                         SEQUENCE LISTING

<110>  Universitt Heidelberg
 
<120>  Relaxin Receptor 1 for use in Treatament and Prevention of Heart 
       Failure

<130>  UH15436PC

<150>  EP18207296.7
<151>  2018-11-20

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  2301
<212>  DNA
<213>  Artificial

<220>
<223>  codon-optimized human RXFP1 coding sequence

<400>  1
atggactaca aggacgacga tgacaagatg acatctggtt ctgtcttctt ctacatctta       60

atttttggaa aatatttttc tcatgggggt ggacaggatg tcaagtgctc ccttggctat      120

ttcccctgtg ggaacatcac aaagtgcttg cctcagctcc tgcactgtaa cggtgtggac      180

gactgcggga atcaggccga tgaggacaac tgtggagaca acaatggatg gtctctgcaa      240

tttgacaaat attttgccag ttactacaaa atgacttccc aatatccttt tgaggcagaa      300

acacctgaat gtttggtcgg ttctgtgcca gtgcaatgtc tttgccaagg tctggagctt      360

gactgtgatg aaaccaattt acgagctgtt ccatcggttt cttcaaatgt gactgcaatg      420

tcacttcagt ggaacttaat aagaaaactt cctcctgatt gcttcaagaa ttatcatgat      480

cttcagaagc tgtacctgca aaacaataag attacatcca tctccatcta tgctttcaga      540

ggactgaata gccttactaa actgtatctc agtcataaca gaataacctt cctgaagccg      600

ggtgtttttg aagatcttca cagactagaa tggctgataa ttgaagataa tcacctcagt      660

cgaatttccc caccaacatt ttatggacta aattctctta ttctcttagt cctgatgaat      720

aacgtcctca cccgtttacc tgataaacct ctctgtcaac acatgccaag actacattgg      780

ctggaccttg aaggcaacca tatccataat ttaagaaatt tgacttttat ttcctgtagt      840

aatttaactg ttttagtgat gaggaaaaac aaaattaatc acttaaatga aaatactttt      900

gcacctctcc agaaactgga tgaattggat ttaggaagta ataagattga aaatcttcca      960

ccgcttatat tcaaggacct gaaggagctg tcacaattga atctttccta taatccaatc     1020

cagaaaattc aagcaaacca atttgattat cttgtcaaac tcaagtctct cagcctagaa     1080

gggattgaaa tttcaaatat ccaacaaagg atgtttagac ctcttatgaa tctctctcac     1140

atatatttta agaaattcca gtactgtggg tatgcaccac atgttcgcag ctgtaaacca     1200

aacactgatg gaatttcatc tctagagaat ctcttggcaa gcattattca gagagtattt     1260

gtctgggttg tatctgctgt tacctgcttt ggaaacattt ttgtcatttg catgcgacct     1320

tatatcaggt ctgagaacaa gctgtatgcc atgtcaatca tttctctctg ctgtgccgac     1380

tgcttaatgg gaatatattt attcgtgatc ggaggctttg acctaaagtt tcgtggagaa     1440

tacaataagc atgcgcagct gtggatggag agtactcatt gtcagcttgt aggatctttg     1500

gccattctgt ccacagaagt atcagtttta ctgttaacat ttctgacatt ggaaaaatac     1560

atctgcattg tctatccttt tagatgtgtg agacctggaa aatgcagaac aattacagtt     1620

ctgattctca tttggattac tggttttata gtggctttca ttccattgag caataaggaa     1680

tttttcaaaa actactatgg caccaatgga gtatgcttcc ctcttcattc agaagataca     1740

gaaagtattg gagcccagat ttattcagtg gcaatttttc ttggtattaa tttggccgca     1800

tttatcatca tagttttttc ctatggaagc atgttttata gtgttcatca aagtgccata     1860

acagcaactg aaatacggaa tcaagttaaa aaagagatga tccttgccaa acgttttttc     1920

tttatagtat ttactgatgc attatgctgg atacccattt ttgtagtgaa atttctttca     1980

ctgcttcagg tagaaatacc aggtaccata acctcttggg tagtgatttt tattctgccc     2040

attaacagtg ctttgaaccc aattctctat actctgacca caagaccatt taaagaaatg     2100

attcatcggt tttggtataa ctacagacaa agaaaatcta tggacagcaa aggtcagaaa     2160

acatatgctc catcattcat ctgggtggaa atgtggccac tccaggagat gccacctgag     2220

ttaatgaagc cggacctttt cacatacccc tgtgaaatgt cactgatttc tcaatcaacg     2280

agactcaatt cctattcatg a                                               2301


<210>  2
<211>  766
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Asp Tyr Lys Asp Asp Asp Asp Lys Met Thr Ser Gly Ser Val Phe 
1               5                   10                  15      


Phe Tyr Ile Leu Ile Phe Gly Lys Tyr Phe Ser His Gly Gly Gly Gln 
            20                  25                  30          


Asp Val Lys Cys Ser Leu Gly Tyr Phe Pro Cys Gly Asn Ile Thr Lys 
        35                  40                  45              


Cys Leu Pro Gln Leu Leu His Cys Asn Gly Val Asp Asp Cys Gly Asn 
    50                  55                  60                  


Gln Ala Asp Glu Asp Asn Cys Gly Asp Asn Asn Gly Trp Ser Leu Gln 
65                  70                  75                  80  


Phe Asp Lys Tyr Phe Ala Ser Tyr Tyr Lys Met Thr Ser Gln Tyr Pro 
                85                  90                  95      


Phe Glu Ala Glu Thr Pro Glu Cys Leu Val Gly Ser Val Pro Val Gln 
            100                 105                 110         


Cys Leu Cys Gln Gly Leu Glu Leu Asp Cys Asp Glu Thr Asn Leu Arg 
        115                 120                 125             


Ala Val Pro Ser Val Ser Ser Asn Val Thr Ala Met Ser Leu Gln Trp 
    130                 135                 140                 


Asn Leu Ile Arg Lys Leu Pro Pro Asp Cys Phe Lys Asn Tyr His Asp 
145                 150                 155                 160 


Leu Gln Lys Leu Tyr Leu Gln Asn Asn Lys Ile Thr Ser Ile Ser Ile 
                165                 170                 175     


Tyr Ala Phe Arg Gly Leu Asn Ser Leu Thr Lys Leu Tyr Leu Ser His 
            180                 185                 190         


Asn Arg Ile Thr Phe Leu Lys Pro Gly Val Phe Glu Asp Leu His Arg 
        195                 200                 205             


Leu Glu Trp Leu Ile Ile Glu Asp Asn His Leu Ser Arg Ile Ser Pro 
    210                 215                 220                 


Pro Thr Phe Tyr Gly Leu Asn Ser Leu Ile Leu Leu Val Leu Met Asn 
225                 230                 235                 240 


Asn Val Leu Thr Arg Leu Pro Asp Lys Pro Leu Cys Gln His Met Pro 
                245                 250                 255     


Arg Leu His Trp Leu Asp Leu Glu Gly Asn His Ile His Asn Leu Arg 
            260                 265                 270         


Asn Leu Thr Phe Ile Ser Cys Ser Asn Leu Thr Val Leu Val Met Arg 
        275                 280                 285             


Lys Asn Lys Ile Asn His Leu Asn Glu Asn Thr Phe Ala Pro Leu Gln 
    290                 295                 300                 


Lys Leu Asp Glu Leu Asp Leu Gly Ser Asn Lys Ile Glu Asn Leu Pro 
305                 310                 315                 320 


Pro Leu Ile Phe Lys Asp Leu Lys Glu Leu Ser Gln Leu Asn Leu Ser 
                325                 330                 335     


Tyr Asn Pro Ile Gln Lys Ile Gln Ala Asn Gln Phe Asp Tyr Leu Val 
            340                 345                 350         


Lys Leu Lys Ser Leu Ser Leu Glu Gly Ile Glu Ile Ser Asn Ile Gln 
        355                 360                 365             


Gln Arg Met Phe Arg Pro Leu Met Asn Leu Ser His Ile Tyr Phe Lys 
    370                 375                 380                 


Lys Phe Gln Tyr Cys Gly Tyr Ala Pro His Val Arg Ser Cys Lys Pro 
385                 390                 395                 400 


Asn Thr Asp Gly Ile Ser Ser Leu Glu Asn Leu Leu Ala Ser Ile Ile 
                405                 410                 415     


Gln Arg Val Phe Val Trp Val Val Ser Ala Val Thr Cys Phe Gly Asn 
            420                 425                 430         


Ile Phe Val Ile Cys Met Arg Pro Tyr Ile Arg Ser Glu Asn Lys Leu 
        435                 440                 445             


Tyr Ala Met Ser Ile Ile Ser Leu Cys Cys Ala Asp Cys Leu Met Gly 
    450                 455                 460                 


Ile Tyr Leu Phe Val Ile Gly Gly Phe Asp Leu Lys Phe Arg Gly Glu 
465                 470                 475                 480 


Tyr Asn Lys His Ala Gln Leu Trp Met Glu Ser Thr His Cys Gln Leu 
                485                 490                 495     


Val Gly Ser Leu Ala Ile Leu Ser Thr Glu Val Ser Val Leu Leu Leu 
            500                 505                 510         


Thr Phe Leu Thr Leu Glu Lys Tyr Ile Cys Ile Val Tyr Pro Phe Arg 
        515                 520                 525             


Cys Val Arg Pro Gly Lys Cys Arg Thr Ile Thr Val Leu Ile Leu Ile 
    530                 535                 540                 


Trp Ile Thr Gly Phe Ile Val Ala Phe Ile Pro Leu Ser Asn Lys Glu 
545                 550                 555                 560 


Phe Phe Lys Asn Tyr Tyr Gly Thr Asn Gly Val Cys Phe Pro Leu His 
                565                 570                 575     


Ser Glu Asp Thr Glu Ser Ile Gly Ala Gln Ile Tyr Ser Val Ala Ile 
            580                 585                 590         


Phe Leu Gly Ile Asn Leu Ala Ala Phe Ile Ile Ile Val Phe Ser Tyr 
        595                 600                 605             


Gly Ser Met Phe Tyr Ser Val His Gln Ser Ala Ile Thr Ala Thr Glu 
    610                 615                 620                 


Ile Arg Asn Gln Val Lys Lys Glu Met Ile Leu Ala Lys Arg Phe Phe 
625                 630                 635                 640 


Phe Ile Val Phe Thr Asp Ala Leu Cys Trp Ile Pro Ile Phe Val Val 
                645                 650                 655     


Lys Phe Leu Ser Leu Leu Gln Val Glu Ile Pro Gly Thr Ile Thr Ser 
            660                 665                 670         


Trp Val Val Ile Phe Ile Leu Pro Ile Asn Ser Ala Leu Asn Pro Ile 
        675                 680                 685             


Leu Tyr Thr Leu Thr Thr Arg Pro Phe Lys Glu Met Ile His Arg Phe 
    690                 695                 700                 


Trp Tyr Asn Tyr Arg Gln Arg Lys Ser Met Asp Ser Lys Gly Gln Lys 
705                 710                 715                 720 


Thr Tyr Ala Pro Ser Phe Ile Trp Val Glu Met Trp Pro Leu Gln Glu 
                725                 730                 735     


Met Pro Pro Glu Leu Met Lys Pro Asp Leu Phe Thr Tyr Pro Cys Glu 
            740                 745                 750         


Met Ser Leu Ile Ser Gln Ser Thr Arg Leu Asn Ser Tyr Ser 
        755                 760                 765     


<210>  3
<211>  2274
<212>  DNA
<213>  Artificial

<220>
<223>  5' shortened codon-optimized human RXFP1 coding sequence

<400>  3
atgacatctg gttctgtctt cttctacatc ttaatttttg gaaaatattt ttctcatggg       60

ggtggacagg atgtcaagtg ctcccttggc tatttcccct gtgggaacat cacaaagtgc      120

ttgcctcagc tcctgcactg taacggtgtg gacgactgcg ggaatcaggc cgatgaggac      180

aactgtggag acaacaatgg atggtctctg caatttgaca aatattttgc cagttactac      240

aaaatgactt cccaatatcc ttttgaggca gaaacacctg aatgtttggt cggttctgtg      300

ccagtgcaat gtctttgcca aggtctggag cttgactgtg atgaaaccaa tttacgagct      360

gttccatcgg tttcttcaaa tgtgactgca atgtcacttc agtggaactt aataagaaag      420

cttcctcctg attgcttcaa gaattatcat gatcttcaga agctgtacct gcaaaacaat      480

aagattacat ccatctccat ctatgctttc agaggactga atagccttac taaactgtat      540

ctcagtcata acagaataac cttcctgaag ccgggtgttt ttgaagatct tcacagacta      600

gaatggctga taattgaaga taatcacctc agtcgaattt ccccaccaac attttatgga      660

ctaaattctc ttattctctt agtcctgatg aataacgtcc tcacccgttt acctgataaa      720

cctctctgtc aacacatgcc aagactacat tggctggacc ttgaaggcaa ccatatccat      780

aatttaagaa atttgacttt tatttcctgc agtaatttaa ctgttttagt gatgaggaaa      840

aacaaaatta atcacttaaa tgaaaatact tttgcacctc tccagaaact ggatgaattg      900

gatttaggaa gtaataagat tgaaaatctt ccaccgctta tattcaagga cctgaaggag      960

ctgtcacaat tgaatctttc ctataatcca atccagaaaa ttcaagcaaa ccaatttgat     1020

tatcttgtca aactcaagtc tctcagccta gaagggattg aaatttcaaa tatccaacaa     1080

aggatgttta gacctcttat gaatctctct cacatatatt ttaagaaatt ccagtactgt     1140

gggtatgcac cacatgttcg cagctgtaaa ccaaacactg atggaatttc atctctagag     1200

aatctcttgg caagcattat tcagagagta tttgtctggg ttgtatctgc agttacctgc     1260

tttggaaaca tttttgtcat ttgcatgcga ccttatatca ggtctgagaa caagctgtat     1320

gccatgtcaa tcatttctct ctgctgtgcc gactgcttaa tgggaatata tttattcgtg     1380

atcggaggct ttgacctaaa gtttcgtgga gaatacaata agcatgcgca gctgtggatg     1440

gagagtactc attgtcagct tgtaggatct ttggccattc tgtccacaga agtatcagtt     1500

ttactgttaa catttctgac attggaaaaa tacatctgca ttgtctatcc ttttagatgt     1560

gtgagacctg gaaaatgcag aacaattaca gttctgattc tcatttggat tactggtttt     1620

atagtggctt tcattccatt gagcaataag gaatttttca aaaactacta tggcaccaat     1680

ggagtatgct tccctcttca ttcagaagat acagaaagta ttggagccca gatttattca     1740

gtggcaattt ttcttggtat taatttggcc gcatttatca tcatagtttt ttcctatgga     1800

agcatgtttt atagtgttca tcaaagtgcc ataacagcaa ctgaaatacg gaatcaagtt     1860

aaaaaagaga tgatccttgc caaacgtttt ttctttatag tatttactga tgcattatgc     1920

tggataccca tttttgtagt gaaatttctt tcactgcttc aggtagaaat accaggtacc     1980

ataacctctt gggtagtgat ttttattctg cccattaaca gtgctttgaa cccaattctc     2040

tatactctga ccacaagacc atttaaagaa atgattcatc ggttttggta taactacaga     2100

caaagaaaat ctatggacag caaaggtcag aaaacatatg ctccatcatt catctgggtg     2160

gaaatgtggc cactgcagga gatgccacct gagttaatga agccggacct tttcacatac     2220

ccctgtgaaa tgtcactgat ttctcaatca acgagactca attcctattc atga           2274


<210>  4
<211>  757
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Thr Ser Gly Ser Val Phe Phe Tyr Ile Leu Ile Phe Gly Lys Tyr 
1               5                   10                  15      


Phe Ser His Gly Gly Gly Gln Asp Val Lys Cys Ser Leu Gly Tyr Phe 
            20                  25                  30          


Pro Cys Gly Asn Ile Thr Lys Cys Leu Pro Gln Leu Leu His Cys Asn 
        35                  40                  45              


Gly Val Asp Asp Cys Gly Asn Gln Ala Asp Glu Asp Asn Cys Gly Asp 
    50                  55                  60                  


Asn Asn Gly Trp Ser Leu Gln Phe Asp Lys Tyr Phe Ala Ser Tyr Tyr 
65                  70                  75                  80  


Lys Met Thr Ser Gln Tyr Pro Phe Glu Ala Glu Thr Pro Glu Cys Leu 
                85                  90                  95      


Val Gly Ser Val Pro Val Gln Cys Leu Cys Gln Gly Leu Glu Leu Asp 
            100                 105                 110         


Cys Asp Glu Thr Asn Leu Arg Ala Val Pro Ser Val Ser Ser Asn Val 
        115                 120                 125             


Thr Ala Met Ser Leu Gln Trp Asn Leu Ile Arg Lys Leu Pro Pro Asp 
    130                 135                 140                 


Cys Phe Lys Asn Tyr His Asp Leu Gln Lys Leu Tyr Leu Gln Asn Asn 
145                 150                 155                 160 


Lys Ile Thr Ser Ile Ser Ile Tyr Ala Phe Arg Gly Leu Asn Ser Leu 
                165                 170                 175     


Thr Lys Leu Tyr Leu Ser His Asn Arg Ile Thr Phe Leu Lys Pro Gly 
            180                 185                 190         


Val Phe Glu Asp Leu His Arg Leu Glu Trp Leu Ile Ile Glu Asp Asn 
        195                 200                 205             


His Leu Ser Arg Ile Ser Pro Pro Thr Phe Tyr Gly Leu Asn Ser Leu 
    210                 215                 220                 


Ile Leu Leu Val Leu Met Asn Asn Val Leu Thr Arg Leu Pro Asp Lys 
225                 230                 235                 240 


Pro Leu Cys Gln His Met Pro Arg Leu His Trp Leu Asp Leu Glu Gly 
                245                 250                 255     


Asn His Ile His Asn Leu Arg Asn Leu Thr Phe Ile Ser Cys Ser Asn 
            260                 265                 270         


Leu Thr Val Leu Val Met Arg Lys Asn Lys Ile Asn His Leu Asn Glu 
        275                 280                 285             


Asn Thr Phe Ala Pro Leu Gln Lys Leu Asp Glu Leu Asp Leu Gly Ser 
    290                 295                 300                 


Asn Lys Ile Glu Asn Leu Pro Pro Leu Ile Phe Lys Asp Leu Lys Glu 
305                 310                 315                 320 


Leu Ser Gln Leu Asn Leu Ser Tyr Asn Pro Ile Gln Lys Ile Gln Ala 
                325                 330                 335     


Asn Gln Phe Asp Tyr Leu Val Lys Leu Lys Ser Leu Ser Leu Glu Gly 
            340                 345                 350         


Ile Glu Ile Ser Asn Ile Gln Gln Arg Met Phe Arg Pro Leu Met Asn 
        355                 360                 365             


Leu Ser His Ile Tyr Phe Lys Lys Phe Gln Tyr Cys Gly Tyr Ala Pro 
    370                 375                 380                 


His Val Arg Ser Cys Lys Pro Asn Thr Asp Gly Ile Ser Ser Leu Glu 
385                 390                 395                 400 


Asn Leu Leu Ala Ser Ile Ile Gln Arg Val Phe Val Trp Val Val Ser 
                405                 410                 415     


Ala Val Thr Cys Phe Gly Asn Ile Phe Val Ile Cys Met Arg Pro Tyr 
            420                 425                 430         


Ile Arg Ser Glu Asn Lys Leu Tyr Ala Met Ser Ile Ile Ser Leu Cys 
        435                 440                 445             


Cys Ala Asp Cys Leu Met Gly Ile Tyr Leu Phe Val Ile Gly Gly Phe 
    450                 455                 460                 


Asp Leu Lys Phe Arg Gly Glu Tyr Asn Lys His Ala Gln Leu Trp Met 
465                 470                 475                 480 


Glu Ser Thr His Cys Gln Leu Val Gly Ser Leu Ala Ile Leu Ser Thr 
                485                 490                 495     


Glu Val Ser Val Leu Leu Leu Thr Phe Leu Thr Leu Glu Lys Tyr Ile 
            500                 505                 510         


Cys Ile Val Tyr Pro Phe Arg Cys Val Arg Pro Gly Lys Cys Arg Thr 
        515                 520                 525             


Ile Thr Val Leu Ile Leu Ile Trp Ile Thr Gly Phe Ile Val Ala Phe 
    530                 535                 540                 


Ile Pro Leu Ser Asn Lys Glu Phe Phe Lys Asn Tyr Tyr Gly Thr Asn 
545                 550                 555                 560 


Gly Val Cys Phe Pro Leu His Ser Glu Asp Thr Glu Ser Ile Gly Ala 
                565                 570                 575     


Gln Ile Tyr Ser Val Ala Ile Phe Leu Gly Ile Asn Leu Ala Ala Phe 
            580                 585                 590         


Ile Ile Ile Val Phe Ser Tyr Gly Ser Met Phe Tyr Ser Val His Gln 
        595                 600                 605             


Ser Ala Ile Thr Ala Thr Glu Ile Arg Asn Gln Val Lys Lys Glu Met 
    610                 615                 620                 


Ile Leu Ala Lys Arg Phe Phe Phe Ile Val Phe Thr Asp Ala Leu Cys 
625                 630                 635                 640 


Trp Ile Pro Ile Phe Val Val Lys Phe Leu Ser Leu Leu Gln Val Glu 
                645                 650                 655     


Ile Pro Gly Thr Ile Thr Ser Trp Val Val Ile Phe Ile Leu Pro Ile 
            660                 665                 670         


Asn Ser Ala Leu Asn Pro Ile Leu Tyr Thr Leu Thr Thr Arg Pro Phe 
        675                 680                 685             


Lys Glu Met Ile His Arg Phe Trp Tyr Asn Tyr Arg Gln Arg Lys Ser 
    690                 695                 700                 


Met Asp Ser Lys Gly Gln Lys Thr Tyr Ala Pro Ser Phe Ile Trp Val 
705                 710                 715                 720 


Glu Met Trp Pro Leu Gln Glu Met Pro Pro Glu Leu Met Lys Pro Asp 
                725                 730                 735     


Leu Phe Thr Tyr Pro Cys Glu Met Ser Leu Ile Ser Gln Ser Thr Arg 
            740                 745                 750         


Leu Asn Ser Tyr Ser 
        755         


<210>  5
<211>  2274
<212>  DNA
<213>  Homo sapiens

<400>  5
atgacatctg gttctgtctt cttctacatc ttaatttttg gaaaatattt ttctcatggg       60

ggtggacagg atgtcaagtg ctcccttggc tatttcccct gtgggaacat cacaaagtgc      120

ttgcctcagc tcctgcactg taacggtgtg gacgactgcg ggaatcaggc cgatgaggac      180

aactgtggag acaacaatgg atggtctctg caatttgaca aatattttgc cagttactac      240

aaaatgactt cccaatatcc ttttgaggca gaaacacctg aatgtttggt cggttctgtg      300

ccagtgcaat gtctttgcca aggtctggag cttgactgtg atgaaaccaa tttacgagct      360

gttccatcgg tttcttcaaa tgtgactgca atgtcacttc agtggaactt aataagaaag      420

cttcctcctg attgcttcaa gaattatcat gatcttcaga agctgtacct gcaaaacaat      480

aagattacat ccatctccat ctatgctttc agaggactga atagccttac taaactgtat      540

ctcagtcata acagaataac cttcctgaag ccgggtgttt ttgaagatct tcacagacta      600

gaatggctga taattgaaga taatcacctc agtcgaattt ccccaccaac attttatgga      660

ctaaattctc ttattctctt agtcctgatg aataacgtcc tcacccgttt acctgataaa      720

cctctctgtc aacacatgcc aagactacat tggctggacc ttgaaggcaa ccatatccat      780

aatttaagaa atttgacttt tatttcctgc agtaatttaa ctgttttagt gatgaggaaa      840

aacaaaatta atcacttaaa tgaaaatact tttgcacctc tccagaaact ggatgaattg      900

gatttaggaa gtaataagat tgaaaatctt ccaccgctta tattcaagga cctgaaggag      960

ctgtcacaat tgaatctttc ctataatcca atccagaaaa ttcaagcaaa ccaatttgat     1020

tatcttgtca aactcaagtc tctcagccta gaagggattg aaatttcaaa tatccaacaa     1080

aggatgttta gacctcttat gaatctctct cacatatatt ttaagaaatt ccagtactgt     1140

gggtatgcac cacatgttcg cagctgtaaa ccaaacactg atggaatttc atctctagag     1200

aatctcttgg caagcattat tcagagagta tttgtctggg ttgtatctgc agttacctgc     1260

tttggaaaca tttttgtcat ttgcatgcga ccttatatca ggtctgagaa caagctgtat     1320

gccatgtcaa tcatttctct ctgctgtgcc gactgcttaa tgggaatata tttattcgtg     1380

atcggaggct ttgacctaaa gtttcgtgga gaatacaata agcatgcgca gctgtggatg     1440

gagagtactc attgtcagct tgtaggatct ttggccattc tgtccacaga agtatcagtt     1500

ttactgttaa catttctgac attggaaaaa tacatctgca ttgtctatcc ttttagatgt     1560

gtgagacctg gaaaatgcag aacaattaca gttctgattc tcatttggat tactggtttt     1620

atagtggctt tcattccatt gagcaataag gaatttttca aaaactacta tggcaccaat     1680

ggagtatgct tccctcttca ttcagaagat acagaaagta ttggagccca gatttattca     1740

gtggcaattt ttcttggtat taatttggcc gcatttatca tcatagtttt ttcctatgga     1800

agcatgtttt atagtgttca tcaaagtgcc ataacagcaa ctgaaatacg gaatcaagtt     1860

aaaaaagaga tgatccttgc caaacgtttt ttctttatag tatttactga tgcattatgc     1920

tggataccca tttttgtagt gaaatttctt tcactgcttc aggtagaaat accaggtacc     1980

ataacctctt gggtagtgat ttttattctg cccattaaca gtgctttgaa cccaattctc     2040

tatactctga ccacaagacc atttaaagaa atgattcatc ggttttggta taactacaga     2100

caaagaaaat ctatggacag caaaggtcag aaaacatatg ctccatcatt catctgggtg     2160

gaaatgtggc cactgcagga gatgccacct gagttaatga agccggacct tttcacatac     2220

ccctgtgaaa tgtcactgat ttctcaatca acgagactca attcctattc atga           2274


<210>  6
<211>  518
<212>  DNA
<213>  Artificial

<220>
<223>  CMV enhancer

<400>  6
actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc       60

cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca      120

ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt      180

caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg      240

ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag      300

tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt      360

accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg      420

ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa      480

cgggactttc caaaatgtcg taacactccg ccccatgc                              518


<210>  7
<211>  263
<212>  DNA
<213>  Artificial

<220>
<223>  MLC260 promoter

<400>  7
gacccagagc acagagcatc gttcccaggc caggccccag ccactgtctc tttaaccttg       60

aaggcatttt tgggtctcac gtgtccaccc aggcgggtgt cggactttga acggctctta      120

cttcagaaga acggcatggg gtgggggggc ttaggtggcc tctgcctcac ctacaactgc      180

caaaagtggt catggggtta tttttaaccc cagggaagag gtatttattg ttccacagca      240

ggggccggcc agcaggctcc ttg                                              263


<210>  8
<211>  3112
<212>  DNA
<213>  Artificial

<220>
<223>  CMV-MLC260 -Modified Human RXFP1

<400>  8
actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc       60

cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca      120

ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt      180

caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg      240

ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag      300

tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt      360

accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg      420

ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa      480

cgggactttc caaaatgtcg taacactccg ccccatgcgg ccgctctagg acccagagca      540

cagagcatcg ttcccaggcc aggccccagc cactgtctct ttaaccttga aggcattttt      600

gggtctcacg tgtccaccca ggcgggtgtc ggactttgaa cggctcttac ttcagaagaa      660

cggcatgggg tgggggggct taggtggcct ctgcctcacc tacaactgcc aaaagtggtc      720

atggggttat ttttaacccc agggaagagg tatttattgt tccacagcag gggccggcca      780

gcaggctcct tgaattctgc agaccatgga ctacaaggac gacgatgaca agatgacatc      840

tggttctgtc ttcttctaca tcttaatttt tggaaaatat ttttctcatg ggggtggaca      900

ggatgtcaag tgctcccttg gctatttccc ctgtgggaac atcacaaagt gcttgcctca      960

gctcctgcac tgtaacggtg tggacgactg cgggaatcag gccgatgagg acaactgtgg     1020

agacaacaat ggatggtctc tgcaatttga caaatatttt gccagttact acaaaatgac     1080

ttcccaatat ccttttgagg cagaaacacc tgaatgtttg gtcggttctg tgccagtgca     1140

atgtctttgc caaggtctgg agcttgactg tgatgaaacc aatttacgag ctgttccatc     1200

ggtttcttca aatgtgactg caatgtcact tcagtggaac ttaataagaa aacttcctcc     1260

tgattgcttc aagaattatc atgatcttca gaagctgtac ctgcaaaaca ataagattac     1320

atccatctcc atctatgctt tcagaggact gaatagcctt actaaactgt atctcagtca     1380

taacagaata accttcctga agccgggtgt ttttgaagat cttcacagac tagaatggct     1440

gataattgaa gataatcacc tcagtcgaat ttccccacca acattttatg gactaaattc     1500

tcttattctc ttagtcctga tgaataacgt cctcacccgt ttacctgata aacctctctg     1560

tcaacacatg ccaagactac attggctgga ccttgaaggc aaccatatcc ataatttaag     1620

aaatttgact tttatttcct gtagtaattt aactgtttta gtgatgagga aaaacaaaat     1680

taatcactta aatgaaaata cttttgcacc tctccagaaa ctggatgaat tggatttagg     1740

aagtaataag attgaaaatc ttccaccgct tatattcaag gacctgaagg agctgtcaca     1800

attgaatctt tcctataatc caatccagaa aattcaagca aaccaatttg attatcttgt     1860

caaactcaag tctctcagcc tagaagggat tgaaatttca aatatccaac aaaggatgtt     1920

tagacctctt atgaatctct ctcacatata ttttaagaaa ttccagtact gtgggtatgc     1980

accacatgtt cgcagctgta aaccaaacac tgatggaatt tcatctctag agaatctctt     2040

ggcaagcatt attcagagag tatttgtctg ggttgtatct gctgttacct gctttggaaa     2100

catttttgtc atttgcatgc gaccttatat caggtctgag aacaagctgt atgccatgtc     2160

aatcatttct ctctgctgtg ccgactgctt aatgggaata tatttattcg tgatcggagg     2220

ctttgaccta aagtttcgtg gagaatacaa taagcatgcg cagctgtgga tggagagtac     2280

tcattgtcag cttgtaggat ctttggccat tctgtccaca gaagtatcag ttttactgtt     2340

aacatttctg acattggaaa aatacatctg cattgtctat ccttttagat gtgtgagacc     2400

tggaaaatgc agaacaatta cagttctgat tctcatttgg attactggtt ttatagtggc     2460

tttcattcca ttgagcaata aggaattttt caaaaactac tatggcacca atggagtatg     2520

cttccctctt cattcagaag atacagaaag tattggagcc cagatttatt cagtggcaat     2580

ttttcttggt attaatttgg ccgcatttat catcatagtt ttttcctatg gaagcatgtt     2640

ttatagtgtt catcaaagtg ccataacagc aactgaaata cggaatcaag ttaaaaaaga     2700

gatgatcctt gccaaacgtt ttttctttat agtatttact gatgcattat gctggatacc     2760

catttttgta gtgaaatttc tttcactgct tcaggtagaa ataccaggta ccataacctc     2820

ttgggtagtg atttttattc tgcccattaa cagtgctttg aacccaattc tctatactct     2880

gaccacaaga ccatttaaag aaatgattca tcggttttgg tataactaca gacaaagaaa     2940

atctatggac agcaaaggtc agaaaacata tgctccatca ttcatctggg tggaaatgtg     3000

gccactccag gagatgccac ctgagttaat gaagccggac cttttcacat acccctgtga     3060

aatgtcactg atttctcaat caacgagact caattcctat tcatgactcg ag             3112


<210>  9
<211>  185
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Pro Arg Leu Phe Leu Phe His Leu Leu Glu Phe Cys Leu Leu Leu 
1               5                   10                  15      


Asn Gln Phe Ser Arg Ala Val Ala Ala Lys Trp Lys Asp Asp Val Ile 
            20                  25                  30          


Lys Leu Cys Gly Arg Glu Leu Val Arg Ala Gln Ile Ala Ile Cys Gly 
        35                  40                  45              


Met Ser Thr Trp Ser Lys Arg Ser Leu Ser Gln Glu Asp Ala Pro Gln 
    50                  55                  60                  


Thr Pro Arg Pro Val Ala Glu Ile Val Pro Ser Phe Ile Asn Lys Asp 
65                  70                  75                  80  


Thr Glu Thr Ile Ile Ile Met Leu Glu Phe Ile Ala Asn Leu Pro Pro 
                85                  90                  95      


Glu Leu Lys Ala Ala Leu Ser Glu Arg Gln Pro Ser Leu Pro Glu Leu 
            100                 105                 110         


Gln Gln Tyr Val Pro Ala Leu Lys Asp Ser Asn Leu Ser Phe Glu Glu 
        115                 120                 125             


Phe Lys Lys Leu Ile Arg Asn Arg Gln Ser Glu Ala Ala Asp Ser Asn 
    130                 135                 140                 


Pro Ser Glu Leu Lys Tyr Leu Gly Leu Asp Thr His Ser Gln Lys Lys 
145                 150                 155                 160 


Arg Arg Pro Tyr Val Ala Leu Phe Glu Lys Cys Cys Leu Ile Gly Cys 
                165                 170                 175     


Thr Lys Arg Ser Leu Ala Lys Tyr Cys 
            180                 185 


<210>  10
<211>  29
<212>  PRT
<213>  Artificial

<220>
<223>  Serelaxin heavy chain

<400>  10

Asp Ser Trp Met Glu Glu Val Ile Lys Leu Cys Gly Arg Glu Leu Val 
1               5                   10                  15      


Arg Ala Gln Ile Ala Ile Cys Gly Met Ser Thr Trp Ser 
            20                  25                  


<210>  11
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  Serelaxin light chain

<400>  11

Gln Leu Tyr Ser Ala Leu Ala Asn Lys Cys Cys His Val Gly Cys Thr 
1               5                   10                  15      


Lys Arg Ser Leu Ala Arg Phe Cys 
            20                  


<210>  12
<211>  18
<212>  PRT
<213>  Artificial

<220>
<223>  short serelaxin heavy chain

<400>  12

Val Ile Lys Leu Cys Gly Arg Glu Leu Val Arg Ala Gln Ile Ala Ile 
1               5                   10                  15      


Cys Gly 
        


<210>  13
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  short serelaxin light chain

<400>  13

Ser Ala Leu Ala Asn Lys Cys Cys His Val Gly Cys Thr Lys Arg Ser 
1               5                   10                  15      


Leu Ala Arg Phe Cys 
            20      


<210>  14
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  ANP Forward primer

<400>  14
tgccggtaga agatgaggtc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  ANP Reverse Primer

<400>  15
tgcttttcaa gagggcagat                                                   20


<210>  16
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  beta-MHC Forward primer

<400>  16
gccaacacca acctgtccaa gttc                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  beta-MHC Reverse primer

<400>  17
tgcaaaggct ccaggtctga gggc                                              24


<210>  18
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  BNP Forward primer

<400>  18
ctgaaggtgc tgtcccagat                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  BNP Reverse primer

<400>  19
ccttggtcct tcaagagctg                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Col1a1 Forward primer

<400>  20
gtgttcccta ctcagccgtc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Col1a1 Reverse Primer

<400>  21
actcgaacgg gaatccatcg                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Col3a1 Forward primer

<400>  22
tgactgtccc acgtaagcac                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Col3a1 Reverse primer

<400>  23
gagggccata gctgaactga                                                   20


<210>  24
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Hprt1 Forward primer

<400>  24
gaggagtcct gttgatgttg ccag                                              24


<210>  25
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Hprt1 Reverse primer

<400>  25
ggctggccta taggctcata gtgc                                              24


<210>  26
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  Postn Forward primer

<400>  26
acaaaagggt tcaagggcct a                                                 21


<210>  27
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Postn Reverse primer

<400>  27
ttggcttctg ttggttgtca                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Rattus norvegicus

<400>  28
ccatgcattg tttgtgccga                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Rattus norvegicus

<400>  29
tttgcaggca cagcttttgg                                                   20


<210>  30
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  30
ctacaaggac gacgatgaca ag                                                22


<210>  31
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  31
gaaatagcca agggagcact tg                                                22


<210>  32
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  32
ctttcctggg aggtcgttcc                                                   20


<210>  33
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  33
gttgcgctgc tcctgtaac                                                    19


<210>  34
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  34
ctcatggact aattatggac aggac                                             25


<210>  35
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  35
gcaggtcagc aaagaattta tagcc                                             25


<210>  36
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  36
ggacctgaag gagctgtcac                                                   20


<210>  37
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  37
taggctgaga gacttgagtt tgac                                              24


<210>  38
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  38

Met Ala Arg Tyr Met Leu Leu Leu Leu Leu Ala Val Trp Val Leu Thr 
1               5                   10                  15      


Gly Glu Leu Trp Pro Gly Ala Glu Ala Arg Ala Ala Pro Tyr Gly Val 
            20                  25                  30          


Arg Leu Cys Gly Arg Glu Phe Ile Arg Ala Val Ile Phe Thr Cys Gly 
        35                  40                  45              


Gly Ser Arg Trp Arg Arg Ser Asp Ile Leu Ala His Glu Ala Met Gly 
    50                  55                  60                  


Asp Thr Phe Pro Asp Ala Asp Ala Asp Glu Asp Ser Leu Ala Gly Glu 
65                  70                  75                  80  


Leu Asp Glu Ala Met Gly Ser Ser Glu Trp Leu Ala Leu Thr Lys Ser 
                85                  90                  95      


Pro Gln Ala Phe Tyr Arg Gly Arg Pro Ser Trp Gln Gly Thr Pro Gly 
            100                 105                 110         


Val Leu Arg Gly Ser Arg Asp Val Leu Ala Gly Leu Ser Ser Ser Cys 
        115                 120                 125             


Cys Lys Trp Gly Cys Ser Lys Ser Glu Ile Ser Ser Leu Cys 
    130                 135                 140         


