                         SEQUENCE LISTING

<110>  WILLIAM MARSH RICE UNIVERSITY
 
<120>  BACILLUS EXPRESSION SYSTEM

<130>  RICE:2019-029-02WO

<150>  62/776,132
<151>  2018-12-06

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  154
<212>  DNA
<213>  Escherichia coli

<400>  1
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaagctgtca       60

ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt      120

tgtttaaaag cttacataag gaggaactac tatg                                  154


<210>  2
<211>  109
<212>  DNA
<213>  Escherichia coli

<400>  2
ggtaaatgtg agcactcaca attcattttg caaaagttgt tgactttatc tacaaggtgt       60

ggcataatgt gtgtaattgt gagcggataa caattaagct tagtcgaca                  109


<210>  3
<211>  883
<212>  PRT
<213>  T7 PHAGE

<400>  3

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             


Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 


Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         


Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
305                 310                 315                 320 


Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
                325                 330                 335     


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
        355                 360                 365             


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 


Ala Phe Ala 
            


<210>  4
<211>  94
<212>  DNA
<213>  Bacillus licheniformis

<400>  4
cggtggaaac gaggtcatca tttccttccg aaaaaacggt tgcatttaaa tcttacatat       60

gtaatacttt caaagactac atttgtaaga tttg                                   94


<210>  5
<211>  5152
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacI-T7-sfgfp

<400>  5
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaagcttac       60

ataaggagga actactatgc gtaaaggcga agagctgttc actggtgtcg tccctattct      120

ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg      180

tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt      240

accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc      300

ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga      360

acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga      420

aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa      480

tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga      540

taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag      600

cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct      660

gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg      720

cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga      780

actgtacaaa tgatgataat aatctagacc aggcatcaaa taaaacgaaa ggctcagtcg      840

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac      900

actggctcac cttcgggtgg gcctttctgc gtttatacgt ttcggtgatg aagatcttcc      960

cgatgattaa ttaattcaga acgctcggtt gccgccgggc gttttttatg cagcaatggc     1020

aagaacgttg ctcgagggta aatgtgagca ctcacaattc attttgcaaa agttgttgac     1080

tttatctaca aggtgtggca taatgtgtgt aattgtgagc ggataacaat taagcttagt     1140

cgacagctag cacataagga ggaactacta tgaacacgat taacatcgct aagaacgact     1200

tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc     1260

gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct     1320

tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca     1380

agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg     1440

aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc     1500

cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca     1560

atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct     1620

tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca     1680

acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc     1740

tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc     1800

atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc     1860

gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg     1920

ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt     1980

gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc     2040

gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg     2100

tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca     2160

acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg     2220

acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc     2280

ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc     2340

gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc     2400

ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa     2460

tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac     2520

caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg     2580

ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg     2640

cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct     2700

tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct     2760

cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc     2820

gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct     2880

acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg     2940

ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc     3000

tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta     3060

agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc     3120

tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga     3180

atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag     3240

ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca     3300

aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg     3360

atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt     3420

tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg     3480

cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc     3540

ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc     3600

acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa     3660

ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg     3720

accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga     3780

acctccgtga catcttagag tcggacttcg cgttcgcgta agcatgcaag ctaattcggt     3840

ggaaacgagg tcatcatttc cttccgaaaa aacggttgca tttaaatctt acatatgtaa     3900

tactttcaaa gactacattt gtaagatttg atgtttgagt cggctgaaag atcgtacgta     3960

ccaattattg tttcgtgatt gttcaagcca taacactgta gggatagtgg aaagagtgct     4020

tcatctggtt acgatcaatc aaatattcaa acggagggag acgattttga tgaaaccagt     4080

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     4140

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     4200

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     4260

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     4320

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     4380

cgaagcctgt aaaacggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     4440

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     4500

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     4560

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     4620

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     4680

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     4740

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     4800

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     4860

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     4920

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     4980

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     5040

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     5100

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt ga             5152


<210>  6
<211>  10641
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARTIFICIAL VECTOR

<400>  6
gttcagctca gtgatacctg cgatccctcc gcgattcacg acagccggca caccgatgta       60

cacgtcatct gcaccgtatt gcccgtccaa atatgtgctg acagttaata tgctgttttc      120

attatgaaga atggcttttg taatgcgagc aagactcatc gcaaccccat aataagtcgc      180

gccttttttc tcaatgatat ggtaagctgc gtttttcaca tcatctacaa tttggtccag      240

ctcctcttgt ttgtacgcat cgtttttctc aacgagttca ctgaccggca caccgccgac      300

attcgcgtgg ctccaaacag gaagctctgt gtcgccgtgc tctccgataa tatgcgcgtg      360

tacgttttga ggcgctgcgc caaagtattc gctcagcatg aaacggaatc tcgcagaatc      420

aagtgttgtg ccgcttccaa tcacccgctc ttttggcagg ccgctgaatt tccatgttgc      480

gtaagtcagg atatcaaccg gatttgtcgc gactaagaaa atgccgtcaa atccgctcgc      540

catgacttca ctaacgatgc ctttgaaaat cttcaagttc ttttctacta attcaaggcg      600

tgtctcacca ggtttttggt ttgctccggc gcaaatgcag acaatatcag catccttgca      660

gtcttcatat gttccgtaag atgttttgac cggttgtggc gcaaacgcct ttccgtggtt      720

taaatccatc acatcgccca ttgctttttc tttatttaca tcaatgacca caagctcatc      780

tgtgattcct tggttaatta acgcaaatgc ataactgctt ccaacaaaac ccgctccgat      840

taaagctact ttatttacat gtttgttcat cattaatcat ccttccaggg tatgtttctc      900

tttgatgtct ttttgtttgt gaagtatttc acatttatat tgtgcaacac ttcacaaact      960

tttgcaagag aaaagttttg tctgatttat gaacaaaaaa gaaaccatca ttgatggttt     1020

ctttcggtaa gtcccgtcta gccttgccct caatggggaa gagaaccgct taagcccgag     1080

tcattatata aaccatttag cacgtaatca aagccaggct gattctgacc gggcacttgg     1140

gcgctgccat tattaaaaat cacttttgcg ttggttgtat ccgtgtccgc aggcagcgtc     1200

agcgtgtaaa ttccgtctgc atttttagtc attggttttc caggccaaga tccggtcaat     1260

tcaattactc ggctcccatc atgtttatag atataagcat ttacctggct ccaatgattc     1320

ggattttgat agccgatggt tttggccgac gctggatctc ttttaacaaa actgtatttc     1380

tcggtcctcg ttacaccatc actgttcgtt ccttttaaca tgatggtgta tgttttgcca     1440

aatggatctc cttttccgat tgtgaattga tctccatcct taaacgccgt ctctggtcca     1500

ttattgattt gataaacggc ttttgttgta ttcgcatctg cacgcaaggt aatcgtcagt     1560

tgatcattga aagaatgtgt tacacctgtt ttgtaattct caaggaaaac atgaggcgct     1620

tttgcaatat catcaggata aagcacagct acagacctgg cattgatcgt gcctgtcagt     1680

ttaccatcgt tcacttgaaa tgaacccgct ccagctttat tgtcatacct gccatcaggc     1740

aattttgttg ccgtattgat agagacagag gatgaacctg catttgccag cacaacgcca     1800

tgtgagccgc gctgattcat aaatatctgg ttgtttccat tcgggttcga gagttcctca     1860

ggctgtccag ccatcacatt gtgaaatcta ttgaccgcag tgatagcctg atcttcaaat     1920

aaagcactcc cgcgatcgcc tatttggctt ttccccggga acctcacacc atttccgcct     1980

ccctcaggtc tggaaaagaa aagaggcgta ctgcctgaac gagaagctat caccgcccag     2040

cctaaacgga tatcatcatc gctcatccat gtcgagatcc ccctatgcaa gggtttattg     2100

ttttctaaaa tctgattacc aattagaatg aatatttccc aaatattaaa taataaaaca     2160

aaaaaattga aaaaagtgtt tccaccattt tttcaatttt tttataattt ttttaatctg     2220

ttatttaaat agtttatagt taaatttaca ttttcattag tccattcaat attctctcca     2280

agataactac gaactgctaa caaaattctc tccctatgtt ctaatggaga agattcagcc     2340

actgcatttc ccgcaatatc ttttggtatg attttacccg tgtccatagt taaaatcata     2400

cggcataaag ttaatataga gttggtttca tcatcctgat aattatctat taattcctct     2460

gacgaatcca taatggctct tctcacatca gaaaatggaa tatcaggtag taattcctct     2520

aagtcataat ttccgtatat tcttttattt tttcgttttg cttggtaaag cattatggtt     2580

aaatctgaat ttaattcctt ctgaggaatg tatccttgtt cataaagctc ttgtaaccat     2640

tctccataaa taaattcttg tttgggagga tgattccacg gtaccatttc ttgctgaata     2700

ataattgtta attcaatata tcgtaagttg cttttatctc ctattttttt tgaaataggt     2760

ctaatttttt gtataagtat ttctttactt tgatctgtca atggttcaga tacgacgact     2820

aaaaagtcaa gatcactatt tggttttagt ccactctcaa ctcctgatcc aaacatgtaa     2880

gtaccaataa ggttattttt taaatgtttc cgaagtattt ttttcacttt attaatttgt     2940

tcgtatgtat tcaaatatat cctcctcact attttgatta gtacctattt tatatccata     3000

gttgttaatt aaataaactt aatttagttt atttatagat ttcattggct tctaaatttt     3060

ttatctagat aataattatt ttagttaatt ttattctaga ttatatatga tatgatcttt     3120

catttccata aaactaaagt aagtgtaaac ctattcattg ttttaaaaat atctcttgcc     3180

agtcacgtta cgttattagt tatagttatt ataacatgta ttcacgaacg aaaatcgcca     3240

ttcgccagct gcaggtaaag atctcgatcc cgcgaaatta atacgactca ctatagggga     3300

attgtgagcg gataacaatt cccctctaga aagcttacat aaggaggaac tactatgcgt     3360

aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc     3420

aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg     3480

acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac tctggtaacg     3540

acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac     3600

ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat     3660

gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc     3720

attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca taagctggaa     3780

tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa     3840

gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac     3900

cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc     3960

acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag     4020

ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatg atgataataa     4080

tctagaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta     4140

tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc     4200

ctttctgcgt ttatacgttt cggtgatgaa gatcttcccg atgattaatt aattcagaac     4260

gctcggttgc cgccgggcgt tttttatgca gcaatggcaa gaacgttgct cgagggtaaa     4320

tgtgagcact cacaattcat tttgcaaaag ttgttgactt tatctacaag gtgtggcata     4380

atgtgtgtaa ttgtgagcgg ataacaatta agcttagtcg acagctagca cataaggagg     4440

aactactatg aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc     4500

tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc     4560

ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca     4620

acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact     4680

ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa     4740

gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac     4800

cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc     4860

aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga     4920

agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta     4980

caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg     5040

cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga     5100

gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg     5160

tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc     5220

aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg     5280

gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg     5340

tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa     5400

agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc     5460

caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga     5520

agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa     5580

acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct     5640

tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta     5700

caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga     5760

tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta     5820

ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg     5880

catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga     5940

gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta     6000

cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg     6060

gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc     6120

ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt     6180

caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac     6240

cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg     6300

tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc     6360

ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc     6420

tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc     6480

taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg     6540

gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat     6600

tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga     6660

atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca     6720

gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat     6780

cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc     6840

acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc     6900

ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc     6960

ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt     7020

ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc     7080

ggacttcgcg ttcgcgtaag catgcaagct aattcggtgg aaacgaggtc atcatttcct     7140

tccgaaaaaa cggttgcatt taaatcttac atatgtaata ctttcaaaga ctacatttgt     7200

aagatttgat gtttgagtcg gctgaaagat cgtacgtacc aattattgtt tcgtgattgt     7260

tcaagccata acactgtagg gatagtggaa agagtgcttc atctggttac gatcaatcaa     7320

atattcaaac ggagggagac gattttgatg aaaccagtaa cgttatacga tgtcgcagag     7380

tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct     7440

gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc     7500

gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg     7560

gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt     7620

gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa aacggcggtg     7680

cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag     7740

gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct     7800

gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg     7860

gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct     7920

gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag     7980

ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa     8040

atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg     8100

ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg     8160

ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag     8220

gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag     8280

gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg     8340

cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga     8400

caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttaggatcc     8460

tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca     8520

ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta attctgcgtg     8580

acatcccatc gatcagacca gtttttaatt tgtgtgtttc catgtgtcca gtttggaata     8640

ctcttaacct cattggaaat cgcggcataa tcactggtgg tatgattgat gaccgcgtca     8700

acaatgacct ttatgccata ttcttcagcg gctgcacaca tttctttaaa ttcttgttca     8760

gtacctaagt aacggttgcc aatttgatac gatgtcggct gatacagcca gtaccagttc     8820

gacatgcttt tatctccttg attcccttcc tttacttggt taatcggaga tgtctgaatg     8880

gctgtatatc ctgcatcatg aatatccttc atattgtgtt ttaacgtatt gaacgaccaa     8940

ttccatgcat gaagaatggt tccgcttttg atcgacggtg ctgtaagctc attcgatttg     9000

ttcgccgttt cagcactcgc agccgccggt cctgccagaa ccaaatgaaa cagcaataaa     9060

aatccagcga ataacggcag taaagaggtt ttgaatcgtt ttgcaaacat tcttgacact     9120

ccttatttga ttttttgaag acttacttcg gagtcaaaaa tccctcttac ttcattcttc     9180

cgcttcctcc tttcaaaccg atgtgaagac tggagaattt tgttaacgct tacatttaaa     9240

attatcacaa tcactctatc aaaacaactt ggcagagtga atacaaatca atgttcctat     9300

aaaaaaagct gcccgaaact gtgaacgctt ccctcgcttt ccaaacaaaa aagatgattt     9360

cttttttatt tcttctttta ccgctcttct gtaagccagc ttcacaatca cataccgaag     9420

cagaaacaga acgccgccgc agacgataaa aagaagaatc attttcagcc aagcgaagaa     9480

tgtatgggga atccccctgt cgtacatcgt aagcaccact accgcaaagg cgaccgcaat     9540

tgattctatt cgctgctgct tcacccgttt ttgcagttct tctttggtgt ctattctcgg     9600

catgagcatt ccggatgccg cagccctgat gcagtaatat aagataatga tgaacaaaaa     9660

cacaaggctt gcagcccatt ctttaaacgg aaattcaaac ataatgccgc ggatgatcac     9720

atcaagcagc gcaaatgctc ccgctatcat cccaatctcc gaccagattt tttgttcttt     9780

ttcttttact ctcttatttt tcccattcac gcctgaaagt ctattacgca gattattcat     9840

gaccttcctc ccgcactttt acgaatggct tatggcttac atgttacaat aacagcctgc     9900

gcttctgcaa tgaataactc aaaagcccgc cgtacaagcc ggcgggcagc aatcgttaaa     9960

acggccccca attgatcatg actccgatca cgataaagac aagcccgatc aagaaccaaa    10020

tcagagcaag cggcaccata aaccgcagcc acttgacata cggaataccg ctggcggcaa    10080

gcaccgccat gagaacgccg gatgtcgggt tcacacagtt gacgacccct tctccaagca    10140

taaccgcttc aaccgcaacc tgtctcgtga ttcccatcaa atcagcgagc ggcgccagga    10200

ttggaataaa tacaacggct tcgccagaac ctgaagagat gagaaaatga agcagcgcac    10260

tggcgatata catgccgatt gccccagcaa tcgggctgaa tccatccaaa agtgaagcca    10320

aagcattgac gacagtatcg agaagctttc cattttcaag gataacggaa atgcttcgtg    10380

ccatcccgac aatcagcgcc ccgtatacaa gactttggca gcccgtaatg aaggttttgg    10440

caatatcgtt cgccgcaagc ccgcctatta aaccggcaag gacagaaata aaaataaatg    10500

tcgcagccat ttgggaatct gaccagccaa gcttcaaagc gccgtataaa aatccgacaa    10560

gtgagagtcc cgccacagcc aaaatcagct tatggcgaac ggtaaacggc actgactgat    10620

cttcttcttt accggcttcg c                                              10641


<210>  7
<211>  4501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARTIFICIAL VECTOR WITHOUT SFGFP

<400>  7
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaagctgtca       60

ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt      120

tgtttaaaag cttacataag gaggaactac tatgtaacca ggcatcaaat aaaacgaaag      180

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac      240

tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatacgtt tcggtgatga      300

agatcttccc gatgattaat taattcagaa cgctcggttg ccgccgggcg ttttttatgc      360

agcaatggca agaacgttgc tcgagggtaa atgtgagcac tcacaattca ttttgcaaaa      420

gttgttgact ttatctacaa ggtgtggcat aatgtgtgta attgtgagcg gataacaatt      480

aagcttagtc gacagctagc acataaggag gaactactat gaacacgatt aacatcgcta      540

agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt      600

acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg      660

aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg      720

ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact      780

ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgcaag      840

aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca      900

gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg      960

aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg     1020

aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg     1080

ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag     1140

actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta     1200

gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac     1260

ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt     1320

tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg     1380

ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct     1440

acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat     1500

ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc     1560

cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg     1620

acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg     1680

acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt     1740

ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg     1800

ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga     1860

aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg     1920

cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga     1980

acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc     2040

cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct     2100

ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg     2160

cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc     2220

aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca     2280

atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga     2340

aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca     2400

gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc     2460

aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca     2520

ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga     2580

cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg     2640

ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg     2700

taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga     2760

acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg     2820

agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg     2880

gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg     2940

cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag     3000

tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc     3060

agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag     3120

gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa gcatgcaagc     3180

taattcggtg gaaacgaggt catcatttcc ttccgaaaaa acggttgcat ttaaatctta     3240

catatgtaat actttcaaag actacatttg taagatttga tgtttgagtc ggctgaaaga     3300

tcgtacgtac caattattgt ttcgtgattg ttcaagccat aacactgtag ggatagtgga     3360

aagagtgctt catctggtta cgatcaatca aatattcaaa cggagggaga cgattttgat     3420

gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc     3480

ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc     3540

gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc     3600

gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc     3660

ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg     3720

aagcggcgtc gaagcctgta aaacggcggt gcacaatctt ctcgcgcaac gcgtcagtgg     3780

gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac     3840

taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt     3900

ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca     3960

aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg     4020

gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag     4080

tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc     4140

gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg     4200

gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg     4260

ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt     4320

ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt     4380

ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc     4440

gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg     4500

a                                                                     4501


<210>  8
<211>  5212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant LacI-T7-sfgfp

<400>  8
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaagctgtca       60

ccggatgtgc tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt      120

tgtttaaaag cttacataag gaggaactac tatgagaaaa ggagaagaat tatttacagg      180

agttgttcca attttagtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg      240

tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac      300

tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg      360

ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga      420

aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc      480

ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt      540

taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt      600

ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa      660

cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga      720

tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga      780

tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac      840

gcatggtatg gatgaactgt acaaataacc aggcatcaaa taaaacgaaa ggctcagtcg      900

aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac      960

actggctcac cttcgggtgg gcctttctgc gtttatacgt ttcggtgatg aagatcttcc     1020

cgatgattaa ttaattcaga acgctcggtt gccgccgggc gttttttatg cagcaatggc     1080

aagaacgttg ctcgagggta aatgtgagca ctcacaattc attttgcaaa agttgttgac     1140

tttatctaca aggtgtggca taatgtgtgt aattgtgagc ggataacaat taagcttagt     1200

cgacagctag cacataagga ggaactacta tgaacacgat taacatcgct aagaacgact     1260

tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc     1320

gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct     1380

tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca     1440

agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg     1500

aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgcaa gaaatcaagc     1560

cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca     1620

atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct     1680

tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca     1740

acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc     1800

tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc     1860

atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc     1920

gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg     1980

ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt     2040

gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc     2100

gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg     2160

tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca     2220

acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg     2280

acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc     2340

ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc     2400

gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc     2460

ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa     2520

tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac     2580

caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg     2640

ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg     2700

cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct     2760

tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct     2820

cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc     2880

gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct     2940

acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg     3000

ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc     3060

tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta     3120

agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc     3180

tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga     3240

atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag     3300

ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca     3360

aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg     3420

atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt     3480

tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg     3540

cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc     3600

ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc     3660

acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa     3720

ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg     3780

accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga     3840

acctccgtga catcttagag tcggacttcg cgttcgcgta agcatgcaag ctaattcggt     3900

ggaaacgagg tcatcatttc cttccgaaaa aacggttgca tttaaatctt acatatgtaa     3960

tactttcaaa gactacattt gtaagatttg atgtttgagt cggctgaaag atcgtacgta     4020

ccaattattg tttcgtgatt gttcaagcca taacactgta gggatagtgg aaagagtgct     4080

tcatctggtt acgatcaatc aaatattcaa acggagggag acgattttga tgaaaccagt     4140

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     4200

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     4260

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     4320

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     4380

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     4440

cgaagcctgt aaaacggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     4500

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     4560

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     4620

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     4680

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     4740

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     4800

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     4860

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     4920

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     4980

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     5040

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     5100

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     5160

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt ga             5212


<210>  9
<211>  10701
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Compete Vector with sfGFP Variant

<400>  9
gttcagctca gtgatacctg cgatccctcc gcgattcacg acagccggca caccgatgta       60

cacgtcatct gcaccgtatt gcccgtccaa atatgtgctg acagttaata tgctgttttc      120

attatgaaga atggcttttg taatgcgagc aagactcatc gcaaccccat aataagtcgc      180

gccttttttc tcaatgatat ggtaagctgc gtttttcaca tcatctacaa tttggtccag      240

ctcctcttgt ttgtacgcat cgtttttctc aacgagttca ctgaccggca caccgccgac      300

attcgcgtgg ctccaaacag gaagctctgt gtcgccgtgc tctccgataa tatgcgcgtg      360

tacgttttga ggcgctgcgc caaagtattc gctcagcatg aaacggaatc tcgcagaatc      420

aagtgttgtg ccgcttccaa tcacccgctc ttttggcagg ccgctgaatt tccatgttgc      480

gtaagtcagg atatcaaccg gatttgtcgc gactaagaaa atgccgtcaa atccgctcgc      540

catgacttca ctaacgatgc ctttgaaaat cttcaagttc ttttctacta attcaaggcg      600

tgtctcacca ggtttttggt ttgctccggc gcaaatgcag acaatatcag catccttgca      660

gtcttcatat gttccgtaag atgttttgac cggttgtggc gcaaacgcct ttccgtggtt      720

taaatccatc acatcgccca ttgctttttc tttatttaca tcaatgacca caagctcatc      780

tgtgattcct tggttaatta acgcaaatgc ataactgctt ccaacaaaac ccgctccgat      840

taaagctact ttatttacat gtttgttcat cattaatcat ccttccaggg tatgtttctc      900

tttgatgtct ttttgtttgt gaagtatttc acatttatat tgtgcaacac ttcacaaact      960

tttgcaagag aaaagttttg tctgatttat gaacaaaaaa gaaaccatca ttgatggttt     1020

ctttcggtaa gtcccgtcta gccttgccct caatggggaa gagaaccgct taagcccgag     1080

tcattatata aaccatttag cacgtaatca aagccaggct gattctgacc gggcacttgg     1140

gcgctgccat tattaaaaat cacttttgcg ttggttgtat ccgtgtccgc aggcagcgtc     1200

agcgtgtaaa ttccgtctgc atttttagtc attggttttc caggccaaga tccggtcaat     1260

tcaattactc ggctcccatc atgtttatag atataagcat ttacctggct ccaatgattc     1320

ggattttgat agccgatggt tttggccgac gctggatctc ttttaacaaa actgtatttc     1380

tcggtcctcg ttacaccatc actgttcgtt ccttttaaca tgatggtgta tgttttgcca     1440

aatggatctc cttttccgat tgtgaattga tctccatcct taaacgccgt ctctggtcca     1500

ttattgattt gataaacggc ttttgttgta ttcgcatctg cacgcaaggt aatcgtcagt     1560

tgatcattga aagaatgtgt tacacctgtt ttgtaattct caaggaaaac atgaggcgct     1620

tttgcaatat catcaggata aagcacagct acagacctgg cattgatcgt gcctgtcagt     1680

ttaccatcgt tcacttgaaa tgaacccgct ccagctttat tgtcatacct gccatcaggc     1740

aattttgttg ccgtattgat agagacagag gatgaacctg catttgccag cacaacgcca     1800

tgtgagccgc gctgattcat aaatatctgg ttgtttccat tcgggttcga gagttcctca     1860

ggctgtccag ccatcacatt gtgaaatcta ttgaccgcag tgatagcctg atcttcaaat     1920

aaagcactcc cgcgatcgcc tatttggctt ttccccggga acctcacacc atttccgcct     1980

ccctcaggtc tggaaaagaa aagaggcgta ctgcctgaac gagaagctat caccgcccag     2040

cctaaacgga tatcatcatc gctcatccat gtcgagatcc ccctatgcaa gggtttattg     2100

ttttctaaaa tctgattacc aattagaatg aatatttccc aaatattaaa taataaaaca     2160

aaaaaattga aaaaagtgtt tccaccattt tttcaatttt tttataattt ttttaatctg     2220

ttatttaaat agtttatagt taaatttaca ttttcattag tccattcaat attctctcca     2280

agataactac gaactgctaa caaaattctc tccctatgtt ctaatggaga agattcagcc     2340

actgcatttc ccgcaatatc ttttggtatg attttacccg tgtccatagt taaaatcata     2400

cggcataaag ttaatataga gttggtttca tcatcctgat aattatctat taattcctct     2460

gacgaatcca taatggctct tctcacatca gaaaatggaa tatcaggtag taattcctct     2520

aagtcataat ttccgtatat tcttttattt tttcgttttg cttggtaaag cattatggtt     2580

aaatctgaat ttaattcctt ctgaggaatg tatccttgtt cataaagctc ttgtaaccat     2640

tctccataaa taaattcttg tttgggagga tgattccacg gtaccatttc ttgctgaata     2700

ataattgtta attcaatata tcgtaagttg cttttatctc ctattttttt tgaaataggt     2760

ctaatttttt gtataagtat ttctttactt tgatctgtca atggttcaga tacgacgact     2820

aaaaagtcaa gatcactatt tggttttagt ccactctcaa ctcctgatcc aaacatgtaa     2880

gtaccaataa ggttattttt taaatgtttc cgaagtattt ttttcacttt attaatttgt     2940

tcgtatgtat tcaaatatat cctcctcact attttgatta gtacctattt tatatccata     3000

gttgttaatt aaataaactt aatttagttt atttatagat ttcattggct tctaaatttt     3060

ttatctagat aataattatt ttagttaatt ttattctaga ttatatatga tatgatcttt     3120

catttccata aaactaaagt aagtgtaaac ctattcattg ttttaaaaat atctcttgcc     3180

agtcacgtta cgttattagt tatagttatt ataacatgta ttcacgaacg aaaatcgcca     3240

ttcgccagct gcaggtaaag atctcgatcc cgcgaaatta atacgactca ctatagggga     3300

attgtgagcg gataacaatt cccctctaga agctgtcacc ggatgtgctt tccggtctga     3360

tgagtccgtg aggacgaaac agcctctaca aataattttg tttaaaagct tacataagga     3420

ggaactacta tgagaaaagg agaagaatta tttacaggag ttgttccaat tttagtggaa     3480

ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca     3540

actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg     3600

ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat     3660

atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg     3720

atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat     3780

accctggtaa accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg     3840

ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc cgataaacaa     3900

aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag     3960

ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac     4020

aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat     4080

atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac     4140

aaataaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta     4200

tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc     4260

ctttctgcgt ttatacgttt cggtgatgaa gatcttcccg atgattaatt aattcagaac     4320

gctcggttgc cgccgggcgt tttttatgca gcaatggcaa gaacgttgct cgagggtaaa     4380

tgtgagcact cacaattcat tttgcaaaag ttgttgactt tatctacaag gtgtggcata     4440

atgtgtgtaa ttgtgagcgg ataacaatta agcttagtcg acagctagca cataaggagg     4500

aactactatg aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc     4560

tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc     4620

ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca     4680

acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact     4740

ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa     4800

gcgcccgaca gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac     4860

cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc     4920

aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga     4980

agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta     5040

caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg     5100

cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga     5160

gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg     5220

tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc     5280

aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg     5340

gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg     5400

tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa     5460

agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc     5520

caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga     5580

agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa     5640

acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct     5700

tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta     5760

caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga     5820

tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta     5880

ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg     5940

catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga     6000

gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta     6060

cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg     6120

gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc     6180

ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt     6240

caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac     6300

cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg     6360

tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc     6420

ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc     6480

tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc     6540

taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg     6600

gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat     6660

tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga     6720

atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca     6780

gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat     6840

cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc     6900

acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc     6960

ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc     7020

ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt     7080

ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc     7140

ggacttcgcg ttcgcgtaag catgcaagct aattcggtgg aaacgaggtc atcatttcct     7200

tccgaaaaaa cggttgcatt taaatcttac atatgtaata ctttcaaaga ctacatttgt     7260

aagatttgat gtttgagtcg gctgaaagat cgtacgtacc aattattgtt tcgtgattgt     7320

tcaagccata acactgtagg gatagtggaa agagtgcttc atctggttac gatcaatcaa     7380

atattcaaac ggagggagac gattttgatg aaaccagtaa cgttatacga tgtcgcagag     7440

tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct     7500

gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc     7560

gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg     7620

gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt     7680

gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa aacggcggtg     7740

cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag     7800

gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct     7860

gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg     7920

gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct     7980

gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag     8040

ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa     8100

atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg     8160

ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg     8220

ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag     8280

gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag     8340

gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg     8400

cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga     8460

caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttaggatcc     8520

tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca     8580

ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta attctgcgtg     8640

acatcccatc gatcagacca gtttttaatt tgtgtgtttc catgtgtcca gtttggaata     8700

ctcttaacct cattggaaat cgcggcataa tcactggtgg tatgattgat gaccgcgtca     8760

acaatgacct ttatgccata ttcttcagcg gctgcacaca tttctttaaa ttcttgttca     8820

gtacctaagt aacggttgcc aatttgatac gatgtcggct gatacagcca gtaccagttc     8880

gacatgcttt tatctccttg attcccttcc tttacttggt taatcggaga tgtctgaatg     8940

gctgtatatc ctgcatcatg aatatccttc atattgtgtt ttaacgtatt gaacgaccaa     9000

ttccatgcat gaagaatggt tccgcttttg atcgacggtg ctgtaagctc attcgatttg     9060

ttcgccgttt cagcactcgc agccgccggt cctgccagaa ccaaatgaaa cagcaataaa     9120

aatccagcga ataacggcag taaagaggtt ttgaatcgtt ttgcaaacat tcttgacact     9180

ccttatttga ttttttgaag acttacttcg gagtcaaaaa tccctcttac ttcattcttc     9240

cgcttcctcc tttcaaaccg atgtgaagac tggagaattt tgttaacgct tacatttaaa     9300

attatcacaa tcactctatc aaaacaactt ggcagagtga atacaaatca atgttcctat     9360

aaaaaaagct gcccgaaact gtgaacgctt ccctcgcttt ccaaacaaaa aagatgattt     9420

cttttttatt tcttctttta ccgctcttct gtaagccagc ttcacaatca cataccgaag     9480

cagaaacaga acgccgccgc agacgataaa aagaagaatc attttcagcc aagcgaagaa     9540

tgtatgggga atccccctgt cgtacatcgt aagcaccact accgcaaagg cgaccgcaat     9600

tgattctatt cgctgctgct tcacccgttt ttgcagttct tctttggtgt ctattctcgg     9660

catgagcatt ccggatgccg cagccctgat gcagtaatat aagataatga tgaacaaaaa     9720

cacaaggctt gcagcccatt ctttaaacgg aaattcaaac ataatgccgc ggatgatcac     9780

atcaagcagc gcaaatgctc ccgctatcat cccaatctcc gaccagattt tttgttcttt     9840

ttcttttact ctcttatttt tcccattcac gcctgaaagt ctattacgca gattattcat     9900

gaccttcctc ccgcactttt acgaatggct tatggcttac atgttacaat aacagcctgc     9960

gcttctgcaa tgaataactc aaaagcccgc cgtacaagcc ggcgggcagc aatcgttaaa    10020

acggccccca attgatcatg actccgatca cgataaagac aagcccgatc aagaaccaaa    10080

tcagagcaag cggcaccata aaccgcagcc acttgacata cggaataccg ctggcggcaa    10140

gcaccgccat gagaacgccg gatgtcgggt tcacacagtt gacgacccct tctccaagca    10200

taaccgcttc aaccgcaacc tgtctcgtga ttcccatcaa atcagcgagc ggcgccagga    10260

ttggaataaa tacaacggct tcgccagaac ctgaagagat gagaaaatga agcagcgcac    10320

tggcgatata catgccgatt gccccagcaa tcgggctgaa tccatccaaa agtgaagcca    10380

aagcattgac gacagtatcg agaagctttc cattttcaag gataacggaa atgcttcgtg    10440

ccatcccgac aatcagcgcc ccgtatacaa gactttggca gcccgtaatg aaggttttgg    10500

caatatcgtt cgccgcaagc ccgcctatta aaccggcaag gacagaaata aaaataaatg    10560

tcgcagccat ttgggaatct gaccagccaa gcttcaaagc gccgtataaa aatccgacaa    10620

gtgagagtcc cgccacagcc aaaatcagct tatggcgaac ggtaaacggc actgactgat    10680

cttcttcttt accggcttcg c                                              10701


<210>  10
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SUPER FOLDER GFP

<400>  10
atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt       60

gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt      120

aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg      180

gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag      240

catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt      300

aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta      360

aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag      420

ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc      480

attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat      540

cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat      600

ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg      660

ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgatga      720


<210>  11
<211>  1083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LACI FROM PDR111

<400>  11
atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt       60

tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg      120

gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag      180

tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc      240

gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa      300

cgaagcggcg tcgaagcctg taaaacggcg gtgcacaatc ttctcgcgca acgcgtcagt      360

gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc      420

actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt      480

ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag      540

caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc      600

tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg      660

agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact      720

gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc      780

gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca      840

tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc      900

gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc      960

gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc     1020

gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag     1080

tga                                                                   1083


<210>  12
<211>  2652
<212>  DNA
<213>  T7 PHAGE

<400>  12
atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg       60

ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag      120

catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa      180

gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag      240

atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg      300

acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag      360

accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca      420

atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag      480

cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa      540

gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg      600

tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc      660

attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac      720

tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg      780

ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc      840

attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac      900

agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt      960

aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta     1020

atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc     1080

ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct     1140

gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc     1200

atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg     1260

gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc     1320

aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg     1380

aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag     1440

ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact     1500

tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg     1560

gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc     1620

tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac     1680

ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag     1740

attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag     1800

aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg     1860

ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg     1920

tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat     1980

tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg     2040

atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag     2100

tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc     2160

aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag     2220

aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc     2280

attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct     2340

aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag     2400

aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac     2460

gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat     2520

gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa     2580

atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc     2640

gcgttcgcgt aa                                                         2652


<210>  13
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IPTG-INDUCIBLE PROMOTER

<400>  13
ggtaaatgtg agcactcaca attcattttg caaaagttgt tgactttatc tacaaggtgt       60

ggcataatgt gtgtaattgt gagcggataa caattaagct tagtcgaca                  109


<210>  14
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hybrid T7 promoter with lac operators SEQ ID NO 14

<400>  14
taatacgact cactataggg gaattgtgag cggataacaa ttcccct                     47


<210>  15
<211>  94
<212>  DNA
<213>  Bacillus licheniformis

<400>  15
cggtggaaac gaggtcatca tttccttccg aaaaaacggt tgcatttaaa tcttacatat       60

gtaatacttt caaagactac atttgtaaga tttg                                   94


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic RBS in pMF35

<400>  16
aagcttacat aaggaggaac tact                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Slightly modified MF001

<400>  17
gctagcacat aaggaggaac tact                                              24


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBS driving expression of lacI

<400>  18
ttcaaacgga gggagacgat tttg                                              24


<210>  19
<211>  129
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TERMINATOR

<400>  19
ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt       60

gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct      120

gcgtttata                                                              129


<210>  20
<211>  717
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized sfGFP

<400>  20
atgagaaaag gagaagaatt atttacagga gttgttccaa ttttagtgga actggatggt       60

gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt      120

aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg      180

gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag      240

catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt      300

aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta      360

aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag      420

ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc      480

attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat      540

cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat      600

ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg      660

ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaataa         717


<210>  21
<211>  3075
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ]-galactosidase enzyme

<400>  21
atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct       60

ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc      120

gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc      180

tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct      240

gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc      300

tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg      360

acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg      420

cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc      480

ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc      540

ggagaaaacc gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat      600

caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact      660

acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta      720

ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct      780

ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc      840

gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa      900

ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac      960

ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat     1020

ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat     1080

catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg     1140

aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac     1200

acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc     1260

atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc     1320

gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg     1380

aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat     1440

ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt     1500

tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc     1560

atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc     1620

cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat     1680

ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat     1740

gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc     1800

cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa     1860

gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc     1920

agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat     1980

ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg     2040

attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc     2100

gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag     2160

tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat     2220

ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac     2280

cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg     2340

ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc     2400

cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa     2460

gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct     2520

cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat     2580

ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg     2640

gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga     2700

ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat     2760

ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc     2820

gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc     2880

agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa     2940

gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg     3000

agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc     3060

tggtgtcaaa aataa                                                      3075


<210>  22
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Self-cleaving ribozyme

<400>  22
agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca       60

aataattttg tttaa                                                        75


