                               SEQUENCE LISTING

<110> GRO BIOSCIENCES INC.
 
<120> HUMAN DNASE FOR LUNG DISEASE

<130> 52252-707.602

<140> PCT/US2019/062199
<141> 2019-11-19

<150> 62/769,258
<151> 2018-11-19

<160> 30    

<170> PatentIn version 3.5

<210> 1
<211> 282
<212> PRT
<213> Homo sapiens

<300>
<308> AAA63170.1, GenBank
<309> 1995-03-06

<400> 1
Met Arg Gly Met Lys Leu Leu Gly Ala Leu Leu Ala Leu Ala Ala Leu 
1               5                   10                  15      


Leu Gln Gly Ala Val Ser Leu Lys Ile Ala Ala Phe Asn Ile Gln Thr 
            20                  25                  30          


Phe Gly Glu Thr Lys Met Ser Asn Ala Thr Leu Val Ser Tyr Ile Val 
        35                  40                  45              


Gln Ile Leu Ser Arg Tyr Asp Ile Ala Leu Val Gln Glu Val Arg Asp 
    50                  55                  60                  


Ser His Leu Thr Ala Val Gly Lys Leu Leu Asp Asn Leu Asn Gln Asp 
65                  70                  75                  80  


Ala Pro Asp Thr Tyr His Tyr Val Val Ser Glu Pro Leu Gly Arg Asn 
                85                  90                  95      


Ser Tyr Lys Glu Arg Tyr Leu Phe Val Tyr Arg Pro Asp Gln Val Ser 
            100                 105                 110         


Ala Val Asp Ser Tyr Tyr Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn 
        115                 120                 125             


Asp Thr Phe Asn Arg Glu Pro Ala Ile Val Arg Phe Phe Ser Arg Phe 
    130                 135                 140                 


Thr Glu Val Arg Glu Phe Ala Ile Val Pro Leu His Ala Ala Pro Gly 
145                 150                 155                 160 


Asp Ala Val Ala Glu Ile Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val 
                165                 170                 175     


Gln Glu Lys Trp Gly Leu Glu Asp Val Met Leu Met Gly Asp Phe Asn 
            180                 185                 190         


Ala Gly Cys Ser Tyr Val Arg Pro Ser Gln Trp Ser Ser Ile Arg Leu 
        195                 200                 205             


Trp Thr Ser Pro Thr Phe Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr 
    210                 215                 220                 


Thr Ala Thr Pro Thr His Cys Ala Tyr Asp Arg Ile Val Val Ala Gly 
225                 230                 235                 240 


Met Leu Leu Arg Gly Ala Val Val Pro Asp Ser Ala Leu Pro Phe Asn 
                245                 250                 255     


Phe Gln Ala Ala Tyr Gly Leu Ser Asp Gln Leu Ala Gln Ala Ile Ser 
            260                 265                 270         


Asp His Tyr Pro Val Glu Val Met Leu Lys 
        275                 280         


<210> 2
<211> 282
<212> PRT
<213> Homo sapiens

<300>
<308> CAC12813.1, GenBank
<309> 2008-10-07

<400> 2
Met Arg Gly Met Lys Leu Leu Gly Ala Leu Leu Ala Leu Ala Ala Leu 
1               5                   10                  15      


Leu Gln Gly Ala Val Ser Leu Lys Ile Ala Ala Phe Asn Ile Gln Thr 
            20                  25                  30          


Phe Gly Glu Thr Lys Met Ser Asn Ala Thr Leu Val Ser Tyr Ile Val 
        35                  40                  45              


Gln Ile Leu Ser Arg Tyr Asp Ile Ala Leu Val Gln Glu Val Arg Asp 
    50                  55                  60                  


Ser His Leu Thr Ala Val Gly Lys Leu Leu Asp Asn Leu Asn Gln Asp 
65                  70                  75                  80  


Ala Pro Asp Thr Tyr His Tyr Val Val Ser Glu Pro Leu Gly Arg Asn 
                85                  90                  95      


Ser Tyr Lys Glu Arg Tyr Leu Phe Val Tyr Arg Pro Asp Gln Val Ser 
            100                 105                 110         


Ala Val Asp Ser Tyr Tyr Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn 
        115                 120                 125             


Asp Thr Phe Asn Arg Glu Pro Ala Ile Val Arg Phe Phe Ser Arg Phe 
    130                 135                 140                 


Thr Glu Val Arg Glu Phe Ala Ile Val Pro Leu His Ala Ala Pro Gly 
145                 150                 155                 160 


Asp Ala Val Ala Glu Ile Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val 
                165                 170                 175     


Gln Glu Lys Trp Gly Leu Glu Asp Val Met Leu Met Gly Asp Phe Asn 
            180                 185                 190         


Ala Gly Cys Ser Tyr Val Arg Pro Ser Gln Trp Ser Ser Ile Arg Leu 
        195                 200                 205             


Trp Thr Ser Pro Thr Phe Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr 
    210                 215                 220                 


Thr Ala Thr Pro Thr His Cys Ala Tyr Asp Arg Ile Val Val Ala Gly 
225                 230                 235                 240 


Met Leu Leu Arg Gly Ala Val Val Pro Asp Ser Ala Leu Pro Phe Asn 
                245                 250                 255     


Phe Gln Ala Ala Tyr Gly Leu Ser Asp Gln Leu Ala Gln Ala Ile Ser 
            260                 265                 270         


Asp His Tyr Pro Val Glu Val Met Leu Lys 
        275                 280         


<210> 3
<211> 302
<212> PRT
<213> Homo sapiens

<300>
<308> AAB00495.1, GenBank
<309> 1996-05-21

<400> 3
Met His Tyr Pro Thr Ala Leu Leu Phe Leu Ile Leu Ala Asn Gly Ala 
1               5                   10                  15      


Gln Ala Phe Arg Ile Cys Ala Phe Asn Ala Gln Arg Leu Thr Leu Ala 
            20                  25                  30          


Lys Val Ala Arg Glu Gln Val Met Asp Thr Leu Val Arg Ile Leu Ala 
        35                  40                  45              


Arg Cys Asp Ile Met Val Leu Gln Glu Val Val Asp Ser Ser Gly Ser 
    50                  55                  60                  


Ala Ile Pro Leu Leu Leu Arg Glu Leu Asn Arg Phe Asp Gly Ser Gly 
65                  70                  75                  80  


Pro Tyr Ser Thr Leu Ser Ser Pro Gln Leu Gly Arg Ser Thr Tyr Met 
                85                  90                  95      


Glu Thr Tyr Val Tyr Phe Tyr Arg Ser His Lys Thr Gln Val Leu Ser 
            100                 105                 110         


Ser Tyr Val Tyr Asn Asp Glu Asp Asp Val Phe Ala Arg Glu Pro Phe 
        115                 120                 125             


Val Ala Gln Phe Ser Leu Pro Ser Asn Val Leu Pro Ser Leu Val Leu 
    130                 135                 140                 


Val Pro Leu His Thr Thr Pro Lys Ala Val Glu Lys Glu Leu Asn Ala 
145                 150                 155                 160 


Leu Tyr Asp Val Phe Leu Glu Val Ser Gln His Trp Gln Ser Lys Asp 
                165                 170                 175     


Val Ile Leu Leu Gly Asp Phe Asn Ala Asp Cys Ala Ser Leu Thr Lys 
            180                 185                 190         


Lys Arg Leu Asp Lys Leu Glu Leu Arg Thr Glu Pro Gly Phe His Trp 
        195                 200                 205             


Val Ile Ala Asp Gly Glu Asp Thr Thr Val Arg Ala Ser Thr His Cys 
    210                 215                 220                 


Thr Tyr Ala Arg Val Val Leu His Gly Glu Arg Cys Arg Ser Leu Leu 
225                 230                 235                 240 


His Thr Ala Ala Ala Phe Asp Phe Pro Thr Ser Phe Gln Leu Thr Glu 
                245                 250                 255     


Glu Glu Ala Leu Asn Ile Ser Asp His Tyr Pro Val Glu Val Glu Leu 
            260                 265                 270         


Lys Leu Ser Gln Ala His Ser Val Gln Pro Leu Ser Leu Thr Val Leu 
        275                 280                 285             


Leu Leu Leu Ser Leu Leu Ser Pro Gln Leu Cys Pro Ala Ala 
    290                 295                 300         


<210> 4
<211> 282
<212> PRT
<213> Bos Taurus

<300>
<308> P00639, UniProtKB/Swiss-Prot
<309> 1998-12-15

<400> 4
Met Arg Gly Thr Arg Leu Met Gly Leu Leu Leu Ala Leu Ala Gly Leu 
1               5                   10                  15      


Leu Gln Leu Gly Leu Ser Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr 
            20                  25                  30          


Phe Gly Glu Thr Lys Met Ser Asn Ala Thr Leu Ala Ser Tyr Ile Val 
        35                  40                  45              


Arg Ile Val Arg Arg Tyr Asp Ile Val Leu Ile Gln Glu Val Arg Asp 
    50                  55                  60                  


Ser His Leu Val Ala Val Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp 
65                  70                  75                  80  


Asp Pro Asn Thr Tyr His Tyr Val Val Ser Glu Pro Leu Gly Arg Asn 
                85                  90                  95      


Ser Tyr Lys Glu Arg Tyr Leu Phe Leu Phe Arg Pro Asn Lys Val Ser 
            100                 105                 110         


Val Leu Asp Thr Tyr Gln Tyr Asp Asp Gly Cys Glu Ser Cys Gly Asn 
        115                 120                 125             


Asp Ser Phe Ser Arg Glu Pro Ala Val Val Lys Phe Ser Ser His Ser 
    130                 135                 140                 


Thr Lys Val Lys Glu Phe Ala Ile Val Ala Leu His Ser Ala Pro Ser 
145                 150                 155                 160 


Asp Ala Val Ala Glu Ile Asn Ser Leu Tyr Asp Val Tyr Leu Asp Val 
                165                 170                 175     


Gln Gln Lys Trp His Leu Asn Asp Val Met Leu Met Gly Asp Phe Asn 
            180                 185                 190         


Ala Asp Cys Ser Tyr Val Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu 
        195                 200                 205             


Arg Thr Ser Ser Thr Phe Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr 
    210                 215                 220                 


Thr Ala Thr Ser Thr Asn Cys Ala Tyr Asp Arg Ile Val Val Ala Gly 
225                 230                 235                 240 


Ser Leu Leu Gln Ser Ser Val Val Pro Gly Ser Ala Ala Pro Phe Asp 
                245                 250                 255     


Phe Gln Ala Ala Tyr Gly Leu Ser Asn Glu Met Ala Leu Ala Ile Ser 
            260                 265                 270         


Asp His Tyr Pro Val Glu Val Thr Leu Thr 
        275                 280         


<210> 5
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with positively-charged
      basic amino acids at positions Q9 and E13
      conferring hyperactivity


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Any basic amino acid (His, Arg, Lys, Asn or Gln)

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Ser or Thr 

<220>
<221> MOD_RES
<222> (10)..(10)
<223> Any basic amino acid (His, Arg, Lys, Asn or Gln)

<400> 5
Ala Ala Phe Asn Ile Xaa Xaa Phe Gly Xaa Thr Lys Met Ser Asn 
1               5                   10                  15  


<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with positively-charged
      basic amino acid at position N74 conferring heat
      lability


<220>
<221> MOD_RES
<222> (7)..(7)
<223> Any basic amino acid (His, Arg, Lys, Asn or Gln)

<220>
<221> MOD_RES
<222> (8)..(8)
<223> Ser, Thr or Asn 

<400> 6
Ser Glu Pro Leu Gly Arg Xaa Xaa Tyr Lys Glu 
1               5                   10      


<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with Leu130 conferring heat lability


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Ala or Pro 

<400> 7
Phe Ala Leu Val Xaa Leu His 
1               5           


<210> 8
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with Ser-205 conferring heat lability


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Ala, Ser or Val

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Thr, Ser or Lys 

<220>
<221> MOD_RES
<222> (8)..(8)
<223> Ser or Pro 

<220>
<221> MOD_RES
<222> (10)..(10)
<223> Asn or His 

<400> 8
Ala Asp Thr Thr Xaa Ser Xaa Xaa Thr Xaa Cys Ala Tyr Asp Arg Ile 
1               5                   10                  15      


Val Val Ala Gly 
            20  


<210> 9
<211> 260
<212> PRT
<213> Homo sapiens

<220>
<223> human DNase I (phosphodiesterase)

<400> 9
Leu Lys Ile Ala Ala Phe Asn Ile Gln Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Val Ser Tyr Ile Val Gln Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Val Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Asn Leu Asn Gln Asp Ala Pro Asp Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Asp Gln Val Ser Ala Val Asp Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Asn Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Arg Phe Phe Ser Arg Phe Thr Glu Val Arg Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Gly Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln Glu Lys Trp Gly Leu 
145                 150                 155                 160 


Glu Asp Val Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Arg Pro Ser Gln Trp Ser Ser Ile Arg Leu Trp Thr Ser Pro Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Pro Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Met Leu Leu Arg Gly Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Leu Pro Phe Asn Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asp Gln Leu Ala Gln Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Met Leu Lys 
            260 


<210> 10
<211> 260
<212> PRT
<213> Bos taurus

<220>
<223> bovine DNase I (phosphodiesterase)

<400> 10
Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Val Arg Arg Tyr 
            20                  25                  30          


Asp Ile Val Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser His Ser Thr Lys Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Ala Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asn Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu 
145                 150                 155                 160 


Asn Asp Val Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210> 11
<211> 260
<212> PRT
<213> Ovis aries 

<220>
<223> ovine DNase I (phosphodiesterase)

<400> 11
Leu Lys Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Ser Tyr Ile Val Arg Ile Leu Arg Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Asp Leu Asn Gln Asp Asp Pro Asn Ser Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Lys Phe Ser Ser Pro Ser Thr Lys Val Lys Ala Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asn Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp Asp Leu 
145                 150                 155                 160 


Asn Asp Ile Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser 
    210                 215                 220                 


Val Val Pro Gly Ser Ala Val Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Thr 
            260 


<210> 12
<211> 260
<212> PRT
<213> Equus caballus 

<220>
<223> equine DNase I (phosphodiesterase)

<400> 12
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Asp Thr Leu Ser Asn Tyr Ile Val Gln Ile Leu Asn Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Arg Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Phe Val Val Ser Glu Pro Leu Gly Arg Asn Asn Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asp Gln Val Ser Leu Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asn Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Ser Ser Pro Phe Thr Gln Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Ser Asp Ala Leu Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp Asp Met 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Pro Ser Ile Arg Leu Arg Arg Asn Pro Ala Phe 
            180                 185                 190         


Trp Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Val Lys Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Thr Leu Leu Gln Glu Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Val Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Asn Asp Gln Thr Ala Glu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Met 
            260 


<210> 13
<211> 262
<212> PRT
<213> Canis familiaris

<220>
<223> canine DNase I (phosphodiesterase)

<400> 13
Leu Arg Met Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Lys Tyr Ile Val Gln Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Val Ala Val Val Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Thr Leu Asn Gln Asp Asp Pro Asn Ala Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Ser Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Leu Phe Arg Pro Asp Arg Val Ser Val Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Arg Phe His Ser Pro Leu Thr Glu Val Lys Glu Phe 
        115                 120                 125             


Ala Val Val Pro Leu His Ala Ala Pro Leu Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln His Lys Trp Asp Leu 
145                 150                 155                 160 


Glu Asp Ile Val Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Ala Ala Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Asn Pro Ala Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Ser Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ser Gln Leu Gln His Ala 
    210                 215                 220                 


Val Val Pro Glu Ser Ala Ala Pro Phe Asn Phe Gln Val Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Ser Gln Leu Ala Gln Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Lys Arg Ala 
            260         


<210> 14
<211> 262
<212> PRT
<213> Sus scrofa

<220>
<223> porcine DNase I (phosphodiesterase)

<400> 14
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Asn Tyr Ile Val Arg Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asn Glu Leu Asn Gln Asp Asp Pro Asn Asn Tyr His 
    50                  55                  60                  


His Val Val Ser Glu Pro Leu Gly Arg Ser Thr Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Leu 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Asn Arg Glu 
            100                 105                 110         


Pro Ser Val Val Lys Phe Ser Ser Pro Ser Thr Gln Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Ser Asp Ala Ala Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asn Val Arg Gln Lys Trp Asp Leu 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Thr Ser His Trp Ser Ser Ile Arg Leu Arg Glu Ser Pro Pro Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Val Ser Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Pro Leu Leu Gln Arg Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Phe Gly 
225                 230                 235                 240 


Leu Ser Glu Gln Thr Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Lys Arg Ala 
            260         


<210> 15
<211> 260
<212> PRT
<213> Oryctolagus cuniculus

<220>
<223> rabbit DNase I (phosphodiesterase)

<400> 15
Leu Lys Ile Ala Ala Phe Asn Ile Arg Ser Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Thr Ser Tyr Ile Val Arg Ile Leu Gln Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Lys Leu Asn Glu Lys Ala Ala Asp Thr Tyr Arg 
    50                  55                  60                  


Phe Val Ala Ser Glu Pro Leu Gly Arg Arg Thr Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Thr Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Val Val Arg Phe Ser Ser Pro Ser Thr Lys Val Arg Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ser Ala Pro Glu Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln Lys Lys Trp Gly Leu 
145                 150                 155                 160 


Gln Asp Val Met Leu Met Gly Asp Phe Asn Ala Asp Tyr Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Asn Pro Ala Phe 
            180                 185                 190         


Lys Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Ala Thr Ser Thr Asn 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Pro Leu Leu Gln Asp Ala 
    210                 215                 220                 


Val Val Pro Asn Ser Ala Ala Pro Phe Asn Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Gln Leu Ala Gln Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Ala 
            260 


<210> 16
<211> 262
<212> PRT
<213> Mus musculus

<220>
<223> mouse DNase I (phosphodiesterase)

<400> 16
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Val Tyr Phe Val Lys Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Val Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Glu Leu Asn Arg Asp Lys Pro Asp Thr Tyr Arg 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Lys Ser Tyr Lys Glu Gln Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Asp Gln Val Ser Ile Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Phe Ser Pro Tyr Thr Glu Val Gln Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Thr Glu Ala Val Ser Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Trp Gln Lys Trp Gly Leu 
145                 150                 155                 160 


Glu Asp Ile Met Phe Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Ile Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ala Leu Leu Gln Ala Ala 
    210                 215                 220                 


Val Val Pro Asn Ser Ala Val Pro Phe Asp Phe Gln Ala Glu Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Gln Leu Ala Glu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Arg Lys Ile 
            260         


<210> 17
<211> 262
<212> PRT
<213> Rattus norvegicus

<220>
<223> rat DNase I (phosphodiesterase)

<400> 17
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Asp Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Ser Tyr Ile Val Lys Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Val Val Gln Glu Val Arg Asp Thr His Leu Val Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Glu Leu Asn Arg Asp Ile Pro Asp Asn Tyr Arg 
    50                  55                  60                  


Tyr Ile Ile Ser Glu Pro Leu Gly Arg Lys Ser Tyr Lys Glu Gln Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Pro Ser Gln Val Ser Val Leu Asp Ser Tyr His 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Phe Ser Pro Tyr Thr Glu Val Arg Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ser Ala Pro Thr Glu Ala Val Ser Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Arg Gln Lys Trp Gly Leu 
145                 150                 155                 160 


Glu Asp Ile Met Phe Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Ile Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Ala Leu Leu Gln Ala Ala 
    210                 215                 220                 


Val Val Pro Ser Ser Ala Val Pro Phe Asp Phe Gln Ala Glu Tyr Arg 
225                 230                 235                 240 


Leu Thr Asn Gln Met Ala Glu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Arg Lys Thr 
            260         


<210> 18
<211> 262
<212> PRT
<213> Gallus gallus

<220>
<223> chicken DNase I (phosphodiesterase)

<400> 18
Leu Arg Ile Ser Ala Phe Asn Ile Arg Thr Phe Gly Asp Ser Lys Met 
1               5                   10                  15      


Ser Asn Gln Thr Val Ala Gly Phe Ile Val Ser Ile Leu Val Gln Tyr 
            20                  25                  30          


Asp Ile Thr Leu Val Gln Glu Val Arg Asp Ala Asp Leu Ser Ser Val 
        35                  40                  45              


Lys Lys Leu Val Ser Gln Leu Asn Ser Ala Ser Ser Tyr Pro Tyr Ser 
    50                  55                  60                  


Phe Leu Ser Ser Ile Pro Leu Gly Arg Asn Ser Tyr Lys Glu Gln Tyr 
65                  70                  75                  80  


Val Phe Ile Tyr Arg Ser Asp Ile Val Ser Val Leu Glu Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Ser Cys Gly Thr Asp Ile Phe Ser Arg Glu 
            100                 105                 110         


Pro Phe Ile Val Lys Phe Ser Ser Pro Thr Thr Gln Leu Asp Glu Phe 
        115                 120                 125             


Val Ile Val Pro Leu His Ala Glu Pro Ser Ser Ala Pro Ala Glu Ile 
    130                 135                 140                 


Asn Ala Leu Thr Asp Val Tyr Thr Asp Val Ile Asn Lys Trp Glu Thr 
145                 150                 155                 160 


Asn Asn Ile Phe Phe Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val 
                165                 170                 175     


Thr Ala Glu Gln Trp Pro Ser Ile Arg Leu Arg Ser Leu Ser Ser Cys 
            180                 185                 190         


Glu Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Thr Ser Thr Asp 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Ala Cys Gly Ser Ala Leu Arg Gln Ala 
    210                 215                 220                 


Val Glu Tyr Gly Ser Ala Thr Val Asn Asn Phe Gln Glu Thr Leu Arg 
225                 230                 235                 240 


Ile Gln Asn Lys Asp Ala Leu Ala Ile Ser Asp His Phe Pro Val Glu 
                245                 250                 255     


Val Thr Leu Lys Ala Arg 
            260         


<210> 19
<211> 262
<212> PRT
<213> Elaphe quadrivirgata

<220>
<223> snake DNase I (phosphodiesterase)

<400> 19
Leu Arg Ile Gly Ala Phe Asn Ile Arg Ala Phe Gly Asp Lys Lys Leu 
1               5                   10                  15      


Ser Asn Gln Thr Ile Ser Ser Ser Ile Val Arg Ile Leu Thr Thr Tyr 
            20                  25                  30          


Asp Leu Val Leu Ile Gln Glu Val Arg Asp Ala Asp Leu Ser Ala Val 
        35                  40                  45              


Lys Lys Leu Met Gln Leu Val Ser Gly Ala Ser Pro Asp Pro Phe Gly 
    50                  55                  60                  


Tyr Leu Ile Ser Lys Pro Leu Gly His Asn Ser Tyr Lys Glu Gln Tyr 
65                  70                  75                  80  


Leu Phe Val Tyr Arg Gln Asp Arg Val Ser Pro Val Glu Ser Tyr Tyr 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Gly Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Phe Ile Val Lys Phe Ala Val Pro Gln Ala Ala Val Glu Glu Leu 
        115                 120                 125             


Val Leu Val Pro Leu His Ala Ala Pro Glu Ala Ala Val Thr Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Gln Asp Val Lys Asp Arg Trp Gly Val 
145                 150                 155                 160 


Thr Asp Ala Leu Leu Leu Gly Asp Phe Asn Ala Asp Cys Asn Tyr Val 
                165                 170                 175     


Gln Ala Glu Asp Trp Pro Ser Ile Arg Leu Arg Ser Ser Lys Asp Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Val Thr Asn Thr Ile 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Ala Val Gly Ser Lys Leu Arg Glu Ser 
    210                 215                 220                 


Ile Leu Pro Ala Thr Ala Lys Val Asp Asn Phe Gln Lys Thr Leu Lys 
225                 230                 235                 240 


Leu Ser Ser Lys Asp Ala Leu Ala Val Ser Asp His Phe Pro Val Glu 
                245                 250                 255     


Val Thr Leu Lys Ser Thr 
            260         


<210> 20
<211> 331
<212> PRT
<213> Xenopus laevis

<220>
<223> African clawed frog DNase I (phosphodiesterase)

<400> 20
Phe Lys Ile Ala Ser Phe Asn Ile Gln Arg Phe Ser Met Thr Lys Val 
1               5                   10                  15      


Asp Asp Pro Val Val Leu Glu Leu Leu Ile Arg Ile Leu Ser Arg Tyr 
            20                  25                  30          


Glu Ile Ile Ala Ile Glu Glu Val Met Asn Ala Asp Asn Thr Ala Ile 
        35                  40                  45              


Ile Ser Leu Val Lys Glu Leu Ser Leu Ala Thr Lys Leu Asn Tyr Asn 
    50                  55                  60                  


Val Leu Ile Ser Asp His Leu Gly Arg Ser Ser Tyr Arg Glu Lys Tyr 
65                  70                  75                  80  


Ala Tyr Val Tyr Arg Glu Asp Ile Val Lys Pro Thr Glu Trp Tyr His 
                85                  90                  95      


Phe Asp Asp Gly Cys Glu Asn Cys Gly Thr Asp Ser Phe Ile Arg Glu 
            100                 105                 110         


Pro Phe Val Ala Arg Phe Thr Ser Leu Thr Thr Val Val Lys Asp Phe 
        115                 120                 125             


Ala Leu Ile Ser Ile His Thr Ser Pro Asp Tyr Ala Ile Met Glu Val 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Ala Trp Val Asp Ala Lys Gln Arg Leu Lys Met 
145                 150                 155                 160 


Glu Asn Ile Leu Ile Leu Gly Asp Tyr Asn Ala Ala Cys Ser Tyr Val 
                165                 170                 175     


Ala Ser Arg His Trp Pro Ile Ile Arg Leu Arg His Val Glu Glu Leu 
            180                 185                 190         


Val Trp Leu Ile Gly Asp Lys Glu Asp Thr Thr Val Ser Thr Asn Thr 
        195                 200                 205             


Asn Cys Ala Tyr Asp Arg Met Val Ala Gly Gly Glu Glu Leu Gln Arg 
    210                 215                 220                 


Gly Ile Val Pro Asp Thr Ala Lys Ala Phe Asn Tyr His Val Ala Tyr 
225                 230                 235                 240 


Asp Leu Thr Tyr Glu Met Ala Lys Ala Val Ser Asp His Tyr Pro Val 
                245                 250                 255     


Glu Val Glu Leu Tyr Asp Asp Val Phe Tyr Ser Gly Gln Cys Phe Glu 
            260                 265                 270         


Pro Ser Ala Ser Thr Gly Ile Ser Gly Gly Leu Ser Leu Asn Gly Pro 
        275                 280                 285             


Cys Thr Cys Glu Gly Val Asp Phe Ser Ser Cys Arg Gly Arg Cys Gly 
    290                 295                 300                 


Ala Ser Gly Lys Thr Tyr Pro Cys Asn Cys Asn Ala Ser Cys Thr Asn 
305                 310                 315                 320 


Cys Cys Val Asp Tyr Thr Ser Ser Cys Lys Leu 
                325                 330     


<210> 21
<211> 262
<212> PRT
<213> Anguilla japonica

<220>
<223> Japanese eel DNase I (phosphodiesterase)

<400> 21
Leu Phe Ile Gly Ala Phe Asn Ile Arg Ser Phe Gly Asp Lys Lys Ala 
1               5                   10                  15      


Ser Asn Ala Thr Leu Val Asp Ile Ile Val Lys Ile Val His Met Tyr 
            20                  25                  30          


Asp Ile Leu Leu Ile Gln Glu Val Arg Asp Ser Asp Leu Ser Ala Thr 
        35                  40                  45              


Lys Lys Leu Met Gln Asn Val Asn Gly Gly Ser Ser Pro His Lys Tyr 
    50                  55                  60                  


Lys Tyr Ile Val Ser Glu Pro Leu Gly Arg Asn Thr Tyr Gln Glu Arg 
65                  70                  75                  80  


Tyr Leu Tyr Leu Tyr Arg Glu Asp Ser Val Ser Val Val Lys Asn Phe 
                85                  90                  95      


Thr Tyr Asp Asp Gly Ala Glu Ala Ser Gly Thr Asp Thr Phe Asn Arg 
            100                 105                 110         


Glu Pro Phe Val Val Met Phe Ser Ser Pro His Thr Arg Val Pro Glu 
        115                 120                 125             


Phe Ala Leu Val Pro Gln His Thr Ser Pro Asp Glu Ala Val Lys Glu 
    130                 135                 140                 


Ile Asp Ala Leu Tyr Asp Val Ile Val Asp Ile Arg Ala Arg Trp Asn 
145                 150                 155                 160 


Thr Asp Asn Ile Ile Leu Leu Gly Asp Phe Asn Ala Gly Cys Asn Tyr 
                165                 170                 175     


Val Ala Gly Ser Asp Trp Gln Gln Ile Arg Leu Tyr Thr Asp Lys Ser 
            180                 185                 190         


Phe His Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Val Ser His Thr 
        195                 200                 205             


Asn Cys Pro Tyr Asp Arg Ile Val Ala Thr Thr Thr Met Met Glu Ala 
    210                 215                 220                 


Val Val Pro His Ser Ala Ser Val Tyr Asp Tyr Met Thr Ser Leu Lys 
225                 230                 235                 240 


Leu Lys Leu Asp Met Ala Leu Ala Val Ser Asp His Phe Pro Val Glu 
                245                 250                 255     


Val Gln Leu Phe Gly Pro 
            260         


<210> 22
<211> 261
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      equine DNase I "KS" mutant having two
      hyperactive (R13, K74) and two heat-labile (L130,
      S-205 insertion) mutations

<400> 22
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Arg Thr Lys Met 
1               5                   10                  15      


Ser Asn Asp Thr Leu Ser Asn Tyr Ile Val Gln Ile Leu Asn Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Arg Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Phe Val Val Ser Glu Pro Leu Gly Arg Lys Asn Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asp Gln Val Ser Leu Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asn Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Ser Ser Pro Phe Thr Gln Val Lys Glu Phe 
        115                 120                 125             


Ala Leu Val Pro Leu His Ala Ala Pro Ser Asp Ala Leu Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp Asp Met 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Pro Ser Ile Arg Leu Arg Arg Asn Pro Ala Phe 
            180                 185                 190         


Trp Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Val Ser Lys Ser Thr 
        195                 200                 205             


His Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Thr Leu Leu Gln Glu 
    210                 215                 220                 


Ala Val Val Pro Asp Ser Ala Val Pro Phe Asp Phe Gln Ala Ala Tyr 
225                 230                 235                 240 


Gly Leu Asn Asp Gln Thr Ala Glu Ala Ile Ser Asp His Tyr Pro Val 
                245                 250                 255     


Glu Val Thr Leu Met 
            260     


<210> 23
<211> 260
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      equine DNase I "K" mutant having two
      hyperactive (R13, K74) and one heat-labile (L130)
      mutation

<400> 23
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Arg Thr Lys Met 
1               5                   10                  15      


Ser Asn Asp Thr Leu Ser Asn Tyr Ile Val Gln Ile Leu Asn Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Arg Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Phe Val Val Ser Glu Pro Leu Gly Arg Lys Asn Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asp Gln Val Ser Leu Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asn Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Ser Ser Pro Phe Thr Gln Val Lys Glu Phe 
        115                 120                 125             


Ala Leu Val Pro Leu His Ala Ala Pro Ser Asp Ala Leu Ala Glu Ile 
    130                 135                 140                 


Asp Ser Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp Asp Met 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Pro Ser Ile Arg Leu Arg Arg Asn Pro Ala Phe 
            180                 185                 190         


Trp Trp Leu Ile Pro Asp Thr Ala Asp Thr Thr Val Lys Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Thr Leu Leu Gln Glu Ala 
    210                 215                 220                 


Val Val Pro Asp Ser Ala Val Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Asn Asp Gln Thr Ala Glu Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Met 
            260 


<210> 24
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with positively-charged basic amino acids
      at positions Q9 and E13 conferring hyperactivity

<220>
<221> MOD_RES
<222> (7)..(7)
<223> Ser or Thr

<400> 24
Ala Ala Phe Asn Ile Arg Xaa Phe Gly Arg Thr Lys Met Ser Asn 
1               5                   10                  15  


<210> 25
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I motif with positively-charged basic amino acid
      at position N74 conferring heat lability


<220>
<221> MOD_RES
<222> (8)..(8)
<223> Ser, Thr or Asn

<400> 25
Ser Glu Pro Leu Gly Arg Lys Xaa Tyr Lys Glu 
1               5                   10      


<210> 26
<211> 262
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      DNase I consensus sequence


<220>
<221> MOD_RES
<222> (27)..(27)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (54)..(54)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (74)..(74)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (122)..(122)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (124)..(124)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (138)..(138)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (159)..(159)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (191)..(191)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (219)..(219)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (223)..(223)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (228)..(228)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (231)..(231)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (245)..(245)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (247)..(247)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (260)..(260)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (261)..(261)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (262)..(262)
<223> Any amino acid

<400> 26
Leu Arg Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met 
1               5                   10                  15      


Ser Asn Ala Thr Leu Ser Ser Tyr Ile Val Xaa Ile Leu Ser Arg Tyr 
            20                  25                  30          


Asp Ile Ala Leu Ile Gln Glu Val Arg Asp Ser His Leu Thr Ala Val 
        35                  40                  45              


Gly Lys Leu Leu Asp Xaa Leu Asn Gln Asp Asp Pro Asn Thr Tyr His 
    50                  55                  60                  


Tyr Val Val Ser Glu Pro Leu Gly Arg Xaa Ser Tyr Lys Glu Arg Tyr 
65                  70                  75                  80  


Leu Phe Val Phe Arg Pro Asp Gln Val Ser Val Leu Asp Ser Tyr Gln 
                85                  90                  95      


Tyr Asp Asp Gly Cys Glu Pro Cys Gly Asn Asp Thr Phe Ser Arg Glu 
            100                 105                 110         


Pro Ala Ile Val Lys Phe Ser Ser Pro Xaa Thr Xaa Val Lys Glu Phe 
        115                 120                 125             


Ala Ile Val Pro Leu His Ala Ala Pro Xaa Asp Ala Val Ala Glu Ile 
    130                 135                 140                 


Asp Ala Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp Xaa Leu 
145                 150                 155                 160 


Glu Asp Ile Met Leu Met Gly Asp Phe Asn Ala Gly Cys Ser Tyr Val 
                165                 170                 175     


Thr Ser Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Pro Xaa Phe 
            180                 185                 190         


Gln Trp Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr His 
        195                 200                 205             


Cys Ala Tyr Asp Arg Ile Val Val Ala Gly Xaa Leu Leu Gln Xaa Ala 
    210                 215                 220                 


Val Val Pro Xaa Ser Ala Xaa Pro Phe Asp Phe Gln Ala Ala Tyr Gly 
225                 230                 235                 240 


Leu Ser Asn Gln Xaa Ala Xaa Ala Ile Ser Asp His Tyr Pro Val Glu 
                245                 250                 255     


Val Thr Leu Xaa Xaa Xaa 
            260         


<210> 27
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 27
His His His His His His 
1               5       


<210> 28
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      Peptide


<220>
<221> MISC_FEATURE
<222> (1)...(20)
<223> This sequence may encompass 1-10 Gly Serrepeating units

<400> 28
Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10                  15      


Gly Ser Gly Ser 
            20  


<210> 29
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MISC_FEATURE
<222> (1)...(20)
<223> This sequence may encompass 1-10 Gly Gly Ser repeating 
      units

<400> 29
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            20                  25                  30  


<210> 30
<211> 40
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MISC_FEATURE
<222> (1)...(20)
<223> This sequence may encompass 1-10 Gly Gly Gly Ser 
      repeating units

<400> 30
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 
            20                  25                  30          


Gly Gly Gly Ser Gly Gly Gly Ser 
        35                  40  


