                         SEQUENCE LISTING

<110>  Kutyavin, Igor
 
<120>  THERMOKINETICALLY BALANCED ISOTHERMAL AMPLIFICATION OF NUCLEIC 
       ACID SEQUENCES

<130>  0067898-014WO0

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 1

<400>  1
ggttcctatt gggcttgct                                                    19


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 2

<400>  2
ccaccctcat tttcagggat                                                   20


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET labeled primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-(BHQ1) label

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  FAM-U

<400>  3
ccaccctcat ttncagggat                                                   20


<210>  4
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment of target sequence M13mp18

<400>  4
ggttcctatt gggcttgcta tccctgaaaa tgagggtgg                              39


<210>  5
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  first modified fragment of target sequence M13mp18

<400>  5
ggttcctatt gggcttgcta atccctgaaa atgagggtgg                             40


<210>  6
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second modified fragment of target sequence M13mp18

<400>  6
ggttcctatt gggcttgcta aaatccctga aaatgagggt gg                          42


<210>  7
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  third modified fragment of target sequence M13mp18

<400>  7
ggttcctatt gggcttgcta aaaaatccct gaaaatgagg gtgg                        44


<210>  8
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fourth modified fragment of target sequence M13mp18

<400>  8
ggttcctatt gggcttgcta aaaaaaaatc cctgaaaatg agggtgg                     47


<210>  9
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fifth modified fragment of target sequence M13mp18

<400>  9
ggttcctatt gggcttgcta tatatatatc cctgaaaatg agggtgg                     47


<210>  10
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sixth modified fragment of target sequence M13mp18

<400>  10
ggttcctatt gggcttgcta aaaaaaaaaa aatccctgaa aatgagggtg g                51


<210>  11
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  seventh modified fragment of target sequence M13mp18

<400>  11
ggttcctatt gggcttgcta tatatatata tatccctgaa aatgagggtg g                51


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  eigth modified fragment of target sequence M13mp18

<400>  12
ggttcctatt gggcttgctg atccctgaaa atgagggtgg                             40


<210>  13
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ninth modified fragment of target sequence M13mp18

<400>  13
ggttcctatt gggcttgctg gatccctgaa aatgagggtg g                           41


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide-modified primer


<220>
<221>  modified_base
<222>  (1)..(34)
<223>  A at positions 1, 5, 11, 16 and 18 are 2,6-diaminopurine; T at 
       positions 3, 7, 9, 10, 15, 19, 23, 24, 27, 29, 30 are 5-propynyl 
       2'-deoxyuridine; and C at positions 2, 4, 12, 17, 25, 26, 34 are 
       5-propynyl 2'-deoxycytidine

<400>  14
actcagtgtt acggtacatg ggttcctatt gggc                                   34


<210>  15
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FRET probe


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM label

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  (BHQ1)-3' label

<400>  15
ttcagggata gcaag                                                        15


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 16

<400>  16
tcagagccac caccctcatt                                                   20


<210>  17
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second fragment of target sequence M13mp18

<400>  17
ggtgacgaaa ctcagtgtta cggtacatgg gttcctattg ggcttgctat ccctgaaaat       60

gagggtggtg gctctgaggg tggcgg                                            86


