                         SEQUENCE LISTING

<110>  Syngenta Participations AG
       REYNOLDS, Clarence Michael
 
<120>  Insecticidal Proteins

<130>  81599-WO-REG-ORG-P-1

<150>  62/752,546
<151>  2018-10-30

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  489
<212>  PRT
<213>  Serratia proteamaculans

<400>  1

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  2
<211>  488
<212>  PRT
<213>  Serratia plymuthica

<400>  2

Met Lys Phe Glu Ser Leu Lys Val Glu Asn Leu Phe Ser Ser Ser Leu 
1               5                   10                  15      


Thr Arg Ile Asn Ala Ile Pro Leu Pro Asn Asp Ala Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ser 
        35                  40                  45              


Ser Ala Val Leu His Pro Val Leu Asp Trp Ser Lys Ser Gln Phe His 
    50                  55                  60                  


Thr Val Thr Met Asn Gly Gln Thr Tyr Gln Leu Pro Glu Ile Leu Asn 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Thr Tyr Ser Ser Val Ser Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ser Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Thr Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ala Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Ile Ser Asp Asp Phe Lys Lys Leu Ile Ser Ser Ile Asp Pro Asn 
                165                 170                 175     


Asn Gln Gln Thr Leu Ala Thr Ile Phe Asn Val Tyr Gly Ser His Ile 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Ala Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Glu Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Val Asn Asn Gln Asp Ser Asp Asp Asn Leu Arg Lys Trp Ile Asp Ser 
        275                 280                 285             


Ile Ser Thr Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser Leu 
    290                 295                 300                 


Leu Pro Val Trp Thr Leu Cys Glu Asp Gln Val Lys Ala Asp Ala Leu 
305                 310                 315                 320 


Lys Asn Tyr Tyr Asn Asn Thr Trp Ser Gln Leu Gln Met Ala Val Ala 
                325                 330                 335     


Arg Leu Arg Ala Asn Tyr Ile Asp Glu Val Thr Phe Val Leu Gly Asp 
            340                 345                 350         


Asn Ser Asp Ile Pro Ala Pro Ala Gly Tyr Thr Lys Val Pro Val Asp 
        355                 360                 365             


Leu Asn Ser Gly Ala Gly Gly Lys Phe Ile Tyr Leu Cys Tyr His Glu 
    370                 375                 380                 


Ala Gln Phe Thr Pro Val Asn Ser Lys Gln Ala Ile Val Gly Leu Gln 
385                 390                 395                 400 


Val Leu Tyr Gly Lys Gln Glu Pro Ala Pro Asp Tyr Ser Arg Ile Asn 
                405                 410                 415     


Ile Asp Leu Asn Ser Gly Ala Asn Gly Asp Asp Val Tyr Leu Ser Tyr 
            420                 425                 430         


Lys Lys Gly Asp Ala Thr Ser Lys Glu Val Ile Asn Lys Ile Thr Ala 
        435                 440                 445             


Val Tyr Gly Lys Asp Gln Tyr Val Pro Thr Pro Tyr Gly Tyr Lys Gln 
    450                 455                 460                 


Ile Pro Gly Asp Leu Asn Ser Gly Ala Gly Gly Asp Phe Val Tyr Phe 
465                 470                 475                 480 


Cys Thr Tyr Gln Gly Gly Thr Glu 
                485             


<210>  3
<211>  489
<212>  PRT
<213>  Serratia quinivorans

<400>  3

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe His 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Leu Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Ala Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Ser Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  4
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW V8A L11I

<400>  4

Met Lys Ile Glu Ser Ser Lys Ala Glu Gly Ile Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  5
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW Y188W


<220>
<221>  MISC_FEATURE
<222>  (188)..(188)
<223>  Xaa = W or F

<400>  5

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Xaa Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  6
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW Y413W

<400>  6

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Trp Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  7
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW F428W

<400>  7

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Trp Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  8
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW Y430W

<400>  8

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Trp Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  9
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW S190P

<400>  9

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Pro His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  10
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW V192Y

<400>  10

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Tyr 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  11
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW C312L

<400>  11

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Leu Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  12
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW C382A


<220>
<221>  MISC_FEATURE
<222>  (382)..(382)
<223>  Xaa = A, S, T, M, G, F or L.

<400>  12

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Xaa Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  13
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW C482L

<400>  13

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Leu Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  14
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW V398L

<400>  14

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Leu Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  15
<211>  491
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW P396-L-L-I397

<400>  15

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Leu Leu Ile Val 
385                 390                 395                 400 


Asp Ile Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile 
                405                 410                 415     


Lys Ile Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr 
            420                 425                 430         


Leu Ser Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys 
        435                 440                 445             


Ile Thr Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly 
    450                 455                 460                 


Tyr Lys Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe 
465                 470                 475                 480 


Val Tyr Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 490     


<210>  16
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW I397L

<400>  16

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Leu Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  17
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW I397L V398L

<400>  17

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Leu Leu Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Cys Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  18
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW Y480L C482L

<400>  18

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Leu 
465                 470                 475                 480 


Leu Leu Thr Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  19
<211>  489
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SproCRW C482L T483L

<400>  19

Met Lys Ile Glu Ser Ser Lys Val Glu Gly Leu Phe Ser Ser Ser Phe 
1               5                   10                  15      


Thr Arg Val Asn Ala Val Pro Leu Pro Ser Asp Thr Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ala 
        35                  40                  45              


Ser Ala Val Leu His Pro Ile Leu Asp Trp Ser Lys Ser Gln Phe Asn 
    50                  55                  60                  


Glu Ile Thr Met Asn Gly Gln Gln Tyr Gln Leu Pro Asp Val Leu Gln 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Ser Tyr Ala Ser Val Thr Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ala Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Ser Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ile Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Val Ser Asp Asp Phe Ile Lys Leu Ile Asn Ala Ile Asn Pro Glu 
                165                 170                 175     


Ser Lys Asp Ser Ile Ala Thr Val Phe Asn Val Tyr Gly Ser His Val 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Val Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Asp Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Asn Asn Ser Gln Gly Ser Ser Asp Asp Asn Leu Lys Asn Trp Ile Gln 
        275                 280                 285             


Ser Ile Ser Ser Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser 
    290                 295                 300                 


Leu Met Pro Val Trp Leu Leu Cys Glu Asp Lys Thr Lys Ala Asp Ala 
305                 310                 315                 320 


Leu Lys Lys Tyr Tyr Asp Asn Thr Trp Ser Lys Ser Gln Met Ala Val 
                325                 330                 335     


Ala Ser Leu Arg Ala Asn Tyr Ile Asp Glu Leu Thr Phe Val Leu Gly 
            340                 345                 350         


Asp Asn Ser Asp Ile Pro Ala Pro Val Gly Tyr Thr Lys Val Pro Ile 
        355                 360                 365             


Asp Leu Asn Ser Asp Ala Gly Gly Lys Tyr Val Tyr Leu Cys Tyr His 
    370                 375                 380                 


Glu Ala Gln Phe Thr Pro Val Asn Gly Lys Gln Pro Ile Val Asp Ile 
385                 390                 395                 400 


Gln Val Leu Tyr Gly Ser Gln Met Pro Ala Pro Gly Tyr Ile Lys Ile 
                405                 410                 415     


Asp Val Asp Leu Asn Ser Gly Ala Gly Gly Glu Phe Val Tyr Leu Ser 
            420                 425                 430         


Tyr Lys Lys Gly Glu Pro Thr Ser Ser Asp Val Ile Asn Lys Ile Thr 
        435                 440                 445             


Ala Val Tyr Gly Lys Asn Glu Tyr Val Pro Thr Pro Tyr Gly Tyr Lys 
    450                 455                 460                 


Gln Ile Ser Gly Asp Leu Asn Ala Gly Ala Gly Gly Asp Phe Val Tyr 
465                 470                 475                 480 


Leu Leu Leu Tyr Gln Gly Gly Thr Glu 
                485                 


<210>  20
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SplyCRW C481L

<400>  20

Met Lys Phe Glu Ser Leu Lys Val Glu Asn Leu Phe Ser Ser Ser Leu 
1               5                   10                  15      


Thr Arg Ile Asn Ala Ile Pro Leu Pro Asn Asp Ala Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ser 
        35                  40                  45              


Ser Ala Val Leu His Pro Val Leu Asp Trp Ser Lys Ser Gln Phe His 
    50                  55                  60                  


Thr Val Thr Met Asn Gly Gln Thr Tyr Gln Leu Pro Glu Ile Leu Asn 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Thr Tyr Ser Ser Val Ser Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ser Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Thr Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ala Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Ile Ser Asp Asp Phe Lys Lys Leu Ile Ser Ser Ile Asp Pro Asn 
                165                 170                 175     


Asn Gln Gln Thr Leu Ala Thr Ile Phe Asn Val Tyr Gly Ser His Ile 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Ala Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Glu Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Val Asn Asn Gln Asp Ser Asp Asp Asn Leu Arg Lys Trp Ile Asp Ser 
        275                 280                 285             


Ile Ser Thr Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser Leu 
    290                 295                 300                 


Leu Pro Val Trp Thr Leu Cys Glu Asp Gln Val Lys Ala Asp Ala Leu 
305                 310                 315                 320 


Lys Asn Tyr Tyr Asn Asn Thr Trp Ser Gln Leu Gln Met Ala Val Ala 
                325                 330                 335     


Arg Leu Arg Ala Asn Tyr Ile Asp Glu Val Thr Phe Val Leu Gly Asp 
            340                 345                 350         


Asn Ser Asp Ile Pro Ala Pro Ala Gly Tyr Thr Lys Val Pro Val Asp 
        355                 360                 365             


Leu Asn Ser Gly Ala Gly Gly Lys Phe Ile Tyr Leu Cys Tyr His Glu 
    370                 375                 380                 


Ala Gln Phe Thr Pro Val Asn Ser Lys Gln Ala Ile Val Gly Leu Gln 
385                 390                 395                 400 


Val Leu Tyr Gly Lys Gln Glu Pro Ala Pro Asp Tyr Ser Arg Ile Asn 
                405                 410                 415     


Ile Asp Leu Asn Ser Gly Ala Asn Gly Asp Asp Val Tyr Leu Ser Tyr 
            420                 425                 430         


Lys Lys Gly Asp Ala Thr Ser Lys Glu Val Ile Asn Lys Ile Thr Ala 
        435                 440                 445             


Val Tyr Gly Lys Asp Gln Tyr Val Pro Thr Pro Tyr Gly Tyr Lys Gln 
    450                 455                 460                 


Ile Pro Gly Asp Leu Asn Ser Gly Ala Gly Gly Asp Phe Val Tyr Phe 
465                 470                 475                 480 


Leu Thr Tyr Gln Gly Gly Thr Glu 
                485             


<210>  21
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sply Y479L C481L

<400>  21

Met Lys Phe Glu Ser Leu Lys Val Glu Asn Leu Phe Ser Ser Ser Leu 
1               5                   10                  15      


Thr Arg Ile Asn Ala Ile Pro Leu Pro Asn Asp Ala Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ser 
        35                  40                  45              


Ser Ala Val Leu His Pro Val Leu Asp Trp Ser Lys Ser Gln Phe His 
    50                  55                  60                  


Thr Val Thr Met Asn Gly Gln Thr Tyr Gln Leu Pro Glu Ile Leu Asn 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Thr Tyr Ser Ser Val Ser Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ser Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Thr Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ala Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Ile Ser Asp Asp Phe Lys Lys Leu Ile Ser Ser Ile Asp Pro Asn 
                165                 170                 175     


Asn Gln Gln Thr Leu Ala Thr Ile Phe Asn Val Tyr Gly Ser His Ile 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Ala Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Glu Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Val Asn Asn Gln Asp Ser Asp Asp Asn Leu Arg Lys Trp Ile Asp Ser 
        275                 280                 285             


Ile Ser Thr Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser Leu 
    290                 295                 300                 


Leu Pro Val Trp Thr Leu Cys Glu Asp Gln Val Lys Ala Asp Ala Leu 
305                 310                 315                 320 


Lys Asn Tyr Tyr Asn Asn Thr Trp Ser Gln Leu Gln Met Ala Val Ala 
                325                 330                 335     


Arg Leu Arg Ala Asn Tyr Ile Asp Glu Val Thr Phe Val Leu Gly Asp 
            340                 345                 350         


Asn Ser Asp Ile Pro Ala Pro Ala Gly Tyr Thr Lys Val Pro Val Asp 
        355                 360                 365             


Leu Asn Ser Gly Ala Gly Gly Lys Phe Ile Tyr Leu Cys Tyr His Glu 
    370                 375                 380                 


Ala Gln Phe Thr Pro Val Asn Ser Lys Gln Ala Ile Val Gly Leu Gln 
385                 390                 395                 400 


Val Leu Tyr Gly Lys Gln Glu Pro Ala Pro Asp Tyr Ser Arg Ile Asn 
                405                 410                 415     


Ile Asp Leu Asn Ser Gly Ala Asn Gly Asp Asp Val Tyr Leu Ser Tyr 
            420                 425                 430         


Lys Lys Gly Asp Ala Thr Ser Lys Glu Val Ile Asn Lys Ile Thr Ala 
        435                 440                 445             


Val Tyr Gly Lys Asp Gln Tyr Val Pro Thr Pro Tyr Gly Tyr Lys Gln 
    450                 455                 460                 


Ile Pro Gly Asp Leu Asn Ser Gly Ala Gly Gly Asp Phe Val Leu Phe 
465                 470                 475                 480 


Leu Thr Tyr Gln Gly Gly Thr Glu 
                485             


<210>  22
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sply C481L T482L

<400>  22

Met Lys Phe Glu Ser Leu Lys Val Glu Asn Leu Phe Ser Ser Ser Leu 
1               5                   10                  15      


Thr Arg Ile Asn Ala Ile Pro Leu Pro Asn Asp Ala Leu Pro Gly Val 
            20                  25                  30          


Gly Ile Ile Gly Cys Gly Tyr Asn Pro Phe Leu Ala Tyr Ala Asp Ser 
        35                  40                  45              


Ser Ala Val Leu His Pro Val Leu Asp Trp Ser Lys Ser Gln Phe His 
    50                  55                  60                  


Thr Val Thr Met Asn Gly Gln Thr Tyr Gln Leu Pro Glu Ile Leu Asn 
65                  70                  75                  80  


Ala Val Trp Leu Ser Asn Gln Thr Tyr Ser Ser Val Ser Gly Lys Ser 
                85                  90                  95      


Leu Gln Ser Tyr Leu Thr Glu Leu Ser Asn Ser Ile Lys Val Ser Gly 
            100                 105                 110         


Asn Tyr Gly Phe Phe Ser Ala Ser Ala Thr Asn Glu Phe Thr Asp Ser 
        115                 120                 125             


Ser Leu Arg Lys Ser Glu Asn Glu Phe Ser Arg Cys Gln Gln Ser Phe 
    130                 135                 140                 


Asp Leu Trp Ser Ile Ser Ala Pro Ala Asp Ile Ala Arg Leu Gln Asn 
145                 150                 155                 160 


Tyr Ile Ser Asp Asp Phe Lys Lys Leu Ile Ser Ser Ile Asp Pro Asn 
                165                 170                 175     


Asn Gln Gln Thr Leu Ala Thr Ile Phe Asn Val Tyr Gly Ser His Ile 
            180                 185                 190         


Leu Met Ser Gly Val Met Gly Gly Lys Ala His Val Ser Ala Ser Ala 
        195                 200                 205             


Asn Lys Leu Thr Leu Thr Gln Lys Phe Glu Met Ser Thr Ile Val Gln 
    210                 215                 220                 


Ala Lys Tyr Glu Gln Leu Thr Ser Gln Leu Ser Ala Glu Asp Lys Leu 
225                 230                 235                 240 


Lys Tyr Ser Glu Ala Phe Glu Ser Phe Ser Glu Ser Gly Ser Tyr Thr 
                245                 250                 255     


Tyr Asp Ile Leu Gly Gly Ser Pro Ser Leu Gly Ala Leu Val Phe Lys 
            260                 265                 270         


Val Asn Asn Gln Asp Ser Asp Asp Asn Leu Arg Lys Trp Ile Asp Ser 
        275                 280                 285             


Ile Ser Thr Met Pro Val Leu Thr Lys Phe Ile Asp Gln Thr Ser Leu 
    290                 295                 300                 


Leu Pro Val Trp Thr Leu Cys Glu Asp Gln Val Lys Ala Asp Ala Leu 
305                 310                 315                 320 


Lys Asn Tyr Tyr Asn Asn Thr Trp Ser Gln Leu Gln Met Ala Val Ala 
                325                 330                 335     


Arg Leu Arg Ala Asn Tyr Ile Asp Glu Val Thr Phe Val Leu Gly Asp 
            340                 345                 350         


Asn Ser Asp Ile Pro Ala Pro Ala Gly Tyr Thr Lys Val Pro Val Asp 
        355                 360                 365             


Leu Asn Ser Gly Ala Gly Gly Lys Phe Ile Tyr Leu Cys Tyr His Glu 
    370                 375                 380                 


Ala Gln Phe Thr Pro Val Asn Ser Lys Gln Ala Ile Val Gly Leu Gln 
385                 390                 395                 400 


Val Leu Tyr Gly Lys Gln Glu Pro Ala Pro Asp Tyr Ser Arg Ile Asn 
                405                 410                 415     


Ile Asp Leu Asn Ser Gly Ala Asn Gly Asp Asp Val Tyr Leu Ser Tyr 
            420                 425                 430         


Lys Lys Gly Asp Ala Thr Ser Lys Glu Val Ile Asn Lys Ile Thr Ala 
        435                 440                 445             


Val Tyr Gly Lys Asp Gln Tyr Val Pro Thr Pro Tyr Gly Tyr Lys Gln 
    450                 455                 460                 


Ile Pro Gly Asp Leu Asn Ser Gly Ala Gly Gly Asp Phe Val Tyr Phe 
465                 470                 475                 480 


Leu Leu Tyr Gln Gly Gly Thr Glu 
                485             


<210>  23
<211>  1470
<212>  DNA
<213>  Serratia proteamaculans

<400>  23
atgaaaattg aatcatccaa ggttgaaggt ttgtttagct catcttttac tcgtgttaat       60

gctgtccctc tcccgagcga tactttaccc ggcgtaggta ttattggttg tggctacaat      120

ccatttttag cctatgcaga tgcttctgct gtcctgcatc ctattctcga ctggtcaaaa      180

tctcagttta acgaaattac tatgaacggt cagcaatatc aattacctga tgtgcttcag      240

gcggtatggc tcagtaacca gtcttacgca tcggtaacgg ggaaatcgct ccaaagttat      300

ttaactgaat tggctaacag tattaaagta agtggtaact atggcttttt tagtgcctca      360

gccacaaatg aatttagcga cagttcattg agaaagtcag aaaatgagtt tagtcgatgt      420

cagcagtcat ttgatttgtg gtcaattagt atcccagccg atattgcaag gttgcaaaac      480

tatgtttcag atgattttat aaaactgatt aatgcaataa atccagaaag taaagattca      540

atagccactg tattcaacgt ttatggcagc catgttctaa tgagtggtgt tatgggcggg      600

aaagctcacg tatccgcctc agcaaacaag ttaacactca cccaaaaatt tgagatgagt      660

acgattgttc aggctaaata cgaacaacta acatcacaac ttagcgtcga agacaaactt      720

aagtattcag aagccttcga tagtttcagc gaaagcggta gttacacata tgatattcta      780

ggcggtagcc cttctttagg ggcgcttgtt tttaaaaata actcgcaagg tagtagtgac      840

gataatttaa aaaattggat acagtctatt tccagtatgc cggtgttgac caagttcata      900

gaccaaacct cgttaatgcc tgtatggcta ttgtgtgaag acaagacaaa agccgatgca      960

ttaaaaaaat attacgataa tacatggtca aaatcgcaga tggccgttgc cagtctcaga     1020

gcaaactaca tcgacgagct gacatttgtc ctgggtgaca actcagatat ccctgctcca     1080

gtcggataca ctaaagttcc gattgatctt aattctgatg ctgggggtaa atatgtctat     1140

ctttgctatc atgaagcgca gttcacgcca gtaaatggca aacagccgat tgttgatatc     1200

caagtgctct atggtagcca gatgccagcg cctggatata taaaaattga tgttgatttg     1260

aatagcggtg ctggcgggga atttgtctac ctttcataca aaaagggaga accaacgtcg     1320

tccgatgtaa ttaataaaat caccgctgta tatggaaaaa atgagtacgt cccaacccca     1380

tatggatata aacaaattag cggtgacctt aatgctggag caggcggtga ctttgtttac     1440

ctctgtactt accaaggtgg tacagaataa                                      1470


<210>  24
<211>  1467
<212>  DNA
<213>  Serratia plymuthica

<400>  24
atgaaatttg aatcattaaa agttgaaaac ttattcagct catcattaac tcgcatcaat       60

gccattccac ttcctaacga cgccttacca ggcgtgggta ttattggctg tggttacaat      120

ccattcttgg cttatgcaga ttcgtcagct gtgctacacc ctgtactcga ttggtctaag      180

tcacagttcc acactgtaac catgaatggg cagacttatc agttgccgga aatacttaat      240

gcagtatggc taagcaacca gacgtactct tcagtatcag gtaaatcact tcagagttat      300

ttaactgaac tttctaatag tattaaggtg agtgggaatt atggtttttt tagtgcttca      360

gcaacaaatg aattcactga tagctcgttg agaaaatctg agaatgaatt tagtcgatgt      420

cagcagtcgt ttgatttgtg gtcaataagt gccccggcgg atatcgcaag gttacagaat      480

tacatttcag atgattttaa aaaactcatt agttcaattg atccaaataa tcaacaaaca      540

ttggcaacca ttttcaacgt atacggaagt catattttaa tgagtggtgt tatggggggg      600

aaagctcacg tttcagcatc agcgaacaag ctaactctca cgcaaaaatt cgaaatgagc      660

acgattgtac aggccaaata tgagcaactg acatcgcagc tcagcgctga agataaatta      720

aaatactctg aagcatttga aagtttcagt gagagtggta gctatactta cgatatttta      780

ggtggtagtc catcgctagg agcattggta tttaaggtta acaaccaaga cagtgacgac      840

aacctaagaa aatggataga ttcgatttca accatgcccg tacttactaa gtttatcgac      900

cagacatcgc tactaccggt gtggacgctg tgtgaagatc aggtaaaggc tgatgcgttg      960

aaaaattatt acaacaatac atggtctcaa ttgcaaatgg ctgtagcccg tctgagagcg     1020

aattacattg atgaagtgac ctttgttctt ggtgacaatt cagatatccc cgcacccgct     1080

ggttatacta aagtaccagt tgatctcaat tcaggcgcag ggggtaaatt tatttacctt     1140

tgctaccatg aagcacaatt tacccctgtt aactcaaaac aggctattgt tggtctccag     1200

gttctctatg gtaagcagga accggctcct gattattcga gaataaatat tgatttgaat     1260

agcggcgcaa atggagatga cgtctacctt tcatataaga aaggtgatgc aacttctaag     1320

gaggtaatta ataaaatcac cgctgtttat ggtaaagatc agtacgttcc gactccatat     1380

ggctataaac aaattcccgg cgaccttaac tctggtgcgg gaggtgattt tgtttatttc     1440

tgcacatacc agggtggaac cgaatag                                         1467


<210>  25
<211>  1470
<212>  DNA
<213>  Serratia quinivorans

<400>  25
atgaaaattg aatcatccaa ggttgaaggt ttgtttagct catcttttac tcgtgttaat       60

gctgtccctc tcccgagcga tactttaccc ggtgtaggta ttattggttg tggctacaat      120

ccatttttag cctatgcaga tgcttctgct gtcctgcatc ctattctcga ctggtcaaaa      180

tctcagtttc acgaaattac tatgaacggc cagcaatatc aattacctga tgtgcttcag      240

gcggtatggc tcagcaacca gtcttacgca tcggtaacgg ggaaatcgct ccaaagctat      300

ttaactgaat tggctaacag tattaaagta agtggtaact atggcttttt tagtgcctca      360

gccacaaatg aatttagcga cagttcattg agaaagtcag aaaatgagtt tagtcgatgt      420

cagcagtcat ttgacttgtg gtcaattagt atccctgccg atattgcaag gttgcaaaac      480

tatgtttcag atgattttat aaaactgatt aatgcaataa atccagaaag taaagattca      540

ttagccactg tattcaacgt ttatggcagc catgttctaa tgagtggtgt tatgggcggg      600

aaagctcacg tatccgcctc agcaaacaag ttaacactca cccaaaaatt tgagatgagt      660

acgattgttc aggctaaata cgaacaacta acatcacaac ttagcgtcga agacaaactt      720

aagtattcag aagccttcga tagtttcagc gaaagcggta gttacacata tgatattcta      780

ggcggtagcc cttctttagg ggcgctagtt tttaaagcta actcgcaggg tagtagtgac      840

gataatttaa aaagttggat acagtctatt tccagtatgc cggtgttgac caagttcata      900

gaccaaacct cgttaatgcc tgtatggcta ttgtgtgaag acaagacaaa agccgatgca      960

ttaaaaaaat attacgataa tacatggtca aaatcgcaga tggccgttgc cagtctcaga     1020

gcaaactaca tcgacgagct gacatttgtc ctgggtgaca actcagatat ccctgctcca     1080

gtcggataca ctaaagttcc gattgatctt aattctgatg ctgggggtaa atatgtctat     1140

ctttgctatc atgaagcgca gttcacgcca gtaaatggca aacagccgat tgttgatatc     1200

caagtgctct atggtagcca gatgccagcg cctggatata taaaaattga tgttgatttg     1260

aatagcggtg ctggcgggga atttgtctac ctttcataca aaaagggaga accaacgtcg     1320

tccgatgtaa ttaataaaat caccgctgta tatggaaaaa atgagtacgt cccaacccca     1380

tatggatata aacaaattag cggtgacctt aatgctggag caggcggtga ctttgtttac     1440

ctctgtactt atcaaggtgg gacagaataa                                      1470


<210>  26
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SproCRW sequence.

<400>  26
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  27
<211>  1467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli-optimized Sply sequence

<400>  27
atgaagttcg aaagccttaa ggtggagaac ctgttttcga gttcactgac ccggatcaat       60

gccattcccc ttcccaatga cgcattacct ggtgttggga ttattgggtg tggatacaac      120

ccttttctgg cctatgcgga ctcatctgct gtcttacatc ctgtgttgga ctggtcaaag      180

agtcaatttc atacggttac tatgaacgga caaacatatc aattgccgga aatccttaat      240

gccgtgtggt taagtaacca aacttactca tcggtctctg gaaaaagtct gcaaagttat      300

ctgaccgagc tttctaattc aatcaaagtt agtggaaatt atggattttt ttccgcgtct      360

gccacgaacg aattcaccga ttcatcctta agaaaatccg aaaatgaatt cagccgttgt      420

cagcagtctt tcgacttatg gtctataagc gcccctgcag atatagcacg cctgcagaat      480

tatataagtg acgattttaa gaagcttatt tcttcgatag atcccaacaa ccagcaaaca      540

ttggctacaa tattcaacgt gtatggatct catatactga tgagcggcgt catgggcgga      600

aaagcccacg tctcggcgag tgcgaataag cttaccttaa ctcagaaatt tgagatgtcc      660

acgattgttc aggcaaaata cgagcagctt acatcacaac tgtcggccga agacaagtta      720

aagtacagcg aggcgttcga atcattctcc gagtccggtt cctacaccta tgatatctta      780

ggaggttcac ccagtctggg agcattggtc ttcaaggtta acaaccagga ttctgacgat      840

aatttacgga agtggatcga ttcgatctcc accatgcctg tgttgacgaa atttatcgat      900

cagaccagtt tattgcctgt ctggacgctt tgcgaagatc aggtgaaagc cgacgccctg      960

aagaactact ataacaacac atggtcgcag ttgcagatgg ccgttgctag attacgggcc     1020

aactacattg acgaggtaac gtttgttctt ggcgataact ctgacatccc tgcgccagca     1080

gggtatacta aggtaccagt ggacctgaat tctggggcag gtggtaaatt catttatctt     1140

tgctatcatg aagcgcagtt tactcccgtg aactctaagc aagccatagt tggcttgcag     1200

gtcttgtacg ggaaacaaga accagcacct gattattcac gtattaacat agatctgaat     1260

tctggagcaa acggggatga tgtctacctt tcttataaaa agggggacgc cacttcgaaa     1320

gaagtcatca acaaaatcac tgcggtgtac gggaaggatc agtacgttcc cacaccgtat     1380

ggctacaagc aaattccggg ggacctgaat tcaggtgcgg gtggcgactt tgtctatttt     1440

tgtacttatc agggaggaac tgaataa                                         1467


<210>  28
<211>  1467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E. coli optimized SquiCRW sequence.

<400>  28
atgaaaattg aatcttcaaa ggtagaaggg ttattttcat catcctttac gcgtgtcaat       60

gcggttccgc tgccttcgga tactttgccc ggtgtaggca ttataggttg cgggtacaat      120

ccattcctgg cttatgcgga tgcgagcgcc gtgttgcacc ccatcctgga ttggtccaaa      180

agccagtttc atgagatcac gatgaatgga cagcaatacc agttaccaga tgttctgcaa      240

gctgtctggt tatcaaatca gtcctacgct tcggtaaccg gcaaatcatt gcaatcttac      300

cttacggaat tagctaactc aatcaaagta tcggggaact atggcttctt cagtgctagt      360

gccacaaacg agttctcaga ctcttcgttg cggaaaagcg aaaatgaatt tagccgctgc      420

caacaatctt tcgacctgtg gagcatatcc ataccggctg acattgcccg ccttcaaaat      480

tacgtttccg atgatttcat aaagttgatc aatgcgatta atcccgagag caaggactcc      540

ttagcgaccg tttttaatgt ctatggttcc cacgtcttaa tgagcggagt catgggaggc      600

aaagcccatg tcagtgcaag cgctaacaaa ttaactctga cccaaaaatt cgagatgtcc      660

acgatcgtgc aggcgaagta tgaacaactg acctctcaac tgagtgttga agacaagctg      720

aaatacagtg aggcctttga cagcttttcg gagagcggat cgtacactta tgatatactg      780

ggagggtcgc catctctggg agctcttgta ttcaaagcaa attcacaggg gagttccgac      840

gacaatctga agagttggat acagtccatt tcgtctatgc cagtcttaac gaaatttatc      900

gatcaaacta gcctgatgcc cgtgtggtta ctttgtgaag ataaaacaaa ggctgatgcc      960

ttaaaaaaat attatgataa cacttggagc aaatcgcaga tggcagttgc gagcttgcgt     1020

gcaaattaca ttgatgagtt gacatttgtt ttgggggaca atagcgacat accggcacca     1080

gtaggctaca caaaagtacc gattgatctg aacagtgatg ctggtgggaa gtacgtttat     1140

ctttgttatc atgaagccca atttaccccg gtgaacggta aacagccaat cgtggatatc     1200

caggtcctgt atggctctca aatgcccgca cctgggtata tcaagataga tgtggatttg     1260

aactctgggg caggaggtga gttcgtatac ttatcttaca agaaagggga accaacgagc     1320

tcagacgtta ttaacaaaat cactgcagtc tatggcaaaa atgagtatgt acccacgcct     1380

tacgggtata aacaaatttc tggcgatctg aacgctggcg caggcgggga tttcgtgtat     1440

ctttgcactt atcaaggtgg gacagag                                         1467


<210>  29
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW V8A L11I E. coli optimized sequence

<400>  29
atgaaaattg agagcagcaa ggctgaaggc attttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  30
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW Y188W E. coli optimized sequence

<400>  30
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctgggggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  31
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW Y188F E. coli optimized sequence

<400>  31
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctttgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  32
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW Y413W E. coli optimized sequence

<400>  32
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtgga ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  33
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW F428W E. coli optimized sequence

<400>  33
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atgggtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  34
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW Y430W E. coli optimized sequence

<400>  34
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtgg ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  35
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW S190P E. coli optimized sequence

<400>  35
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggccc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  36
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW V192Y E. coli optimized sequence

<400>  36
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc cattacttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  37
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C312L E. coli optimized sequence

<400>  37
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgctcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  38
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382A E. coli optimized sequence

<400>  38
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctggcctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  39
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382S E. coli optimized sequence

<400>  39
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgagttatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  40
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382T E. coli optimized sequence

<400>  40
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgacgtatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  41
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382M E. coli optimized sequence

<400>  41
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgatgtatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  42
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382G E. coli optimized sequence

<400>  42
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgggctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  43
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C382F E. coli optimized sequence

<400>  43
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgttttatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  44
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C482L E. coli optimized sequence

<400>  44
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgcttactt atcaaggggg gaccgaataa                                      1470


<210>  45
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW V398L E. coli optimized sequence

<400>  45
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cctggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  46
<211>  1476
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW P396-L-L-I397 E. coli optimized sequence

<400>  46
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccct gttgatcgtg     1200

gatattcagg ttctgtacgg ttctcagatg cctgcgccgg ggtatattaa gattgatgta     1260

gatctgaaca gcggggccgg tggtgaattt gtgtacctgt cctataaaaa aggggagccg     1320

acatcgtccg atgttattaa caaaatcacc gcggtttatg gaaagaatga atatgtaccg     1380

accccgtatg gctataaaca gatcagcggc gacctcaacg ctggggcggg gggtgacttc     1440

gtttatttgt gtacttatca aggggggacc gaataa                               1476


<210>  47
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW I397L E. coli optimized sequence

<400>  47
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccct ggtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  48
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW I397L V398L E. coli optimized sequence

<400>  48
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccct gttggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgtgtactt atcaaggggg gaccgaataa                                      1470


<210>  49
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW Y480L C482L E. coli optimized sequence:

<400>  49
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgttctg     1440

ttgcttactt atcaaggggg gaccgaataa                                      1470


<210>  50
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW C482L T483L E. coli optimized sequence:

<400>  50
atgaaaattg agagcagcaa ggtcgaaggc cttttctcct ctagcttcac acgggtgaat       60

gctgtgccgc ttccgagtga caccctgccg ggtgtaggta tcattggttg cggttataat      120

ccgtttctgg cgtatgccga cgctagcgcc gttctgcacc cgattctcga ttggtcaaag      180

tcacaattta atgaaattac catgaatggc cagcagtacc aacttcctga tgtccttcaa      240

gcagtgtggt taagcaacca gtcgtacgcc tctgtgacag gcaaatcgct ccagtcatac      300

cttactgaac tcgcgaactc tatcaaagtc tctggcaatt atggattttt ttcggcctct      360

gccacgaacg aatttagcga ctcgtcactg cgcaaatcag aaaacgaatt ttcacgctgc      420

cagcaaagtt ttgatttatg gtctatttcg atccctgctg atattgcccg cctccagaac      480

tacgtgtcgg atgactttat taaactcatc aatgcaatta atccagagtc caaagattct      540

atcgccaccg tgtttaacgt ctacgggtcc catgttttaa tgtctggtgt aatggggggg      600

aaagcgcacg ttagcgcctc ggctaataag cttaccctga cccagaaatt tgaaatgtcc      660

accattgtac aagctaaata cgaacagctt accagtcagc tgagcgtaga agacaagctg      720

aaatatagtg aagcgttcga ttcgttttcg gagtctggct cgtataccta cgatatcctg      780

ggcggttcgc cgtccttagg ggctttagtt ttcaaaaaca atagtcaggg ctcgtctgac      840

gataacctta agaactggat ccaatctatt agctcgatgc cagtacttac caaattcatc      900

gaccagacaa gcctgatgcc cgtttggctg ttgtgcgagg ataaaaccaa agccgatgcg      960

ctgaaaaaat attacgataa cacctggtcc aagagccaaa tggccgtcgc aagcctgcgc     1020

gcgaactaca tcgatgaact gaccttcgtc ctcggtgata attctgacat tcctgcgccg     1080

gtaggttata cgaaagtccc gatcgatctt aattcggatg ccggcggtaa atacgtttat     1140

ctgtgctatc acgaagccca gtttaccccg gtgaacggca aacagcccat cgtggatatt     1200

caggttctgt acggttctca gatgcctgcg ccggggtata ttaagattga tgtagatctg     1260

aacagcgggg ccggtggtga atttgtgtac ctgtcctata aaaaagggga gccgacatcg     1320

tccgatgtta ttaacaaaat caccgcggtt tatggaaaga atgaatatgt accgaccccg     1380

tatggctata aacagatcag cggcgacctc aacgctgggg cggggggtga cttcgtttat     1440

ttgcttctgt atcaaggggg gaccgaataa                                      1470


<210>  51
<211>  1467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SplyCRW C481L E. coli optimized sequence:

<400>  51
atgaagttcg aaagccttaa ggtggagaac ctgttttcga gttcactgac ccggatcaat       60

gccattcccc ttcccaatga cgcattacct ggtgttggga ttattgggtg tggatacaac      120

ccttttctgg cctatgcgga ctcatctgct gtcttacatc ctgtgttgga ctggtcaaag      180

agtcaatttc atacggttac tatgaacgga caaacatatc aattgccgga aatccttaat      240

gccgtgtggt taagtaacca aacttactca tcggtctctg gaaaaagtct gcaaagttat      300

ctgaccgagc tttctaattc aatcaaagtt agtggaaatt atggattttt ttccgcgtct      360

gccacgaacg aattcaccga ttcatcctta agaaaatccg aaaatgaatt cagccgttgt      420

cagcagtctt tcgacttatg gtctataagc gcccctgcag atatagcacg cctgcagaat      480

tatataagtg acgattttaa gaagcttatt tcttcgatag atcccaacaa ccagcaaaca      540

ttggctacaa tattcaacgt gtatggatct catatactga tgagcggcgt catgggcgga      600

aaagcccacg tctcggcgag tgcgaataag cttaccttaa ctcagaaatt tgagatgtcc      660

acgattgttc aggcaaaata cgagcagctt acatcacaac tgtcggccga agacaagtta      720

aagtacagcg aggcgttcga atcattctcc gagtccggtt cctacaccta tgatatctta      780

ggaggttcac ccagtctggg agcattggtc ttcaaggtta acaaccagga ttctgacgat      840

aatttacgga agtggatcga ttcgatctcc accatgcctg tgttgacgaa atttatcgat      900

cagaccagtt tattgcctgt ctggacgctt tgcgaagatc aggtgaaagc cgacgccctg      960

aagaactact ataacaacac atggtcgcag ttgcagatgg ccgttgctag attacgggcc     1020

aactacattg acgaggtaac gtttgttctt ggcgataact ctgacatccc tgcgccagca     1080

gggtatacta aggtaccagt ggacctgaat tctggggcag gtggtaaatt catttatctt     1140

tgctatcatg aagcgcagtt tactcccgtg aactctaagc aagccatagt tggcttgcag     1200

gtcttgtacg ggaaacaaga accagcacct gattattcac gtattaacat agatctgaat     1260

tctggagcaa acggggatga tgtctacctt tcttataaaa agggggacgc cacttcgaaa     1320

gaagtcatca acaaaatcac tgcggtgtac gggaaggatc agtacgttcc cacaccgtat     1380

ggctacaagc aaattccggg ggacctgaat tcaggtgcgg gtggcgactt tgtctatttt     1440

ttgacttatc agggaggaac tgaataa                                         1467


<210>  52
<211>  1467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SplyCRW Y479L C481L E. coli optimized sequence:

<400>  52
atgaagttcg aaagccttaa ggtggagaac ctgttttcga gttcactgac ccggatcaat       60

gccattcccc ttcccaatga cgcattacct ggtgttggga ttattgggtg tggatacaac      120

ccttttctgg cctatgcgga ctcatctgct gtcttacatc ctgtgttgga ctggtcaaag      180

agtcaatttc atacggttac tatgaacgga caaacatatc aattgccgga aatccttaat      240

gccgtgtggt taagtaacca aacttactca tcggtctctg gaaaaagtct gcaaagttat      300

ctgaccgagc tttctaattc aatcaaagtt agtggaaatt atggattttt ttccgcgtct      360

gccacgaacg aattcaccga ttcatcctta agaaaatccg aaaatgaatt cagccgttgt      420

cagcagtctt tcgacttatg gtctataagc gcccctgcag atatagcacg cctgcagaat      480

tatataagtg acgattttaa gaagcttatt tcttcgatag atcccaacaa ccagcaaaca      540

ttggctacaa tattcaacgt gtatggatct catatactga tgagcggcgt catgggcgga      600

aaagcccacg tctcggcgag tgcgaataag cttaccttaa ctcagaaatt tgagatgtcc      660

acgattgttc aggcaaaata cgagcagctt acatcacaac tgtcggccga agacaagtta      720

aagtacagcg aggcgttcga atcattctcc gagtccggtt cctacaccta tgatatctta      780

ggaggttcac ccagtctggg agcattggtc ttcaaggtta acaaccagga ttctgacgat      840

aatttacgga agtggatcga ttcgatctcc accatgcctg tgttgacgaa atttatcgat      900

cagaccagtt tattgcctgt ctggacgctt tgcgaagatc aggtgaaagc cgacgccctg      960

aagaactact ataacaacac atggtcgcag ttgcagatgg ccgttgctag attacgggcc     1020

aactacattg acgaggtaac gtttgttctt ggcgataact ctgacatccc tgcgccagca     1080

gggtatacta aggtaccagt ggacctgaat tctggggcag gtggtaaatt catttatctt     1140

tgctatcatg aagcgcagtt tactcccgtg aactctaagc aagccatagt tggcttgcag     1200

gtcttgtacg ggaaacaaga accagcacct gattattcac gtattaacat agatctgaat     1260

tctggagcaa acggggatga tgtctacctt tcttataaaa agggggacgc cacttcgaaa     1320

gaagtcatca acaaaatcac tgcggtgtac gggaaggatc agtacgttcc cacaccgtat     1380

ggctacaagc aaattccggg ggacctgaat tcaggtgcgg gtggcgactt tgtccttttt     1440

ttgacttatc agggaggaac tgaataa                                         1467


<210>  53
<211>  1467
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SplyCRW C481L T482L E. coli optimized sequence:

<400>  53
atgaagttcg aaagccttaa ggtggagaac ctgttttcga gttcactgac ccggatcaat       60

gccattcccc ttcccaatga cgcattacct ggtgttggga ttattgggtg tggatacaac      120

ccttttctgg cctatgcgga ctcatctgct gtcttacatc ctgtgttgga ctggtcaaag      180

agtcaatttc atacggttac tatgaacgga caaacatatc aattgccgga aatccttaat      240

gccgtgtggt taagtaacca aacttactca tcggtctctg gaaaaagtct gcaaagttat      300

ctgaccgagc tttctaattc aatcaaagtt agtggaaatt atggattttt ttccgcgtct      360

gccacgaacg aattcaccga ttcatcctta agaaaatccg aaaatgaatt cagccgttgt      420

cagcagtctt tcgacttatg gtctataagc gcccctgcag atatagcacg cctgcagaat      480

tatataagtg acgattttaa gaagcttatt tcttcgatag atcccaacaa ccagcaaaca      540

ttggctacaa tattcaacgt gtatggatct catatactga tgagcggcgt catgggcgga      600

aaagcccacg tctcggcgag tgcgaataag cttaccttaa ctcagaaatt tgagatgtcc      660

acgattgttc aggcaaaata cgagcagctt acatcacaac tgtcggccga agacaagtta      720

aagtacagcg aggcgttcga atcattctcc gagtccggtt cctacaccta tgatatctta      780

ggaggttcac ccagtctggg agcattggtc ttcaaggtta acaaccagga ttctgacgat      840

aatttacgga agtggatcga ttcgatctcc accatgcctg tgttgacgaa atttatcgat      900

cagaccagtt tattgcctgt ctggacgctt tgcgaagatc aggtgaaagc cgacgccctg      960

aagaactact ataacaacac atggtcgcag ttgcagatgg ccgttgctag attacgggcc     1020

aactacattg acgaggtaac gtttgttctt ggcgataact ctgacatccc tgcgccagca     1080

gggtatacta aggtaccagt ggacctgaat tctggggcag gtggtaaatt catttatctt     1140

tgctatcatg aagcgcagtt tactcccgtg aactctaagc aagccatagt tggcttgcag     1200

gtcttgtacg ggaaacaaga accagcacct gattattcac gtattaacat agatctgaat     1260

tctggagcaa acggggatga tgtctacctt tcttataaaa agggggacgc cacttcgaaa     1320

gaagtcatca acaaaatcac tgcggtgtac gggaaggatc agtacgttcc cacaccgtat     1380

ggctacaagc aaattccggg ggacctgaat tcaggtgcgg gtggcgactt tgtctatttt     1440

ttgctttatc agggaggaac tgaataa                                         1467


<210>  54
<211>  1470
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SproCRW V8A L11I maize-optimized sequence

<400>  54
atgaagattg agtccagcaa ggccgagggc atcttctcgt cgtcgttcac ccgcgttaat       60

gccgttccac tcccatcgga tacactgccg ggcgtcggga tcattggctg cgggtacaac      120

cccttcctcg cttacgccga cgcttcggcg gtgctccacc cgattctgga ttggtctaag      180

tcacagttca acgagatcac catgaacggc cagcagtacc agctccccga cgttctgcag      240

gctgtgtggc tctcaaacca gtcctacgcc agcgtcaccg gcaagtcgct gcagtcttac      300

ctcacggagc tggccaactc tatcaaggtg tcaggcaatt acgggttctt ctccgcgagc      360

gctactaacg agttcagcga ctccagcctc cgcaagtccg agaatgagtt cagcaggtgc      420

cagcagtcgt tcgacctgtg gtcgatctct attccagccg atattgcgcg gctccagaac      480

tacgtctccg acgatttcat caagctgatt aacgcgatca atcctgagtc aaaggactcc      540

atcgctaccg tgttcaacgt ctacggctct cacgtgctca tgtcaggggt catgggcggg      600

aaggctcatg tgtcagcttc cgcgaataag ctcaccctga cgcagaagtt cgagatgtcc      660

accatcgttc aggccaagta cgagcagctc acgagccagc tgtcggtgga ggacaagctc      720

aagtactccg aggcgttcga tagcttctcg gagtctggct catacaccta cgacatcctc      780

ggcggctccc cgagcctggg cgcgctggtg ttcaagaaca attcgcaggg gtcgtctgac      840

gataacctga agaattggat tcagtctatc tcatccatgc cagtcctcac aaagttcatc      900

gatcagactt ccctgatgcc tgtttggctc ctgtgcgagg acaaaaccaa ggctgatgcc      960

ctcaagaaat actacgacaa cacttggtcg aagtctcaga tggcggtcgc ttccctgcgc     1020

gcgaactaca tcgatgagct gaccttcgtg ctgggcgaca atagcgatat tccggctccc     1080

gttggctaca cgaaggtgcc gatcgacctc aactcggatg ccggcggcaa gtatgtgtac     1140

ctgtgctacc atgaggcgca gttcacgcca gtcaatggca agcagcctat tgtcgacatc     1200

caggttctct acggctccca gatgccagct cctggataca taaagattga cgtggatctc     1260

aactcgggcg ccggcgggga gttcgtttac ctgagctaca agaagggcga gcccacaagc     1320

tcggacgtca taaacaagat cactgcggtt tacggcaaga acgagtatgt gccgacaccc     1380

tacggctaca agcagatcag cggggatctg aatgcggggg cgggcggcga tttcgtttac     1440

ctctgcacct accagggcgg gaccgagtga                                      1470


