                         SEQUENCE LISTING

<110>  Aptamer Diagnostics Limited
 
<120>  Aptamer Against Irinotecan

<130>  P031428WO

<140>  GB1820631.8
<141>  2018-12-18

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  first randomised region (R1) of Aptamer CP13

<400>  1
gagaggattt                                                              10


<210>  2
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second randomised region (R2) of Aptamer CP13

<400>  2
cgctgtaagg ctcgggtatg agagtgtgga ggccagaggc                             40


<210>  3
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  best performing minimal effective nucleic acid fragment (F4c) of Aptamer CP13

<400>  3
tctccgagag gattttgagg ctcgatccgc tgtaaggctc gggtatg                     47


<210>  4
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid fragment (F4) of Aptamer CP13 with improved binding to Irinotecan as compared to full length CP13

<400>  4
agtccacgct ctttttctcc gagaggattt tgaggctcga tccgctgtaa ggctcgggta       60

tg                                                                      62


<210>  5
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid fragment (F4e) of Aptamer CP13 with improved binding to Irinotecan as compared to full length CP13

<400>  5
acgctctttt tctccgagag gattttgagg ctcgatccgc tgtaaggctc gggtatg          57


<210>  6
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid fragment (F5) of Aptamer CP13 with improved binding to Irinotecan as compared to full length CP13

<400>  6
agtccacgct ctttttctcc gagaggattt tgaggctcga tccgctgtaa ggctcgggta       60

tgagagt                                                                 67


<210>  7
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  full nucleic acid sequence of Aptamer CP13

<400>  7
agtccacgct ctttttctcc gagaggattt tgaggctcga tccgctgtaa ggctcgggta       60

tgagagtgtg gaggccagag gcgcattgag ggtgacatag g                          101


<210>  8
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  first randomised region (R1) of Aptamer CP11

<400>  8
tggtctttag a                                                            11


<210>  9
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  second randomised region (R2) of Aptamer CP11

<400>  9
cgctgtaagg ctcgggtatg agagtgtgga ggccagaggc                             40


<210>  10
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  best performing minimal effective nucleic acid fragment (F3e) of Aptamer CP11

<400>  10
cgctcttttt ctcctggtct ttagatgagg ctcgatcaat cctaaagagg ac               52


<210>  11
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleic acid fragment (F3) of Aptamer CP11 with improved binding to Irinotecan as compared to full length CP11

<400>  11
atccacgctc tttttctcct ggtctttaga tgaggctcga tcaatcctaa agaggac          57


<210>  12
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  full nucleic acid sequence of Aptamer CP11

<400>  12
atccacgctc tttttctcct ggtctttaga tgaggctcga tcaatcctaa agaggactag       60

atccgccagg gtaaaaggtg tgcattgagg gtgacatagg                            100


<210>  13
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary immobilisation region (I)

<400>  13
tgaggctcga tc                                                           12


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary first primer region (P1)

<400>  14
atccacgctc tttttctcc                                                    19


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary second primer region (P2)

<400>  15
gcattgaggg tgacatagg                                                    19


<210>  16
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary immobilisation sequence

<400>  16
gatcgagcct ca                                                           12


<210>  17
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse second primer region (P2)

<400>  17
cctatgtcac cctcaatgc                                                    19


