                         SEQUENCE LISTING

<110>  University of Pretoria
       CSIR
 
<120>  Plant Produced Avian Influenza Antigens and their Uses in 
       Diagnostic Assays and Devices

<130>  PA168743/P

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  365
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Full-length H7-HA1 construct

<400>  1

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly Asp Lys Ile Cys Leu Gly His His 
            20                  25                  30          


Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val 
        35                  40                  45              


Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Thr Asn Val Pro Arg 
    50                  55                  60                  


Ile Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu 
65                  70                  75                  80  


Leu Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe 
                85                  90                  95      


Ser Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr 
            100                 105                 110         


Pro Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu 
        115                 120                 125             


Ser Gly Gly Ile Asn Lys Glu Thr Met Gly Phe Thr Tyr Ser Gly Ile 
    130                 135                 140                 


Arg Thr Asn Gly Ala Thr Ser Ala Cys Lys Arg Ser Gly Ser Ser Phe 
145                 150                 155                 160 


Tyr Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe 
                165                 170                 175     


Pro Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Asp Pro Ala Leu 
            180                 185                 190         


Ile Ile Trp Gly Ile His His Ser Gly Ser Thr Thr Glu Gln Thr Lys 
        195                 200                 205             


Leu Tyr Gly Ser Gly Ser Lys Leu Ile Thr Val Gly Ser Ser Asn Tyr 
    210                 215                 220                 


Gln Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly 
225                 230                 235                 240 


Gln Ser Gly Arg Ile Asp Phe His Trp Leu Met Leu Asn Pro Asn Asp 
                245                 250                 255     


Thr Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala 
            260                 265                 270         


Ser Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Gly Gly Val Gln Val 
        275                 280                 285             


Asp Ala Ser Cys Glu Gly Asp Cys Tyr His Ser Gly Gly Thr Ile Ile 
    290                 295                 300                 


Ser Asn Leu Pro Phe Gln Asn Ile Asn Ser Arg Ala Val Gly Lys Cys 
305                 310                 315                 320 


Pro Arg Tyr Val Lys Gln Glu Ser Leu Met Leu Ala Thr Gly Met Lys 
                325                 330                 335     


Asn Val Pro Glu Leu Pro Lys Gly Arg Gly Leu Phe Leu Val Pro Arg 
            340                 345                 350         


Gly Ser Ser Gly His His His His His His Asp Glu Leu 
        355                 360                 365 


<210>  2
<211>  1098
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full-length H7-HA1 construct

<400>  2
atggcaaaca agcacctaag tctgagtctg ttcctggtat tgctgggact ttcagcttct       60

ttggcaagtg gtgataagat ctgtctaggt catcatgctg taagtaacgg aacaaaggta      120

aacacgctaa cggagagagg agttgaagtt gttaacgcaa cagagacggt tgaaagaacc      180

aacgttccaa gaatctgtag taagggaaag aggaccgttg atctcggaca atgtggattg      240

ttgggaacta tcacaggacc accacaatgt gatcaattcc tcgaattctc cgctgatttg      300

atcatcgaac gacgagaagg atcagatgtt tgttaccctg gtaagttcgt taacgaggaa      360

gcactcagac aaattctcag agaatccggt ggtattaaca aggagaccat gggtttcact      420

tactccggta ttagaaccaa tggtgcaacc tcagcttgca aacgtagtgg ttcatctttc      480

tacgcagaaa tgaagtggtt gctttcaaat actgacaatg ccgcttttcc tcagatgact      540

aagtcctaca agaatacacg gaaggaccct gctttgataa tttggggtat acaccactcc      600

ggatcaacaa cagagcagac taaactttat ggtagcggta gcaaacttat tactgtcggt      660

agcagcaatt atcagcagag ctttgtccct tctccgggag caaggccaca agttaatgga      720

cagtctggtc gtattgattt tcattggctt atgcttaatc ctaatgacac agtcactttt      780

tcttttaatg gcgcctttat tgccccggac agggcctctt ttcttagggg caaatctatg      840

ggcattcagg ggggggtgca agtggatgct tcttgcgagg gggattgcta tcattctggc      900

ggcactataa tttctaattt accatttcaa aatataaatt cacgggctgt ggggaaatgc      960

cctaggtatg tgaaacagga gtcacttatg ttagctacag ggatgaaaaa tgtgcccgag     1020

ttacccaaag gccgtgggtt atttttagtt ccgcggggtt catctgggca tcatcaccat     1080

caccacgatg agttatga                                                   1098


<210>  3
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated H7-HA1 construct

<400>  3

Met Ala Asn Lys His Leu Ser Leu Ser Leu Phe Leu Val Leu Leu Gly 
1               5                   10                  15      


Leu Ser Ala Ser Leu Ala Ser Gly Asp Lys Ile Cys Leu Gly His His 
            20                  25                  30          


Ala Val Ser Asn Gly Thr Lys Val Asn Thr Leu Thr Glu Arg Gly Val 
        35                  40                  45              


Glu Val Val Asn Ala Thr Glu Thr Val Glu Arg Thr Asn Val Pro Arg 
    50                  55                  60                  


Ile Cys Ser Lys Gly Lys Arg Thr Val Asp Leu Gly Gln Cys Gly Leu 
65                  70                  75                  80  


Leu Gly Thr Ile Thr Gly Pro Pro Gln Cys Asp Gln Phe Leu Glu Phe 
                85                  90                  95      


Ser Ala Asp Leu Ile Ile Glu Arg Arg Glu Gly Ser Asp Val Cys Tyr 
            100                 105                 110         


Pro Gly Lys Phe Val Asn Glu Glu Ala Leu Arg Gln Ile Leu Arg Glu 
        115                 120                 125             


Ser Gly Gly Ile Asn Lys Glu Thr Met Gly Phe Thr Tyr Ser Gly Ile 
    130                 135                 140                 


Arg Thr Asn Gly Ala Thr Ser Ala Cys Lys Arg Ser Gly Ser Ser Phe 
145                 150                 155                 160 


Tyr Ala Glu Met Lys Trp Leu Leu Ser Asn Thr Asp Asn Ala Ala Phe 
                165                 170                 175     


Pro Gln Met Thr Lys Ser Tyr Lys Asn Thr Arg Lys Asp Pro Ala Leu 
            180                 185                 190         


Ile Ile Trp Gly Ile His His Ser Gly Ser Thr Thr Glu Gln Thr Lys 
        195                 200                 205             


Leu Tyr Gly Ser Gly Ser Lys Leu Ile Thr Val Gly Ser Ser Asn Tyr 
    210                 215                 220                 


Gln Gln Ser Phe Val Pro Ser Pro Gly Ala Arg Pro Gln Val Asn Gly 
225                 230                 235                 240 


Gln Ser Gly Arg Ile Asp Phe His Trp Leu Met Leu Asn Pro Asn Asp 
                245                 250                 255     


Thr Val Thr Phe Ser Phe Asn Gly Ala Phe Ile Ala Pro Asp Arg Ala 
            260                 265                 270         


Ser Phe Leu Arg Gly Lys Ser Met Gly Ile Gln Gly Gly Val Gln Val 
        275                 280                 285             


Asp Ala Ser Cys Glu Gly Asp Cys Tyr His Ser Gly Gly Thr Ile Ile 
    290                 295                 300                 


Ser Asn Leu Pro Leu Val Pro Arg Gly Ser Ser Gly His His His His 
305                 310                 315                 320 


His His Asp Glu Leu 
                325 


<210>  4
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated H7-HA1 construct

<400>  4
atggcaaaca agcacctaag tctgagtctg ttcctggtat tgctgggact ttcagcttct       60

ttggcaagtg gtgataagat ctgtctaggt catcatgctg taagtaacgg aacaaaggta      120

aacacgctaa cggagagagg agttgaagtt gttaacgcaa cagagacggt tgaaagaacc      180

aacgttccaa gaatctgtag taagggaaag aggaccgttg atctcggaca atgtggattg      240

ttgggaacta tcacaggacc accacaatgt gatcaattcc tcgaattctc cgctgatttg      300

atcatcgaac gacgagaagg atcagatgtt tgttaccctg gtaagttcgt taacgaggaa      360

gcactcagac aaattctcag agaatccggt ggtattaaca aggagaccat gggtttcact      420

tactccggta ttagaaccaa tggtgcaacc tcagcttgca aacgtagtgg ttcatctttc      480

tacgcagaaa tgaagtggtt gctttcaaat actgacaatg ccgcttttcc tcagatgact      540

aagtcctaca agaatacacg gaaggaccct gctttgataa tttggggtat acaccactcc      600

ggatcaacaa cagagcagac taaactttat ggtagcggta gcaaacttat tactgtcggt      660

agcagcaatt atcagcagag ctttgtccct tctccgggag caaggccaca agttaatgga      720

cagtctggtc gtattgattt tcattggctt atgcttaatc ctaatgacac agtcactttt      780

tcttttaatg gcgcctttat tgccccggac agggcctctt ttcttagggg caaatctatg      840

ggcattcagg ggggggtgca agtggatgct tcttgcgagg gggattgcta tcattctggc      900

ggcactataa tttctaattt accattagtt ccgcggggtt catctgggca tcatcaccat      960

caccacgatg agttatga                                                    978


<210>  5
<211>  356
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated H5-HA1 construct

<400>  5

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly 
1               5                   10                  15      


Val His Cys Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr 
            20                  25                  30          


Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val Thr Val Thr His Ala 
        35                  40                  45              


Gln Asp Ile Leu Glu Lys Ala His Asn Gly Lys Leu Cys Ser Leu Asn 
    50                  55                  60                  


Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser Val Ala Gly Trp Leu 
65                  70                  75                  80  


Leu Gly Asn Pro Met Cys Asp Glu Phe Leu Asn Val Pro Glu Trp Ser 
                85                  90                  95      


Tyr Ile Val Glu Lys Asp Asn Pro Val Asn Gly Leu Cys Tyr Pro Gly 
            100                 105                 110         


Asp Phe Asn Asp Tyr Glu Glu Leu Lys His Leu Leu Ser Ser Thr Asn 
        115                 120                 125             


His Phe Glu Lys Ile Gln Ile Ile Pro Arg Ser Ser Trp Ser Asn His 
    130                 135                 140                 


Asp Ala Ser Ser Gly Val Ser Ser Ala Cys Pro Tyr Asn Gly Arg Ser 
145                 150                 155                 160 


Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys Lys Asn Ser Ala Tyr 
                165                 170                 175     


Pro Thr Ile Lys Arg Ser Tyr Asn Asn Thr Asn Gln Glu Asp Leu Leu 
            180                 185                 190         


Val Leu Trp Gly Ile His His Pro Asn Asp Ala Ala Glu Gln Thr Lys 
        195                 200                 205             


Leu Tyr Gln Asn Pro Thr Thr Tyr Val Ser Val Gly Thr Ser Thr Leu 
    210                 215                 220                 


Asn Gln Arg Ser Val Pro Glu Ile Ala Thr Arg Pro Lys Val Asn Gly 
225                 230                 235                 240 


Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile Leu Lys Pro Asn Asp 
                245                 250                 255     


Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala Pro Glu Tyr Ala 
            260                 265                 270         


Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Val Met Lys Ser Gly Leu 
        275                 280                 285             


Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr Pro Met Gly Ala Ile 
    290                 295                 300                 


Asn Ser Ser Met Pro Phe His Asn Ile His Pro Leu Thr Ile Gly Glu 
305                 310                 315                 320 


Cys Pro Lys Tyr Val Lys Ser Asp Arg Leu Val Leu Ala Thr Gly Leu 
                325                 330                 335     


Arg Asn Val Leu Val Pro Arg Gly Ser Ser Gly His His His His His 
            340                 345                 350         


His Asp Glu Leu 
        355     


<210>  6
<211>  1070
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated H5-HA1 construct

<400>  6
atgggatgga gttggatatt cctgttccta ctatccggtg cagctggagt acattgtgat       60

caaatatgta tcggatacca tgctaataac tccactgaac aggtagatac gatcatggaa      120

aagaacgtaa cggtcactca tgcacaagat atcctggaaa aggcacataa cggtaagcta      180

tgttctctga acggtgttaa gccacttatc ttgagagatt gttccgtagc aggttggctt      240

cttggaaatc ctatgtgtga tgaattctta aacgtccctg aatggtctta catcgttgaa      300

aaggataacc ctgttaacgg actgtgttac ccaggtgatt tcaacgatta cgaagaactc      360

aagcatctcc tctcttctac aaaccatttc gaaaagatac agattattcc ccggagttcc      420

tggtctaacc atgatgcaag tagcggagtt tcaagtgcat gcccttataa tggaagaagt      480

tctttctttc gaaacgtggt ttggctcatt aagaagaata gcgcttaccc aacaattaag      540

cgtagctaca ataataccaa tcaggaggac ttgcttgtct tgtggggaat tcaccaccca      600

aatgacgctg ctgaacaaac taagttgtat cagaatccga ccacttatgt gagcgttggt      660

acatcaactt tgaatcagag aagcgttcct gagattgcta cacgaccaaa agttaatggt      720

caatcaggaa gaatggagtt tttttggacc attcttaagc ccaatgacgc cattaatttt      780

gagtcaaatg ggaattttat tgccccagag tatgcttata agatagtgaa aaaaggcgac      840

agtgccgtga tgaaatcagg cttggagtat ggtaattgca ataccaaatg ccaaacacct      900

atgggcgcca ttaattcttc aatgccgttt cacaatatac accctttgac tattggggag      960

tgcccaaaat atgtcaaatc tgaccgtctt gtgcttgcta cagggcttag gaatgtgtta     1020

gttccgcggg gttcatctgg gcatcatcac catcaccacg atgagttatg                1070


<210>  7
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated H6-HA1 construct

<400>  7

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly 
1               5                   10                  15      


Val His Cys Asp Lys Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr 
            20                  25                  30          


Thr Gln Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser 
        35                  40                  45              


Ile Glu Leu Leu Glu Thr Gln Lys Glu Glu Arg Phe Cys Lys Ile Leu 
    50                  55                  60                  


Asn Lys Ala Pro Leu Asp Leu Gly Glu Cys Thr Ile Glu Gly Trp Ile 
65                  70                  75                  80  


Leu Gly Asn Pro Gln Cys Asp Leu Leu Leu Gly Asp Gln Ser Trp Ser 
                85                  90                  95      


Tyr Ile Val Glu Arg Pro Thr Ala Arg Asn Gly Ile Cys Tyr Pro Gly 
            100                 105                 110         


Val Leu Asn Glu Val Glu Glu Leu Lys Ala Leu Ile Gly Ser Gly Glu 
        115                 120                 125             


Lys Val Glu Arg Phe Glu Met Phe Pro Arg Asn Thr Trp Arg Gly Val 
    130                 135                 140                 


Asp Thr Asn Ser Gly Val Thr Lys Ala Cys Pro Ser Ser Thr Gly Gly 
145                 150                 155                 160 


Ser Ser Phe Tyr Arg Asn Leu Leu Trp Ile Ile Lys Ser Lys Ser Ala 
                165                 170                 175     


Ala Tyr Pro Val Ile Lys Gly Thr Tyr Asn Asn Thr Gly Asn Gln Pro 
            180                 185                 190         


Ile Leu Tyr Phe Trp Gly Val His His Pro Pro Asp Thr Asn Glu Gln 
        195                 200                 205             


Asn Thr Leu Tyr Gly Ser Gly Asp Arg Tyr Val Arg Met Gly Thr Glu 
    210                 215                 220                 


Ser Met Asn Phe Ala Lys Gly Pro Glu Ile Ala Ala Arg Pro Ala Val 
225                 230                 235                 240 


Asn Gly Gln Arg Gly Arg Ile Asp Tyr Tyr Trp Ser Val Leu Lys Pro 
                245                 250                 255     


Gly Glu Thr Leu Asn Val Glu Ser Asn Gly Asn Leu Ile Ala Pro Trp 
            260                 265                 270         


Tyr Ala Tyr Lys Phe Val Ser Thr Ser Asn Lys Gly Ala Val Phe Lys 
        275                 280                 285             


Ser Asn Leu Pro Val Glu Asp Cys His Ala Ile Cys Gln Thr Ala Ala 
    290                 295                 300                 


Gly Val Leu Arg Leu Val Pro Arg Gly Ser Ser Gly His His His His 
305                 310                 315                 320 


His His Asp Glu Leu 
                325 


<210>  8
<211>  978
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated H6-HA1 construct

<400>  8
atgggatgga gctggatctt cctgttcctg ctgagcggcg ctgctggagt acactgtgat       60

aaaatctgca tcggatacca cgctaacaat agcacaaccc aggtggatac aatcctggag      120

aagaacgtga cagtgactca ctccatcgag ctgctggaaa ctcagaaaga ggaaagattt      180

tgcaagattc tgaacaaggc tccactggat ctgggagagt gcacaatcga aggatggatt      240

ctgggaaatc ctcagtgtga tctgctgctg ggagatcaga gctggagcta catcgtggag      300

agacccaccg ccagaaacgg catctgctac cccggcgtgc tgaacgaggt ggaggagctg      360

aaggccctga tcggcagcgg cgagaaggtg gagagattcg agatgttccc taggaacaca      420

tggaggggcg tggacacaaa cagcggcgtg acaaaggcct gccccagcag caccggcggc      480

agcagcttct acagaaacct gctgtggatc ataaagagca agagcgccgc ctaccccgtg      540

atcaagggca cctacaacaa caccggcaac cagcccatcc tgtacttctg gggcgtgcac      600

cacccccccg acaccaacga gcagaacaca ctgtacggaa gcggcgatag gtacgtgaga      660

atgggcactg aatccatgaa ttttgctaaa ggacctgaga tagctgctag acccgccgtt      720

aacggccaga gaggcagaat cgattactac tggagcgtgc tgaagcccgg cgagacactg      780

aacgtggagt ctaacggcaa cctgatcgcc ccttggtacg cctacaagtt cgtgagcacc      840

agcaacaagg gagccgtgtt caagagcaac ctgcccgtgg aggattgcca cgccatctgc      900

cagaccgccg ccggcgtgct gagactggtg cccaggggca gcagcggcca ccaccaccac      960

catcatgatg aactgtga                                                    978


<210>  9
<211>  313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated H9-HA1 construct

<400>  9

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly 
1               5                   10                  15      


Val His Cys Asp Lys Ile Cys Val Gly His Gln Ser Thr Asn Ser Thr 
            20                  25                  30          


Glu Thr Val Asp Thr Leu Thr Glu Thr Asn Val Pro Val Thr Gln Ala 
        35                  40                  45              


Lys Glu Leu Leu His Thr Glu His Asn Gly Met Leu Cys Ala Thr Asn 
    50                  55                  60                  


Leu Gly Arg Pro Leu Ile Leu Asp Thr Cys Thr Ile Glu Gly Leu Ile 
65                  70                  75                  80  


Tyr Gly Asn Pro Ser Cys Asp Met Leu Leu Gly Gly Arg Glu Trp Ser 
                85                  90                  95      


Tyr Ile Val Glu Arg Pro Ser Ala Val Asn Gly Thr Cys Tyr Pro Gly 
            100                 105                 110         


Asn Ile Glu Asn Leu Glu Glu Leu Arg Thr Leu Phe Ser Ser Ser Ser 
        115                 120                 125             


Ser Tyr Gln Arg Ile Gln Leu Phe Pro Asp Thr Ile Trp Asn Val Thr 
    130                 135                 140                 


Tyr Thr Gly Thr Ser Lys Ser Cys Ser Asp Ser Phe Tyr Arg Asn Met 
145                 150                 155                 160 


Arg Trp Leu Thr Gln Lys Asn Gly Leu Tyr Pro Ile Gln Asp Ala Gln 
                165                 170                 175     


Tyr Thr Asn Asn Arg Gly Lys Asp Ile Leu Phe Val Trp Gly Ile His 
            180                 185                 190         


His Pro Pro Thr Asp Thr Ala Gln Thr Asn Leu Tyr Thr Arg Thr Asp 
        195                 200                 205             


Thr Thr Thr Ser Val Thr Thr Glu Asn Leu Asp Arg Thr Phe Lys Pro 
    210                 215                 220                 


Leu Ile Gly Pro Arg Pro Leu Val Asn Gly Leu Ile Gly Arg Ile Asn 
225                 230                 235                 240 


Tyr Tyr Trp Ser Val Leu Lys Pro Gly Gln Thr Leu Arg Val Arg Ser 
                245                 250                 255     


Asn Gly Asn Leu Ile Ala Pro Trp Phe Gly His Ile Leu Ser Gly Glu 
            260                 265                 270         


Ser His Gly Arg Ile Leu Arg Thr Asp Leu Ser Ser Gly Asn Cys Val 
        275                 280                 285             


Val Gln Cys Gln Thr Glu Lys Gly Leu Val Pro Arg Gly Ser Ser Gly 
    290                 295                 300                 


His His His His His His Asp Glu Leu 
305                 310             


<210>  10
<211>  960
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Truncated H9-HA1 construct

<400>  10
gacaccggta tgggctggag ctggatcttt ctgttcctgc tgagcggcgc cgcaggagtg       60

cactgcgata aaatctgcgt gggacaccag agcacaaaca gcacagagac agtggataca      120

ctgacagaga ctaacgtgcc tgtgacacag gccaaggaac tgctgcacac agagcacaac      180

ggcatgctgt gcgcaacaaa cctgggcaga ccactgattc ttgatacatg cacaattgaa      240

ggtctgatct acggaaaccc tagctgcgat atgctgctgg gaggaaggga gtggtcatac      300

attgtggaaa gacctagcgc cgtgaacggc acttgctacc ctggaaacat tgagaatctg      360

gaagaactga gaaccctgtt cagcagcagc tcttcttacc agagaatcca gctgtttcct      420

gatacaattt ggaatgtgac atacacaggc acatctaaga gctgcagcga tagcttttat      480

agaaacatga ggtggctgac acagaaaaac ggcttatacc ccatccagga cgctcagtat      540

acaaataata gaggaaagga tattctgttt gtgtggggaa tccatcaccc cccaacagat      600

actgcacaga caaatctgta caccagaact gatactacaa catctgtgac aacagaaaat      660

ctggatagaa cctttaaacc actgatcgga ccaagacctc tggtgaatgg actgattgga      720

agaattaatt actactggtc tgtgctgaaa cctggccaga cactgagagt gaggagcaac      780

ggaaacctga tcgccccctg gtttggacac attctgtctg gagagagcca cggaagaatc      840

ctgagaacag atctgagcag cggcaactgc gtggtgcagt gtcagacaga gaagggactg      900

gtgccaagag gaagcagcgg ccaccaccac caccatcacg atgagctgtg actcgaggac      960


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pEAQ-HT forward primer

<400>  11
acttgttacg attctgctga ctttcggcgg                                        30


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pHEAQ-HT reverse primer

<400>  12
cgacctgcta aacaggagct cacaaaga                                          28


<210>  13
<211>  20
<212>  DNA
<213>  FSC5 forward primer

<400>  13
ggttttcgaa cttggagaaa                                                   20


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FSC5 reverse primer

<400>  14
agaaaaccgc tcaccaaaca taga                                              24


<210>  15
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CHIR-AB1-HRP conjugate

<400>  15

Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly 
1               5                   10                  15      


Val His Cys His His His His His His Gly Ser Leu Val Pro Arg Gly 
            20                  25                  30          


Ser Gln Leu Thr Pro Thr Phe Tyr Asp Asn Ser Cys Pro Asn Val Ser 
        35                  40                  45              


Asn Ile Val Arg Asp Thr Ile Val Asn Glu Leu Arg Ser Asp Pro Arg 
    50                  55                  60                  


Ile Ala Ala Ser Ile Leu Arg Leu His Phe His Asp Cys Phe Val Asn 
65                  70                  75                  80  


Gly Cys Asp Ala Ser Ile Leu Leu Asp Asn Thr Thr Ser Phe Arg Thr 
                85                  90                  95      


Glu Lys Asp Ala Phe Gly Asn Ala Asn Ser Ala Arg Gly Phe Pro Val 
            100                 105                 110         


Ile Asp Arg Met Lys Ala Ala Val Glu Ser Ala Cys Pro Arg Thr Val 
        115                 120                 125             


Ser Cys Ala Asp Leu Leu Thr Ile Ala Ala Gln Gln Ser Val Thr Leu 
    130                 135                 140                 


Ala Gly Gly Pro Ser Trp Arg Val Pro Leu Gly Arg Arg Asp Ser Leu 
145                 150                 155                 160 


Gln Ala Phe Leu Asp Leu Ala Asn Ala Asn Leu Pro Ala Pro Phe Phe 
                165                 170                 175     


Thr Leu Pro Gln Leu Lys Asp Ser Phe Arg Asn Val Gly Leu Asn Arg 
            180                 185                 190         


Ser Ser Asp Leu Val Ala Leu Ser Gly Gly His Thr Phe Gly Lys Asn 
        195                 200                 205             


Gln Cys Arg Phe Ile Met Asp Arg Leu Tyr Asn Phe Ser Asn Thr Gly 
    210                 215                 220                 


Leu Pro Asp Pro Thr Leu Asn Thr Thr Tyr Leu Gln Thr Leu Arg Gly 
225                 230                 235                 240 


Leu Cys Pro Leu Asn Gly Asn Leu Ser Ala Leu Val Asp Phe Asp Leu 
                245                 250                 255     


Arg Thr Pro Thr Ile Phe Asp Asn Lys Tyr Tyr Val Asn Leu Glu Glu 
            260                 265                 270         


Gln Lys Gly Leu Ile Gln Ser Asp Gln Glu Leu Phe Ser Ser Pro Asn 
        275                 280                 285             


Ala Thr Asp Thr Ile Pro Leu Val Arg Ser Phe Ala Asn Ser Thr Gln 
    290                 295                 300                 


Thr Phe Phe Asn Ala Phe Val Glu Ala Met Asp Arg Met Gly Asn Ile 
305                 310                 315                 320 


Thr Pro Leu Thr Gly Thr Gln Gly Gln Ile Arg Leu Asn Cys Arg Val 
                325                 330                 335     


Val Asn Ser Asn Ser Gly Gly Gly Gly Ser Gln Gln Leu Pro Gln Pro 
            340                 345                 350         


Ser Leu Ser Leu His Pro Ser Gln Gly Val Ser Leu Gly Asp Thr Val 
        355                 360                 365             


Thr Leu Arg Cys His Leu Pro Arg Met Ala Ala Trp Val Gln Leu Trp 
    370                 375                 380                 


Leu Asn Gly Thr Leu Arg Phe Asp Lys Glu Lys Asp Lys Glu Gln Asp 
385                 390                 395                 400 


Ala Ala Glu Phe Ser Phe Ala Val Thr Asn Leu Glu Asp Ala Gly Thr 
                405                 410                 415     


Tyr Gln Cys Arg Tyr Gln Val Ser Glu Pro Leu Trp Thr Ser Asn Gln 
            420                 425                 430         


Ser Asp Pro Val Glu Leu Val Leu Thr Gly Ala Lys Glu Trp Ser His 
        435                 440                 445             


Gly Asn Leu Leu Val Ala Val Leu Arg Gly Cys Ala Ala Val Leu Val 
    450                 455                 460                 


Phe Ser Leu Gly Leu Tyr Phe Val Leu Asp Ala Arg Ser Leu Trp Thr 
465                 470                 475                 480 


Arg Arg Asp Glu Ser Ala Gly Val Ala Pro Glu Met Pro Glu Ser Val 
                485                 490                 495     


Gln Phe Gln Gly Pro Ser Arg Asp Ser Glu Asp Leu Thr Tyr Ala Glu 
            500                 505                 510         


Met Pro Ala Ala Ile Pro Cys Ser Gln Ser Pro Thr Ser Pro Thr Ala 
        515                 520                 525             


Pro Gln Ser Pro Val Ile Tyr Thr Thr Val Ser Thr Asp Leu Pro Arg 
    530                 535                 540                 


<210>  16
<211>  1653
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CHIR-AB1-HRP conjugate

<400>  16
aaaaccggta tgggatggag ctggatcttc ctgttcctgc tgagcggcgc agctggagtg       60

cactgccacc accatcacca ccatggaagc ctggtgccta gaggatctca gctgactcca      120

acattttatg acaacagctg cccaaatgtg agcaacattg tgagagatac tattgtgaac      180

gaactgagaa gtgatcctag aatagcagct agcattttga ggctgcactt tcatgattgt      240

ttcgtgaacg gatgcgacgc tagcattctg ctggataaca caacatcttt taggactgag      300

aaagatgcat ttggtaatgc taatagcgca aggggatttc ctgtgattga tagaatgaaa      360

gctgcagttg aaagcgcatg cccaagaaca gtgtcctgcg ctgatctgct gactattgca      420

gctcagcaga gcgtgaccct ggccggcggc ccaagctgga gagtgcctct gggaagaagg      480

gatagcctgc aggccttcct ggacctggcc aacgctaacc tgcccgcccc cttcttcacc      540

ctgcctcagc tgaaggactc ctttagaaac gtgggcctga acagaagcag cgacctggtg      600

gctctgagcg gcgggcacac ctttggcaag aaccagtgca gattcatcat ggataggctg      660

tacaacttca gcaacacagg cctgcccgac cccaccctga acacaaccta cctgcagaca      720

ctgagaggac tgtgccccct gaacggcaac ctgagcgccc tcgtggattt cgatctgaga      780

acccccacaa tctttgataa caagtactac gtgaacctgg aggagcagaa aggcctgatt      840

cagagcgatc aggagctgtt cagcagccca aatgccacag acaccatccc cctggtgagg      900

agcttcgcta acagcaccca gactttcttc aacgccttcg tggaggccat ggacagaatg      960

ggaaacatca cccccctgac cggcacccag ggccagatca gactgaactg ccgggtggtg     1020

aactccaaca gcggcggcgg cggcagccag cagctgccac agccaagcct gagcctgcac     1080

cctagccagg gagtgagcct gggagataca gtgactctga gatgccacct gccaagaatg     1140

gctgcttggg tgcagctgtg gctgaacggg accctgagat ttgataagga aaaggataaa     1200

gaacaggatg ccgctgagtt ttcttttgct gtgacaaatc tggaagatgc tggcacatac     1260

cagtgcagat atcaggtgtc tgagccactg tggacatcta atcagagcga tccagtggag     1320

ctggtgctga ctggagcaaa agaatggtct catggaaacc tgctggtggc agtgctgaga     1380

ggatgcgccg cagtgctggt gtttagcctg ggactgtact tcgtgctgga tgctagaagc     1440

ctgtggacca ggagagatga gtctgctggc gtggccccag agatgcctga aagcgtgcag     1500

tttcagggac ctagcagaga ctctgaagat ctgacatacg ctgagatgcc tgcagctatc     1560

ccatgttctc agtctcctac atctcctact gctccacaga gccccgtgat ctacacaact     1620

gtgagcacag atctgcctag atgactcgag aaa                                  1653


