                         SEQUENCE LISTING

<110>  OncoSec Medical Incorporated
       Canton, David A.
 
<120>  MULTIGENE CONSTRUCT FOR IMMUNE-MODULATORY PROTEIN EXPRESSION AND 
       METHODS OF USE

<130>  066914/541462

<150>  62/778,027
<151>  2018-12-11

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  6752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid sequence with recombinant human gene sequences within

<400>  1
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca       60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg      120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc      480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc      540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      660

cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga      720

agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc      780

cgtgccaaga gtgacgtaag taccgcctat agactctata ggcacacccc tttggctctt      840

atgcatgcta tactgttttt ggcttggggc ctatacaccc ccgcttcctt atgctatagg      900

tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca ctccaacggt      960

ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag     1020

ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtcgtcgacg     1080

gtatcgataa gcttgatatc gaattcacgt gggcccggta ccaccatgtg gccccctggg     1140

tcagcctccc agccaccgcc ctcacctgcc gcggccacag gtctgcatcc agcggctcgc     1200

cctgtgtccc tgcagtgccg gctcagcatg tgtccagcgc gcagcctcct ccttgtggct     1260

accctggtcc tcctggacca cctcagtttg gccagaaacc tccccgtggc cactccagac     1320

ccaggaatgt tcccatgcct tcaccactcc caaaacctgc tgagggccgt cagcaacatg     1380

ctccagaagg ccagacaaac tctagaattt tacccttgca cttctgaaga gattgatcat     1440

gaagatatca caaaagataa aaccagcaca gtggaggcct gtttaccatt ggaattaacc     1500

aagaatgaga gttgcctaaa ttccagagag acctctttca taactaatgg gagttgcctg     1560

gcctccagaa agacctcttt tatgatggcc ctgtgcctta gtagtattta tgaagacttg     1620

aagatgtacc aggtggagtt caagaccatg aatgcaaagc ttctgatgga tcctaagagg     1680

cagatctttc tagatcaaaa catgctggca gttattgatg agctgatgca ggccctgaat     1740

ttcaacagtg agactgtgcc acaaaaatcc tcccttgaag aaccggattt ttataaaact     1800

aaaatcaagc tctgcatact tcttcatgct ttcagaattc gggcagtgac tattgataga     1860

gtgatgagct atctgaatgc ttccggatct ggggccacca acttttcatt gctcaagcag     1920

gcgggcgatg tggaggaaaa ccctggcccc tgtcaccagc agttggtcat ctcttggttt     1980

tccctggttt ttctggcatc tcccctcgtg gccatatggg aactgaagaa agatgtttat     2040

gtcgtagaat tggattggta tccggatgcc cctggagaaa tggtggtcct cacctgtgac     2100

acccctgaag aagatggtat cacctggacc ttggaccaga gcagtgaggt cttaggctct     2160

ggcaaaaccc tgaccatcca agtcaaagag tttggagatg ctggccagta cacctgtcac     2220

aaaggaggcg aggttctaag ccattcgctc ctgctgcttc acaaaaagga agatggaatt     2280

tggtccactg atattttaaa ggaccagaaa gaacccaaaa ataagacctt tctaagatgc     2340

gaggccaaga attattctgg acgtttcacc tgctggtggc tgacgacaat cagtactgat     2400

ttgacattca gtgtcaaaag cagcagaggc tcttctgacc cccaaggggt gacgtgcgga     2460

gctgctacac tctctgcaga gagagtcaga ggggacaaca aggagtatga gtactcagtg     2520

gagtgccagg aggacagtgc ctgcccagct gctgaggaga gtctgcccat tgaggtcatg     2580

gtggatgccg ttcacaagct caagtatgaa aactacacca gcagcttctt catcagggac     2640

atcatcaaac ctgacccacc caagaacttg cagctgaagc cattaaagaa ttctcggcag     2700

gtggaggtca gctgggagta ccctgacacc tggagtactc cacattccta cttctccctg     2760

acattctgcg ttcaggtcca gggcaagagc aagagagaaa agaaagatag agtcttcacg     2820

gacaagacct cagccacggt catctgccgc aaaaatgcca gcattagcgt gcgggcccag     2880

gaccgctact atagctcatc ttggagcgaa tgggcatctg tgccctgcag tggatctggg     2940

gccaccaact tttcattgct caagcaggcg ggcgatgtgg aggaaaaccc tggccccgag     3000

acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac tggtgacact     3060

caggattgca gcttccagca ttcacccata tcatcagatt ttgcagtaaa gatcagggaa     3120

ctctccgatt atctccttca agactacccc gtaacagtgg cctccaattt gcaagacgaa     3180

gagctttgtg gtgccctctg gcggctcgtt ttggcccaaa ggtggatgga acggcttaag     3240

acagtcgctg gcagcaagat gcaagggttg ctcgaacgag tcaatacaga gatccatttt     3300

gtaaccaagt gtgcatttca accgccgcca agctgccttc gctttgttca gacgaatata     3360

agtagactgt tgcaggaaac ctccgagcaa ctcgtagccc tgaagccctg gattacacgg     3420

caaaatttca gtcggtgcct tgagcttcag tgtcagcctg atagtagtac cttgcctccg     3480

ccatggtccc ccaggcctct tgaagctaca gctccgacag cccctcagcc gggcagtagt     3540

ggtagttctg gagccagggg gccggagagc cgcctgcttg agttctacct cgccatgcct     3600

ttcgcgacac ccatggaagc agagctggcc cgcaggagcc tggcccagga tgccccaccg     3660

cttcccgtgc caggggtgct tctgaaggag ttcactgtgt ccggcaacat actgactatc     3720

cgactgactg ctgcagacca ccgccaactg cagctctcca tcagctcctg tctccagcag     3780

ctttccctgt tgatgtggat cacgcagtgc tttctgcccg tgtttttggc tcagcctccc     3840

tcagggcaga ggcgctaagg ccgcggatcc agatcttttt ccctctgcca aaaattatgg     3900

ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat     3960

tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa     4020

tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccattcttc     4080

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     4140

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     4200

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     4260

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     4320

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     4380

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     4440

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     4500

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     4560

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     4620

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     4680

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt     4740

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     4800

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     4860

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     4920

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     4980

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     5040

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcg gggggggggg     5100

gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc     5160

atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca     5220

gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt     5280

gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa     5340

gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca     5400

tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga     5460

aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga     5520

tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc     5580

tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag     5640

aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg     5700

tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga     5760

cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc     5820

aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc     5880

tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg     5940

ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc     6000

tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca     6060

tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc     6120

catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac     6180

gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt     6240

tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac     6300

acaacgtggc tttccccccc cccccattat tgaagcattt atcagggtta ttgtctcatg     6360

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     6420

ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa     6480

aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc     6540

tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga     6600

caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg     6660

gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc     6720

gtaaggagaa aataccgcat cagattggct at                                   6752


<210>  2
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human recombinant protein

<400>  2

Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly 
        


<210>  3
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant human protein

<400>  3

Pro Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val Phe Leu 
1               5                   10                  15      


Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val Tyr Val 
            20                  25                  30          


Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val Val Leu 
        35                  40                  45              


Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu Asp Gln 
    50                  55                  60                  


Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln Val Lys 
65                  70                  75                  80  


Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly Glu Val 
                85                  90                  95      


Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly Ile Trp 
            100                 105                 110         


Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys Thr Phe 
        115                 120                 125             


Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys Trp Trp 
    130                 135                 140                 


Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser Ser Arg 
145                 150                 155                 160 


Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr Leu Ser 
                165                 170                 175     


Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser Val Glu 
            180                 185                 190         


Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu Pro Ile 
        195                 200                 205             


Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn Tyr Thr 
    210                 215                 220                 


Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro Lys Asn 
225                 230                 235                 240 


Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val Ser Trp 
                245                 250                 255     


Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser Leu Thr 
            260                 265                 270         


Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys Asp Arg 
        275                 280                 285             


Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys Asn Ala 
    290                 295                 300                 


Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser Trp Ser 
305                 310                 315                 320 


Glu Trp Ala Ser Val Pro Cys Ser Gly Ser Gly Ala Thr Asn Phe Ser 
                325                 330                 335     


Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly 
            340                 345                 


<210>  4
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  recombinant human protein

<400>  4

Pro Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Thr Gln Asp Cys Ser Phe Gln His Ser Pro Ile 
            20                  25                  30          


Ser Ser Asp Phe Ala Val Lys Ile Arg Glu Leu Ser Asp Tyr Leu Leu 
        35                  40                  45              


Gln Asp Tyr Pro Val Thr Val Ala Ser Asn Leu Gln Asp Glu Glu Leu 
    50                  55                  60                  


Cys Gly Ala Leu Trp Arg Leu Val Leu Ala Gln Arg Trp Met Glu Arg 
65                  70                  75                  80  


Leu Lys Thr Val Ala Gly Ser Lys Met Gln Gly Leu Leu Glu Arg Val 
                85                  90                  95      


Asn Thr Glu Ile His Phe Val Thr Lys Cys Ala Phe Gln Pro Pro Pro 
            100                 105                 110         


Ser Cys Leu Arg Phe Val Gln Thr Asn Ile Ser Arg Leu Leu Gln Glu 
        115                 120                 125             


Thr Ser Glu Gln Leu Val Ala Leu Lys Pro Trp Ile Thr Arg Gln Asn 
    130                 135                 140                 


Phe Ser Arg Cys Leu Glu Leu Gln Cys Gln Pro Asp Ser Ser Thr Leu 
145                 150                 155                 160 


Pro Pro Pro Trp Ser Pro Arg Pro Leu Glu Ala Thr Ala Pro Thr Ala 
                165                 170                 175     


Pro Gln Pro Gly Ser Ser Gly Ser Ser Gly Ala Arg Gly Pro Glu Ser 
            180                 185                 190         


Arg Leu Leu Glu Phe Tyr Leu Ala Met Pro Phe Ala Thr Pro Met Glu 
        195                 200                 205             


Ala Glu Leu Ala Arg Arg Ser Leu Ala Gln Asp Ala Pro Pro Leu Pro 
    210                 215                 220                 


Val Pro Gly Val Leu Leu Lys Glu Phe Thr Val Ser Gly Asn Ile Leu 
225                 230                 235                 240 


Thr Ile Arg Leu Thr Ala Ala Asp His Arg Gln Leu Gln Leu Ser Ile 
                245                 250                 255     


Ser Ser Cys Leu Gln Gln Leu Ser Leu Leu Met Trp Ile Thr Gln Cys 
            260                 265                 270         


Phe Leu Pro Val Phe Leu Ala Gln Pro Pro Ser Gly Gln Arg Arg 
        275                 280                 285         


<210>  5
<211>  825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hIL12p35-[GSG Hinge]-P2A

<400>  5
atgtggcccc ctgggtcagc ctcccagcca ccgccctcac ctgccgcggc cacaggtctg       60

catccagcgg ctcgccctgt gtccctgcag tgccggctca gcatgtgtcc agcgcgcagc      120

ctcctccttg tggctaccct ggtcctcctg gaccacctca gtttggccag aaacctcccc      180

gtggccactc cagacccagg aatgttccca tgccttcacc actcccaaaa cctgctgagg      240

gccgtcagca acatgctcca gaaggccaga caaactctag aattttaccc ttgcacttct      300

gaagagattg atcatgaaga tatcacaaaa gataaaacca gcacagtgga ggcctgttta      360

ccattggaat taaccaagaa tgagagttgc ctaaattcca gagagacctc tttcataact      420

aatgggagtt gcctggcctc cagaaagacc tcttttatga tggccctgtg ccttagtagt      480

atttatgaag acttgaagat gtaccaggtg gagttcaaga ccatgaatgc aaagcttctg      540

atggatccta agaggcagat ctttctagat caaaacatgc tggcagttat tgatgagctg      600

atgcaggccc tgaatttcaa cagtgagact gtgccacaaa aatcctccct tgaagaaccg      660

gatttttata aaactaaaat caagctctgc atacttcttc atgctttcag aattcgggca      720

gtgactattg atagagtgat gagctatctg aatgcttccg gatctggggc caccaacttt      780

tcattgctca agcaggcggg cgatgtggag gaaaaccctg gcccc                      825


<210>  6
<211>  1047
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hIL12p40-[GSG Hinge]-P2A

<400>  6
tgtcaccagc agttggtcat ctcttggttt tccctggttt ttctggcatc tcccctcgtg       60

gccatatggg aactgaagaa agatgtttat gtcgtagaat tggattggta tccggatgcc      120

cctggagaaa tggtggtcct cacctgtgac acccctgaag aagatggtat cacctggacc      180

ttggaccaga gcagtgaggt cttaggctct ggcaaaaccc tgaccatcca agtcaaagag      240

tttggagatg ctggccagta cacctgtcac aaaggaggcg aggttctaag ccattcgctc      300

ctgctgcttc acaaaaagga agatggaatt tggtccactg atattttaaa ggaccagaaa      360

gaacccaaaa ataagacctt tctaagatgc gaggccaaga attattctgg acgtttcacc      420

tgctggtggc tgacgacaat cagtactgat ttgacattca gtgtcaaaag cagcagaggc      480

tcttctgacc cccaaggggt gacgtgcgga gctgctacac tctctgcaga gagagtcaga      540

ggggacaaca aggagtatga gtactcagtg gagtgccagg aggacagtgc ctgcccagct      600

gctgaggaga gtctgcccat tgaggtcatg gtggatgccg ttcacaagct caagtatgaa      660

aactacacca gcagcttctt catcagggac atcatcaaac ctgacccacc caagaacttg      720

cagctgaagc cattaaagaa ttctcggcag gtggaggtca gctgggagta ccctgacacc      780

tggagtactc cacattccta cttctccctg acattctgcg ttcaggtcca gggcaagagc      840

aagagagaaa agaaagatag agtcttcacg gacaagacct cagccacggt catctgccgc      900

aaaaatgcca gcattagcgt gcgggcccag gaccgctact atagctcatc ttggagcgaa      960

tgggcatctg tgccctgcag tggatctggg gccaccaact tttcattgct caagcaggcg     1020

ggcgatgtgg aggaaaaccc tggcccc                                         1047


<210>  7
<211>  861
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  [IgK signal peptide]-Flt3L-[GSSGSSG Hinge]-NY-ESO1(80-180aa)

<400>  7
gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac       60

actcaggatt gcagcttcca gcattcaccc atatcatcag attttgcagt aaagatcagg      120

gaactctccg attatctcct tcaagactac cccgtaacag tggcctccaa tttgcaagac      180

gaagagcttt gtggtgccct ctggcggctc gttttggccc aaaggtggat ggaacggctt      240

aagacagtcg ctggcagcaa gatgcaaggg ttgctcgaac gagtcaatac agagatccat      300

tttgtaacca agtgtgcatt tcaaccgccg ccaagctgcc ttcgctttgt tcagacgaat      360

ataagtagac tgttgcagga aacctccgag caactcgtag ccctgaagcc ctggattaca      420

cggcaaaatt tcagtcggtg ccttgagctt cagtgtcagc ctgatagtag taccttgcct      480

ccgccatggt cccccaggcc tcttgaagct acagctccga cagcccctca gccgggcagt      540

agtggtagtt ctggagccag ggggccggag agccgcctgc ttgagttcta cctcgccatg      600

cctttcgcga cacccatgga agcagagctg gcccgcagga gcctggccca ggatgcccca      660

ccgcttcccg tgccaggggt gcttctgaag gagttcactg tgtccggcaa catactgact      720

atccgactga ctgctgcaga ccaccgccaa ctgcagctct ccatcagctc ctgtctccag      780

cagctttccc tgttgatgtg gatcacgcag tgctttctgc ccgtgttttt ggctcagcct      840

ccctcagggc agaggcgcta a                                                861


<210>  8
<211>  1809
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hIL12p35-[GSG Hinge]-P2A-hIL12p40 nucleic acids

<400>  8
atgtggcccc ctgggtcagc ctcccagcca ccgccctcac ctgccgcggc cacaggtctg       60

catccagcgg ctcgccctgt gtccctgcag tgccggctca gcatgtgtcc agcgcgcagc      120

ctcctccttg tggctaccct ggtcctcctg gaccacctca gtttggccag aaacctcccc      180

gtggccactc cagacccagg aatgttccca tgccttcacc actcccaaaa cctgctgagg      240

gccgtcagca acatgctcca gaaggccaga caaactctag aattttaccc ttgcacttct      300

gaagagattg atcatgaaga tatcacaaaa gataaaacca gcacagtgga ggcctgttta      360

ccattggaat taaccaagaa tgagagttgc ctaaattcca gagagacctc tttcataact      420

aatgggagtt gcctggcctc cagaaagacc tcttttatga tggccctgtg ccttagtagt      480

atttatgaag acttgaagat gtaccaggtg gagttcaaga ccatgaatgc aaagcttctg      540

atggatccta agaggcagat ctttctagat caaaacatgc tggcagttat tgatgagctg      600

atgcaggccc tgaatttcaa cagtgagact gtgccacaaa aatcctccct tgaagaaccg      660

gatttttata aaactaaaat caagctctgc atacttcttc atgctttcag aattcgggca      720

gtgactattg atagagtgat gagctatctg aatgcttccg gatctggggc caccaacttt      780

tcattgctca agcaggcggg cgatgtggag gaaaaccctg gcccctgtca ccagcagttg      840

gtcatctctt ggttttccct ggtttttctg gcatctcccc tcgtggccat atgggaactg      900

aagaaagatg tttatgtcgt agaattggat tggtatccgg atgcccctgg agaaatggtg      960

gtcctcacct gtgacacccc tgaagaagat ggtatcacct ggaccttgga ccagagcagt     1020

gaggtcttag gctctggcaa aaccctgacc atccaagtca aagagtttgg agatgctggc     1080

cagtacacct gtcacaaagg aggcgaggtt ctaagccatt cgctcctgct gcttcacaaa     1140

aaggaagatg gaatttggtc cactgatatt ttaaaggacc agaaagaacc caaaaataag     1200

acctttctaa gatgcgaggc caagaattat tctggacgtt tcacctgctg gtggctgacg     1260

acaatcagta ctgatttgac attcagtgtc aaaagcagca gaggctcttc tgacccccaa     1320

ggggtgacgt gcggagctgc tacactctct gcagagagag tcagagggga caacaaggag     1380

tatgagtact cagtggagtg ccaggaggac agtgcctgcc cagctgctga ggagagtctg     1440

cccattgagg tcatggtgga tgccgttcac aagctcaagt atgaaaacta caccagcagc     1500

ttcttcatca gggacatcat caaacctgac ccacccaaga acttgcagct gaagccatta     1560

aagaattctc ggcaggtgga ggtcagctgg gagtaccctg acacctggag tactccacat     1620

tcctacttct ccctgacatt ctgcgttcag gtccagggca agagcaagag agaaaagaaa     1680

gatagagtct tcacggacaa gacctcagcc acggtcatct gccgcaaaaa tgccagcatt     1740

agcgtgcggg cccaggaccg ctactatagc tcatcttgga gcgaatgggc atctgtgccc     1800

tgcagttag                                                             1809


<210>  9
<211>  602
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIL12p35-[GSG-Hinge]-P2A-hIL12p40

<400>  9

Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly Pro Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val 
        275                 280                 285             


Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val 
    290                 295                 300                 


Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val 
305                 310                 315                 320 


Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu 
                325                 330                 335     


Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln 
            340                 345                 350         


Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly 
        355                 360                 365             


Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly 
    370                 375                 380                 


Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys 
385                 390                 395                 400 


Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys 
                405                 410                 415     


Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser 
            420                 425                 430         


Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr 
        435                 440                 445             


Leu Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser 
    450                 455                 460                 


Val Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu 
465                 470                 475                 480 


Pro Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn 
                485                 490                 495     


Tyr Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro 
            500                 505                 510         


Lys Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val 
        515                 520                 525             


Ser Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser 
    530                 535                 540                 


Leu Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys 
545                 550                 555                 560 


Asp Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys 
                565                 570                 575     


Asn Ala Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser 
            580                 585                 590         


Trp Ser Glu Trp Ala Ser Val Pro Cys Ser 
        595                 600         


<210>  10
<211>  2733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hIL12p35-P2A-p40-P2A-Flt3L-NY-ESO1

<400>  10
atgtggcccc ctgggtcagc ctcccagcca ccgccctcac ctgccgcggc cacaggtctg       60

catccagcgg ctcgccctgt gtccctgcag tgccggctca gcatgtgtcc agcgcgcagc      120

ctcctccttg tggctaccct ggtcctcctg gaccacctca gtttggccag aaacctcccc      180

gtggccactc cagacccagg aatgttccca tgccttcacc actcccaaaa cctgctgagg      240

gccgtcagca acatgctcca gaaggccaga caaactctag aattttaccc ttgcacttct      300

gaagagattg atcatgaaga tatcacaaaa gataaaacca gcacagtgga ggcctgttta      360

ccattggaat taaccaagaa tgagagttgc ctaaattcca gagagacctc tttcataact      420

aatgggagtt gcctggcctc cagaaagacc tcttttatga tggccctgtg ccttagtagt      480

atttatgaag acttgaagat gtaccaggtg gagttcaaga ccatgaatgc aaagcttctg      540

atggatccta agaggcagat ctttctagat caaaacatgc tggcagttat tgatgagctg      600

atgcaggccc tgaatttcaa cagtgagact gtgccacaaa aatcctccct tgaagaaccg      660

gatttttata aaactaaaat caagctctgc atacttcttc atgctttcag aattcgggca      720

gtgactattg atagagtgat gagctatctg aatgcttccg gatctggggc caccaacttt      780

tcattgctca agcaggcggg cgatgtggag gaaaaccctg gcccctgtca ccagcagttg      840

gtcatctctt ggttttccct ggtttttctg gcatctcccc tcgtggccat atgggaactg      900

aagaaagatg tttatgtcgt agaattggat tggtatccgg atgcccctgg agaaatggtg      960

gtcctcacct gtgacacccc tgaagaagat ggtatcacct ggaccttgga ccagagcagt     1020

gaggtcttag gctctggcaa aaccctgacc atccaagtca aagagtttgg agatgctggc     1080

cagtacacct gtcacaaagg aggcgaggtt ctaagccatt cgctcctgct gcttcacaaa     1140

aaggaagatg gaatttggtc cactgatatt ttaaaggacc agaaagaacc caaaaataag     1200

acctttctaa gatgcgaggc caagaattat tctggacgtt tcacctgctg gtggctgacg     1260

acaatcagta ctgatttgac attcagtgtc aaaagcagca gaggctcttc tgacccccaa     1320

ggggtgacgt gcggagctgc tacactctct gcagagagag tcagagggga caacaaggag     1380

tatgagtact cagtggagtg ccaggaggac agtgcctgcc cagctgctga ggagagtctg     1440

cccattgagg tcatggtgga tgccgttcac aagctcaagt atgaaaacta caccagcagc     1500

ttcttcatca gggacatcat caaacctgac ccacccaaga acttgcagct gaagccatta     1560

aagaattctc ggcaggtgga ggtcagctgg gagtaccctg acacctggag tactccacat     1620

tcctacttct ccctgacatt ctgcgttcag gtccagggca agagcaagag agaaaagaaa     1680

gatagagtct tcacggacaa gacctcagcc acggtcatct gccgcaaaaa tgccagcatt     1740

agcgtgcggg cccaggaccg ctactatagc tcatcttgga gcgaatgggc atctgtgccc     1800

tgcagtggat ctggggccac caacttttca ttgctcaagc aggcgggcga tgtggaggaa     1860

aaccctggcc ccgagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     1920

tccactggtg acactcagga ttgcagcttc cagcattcac ccatatcatc agattttgca     1980

gtaaagatca gggaactctc cgattatctc cttcaagact accccgtaac agtggcctcc     2040

aatttgcaag acgaagagct ttgtggtgcc ctctggcggc tcgttttggc ccaaaggtgg     2100

atggaacggc ttaagacagt cgctggcagc aagatgcaag ggttgctcga acgagtcaat     2160

acagagatcc attttgtaac caagtgtgca tttcaaccgc cgccaagctg ccttcgcttt     2220

gttcagacga atataagtag actgttgcag gaaacctccg agcaactcgt agccctgaag     2280

ccctggatta cacggcaaaa tttcagtcgg tgccttgagc ttcagtgtca gcctgatagt     2340

agtaccttgc ctccgccatg gtcccccagg cctcttgaag ctacagctcc gacagcccct     2400

cagccgggca gtagtggtag ttctggagcc agggggccgg agagccgcct gcttgagttc     2460

tacctcgcca tgcctttcgc gacacccatg gaagcagagc tggcccgcag gagcctggcc     2520

caggatgccc caccgcttcc cgtgccaggg gtgcttctga aggagttcac tgtgtccggc     2580

aacatactga ctatccgact gactgctgca gaccaccgcc aactgcagct ctccatcagc     2640

tcctgtctcc agcagctttc cctgttgatg tggatcacgc agtgctttct gcccgtgttt     2700

ttggctcagc ctccctcagg gcagaggcgc taa                                  2733


<210>  11
<211>  910
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hIL12p35-P2A-p40-P2A-Flt3L-NY-ESO1

<400>  11

Met Trp Pro Pro Gly Ser Ala Ser Gln Pro Pro Pro Ser Pro Ala Ala 
1               5                   10                  15      


Ala Thr Gly Leu His Pro Ala Ala Arg Pro Val Ser Leu Gln Cys Arg 
            20                  25                  30          


Leu Ser Met Cys Pro Ala Arg Ser Leu Leu Leu Val Ala Thr Leu Val 
        35                  40                  45              


Leu Leu Asp His Leu Ser Leu Ala Arg Asn Leu Pro Val Ala Thr Pro 
    50                  55                  60                  


Asp Pro Gly Met Phe Pro Cys Leu His His Ser Gln Asn Leu Leu Arg 
65                  70                  75                  80  


Ala Val Ser Asn Met Leu Gln Lys Ala Arg Gln Thr Leu Glu Phe Tyr 
                85                  90                  95      


Pro Cys Thr Ser Glu Glu Ile Asp His Glu Asp Ile Thr Lys Asp Lys 
            100                 105                 110         


Thr Ser Thr Val Glu Ala Cys Leu Pro Leu Glu Leu Thr Lys Asn Glu 
        115                 120                 125             


Ser Cys Leu Asn Ser Arg Glu Thr Ser Phe Ile Thr Asn Gly Ser Cys 
    130                 135                 140                 


Leu Ala Ser Arg Lys Thr Ser Phe Met Met Ala Leu Cys Leu Ser Ser 
145                 150                 155                 160 


Ile Tyr Glu Asp Leu Lys Met Tyr Gln Val Glu Phe Lys Thr Met Asn 
                165                 170                 175     


Ala Lys Leu Leu Met Asp Pro Lys Arg Gln Ile Phe Leu Asp Gln Asn 
            180                 185                 190         


Met Leu Ala Val Ile Asp Glu Leu Met Gln Ala Leu Asn Phe Asn Ser 
        195                 200                 205             


Glu Thr Val Pro Gln Lys Ser Ser Leu Glu Glu Pro Asp Phe Tyr Lys 
    210                 215                 220                 


Thr Lys Ile Lys Leu Cys Ile Leu Leu His Ala Phe Arg Ile Arg Ala 
225                 230                 235                 240 


Val Thr Ile Asp Arg Val Met Ser Tyr Leu Asn Ala Ser Gly Ser Gly 
                245                 250                 255     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            260                 265                 270         


Pro Gly Pro Cys His Gln Gln Leu Val Ile Ser Trp Phe Ser Leu Val 
        275                 280                 285             


Phe Leu Ala Ser Pro Leu Val Ala Ile Trp Glu Leu Lys Lys Asp Val 
    290                 295                 300                 


Tyr Val Val Glu Leu Asp Trp Tyr Pro Asp Ala Pro Gly Glu Met Val 
305                 310                 315                 320 


Val Leu Thr Cys Asp Thr Pro Glu Glu Asp Gly Ile Thr Trp Thr Leu 
                325                 330                 335     


Asp Gln Ser Ser Glu Val Leu Gly Ser Gly Lys Thr Leu Thr Ile Gln 
            340                 345                 350         


Val Lys Glu Phe Gly Asp Ala Gly Gln Tyr Thr Cys His Lys Gly Gly 
        355                 360                 365             


Glu Val Leu Ser His Ser Leu Leu Leu Leu His Lys Lys Glu Asp Gly 
    370                 375                 380                 


Ile Trp Ser Thr Asp Ile Leu Lys Asp Gln Lys Glu Pro Lys Asn Lys 
385                 390                 395                 400 


Thr Phe Leu Arg Cys Glu Ala Lys Asn Tyr Ser Gly Arg Phe Thr Cys 
                405                 410                 415     


Trp Trp Leu Thr Thr Ile Ser Thr Asp Leu Thr Phe Ser Val Lys Ser 
            420                 425                 430         


Ser Arg Gly Ser Ser Asp Pro Gln Gly Val Thr Cys Gly Ala Ala Thr 
        435                 440                 445             


Leu Ser Ala Glu Arg Val Arg Gly Asp Asn Lys Glu Tyr Glu Tyr Ser 
    450                 455                 460                 


Val Glu Cys Gln Glu Asp Ser Ala Cys Pro Ala Ala Glu Glu Ser Leu 
465                 470                 475                 480 


Pro Ile Glu Val Met Val Asp Ala Val His Lys Leu Lys Tyr Glu Asn 
                485                 490                 495     


Tyr Thr Ser Ser Phe Phe Ile Arg Asp Ile Ile Lys Pro Asp Pro Pro 
            500                 505                 510         


Lys Asn Leu Gln Leu Lys Pro Leu Lys Asn Ser Arg Gln Val Glu Val 
        515                 520                 525             


Ser Trp Glu Tyr Pro Asp Thr Trp Ser Thr Pro His Ser Tyr Phe Ser 
    530                 535                 540                 


Leu Thr Phe Cys Val Gln Val Gln Gly Lys Ser Lys Arg Glu Lys Lys 
545                 550                 555                 560 


Asp Arg Val Phe Thr Asp Lys Thr Ser Ala Thr Val Ile Cys Arg Lys 
                565                 570                 575     


Asn Ala Ser Ile Ser Val Arg Ala Gln Asp Arg Tyr Tyr Ser Ser Ser 
            580                 585                 590         


Trp Ser Glu Trp Ala Ser Val Pro Cys Ser Gly Ser Gly Ala Thr Asn 
        595                 600                 605             


Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
    610                 615                 620                 


Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro Gly 
625                 630                 635                 640 


Ser Thr Gly Asp Thr Gln Asp Cys Ser Phe Gln His Ser Pro Ile Ser 
                645                 650                 655     


Ser Asp Phe Ala Val Lys Ile Arg Glu Leu Ser Asp Tyr Leu Leu Gln 
            660                 665                 670         


Asp Tyr Pro Val Thr Val Ala Ser Asn Leu Gln Asp Glu Glu Leu Cys 
        675                 680                 685             


Gly Ala Leu Trp Arg Leu Val Leu Ala Gln Arg Trp Met Glu Arg Leu 
    690                 695                 700                 


Lys Thr Val Ala Gly Ser Lys Met Gln Gly Leu Leu Glu Arg Val Asn 
705                 710                 715                 720 


Thr Glu Ile His Phe Val Thr Lys Cys Ala Phe Gln Pro Pro Pro Ser 
                725                 730                 735     


Cys Leu Arg Phe Val Gln Thr Asn Ile Ser Arg Leu Leu Gln Glu Thr 
            740                 745                 750         


Ser Glu Gln Leu Val Ala Leu Lys Pro Trp Ile Thr Arg Gln Asn Phe 
        755                 760                 765             


Ser Arg Cys Leu Glu Leu Gln Cys Gln Pro Asp Ser Ser Thr Leu Pro 
    770                 775                 780                 


Pro Pro Trp Ser Pro Arg Pro Leu Glu Ala Thr Ala Pro Thr Ala Pro 
785                 790                 795                 800 


Gln Pro Gly Ser Ser Gly Ser Ser Gly Ala Arg Gly Pro Glu Ser Arg 
                805                 810                 815     


Leu Leu Glu Phe Tyr Leu Ala Met Pro Phe Ala Thr Pro Met Glu Ala 
            820                 825                 830         


Glu Leu Ala Arg Arg Ser Leu Ala Gln Asp Ala Pro Pro Leu Pro Val 
        835                 840                 845             


Pro Gly Val Leu Leu Lys Glu Phe Thr Val Ser Gly Asn Ile Leu Thr 
    850                 855                 860                 


Ile Arg Leu Thr Ala Ala Asp His Arg Gln Leu Gln Leu Ser Ile Ser 
865                 870                 875                 880 


Ser Cys Leu Gln Gln Leu Ser Leu Leu Met Trp Ile Thr Gln Cys Phe 
                885                 890                 895     


Leu Pro Val Phe Leu Ala Gln Pro Pro Ser Gly Gln Arg Arg 
            900                 905                 910 


<210>  12
<211>  3756
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV-hIL12p35-P2A-hIL12p40-Flt3L-NYESO-1

<400>  12
tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa       60

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata      120

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag      180

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc      240

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta      300

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg      360

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt      420

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca      480

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag      540

gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc      600

tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc      660

attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg      720

cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc      780

gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt      840

attgaccact ccaacggtgg agggcagtgt agtctgagca gtactcgttg ctgccgcgcg      900

cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg gtcttttctg      960

cagtcaccgt cgtcgacggt atcgataagc ttgatatcga attcacgtgg gcccggtacc     1020

accatgtggc cccctgggtc agcctcccag ccaccgccct cacctgccgc ggccacaggt     1080

ctgcatccag cggctcgccc tgtgtccctg cagtgccggc tcagcatgtg tccagcgcgc     1140

agcctcctcc ttgtggctac cctggtcctc ctggaccacc tcagtttggc cagaaacctc     1200

cccgtggcca ctccagaccc aggaatgttc ccatgccttc accactccca aaacctgctg     1260

agggccgtca gcaacatgct ccagaaggcc agacaaactc tagaatttta cccttgcact     1320

tctgaagaga ttgatcatga agatatcaca aaagataaaa ccagcacagt ggaggcctgt     1380

ttaccattgg aattaaccaa gaatgagagt tgcctaaatt ccagagagac ctctttcata     1440

actaatggga gttgcctggc ctccagaaag acctctttta tgatggccct gtgccttagt     1500

agtatttatg aagacttgaa gatgtaccag gtggagttca agaccatgaa tgcaaagctt     1560

ctgatggatc ctaagaggca gatctttcta gatcaaaaca tgctggcagt tattgatgag     1620

ctgatgcagg ccctgaattt caacagtgag actgtgccac aaaaatcctc ccttgaagaa     1680

ccggattttt ataaaactaa aatcaagctc tgcatacttc ttcatgcttt cagaattcgg     1740

gcagtgacta ttgatagagt gatgagctat ctgaatgctt ccggatctgg ggccaccaac     1800

ttttcattgc tcaagcaggc gggcgatgtg gaggaaaacc ctggcccctg tcaccagcag     1860

ttggtcatct cttggttttc cctggttttt ctggcatctc ccctcgtggc catatgggaa     1920

ctgaagaaag atgtttatgt cgtagaattg gattggtatc cggatgcccc tggagaaatg     1980

gtggtcctca cctgtgacac ccctgaagaa gatggtatca cctggacctt ggaccagagc     2040

agtgaggtct taggctctgg caaaaccctg accatccaag tcaaagagtt tggagatgct     2100

ggccagtaca cctgtcacaa aggaggcgag gttctaagcc attcgctcct gctgcttcac     2160

aaaaaggaag atggaatttg gtccactgat attttaaagg accagaaaga acccaaaaat     2220

aagacctttc taagatgcga ggccaagaat tattctggac gtttcacctg ctggtggctg     2280

acgacaatca gtactgattt gacattcagt gtcaaaagca gcagaggctc ttctgacccc     2340

caaggggtga cgtgcggagc tgctacactc tctgcagaga gagtcagagg ggacaacaag     2400

gagtatgagt actcagtgga gtgccaggag gacagtgcct gcccagctgc tgaggagagt     2460

ctgcccattg aggtcatggt ggatgccgtt cacaagctca agtatgaaaa ctacaccagc     2520

agcttcttca tcagggacat catcaaacct gacccaccca agaacttgca gctgaagcca     2580

ttaaagaatt ctcggcaggt ggaggtcagc tgggagtacc ctgacacctg gagtactcca     2640

cattcctact tctccctgac attctgcgtt caggtccagg gcaagagcaa gagagaaaag     2700

aaagatagag tcttcacgga caagacctca gccacggtca tctgccgcaa aaatgccagc     2760

attagcgtgc gggcccagga ccgctactat agctcatctt ggagcgaatg ggcatctgtg     2820

ccctgcagtg gatctggggc caccaacttt tcattgctca agcaggcggg cgatgtggag     2880

gaaaaccctg gccccgagac agacacactc ctgctatggg tactgctgct ctgggttcca     2940

ggttccactg gtgacactca ggattgcagc ttccagcatt cacccatatc atcagatttt     3000

gcagtaaaga tcagggaact ctccgattat ctccttcaag actaccccgt aacagtggcc     3060

tccaatttgc aagacgaaga gctttgtggt gccctctggc ggctcgtttt ggcccaaagg     3120

tggatggaac ggcttaagac agtcgctggc agcaagatgc aagggttgct cgaacgagtc     3180

aatacagaga tccattttgt aaccaagtgt gcatttcaac cgccgccaag ctgccttcgc     3240

tttgttcaga cgaatataag tagactgttg caggaaacct ccgagcaact cgtagccctg     3300

aagccctgga ttacacggca aaatttcagt cggtgccttg agcttcagtg tcagcctgat     3360

agtagtacct tgcctccgcc atggtccccc aggcctcttg aagctacagc tccgacagcc     3420

cctcagccgg gcagtagtgg tagttctgga gccagggggc cggagagccg cctgcttgag     3480

ttctacctcg ccatgccttt cgcgacaccc atggaagcag agctggcccg caggagcctg     3540

gcccaggatg ccccaccgct tcccgtgcca ggggtgcttc tgaaggagtt cactgtgtcc     3600

ggcaacatac tgactatccg actgactgct gcagaccacc gccaactgca gctctccatc     3660

agctcctgtc tccagcagct ttccctgttg atgtggatca cgcagtgctt tctgcccgtg     3720

tttttggctc agcctccctc agggcagagg cgctaa                               3756


<210>  13
<211>  5843
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression vector

<400>  13
tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg ctcatgtcca       60

acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg      120

tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg      180

cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata      240

gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc      300

cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac      360

ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg      420

cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc      480

aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc      540

aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc      600

gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct      660

cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga      720

agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc      780

cgtgccaaga gtgacgtaag taccgcctat agactctata ggcacacccc tttggctctt      840

atgcatgcta tactgttttt ggcttggggc ctatacaccc ccgcttcctt atgctatagg      900

tgatggtata gcttagccta taggtgtggg ttattgacca ttattgacca ctccaacggt      960

ggagggcagt gtagtctgag cagtactcgt tgctgccgcg cgcgccacca gacataatag     1020

ctgacagact aacagactgt tcctttccat gggtcttttc tgcagtcacc gtcgtcgacg     1080

gtatcgataa gcttgatatc gaattcacgt gggcccggta ccaccatgtg gccccctggg     1140

tcagcctccc agccaccgcc ctcacctgcc gcggccacag gtctgcatcc agcggctcgc     1200

cctgtgtccc tgcagtgccg gctcagcatg tgtccagcgc gcagcctcct ccttgtggct     1260

accctggtcc tcctggacca cctcagtttg gccagaaacc tccccgtggc cactccagac     1320

ccaggaatgt tcccatgcct tcaccactcc caaaacctgc tgagggccgt cagcaacatg     1380

ctccagaagg ccagacaaac tctagaattt tacccttgca cttctgaaga gattgatcat     1440

gaagatatca caaaagataa aaccagcaca gtggaggcct gtttaccatt ggaattaacc     1500

aagaatgaga gttgcctaaa ttccagagag acctctttca taactaatgg gagttgcctg     1560

gcctccagaa agacctcttt tatgatggcc ctgtgcctta gtagtattta tgaagacttg     1620

aagatgtacc aggtggagtt caagaccatg aatgcaaagc ttctgatgga tcctaagagg     1680

cagatctttc tagatcaaaa catgctggca gttattgatg agctgatgca ggccctgaat     1740

ttcaacagtg agactgtgcc acaaaaatcc tcccttgaag aaccggattt ttataaaact     1800

aaaatcaagc tctgcatact tcttcatgct ttcagaattc gggcagtgac tattgataga     1860

gtgatgagct atctgaatgc ttccggatct ggggccacca acttttcatt gctcaagcag     1920

gcgggcgatg tggaggaaaa ccctggcccc tgtcaccagc agttggtcat ctcttggttt     1980

tccctggttt ttctggcatc tcccctcgtg gccatatggg aactgaagaa agatgtttat     2040

gtcgtagaat tggattggta tccggatgcc cctggagaaa tggtggtcct cacctgtgac     2100

acccctgaag aagatggtat cacctggacc ttggaccaga gcagtgaggt cttaggctct     2160

ggcaaaaccc tgaccatcca agtcaaagag tttggagatg ctggccagta cacctgtcac     2220

aaaggaggcg aggttctaag ccattcgctc ctgctgcttc acaaaaagga agatggaatt     2280

tggtccactg atattttaaa ggaccagaaa gaacccaaaa ataagacctt tctaagatgc     2340

gaggccaaga attattctgg acgtttcacc tgctggtggc tgacgacaat cagtactgat     2400

ttgacattca gtgtcaaaag cagcagaggc tcttctgacc cccaaggggt gacgtgcgga     2460

gctgctacac tctctgcaga gagagtcaga ggggacaaca aggagtatga gtactcagtg     2520

gagtgccagg aggacagtgc ctgcccagct gctgaggaga gtctgcccat tgaggtcatg     2580

gtggatgccg ttcacaagct caagtatgaa aactacacca gcagcttctt catcagggac     2640

atcatcaaac ctgacccacc caagaacttg cagctgaagc cattaaagaa ttctcggcag     2700

gtggaggtca gctgggagta ccctgacacc tggagtactc cacattccta cttctccctg     2760

acattctgcg ttcaggtcca gggcaagagc aagagagaaa agaaagatag agtcttcacg     2820

gacaagacct cagccacggt catctgccgc aaaaatgcca gcattagcgt gcgggcccag     2880

gaccgctact atagctcatc ttggagcgaa tgggcatctg tgccctgcag ttagcgtata     2940

ctctagagcg gccgcggatc cagatctttt tccctctgcc aaaaattatg gggacatcat     3000

gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt     3060

gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa     3120

acatcagaat gagtatttgg tttagagttt ggcaacatat gcccattctt ccgcttcctc     3180

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     3240

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     3300

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     3360

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     3420

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     3480

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     3540

tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     3600

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     3660

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     3720

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     3780

cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     3840

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     3900

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     3960

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     4020

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     4080

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     4140

agcgatctgt ctatttcgtt catccatagt tgcctgactc gggggggggg ggcgctgagg     4200

tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag     4260

ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat     4320

tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc     4380

cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta     4440

atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc     4500

aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt     4560

ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat     4620

cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa     4680

ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa     4740

agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa     4800

tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg     4860

cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact     4920

gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct     4980

gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc     5040

ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta     5100

acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc     5160

ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac     5220

ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt     5280

tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt     5340

catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg     5400

ctttcccccc ccccccatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     5460

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     5520

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt     5580

atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg     5640

cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt     5700

cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag     5760

cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga     5820

aaataccgca tcagattggc tat                                             5843


<210>  14
<211>  2832
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-12 expression cassette

<400>  14
tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa       60

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata      120

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag      180

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc      240

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta      300

tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg      360

cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt      420

ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca      480

aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag      540

gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc      600

tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc      660

attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag actctatagg      720

cacacccctt tggctcttat gcatgctata ctgtttttgg cttggggcct atacaccccc      780

gcttccttat gctataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt      840

attgaccact ccaacggtgg agggcagtgt agtctgagca gtactcgttg ctgccgcgcg      900

cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg gtcttttctg      960

cagtcaccgt cgtcgacggt atcgataagc ttgatatcga attcacgtgg gcccggtacc     1020

accatgtggc cccctgggtc agcctcccag ccaccgccct cacctgccgc ggccacaggt     1080

ctgcatccag cggctcgccc tgtgtccctg cagtgccggc tcagcatgtg tccagcgcgc     1140

agcctcctcc ttgtggctac cctggtcctc ctggaccacc tcagtttggc cagaaacctc     1200

cccgtggcca ctccagaccc aggaatgttc ccatgccttc accactccca aaacctgctg     1260

agggccgtca gcaacatgct ccagaaggcc agacaaactc tagaatttta cccttgcact     1320

tctgaagaga ttgatcatga agatatcaca aaagataaaa ccagcacagt ggaggcctgt     1380

ttaccattgg aattaaccaa gaatgagagt tgcctaaatt ccagagagac ctctttcata     1440

actaatggga gttgcctggc ctccagaaag acctctttta tgatggccct gtgccttagt     1500

agtatttatg aagacttgaa gatgtaccag gtggagttca agaccatgaa tgcaaagctt     1560

ctgatggatc ctaagaggca gatctttcta gatcaaaaca tgctggcagt tattgatgag     1620

ctgatgcagg ccctgaattt caacagtgag actgtgccac aaaaatcctc ccttgaagaa     1680

ccggattttt ataaaactaa aatcaagctc tgcatacttc ttcatgcttt cagaattcgg     1740

gcagtgacta ttgatagagt gatgagctat ctgaatgctt ccggatctgg ggccaccaac     1800

ttttcattgc tcaagcaggc gggcgatgtg gaggaaaacc ctggcccctg tcaccagcag     1860

ttggtcatct cttggttttc cctggttttt ctggcatctc ccctcgtggc catatgggaa     1920

ctgaagaaag atgtttatgt cgtagaattg gattggtatc cggatgcccc tggagaaatg     1980

gtggtcctca cctgtgacac ccctgaagaa gatggtatca cctggacctt ggaccagagc     2040

agtgaggtct taggctctgg caaaaccctg accatccaag tcaaagagtt tggagatgct     2100

ggccagtaca cctgtcacaa aggaggcgag gttctaagcc attcgctcct gctgcttcac     2160

aaaaaggaag atggaatttg gtccactgat attttaaagg accagaaaga acccaaaaat     2220

aagacctttc taagatgcga ggccaagaat tattctggac gtttcacctg ctggtggctg     2280

acgacaatca gtactgattt gacattcagt gtcaaaagca gcagaggctc ttctgacccc     2340

caaggggtga cgtgcggagc tgctacactc tctgcagaga gagtcagagg ggacaacaag     2400

gagtatgagt actcagtgga gtgccaggag gacagtgcct gcccagctgc tgaggagagt     2460

ctgcccattg aggtcatggt ggatgccgtt cacaagctca agtatgaaaa ctacaccagc     2520

agcttcttca tcagggacat catcaaacct gacccaccca agaacttgca gctgaagcca     2580

ttaaagaatt ctcggcaggt ggaggtcagc tgggagtacc ctgacacctg gagtactcca     2640

cattcctact tctccctgac attctgcgtt caggtccagg gcaagagcaa gagagaaaag     2700

aaagatagag tcttcacgga caagacctca gccacggtca tctgccgcaa aaatgccagc     2760

attagcgtgc gggcccagga ccgctactat agctcatctt ggagcgaatg ggcatctgtg     2820

ccctgcagtt ag                                                         2832


