                         SEQUENCE LISTING

<110>  Casebia Therapeutics
       Cost, Gregory J.
       Uenishi, Gene I.
 
<120>  Compositions and Methods for NHEJ-Mediated Genome Editing

<130>  052984-528001WO

<150>  62/752,959
<151>  2018-10-30

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  1511
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  0-cut SA-GFP expression cassette

<400>  1
cctgcactac cagagctaac tcactgacct cttctcttcc tcccacaggg cctcgagaga       60

tctggcagcg gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg      120

gaggagaacc ctggacctac cggtatgcct gaaccctcta agtctgctcc cgccccgaaa      180

aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc      240

agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac      300

accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag      360

cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc      420

agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg      480

tccgagggta ctaaggccgt caccaagtac accagcgcta aggacatggt gagcaagggc      540

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      600

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      660

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg      720

acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc      780

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttctttaa ggatgacggc      840

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag      900

ctgaagggca tcgactttaa agaggacggg aacatcctgg ggcacaagct ggagtacaac      960

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac     1020

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     1080

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     1140

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     1200

accgccgccg ggatcactct cggcatggac gagctgtaca agtaacatat ggctgatcag     1260

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct     1320

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc     1380

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     1440

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctacc tgcactacca     1500

gagctaactc a                                                          1511


<210>  2
<211>  1511
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  1-cut SA-GFP expression cassette

<400>  2
cctatcctgt ccctagtggc cccctgacct cttctcttcc tcccacaggg cctcgagaga       60

tctggcagcg gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg      120

gaggagaacc ctggacctac cggtatgcct gaaccctcta agtctgctcc cgccccgaaa      180

aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc      240

agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac      300

accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag      360

cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc      420

agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg      480

tccgagggta ctaaggccgt caccaagtac accagcgcta aggacatggt gagcaagggc      540

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      600

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      660

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg      720

acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc      780

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttctttaa ggatgacggc      840

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag      900

ctgaagggca tcgactttaa agaggacggg aacatcctgg ggcacaagct ggagtacaac      960

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac     1020

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     1080

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     1140

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     1200

accgccgccg ggatcactct cggcatggac gagctgtaca agtaacatat ggctgatcag     1260

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct     1320

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc     1380

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     1440

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctacc tgcactacca     1500

gagctaactc a                                                          1511


<210>  3
<211>  1511
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  2-cut SA-GFP expression cassette

<400>  3
cctatcctgt ccctagtggc cccctgacct cttctcttcc tcccacaggg cctcgagaga       60

tctggcagcg gaggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg      120

gaggagaacc ctggacctac cggtatgcct gaaccctcta agtctgctcc cgccccgaaa      180

aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc      240

agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac      300

accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag      360

cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc      420

agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg      480

tccgagggta ctaaggccgt caccaagtac accagcgcta aggacatggt gagcaagggc      540

gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc      600

cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg      660

aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg      720

acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc      780

aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttctttaa ggatgacggc      840

aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag      900

ctgaagggca tcgactttaa agaggacggg aacatcctgg ggcacaagct ggagtacaac      960

tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac     1020

ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag     1080

aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag     1140

tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg     1200

accgccgccg ggatcactct cggcatggac gagctgtaca agtaacatat ggctgatcag     1260

cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct     1320

tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc     1380

attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     1440

aggattggga agacaatagc aggcatgctg gggatgcggt gggctctacc tatcctgtcc     1500

ctagtggccc c                                                          1511


<210>  4
<211>  1892
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  0-cut SA-GFP scAAV

<400>  4
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggacg      120

cgtaggccta agcttggtac cggatccact agtaacggcc gccagtgtgc tggaattcgc      180

ccttctttga gttagctctg gtagtgcagg tagagcccac cgcatcccca gcatgcctgc      240

tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat      300

gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag      360

tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac      420

tagaaggcac agtcgaggct gatcagccat atgttacttg tacagctcgt ccatgccgag      480

agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg      540

gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc      600

gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat      660

gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata      720

gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttcc cgtcctcttt      780

aaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc      840

ggcgcgggtc ttgtagttgc cgtcatcctt aaagaagatg gtgcgctcct ggacgtagcc      900

ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa      960

gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc     1020

ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc     1080

ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac     1140

cccggtgaac agctcctcgc ccttgctcac catgtcctta gcgctggtgt acttggtgac     1200

ggccttagta ccctcggaca cggcgtgctt ggccaactcc ccaggcagca gcaggcgcac     1260

ggccgtctgg atctccctgg aggtgatggt cgagcgcttg ttgtaatgcg ccaggcggga     1320

agcctcacct gcgatgcgct cgaaaatgtc gttcacaaac gaattcatga tgcccatggc     1380

cttggacgaa atgccggtgt cagggtggac ctgcttcaga accttgtaca catagatgga     1440

atagctctcc ttgcggctgc gcttgcgctt cttgccgcct ttcttctgcg ccttagtcac     1500

cgccttcttg gagccctttt tcggggcggg agcagactta gagggttcag gcataccggt     1560

aggtccaggg ttctcctcca cgtctccagc ctgcttcagc aggctgaagt tagtagctcc     1620

gcttcctccg ctgccagatc tctcgaggcc ctgtgggagg aagagaagag gtcagtgagt     1680

tagctctggt agtgcaggta ccaagggcga attctgcaga tatccatcac actggcttaa     1740

ttaagggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc     1800

gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc     1860

agtgagcgag cgagcgcgca gctgcctgca gg                                   1892


<210>  5
<211>  1895
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  2-cut SA-GFP scAAV

<400>  5
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac       60

gcccgggctt tgcccgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggacg      120

cgtaggccta agcttggtac cggatccact agtaacggcc gccagtgtgc tggaattcgc      180

ccttggtacc tatcctgtcc ctagtggccc cctgacctct tctcttcctc ccacagggcc      240

tcgagagatc tggcagcgga ggaagcggag ctactaactt cagcctgctg aagcaggctg      300

gagacgtgga ggagaaccct ggacctaccg gtatgcctga accctctaag tctgctcccg      360

ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc      420

gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc      480

accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca      540

ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca      600

tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc      660

acgccgtgtc cgagggtact aaggccgtca ccaagtacac cagcgctaag gacatggtga      720

gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg      780

taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc      840

tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga      900

ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg      960

acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttctttaagg     1020

atgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc     1080

gcatcgagct gaagggcatc gactttaaag aggacgggaa catcctgggg cacaagctgg     1140

agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca     1200

aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact     1260

accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga     1320

gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg     1380

agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taacatatgg     1440

ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt     1500

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     1560

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     1620

caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctaccta     1680

tcctgtccct agtggcccca agcttaaggg cgaattctgc agatatccat cacactggct     1740

taattaaggg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg     1800

ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc     1860

ctcagtgagc gagcgagcgc gcagctgcct gcagg                                1895


<210>  6
<211>  1613
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  2-cut SA-tNGFR scAAV

<400>  6
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggacg      120

cgtaggccta agcttggtac cggatccact agtaacggcc gccagtgtgc tggaattcgc      180

ccttggtacc tatcctgtcc ctagtggccc cctgacctct tctcttcctc ccacagggcc      240

tcgagagatc tggcagcgga ggaagcggag ctactaactt cagcctgctg aagcaggctg      300

gagacgtgga ggagaaccct ggacctaccg gtatgggggc aggtgccacc ggccgcgcca      360

tggacgggcc gcgcctgctg ctgttgctgc ttctgggggt gtcccttgga ggtgccaagg      420

aggcatgccc cacaggcctg tacacacaca gcggtgagtg ctgcaaagcc tgcaacctgg      480

gcgagggtgt ggcccagcct tgtggagcca accagaccgt gtgtgagccc tgcctggaca      540

gcgtgacgtt ctccgacgtg gtgagcgcga ccgagccgtg caagccgtgc accgagtgcg      600

tggggctcca gagcatgtcg gcgccgtgcg tggaggccga cgacgccgtg tgccgctgcg      660

cctacggcta ctaccaggat gagacgactg ggcgctgcga ggcgtgccgc gtgtgcgagg      720

cgggctcggg cctcgtgttc tcctgccagg acaagcagaa caccgtgtgc gaggagtgcc      780

ccgacggcac gtattccgac gaggccaacc acgtggaccc gtgcctgccc tgcaccgtgt      840

gcgaggacac cgagcgccag ctccgcgagt gcacacgctg ggccgacgcc gagtgcgagg      900

agatccctgg ccgttggatt acacggtcca cacccccaga gggctcggac agcacagccc      960

ccagcaccca ggagcctgag gcacctccag aacaagacct catagccagc acggtggcag     1020

gtgtggtgac cacagtgatg ggcagctccc agcccgtggt gacccgaggc accaccgaca     1080

acctcatccc tgtctattgc tccatcctgg ctgctgtggt tgtgggcctt gtggcctaca     1140

tagccttctg acatatggct gatcagcctc gactgtgcct tctagttgcc agccatctgt     1200

tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc     1260

ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg     1320

tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga     1380

tgcggtgggc tctacctatc ctgtccctag tggccccaag cttaagggcg aattctgcag     1440

atatccatca cactggctta attaagggcc gcaggaaccc ctagtgatgg agttggccac     1500

tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc     1560

gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc agg            1613


<210>  7
<211>  3591
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  HDR-TI SA-GFP ssAAV

<400>  7
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc       60

gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca      120

actccatcac taggggttcc tgcggccaat tcagtcgata actataacgg tcctaaggta      180

gcgatttaaa tacgcgctct cttaaggtag ccccgggacg cgtcaattga gatctggatc      240

cggtacctgc actaccagag ctaactcatg ctttctctga ccagcattct ctcccctggg      300

cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac ctctacggct ggcccagatc      360

cttccctgcc gcctccttca ggttccgtct tcctccactc cctcttcccc ttgctctctg      420

ctgtgttgct gcccaaggat gctctttccg gagcacttcc ttctcggcgc tgcaccacgt      480

gatgtcctct gagcggatcc tccccgtgtc tgggtcctct ccgggcatct ctcctccctc      540

acccaacccc atgccgtctt cactcgctgg gttccctttt ccttctcctt ctggggcctg      600

tgccatctct cgtttcttag gatggccttc tccgacggat gtctcccttg cgtcccgcct      660

ccccttcttg taggcctgca tcatcaccgt ttttctggac aaccccaaag taccccgtct      720

ccctggcttt agccacctct ccatcctctt gctttctttg cctggacacc ccgttctcct      780

gtggattcgg gtcacctctc actcctttca tttgggcagc tcccctaccc cccttacctc      840

tctagtctgt gctagctctt ccagccccct gtcatggcat cttccagggg tccgagagct      900

cagctagtct tcttcctcca acccgggccc ctatgtccac ttcaggacag catgtttgct      960

gcctccaggg atcctgtgtc cccgagctgg gaccacctta tattcccagg gccggttaat     1020

gtggctctgg ttctgggtac ttttatctgt cccctccacc ccacagtggg gccactaggg     1080

acagctgacc tcttctcttc ctcccacagg gctctggcag cggaggaagc ggagctacta     1140

actttagtct gcttaatcaa gctggagacg tggaggagaa ccctggacct accggtatgc     1200

ctgaaccctc taagtctgct cccgccccga aaaagggctc caagaaggcg gtgactaagg     1260

cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat tccatctatg     1320

tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag gccatgggca     1380

tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct tcccgcctgg     1440

cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc gtgcgcctgc     1500

tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc gtcaccaagt     1560

acaccagcgc taaggacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca     1620

tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg     1680

agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc     1740

ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct     1800

accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc     1860

aggagcgcac catcttcttt aaggatgacg gcaactacaa gacccgcgcc gaggtgaagt     1920

tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttt aaagaggacg     1980

ggaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg     2040

ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg     2100

gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc     2160

tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga     2220

agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg     2280

acgagctgta caagtaacat atggctgatc agcctcgact gtgccttcta gttgccagcc     2340

atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt     2400

cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct     2460

ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc     2520

tggggatgcg gtgggctcta tgattggtga cagaaaagcc ccatccttag gcctcctcct     2580

tcctagtctc ctgatattgg gtctaacccc cacctcctgt taggcagatt ccttatctgg     2640

tgacacaccc ccatttcctg gagccatctc tctccttgcc agaacctcta aggtttgctt     2700

acgatggagc cagagaggat cctgggaggg agagcttggc agggggtggg agggaagggg     2760

gggatgcgtg acctgcccgg ttctcagtgg ccaccctgcg ctaccctctc ccagaacctg     2820

agctgctctg acgcggctgt ctggtgcgtt tcactgatcc tggtgctgca gcttccttac     2880

acttcccaag aggagaagca gtttggaaaa acaaaatcag aataagttgg tcctgagttc     2940

taactttggc tcttcacctt tctagtcccc aatttatatt gttcctccgt gcgtcagttt     3000

tacctgtgag ataaggccag tagccagccc cgtcctggca gggctgtggt gaggaggggg     3060

gtgtccgtgt ggaaaactcc ctttgtgaga atggtgcgtc ctaggtgttc accaggtcgt     3120

ggccgcctct actccctttc tctttctcca tccttctttc cttaaagagt ccccagtgct     3180

atctgggaca tattcctccg cccagagcag ggtcccgctt ccctaaggcc ctgctctggg     3240

cttctgggtt tgagtccttg gcaagcccag gagaggcgct caggcttccc tgtccccctt     3300

cctcgtccac catctcatgc ccctggctct cctgcccctt ccctacaggg gttcctgcac     3360

taccagagct aactcaaagc ttctagatat cctctcttaa ggtagcatcg agatttaaat     3420

tagggataac agggtaatgg cgcgggccgc aggaacccct agtgatggag ttggccactc     3480

cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg     3540

gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g              3591


<210>  8
<211>  100
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 sgRNA

<400>  8
ggggccacua gggacaggau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  9
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Wild-type AAVS1 protospacer with PAM

<400>  9
ggggccacta gggacaggat tgg                                               23


<210>  10
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Primer 1 AAVS1-outsideF

<400>  10
cggaactctg ccctctaacg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  Primer 2 AAVS1-outsideR

<400>  11
ctgggatacc ccgaagagtg                                                   20


<210>  12
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 TIDE-4R

<400>  12
cctctccatc ctcttgcttt ctttg                                             25


<210>  13
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 TIDE-4F

<400>  13
aactgcttct cctcttggga agt                                               23


<210>  14
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  IntAmp1

<400>  14
gatgggggtg ttctgctgg                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  IntAmp2

<400>  15
cgtaaacggc cacaagttca                                                   20


<210>  16
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 1

<400>  16
ggggccacta gggagaggat agg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 2

<400>  17
ggggccacta gggccaggat agg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 3

<400>  18
ggggccacta ggaacaggat agg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 4

<400>  19
gggaccacta gggacaggat agg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 5

<400>  20
ggagccacta gggacaggat agg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 6

<400>  21
gaggccacta gggacaggat agg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 7

<400>  22
tgggccacta gggacaggat agg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 8

<400>  23
caggccacta gggacaggat agg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 9

<400>  24
taggccacta gggacaggat agg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 10

<400>  25
ctagccacta gggacaggat agg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 11

<400>  26
tatgccacta gggacaggat agg                                               23


<210>  27
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 12

<400>  27
gatgccacta gggacaggat agg                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVS1 DAP with PAM 13

<400>  28
gtagccacta gggacaggat agg                                               23


