                         SEQUENCE LISTING

<110>  BRODY, Richard S.
 
<120>  Pegylated Growth Hormone Antagonists

<130>  0136067-0715891

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  567
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WThGH (DNA) Human Growth Hormone

<400>  1
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc catcgtctgc       60

accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca aggaacagaa      120

gtattcattc ctgcagaacc cccagacctc cctctgtttc tcagagtcat tccgacaccc      180

tccaacaggg aggaaacaca acagaaatcc aacctagagc tgctccgatc tccctgctgc      240

tcatccagtc gtggctggag cccgtgcagt tcctcaggag tgtcttgcca acagcctggt      300

gtacggcgcc tctgacagca acgtctatga cctcctaaag gacctgagga aggcatccaa      360

acgctgatgg ggaggctgga agatggcagc ccccggactg ggcaatcttc aagcagacct      420

acagcaagtt cgacacaaac tcacacaacg atgacgcact actaagaact acgggctgct      480

ctactgcttc aggaaggaca tggacaaggt cgagacattc ctcgcatcgt gcagtgccgc      540

tctgtggagg gcagctgtgg cttctag                                          567


<210>  2
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WThGH (amino acid) Human Growth Hormone (Mature Form)

<400>  2

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu 
    50                  55                  60                  


Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu 
65                  70                  75                  80  


Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe 
                85                  90                  95      


Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Asp Leu Leu 
            100                 105                 110         


Lys Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg Leu Glu Asp 
        115                 120                 125             


Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe 
    130                 135                 140                 


Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu 
145                 150                 155                 160 


Leu Tyr Cys Phe Arg Lys Asp Met Lys Val Glu Thr Phe Leu Arg Ile 
                165                 170                 175     


Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185             


<210>  3
<211>  567
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K (DNA) Human Growth Hormone Antagonist G120K

<400>  3
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc catcgtctgc       60

accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca aggaacagaa      120

gtattcattc ctgcagaacc cccagacctc cctctgtttc tcagagtcat tccgacaccc      180

tccaacaggg aggaaacaca acagaaatcc aacctagagc tgctccgatc tccctgctgc      240

tcatccagtc gtggctggag cccgtgcagt tcctcaggag tgtcttgcca acagcctggt      300

gtacggcgcc tctgacagca acgtctatga cctcctaaag gacctgagga aaagatccaa      360

acgctgatgg ggaggctgga agatggcagc ccccggactg ggcaatcttc aagcagacct      420

acagcaagtt cgacacaaac tcacacaacg atgacgcact actaagaact acgggctgct      480

ctactgcttc aggaaggaca tggacaaggt cgagacattc ctcgcatcgt gcagtgccgc      540

tctgtggagg gcagctgtgg cttctag                                          567


<210>  4
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K (amino acid) Human Growth Hormone Antagonist G120K (Mature 
       Form)

<400>  4

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg Glu 
    50                  55                  60                  


Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu Leu 
65                  70                  75                  80  


Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val Phe 
                85                  90                  95      


Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Asp Leu Leu 
            100                 105                 110         


Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu Glu Asp 
        115                 120                 125             


Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser Lys Phe 
    130                 135                 140                 


Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr Gly Leu 
145                 150                 155                 160 


Leu Tyr Cys Phe Arg Lys Asp Met Lys Val Glu Thr Phe Leu Arg Ile 
                165                 170                 175     


Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185             


<210>  5
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-T3C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagonist

<400>  5
atggcgcatc accaccacca tcacggcagc agcggcgaga acctgtactt ccagggtgga       60

agctcgggat tcccatgcat tcccttatcc aggctttttg acaacgctat gctccgcgcc      120

catcgtctgc accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca      180

aaggaacaga agtattcatt cctgcagaac ccccagacct ccctctgttt ctcagagtct      240

attccgacac cctccaacag ggaggaaaca caacagaaat ccaacctaga gctgctccgc      300

atctccctgc tgctcatcca gtcgtggctg gagcccgtgc agttcctcag gagtgtcttc      360

gccaacagcc tggtgtacgg cgcctctgac agcaacgtct atgacctcct aaaggaccta      420

gaggaaaaga tccaaacgct gatggggagg ctggaagatg gcagcccccg gactgggcag      480

atcttcaagc agacctacag caagttcgac acaaactcac acaacgatga cgcactactc      540

aagaactacg ggctgctcta ctgcttcagg aaggacatgg acaaggtcga gacattcctg      600

cgcatcgtgc agtgccgctc tgtggagggc agctgtggct tctag                      645


<210>  6
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-T3C (amino acid) Synthetic Constructs / Mutant Human Growth
       Hormone Antagonists

<400>  6

Met Ala His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Gly Ser Ser Gly Phe Pro Cys Ile Pro Leu Ser Arg Leu 
            20                  25                  30          


Phe Asp Asn Ala Met Leu Arg Ala His Arg Leu His Gln Leu Ala Phe 
        35                  40                  45              


Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr Ile Pro Lys Glu Gln Lys 
    50                  55                  60                  


Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser Leu Cys Phe Ser Glu Ser 
65                  70                  75                  80  


Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr Gln Gln Lys Ser Asn Leu 
                85                  90                  95      


Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile Gln Ser Trp Leu Glu Pro 
            100                 105                 110         


Val Gln Phe Leu Arg Ser Val Phe Ala Asn Ser Leu Val Tyr Gly Ala 
        115                 120                 125             


Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys Asp Leu Glu Glu Lys Ile 
    130                 135                 140                 


Gln Thr Leu Met Gly Arg Leu Glu Asp Gly Ser Pro Arg Thr Gly Gln 
145                 150                 155                 160 


Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr Asn Ser His Asn Asp 
                165                 170                 175     


Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp 
            180                 185                 190         


Met Asp Lys Val Glu Thr Phe Leu Arg Ile Val Gln Cys Arg Ser Val 
        195                 200                 205             


Glu Gly Ser Cys Gly Phe 
    210                 


<210>  7
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-E39C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagnonist

<400>  7
atggcgcatc accaccacca tcacggcagc agcggcgaga acctgtactt ccagggtgga       60

agctcgggat tcccaaccat tcccttatcc aggctttttg acaacgctat gctccgcgcc      120

catcgtctgc accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca      180

aagtgccaga agtattcatt cctgcagaac ccccagacct ccctctgttt ctcagagtct      240

attccgacac cctccaacag ggaggaaaca caacagaaat ccaacctaga gctgctccgc      300

atctccctgc tgctcatcca gtcgtggctg gagcccgtgc agttcctcag gagtgtcttc      360

gccaacagcc tggtgtacgg cgcctctgac agcaacgtct atgacctcct aaaggaccta      420

gaggaaaaga tccaaacgct gatggggagg ctggaagatg gcagcccccg gactgggcag      480

atcttcaagc agacctacag caagttcgac acaaactcac acaacgatga cgcactactc      540

aagaactacg ggctgctcta ctgcttcagg aaggacatgg acaaggtcga gacattcctg      600

cgcatcgtgc agtgccgctc tgtggagggc agctgtggct tctag                      645


<210>  8
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-E39C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  8

Met Ala His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Gly Ser Ser Gly Phe Pro Thr Ile Pro Leu Ser Arg Leu 
            20                  25                  30          


Phe Asp Asn Ala Met Leu Arg Ala His Arg Leu His Gln Leu Ala Phe 
        35                  40                  45              


Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr Ile Pro Lys Cys Gln Lys 
    50                  55                  60                  


Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser Leu Cys Phe Ser Glu Ser 
65                  70                  75                  80  


Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr Gln Gln Lys Ser Asn Leu 
                85                  90                  95      


Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile Gln Ser Trp Leu Glu Pro 
            100                 105                 110         


Val Gln Phe Leu Arg Ser Val Phe Ala Asn Ser Leu Val Tyr Gly Ala 
        115                 120                 125             


Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys Asp Leu Glu Glu Lys Ile 
    130                 135                 140                 


Gln Thr Leu Met Gly Arg Leu Glu Asp Gly Ser Pro Arg Thr Gly Gln 
145                 150                 155                 160 


Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr Asn Ser His Asn Asp 
                165                 170                 175     


Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp 
            180                 185                 190         


Met Asp Lys Val Glu Thr Phe Leu Arg Ile Val Gln Cys Arg Ser Val 
        195                 200                 205             


Glu Gly Ser Cys Gly Phe 
    210                 


<210>  9
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-P48C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagonists

<400>  9
atggcgcatc accaccacca tcacggcagc agcggcgaga acctgtactt ccagggtgga       60

agctcgggat tcccaaccat tcccttatcc aggctttttg acaacgctat gctccgcgcc      120

catcgtctgc accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca      180

aaggaacaga agtattcatt cctgcagaac tgtcagacct ccctctgttt ctcagagtct      240

attccgacac cctccaacag ggaggaaaca caacagaaat ccaacctaga gctgctccgc      300

atctccctgc tgctcatcca gtcgtggctg gagcccgtgc agttcctcag gagtgtcttc      360

gccaacagcc tggtgtacgg cgcctctgac agcaacgtct atgacctcct aaaggaccta      420

gaggaaaaga tccaaacgct gatggggagg ctggaagatg gcagcccccg gactgggcag      480

atcttcaagc agacctacag caagttcgac acaaactcac acaacgatga cgcactactc      540

aagaactacg ggctgctcta ctgcttcagg aaggacatgg acaaggtcga gacattcctg      600

cgcatcgtgc agtgccgctc tgtggagggc agctgtggct tctag                      645


<210>  10
<211>  70
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-P48C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  10

Gln Thr Leu Met Gly Arg Leu Glu Asp Gly Ser Pro Arg Thr Gly Gln 
1               5                   10                  15      


Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr Asn Ser His Asn Asp 
            20                  25                  30          


Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp 
        35                  40                  45              


Met Asp Lys Val Glu Thr Phe Leu Arg Ile Val Gln Cys Arg Ser Val 
    50                  55                  60                  


Glu Gly Ser Cys Gly Phe 
65                  70  


<210>  11
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-Q69C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagonists

<400>  11
atggcgcatc accaccacca tcacggcagc agcggcgaga acctgtactt ccagggtgga       60

agctcgggat tcccaaccat tcccttatcc aggctttttg acaacgctat gctccgcgcc      120

catcgtctgc accagctggc ctttgacacc taccaggagt ttgaagaagc ctatatccca      180

aaggaacaga agtattcatt cctgcagaac ccccagacct ccctctgttt ctcagagtct      240

attccgacac cctccaacag ggaggaaaca cagtgcaaat ccaacctaga gctgctccgc      300

atctccctgc tgctcatcca gtcgtggctg gagcccgtgc agttcctcag gagtgtcttc      360

gccaacagcc tggtgtacgg cgcctctgac agcaacgtct atgacctcct aaaggaccta      420

gaggaaaaga tccaaacgct gatggggagg ctggaagatg gcagcccccg gactgggcag      480

atcttcaagc agacctacag caagttcgac acaaactcac acaacgatga cgcactactc      540

aagaactacg ggctgctcta ctgcttcagg aaggacatgg acaaggtcga gacattcctg      600

cgcatcgtgc agtgccgctc tgtggagggc agctgtggct tctag                      645


<210>  12
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-Q69C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  12

Met Ala His His His His His His Gly Ser Ser Gly Glu Asn Leu Tyr 
1               5                   10                  15      


Phe Gln Gly Gly Ser Ser Gly Phe Pro Thr Ile Pro Leu Ser Arg Leu 
            20                  25                  30          


Phe Asp Asn Ala Met Leu Arg Ala His Arg Leu His Gln Leu Ala Phe 
        35                  40                  45              


Asp Thr Tyr Gln Glu Phe Glu Glu Ala Tyr Ile Pro Lys Glu Gln Lys 
    50                  55                  60                  


Tyr Ser Phe Leu Gln Asn Pro Gln Thr Ser Leu Cys Phe Ser Glu Ser 
65                  70                  75                  80  


Ile Pro Thr Pro Ser Asn Arg Glu Glu Thr Gln Cys Lys Ser Asn Leu 
                85                  90                  95      


Glu Leu Leu Arg Ile Ser Leu Leu Leu Ile Gln Ser Trp Leu Glu Pro 
            100                 105                 110         


Val Gln Phe Leu Arg Ser Val Phe Ala Asn Ser Leu Val Tyr Gly Ala 
        115                 120                 125             


Ser Asp Ser Asn Val Tyr Asp Leu Leu Lys Asp Leu Glu Glu Lys Ile 
    130                 135                 140                 


Gln Thr Leu Met Gly Arg Leu Glu Asp Gly Ser Pro Arg Thr Gly Gln 
145                 150                 155                 160 


Ile Phe Lys Gln Thr Tyr Ser Lys Phe Asp Thr Asn Ser His Asn Asp 
                165                 170                 175     


Asp Ala Leu Leu Lys Asn Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp 
            180                 185                 190         


Met Asp Lys Val Glu Thr Phe Leu Arg Ile Val Gln Cys Arg Ser Val 
        195                 200                 205             


Glu Gly Ser Cys Gly Phe 
    210                 


<210>  13
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-N99C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagonists

<400>  13
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgcctgcagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagacctaca gcaagttcga cacaaactca cacaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  14
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-N99C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  14

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Cys Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


<210>  15
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-T142C (DNA) Synthetic Constructs / Mutant Human Growth 
       Hormone Antagonists

<400>  15
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgccaacagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagtgctaca gcaagttcga cacaaactca cacaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  16
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-T142C (amino acid) Synthetic Constructs / Mutant Human  
       Growth Hormone Antagonists

<400>  16

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Cys Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


<210>  17
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-H151C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  17
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgccaacagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagacctaca gcaagttcga cacaaactca tgcaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  18
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-H151C (amino acid) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  18

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser Cys Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


<210>  19
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-N99C-dPEGX-H151C (DNA) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  19
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgcctgcagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagacctaca gcaagttcga cacaaactca tgcaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  20
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-N99C-dPEGX-H151C (amino acid) Synthetic Constructs / Mutant
       Human Growth Hormone Antagonists

<400>  20

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Cys Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser Cys Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


<210>  21
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-T142C-dPEGX-N99C (DNA) Synthetic Constructs / Mutant Human 
       Growth Hormone Antagonists

<400>  21
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgcctgcagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagtgctaca gcaagttcga cacaaactca cacaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  22
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-T142C-dPEGX-N99C (amino acid) Synthetic Constructs / Mutant
       Human Growth Hormone Antagonists

<400>  22

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Cys Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Cys Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


<210>  23
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G120K-T142C-dPEGX-H151C (DNA) Synthetic Constructs / Mutant Human
       Growth Hormone Antagonists

<400>  23
ttcccaacca ttcccttatc caggcttttt gacaacgcta tgctccgcgc ccatcgtctg       60

caccagctgg cctttgacac ctaccaggag tttgaagaag cctatatccc aaaggaacag      120

aagtattcat tcctgcagaa cccccagacc tccctctgtt tctcagagtc tattccgaca      180

ccctccaaca gggaggaaac acaacagaaa tccaacctag agctgctccg catctccctg      240

ctgctcatcc agtcgtggct ggagcccgtg cagttcctca ggagtgtctt cgccaacagc      300

ctggtgtacg gcgcctctga cagcaacgtc tatgacctcc taaaggacct agaggaaaag      360

atccaaacgc tgatggggag gctggaagat ggcagccccc ggactgggca gatcttcaag      420

cagtgctaca gcaagttcga cacaaactca tgcaacgatg acgcactact caagaactac      480

gggctgctct actgcttcag gaaggacatg gacaaggtcg agacattcct gcgcatcgtg      540

cagtgccgct ctgtggaggg cagctgtggc ttctag                                576


<210>  24
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G120K-T142C-dPEGX-H151C (amino acid) Synthetic Constructs / 
       Mutant Human Growth Hormone Antagonists

<400>  24

Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu Arg 
1               5                   10                  15      


Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe Glu 
            20                  25                  30          


Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn Pro 
        35                  40                  45              


Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn Arg 
    50                  55                  60                  


Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser Leu 
65                  70                  75                  80  


Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser Val 
                85                  90                  95      


Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr Asp 
            100                 105                 110         


Leu Leu Lys Asp Leu Glu Glu Lys Ile Gln Thr Leu Met Gly Arg Leu 
        115                 120                 125             


Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Cys Tyr Ser 
    130                 135                 140                 


Lys Phe Asp Thr Asn Ser Cys Asn Asp Asp Ala Leu Leu Lys Asn Tyr 
145                 150                 155                 160 


Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr Phe 
                165                 170                 175     


Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe 
            180                 185                 190     


