                         SEQUENCE LISTING

<110>  Technische Universitt Dresden
       Universitt Leipzig
 
<120>  Verfahren zum Nachweis von Analyten in Proben

<130>  00017P0361DEWO

<150>  DE102018130134.0
<151>  2018-11-28

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  1284
<212>  DNA
<213>  Escherichia coli

<400>  1
atggaatccc tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt       60

tccaagagcg tttctaaccg cgctttattg ctggcggcat tagcacacgg caaaacagta      120

ttaaccaatc tgctggatag cgatgacgtg cgccatatgc tgaatgcatt aacagcgtta      180

ggggtaagct atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt      240

ccattacacg cagaaggtgc cctggagttg ttcctcggta acgccggaac ggcaatgcgt      300

ccgctggcgg cagctctttg tctgggtagc aatgatattg tgctgaccgg tgagccgcgt      360

atgaaagaac gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc      420

acttacctgg aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc      480

aacgttgacg ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg      540

cctcttgcgc cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat      600

atcgacatca cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat      660

caacaatttg tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa      720

ggcgatgcat cttcggcttc ttactttctg gcagcagcag caatcaaagg cggcactgta      780

aaagtgaccg gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg      840

gaaaaaatgg gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa      900

ctgaacgcta ttgatatgga tatgaaccat attcctgatg cggcgatgac cattgccacg      960

gcggcgttat ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa     1020

gagaccgatc gcctgtttgc gatggcaaca gaactgcgta aagtcggcgc ggaagtggaa     1080

gaggggcacg attacattcg tatcactcct ccggaaaaac tgaactttgc cgagatcgcg     1140

acatacaatg atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca     1200

gtgacgattc ttgatcccaa atgcacggcc aaaacatttc cggattattt cgagcagctg     1260

gcgcggatta gccaggcagc ctaa                                            1284


<210>  2
<211>  240
<212>  DNA
<213>  Neurospora crassa

<400>  2
atcggcccca acacctgctc catcgacgac tacaagcctt actgctgcca gtctatgtcc       60

ggccccgccg gctcccctgg tctcctcaac ctcatccccg tcgacctcag cgcctcgctc      120

ggctgcgttg tcggtgtcat cggctcccaa tgtggtgcca gcgtcaagtg ctgcaaggac      180

gatgttacca acaccggcaa ctccttcctc atcatcaacg ctgccaactg cgttgcctaa      240


<210>  3
<211>  1572
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fusions protein of EPSPS/Ccg2

<400>  3
atcggcccca acacctgctc catcgacgac tacaagcctt actgctgcca gtctatgtcc       60

ggccccgccg gctcccctgg tctcctcaac ctcatccccg tcgacctcag cgcctcgctc      120

ggctgcgttg tcggtgtcat cggctcccaa tgtggtgcca gcgtcaagtg ctgcaaggac      180

gatgttacca acaccggcaa ctccttcctc atcatcaacg ctgccaactg cgttgccgaa      240

ttcggcggcg gcggcagcgg cggcggcggc agcggcggcg gcggcagcat ggaatccctg      300

acgttacaac ccatcgctcg tgtcgatggc actattaatc tgcccggttc caagagcgtt      360

tctaaccgcg ctttattgct ggcggcatta gcacacggca aaacagtatt aaccaatctg      420

ctggatagcg atgacgtgcg ccatatgctg aatgcattaa cagcgttagg ggtaagctat      480

acgctttcag ccgatcgtac gcgttgcgaa attatcggta acggcggtcc attacacgca      540

gaaggtgccc tggagttgtt cctcggtaac gccggaacgg caatgcgtcc gctggcggca      600

gctctttgtc tgggtagcaa tgatattgtg ctgaccggtg agccgcgtat gaaagaacgc      660

ccgattggtc atctggtgga tgcgctgcgc ctgggcgggg cgaagatcac ttacctggaa      720

caagaaaatt atccgccgtt gcgtttacag ggcggcttta ctggcggcaa cgttgacgtt      780

gatggctccg tttccagcca attcctcacc gcactgttaa tgactgcgcc tcttgcgccg      840

gaagatacgg tgattcgtat taaaggcgat ctggtttcta aaccttatat cgacatcaca      900

ctcaatctga tgaagacgtt tggtgttgaa attgaaaatc agcactatca acaatttgtc      960

gtaaaaggcg ggcagtctta tcagtctccg ggtacttatt tggtcgaagg cgatgcatct     1020

tcggcttctt actttctggc agcagcagca atcaaaggcg gcactgtaaa agtgaccggt     1080

attggacgta acagtatgca gggtgatatt cgctttgctg atgtgctgga aaaaatgggc     1140

gcgaccattt gctggggcga tgattatatt tcctgcacgc gtggtgaact gaacgctatt     1200

gatatggata tgaaccatat tcctgatgcg gcgatgacca ttgccacggc ggcgttattt     1260

gcaaaaggca ccaccacgct gcgcaatatc tataactggc gtgttaaaga gaccgatcgc     1320

ctgtttgcga tggcaacaga actgcgtaaa gtcggcgcgg aagtggaaga ggggcacgat     1380

tacattcgta tcactcctcc ggaaaaactg aactttgccg agatcgcgac atacaatgat     1440

caccggatgg cgatgtgttt ctcgctggtg gcgttgtcag atacaccagt gacgattctt     1500

gatcccaaat gcacggccaa aacatttccg gattatttcg agcagctggc gcggattagc     1560

caggcagcct aa                                                         1572


<210>  4
<211>  427
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Glu Ser Leu Thr Leu Gln Pro Ile Ala Arg Val Asp Gly Thr Ile 
1               5                   10                  15      


Asn Leu Pro Gly Ser Lys Ser Val Ser Asn Arg Ala Leu Leu Leu Ala 
            20                  25                  30          


Ala Leu Ala His Gly Lys Thr Val Leu Thr Asn Leu Leu Asp Ser Asp 
        35                  40                  45              


Asp Val Arg His Met Leu Asn Ala Leu Thr Ala Leu Gly Val Ser Tyr 
    50                  55                  60                  


Thr Leu Ser Ala Asp Arg Thr Arg Cys Glu Ile Ile Gly Asn Gly Gly 
65                  70                  75                  80  


Pro Leu His Ala Glu Gly Ala Leu Glu Leu Phe Leu Gly Asn Ala Gly 
                85                  90                  95      


Thr Ala Met Arg Pro Leu Ala Ala Ala Leu Cys Leu Gly Ser Asn Asp 
            100                 105                 110         


Ile Val Leu Thr Gly Glu Pro Arg Met Lys Glu Arg Pro Ile Gly His 
        115                 120                 125             


Leu Val Asp Ala Leu Arg Leu Gly Gly Ala Lys Ile Thr Tyr Leu Glu 
    130                 135                 140                 


Gln Glu Asn Tyr Pro Pro Leu Arg Leu Gln Gly Gly Phe Thr Gly Gly 
145                 150                 155                 160 


Asn Val Asp Val Asp Gly Ser Val Ser Ser Gln Phe Leu Thr Ala Leu 
                165                 170                 175     


Leu Met Thr Ala Pro Leu Ala Pro Glu Asp Thr Val Ile Arg Ile Lys 
            180                 185                 190         


Gly Asp Leu Val Ser Lys Pro Tyr Ile Asp Ile Thr Leu Asn Leu Met 
        195                 200                 205             


Lys Thr Phe Gly Val Glu Ile Glu Asn Gln His Tyr Gln Gln Phe Val 
    210                 215                 220                 


Val Lys Gly Gly Gln Ser Tyr Gln Ser Pro Gly Thr Tyr Leu Val Glu 
225                 230                 235                 240 


Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Ala Ala Ala Ile Lys 
                245                 250                 255     


Gly Gly Thr Val Lys Val Thr Gly Ile Gly Arg Asn Ser Met Gln Gly 
            260                 265                 270         


Asp Ile Arg Phe Ala Asp Val Leu Glu Lys Met Gly Ala Thr Ile Cys 
        275                 280                 285             


Trp Gly Asp Asp Tyr Ile Ser Cys Thr Arg Gly Glu Leu Asn Ala Ile 
    290                 295                 300                 


Asp Met Asp Met Asn His Ile Pro Asp Ala Ala Met Thr Ile Ala Thr 
305                 310                 315                 320 


Ala Ala Leu Phe Ala Lys Gly Thr Thr Thr Leu Arg Asn Ile Tyr Asn 
                325                 330                 335     


Trp Arg Val Lys Glu Thr Asp Arg Leu Phe Ala Met Ala Thr Glu Leu 
            340                 345                 350         


Arg Lys Val Gly Ala Glu Val Glu Glu Gly His Asp Tyr Ile Arg Ile 
        355                 360                 365             


Thr Pro Pro Glu Lys Leu Asn Phe Ala Glu Ile Ala Thr Tyr Asn Asp 
    370                 375                 380                 


His Arg Met Ala Met Cys Phe Ser Leu Val Ala Leu Ser Asp Thr Pro 
385                 390                 395                 400 


Val Thr Ile Leu Asp Pro Lys Cys Thr Ala Lys Thr Phe Pro Asp Tyr 
                405                 410                 415     


Phe Glu Gln Leu Ala Arg Ile Ser Gln Ala Ala 
            420                 425         


<210>  5
<211>  79
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CCg2 of Neurospora crassa optimized

<400>  5

Ile Gly Pro Asn Thr Cys Ser Ile Asp Asp Tyr Lys Pro Tyr Cys Cys 
1               5                   10                  15      


Gln Ser Met Ser Gly Pro Ala Gly Ser Pro Gly Leu Leu Asn Leu Ile 
            20                  25                  30          


Pro Val Asp Leu Ser Ala Ser Leu Gly Cys Val Val Gly Val Ile Gly 
        35                  40                  45              


Ser Gln Cys Gly Ala Ser Val Lys Cys Cys Lys Asp Asp Val Thr Asn 
    50                  55                  60                  


Thr Gly Asn Ser Phe Leu Ile Ile Asn Ala Ala Asn Cys Val Ala 
65                  70                  75                  


<210>  6
<211>  523
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusionsprotein EPSPS/Ccg2

<400>  6

Ile Gly Pro Asn Thr Cys Ser Ile Asp Asp Tyr Lys Pro Tyr Cys Cys 
1               5                   10                  15      


Gln Ser Met Ser Gly Pro Ala Gly Ser Pro Gly Leu Leu Asn Leu Ile 
            20                  25                  30          


Pro Val Asp Leu Ser Ala Ser Leu Gly Cys Val Val Gly Val Ile Gly 
        35                  40                  45              


Ser Gln Cys Gly Ala Ser Val Lys Cys Cys Lys Asp Asp Val Thr Asn 
    50                  55                  60                  


Thr Gly Asn Ser Phe Leu Ile Ile Asn Ala Ala Asn Cys Val Ala Glu 
65                  70                  75                  80  


Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                85                  90                  95      


Met Glu Ser Leu Thr Leu Gln Pro Ile Ala Arg Val Asp Gly Thr Ile 
            100                 105                 110         


Asn Leu Pro Gly Ser Lys Ser Val Ser Asn Arg Ala Leu Leu Leu Ala 
        115                 120                 125             


Ala Leu Ala His Gly Lys Thr Val Leu Thr Asn Leu Leu Asp Ser Asp 
    130                 135                 140                 


Asp Val Arg His Met Leu Asn Ala Leu Thr Ala Leu Gly Val Ser Tyr 
145                 150                 155                 160 


Thr Leu Ser Ala Asp Arg Thr Arg Cys Glu Ile Ile Gly Asn Gly Gly 
                165                 170                 175     


Pro Leu His Ala Glu Gly Ala Leu Glu Leu Phe Leu Gly Asn Ala Gly 
            180                 185                 190         


Thr Ala Met Arg Pro Leu Ala Ala Ala Leu Cys Leu Gly Ser Asn Asp 
        195                 200                 205             


Ile Val Leu Thr Gly Glu Pro Arg Met Lys Glu Arg Pro Ile Gly His 
    210                 215                 220                 


Leu Val Asp Ala Leu Arg Leu Gly Gly Ala Lys Ile Thr Tyr Leu Glu 
225                 230                 235                 240 


Gln Glu Asn Tyr Pro Pro Leu Arg Leu Gln Gly Gly Phe Thr Gly Gly 
                245                 250                 255     


Asn Val Asp Val Asp Gly Ser Val Ser Ser Gln Phe Leu Thr Ala Leu 
            260                 265                 270         


Leu Met Thr Ala Pro Leu Ala Pro Glu Asp Thr Val Ile Arg Ile Lys 
        275                 280                 285             


Gly Asp Leu Val Ser Lys Pro Tyr Ile Asp Ile Thr Leu Asn Leu Met 
    290                 295                 300                 


Lys Thr Phe Gly Val Glu Ile Glu Asn Gln His Tyr Gln Gln Phe Val 
305                 310                 315                 320 


Val Lys Gly Gly Gln Ser Tyr Gln Ser Pro Gly Thr Tyr Leu Val Glu 
                325                 330                 335     


Gly Asp Ala Ser Ser Ala Ser Tyr Phe Leu Ala Ala Ala Ala Ile Lys 
            340                 345                 350         


Gly Gly Thr Val Lys Val Thr Gly Ile Gly Arg Asn Ser Met Gln Gly 
        355                 360                 365             


Asp Ile Arg Phe Ala Asp Val Leu Glu Lys Met Gly Ala Thr Ile Cys 
    370                 375                 380                 


Trp Gly Asp Asp Tyr Ile Ser Cys Thr Arg Gly Glu Leu Asn Ala Ile 
385                 390                 395                 400 


Asp Met Asp Met Asn His Ile Pro Asp Ala Ala Met Thr Ile Ala Thr 
                405                 410                 415     


Ala Ala Leu Phe Ala Lys Gly Thr Thr Thr Leu Arg Asn Ile Tyr Asn 
            420                 425                 430         


Trp Arg Val Lys Glu Thr Asp Arg Leu Phe Ala Met Ala Thr Glu Leu 
        435                 440                 445             


Arg Lys Val Gly Ala Glu Val Glu Glu Gly His Asp Tyr Ile Arg Ile 
    450                 455                 460                 


Thr Pro Pro Glu Lys Leu Asn Phe Ala Glu Ile Ala Thr Tyr Asn Asp 
465                 470                 475                 480 


His Arg Met Ala Met Cys Phe Ser Leu Val Ala Leu Ser Asp Thr Pro 
                485                 490                 495     


Val Thr Ile Leu Asp Pro Lys Cys Thr Ala Lys Thr Phe Pro Asp Tyr 
            500                 505                 510         


Phe Glu Gln Leu Ala Arg Ile Ser Gln Ala Ala 
        515                 520             


