                         SEQUENCE LISTING

<110>  Crescendo Biologics Limited
 
<120>  Single domain antibodies that bind human serum albumin

<130>  P35198WO1

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  122
<212>  PRT
<213>  homo sapiens

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Ser Cys Ala Ala Ser Gly Phe Thr Phe His His Tyr Ala 
            20                  25                  30          


Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser 
        35                  40                  45              


Gly Ile Ser Trp Asn Gly Asn Lys Ile Thr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys Val 
                85                  90                  95      


Arg Asp Ser Ser Leu Phe Ile Val Gly Ala Pro Thr Phe Glu His Trp 
            100                 105                 110         


Gly Arg Gly Thr Leu Val Thr Val Ser Ser 
        115                 120         


<210>  2
<211>  5
<212>  PRT
<213>  homo sapiens

<400>  2

His Tyr Ala Met His 
1               5   


<210>  3
<211>  17
<212>  PRT
<213>  homo sapiens

<400>  3

Gly Ile Ser Trp Asn Gly Asn Lys Ile Thr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  4
<211>  14
<212>  PRT
<213>  homo sapiens

<400>  4

Asp Ser Ser Leu Phe Ile Val Gly Ala Pro Thr Phe Glu His 
1               5                   10                  


<210>  5
<211>  609
<212>  PRT
<213>  homo sapiens

<400>  5

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser Arg Gly Val Phe Arg Arg Asp Ala His Lys Ser Glu Val Ala 
            20                  25                  30          


His Arg Phe Lys Asp Leu Gly Glu Glu Asn Phe Lys Ala Leu Val Leu 
        35                  40                  45              


Ile Ala Phe Ala Gln Tyr Leu Gln Gln Cys Pro Phe Glu Asp His Val 
    50                  55                  60                  


Lys Leu Val Asn Glu Val Thr Glu Phe Ala Lys Thr Cys Val Ala Asp 
65                  70                  75                  80  


Glu Ser Ala Glu Asn Cys Asp Lys Ser Leu His Thr Leu Phe Gly Asp 
                85                  90                  95      


Lys Leu Cys Thr Val Ala Thr Leu Arg Glu Thr Tyr Gly Glu Met Ala 
            100                 105                 110         


Asp Cys Cys Ala Lys Gln Glu Pro Glu Arg Asn Glu Cys Phe Leu Gln 
        115                 120                 125             


His Lys Asp Asp Asn Pro Asn Leu Pro Arg Leu Val Arg Pro Glu Val 
    130                 135                 140                 


Asp Val Met Cys Thr Ala Phe His Asp Asn Glu Glu Thr Phe Leu Lys 
145                 150                 155                 160 


Lys Tyr Leu Tyr Glu Ile Ala Arg Arg His Pro Tyr Phe Tyr Ala Pro 
                165                 170                 175     


Glu Leu Leu Phe Phe Ala Lys Arg Tyr Lys Ala Ala Phe Thr Glu Cys 
            180                 185                 190         


Cys Gln Ala Ala Asp Lys Ala Ala Cys Leu Leu Pro Lys Leu Asp Glu 
        195                 200                 205             


Leu Arg Asp Glu Gly Lys Ala Ser Ser Ala Lys Gln Gly Leu Lys Cys 
    210                 215                 220                 


Ala Ser Leu Gln Lys Phe Gly Glu Arg Ala Phe Lys Ala Trp Ala Val 
225                 230                 235                 240 


Ala Arg Leu Ser Gln Arg Phe Pro Lys Ala Glu Phe Ala Glu Val Ser 
                245                 250                 255     


Lys Leu Val Thr Asp Leu Thr Lys Val His Thr Glu Cys Cys His Gly 
            260                 265                 270         


Asp Leu Leu Glu Cys Ala Asp Asp Arg Ala Asp Leu Ala Lys Tyr Ile 
        275                 280                 285             


Cys Glu Asn Gln Asp Ser Ile Ser Ser Lys Leu Lys Glu Cys Cys Glu 
    290                 295                 300                 


Lys Pro Leu Leu Glu Lys Ser His Cys Ile Ala Glu Val Glu Asn Asp 
305                 310                 315                 320 


Glu Met Pro Ala Asp Leu Pro Ser Leu Ala Ala Asp Phe Val Gly Ser 
                325                 330                 335     


Lys Asp Val Cys Lys Asn Tyr Ala Glu Ala Lys Asp Val Phe Leu Gly 
            340                 345                 350         


Met Phe Leu Tyr Glu Tyr Ala Arg Arg His Pro Asp Tyr Ser Val Val 
        355                 360                 365             


Leu Leu Leu Arg Leu Ala Lys Thr Tyr Glu Thr Thr Leu Glu Lys Cys 
    370                 375                 380                 


Cys Ala Ala Ala Asp Pro His Glu Cys Tyr Ala Lys Val Phe Asp Glu 
385                 390                 395                 400 


Phe Lys Pro Leu Val Glu Glu Pro Gln Asn Leu Ile Lys Gln Asn Cys 
                405                 410                 415     


Glu Leu Phe Glu Gln Leu Gly Glu Tyr Lys Phe Gln Asn Ala Leu Leu 
            420                 425                 430         


Val Arg Tyr Thr Lys Lys Val Pro Gln Val Ser Thr Pro Thr Leu Val 
        435                 440                 445             


Glu Val Ser Arg Asn Leu Gly Lys Val Gly Ser Lys Cys Cys Lys His 
    450                 455                 460                 


Pro Glu Ala Lys Arg Met Pro Cys Ala Glu Asp Cys Leu Ser Val Phe 
465                 470                 475                 480 


Leu Asn Gln Leu Cys Val Leu His Glu Lys Thr Pro Val Ser Asp Arg 
                485                 490                 495     


Val Thr Lys Cys Cys Thr Glu Ser Leu Val Asn Gly Arg Pro Cys Phe 
            500                 505                 510         


Ser Ala Leu Glu Val Asp Glu Thr Tyr Val Pro Lys Glu Phe Asn Ala 
        515                 520                 525             


Glu Thr Phe Thr Phe His Ala Asp Ile Cys Thr Leu Ser Glu Lys Glu 
    530                 535                 540                 


Arg Gln Ile Lys Lys Gln Thr Ala Leu Val Glu Leu Val Lys His Lys 
545                 550                 555                 560 


Pro Lys Ala Thr Lys Glu Gln Leu Lys Ala Val Met Asp Asp Phe Ala 
                565                 570                 575     


Ala Phe Val Glu Lys Cys Cys Lys Ala Asp Asp Lys Glu Thr Cys Phe 
            580                 585                 590         


Ala Glu Glu Gly Lys Lys Leu Val Ala Ala Ser Gln Ala Ala Leu Gly 
        595                 600                 605             


Leu 
    


<210>  6
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  C terminal sequence VH

<400>  6

Val Thr Val Ser Ser 
1               5   


<210>  7
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  His-tag

<400>  7

His His His His His His 
1               5       


<210>  8
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  8

Gly Gly Gly Gly Ser 
1               5   


<210>  9
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  9

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  10
<211>  10
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  10

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10  


<210>  11
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  11

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  


<210>  12
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  12

Gly Ser Gly Ser Gly Ser Gly Ser 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  13

Gly Gly Ser Gly Ser Gly Ser Gly 
1               5               


<210>  14
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  14

Gly Gly Ser Gly Ser Gly 
1               5       


<210>  15
<211>  4
<212>  PRT
<213>  artificial

<220>
<223>  linker

<400>  15

Gly Gly Ser Gly 
1               


<210>  16
<211>  750
<212>  DNA
<213>  homo sapiens

<400>  16
ggctttgtga gcggatacaa ttataatatg tggaattgtg agcgctcaca attccacaac       60

ggtttccctc tagaaataat tttgtttaac ttttaggagg taaaacatat gaagaaaacg      120

gcaatcgcaa tcgcagtcgc tctggcgggt ttcgcaactg tagcgcaagc cgaggtgcaa      180

ctggtcgagt ctggtggtgg tttggtgcaa cctggtagaa gcttgcgttt gagttgtgcc      240

gcttccggct tcactttcca tcattatgct atgcactggg ttcgtcaagc tcccggaaaa      300

ggtttggagt gggtttccgg aatttcctgg aatggcaata agattacgta cgctgattca      360

gtgaaaggaa ggtttacaat cagtagagat aatgctaaaa actcattgta tctacaaatg      420

aacagcctaa gagcagaaga taccgctctg tactactgtg ttagagatag ctcgttattc      480

attgtaggtg caccaacttt tgaacattgg ggtcggggta ctcttgtgac tgtctcatcc      540

gcggccgcac accaccatca tcaccactaa ctcgagcgcc taatgaaagc ttccccaagg      600

gcgacacccc ctaattagcc cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt      660

atttgatgcc tggcagttcc ctactctcgc atggggagtc cccacactac catcggcgct      720

acggcgtttc acttctgagt tcggcatgga                                       750


<210>  17
<211>  453
<212>  PRT
<213>  homo sapiens

<400>  17

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Phe Ser Gly Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Asp Pro Ala Trp Gly Leu Arg Leu Gly Glu Ser Ser Ser Tyr 
            100                 105                 110         


Asp Phe Asp Ile Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
145                 150                 155                 160 


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
                165                 170                 175     


Leu Ser Cys Ala Ala Ser Gly Phe Thr Leu Ser Asn Tyr Trp Met Asn 
            180                 185                 190         


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Asn Ile 
        195                 200                 205             


Asn Gln Asp Gly Ser Glu Arg Tyr Tyr Val Asp Ser Val Lys Gly Arg 
    210                 215                 220                 


Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met 
225                 230                 235                 240 


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly 
                245                 250                 255     


Gly Glu Gly Tyr Gly Val Asp His Tyr Gly Leu Asp Val Ser Gly Gln 
            260                 265                 270         


Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        275                 280                 285             


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    290                 295                 300                 


Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
305                 310                 315                 320 


Leu Val Gln Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
                325                 330                 335     


Phe Thr Phe His His Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly 
            340                 345                 350         


Lys Gly Leu Glu Trp Val Ser Gly Ile Ser Trp Asn Gly Asn Lys Ile 
        355                 360                 365             


Thr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
    370                 375                 380                 


Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
385                 390                 395                 400 


Thr Ala Leu Tyr Tyr Cys Val Arg Asp Ser Ser Leu Phe Ile Val Gly 
                405                 410                 415     


Ala Pro Thr Phe Glu His Trp Gly Arg Gly Thr Leu Val Thr Val Ser 
            420                 425                 430         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        435                 440                 445             


Gly Gly Gly Gly Ser 
    450             


<210>  18
<211>  1359
<212>  DNA
<213>  homo sapiens

<400>  18
gaggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc       60

tcctgtgcag cctctggatt ctccttcagt ggctatggca tgcactgggt ccgccaggct      120

ccaggcaagg gactggagtg ggtggcatat atatcatatg atggaagtaa taaatactat      180

gcagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat      240

ctgcaaatga acagcctgag agctgaggac acggctgtgt attactgtgc gaaagatccg      300

gcctggggat tacgtttggg ggagtcatcg tcctatgatt ttgatatctg gggccaaggg      360

acaatggtca ccgtctcctc aggtggtggc ggttcaggcg gaggtggctc tggaggtgga      420

ggttcaggag gtggtggttc tggcggcggt ggatcgggtg gaggtggtag tgaggtgcag      480

ttagttgaga gcggaggtgg tttagttcag ccggggggct cgcttcgcct gtcgtgcgcc      540

gcctcgggat tcacattatc aaactactgg atgaattggg tccgccaggc tccgggcaaa      600

ggtcttgagt gggtggcgaa cattaatcag gacgggagcg agcgttatta cgttgattcg      660

gtaaaaggac gtttcactat cagtcgtgac aacgctaaaa attccttgta cttacagatg      720

aactcacttc gtgctgagga caccgcagtg tactactgtg ctcgcggtgg tgaaggatac      780

ggcgtcgatc actacggcct tgatgtatca ggacagggga ctacagttac cgtctcttcc      840

ggcggaggtg gctctggagg aggcggatcg gggggtggag gaagtggcgg cggtggtagt      900

ggaggaggtg gttctggagg cggtggctct gaagtacaac tggttgaatc gggtggtgga      960

ttggtccaac ctggaagatc attgaggctt tcttgtgcag cttccggatt cacctttcat     1020

cactatgcta tgcactgggt gagacaagcc cctggtaagg gcttggaatg ggtgtccgga     1080

atctcctgga atggtaacaa aataacatat gcagattccg ttaagggtag atttactatt     1140

agccgtgata atgcaaaaaa cagtttatac ttgcagatga attccttgag ggctgaggat     1200

acagctcttt actattgtgt gcgtgactca tcgttgttca ttgtcggagc cccaactttc     1260

gaacattggg gtagaggtac cctagttacg gttagctcag gcggaggtgg ctctggagga     1320

ggcggatcgg ggggtggagg aagtggcggc ggtggtagt                            1359


<210>  19
<211>  123
<212>  PRT
<213>  homo sapiens

<400>  19

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Val Ser Gly Phe Thr Phe His His Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Trp Asn Gly Asn Lys Ile Thr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Val Arg Asp Ser Ser Leu Phe Ile Val Gly Ala Pro Thr Phe Asp His 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


<210>  20
<211>  123
<212>  PRT
<213>  homo sapiens

<400>  20

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Val Ser Gly Phe Thr Phe His His Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Gly Ile Ser Trp Asn Gly Asn Lys Ile Thr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Val Arg Asp Ser Ser Leu Phe Ile Val Gly Ala Pro Thr Phe Asp His 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120             


