile Tyr Thr Met Asp Phe Ala Leu Lys Val Leu Pro Asn Lys Ala Val 
    850                 855                 860                 


Ala Arg Ser Lys Lys Gly Glu Ile Lys Asp Trp Ile Leu Met Asp Glu 
865                 870                 875                 880 


Asn Tyr Glu Phe Cys Phe Ser Leu Tyr Lys Asp Ser Leu Ile Leu Ile 
                885                 890                 895     


Gln Thr Lys Asp Met Gln Glu Pro Glu Phe Val Tyr Tyr Asn Ala Phe 
            900                 905                 910         


Thr Ser Ser Thr Val Ser Leu Ile Val Ser Lys His Asp Asn Lys Phe 
        915                 920                 925             


Glu Thr Leu Ser Lys Asn Gln Lys Ile Leu Phe Lys Asn Ala Asn Glu 
    930                 935                 940                 


Lys Glu Val Ile Ala Lys Ser Ile Gly Ile Gln Asn Leu Lys Val Phe 
945                 950                 955                 960 


Glu Lys Tyr Ile Val Ser Ala Leu Gly Glu Val Thr Lys Ala Glu Phe 
                965                 970                 975     


Arg Gln Arg Glu Asp Phe Lys Lys 
            980                 


<210>  153
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  structural motif

<400>  153

Leu Ala Gly Leu Ile Asp Ala Asp Gly 
1               5                   


<210>  154
<211>  887
<212>  PRT
<213>  Natronobacterium gregoryi

<400>  154

Met Thr Val Ile Asp Leu Asp Ser Thr Thr Thr Ala Asp Glu Leu Thr 
1               5                   10                  15      


Ser Gly His Thr Tyr Asp Ile Ser Val Thr Leu Thr Gly Val Tyr Asp 
            20                  25                  30          


Asn Thr Asp Glu Gln His Pro Arg Met Ser Leu Ala Phe Glu Gln Asp 
        35                  40                  45              


Asn Gly Glu Arg Arg Tyr Ile Thr Leu Trp Lys Asn Thr Thr Pro Lys 
    50                  55                  60                  


Asp Val Phe Thr Tyr Asp Tyr Ala Thr Gly Ser Thr Tyr Ile Phe Thr 
65                  70                  75                  80  


Asn Ile Asp Tyr Glu Val Lys Asp Gly Tyr Glu Asn Leu Thr Ala Thr 
                85                  90                  95      


Tyr Gln Thr Thr Val Glu Asn Ala Thr Ala Gln Glu Val Gly Thr Thr 
            100                 105                 110         


Asp Glu Asp Glu Thr Phe Ala Gly Gly Glu Pro Leu Asp His His Leu 
        115                 120                 125             


Asp Asp Ala Leu Asn Glu Thr Pro Asp Asp Ala Glu Thr Glu Ser Asp 
    130                 135                 140                 


Ser Gly His Val Met Thr Ser Phe Ala Ser Arg Asp Gln Leu Pro Glu 
145                 150                 155                 160 


Trp Thr Leu His Thr Tyr Thr Leu Thr Ala Thr Asp Gly Ala Lys Thr 
                165                 170                 175     


Asp Thr Glu Tyr Ala Arg Arg Thr Leu Ala Tyr Thr Val Arg Gln Glu 
            180                 185                 190         


Leu Tyr Thr Asp His Asp Ala Ala Pro Val Ala Thr Asp Gly Leu Met 
        195                 200                 205             


Leu Leu Thr Pro Glu Pro Leu Gly Glu Thr Pro Leu Asp Leu Asp Cys 
    210                 215                 220                 


Gly Val Arg Val Glu Ala Asp Glu Thr Arg Thr Leu Asp Tyr Thr Thr 
225                 230                 235                 240 


Ala Lys Asp Arg Leu Leu Ala Arg Glu Leu Val Glu Glu Gly Leu Lys 
                245                 250                 255     


Arg Ser Leu Trp Asp Asp Tyr Leu Val Arg Gly Ile Asp Glu Val Leu 
            260                 265                 270         


Ser Lys Glu Pro Val Leu Thr Cys Asp Glu Phe Asp Leu His Glu Arg 
        275                 280                 285             


Tyr Asp Leu Ser Val Glu Val Gly His Ser Gly Arg Ala Tyr Leu His 
    290                 295                 300                 


Ile Asn Phe Arg His Arg Phe Val Pro Lys Leu Thr Leu Ala Asp Ile 
305                 310                 315                 320 


Asp Asp Asp Asn Ile Tyr Pro Gly Leu Arg Val Lys Thr Thr Tyr Arg 
                325                 330                 335     


Pro Arg Arg Gly His Ile Val Trp Gly Leu Arg Asp Glu Cys Ala Thr 
            340                 345                 350         


Asp Ser Leu Asn Thr Leu Gly Asn Gln Ser Val Val Ala Tyr His Arg 
        355                 360                 365             


Asn Asn Gln Thr Pro Ile Asn Thr Asp Leu Leu Asp Ala Ile Glu Ala 
    370                 375                 380                 


Ala Asp Arg Arg Val Val Glu Thr Arg Arg Gln Gly His Gly Asp Asp 
385                 390                 395                 400 


Ala Val Ser Phe Pro Gln Glu Leu Leu Ala Val Glu Pro Asn Thr His 
                405                 410                 415     


Gln Ile Lys Gln Phe Ala Ser Asp Gly Phe His Gln Gln Ala Arg Ser 
            420                 425                 430         


Lys Thr Arg Leu Ser Ala Ser Arg Cys Ser Glu Lys Ala Gln Ala Phe 
        435                 440                 445             


Ala Glu Arg Leu Asp Pro Val Arg Leu Asn Gly Ser Thr Val Glu Phe 
    450                 455                 460                 


Ser Ser Glu Phe Phe Thr Gly Asn Asn Glu Gln Gln Leu Arg Leu Leu 
465                 470                 475                 480 


Tyr Glu Asn Gly Glu Ser Val Leu Thr Phe Arg Asp Gly Ala Arg Gly 
                485                 490                 495     


Ala His Pro Asp Glu Thr Phe Ser Lys Gly Ile Val Asn Pro Pro Glu 
            500                 505                 510         


Ser Phe Glu Val Ala Val Val Leu Pro Glu Gln Gln Ala Asp Thr Cys 
        515                 520                 525             


Lys Ala Gln Trp Asp Thr Met Ala Asp Leu Leu Asn Gln Ala Gly Ala 
    530                 535                 540                 


Pro Pro Thr Arg Ser Glu Thr Val Gln Tyr Asp Ala Phe Ser Ser Pro 
545                 550                 555                 560 


Glu Ser Ile Ser Leu Asn Val Ala Gly Ala Ile Asp Pro Ser Glu Val 
                565                 570                 575     


Asp Ala Ala Phe Val Val Leu Pro Pro Asp Gln Glu Gly Phe Ala Asp 
            580                 585                 590         


Leu Ala Ser Pro Thr Glu Thr Tyr Asp Glu Leu Lys Lys Ala Leu Ala 
        595                 600                 605             


Asn Met Gly Ile Tyr Ser Gln Met Ala Tyr Phe Asp Arg Phe Arg Asp 
    610                 615                 620                 


Ala Lys Ile Phe Tyr Thr Arg Asn Val Ala Leu Gly Leu Leu Ala Ala 
625                 630                 635                 640 


Ala Gly Gly Val Ala Phe Thr Thr Glu His Ala Met Pro Gly Asp Ala 
                645                 650                 655     


Asp Met Phe Ile Gly Ile Asp Val Ser Arg Ser Tyr Pro Glu Asp Gly 
            660                 665                 670         


Ala Ser Gly Gln Ile Asn Ile Ala Ala Thr Ala Thr Ala Val Tyr Lys 
        675                 680                 685             


Asp Gly Thr Ile Leu Gly His Ser Ser Thr Arg Pro Gln Leu Gly Glu 
    690                 695                 700                 


Lys Leu Gln Ser Thr Asp Val Arg Asp Ile Met Lys Asn Ala Ile Leu 
705                 710                 715                 720 


Gly Tyr Gln Gln Val Thr Gly Glu Ser Pro Thr His Ile Val Ile His 
                725                 730                 735     


Arg Asp Gly Phe Met Asn Glu Asp Leu Asp Pro Ala Thr Glu Phe Leu 
            740                 745                 750         


Asn Glu Gln Gly Val Glu Tyr Asp Ile Val Glu Ile Arg Lys Gln Pro 
        755                 760                 765             


Gln Thr Arg Leu Leu Ala Val Ser Asp Val Gln Tyr Asp Thr Pro Val 
    770                 775                 780                 


Lys Ser Ile Ala Ala Ile Asn Gln Asn Glu Pro Arg Ala Thr Val Ala 
785                 790                 795                 800 


Thr Phe Gly Ala Pro Glu Tyr Leu Ala Thr Arg Asp Gly Gly Gly Leu 
                805                 810                 815     


Pro Arg Pro Ile Gln Ile Glu Arg Val Ala Gly Glu Thr Asp Ile Glu 
            820                 825                 830         


Thr Leu Thr Arg Gln Val Tyr Leu Leu Ser Gln Ser His Ile Gln Val 
        835                 840                 845             


His Asn Ser Thr Ala Arg Leu Pro Ile Thr Thr Ala Tyr Ala Asp Gln 
    850                 855                 860                 


Ala Ser Thr His Ala Thr Lys Gly Tyr Leu Val Gln Thr Gly Ala Phe 
865                 870                 875                 880 


Glu Ser Asn Val Gly Phe Leu 
                885         


