                         SEQUENCE LISTING

<110>  NantKwest
 
<120>  ELIMINATION OF PD-L1-POSITIVE MALIGNANCIES BY PD-L1 CHIMERIC 
       ANTIGEN RECEPTOR-EXPRESSING NK CELLS

<130>  104077.0006PCT

<150>  62/753,740
<151>  2018-10-31

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  236
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A amino 
       acid sequence (mature form)

<400>  1

Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro Gln Trp 
1               5                   10                  15      


Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln Gly Ala 
            20                  25                  30          


Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu Ser Leu 
        35                  40                  45              


Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr Val Asp 
    50                  55                  60                  


Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu Ser Asp 
65                  70                  75                  80  


Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln Ala Pro 
                85                  90                  95      


Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys His Ser 
            100                 105                 110         


Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn Gly Lys 
        115                 120                 125             


Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro Lys Ala 
    130                 135                 140                 


Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Phe Gly Ser 
145                 150                 155                 160 


Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln Gly Leu 
                165                 170                 175     


Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln Val Ser 
            180                 185                 190         


Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly Leu Tyr 
        195                 200                 205             


Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp Lys Asp 
    210                 215                 220                 


His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
225                 230                 235     


<210>  2
<211>  236
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  High Affinity Variant F158V Immunoglobulin Gamma Fc Region 
       Receptor III-A amino acid sequence (mature form)

<400>  2

Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro Gln Trp 
1               5                   10                  15      


Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln Gly Ala 
            20                  25                  30          


Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu Ser Leu 
        35                  40                  45              


Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr Val Asp 
    50                  55                  60                  


Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu Ser Asp 
65                  70                  75                  80  


Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln Ala Pro 
                85                  90                  95      


Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys His Ser 
            100                 105                 110         


Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn Gly Lys 
        115                 120                 125             


Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro Lys Ala 
    130                 135                 140                 


Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val Gly Ser 
145                 150                 155                 160 


Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln Gly Leu 
                165                 170                 175     


Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln Val Ser 
            180                 185                 190         


Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly Leu Tyr 
        195                 200                 205             


Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp Lys Asp 
    210                 215                 220                 


His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
225                 230                 235     


<210>  3
<211>  254
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A amino 
       acid sequence (precursor form)

<400>  3

Met Trp Gln Leu Leu Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala 
1               5                   10                  15      


Gly Met Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro 
            20                  25                  30          


Gln Trp Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln 
        35                  40                  45              


Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu 
    50                  55                  60                  


Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr 
65                  70                  75                  80  


Val Asp Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu 
                85                  90                  95      


Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln 
            100                 105                 110         


Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys 
        115                 120                 125             


His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn 
    130                 135                 140                 


Gly Lys Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro 
145                 150                 155                 160 


Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Phe 
                165                 170                 175     


Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln 
            180                 185                 190         


Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln 
        195                 200                 205             


Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly 
    210                 215                 220                 


Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp 
225                 230                 235                 240 


Lys Asp His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
                245                 250                 


<210>  4
<211>  254
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  High Affinity Variant Immunoglobulin Gamma Fc Region Receptor 
       III-A amino acid sequence (precursor form)

<400>  4

Met Trp Gln Leu Leu Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala 
1               5                   10                  15      


Gly Met Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro 
            20                  25                  30          


Gln Trp Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln 
        35                  40                  45              


Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu 
    50                  55                  60                  


Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr 
65                  70                  75                  80  


Val Asp Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu 
                85                  90                  95      


Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln 
            100                 105                 110         


Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys 
        115                 120                 125             


His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn 
    130                 135                 140                 


Gly Lys Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro 
145                 150                 155                 160 


Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val 
                165                 170                 175     


Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln 
            180                 185                 190         


Gly Leu Ala Val Ser Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln 
        195                 200                 205             


Val Ser Phe Cys Leu Val Met Val Leu Leu Phe Ala Val Asp Thr Gly 
    210                 215                 220                 


Leu Tyr Phe Ser Val Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp 
225                 230                 235                 240 


Lys Asp His Lys Phe Lys Trp Arg Lys Asp Pro Gln Asp Lys 
                245                 250                 


<210>  5
<211>  765
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Polynucleotide Encoding the Low Affinity Immunoglobulin Gamma Fc 
       Region Receptor III-A (Precursor)

<400>  5
atgtggcagc tgctcctccc aactgctctg ctacttctag tttcagctgg catgcggact       60

gaagatctcc caaaggctgt ggtgttcctg gagcctcaat ggtacagggt gctcgagaag      120

gacagtgtga ctctgaagtg ccagggagcc tactcccctg aggacaattc cacacagtgg      180

tttcacaatg agagcctcat ctcaagccag gcctcgagct acttcattga cgctgccaca      240

gtcgacgaca gtggagagta caggtgccag acaaacctct ccaccctcag tgacccggtg      300

cagctagaag tccatatcgg ctggctgttg ctccaggccc ctcggtgggt gttcaaggag      360

gaagacccta ttcacctgag gtgtcacagc tggaagaaca ctgctctgca taaggtcaca      420

tatttacaga atggcaaagg caggaagtat tttcatcata attctgactt ctacattcca      480

aaagccacac tcaaagacag cggctcctac ttctgcaggg ggctttttgg gagtaaaaat      540

gtgtcttcag agactgtgaa catcaccatc actcaaggtt tggcagtgtc aaccatctca      600

tcattctttc cacctgggta ccaagtctct ttctgcttgg tgatggtact cctttttgca      660

gtggacacag gactatattt ctctgtgaag acaaacattc gaagctcaac aagagactgg      720

aaggaccata aatttaaatg gagaaaggac cctcaagaca aatga                      765


<210>  6
<211>  153
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Wild-Type IL-2

<400>  6

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
145                 150             


<210>  7
<211>  160
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  er-IL2

<400>  7

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Ser Glu Lys Asp Glu Leu 
145                 150                 155                 160 


<210>  8
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial P2A sequence


<220>
<221>  misc_feature
<223>  P2A sequence

<400>  8
ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct       60

ggacct                                                                  66


<210>  9
<211>  807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial codon optimized PD-L1 scFv sequence

<400>  9
atggactgga tctggcggat tctgtttctc gtgggagctg ccacaggcgc tcattctgct       60

cagcctgcca acatccagat gacccagtct ccatcttctg tgtctgcatc tgtaggagac      120

agagtcacca tcacttgtcg ggcgagtcag gatattagcc gctggttagc ctggtatcag      180

cagaaaccag ggaaagcccc taaactcctg atctatgctg catccagttt gcaaagtggg      240

gtcccatcga ggttcagcgg cagtggatct gggacagatt tcgctctcac tatcagcagc      300

ctgcagcctg aagattttgc aacttactat tgtcaacagg ctgacagtcg tttctcgatc      360

accttcggcc aagggacacg actggagatt aaaggcggcg gaggaagcgg aggcggagga      420

tctgggggcg gaggctctgg cggaggggga tctgaggtgc agctggtgca gtctggggga      480

ggcttggtac agcctggggg gtccctgaga ctctcctgtg cagcctctgg attcaccttc      540

agtagctata gcatgaactg ggtccgccag gctccaggga aggggctgga gtgggtttca      600

tacattagta gtagtagtag taccatacag tacgcagact ctgtgaaggg ccgattcacc      660

atctccagag acaatgccaa gaactcactg tatctgcaaa tgaacagcct gagagacgag      720

gacacggctg tgtattactg tgcgagaggg gactactact acggtatgga cgtctggggc      780

caagggacca cggtcaccgt gagctca                                          807


<210>  10
<211>  269
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial PD-L1 scFv sequence

<400>  10

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Ala Gln Pro Ala Asn Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Ile Ser Arg Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ala Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ala Asp Ser Arg Phe Ser Ile Thr Phe Gly Gln Gly Thr Arg Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Gln Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Phe Ser Ser Tyr Ser Met Asn Trp Val Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Ser Ser Ser Thr 
        195                 200                 205             


Ile Gln Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Asp Tyr Tyr Tyr Gly Met 
                245                 250                 255     


Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
            260                 265                 


<210>  11
<211>  6772
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial tricistronic construct encoding PD-L1 CAR, CD16, and 
       erIL-2


<220>
<221>  anti PD-L1 scFv sequence
<222>  (1552)..(2358)

<220>
<221>  CD8 hinge
<222>  (2367)..(2559)

<220>
<221>  CD28 transmembrane
<222>  (2560)..(2635)

<220>
<221>  FceRIg signaling domain
<222>  (2636)..(2766)

<220>
<221>  P2A sequence
<222>  (2767)..(2832)

<220>
<221>  CD16 sequence
<222>  (2833)..(3597)

<220>
<221>  ERIL-2 sequence
<222>  (4199)..(4681)

<400>  11
tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct       60

gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct      120

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt      180

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga      240

atctgcttag ggttaggcgt tttgcgctgc ttcgggatcc gctgaccaaa agagcaccaa      300

aggcgccctg accttcagcc cctacctgcg ctccggtgcc cgtcagtggg cagagcgcac      360

atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag      420

aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga      480

gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg      540

gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac      600

gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga      660

tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc      720

ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg      780

gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg      840

atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct      900

gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg      960

cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc     1020

tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg     1080

ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg     1140

ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc     1200

acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga     1260

gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt     1320

aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga     1380

agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg     1440

atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt     1500

gtcgtgataa tacgactcac tatagggaga cccaagctgg aattcgccac catggactgg     1560

atctggcgga ttctgtttct cgtgggagct gccacaggcg ctcattctgc tcagcctgcc     1620

aacatccaga tgacccagtc tccatcttct gtgtctgcat ctgtaggaga cagagtcacc     1680

atcacttgtc gggcgagtca ggatattagc cgctggttag cctggtatca gcagaaacca     1740

gggaaagccc ctaaactcct gatctatgct gcatccagtt tgcaaagtgg ggtcccatcg     1800

aggttcagcg gcagtggatc tgggacagat ttcgctctca ctatcagcag cctgcagcct     1860

gaagattttg caacttacta ttgtcaacag gctgacagtc gtttctcgat caccttcggc     1920

caagggacac gactggagat taaaggcggc ggaggaagcg gaggcggagg atctgggggc     1980

ggaggctctg gcggaggggg atctgaggtg cagctggtgc agtctggggg aggcttggta     2040

cagcctgggg ggtccctgag actctcctgt gcagcctctg gattcacctt cagtagctat     2100

agcatgaact gggtccgcca ggctccaggg aaggggctgg agtgggtttc atacattagt     2160

agtagtagta gtaccataca gtacgcagac tctgtgaagg gccgattcac catctccaga     2220

gacaatgcca agaactcact gtatctgcaa atgaacagcc tgagagacga ggacacggct     2280

gtgtattact gtgcgagagg ggactactac tacggtatgg acgtctgggg ccaagggacc     2340

acggtcaccg tgagctcagc ggccgcgctg agcaacagca tcatgtactt cagccacttc     2400

gtgcctgtgt tcctgcctgc caagcctaca acaacaccag cccctagacc tccaacccct     2460

gcccctacaa ttgcctctca gcctctgtct ctgaggcccg aagcttgtag acctgctgct     2520

ggcggagctg tgcacaccag aggactggat ttcgcctgct tttgggtgct ggtggtcgtg     2580

ggcggagtgc tggcttgtta ttctctgctg gtcaccgtgg ccttcatcat cttttgggtc     2640

cgactgaaga tccaggtccg aaaggccgcc atcaccagct acgagaagtc tgatggcgtg     2700

tacaccggcc tgagcaccag aaaccaggaa acctacgaga cactgaagca cgagaagccc     2760

ccccagggat ctggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag     2820

aaccctggac ctatgtggca gctgctgctg cctacagctc tcctgctgct ggtgtccgcc     2880

ggcatgagaa ccgaggatct gcctaaggcc gtggtgttcc tggaacccca gtggtacaga     2940

gtgctggaaa aggacagcgt gaccctgaag tgccagggcg cctacagccc cgaggacaat     3000

agcacccagt ggttccacaa cgagagcctg atcagcagcc aggccagcag ctacttcatc     3060

gacgccgcca ccgtggacga cagcggcgag tatagatgcc agaccaacct gagcaccctg     3120

agcgaccccg tgcagctgga agtgcacatc ggatggctgc tgctgcaggc ccccagatgg     3180

gtgttcaaag aagaggaccc catccacctg agatgccact cttggaagaa caccgccctg     3240

cacaaagtga cctacctgca gaacggcaag ggcagaaagt acttccacca caacagcgac     3300

ttctacatcc ccaaggccac cctgaaggac tccggctcct acttctgcag aggcctcgtg     3360

ggcagcaaga acgtgtccag cgagacagtg aacatcacca tcacccaggg cctggccgtg     3420

tctaccatca gcagcttttt cccacccggc taccaggtgt ccttctgcct cgtgatggtg     3480

ctgctgttcg ccgtggacac cggcctgtac ttcagcgtga aaacaaacat cagaagcagc     3540

acccgggact ggaaggacca caagttcaag tggcggaagg acccccagga caagtgaaat     3600

tccgcccctc tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc     3660

gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt     3720

ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc     3780

tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc     3840

tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc     3900

cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg     3960

cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct     4020

cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat     4080

ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc     4140

taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa ccgccaccat     4200

gtaccggatg cagctgctga gctgtatcgc cctgtctctg gccctcgtga ccaacagcgc     4260

ccctaccagc agcagcacca agaaaaccca gctgcagctg gaacatctgc tgctggacct     4320

gcagatgatc ctgaacggca tcaacaacta caagaacccc aagctgaccc ggatgctgac     4380

cttcaagttc tacatgccca agaaggccac cgaactgaaa catctgcagt gcctggaaga     4440

ggaactgaag cccctggaag aagtgctgaa cctggcccag agcaagaact tccacctgag     4500

gcccagggac ctgatcagca acatcaacgt gatcgtgctg gaactgaaag gcagcgagac     4560

aaccttcatg tgcgagtacg ccgacgagac agctaccatc gtggaatttc tgaaccggtg     4620

gatcaccttc tgccagagca tcatcagcac cctgaccggc tccgagaagg acgagctgtg     4680

agcggccgcc cgctgatcag cctcgaacga gatttcgatt ccaccgccgc cttctatgaa     4740

aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat     4800

ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa     4860

taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt     4920

ggtttgtcca aactcatcaa tgtatcttat catgtctgtg cggtgggctc tatggcttct     4980

gaggcggaaa gaaccagctg gggctctagg gggtatcccc ggatcctgag caaaaggcca     5040

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5100

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     5160

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     5220

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5280

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5340

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5400

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5460

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     5520

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     5580

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     5640

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     5700

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     5760

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     5820

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     5880

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     5940

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc     6000

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     6060

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     6120

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     6180

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     6240

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     6300

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     6360

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     6420

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     6480

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     6540

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     6600

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     6660

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     6720

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aa             6772


<210>  12
<211>  681
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence for PD-L1 CAR and CD16 fusion protein


<220>
<221>  CD8 hinge
<222>  (273)..(336)

<220>
<221>  CD28 transmembrane
<222>  (337)..(364)

<220>
<221>  FceRIg signaling domain
<222>  (365)..(405)

<220>
<221>  P2A sequence
<222>  (406)..(427)

<220>
<221>  CD16 sequence
<222>  (428)..(681)

<400>  12

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Ala Gln Pro Ala Asn Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Ile Ser Arg Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ala Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ala Asp Ser Arg Phe Ser Ile Thr Phe Gly Gln Gly Thr Arg Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Gln Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Phe Ser Ser Tyr Ser Met Asn Trp Val Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Ser Ser Ser Thr 
        195                 200                 205             


Ile Gln Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Asp Tyr Tyr Tyr Gly Met 
                245                 250                 255     


Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ala Ala 
            260                 265                 270         


Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe Leu 
        275                 280                 285             


Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
    290                 295                 300                 


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
305                 310                 315                 320 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
                325                 330                 335     


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            340                 345                 350         


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Leu Lys Ile Gln 
        355                 360                 365             


Val Arg Lys Ala Ala Ile Thr Ser Tyr Glu Lys Ser Asp Gly Val Tyr 
    370                 375                 380                 


Thr Gly Leu Ser Thr Arg Asn Gln Glu Thr Tyr Glu Thr Leu Lys His 
385                 390                 395                 400 


Glu Lys Pro Pro Gln Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys 
                405                 410                 415     


Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Met Trp Gln Leu Leu 
            420                 425                 430         


Leu Pro Thr Ala Leu Leu Leu Leu Val Ser Ala Gly Met Arg Thr Glu 
        435                 440                 445             


Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro Gln Trp Tyr Arg Val 
    450                 455                 460                 


Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln Gly Ala Tyr Ser Pro 
465                 470                 475                 480 


Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu Ser Leu Ile Ser Ser 
                485                 490                 495     


Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr Val Asp Asp Ser Gly 
            500                 505                 510         


Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu Ser Asp Pro Val Gln 
        515                 520                 525             


Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln Ala Pro Arg Trp Val 
    530                 535                 540                 


Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys His Ser Trp Lys Asn 
545                 550                 555                 560 


Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn Gly Lys Gly Arg Lys 
                565                 570                 575     


Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro Lys Ala Thr Leu Lys 
            580                 585                 590         


Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val Gly Ser Lys Asn Val 
        595                 600                 605             


Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln Gly Leu Ala Val Ser 
    610                 615                 620                 


Thr Ile Ser Ser Phe Phe Pro Pro Gly Tyr Gln Val Ser Phe Cys Leu 
625                 630                 635                 640 


Val Met Val Leu Leu Phe Ala Val Asp Thr Gly Leu Tyr Phe Ser Val 
                645                 650                 655     


Lys Thr Asn Ile Arg Ser Ser Thr Arg Asp Trp Lys Asp His Lys Phe 
            660                 665                 670         


Lys Trp Arg Lys Asp Pro Gln Asp Lys 
        675                 680     


<210>  13
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence of erIL2

<400>  13

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Ser Glu Lys Asp Glu Leu 
145                 150                 155                 160 


<210>  14
<211>  1215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial sequence encoding PD-L1 CAR

<400>  14
atggactgga tctggcggat tctgtttctc gtgggagctg ccacaggcgc tcattctgct       60

cagcctgcca acatccagat gacccagtct ccatcttctg tgtctgcatc tgtaggagac      120

agagtcacca tcacttgtcg ggcgagtcag gatattagcc gctggttagc ctggtatcag      180

cagaaaccag ggaaagcccc taaactcctg atctatgctg catccagttt gcaaagtggg      240

gtcccatcga ggttcagcgg cagtggatct gggacagatt tcgctctcac tatcagcagc      300

ctgcagcctg aagattttgc aacttactat tgtcaacagg ctgacagtcg tttctcgatc      360

accttcggcc aagggacacg actggagatt aaaggcggcg gaggaagcgg aggcggagga      420

tctgggggcg gaggctctgg cggaggggga tctgaggtgc agctggtgca gtctggggga      480

ggcttggtac agcctggggg gtccctgaga ctctcctgtg cagcctctgg attcaccttc      540

agtagctata gcatgaactg ggtccgccag gctccaggga aggggctgga gtgggtttca      600

tacattagta gtagtagtag taccatacag tacgcagact ctgtgaaggg ccgattcacc      660

atctccagag acaatgccaa gaactcactg tatctgcaaa tgaacagcct gagagacgag      720

gacacggctg tgtattactg tgcgagaggg gactactact acggtatgga cgtctggggc      780

caagggacca cggtcaccgt gagctcagcg gccgcgctga gcaacagcat catgtacttc      840

agccacttcg tgcctgtgtt cctgcctgcc aagcctacaa caacaccagc ccctagacct      900

ccaacccctg cccctacaat tgcctctcag cctctgtctc tgaggcccga agcttgtaga      960

cctgctgctg gcggagctgt gcacaccaga ggactggatt tcgcctgctt ttgggtgctg     1020

gtggtcgtgg gcggagtgct ggcttgttat tctctgctgg tcaccgtggc cttcatcatc     1080

ttttgggtcc gactgaagat ccaggtccga aaggccgcca tcaccagcta cgagaagtct     1140

gatggcgtgt acaccggcct gagcaccaga aaccaggaaa cctacgagac actgaagcac     1200

gagaagcccc cccag                                                      1215


<210>  15
<211>  405
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequenc for PD-L1 CAR

<400>  15

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Ala Gln Pro Ala Asn Ile Gln Met Thr Gln Ser Pro Ser 
            20                  25                  30          


Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala 
        35                  40                  45              


Ser Gln Asp Ile Ser Arg Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly 
    50                  55                  60                  


Lys Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Ser Leu Gln Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ala Leu 
                85                  90                  95      


Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Gln Ala Asp Ser Arg Phe Ser Ile Thr Phe Gly Gln Gly Thr Arg Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Gln Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Phe Ser Ser Tyr Ser Met Asn Trp Val Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Tyr Ile Ser Ser Ser Ser Ser Thr 
        195                 200                 205             


Ile Gln Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Asp Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly Asp Tyr Tyr Tyr Gly Met 
                245                 250                 255     


Asp Val Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ala Ala 
            260                 265                 270         


Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe Val Pro Val Phe Leu 
        275                 280                 285             


Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
    290                 295                 300                 


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
305                 310                 315                 320 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
                325                 330                 335     


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
            340                 345                 350         


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Leu Lys Ile Gln 
        355                 360                 365             


Val Arg Lys Ala Ala Ile Thr Ser Tyr Glu Lys Ser Asp Gly Val Tyr 
    370                 375                 380                 


Thr Gly Leu Ser Thr Arg Asn Gln Glu Thr Tyr Glu Thr Leu Lys His 
385                 390                 395                 400 


Glu Lys Pro Pro Gln 
                405 


