                         SEQUENCE LISTING

<110>  Rhode Island Hospital
 
<120>  MALARIA ANTIGENS ON THE SURFACE OF ERYTHROCYTES AND MEROZOITES 
       AND PROTECTIVE ANTIBODIES

<130>  21486-639001WO

<150>  62/757,151
<151>  2018-11-07

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfEMMA1 fragment

<400>  1

Asp Asn Val Asn Asn Asn Asn Asn Lys Glu Ser Cys Asp Asn Ile Lys 
1               5                   10                  15      


His Met Arg Thr Lys Ser Leu Asn Phe Val Ser Arg Glu Ser Tyr Gly 
            20                  25                  30          


Glu His Lys Ser Leu Asp Val Tyr Gln Glu Cys Tyr Val Lys Asn Asn 
        35                  40                  45              


Lys Leu Ile Asn Lys Val Asn Asp Lys Lys Tyr Glu Asp Asn Asn Asn 
    50                  55                  60                  


Ser Tyr Leu Asn Glu Asp Asp Asn Ala Ser Met Gln Phe Tyr Glu Glu 
65                  70                  75                  80  


Thr Asn Ser Asn Pro Tyr Ile Val Asp Gln Glu Asn Asn Met Lys Asn 
                85                  90                  95      


Tyr Val Asn Asn Val Leu Tyr Asn Asn Asn Ser Asn Tyr Tyr Val Asp 
            100                 105                 110         


Ser Lys Asn Tyr Asp Lys Ser Lys Glu Asn Ala Glu Asn Lys Ser Asp 
        115                 120                 125             


Asp Ile Leu Asn Asn Glu Asn Ile His Thr Leu Lys Asp Gln Lys Lys 
    130                 135                 140                 


Lys Ile Gln Asn Asn Asn Glu Phe Ile Ser Glu Gln Ala Asp Ile Glu 
145                 150                 155                 160 


Asn Ile Arg Asn Ser Gln Glu Glu Val Tyr Glu Lys Glu His Glu Pro 
                165                 170                 175     


Leu Trp Val Ile Asn Ala Ser Asn Glu Glu Lys Lys Ser Tyr Glu Glu 
            180                 185                 190         


Leu Ile Tyr Ser Asp Met Ser Ser Asn Arg Val Thr Lys Asn Lys Tyr 
        195                 200                 205             


Ser Asp Met Asn Asn Val Glu Val Leu Leu Asn Glu Asp Asn Leu Leu 
    210                 215                 220                 


Thr Thr Glu Lys Tyr Lys Val Gln Leu Glu Lys Glu Asn Lys 
225                 230                 235             


<210>  2
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Frag1 nucleotide fragment

<400>  2
gataatgtta ataataataa taataaagaa agttgtgata atattaaaca tatgagaaca       60

aaaagtttaa attttgtaag tagagaatcc tatggcgaac ataaaagtct agatgtttac      120

caggaatgtt atgtaaaaaa taataaactt attaataagg taaatgataa aaaatatgag      180

gacaataata attcctatct taatgaagat gataacgcta gtatgcaatt ttatgaagaa      240

actaatagta atccatatat tgtagaccag gaaaataata tgaaaaatta tgtcaataat      300

gttttatata acaacaatag caattattat gttgattcaa agaattatga taaatctaaa      360

gagaatgcag aaaataaatc agatgatata ttaaataatg aaaatataca taccttaaaa      420

gatcaaaaaa agaaaataca aaataataat gaattcatta gtgaacaggc tgatatagaa      480

aatataagaa attctcaaga agaagtatat gagaaagaac acgaaccttt gtgggtaata      540

aatgcatcta atgaagaaaa gaaatcatat gaagaattga tatacagcga tatgtcatct      600

aatcgtgtta cgaaaaataa atatagtgat atgaataatg ttgaggtatt attaaatgaa      660

gataatttat taactactga aaaatacaag gtgcaattag aaaaagaaaa taaa            714


<210>  3
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfEMMA1 fragment

<400>  3

Asn Arg Val Thr Lys Asn Lys Tyr Ser Asp Met Asn Asn Val Glu Val 
1               5                   10                  15      


Leu Leu Asn Glu Asp Asn Leu Leu Thr Thr Glu Lys Tyr Lys Val Gln 
            20                  25                  30          


Leu Glu Lys Glu Asn Lys Met Ile Asp Met Tyr Glu Thr Val Glu Glu 
        35                  40                  45              


Asn Ile Asn Thr Ile Lys Thr Glu Asn Thr Asn Asp Ile Asn Glu Glu 
    50                  55                  60                  


Val Arg Asn Glu Gln Lys Arg Glu Ser Ile Asn His Ile Asn Asp Thr 
65                  70                  75                  80  


Asn Ile Asn His Ile Ile Asp Glu Tyr Pro Asn Asp Thr Tyr Asn Phe 
                85                  90                  95      


Ile Lys Asp Ile Glu Cys Val His Asn Asn Glu Asn Asn Met Tyr Asn 
            100                 105                 110         


Ser Ile Glu Gln Tyr Thr Phe Tyr His Asp Thr Arg Asn Asn His Leu 
        115                 120                 125             


Val Asp Lys Asn Asn Gln Asn Phe Ile Phe Glu Glu Glu Gly Leu Asn 
    130                 135                 140                 


Glu Leu Asn Phe Glu Glu Lys Lys Val Tyr Ile Glu Asn Asn Thr Lys 
145                 150                 155                 160 


Asp Asp His Lys Gly Asp Ser Lys Thr Ser Asn Leu Thr Ser Leu Arg 
                165                 170                 175     


Asn Thr Ile Cys Lys Ser Glu Asn Asp His Asn Glu Lys Asn Glu Asn 
            180                 185                 190         


Thr Tyr Val Val Arg Lys Gly Glu Lys Gly Ile Lys Arg Lys Val Ser 
        195                 200                 205             


Met Lys Lys Arg Asn Glu Lys Leu Asn Glu Glu Asn Tyr Ile Asn Asn 
    210                 215                 220                 


Ile Tyr Asp Lys Met Asp Asn His Arg Gln Asn Asp Ile 
225                 230                 235         


<210>  4
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Frag2 nucleotide fragment

<400>  4
aatcgtgtta cgaaaaataa atatagtgat atgaataatg ttgaggtatt attaaatgaa       60

gataatttat taactactga aaaatacaag gtgcaattag aaaaagaaaa taaaatgatt      120

gatatgtatg aaacggtaga ggagaatata aatacaatta aaacagaaaa tacgaacgac      180

ataaatgaag aagttagaaa cgaacaaaaa agagaaagta tcaatcatat taatgataca      240

aatataaatc atataataga tgaatatccc aatgatacat ataatttcat aaaagatata      300

gaatgtgtac ataacaatga aaataacatg tacaattcta ttgaacaata tacattttat      360

catgatacac gtaataatca tttagttgat aaaaataatc aaaattttat attcgaagag      420

gaaggtttaa atgaattgaa ctttgaagaa aaaaaggtat atatagaaaa taataccaag      480

gatgatcaca agggagatag caaaacaagt aacttaacat ctttaaggaa taccatatgt      540

aaaagtgaaa acgatcataa tgaaaaaaat gaaaacacat atgtggttag aaaaggcgaa      600

aaaggaatta aacgtaaggt ttccatgaag aaaagaaatg aaaagctaaa tgaagaaaat      660

tatattaata atatatacga taaaatggat aaccatagac aaaatgatat t               711


<210>  5
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfEMMA fragment

<400>  5

Leu Lys Ile Asn Lys Lys Val Pro Lys Tyr Lys Leu Glu Asn Met Asn 
1               5                   10                  15      


Ile Asn Ser Pro Asn Ile Pro Tyr Thr Tyr Leu Phe Ile Cys Asp Gly 
            20                  25                  30          


Ser Asn Tyr Leu Cys Ile Asn Asp Asn Ser Leu Asn Asn Glu Val Tyr 
        35                  40                  45              


Glu Asn Lys Met Lys Leu Asn Asn Ile Ile Gly Tyr Tyr His Tyr Ile 
    50                  55                  60                  


Asn Leu Asn Arg Leu Thr Tyr Tyr Leu Glu Lys Val Asn Ala Asn Phe 
65                  70                  75                  80  


Val Tyr Asn His 
                


<210>  6
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  C-Term nucleotide

<400>  6
ttaaagataa ataagaaagt tcctaaatat aaattggaaa atatgaatat taactcacca       60

aatattccat atacatatct ttttatatgt gatggaagta actatttatg tattaatgac      120

aattcattaa ataacgaggt atatgaaaac aagatgaaat tgaacaatat cattggatat      180

taccattata ttaatttgaa tagattaaca tattatttag aaaaggtaaa tgctaatttt      240

gtttataacc at                                                          252


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of Frag1

<400>  7

Asp Asn Val Asn Asn Asn Asn Asn Lys Glu Ser Cys Asp Asn Ile Lys 
1               5                   10                  15      


His Met Arg Thr Lys Ser Leu Asn Phe Val Ser Arg Glu Ser Tyr Gly 
            20                  25                  30          


Glu His Lys Ser Leu Asp Val Tyr Gln Glu 
        35                  40          


<210>  8
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of Frag1

<400>  8

Cys Tyr Val Lys Asn Asn Lys Leu Ile Asn Lys Val Asn Asp Lys Lys 
1               5                   10                  15      


Tyr Glu Asp Asn Asn Asn Ser Tyr Leu Asn Glu Asp Asp Asn Ala Ser 
            20                  25                  30          


Met Gln Phe Tyr Glu Glu Thr Asn Ser 
        35                  40      


<210>  9
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag1

<400>  9

Asn Pro Tyr Ile Val Asp Gln Glu Asn Asn Met Lys Asn Tyr Val Asn 
1               5                   10                  15      


Asn Val Leu Tyr Asn Asn Asn Ser Asn Tyr Tyr Val Asp Ser Lys Asn 
            20                  25                  30          


Tyr Asp Lys Ser Lys Glu Asn Ala Glu 
        35                  40      


<210>  10
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag1

<400>  10

Asn Lys Ser Asp Asp Ile Leu Asn Asn Glu Asn Ile His Thr Leu Lys 
1               5                   10                  15      


Asp Gln Lys Lys Lys Ile Gln Asn Asn Asn Glu Phe Ile Ser Glu Gln 
            20                  25                  30          


Ala Asp Ile Glu Asn Ile Arg Asn Ser Gln Glu Glu 
        35                  40                  


<210>  11
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag1

<400>  11

Val Tyr Glu Lys Glu His Glu Pro Leu Trp Val Ile Asn Ala Ser Asn 
1               5                   10                  15      


Glu Glu Lys Lys Ser Tyr Glu Glu Leu Ile Tyr Ser Asp Met Ser Ser 
            20                  25                  30          


Asn Arg Val Thr Lys Asn Lys Tyr Ser Asp 
        35                  40          


<210>  12
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag1

<400>  12

Met Asn Asn Val Glu Val Leu Leu Asn Glu Asp Asn Leu Leu Thr Thr 
1               5                   10                  15      


Glu Lys Tyr Lys Val Gln Leu Glu Lys Glu Asn Lys 
            20                  25              


<210>  13
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  13

Asn Arg Val Thr Lys Asn Lys Tyr Ser Asp Met Asn Asn Val Glu Val 
1               5                   10                  15      


Leu Leu Asn Glu Asp Asn Leu Leu Thr Thr Glu Lys Tyr Lys Val Gln 
            20                  25                  30          


Leu Glu Lys Glu Asn Lys Met Ile Asp 
        35                  40      


<210>  14
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  14

Met Tyr Glu Thr Val Glu Glu Asn Ile Asn Thr Ile Lys Thr Glu Asn 
1               5                   10                  15      


Thr Asn Asp Ile Asn Glu Glu Val Arg Asn Glu Gln Lys Arg Glu Ser 
            20                  25                  30          


Ile Asn His Ile Asn Asp Thr Asn Ile Asn His Ile Ile 
        35                  40                  45  


<210>  15
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  15

Asp Glu Tyr Pro Asn Asp Thr Tyr Asn Phe Ile Lys Asp Ile Glu Cys 
1               5                   10                  15      


Val His Asn Asn Glu Asn Asn Met Tyr Asn Ser Ile Glu Gln Tyr Thr 
            20                  25                  30          


Phe Tyr His Asp Thr Arg Asn Asn His Leu 
        35                  40          


<210>  16
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  16

Val Asp Lys Asn Asn Gln Asn Phe Ile Phe Glu Glu Glu Gly Leu Asn 
1               5                   10                  15      


Glu Leu Asn Phe Glu Glu Lys Lys Val Tyr Ile Glu Asn Asn Thr Lys 
            20                  25                  30          


Asp Asp His Lys Gly Asp Ser Lys Thr Ser Asn 
        35                  40              


<210>  17
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  17

Leu Thr Ser Leu Arg Asn Thr Ile Cys Lys Ser Glu Asn Asp His Asn 
1               5                   10                  15      


Glu Lys Asn Glu Asn Thr Tyr Val Val Arg Lys Gly Glu Lys Gly Ile 
            20                  25                  30          


Lys Arg Lys Val Ser Met Lys Lys Arg Asn 
        35                  40          


<210>  18
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of frag2

<400>  18

Glu Lys Leu Asn Glu Glu Asn Tyr Ile Asn Asn Ile Tyr Asp Lys Met 
1               5                   10                  15      


Asp Asn His Arg Gln Asn Asp Ile 
            20                  


<210>  19
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of c-term

<400>  19

Leu Lys Ile Asn Lys Lys Val Pro Lys Tyr Lys Leu Glu Asn Met Asn 
1               5                   10                  15      


Ile Asn Ser Pro Asn Ile Pro Tyr Thr Tyr Leu Phe Ile Cys Asp Gly 
            20                  25                  30          


Ser Asn Tyr Leu Cys Ile Asn Asp Asn Ser Leu Asn 
        35                  40                  


<210>  20
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  antigenic fragment of C-term

<400>  20

Asn Glu Val Tyr Glu Asn Lys Met Lys Leu Asn Asn Ile Ile Gly Tyr 
1               5                   10                  15      


Tyr His Tyr Ile Asn Leu Asn Arg Leu Thr Tyr Tyr Leu Glu Lys Val 
            20                  25                  30          


Asn Ala Asn Phe Val Tyr Asn His 
        35                  40  


<210>  21
<211>  2227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PfEMMA1

<400>  21

Met Arg Ser Lys Ser Ile Ser Tyr Phe Leu Phe Phe Lys Lys Asn Lys 
1               5                   10                  15      


Lys Lys Asn Asp Ser Cys Asp Ser Val Ile Ile Ser Ser Asn Lys Asn 
            20                  25                  30          


Leu Ser Ile Gln Leu Ser Lys Gly Glu Asp Asp Glu Lys Asn Glu Ile 
        35                  40                  45              


Asn Glu Glu Lys Ser Tyr Ile Lys Asn Glu Asp Val Tyr Lys Lys Glu 
    50                  55                  60                  


Lys Leu Lys Lys Lys Lys Glu Asn Lys Glu Asn Asn Lys Lys Lys Asp 
65                  70                  75                  80  


Lys Asn Glu Val Val Tyr Asp Tyr His Asp Ile Ser Asn Asp Ala Thr 
                85                  90                  95      


Ser Asp Tyr Val Asn Asn Tyr Lys Val Tyr Glu Met Asn Thr Cys Asn 
            100                 105                 110         


Ile Lys Lys Lys Arg Glu Ser Phe Phe Lys Lys Ile Asn Ile Leu Gln 
        115                 120                 125             


Lys Tyr Lys Asn Tyr Lys Ile Arg Lys Ala Ala Ser Thr Phe His Thr 
    130                 135                 140                 


Ile Gly His Lys Thr Ser Phe Ser Gly Thr Asp Asp Glu Ile Glu Asn 
145                 150                 155                 160 


Asn Gln Lys Lys Gln Lys Lys Tyr Lys Ile Lys Ile Ser Glu Trp Lys 
                165                 170                 175     


Asp Asp Lys Ser His Thr Phe His Lys Lys Asn Asp Ile Leu Val Phe 
            180                 185                 190         


Asp Lys Met Asp Lys Asn Lys Lys Phe Lys Ile Asp Asn Asn Lys Asn 
        195                 200                 205             


Asn Gln Ile Asn Ile Asp Asn Glu Glu Arg Val Asn Lys Asn Tyr Pro 
    210                 215                 220                 


Met Ala Thr Asn Val Gln Asn Phe Asn Ile Lys Tyr Thr Ser Ile Asp 
225                 230                 235                 240 


Val Thr Asn Asp Glu Tyr Ile Ile Asp Ser Asn Lys Pro Glu Gly Ser 
                245                 250                 255     


Ile Met Ser Thr Asp Lys Lys Asn Asn Lys Leu Asn Tyr Asn Asn Asp 
            260                 265                 270         


Thr Tyr Asp Val Asp Lys Ser Ser Asp Ile Asn Lys Leu Gly Asn Ile 
        275                 280                 285             


Lys Lys Asn Lys Phe Asp Ile Ile Thr Lys Thr Thr His Asn Ile Asn 
    290                 295                 300                 


Asn Asn Val Asn Asn Ile His Asn Tyr Met Met Tyr Thr Asn Lys Glu 
305                 310                 315                 320 


Asn Ile Lys Ile Asn Ile Asn His Gly Asn Leu Asn Gly Arg Glu Gln 
                325                 330                 335     


Asn Asn Tyr Asp Glu Glu Arg Lys Ala Asn Val Tyr Glu Ile Phe Glu 
            340                 345                 350         


Asn Ala Lys Lys Leu Glu Pro Asn Asn Ile Asn Ile Asn Thr Glu Glu 
        355                 360                 365             


His Ile His Ile Ser Glu Pro Ser Ile Pro Phe Asp Met Lys Asp His 
    370                 375                 380                 


Lys Asn Asp Ile Asn Glu Lys Asp Ile Ile Leu Lys Leu Met Tyr Asn 
385                 390                 395                 400 


Asn Asn Gly Ile Tyr Phe Asp Asp Asp Asp Glu Asn His Lys Asn Leu 
                405                 410                 415     


Leu Tyr Lys Asn Lys Asp Thr His Val Lys His Leu Asn Asn Lys Phe 
            420                 425                 430         


Asn His Asn Phe Ile Ile Tyr Asn Asp Arg Glu Glu Gly Val Asn Gln 
        435                 440                 445             


Lys His Ala Gln Lys Lys Leu Lys Lys Lys Asn Thr Ile Leu Asn Lys 
    450                 455                 460                 


Asn Glu Asn Glu Asp Ile Asn His Asn Ser Phe Lys Arg Pro Leu Ser 
465                 470                 475                 480 


Asn Thr Asn Ile Cys Tyr Lys Asp Lys Asp Asp Lys Ile Lys Asn Gly 
                485                 490                 495     


Ser Asn Lys Tyr Asp Ile Leu Asn Asn Asp Tyr Ser Asn Glu His Glu 
            500                 505                 510         


Lys Asn Lys Tyr Asn Asp His Ile Thr Lys Asn Lys Arg Asn Gln Ser 
        515                 520                 525             


Ala Asn Glu Val Lys Ser Asn Asn Asn Asp Asn His Asn Asn Lys Lys 
    530                 535                 540                 


Asn Asn Asn Phe Asn Ile Asn Ile Asn Asp Ser Tyr Ser Thr Asn Ile 
545                 550                 555                 560 


Asn Arg Asn Gln Asn Val Met Ile Asn Asp Val Asn Asp Val Ile Lys 
                565                 570                 575     


Asp Pro Asn Met Gln Glu Asn Thr Gln Gly Asp Asp Glu Gly Gly Ile 
            580                 585                 590         


Ile Asn Lys Tyr Leu Ile Asn Pro Ile Tyr Asn Leu Phe Leu Arg Ala 
        595                 600                 605             


Asn Glu Glu Ile Gln Asn Ser Asn Ser Thr Asn Asn Lys Leu Lys Met 
    610                 615                 620                 


Asn Asn Ile Thr Lys Ser Tyr Thr Asn Glu Leu Gln Lys Thr Tyr Lys 
625                 630                 635                 640 


Ser Met Tyr Asp Ile Asn Asp Ile Ser Asn Lys Arg Lys Ile Asn Asn 
                645                 650                 655     


Lys Asp Ile Arg Gly Thr Asn Leu Tyr Asn Thr Lys Leu Cys Asn Asn 
            660                 665                 670         


Lys Leu Tyr Asn Ser Asn Pro Tyr Asn Met Ile Pro Tyr Asn Ile Asn 
        675                 680                 685             


Thr Tyr Asn Asn Asn Asn Asn Asn Lys Glu Thr Cys Thr Ser Ile Asn 
    690                 695                 700                 


Ile Lys His Ser Glu Asn Lys Tyr Pro Phe Asn Lys Ser His Val Asn 
705                 710                 715                 720 


Ser Tyr Met Lys Asn Thr Asn His Leu Pro His Arg Asn Ala Ile Thr 
                725                 730                 735     


Ser Asn Asn Arg Asn Asn Glu Glu Tyr Glu Lys Glu Lys Glu Lys Asp 
            740                 745                 750         


Arg Asn Ile Thr Asn Gly Asn Asn Asn Tyr Leu Val Glu Tyr Asn Asn 
        755                 760                 765             


Ser Cys Ile Pro Pro Pro Leu Lys Lys Met Ile Pro Ile Asp Gly Val 
    770                 775                 780                 


Arg Asn Lys Ser Ile Asn Lys Leu Asn Asn Val Thr Asn Thr Gln Arg 
785                 790                 795                 800 


Thr Ser Ser Val Ser Tyr Thr Asn Lys Asn Ile Asp Glu Asn Ser Phe 
                805                 810                 815     


Asp Met Pro Ile Ile Asn Gly Ile Arg Glu Ser Lys Tyr Ile Ser Asn 
            820                 825                 830         


Asn Asn Asn Ile Asn Gly Asn Ser Ile Gly Phe Asn Ser Ser Lys Leu 
        835                 840                 845             


Asp Asn Tyr His His Gln Ser Met Asn Val Asn Glu Ser Tyr Pro Leu 
    850                 855                 860                 


Lys Asn Met Met Lys Asn Asn Tyr Ile Glu His Asn Tyr Asp Asp Lys 
865                 870                 875                 880 


Asn Asn Ile Phe Leu Val Lys Asn Tyr Glu Asp Thr Tyr Ser Asn Ile 
                885                 890                 895     


His Asn Gly Ile His Glu Asn Ser Met Leu Lys Asn Tyr Asn Leu Lys 
            900                 905                 910         


Lys Ala Cys Thr Phe His Gly Tyr Ser Arg Asn His Gln Lys Asn Met 
        915                 920                 925             


Tyr Thr Glu Glu Asn Leu Asn Ile Asn Gln Lys Lys Asn Tyr Ser His 
    930                 935                 940                 


Tyr His Asn Asn Gly Thr Val Leu Lys Pro Leu Val Asn Thr Asn Asn 
945                 950                 955                 960 


Val Ala Val Asn Glu Phe Ala Asp Ile Asn Leu Ser Ala Gln Lys Arg 
                965                 970                 975     


Leu His Ser Leu Lys Ser Met Gly Tyr Glu Asp Lys Ser Met Glu Asn 
            980                 985                 990         


Tyr Arg Asn Lys Ile Tyr Asn Asn  Ile Asn Asn Asn Asn  Asn Asn Asn 
        995                 1000                 1005             


Asn Asp  Asn Asn Ile Tyr Asn  Asp Asn Glu Tyr Cys  Gln Tyr Asn 
    1010                 1015                 1020             


Asn Ser  Tyr Cys Phe Asp His  Ser Asp Leu Lys Asn  Met Phe Pro 
    1025                 1030                 1035             


Leu Asn  His Gln Asn Ser Lys  Leu Leu Thr His Ser  Asn Asn Lys 
    1040                 1045                 1050             


Asn Ser  Phe Phe Asn Gly Ile  Asn Val Glu Ser Lys  His His Leu 
    1055                 1060                 1065             


Ala Asn  Pro Glu Ile Lys Thr  Phe Ala His Asn Ser  Tyr Pro Ile 
    1070                 1075                 1080             


Leu Asn  Gln Gly Leu Ile Asn  Cys Asn Pro Leu Gln  Cys Leu Gly 
    1085                 1090                 1095             


Tyr Asp  Ser Asn Gln Arg Asn  Lys His Asn Val Val  Tyr Ile Lys 
    1100                 1105                 1110             


Lys Asn  Glu Tyr Leu Asn Lys  Asn Ile Gly Ser Ile  Ile Asn Val 
    1115                 1120                 1125             


Leu Lys  Arg Glu Gly Leu Arg  Lys Ile Ser Thr His  Asn Gly Lys 
    1130                 1135                 1140             


Phe Glu  Ser Phe Ser Asn Met  Asp Asn Lys Asn Val  Tyr Met Glu 
    1145                 1150                 1155             


Gly Leu  Asn Ile Gln Asp Asn  Val Asn Asn Asn Asn  Asn Lys Glu 
    1160                 1165                 1170             


Ser Cys  Asp Asn Ile Lys His  Met Arg Thr Lys Ser  Leu Asn Phe 
    1175                 1180                 1185             


Val Ser  Arg Glu Ser Tyr Gly  Glu His Lys Ser Leu  Asp Val Tyr 
    1190                 1195                 1200             


Gln Glu  Cys Tyr Val Lys Asn  Asn Lys Leu Ile Asn  Lys Val Asn 
    1205                 1210                 1215             


Asp Lys  Lys Tyr Glu Asp Asn  Asn Asn Ser Tyr Leu  Asn Glu Asp 
    1220                 1225                 1230             


Asp Asn  Ala Ser Met Gln Phe  Tyr Glu Glu Thr Asn  Ser Asn Pro 
    1235                 1240                 1245             


Tyr Ile  Val Asp Gln Glu Asn  Asn Met Lys Asn Tyr  Val Asn Asn 
    1250                 1255                 1260             


Val Leu  Tyr Asn Asn Asn Ser  Asn Tyr Tyr Val Asp  Ser Lys Asn 
    1265                 1270                 1275             


Tyr Asp  Lys Ser Lys Glu Asn  Ala Glu Asn Lys Ser  Asp Asp Ile 
    1280                 1285                 1290             


Leu Asn  Asn Glu Asn Ile His  Thr Leu Lys Asp Gln  Lys Lys Lys 
    1295                 1300                 1305             


Ile Gln  Asn Asn Asn Glu Phe  Ile Ser Glu Gln Ala  Asp Ile Glu 
    1310                 1315                 1320             


Asn Ile  Arg Asn Ser Gln Glu  Glu Val Tyr Glu Lys  Glu His Glu 
    1325                 1330                 1335             


Pro Leu  Trp Val Ile Asn Ala  Ser Asn Glu Glu Lys  Lys Ser Tyr 
    1340                 1345                 1350             


Glu Glu  Leu Ile Tyr Ser Asp  Met Ser Ser Asn Arg  Val Thr Lys 
    1355                 1360                 1365             


Asn Lys  Tyr Ser Asp Met Asn  Asn Val Glu Val Leu  Leu Asn Glu 
    1370                 1375                 1380             


Asp Asn  Leu Leu Thr Thr Glu  Lys Tyr Lys Val Gln  Leu Glu Lys 
    1385                 1390                 1395             


Glu Asn  Lys Met Ile Asp Met  Tyr Glu Thr Val Glu  Glu Asn Ile 
    1400                 1405                 1410             


Asn Thr  Ile Lys Thr Glu Asn  Thr Asn Asp Ile Asn  Glu Glu Val 
    1415                 1420                 1425             


Arg Asn  Glu Gln Lys Arg Glu  Ser Ile Asn His Ile  Asn Asp Thr 
    1430                 1435                 1440             


Asn Ile  Asn His Ile Ile Asp  Glu Tyr Pro Asn Asp  Thr Tyr Asn 
    1445                 1450                 1455             


Phe Ile  Lys Asp Ile Glu Cys  Val His Asn Asn Glu  Asn Asn Met 
    1460                 1465                 1470             


Tyr Asn  Ser Ile Glu Gln Tyr  Thr Phe Tyr His Asp  Thr Arg Asn 
    1475                 1480                 1485             


Asn His  Leu Val Asp Lys Asn  Asn Gln Asn Phe Ile  Phe Glu Glu 
    1490                 1495                 1500             


Glu Gly  Leu Asn Glu Leu Asn  Phe Glu Glu Lys Lys  Val Tyr Ile 
    1505                 1510                 1515             


Glu Asn  Asn Thr Lys Asp Asp  His Lys Gly Asp Ser  Lys Thr Ser 
    1520                 1525                 1530             


Asn Leu  Thr Ser Leu Arg Asn  Thr Ile Cys Lys Ser  Glu Asn Asp 
    1535                 1540                 1545             


His Asn  Glu Lys Asn Glu Asn  Thr Tyr Val Val Arg  Lys Gly Glu 
    1550                 1555                 1560             


Lys Gly  Ile Lys Arg Lys Val  Ser Met Lys Lys Arg  Asn Glu Lys 
    1565                 1570                 1575             


Leu Asn  Glu Glu Asn Tyr Ile  Asn Asn Ile Tyr Asp  Lys Met Asp 
    1580                 1585                 1590             


Asn His  Arg Gln Asn Asp Ile  Thr Lys Lys Glu Asn  Asp Glu Glu 
    1595                 1600                 1605             


Asn Tyr  Ile Leu Tyr Asn Asn  Val Lys Val Asn Tyr  Asp Glu Tyr 
    1610                 1615                 1620             


Ile Glu  Asn Gly Asn Lys Ile  Lys Ile Thr Glu Glu  Ser Leu Asn 
    1625                 1630                 1635             


Val Phe  Tyr Lys Glu Asn Gln  Asn Glu Glu Asp Ser  Ser Thr Lys 
    1640                 1645                 1650             


Lys Leu  Asn Ser Thr Ser Lys  Ile Lys Arg Ala Asn  Lys Gly Lys 
    1655                 1660                 1665             


Thr Lys  Lys Lys Asn Val Ile  Thr Arg Val His Lys  Thr Lys Gln 
    1670                 1675                 1680             


Lys Ile  Glu Tyr Val Thr Asn  Ser Phe Asn Lys Ser  Ser Lys Gly 
    1685                 1690                 1695             


Glu Asn  Ser Glu Ile Gly Lys  Ile Gly Gly Arg Ser  Lys Ser Leu 
    1700                 1705                 1710             


Leu Thr  His Ser Lys Lys Val  Ser Glu Arg Asn Lys  Asn Lys Ile 
    1715                 1720                 1725             


Glu Lys  Ile Asn Asp Thr Asn  Ser Lys Ile Ile Lys  Gly Lys Lys 
    1730                 1735                 1740             


Ser Asn  Ser Gln Ser Lys Leu  Gly Lys Asp Thr Lys  Ile Arg Gly 
    1745                 1750                 1755             


Lys Ser  Lys Thr Gly Glu Tyr  Ile Lys Asn Lys Asp  Leu Arg Lys 
    1760                 1765                 1770             


Lys Ser  Asn Glu Lys Asn Lys  Thr Val Met Asp Asn  Ile Asn Thr 
    1775                 1780                 1785             


Ile Asn  Asn Ser Ser Val Ser  Asn Leu Lys Ser Lys  Lys His Lys 
    1790                 1795                 1800             


Leu Lys  Lys Lys Lys Lys Lys  Asn Ile Ser Met Glu  Asn Ile Asn 
    1805                 1810                 1815             


Lys Asn  Ile Thr Asn Glu Phe  Cys Ser Met Glu Arg  Lys Gly Thr 
    1820                 1825                 1830             


Val Leu  Leu Ser Asn Met Ser  Ile Lys Lys Ile Asp  Asn Ala Asn 
    1835                 1840                 1845             


Ser Cys  Thr Leu Asn Glu Pro  Leu Glu Glu Asn Thr  Leu Asn Tyr 
    1850                 1855                 1860             


Glu Ser  Asn Asn Asn Cys Ser  Asn Ser Asn Leu Ser  Lys Asp Lys 
    1865                 1870                 1875             


Glu Lys  Asp Arg Asn Ile Leu  Cys Asn Lys Tyr Tyr  Ser Asp Glu 
    1880                 1885                 1890             


Glu Thr  Asn Ser Leu Asn Lys  Met Tyr Thr Ser Asn  Ile Pro Glu 
    1895                 1900                 1905             


Ile Ser  Asn Tyr Tyr Lys Glu  Ile Gln Ala Ile Asn  Tyr Ile Leu 
    1910                 1915                 1920             


Ser Asn  Ile Asn Asn Pro Asn  Phe Leu Asn Ser Leu  Glu Leu Asn 
    1925                 1930                 1935             


Asp Leu  Ile Asn Ile Glu Lys  Lys Phe Ile Asn Glu  Asn Ile Tyr 
    1940                 1945                 1950             


Ile Asn  Lys Gln Ile Ile Ala  Cys Asn Val Lys Asn  Glu Lys Ser 
    1955                 1960                 1965             


Asn Asp  Glu Met Val Glu Lys  Asn Glu Arg Lys Val  Asp Glu Glu 
    1970                 1975                 1980             


Lys Gly  Glu Asp Glu Gln Glu  Ile Lys Ala Lys Glu  Asn Asn Asn 
    1985                 1990                 1995             


Lys Glu  Glu Asn Gln Asp Asn  Glu Asn Asn Asn Lys  Glu Glu Asn 
    2000                 2005                 2010             


His Asp  Asn Glu Asn Asn Asn  Lys Glu Glu Asn Gln  Asp Asn Glu 
    2015                 2020                 2025             


Asn Asn  Asn Lys Glu Glu Asn  Gln Asp Asn Glu Asn  Asn Asn Lys 
    2030                 2035                 2040             


Glu Glu  Asn Gln Asp Asn Glu  Asn Asn Asn Lys Glu  Glu Asn Gln 
    2045                 2050                 2055             


Lys Asn  Glu Asn Gly Ile Ile  Tyr Asp Ser Arg Phe  Ser Ile Ile 
    2060                 2065                 2070             


Tyr Leu  Glu His Asp Leu Ile  Tyr Leu Lys Lys Asn  Asn Leu Lys 
    2075                 2080                 2085             


Val Ile  Leu Asn Val Leu Leu  Ser Asn Val Tyr Cys  Phe Phe Glu 
    2090                 2095                 2100             


Ile Lys  Leu Thr Ile Ile Leu  Leu Asn Phe Phe Ile  Ser Asn Asn 
    2105                 2110                 2115             


Cys Gln  Trp Ser Phe Ser Leu  Phe Pro Leu Ser Leu  Ile Asn Lys 
    2120                 2125                 2130             


Leu Ile  His Lys Phe Ser Leu  Lys Ile Asn Lys Lys  Val Pro Lys 
    2135                 2140                 2145             


Tyr Lys  Leu Glu Asn Met Asn  Ile Asn Ser Pro Asn  Ile Pro Tyr 
    2150                 2155                 2160             


Thr Tyr  Leu Phe Ile Cys Asp  Gly Ser Asn Tyr Leu  Cys Ile Asn 
    2165                 2170                 2175             


Asp Asn  Ser Leu Asn Asn Glu  Val Tyr Glu Asn Lys  Met Lys Leu 
    2180                 2185                 2190             


Asn Asn  Ile Ile Gly Tyr Tyr  His Tyr Ile Asn Leu  Asn Arg Leu 
    2195                 2200                 2205             


Thr Tyr  Tyr Leu Glu Lys Val  Asn Ala Asn Phe Val  Tyr Asn His 
    2210                 2215                 2220             


His Ile  Tyr Glu 
    2225         


<210>  22
<211>  6684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PfEMMA1 nucleotide

<400>  22
atgagatcga aatccatttc gtatttctta ttttttaaaa aaaacaaaaa gaaaaatgat       60

tcttgtgata gtgtcataat atctagcaat aagaatttat ccattcaatt atcgaaaggt      120

gaggatgatg aaaaaaatga aataaatgag gaaaagagtt atataaaaaa tgaagatgta      180

tataaaaagg aaaaattaaa aaagaagaaa gaaaacaagg aaaataataa aaagaaagat      240

aaaaatgaag tagtatatga ttatcatgac atttcaaatg atgctactag tgattatgtt      300

aataattata aagtatatga aatgaatact tgtaatataa aaaagaagag agaaagtttt      360

tttaaaaaaa ttaatatttt acaaaaatat aaaaattaca aaattagaaa ggcagctagt      420

acctttcata ccataggaca taaaacatct ttttctggta cagatgatga aatagaaaat      480

aatcaaaaga aacaaaaaaa atataaaata aaaatttctg aatggaagga tgataaatca      540

catacttttc ataaaaaaaa tgacatattg gtatttgata agatggataa aaataaaaaa      600

tttaaaattg ataacaacaa aaacaatcaa attaatatag ataatgaaga aagagttaat      660

aaaaattatc ctatggctac taatgtacaa aattttaata taaaatatac atcaatagat      720

gtaacaaatg acgaatatat tatagattct aataaacctg aaggttctat tatgtctaca      780

gataaaaaga ataataaact taattataat aatgatacat atgatgtaga caaaagctct      840

gatataaata agttaggtaa tataaaaaag aataaatttg atattattac taaaacaaca      900

cataatatta ataataatgt aaataatata cataattata tgatgtatac aaataaagaa      960

aatataaaaa taaatataaa tcatggaaat ctaaatggaa gagaacaaaa caattatgat     1020

gaagaaagga aagcaaatgt ttatgaaata tttgaaaatg caaaaaaatt agaacctaat     1080

aatattaata tcaacacaga agaacatatt catattagtg aacccagcat accatttgat     1140

atgaaggatc ataaaaatga tataaatgaa aaagatataa tattaaaatt gatgtataac     1200

aataacggta tttattttga tgatgatgat gaaaatcaca agaatttatt atacaaaaat     1260

aaagatacac atgtaaaaca tttaaataat aaatttaacc ataattttat tatatataat     1320

gatcgcgaag aaggggtaaa tcagaaacac gcacaaaaaa aattaaaaaa aaaaaatact     1380

attcttaaca aaaacgaaaa tgaagatatt aatcataata gtttcaaaag acctttatct     1440

aatacgaata tatgttataa ggacaaagat gataaaatta aaaatggttc taataagtat     1500

gatatattaa ataatgacta ttctaatgaa cacgaaaaaa ataaatataa tgatcatata     1560

acaaaaaata aaagaaatca atcagcaaat gaagtaaaat ctaataataa tgataaccac     1620

aataataaaa aaaataataa ttttaatatt aatattaatg attcatattc tacaaatata     1680

aatagaaacc aaaatgtgat gataaatgat gtaaacgatg ttattaagga tccaaatatg     1740

caggaaaata cacaaggtga tgacgaaggt ggtattataa acaaatattt aattaaccct     1800

atttacaatt tatttctacg tgctaatgaa gaaatacaaa attcaaatag tacaaacaat     1860

aaattaaaaa tgaataatat aacaaaaagt tatacaaacg aactacaaaa gacatataaa     1920

agtatgtacg atataaatga tatatcaaat aagagaaaaa ttaataataa agatatacgt     1980

ggaactaatt tgtataacac caaattatgt aataataaat tatataattc gaatccatat     2040

aatatgattc catataatat aaacacatat aataataata ataataataa ggaaacttgt     2100

accagcataa atatcaaaca ttccgaaaat aaatatccct tcaataaatc tcatgtaaac     2160

tcatatatga aaaatacaaa tcatcttcct catagaaatg cgattacatc aaataataga     2220

aacaatgaag aatatgagaa agaaaaagaa aaagatcgta acattactaa tgggaacaat     2280

aattatttgg ttgaatataa taattcttgt atacctccac cactcaaaaa aatgatacca     2340

atagatggtg tgagaaataa aagtataaat aaattaaata atgtaactaa tacgcaacgt     2400

acatcaagtg tttcatatac gaataagaat attgatgaga attcgtttga tatgcctata     2460

ataaatggaa taagagaatc taaatatata agtaataata ataatattaa tggtaattcc     2520

attggtttta attcatctaa gttagataat tatcatcacc aatctatgaa tgtgaatgaa     2580

tcttatcctc taaaaaatat gatgaaaaat aattatattg aacataatta tgatgataaa     2640

aataatattt tccttgttaa aaattatgaa gatacatatt caaatattca taatggcata     2700

catgaaaata gcatgctaaa aaattataat ttaaaaaaag cgtgcacttt tcatgggtac     2760

tctagaaatc accaaaaaaa tatgtatacg gaagaaaatt taaatattaa tcaaaaaaag     2820

aattatagtc attatcataa taatggaacg gtattaaaac ctttggtaaa tactaataat     2880

gttgcagtga acgaatttgc agatattaat ttatcggctc aaaaaagatt acatagttta     2940

aaaagtatgg ggtacgagga taagagtatg gaaaattaca gaaacaaaat atacaacaac     3000

atcaataata ataataataa taataatgat aataatatat ataatgataa tgaatattgt     3060

cagtataata atagttattg tttcgatcat agtgatttaa aaaatatgtt tccattaaat     3120

catcagaata gcaagttatt aacacatagt aataataaaa attcattttt taacggaata     3180

aatgtagaat cgaaacatca tttagcaaat cctgaaataa aaacatttgc acacaatagt     3240

tatcctatat taaatcaagg tttaataaat tgtaacccct tacaatgctt gggttatgat     3300

tcaaatcaaa ggaataagca taatgtagta tacataaaaa aaaatgaata ccttaataaa     3360

aacattggct ctattataaa tgttcttaaa agagaaggac taagaaaaat ttctacacat     3420

aatggaaaat tcgaatcatt tagtaatatg gataataaaa atgtatatat ggaaggacta     3480

aacatacaag ataatgttaa taataataat aataaagaaa gttgtgataa tattaaacat     3540

atgagaacaa aaagtttaaa ttttgtaagt agagaatcct atggcgaaca taaaagtcta     3600

gatgtttacc aggaatgtta tgtaaaaaat aataaactta ttaataaggt aaatgataaa     3660

aaatatgagg acaataataa ttcctatctt aatgaagatg ataacgctag tatgcaattt     3720

tatgaagaaa ctaatagtaa tccatatatt gtagaccagg aaaataatat gaaaaattat     3780

gtcaataatg ttttatataa caacaatagc aattattatg ttgattcaaa gaattatgat     3840

aaatctaaag agaatgcaga aaataaatca gatgatatat taaataatga aaatatacat     3900

accttaaaag atcaaaaaaa gaaaatacaa aataataatg aattcattag tgaacaggct     3960

gatatagaaa atataagaaa ttctcaagaa gaagtatatg agaaagaaca cgaacctttg     4020

tgggtaataa atgcatctaa tgaagaaaag aaatcatatg aagaattgat atacagcgat     4080

atgtcatcta atcgtgttac gaaaaataaa tatagtgata tgaataatgt tgaggtatta     4140

ttaaatgaag ataatttatt aactactgaa aaatacaagg tgcaattaga aaaagaaaat     4200

aaaatgattg atatgtatga aacggtagag gagaatataa atacaattaa aacagaaaat     4260

acgaacgaca taaatgaaga agttagaaac gaacaaaaaa gagaaagtat caatcatatt     4320

aatgatacaa atataaatca tataatagat gaatatccca atgatacata taatttcata     4380

aaagatatag aatgtgtaca taacaatgaa aataacatgt acaattctat tgaacaatat     4440

acattttatc atgatacacg taataatcat ttagttgata aaaataatca aaattttata     4500

ttcgaagagg aaggtttaaa tgaattgaac tttgaagaaa aaaaggtata tatagaaaat     4560

aataccaagg atgatcacaa gggagatagc aaaacaagta acttaacatc tttaaggaat     4620

accatatgta aaagtgaaaa cgatcataat gaaaaaaatg aaaacacata tgtggttaga     4680

aaaggcgaaa aaggaattaa acgtaaggtt tccatgaaga aaagaaatga aaagctaaat     4740

gaagaaaatt atattaataa tatatacgat aaaatggata accatagaca aaatgatatt     4800

acaaaaaaag aaaatgacga agaaaattat attttgtaca acaacgtaaa ggttaattat     4860

gatgaatata tagaaaatgg aaataaaata aaaataacgg aagaatcatt aaatgtcttt     4920

tataaagaaa atcaaaatga ggaagattct tctacaaaaa agttgaatag tacaagtaaa     4980

ataaaacgtg caaacaaagg gaaaacaaaa aaaaagaatg ttatcacaag ggtacataaa     5040

acaaaacaaa aaattgaata tgttacaaat agttttaata aatcttccaa aggtgaaaat     5100

tcagaaatag gaaaaattgg aggtaggagt aaatcattat taacacacag caagaaagtt     5160

agtgaacgaa ataaaaataa aatagaaaaa attaatgata caaattcaaa gataataaaa     5220

ggaaaaaaga gtaatagcca aagcaaactt gggaaggata caaaaattag agggaaatca     5280

aaaactgggg aatatataaa aaataaagat ttaagaaaaa aatctaacga aaaaaacaaa     5340

acagtgatgg ataatataaa tactataaat aattcttcag tatctaacct aaaaagcaaa     5400

aaacataaat tgaaaaaaaa aaaaaaaaaa aatatatcta tggaaaatat aaataaaaat     5460

ataacaaatg aattttgttc tatggaaaga aaaggaaccg ttctattatc taatatgagt     5520

attaagaaga ttgataatgc aaatagttgt acattaaatg aaccattaga ggaaaatacc     5580

ttaaattatg aaagtaataa taactgtagt aatagtaatt tatctaagga taaagaaaaa     5640

gatagaaata tattgtgtaa taaatattat agtgatgagg aaacaaactc tttaaacaaa     5700

atgtatacat cgaatatacc agaaataagt aattattata aggaaattca agcaattaat     5760

tacatattaa gtaatattaa taatccaaat tttttaaatt ccctcgaact gaatgattta     5820

ataaatattg aaaaaaaatt tattaacgaa aatatatata ttaataagca gataatagcc     5880

tgtaatgtaa aaaatgaaaa atcaaatgat gagatggtcg agaaaaatga acgcaaagtg     5940

gatgaagaaa aaggagaaga cgaacaagaa ataaaagcaa aggaaaataa taataaagaa     6000

gaaaaccaag ataatgaaaa taataataaa gaagaaaacc atgataatga aaataataat     6060

aaagaagaaa atcaagataa tgaaaataat aataaagaag aaaaccaaga taatgaaaat     6120

aataataaag aagaaaatca agataatgaa aataataata aagaagaaaa ccaaaaaaat     6180

gaaaatggta ttatttatga tagcaggttt agtattatct atttagaaca cgatttaata     6240

tatttaaaaa aaaataattt aaaagtgata cttaatgttt tgctgtcaaa tgtgtattgc     6300

ttttttgaaa ttaaattaac cataatattg ttaaatttct ttatatctaa taattgtcaa     6360

tggagtttca gtttatttcc cctttcatta attaataaat taatacataa attcagttta     6420

aagataaata agaaagttcc taaatataaa ttggaaaata tgaatattaa ctcaccaaat     6480

attccatata catatctttt tatatgtgat ggaagtaact atttatgtat taatgacaat     6540

tcattaaata acgaggtata tgaaaacaag atgaaattga acaatatcat tggatattac     6600

cattatatta atttgaatag attaacatat tatttagaaa aggtaaatgc taattttgtt     6660

tataaccatc atatatatga ataa                                            6684


<210>  23
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nterm frag1 of PfEMMA1

<400>  23

Met Arg Ser Lys Ser Ile Ser Tyr Phe Leu Phe Phe Lys Lys Asn Lys 
1               5                   10                  15      


Lys Lys Asn Asp Ser Cys Asp Ser Val Ile Ile Ser Ser Asn Lys Asn 
            20                  25                  30          


Leu Ser Ile Gln Leu Ser Lys Gly Glu Asp Asp Glu Lys Asn Glu Ile 
        35                  40                  45              


Asn Glu Glu Lys Ser Tyr Ile Lys Asn Glu Asp Val Tyr Lys Lys Glu 
    50                  55                  60                  


Lys Leu Lys Lys Lys Lys Glu Asn Lys Glu Asn Asn Lys Lys Lys Asp 
65                  70                  75                  80  


Lys Asn Glu Val Val Tyr Asp Tyr His Asp Ile Ser Asn Asp Ala Thr 
                85                  90                  95      


Ser Asp Tyr Val Asn Asn Tyr Lys Val Tyr Glu Met Asn Thr Cys Asn 
            100                 105                 110         


Ile Lys Lys Lys Arg Glu Ser Phe Phe Lys Lys Ile Asn Ile Leu Gln 
        115                 120                 125             


Lys Tyr Lys Asn Tyr Lys Ile Arg Lys Ala Ala Ser Thr Phe His Thr 
    130                 135                 140                 


Ile Gly His Lys Thr Ser Phe Ser Gly Thr Asp Asp Glu Ile Glu Asn 
145                 150                 155                 160 


Asn Gln Lys Lys Gln Lys Lys Tyr Lys Ile Lys Ile Ser Glu Trp Lys 
                165                 170                 175     


Asp Asp Lys Ser His Thr Phe His Lys Lys Asn Asp Ile Leu Val Phe 
            180                 185                 190         


Asp Lys Met Asp Lys Asn Lys Lys Phe Lys Ile Asp Asn Asn Lys Asn 
        195                 200                 205             


Asn Gln Ile Asn Ile Asp Asn Glu Glu Arg Val Asn Lys Asn Tyr Pro 
    210                 215                 220                 


Met Ala Thr Asn Val Gln Asn Phe Asn Ile Lys Tyr Thr Ser Ile Asp 
225                 230                 235                 240 


Val Thr Asn Asp Glu Tyr Ile Ile Asp Ser Asn Lys Pro Glu Gly Ser 
                245                 250                 255     


Ile Met Ser Thr Asp Lys Lys Asn Asn Lys Leu Asn Tyr Asn Asn Asp 
            260                 265                 270         


Thr Tyr Asp Val Asp 
        275         


<210>  24
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal fragment 2 of PfEMMA1

<400>  24

Lys Ser Ser Asp Ile Asn Lys Leu Gly Asn Ile Lys Lys Asn Lys Phe 
1               5                   10                  15      


Asp Ile Ile Thr Lys Thr Thr His Asn Ile Asn Asn Asn Val Asn Asn 
            20                  25                  30          


Ile His Asn Tyr Met Met Tyr Thr Asn Lys Glu Asn Ile Lys Ile Asn 
        35                  40                  45              


Ile Asn His Gly Asn Leu Asn Gly Arg Glu Gln Asn Asn Tyr Asp Glu 
    50                  55                  60                  


Glu Arg Lys Ala Asn Val Tyr Glu Ile Phe Glu Asn Ala Lys Lys Leu 
65                  70                  75                  80  


Glu Pro Asn Asn Ile Asn Ile Asn Thr Glu Glu His Ile His Ile Ser 
                85                  90                  95      


Glu Pro Ser Ile Pro Phe Asp Met Lys Asp His Lys Asn Asp Ile Asn 
            100                 105                 110         


Glu Lys Asp Ile Ile Leu Lys Leu Met Tyr Asn Asn Asn Gly Ile Tyr 
        115                 120                 125             


Phe Asp Asp Asp Asp Glu Asn His Lys Asn Leu Leu Tyr Lys Asn Lys 
    130                 135                 140                 


Asp Thr His Val Lys His Leu Asn Asn Lys Phe Asn His Asn Phe Ile 
145                 150                 155                 160 


Ile Tyr Asn Asp Arg Glu Glu Gly Val Asn Gln Lys His Ala Gln Lys 
                165                 170                 175     


Lys Leu Lys Lys Lys Asn Thr Ile Leu Asn Lys Asn Glu Asn Glu Asp 
            180                 185                 190         


Ile Asn His Asn Ser Phe Lys Arg Pro Leu Ser Asn Thr Asn Ile Cys 
        195                 200                 205             


Tyr Lys Asp Lys Asp Asp Lys Ile Lys Asn Gly Ser Asn Lys Tyr Asp 
    210                 215                 220                 


Ile Leu Asn Asn Asp Tyr Ser Asn Glu His Glu Lys Asn Lys Tyr Asn 
225                 230                 235                 240 


Asp His Ile Thr Lys Asn Lys Arg Asn Gln Ser Ala Asn Glu Val Lys 
                245                 250                 255     


Ser Asn Asn Asn Asp Asn His Asn Asn Lys Lys Asn Asn Asn Phe Asn 
            260                 265                 270         


Ile Asn Ile Asn Asp 
        275         


<210>  25
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal fragment 3 pf PfEMMA1

<400>  25

Ser Tyr Ser Thr Asn Ile Asn Arg Asn Gln Asn Val Met Ile Asn Asp 
1               5                   10                  15      


Val Asn Asp Val Ile Lys Asp Pro Asn Met Gln Glu Asn Thr Gln Gly 
            20                  25                  30          


Asp Asp Glu Gly Gly Ile Ile Asn Lys Tyr Leu Ile Asn Pro Ile Tyr 
        35                  40                  45              


Asn Leu Phe Leu Arg Ala Asn Glu Glu Ile Gln Asn Ser Asn Ser Thr 
    50                  55                  60                  


Asn Asn Lys Leu Lys Met Asn Asn Ile Thr Lys Ser Tyr Thr Asn Glu 
65                  70                  75                  80  


Leu Gln Lys Thr Tyr Lys Ser Met Tyr Asp Ile Asn Asp Ile Ser Asn 
                85                  90                  95      


Lys Arg Lys Ile Asn Asn Lys Asp Ile Arg Gly Thr Asn Leu Tyr Asn 
            100                 105                 110         


Thr Lys Leu Cys Asn Asn Lys Leu Tyr Asn Ser Asn Pro Tyr Asn Met 
        115                 120                 125             


Ile Pro Tyr Asn Ile Asn Thr Tyr Asn Asn Asn Asn Asn Asn Lys Glu 
    130                 135                 140                 


Thr Cys Thr Ser Ile Asn Ile Lys His Ser Glu Asn Lys Tyr Pro Phe 
145                 150                 155                 160 


Asn Lys Ser His Val Asn Ser Tyr Met Lys Asn Thr Asn His Leu Pro 
                165                 170                 175     


His Arg Asn Ala Ile Thr Ser Asn Asn Arg Asn Asn Glu Glu Tyr Glu 
            180                 185                 190         


Lys Glu Lys Glu Lys Asp Arg Asn Ile Thr Asn Gly Asn Asn Asn Tyr 
        195                 200                 205             


Leu Val Glu Tyr Asn Asn Ser Cys Ile Pro Pro Pro Leu Lys Lys Met 
    210                 215                 220                 


Ile Pro Ile Asp Gly Val Arg Asn Lys Ser Ile Asn Lys Leu Asn Asn 
225                 230                 235                 240 


Val Thr Asn Thr Gln Arg Thr Ser Ser Val Ser Tyr Thr Asn Lys Asn 
                245                 250                 255     


Ile Asp Glu Asn Ser Phe Asp Met Pro Ile Ile Asn Gly Ile Arg Glu 
            260                 265                 270         


Ser Lys Tyr Ile Ser Asn Asn Asn Asn 
        275                 280     


<210>  26
<211>  328
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal fragment 4 of PfEMMA1

<400>  26

Ile Asn Gly Asn Ser Ile Gly Phe Asn Ser Ser Lys Leu Asp Asn Tyr 
1               5                   10                  15      


His His Gln Ser Met Asn Val Asn Glu Ser Tyr Pro Leu Lys Asn Met 
            20                  25                  30          


Met Lys Asn Asn Tyr Ile Glu His Asn Tyr Asp Asp Lys Asn Asn Ile 
        35                  40                  45              


Phe Leu Val Lys Asn Tyr Glu Asp Thr Tyr Ser Asn Ile His Asn Gly 
    50                  55                  60                  


Ile His Glu Asn Ser Met Leu Lys Asn Tyr Asn Leu Lys Lys Ala Cys 
65                  70                  75                  80  


Thr Phe His Gly Tyr Ser Arg Asn His Gln Lys Asn Met Tyr Thr Glu 
                85                  90                  95      


Glu Asn Leu Asn Ile Asn Gln Lys Lys Asn Tyr Ser His Tyr His Asn 
            100                 105                 110         


Asn Gly Thr Val Leu Lys Pro Leu Val Asn Thr Asn Asn Val Ala Val 
        115                 120                 125             


Asn Glu Phe Ala Asp Ile Asn Leu Ser Ala Gln Lys Arg Leu His Ser 
    130                 135                 140                 


Leu Lys Ser Met Gly Tyr Glu Asp Lys Ser Met Glu Asn Tyr Arg Asn 
145                 150                 155                 160 


Lys Ile Tyr Asn Asn Ile Asn Asn Asn Asn Asn Asn Asn Asn Asp Asn 
                165                 170                 175     


Asn Ile Tyr Asn Asp Asn Glu Tyr Cys Gln Tyr Asn Asn Ser Tyr Cys 
            180                 185                 190         


Phe Asp His Ser Asp Leu Lys Asn Met Phe Pro Leu Asn His Gln Asn 
        195                 200                 205             


Ser Lys Leu Leu Thr His Ser Asn Asn Lys Asn Ser Phe Phe Asn Gly 
    210                 215                 220                 


Ile Asn Val Glu Ser Lys His His Leu Ala Asn Pro Glu Ile Lys Thr 
225                 230                 235                 240 


Phe Ala His Asn Ser Tyr Pro Ile Leu Asn Gln Gly Leu Ile Asn Cys 
                245                 250                 255     


Asn Pro Leu Gln Cys Leu Gly Tyr Asp Ser Asn Gln Arg Asn Lys His 
            260                 265                 270         


Asn Val Val Tyr Ile Lys Lys Asn Glu Tyr Leu Asn Lys Asn Ile Gly 
        275                 280                 285             


Ser Ile Ile Asn Val Leu Lys Arg Glu Gly Leu Arg Lys Ile Ser Thr 
    290                 295                 300                 


His Asn Gly Lys Phe Glu Ser Phe Ser Asn Met Asp Asn Lys Asn Val 
305                 310                 315                 320 


Tyr Met Glu Gly Leu Asn Ile Gln 
                325             


<210>  27
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PfEMMA1 Frag 1

<400>  27
gacaatgtca ataacaataa caacaaagag tcctgtgaca acatcaagca catgcgcacg       60

aaaagcctga actttgtgag ccgtgagagc tatggtgagc ataagagcct ggacgtctac      120

caggaatgct acgtgaaaaa caataagctg attaacaaag ttaacgataa gaaatacgaa      180

gataacaaca attcctattt gaacgaagat gacaatgcgt cgatgcagtt ctatgaagag      240

actaacagca atccgtacat cgttgaccaa gagaacaata tgaaaaacta cgtgaataac      300

gtgctgtaca ataacaattc taattactac gttgattcca agaattatga caaaagcaaa      360

gaaaatgccg agaacaagag cgacgacatc ctgaataacg aaaacattca caccctgaaa      420

gatcagaaga aaaagatcca gaacaataac gaattcatta gcgagcaagc ggacatcgag      480

aatatccgta atagccaaga agaggtttat gagaaagaac atgagccgct gtgggtgatt      540

aatgctagca acgaagagaa gaaaagctac gaagaattga tttacagcga tatgagcagc      600

aaccgcgtca cgaagaataa gtatagcgat atgaataacg ttgaagtcct gctgaatgag      660

gacaatctgc tgaccaccga gaagtataaa gttcagctgg agaaagaaaa caag            714


<210>  28
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFEMMA1 Cterm

<400>  28
ctgaagatta acaaaaaagt tccgaagtat aaactggaga acatgaatat caatagcccg       60

aacattccgt atacctactt gtttatctgt gacggttcca attatctgtg catcaacgac      120

aacagcctga ataacgaagt gtatgagaac aagatgaagc tgaacaatat cattggctac      180

taccattaca ttaacctgaa tcgcctgacg tactatctgg aaaaagttaa cgccaatttc      240

gtctacaatc ac                                                          252


<210>  29
<211>  675
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PbEMMA1 frag1

<400>  29
accaatagct atacgaacaa catgctgatt aatgattgta acaatctgca gaacaagagc       60

catcgctata tccgtgcgaa gtccttgaat gcgctgcgcg acagcattct gcacagcaaa      120

tctttaaagg tctacgaaga ttgctgcatc aagaacaaaa atgaaaacat cacgaatcac      180

gactacaata tgaaagacat ctacaagcgt tgcagctaca tcggtaatga aaacacccat      240

gaaatcttct gcaccgagaa atctgagatc tgtgagaacg atctgaacgg ttataacatt      300

aaaccggtgc tgattaagtc ccacgccgat gaaacgaatg aaaagaccaa taacattctg      360

ctgaatgaca tcaacagcca gattgcacac aagaaaaatg acaataacga aaacatttac      420

ttgctggaga aagagaacaa gattgagcaa atcctgacca acctgtccaa taatgaaaac      480

gatagcgaca attgtaccag cgtgacgatt cagcactaca ccgttaacaa tgccgacaac      540

aataaagtcg gcgatcacgt tctgtcgagc tacaacattt gcgagcaaga agagagcctg      600

tggatgccga atcagtataa gatcgagaaa gcgatcctga aagagagcag cgagaactat      660

attcataaag aggaa                                                       675


<210>  30
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PbEMMA1 frag2

<400>  30
ccgaaccagt ataagattga gaaagcaatc ctgaaagaga gctccgagaa ttacattcat       60

aaggaagaat tccagaacgg tctggtgaag tgccgtaaca acttcatcga taatggtatc      120

aaccaatgcg aagatatgct gagcggcgtt attgagaaaa aagaaaatac caattgtaag      180

attaatgacg acgagaaaaa gaatttcaac gataaagttg accagaatga taaagaaatg      240

attgacatct gcgtgaacag ctctagcgag aactgctaca tcaagaataa cagcaacaat      300

cacaacaaca agtatagcat tgaaaaagaa tccaagaatc tgaaagatgt cgcccacgac      360

gactttgagc acaccacgat gaccaccttt acgattatca acccgaacat cattaccagc      420

tctaccttcg agaataatgc atcgacgtgt gagatgatta agaactacaa tccgaataaa      480

gagtgccgca acgtcgagat caataacgac gcgaaaaaag aaaaagaaaa atacgacaat      540

aagaaaaaga tcactaagag caccaataac agcagcaaga tcggcaaaaa caataagctg      600

tgtaagctga aaaaagcgag cattacgaag aaaaactgga agaaccgtga gattggtgag      660

ctgagcgaga atgaaatcta caagctgccg agctatttg                             699


