                         SEQUENCE LISTING

<110>  PTT Global Chemical Public Company Limited
 
<120>  MICROBIAL STRAINS ENGINEERED FOR IMPROVED FRUCTOSE UTILIZATION

<130>  524821WO

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  2660
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette for deleting the NEJ1 gene from Kluyveromyces marxianus.

<400>  1
gtacttgctg gcgtatatgt gatacacaaa ctaaacctat ggggtccttt ataccaattc       60

gctaatcaag caacgggaca agcaactatg actttgaaac agactgtccg taatttggtg      120

cttgatgaag accctaagcc tgtatccaca gagaaatact ctggagattt gaaggccagg      180

aaagaagatc cttctaaaga gagtacccat ttataaggag caaatataaa acaacaaaat      240

agcaatcgca accacatatt agataggtct gtagactata tccaatgcac caaaatatgg      300

catcctattt cctgtgcctt atcactacat gatcattcgt ttagatcttt taactaatat      360

tgtatataac cattttttaa ggctgtagtt cttacaaact taaccttaaa aaacttagca      420

tgggaacctt acaaagacta ctacgctgaa agaaatagta acctaagcgc tagattcgga      480

tttccttgca aaaaggatat gtatttctaa ccttttcttc tagctctccg atcaagctgg      540

acctaattca gagcaccttt gtagccaagt gtgaaagaac ttcgaagaag cctcagaatc      600

cacatctgca tcttccttac aaagcgcttg cacaatcttc gaccagaagg ttacaaatgc      660

cttcaattgg tgtacactaa ccggtacttc caccaattca ttaggcagtt gtttccaatc      720

attgtggcta ataccagaat ttctgaagaa atcagagaac accgcaatat aatgcttgta      780

aattaccctt gcgactgctt tgaagtctgt catttcttta cggctgtacg aagtgaacat      840

taactcgtca ccgtatatgc gtatcatagt acgatcaatg gcctccacaa tgacgttgaa      900

aaattcgtcg caggcgctaa cggcaagggc gtggaaatcc gaactggata cgccattgta      960

tgtaggctcg cgttcaattg gtttagggac ctggatattt tcaattcatc attttttttt     1020

tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag     1080

aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt     1140

gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg     1200

aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt     1260

cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca     1320

ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt     1380

tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag     1440

ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct     1500

gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg     1560

gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag     1620

gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc     1680

aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac     1740

aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac     1800

gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa     1860

cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga     1920

ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc     1980

tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat     2040

gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg     2100

aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaagctt     2160

tctaaagagc ttctgaaagc ctcaacgaaa gaagaaggac taatagatac actgcaggac     2220

atatacacaa tattatcaga agaatccaat tctaagtttg agtcattggg aagcaagatg     2280

tttctatcca tccaaagaaa agattcgtct gagattgtct tagaagtgga aacctccagt     2340

gttgatgaga gtcgagaaca aacgctacta catgagtgta ctggatattt gatgaaaaat     2400

gcggtggttg gaaatcatat tcgaacatat atttcccacc tcgcattaga aaaagatagg     2460

gcgatcgaat ttttagggaa tactgccaaa gattacggag ctagcaatac aatacttaaa     2520

tgggctcctg taaactctcc aaactacaaa tcattgatga aatttgatcc aagtttggca     2580

tgggcggaga gttttccaaa tggaagcgtc aatctagaca taatagatct taatgaatta     2640

aatgaaatta cacacaagag                                                 2660


<210>  2
<211>  5363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette for integrating and expressing ZrFFZ1 at the KmADH2 
       locus.

<400>  2
atgtctattc caactactca aaagggtgtt atcttctacg aaaacggtgg tcaattgtac       60

tacaaggaca tcccagtccc aaagccaaag tctaacgaac ttttgatcaa cgttaagtac      120

tccggtgtct gccacaccga tttgcacgcc tggaagggtg actggccatt ggacaccaag      180

ttgccattgg tcggtggtca cgaaggtgcc ggtgtcgtcg tcgccatggg tgacaacgtc      240

aagggctgga agatcggtga ccttgccggt atcaaatggt tgaacggttc ttgtatgaac      300

tgtgaagaat gtgaattgtc caacgaatcc aactgtccag acgctgactt gtccggttac      360

acccacgacg gttctttcca acaatacgct accgctgacg ctgtccaagc cgctcacatc      420

ccagctggta ccgacttggc tcaagtcgcc ccaatcttgt gtgccggtgt taccgtctac      480

aaggctttga agaccgctga ataaagcgtg aataatgaat ggccttgtat tcgttttttt      540

ccgagagaaa attaacaaga gcgaaaaaaa aaacgggctt cggtgaaaat cgggtgaata      600

tgcaactagc gggacgaatg ctctggaaat gcatatccta tgcaactagc gggatgaaca      660

aatctcaccc cagaattcgc aggaaaaaac aggaaaaaaa aaaagaaggc caccacggcc      720

acaaagacca caaagaccac aaaaaaaaac aaaaaacaac cgtcccagct tccagtgttt      780

ggaatactgg aacacaggaa gccgcataag agtgggcgtt gcacaggaag ccaggcccag      840

aagccccaga gttacttttt tttttttgtt ttttccttct gttcgctgtg cccgcatcag      900

atgatgcgcc tttatttacg atgccaatgc gaatagcacc agtgagagca ccagtaaaag      960

catacgcata cacatacaca catagagcaa gcaagcaggc tagcaaccag gaaaggctgc     1020

cagtgactgc tactgggtgt ctaagaaccg tagggcggat tattgttgcg gtggttggtt     1080

gcgggtggtt atgcgatggt acggtgcaga atcgtacggt gttgggttat ggaattagta     1140

tgggtatgtg atatgtggta atatgtgata ttgggttatt gtgatttgga atactgaata     1200

tcgaatatgg gatatggaat atggctatgg catggtatgg tatgggatgg gagtattcta     1260

ttttatttta ttctggttcc tgcgtttagg gtagggtagg aagaaggtga gtgcttttgt     1320

atataagtgg agtgtctgga tcagttttgt ggattgtgaa tgttagtttc ccctttaatg     1380

tatatttgta ttatttgctt ttgagtactc aataaccaag cacaactact agttttaaag     1440

gatccatcct cttaaacagt acaaatcgca aagaaaagct ccacacccaa accaaataat     1500

tgcaatggtc aaaatagacg ccagtgcaga tttggactgg aataatttgc ctccccaaca     1560

gaggttgggt agaatcgttg gtggaaaata tattgaaacc gtgcacatgg atgagtcatc     1620

accagaagcc atggagcgtc caatgacaaa agtcgagtgt gatatcccat taacttatac     1680

tgaatctgtg gaaaaggacg gcaaagaatg ggtaatgtta acttttgcac caggtgatcc     1740

agaaaaccca tttaattgga gtcttagatt gaaatggttt ctaacgactt tactgtgcct     1800

aatgacactt ttcatcggtc tagccaccac ggcttatagt tctggtatag acagaatggc     1860

aaaacacttt aacagttctt cggaagttgg tcagttgggt cttttcacct tcaatgaaac     1920

atgttccatt gcacctttgt ttctagcgcc attctgtgaa ttggttggta gaaaggtgat     1980

ttacaccggt ggttatcttt gcttctgtct ttgtttcatc ggtcttgcat taggtaaaaa     2040

tatggccact atcatttgtt tgcgtgcttg tttaggtttg tttggttgca ttggtaccat     2100

tcttgttggt ggtactttcg acgatatgtt tgttgcagat gaaagagcta ttcctatggc     2160

tctgtttgca tatgttgcta tcctgggtac cgtgggtgct ccaatctatg caggttttat     2220

tgatcaagcc attggctggc gttggattga aggtatccaa gggttatcta atgttccatt     2280

gttgattatc atctttttat ttttcaaaga aactcgtggt ggtgtcacct tgcaaaagag     2340

ggccaagtct ctacgtaaag ataccggtga tgagagatgg gtgtccaagg aagaattgga     2400

ggctcctggt cttaaggatg ctctttacaa ctcctctgtt aaggctatta agatgttaat     2460

ctccgagccc gtggtgtttt tctttggtct ttggatcagt ttcgcatggt tcctaacctt     2520

tttgtttttg tctgttatcg gtattacttt ctcacacttt aagcactgga gtgaaggtgt     2580

tgcaggttta ccttatatct cccttgttat tggtgttacc attggattct taaccaactt     2640

tttgcaaatt cgcaaatacg aaaatatgag aaagaagtca aagtatcctt taccacctga     2700

aaaccgttta tatggtgcta tgactggtgc cgtgttttta cctattggtc ttatgatcta     2760

ctctttcact caataccact ggttgcactg gatcggtcct aacatcggtt tggcatgcat     2820

ctctctcggt atcttcttca tcttcgaatc ctgttattcc tacacttcgg attgttatgg     2880

tcctgatgct tcttccgcca ttgcaggtca aggtttcatg cgtaatactt taggtgccgt     2940

ttcaccattg tttggtaagt acatgttctt aaacatgcac agtcaatggg ccggcctgtt     3000

gtgtagtctc gtggctttcg gtttgacatt gttgcccttc gtcctttaca aatttggtcc     3060

tgcaatccgt gccagatcta agcgtgctat tgtgtaccat ggtgaagaag acgatgaaga     3120

tgacgaaatt gctgaacagc aaagtagctc aagtactgga ggaggcagat ctttgcaaga     3180

tgatgctcac gttgaaaatg ctaacaacaa ccagttattc gaaaagaacg aaggttctcg     3240

tgaatctgat tctccagaga ctgctagaga gactgattcc ctatcacctt ccgaaaaggt     3300

agataaccat gctggagtat ctcgtagcgt ggaagaacac tcagataccg tttctgaaag     3360

tgaattacat ggccatactg gtcgtgcatg aaatatctat atcacaagat gaaaactgta     3420

ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta attatatcag     3480

ttattacccg ggaatctcgg tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga     3540

aagaaaaagc ttggatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg     3600

ctccgtccgg cgtagaggat cctcaattca tcattttttt tttattcttt tttttgattt     3660

cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag     3720

gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc     3780

ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg     3840

tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta     3900

tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc     3960

aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat     4020

gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc     4080

gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc     4140

aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca     4200

cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca     4260

aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact     4320

ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc     4380

tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca     4440

cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat     4500

gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga     4560

agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga     4620

agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca     4680

caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat     4740

ttttataatg acgaaaaaaa aaaaattgga aagaaaaagc ttggatccac aggacgggtg     4800

tggtcgccat gatcgcgtag tcgatagtgg ctcaacttag actaaggagg tttggggcgc     4860

gccatgaagg ctggtgactg ggtcgccatc tccggtgctg ctggtggtct aggttccttg     4920

gccgtccaat acgccaaggc catgggtttc agagtcctag gtatcgatgg tggtgaaggt     4980

aaggaagaat tgttcaagag cttgggtggt gaagtcttca ttgatttcac caagtctaag     5040

gacattgtcg gtgaagtcat caaggctacc aacggtggtg ctcacggtgt catcaacgtc     5100

tccgtctccg aaaaggccat cgaatcctcc atcgaatact gtagatccaa cggtaccgtc     5160

gttctagtcg gtttgccaaa ggacgccaag tgtaagtccg atgtcttcaa ccaagtcgtc     5220

aagtccatcc acattgttgg ttcttacgtc ggtaacagag ctgacaccag agaagctctt     5280

gacttcttct gcagaggtct agtccacgcc ccaatcaagg ttgtcggttt gtccaccttg     5340

ccagaaattt acgaaaagat gga                                             5363


<210>  3
<211>  6228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette from pJSS89 for integrating and expressing ZrFFZ1 by 
       insertion in the middle of the KmADH6 ORF.

<400>  3
cccggggatc ctctagagtc gagcggccgc cgaaagcatt gcgcttgtac ggtacactac       60

tgcatattcg gacgtggaaa cggcaagaaa aaaaagccag gaatgcaagt gcgggaataa      120

gctacgtacc gcacgctgta tacttgtgca ctagatgttt cttgtgcgct tcctatttgt      180

gcgtgaagac gtagagaaga ttatacaata caggtctcga gcgtgcgccg cgaatccagt      240

agattatgtc agaagagcaa atgggaagaa gcaaaagttg gtagggtaga gattatagat      300

gaggacgggt tttgatatga gagattagta attagtaatc acagagagag agagatggaa      360

tagaatgtct tagagacaca catgcacgtt tcacaatgtg taaaagtata aaaagaaggg      420

agcattgaat cttttcttgt caattgttta agctaatgta gttagtatga atttaaagag      480

cgtactacag taagctgata gatagaaggt atatccatac tttagccaaa taaagcgtga      540

ataatgaatg gccttgtatt cgtttttttc cgagagaaaa ttaacaagag cgaaaaaaaa      600

aacgggcttc ggtgaaaatc gggtgaatat gcaactagcg ggacgaatgc tctggaaatg      660

catatcctat gcaactagcg ggatgaacaa atctcacccc agaattcgca ggaaaaaaca      720

ggaaaaaaaa aaagaaggcc accacggcca caaagaccac aaagaccaca aaaaaaaaca      780

aaaaacaacc gtcccagctt ccagtgtttg gaatactgga acacaggaag ccgcataaga      840

gtgggcgttg cacaggaagc caggcccaga agccccagag ttactttttt ttttttgttt      900

tttccttctg ttcgctgtgc ccgcatcaga tgatgcgcct ttatttacga tgccaatgcg      960

aatagcacca gtgagagcac cagtaaaagc atacgcatac acatacacac atagagcaag     1020

caagcaggct agcaaccagg aaaggctgcc agtgactgct actgggtgtc taagaaccgt     1080

agggcggatt attgttgcgg tggttggttg cgggtggtta tgcgatggta cggtgcagaa     1140

tcgtacggtg ttgggttatg gaattagtat gggtatgtga tatgtggtaa tatgtgatat     1200

tgggttattg tgatttggaa tactgaatat cgaatatgga tatggaatat ggctatggca     1260

tggtatggta tgggatggga gtattctatt ttattttatt ctggttcctg cgtttagggt     1320

agggtaggaa gaaggtgagt gcttttgtat ataagtggag tgtctggatc agttttgtgg     1380

attgtgaatg ttagtttccc ctttaatgta tatttgtatt atttgctttt gagtactcaa     1440

taaccaagca caactactag ttttaaagga tccatcctct taaacagtac aaatcgcaaa     1500

gaaaagctcc acacccaaac caaataattg caatggtcaa aatagacgcc agtgcagatt     1560

tggactggaa taatttgcct ccccaacaga ggttgggtag aatcgttggt ggaaaatata     1620

ttgaaaccgt gcacatggat gagtcatcac cagaagccat ggagcgtcca atgacaaaag     1680

tcgagtgtga tatcccatta acttatactg aatctgtgga aaaggacggc aaagaatggg     1740

taatgttaac ttttgcacca ggtgatccag aaaacccatt taattggagt cttagattga     1800

aatggtttct aacgacttta ctgtgcctaa tgacactttt catcggtcta gccaccacgg     1860

cttatattct ggtatagaca gaatggcaaa acactttaac agttcttcgg aagttggtca     1920

gttgggtctt ttcaccttca atgaaacatg ttccattgca cctttgtttc tagcgccatt     1980

ctgtgaattg gttggtagaa aggtgattta caccggtggt tatctttgct tctgtctttg     2040

tttcatcggt cttgcattag gtaaaaatat ggccactatc atttgtttgc gtgcttgttt     2100

aggtttgttt ggttgcattg gtaccattct tgttggtggt actttcgacg atatgtttgt     2160

tgcagatgaa agagctattc ctatggctct gtttgcatat gttgctatcc tgggtaccgt     2220

gggtgctcca atctatgcag gttttattga tcaagccatt ggctggcgtt ggattgaagg     2280

tatccaaggg ttatctaatg ttccattgtt gattatcatc tttttatttt tcaaagaaac     2340

tcgtggtggt gtcaccttgc aaaagagggc caagtctcta cgtaaagata ccggtgatga     2400

gagatgggtg tccaaggaag aattggaggc tcctggtctt aaggatgctc tttacaactc     2460

ctctgttaag gctattaaga tgttaatctc cgagcccgtg gtgtttttct ttggtctttg     2520

gatcagtttc gcatggttcc taaccttttt gtttttgtct gttatcggta ttactttctc     2580

acactttaag cactggagtg aaggtgttgc aggtttacct tatatctccc ttgttattgg     2640

tgttaccatt ggattcttaa ccaacttttt gcaaattcgc aaatacgaaa atatgagaaa     2700

gaagtcaaag tatcctttac cacctgaaaa ccgtttatat ggtgctatga ctggtgccgt     2760

gtttttacct attggtctta tgatctactc tttcactcaa taccactggt tgcactggat     2820

cggtcctaac atcggtttgg catgcatctc tctcggtatc ttcttcatct tcgaatcctg     2880

ttattcctac acttcggatt gttatggtcc tgatgcttct tccgccattg caggtcaagg     2940

tttcatgcgt aatactttag gtgccgtttc accattgttt ggtaagtaca tgttcttaaa     3000

catgcacagt caatgggccg gcctgttgtg tagtctcgtg gctttcggtt tgacattgtt     3060

gcccttcgtc ctttacaaat ttggtcctgc aatccgtgcc agatctaagc gtgctattgt     3120

gtaccatggt gaagaagacg atgaagatga cgaaattgct gaacagcaaa gtagctcaag     3180

tactggagga ggcagatctt tgcaagatga tgctcacgtt gaaaatgcta acaacaacca     3240

gttattcgaa aagaacgaag gttctcgtga atctgattct ccagagactg ctagagagac     3300

tgattcccta tcaccttccg aaaaggtaga taaccatgct ggagtatctc gtagcgtgga     3360

agaacactca gataccgttt ctgaaagtga attacatggc catactggtc gtgcatgaac     3420

gttgtcattt tcatttcatt tcatttcata acatttctgt tttcatttgt ctttttttct     3480

gttattataa ttaacaaacg agaatggatc ttataaattc gtaacgatgg ccttcatacc     3540

acaattccat ctgaattact aacactatta actttatatt tttctttctg tctaacgaac     3600

ggtgtaaagc atatgataat aaattaacta tataggtttt aaaaaaagta tagaacgaac     3660

ttaaatgata tcaggataat tgaagatgtt tttttttact ttttgtttgc ttatatatta     3720

caaatataat gggaggggta cggagataaa actgtaagcg ggaacaatgg ttgtaaacgt     3780

caaacttcat gactccaaca agttagactt ttgcgtagcg tctgcacctg aaaatccggg     3840

gccgatacca acaagctaac actaacaaat actgtagaga cacggtacct agcaagttcc     3900

tagacacata aacagacaat ctagtacaca cggaagccag acaaccgcta caggcactac     3960

ttacgttatc tgcatttatc ctaatgaggc aaaaatctag aaaccatgga gactaccccg     4020

cgattaaagg cgattaaaag aaagtaccac aacgtagaac agagccgaac ttaaagtgga     4080

acaggtgtct acgagctgta cttcccgagc tggtttctcc aaacagccaa tgatcatacc     4140

aagatctgat gtatggcaac gttgttcacg tttgacatac gcagtatggt tgacaagaat     4200

cagggctctc aagctcagtt gagcggtttc cgggtcaaat tgctatgaat tcagtgatga     4260

gaattaatgc aactcccttc tttgtgacta gttcctacac tcaattgaaa cacggcatga     4320

gaattataaa actctaaagc aggtctaaac ttatactttg catgtcgttg catttccccc     4380

gtcttctaaa aaacataacc ctttcaatta tacttatata taatataata tgaattcgtc     4440

attctatgca gcattattat acagatccag atacaaatac gaagccattt tacaaaaaaa     4500

aaaaagatta tactacaaaa gttttgaagt agaatcgtaa gctttttctt tccaattttt     4560

tttttttcgt cattataaaa atcattacga ccgagattcc cgggtaataa ctgatataat     4620

taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt     4680

tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca     4740

ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca     4800

gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg     4860

tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc     4920

atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca     4980

gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc     5040

aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata     5100

cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca     5160

cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag     5220

agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa     5280

aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca     5340

actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc     5400

ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt     5460

tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc     5520

agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat     5580

accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca     5640

aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgacg     5700

tgttggtgtt ggtgcgcaag tcagatcctg tttggaatgt agacgttgta aggaggacaa     5760

cgaaccatac tgtccaaagt tcgtcactac ttactcgcaa ccatacccag aagatggtta     5820

cgtttcccaa ggtggttacg cctctcatat cagattgcat gagcactttg ccattccaat     5880

cccagaatct ctagaagccg cttacactgc tcctctactt tgcggtggtg gtactgtcta     5940

ctctccattg aagagatacg gctgtggtcc aggtaagaag gttggtatcg tcggtatcgg     6000

tggtattggt cacatgggta tccttttggc caaggctatg ggtgctgaag tttacgccat     6060

ctccagatct cacgctaaag aagaagctgc caagaaattg ggtgctgacc actacattgc     6120

taccaaggat gaaggctggg aattggaaaa ctttgacaaa ttggacttga tcgttctatg     6180

tgccagctcc ttgaccgagc ggccgcgccg ggtcacccgg ccagcgac                  6228


<210>  4
<211>  6094
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cassette from pJSS90 for integrating and expressing ZrFFZ1 with 
       simultaneous deletion of the KmADH6 ORF.

<400>  4
cccggggatc ctctagagtc gagcggccgc tttgatatga gagattagta attagtaatc       60

acagagagag agagatggaa tagaatgtct tagagacaca catgcacgtt tcacaatgtg      120

taaaagtata aaaagaaggg agcattgaat cttttcttgt caattgttta agctaatgta      180

gttagtatga atttaaagag cgtactacag taagctgata gatagaaggt atatccatac      240

tttagccaaa tagtcaaata atgtcctacc cagatagttt ccaaggtttc gctgtcgtta      300

atgaccacaa gaactggttg aacccagaaa agatcgttta cccagccaag aagttcggtc      360

ctcaagacgt tgatgtcgag atcgaagctt gcggtgtttg tggtagtgat gtccattgtg      420

ccaacggtaa ctggggtaat caaaagttgc cattagttgt tggtcacgaa gtcatcggta      480

aggttatcag agttggtgaa gaatgtacca ctggtatcaa ggttggtgac taaagcgtga      540

ataatgaatg gccttgtatt cgtttttttc cgagagaaaa ttaacaagag cgaaaaaaaa      600

aacgggcttc ggtgaaaatc gggtgaatat gcaactagcg ggacgaatgc tctggaaatg      660

catatcctat gcaactagcg ggatgaacaa atctcacccc agaattcgca ggaaaaaaca      720

ggaaaaaaaa aaagaaggcc accacggcca caaagaccac aaagaccaca aaaaaaaaca      780

aaaaacaacc gtcccagctt ccagtgtttg gaatactgga acacaggaag ccgcataaga      840

gtgggcgttg cacaggaagc caggcccaga agccccagag ttactttttt ttttttgttt      900

tttccttctg ttcgctgtgc ccgcatcaga tgatgcgcct ttatttacga tgccaatgcg      960

aatagcacca gtgagagcac cagtaaaagc atacgcatac acatacacac atagagcaag     1020

caagcaggct agcaaccagg aaaggctgcc agtgactgct actgggtgtc taagaaccgt     1080

agggcggatt attgttgcgg tggttggttg cgggtggtta tgcgatggta cggtgcagaa     1140

tcgtacggtg ttgggttatg gaattagtat gggtatgtga tatgtggtaa tatgtgatat     1200

tgggttattg tgatttggaa tactgaatat cgaatatggg atatggaata tggctatggc     1260

atggtatggt atgggatggg agtattctat tttattttat tcggttcctg cgtttagggt     1320

agggtaggaa gaaggtgagt gcttttgtat ataagtggag tgtctggatc agttttgtgg     1380

attgtgaatg ttagtttccc ctttaatgta tatttgtatt atttgctttt gagtactcaa     1440

taaccaagca caactactag ttttaaagga tccatcctct taaacagtac aaatcgcaaa     1500

gaaaagctcc acacccaaac caaataattg caatggtcaa aatagacgcc agtgcagatt     1560

tggactggaa taatttgcct ccccaacaga ggttgggtag aatcgttggt ggaaaatata     1620

ttgaaaccgt gcacatggat gagtcatcac cagaagccat ggagcgtcca atgacaaaag     1680

tcgagtgtga tatcccatta acttatactg aatctgtgga aaaggacggc aaagaatggg     1740

taatgttaac ttttgcacca ggtgatccag aaaacccatt taattggagt cttagattga     1800

aatggtttct aacgacttta ctgtgcctaa tgacactttt catcggtcta gccaccacgg     1860

cttatagttc tggtatagac agaatggcaa aacactttaa cagttcttcg gaagttggtc     1920

agttgggtct tttcaccttc aatgaaacat gttccattgc acctttgttt ctagcgccat     1980

tctgtgaatt ggttggtaga aaggtgattt acaccggtgg ttatctttgc ttctgtcttt     2040

gtttcatcgg tcttgcatta ggtaaaaata tggccactat catttgtttg cgtgcttgtt     2100

taggtttgtt tggttgcatt ggtaccattc ttgttggtgg tactttcgac gatatgtttg     2160

ttgcagatga aagagctatt cctatggctc tgtttgcata tgttgctatc ctgggtaccg     2220

tgggtgctcc aatctatgca ggttttattg atcaagccat tggctggcgt tggattgaag     2280

gtatccaagg gttatctaat gttccattgt tgattatcat ctttttattt ttcaaagaaa     2340

ctcgtggtgg tgtcaccttg caaaagaggg ccaagtctct acgtaaagat accggtgatg     2400

agagatgggt gtccaaggaa gaattggagg ctcctggtct taaggatgct ctttacaact     2460

cctctgttaa ggctattaag atgttaatct ccgagcccgt ggtgtttttc tttggtcttt     2520

ggatcagttt cgcatggttc ctaacctttt tgtttttgtc tgttatcggt attactttct     2580

cacactttaa gcactggagt gaaggtgttg caggtttacc ttatatctcc cttgttattg     2640

gtgttaccat tggattctta accaactttt tgcaaattcg caaatacgaa aatatgagaa     2700

agaagtcaaa gtatccttta ccacctgaaa accgtttata tggtgctatg actggtgccg     2760

tgtttttacc tattggtctt atgatctact ctttcactca ataccactgg ttgcactgga     2820

tcggtcctaa catcggtttg gcatgcatct ctctcggtat cttcttcatc ttcgaatcct     2880

gttattccta cacttcggat tgttatggtc ctgatgcttc ttccgccatt gcaggtcaag     2940

gtttcatgcg taatacttta ggtgccgttt caccattgtt tggtaagtac atgttcttaa     3000

acatgcacag tcaatgggcc ggcctgttgt gtagtctcgt ggctttcggt ttgacattgt     3060

tgcccttcgt cctttacaaa tttggtcctg caatccgtgc cagatctaag cgtgctattg     3120

tgtaccatgg tgaagaagac gatgaagatg acgaaattgc tgaacagcaa agtagctcaa     3180

gtactggagg aggcagatct ttgcaagatg atgctcacgt tgaaaatgct aacaacaacc     3240

agttattcga aaagaacgaa ggttctcgtg aatctgattc tccagagact gctagagaga     3300

ctgattccct atcaccttcc gaaaaggtag ataaccatgc tggagtatct cgtagcgtgg     3360

aagaacactc agataccgtt tctgaaagtg aattacatgg ccatactggt cgtgcatgaa     3420

cgttgtcatt ttcatttcat ttcatttcat aacatttctg ttttcatttg tctttttttc     3480

tgttattata attaacaaac gagaatggat cttataaatt cgtaacgatg gccttcatac     3540

cacaattcca tctgaattac taacactatt aactttatat ttttctttct gtctaacgaa     3600

cggtgtaaag catatgataa taaattcgtc cggcgtagag gatcctcaat tcatcatttt     3660

ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg gtaatctccg     3720

aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta     3780

gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct     3840

gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc     3900

ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca aacttgtgtg     3960

cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca ttaggtccca     4020

aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg gagggcacag     4080

ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgaa gacagaaaat     4140

ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac agaatagcag     4200

aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt agcggtttga     4260

agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta gcagaattgt     4320

catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac attgcgaaga     4380

gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga agagatgaag     4440

gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga gacgcattgg     4500

gtcaacagta tagaaccgtg gatgatgtgg tctctacagg atctgacatt attattgttg     4560

gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag     4620

caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaacgttg tcattttcat     4680

ttcatttcat ttcataacat ttctgttttc atttgtcttt ttttctgtta ttataattaa     4740

caaacgagaa tggatcttat aaattcgtaa cgatggcctt cataccacaa ttccatctga     4800

attactaaca ctattaactt tatatttttc tttctgtcta acgaacggtg taaagcatat     4860

gataataaat taactatata ggttttaaaa aaagtataga acgaacttaa atgatatcag     4920

gataattgaa gatgtttttt tttacttttt gtttgcttat atattacaaa tataatggga     4980

ggggtacgga gataaaactg taagcgggaa caatggttgt aaacgtcaaa cttcatgact     5040

ccaacaagtt agacttttgc gtagcgtctg cacctgaaaa tccggggccg ataccaacaa     5100

gctaacacta acaaatactg tagagacacg gtacctagca agttcctaga cacataaaca     5160

gacaatctag tacacacgga agccagacaa ccgctacagg cactacttac gttatctgca     5220

tttatcctaa tgaggcaaaa atctagaaac catggagact accccgcgat taaaggcgat     5280

taaaagaaag taccacaacg tagaacagag ccgaacttaa agtggaacag gtgtctacga     5340

gctgtacttc ccgagctggt ttctccaaac agccaatgat cataccaaga tctgatgtat     5400

ggcaacgttg ttcacgtttg acatacgcag tatggttgac aagaatcagg gctctcaagc     5460

tcagttgagc ggtttccggg tcaaattgct atgaattcag tgatgagaat taatgcaact     5520

cccttctttg tgactagttc ctacactcaa ttgaaacacg gcatcgtgtt ggtgttggtg     5580

cgcaagtcag atcctgtttg gaatgtagac gttgtaagga ggacaacgaa ccatactgtc     5640

caaagttcgt cactacttac tcgcaaccat acccagaaga tggttacgtt tcccaaggtg     5700

gttacgcctc tcatatcaga ttgcatgagc actttgccat tccaatccca gaatctctag     5760

aagccgctta cactgctcct ctactttgcg gtggtggtac tgtctactct ccattgaaga     5820

gatacggctg tggtccaggt aagaaggttg gtatcgtcgg tatcggtggt attggtcaca     5880

tgggtatcct tttggccaag gctatgggtg ctgaagttta cgccatctcc agatctcacg     5940

ctaaagaaga agctgccaag aaattgggtg ctgaccacta cattgctacc aaggatgaag     6000

gctgggaatt ggaaaacttt gacaaattgg acttgatcgt tctatgtgcc agctccttga     6060

ccgagcggcc gcgccgggtc acccggccag cgac                                 6094


<210>  5
<211>  6398
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pMS155 containing the integration cassette for swapping 
       in the PaldhL expression cassette to replace the EcldhA 
       expression cassette at any of the three loci.

<400>  5
agcatacgca tacacataca cacatagagc aagcaagcag gctagcaacc aggaaaggct       60

gccagtgact gctactgggt gtctaagaac cgtagggcgg attattgttg cggtggttgg      120

ttgcgggtgg ttatgcgatg gtacggtgca gaatcgtacg gtgttgggtt atggaattag      180

tatgggtatg tgatatgtgg taatatgtga tattgggtta ttgtgatttg gaatactgaa      240

tatcgaatat gggatatgga atatggctat ggcatggtat ggtatgggat gggagtattc      300

tattttattt tattctggtt cctgcgttta gggtagggta ggaagaaggt gagtgctttt      360

gtatataagt ggagtgtctg gatcagtttt gtggattgtg aatgttagtt tcccctttaa      420

tgtatatttg tattatttgc ttttgagtac tcaataccaa gcacaactac tagttttaaa      480

ggatccatcc tcttaaacag tacaaatcgc aaagaaaagc tccacaccca aaccaaataa      540

ttgcaatgtc taatattcaa aatcatcaaa aagttgtcct cgtcggtgac ggtgccgtag      600

gttctagtta cgcattcgcg atggcactac aaggaatcgc tgaagaattc gtcattgtcg      660

acgttgttaa ggatcgtaca gttggggacg cattggacct tgaagatgct actccattca      720

cagctccaaa gaacatctac tctggtgaat actcagactg caaggatgct gacttagttg      780

ttatcacagc tggcgcacca caaaagccag gtgaaacacg tcttgacctt gttaacaaga      840

acttaaacat cctttcaacg attgttaaac cagttgttga ttctggtttt gatggtatct      900

tccttgttgc tgctaaccca gttgatatcc ttacttacgc aacatggaaa ttctctggct      960

tccctaagga aaaagttatc ggttcaggta tctcacttga cacagctcgt ttgcgcgtag     1020

ctcttggtaa gaaattcaac gttagcccag aatctgtaga tgcttacatc ttaggtgaac     1080

atggtgacag tgaatttgct gcttactcat cagctacaat cggtacaaag ccattgcttg     1140

aaatcgctaa agaagaaggc gtttcaactg acgaattggc tgaaatcgaa gacagcgtac     1200

gtaacgcagc ttacgaaatc atcaacaaga agggtgctac attctacggt gttggtactg     1260

cattgatgcg catttctaaa gcaattcttc gcgacgaaaa cgccgtattg cctgttggtg     1320

catacatgga tggcgaatat ggtttgaacg acatttacat tggtactcct gcagttatca     1380

atggtcaagg tctaaaccgc gttatcgaag caccacttag cgatgacgaa aagaagaaga     1440

tgactgactc agcaactact ttgaagaagg ttcttactga cggtctaaac gctcttgctg     1500

aaaaacaaga caaataaggc gcgggagatt gataagactt ttctagttgc atatctttta     1560

tatttaaatc ttatctatta gttaattttt tgtaatttat ccttatatat agtctggtta     1620

ttctaaaata tcatttcagt atctaaaaat tcccctcttt tttcagttat atcttaacag     1680

gcgacagtcc aaatgttgat ttatcccagt ccgattcatc agggttgtga agccaatact     1740

gccatttcaa agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca     1800

aaaaattagc cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac     1860

acagaatata taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat     1920

ataatggagc ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat     1980

attgttttct tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa     2040

caggggcaca aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg     2100

agtaaatgat gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct     2160

tctattacct tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt     2220

ccctgaaatt attcccctac ttgactaata agtatataaa gacggtaggt attgattgta     2280

attctgtaaa tctatttctt aaacttctta aattctactt ttatagttag tctttttttt     2340

agttttaaaa caccaagaac ttagtttcga ataaacacac ataaacaaac aaaatgtcga     2400

ctaagagtta ctcggaaaga gcagctgctc atagaagtcc agttgctgcc aagcttttaa     2460

acttgatgga agagaagaag tcaaacttat gtgcttctct tgatgttcgt aaaacagcag     2520

agttgttaaa attagtcgag gttttgggtc catatatctg tctattgaag acacatgtag     2580

atatcttgga ggatttcagc tttgagaata ccattgtgcc gttgaagcaa ttagcagaga     2640

aacacaagtt tttgatattt gaagacagga agtttgccga cattgggaac actgttaaat     2700

tacaatacac gtctggtgta taccgtatcg ccgaatggtc tgatatcacc aatgcacacg     2760

gtgtgactgg tgcgggcatt gttgctggtt tgaagcaagg tgccgaggaa gttacgaaag     2820

aacctagagg gttgttaatg cttgccgagt tatcgtccaa ggggtctcta gcgcacggtg     2880

aatacactcg tgggaccgtg gaaattgcta agagtgataa ggactttgtt attggattta     2940

ttgctcaaaa cgatatgggt ggaagagaag agggctacga ttggttgatc atgacgccag     3000

gtgttggtct tgatgacaaa ggtgatgctt tgggacaaca atacagaact gtggatgaag     3060

ttgttgccgg tggatcagac atcattattg ttggtagagg tcttttcgca aagggaagag     3120

atcctgtagt ggaaggtgag agatacagaa aggcgggatg ggacgcttac ttgaagagag     3180

taggcagatc cgcttaatca tgtaattagt tatgtcacgc ttacattcac gccctccccc     3240

cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta     3300

tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt     3360

tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt     3420

gggacgctcg aaggctttaa tttgcgtgat gctaacttct ctctggaagg tctgaccggc     3480

tttactatgt atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg     3540

ctgcgcattc tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca     3600

gcggcgctgg aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac     3660

gttatctctc tgcactgccc gctgacaccg gaaaactatc atctgttgaa cgaagccgcc     3720

ttcgaacaga tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat     3780

tctcaggcag caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtg     3840

tatgagaacg aacgcgatct attctttgaa gataaatcca acgacgtgat ccaggatgac     3900

gtattccgtc gcctgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg     3960

acagcagaag ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa     4020

aaaggcgaaa cctgcccgaa gaactggtct accctgtgga acacctacat ctgtattaac     4080

gaagcgctaa ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt     4140

attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc     4200

acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa     4260

cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc     4320

gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta     4380

aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct     4440

gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac     4500

cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat     4560

attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa     4620

catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc     4680

ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga     4740

aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac     4800

cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag     4860

aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc     4920

cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc     4980

aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt     5040

ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag     5100

tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt     5160

tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc     5220

tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc     5280

caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta     5340

tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg     5400

gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag     5460

tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga     5520

tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg     5580

cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt     5640

aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca     5700

agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat     5760

accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta     5820

ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc     5880

gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc     5940

tgcgccttat ccggtaacta tcgtcttgag tccacccgga aagacatgca aaagcaccac     6000

tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa     6060

ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc     6120

aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt     6180

tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag     6240

ataaaatatt tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc     6300

cccatacgat ataagttgta attctcatgt ttgacagctt atcatcgata agctttaatg     6360

cggtagttta tcacagttaa attgctaacg cagtcagg                             6398


<210>  6
<211>  8515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pRY789 containing a ZrFFZ1 expression cassette for 
       integration at the HO locus of Saccharomyces cerevisiae.

<400>  6
tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga       60

tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga      120

ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa      180

ttgtaatacg actcactata gggcgaattg ggcccgacgt cgcatgctcc cggccgccat      240

ggccgcggac agcatcaaac tgtaagattc cgccacattt tatacactct ggtcctttaa      300

ctggcaaacc ttcgggcgta atgcccaatt tttcgccttt gtcttttgcc tttttcactt      360

cacgtgcttc tggtacatac ttgcaattta tacagtgatg accgctgaat ttgtatcttc      420

catagcatct agcacatact cgatttttac cactccaatc tttataaaaa tacttgattc      480

cctttctggg acaagcaaca cagtgtttta gattcttttt ttgtgatatt ttaagctgtt      540

ctcccacaca gcagcctcga catgatttca cttctatttt gttgccaagc aagaaatttt      600

tatggccttc tatcgtaagc ccatatacag tactctcacc ctggaaatca tccgtgaagc      660

tgaaatatac gggttccctt tttataattg gcggaacttc tcttgttttg tgaccacttc      720

gacaatatga caaaacattc tgtgaagttg ttcccccagc aacattacag tcgtatgtaa      780

attgacattg gacttttctt ccttcaatga tttcctccct agctgacctg gtcgtcacag      840

tagctgacat accaagagat cttgaaatat ggacaattcc gtccataatg gatgaataaa      900

cagtttgaat tgctatttta taagattcag ggccttcgcc ctttttcaca acgtacccat      960

ctgagtcgat caagccggct aagaatgctt cacgaacttc tatatgctcg ccgtacataa     1020

attcagggat ttgcttctct ccatcaagat cccttttaaa ctttaaaatt gtgacagctt     1080

tccagaatgg attatttttc ctcaaattcc ttgtcttcct gttttcatct ggaccatctc     1140

cataatgaag ccttacatgt ttggcacgta gcggaacgtg atcgtcacaa accgtaaggt     1200

agagacccca gattttcgca ttttctctta aactctccat tagcttagga tccaagctat     1260

ctactgagat ttctggctct tttgttgtac tgtcacctaa ccacagaccc agcgtgaata     1320

atgaatggcc ttgtattcgt ttttttccga gagaaaatta acaagagcga aaaaaaaaac     1380

gggcttcggt gaaaatcggg tgaatatgca actagcggga cgaatgctct ggaaatgcat     1440

atcctatgca actagcggga tgaacaaatc tcaccccaga attcgcagga aaaaacagga     1500

aaaaaaaaaa gaaggccacc acggccacaa agaccacaaa gaccacaaaa aaaaacaaaa     1560

aacaaccgtc ccagcttcca gtgtttggaa tactggaaca caggaagccg cataagagtg     1620

ggcgttgcac aggaagccag gcccagaagc cccagagtta cttttttttt tttgtttttt     1680

ccttctgttc gctgtgcccg catcagatga tgcgccttta tttacgatgc caatgcgaat     1740

agcaccagtg agagcaccag taaaagcata cgcatacaca tacacacata gagcaagcaa     1800

gcaggctagc aaccaggaaa ggctgccagt gactgctact gggtgtctaa gaaccgtagg     1860

gcggattatt gttgcggtgg ttggttgcgg gtggttatgc gatggtacgg tgcagaatcg     1920

tacggtgttg ggttatggaa ttagtatggg tatgtgatat gtggtaatat gtgatattgg     1980

gttattgtga tttggaatac tgaatatcga atatgggata tggaatatgg ctatggcatg     2040

gtatggtatg ggatgggagt attctatttt attttattct ggttcctgcg tttagggtag     2100

ggtaggaaga aggtgagtgc ttttgtatat aagtggagtg tctggatcag ttttgtggat     2160

tgtgaatgtt agtttcccct ttaatgtata tttgtattat ttgcttttga gtactcaata     2220

accaagcaca actactagtt ttaaaggatc catcctctta aacagtacaa atcgcaaaga     2280

aaagctccac acccaaacca aataattgca atggtcaaaa tagacgccag tgcagatttg     2340

gactggaata atttgcctcc ccaacagagg ttgggtagaa tcgttggtgg aaaatatatt     2400

gaaaccgtgc acatggatga gtcatcacca gaagccatgg agcgtccaat gacaaaagtc     2460

gagtgtgata tcccattaac ttatactgaa tctgtggaaa aggacggcaa agaatgggta     2520

atgttaactt ttgcaccagg tgatccagaa aacccattta attggagtct tagattgaaa     2580

tggtttctaa cgactttact gtgcctaatg acacttttca tcggtctagc caccacggct     2640

tatagttctg gtatagacag aatggcaaaa cactttaaca gttcttcgga agttggtcag     2700

ttgggtcttt tcaccttcaa tgaaacatgt tccattgcac ctttgtttct agcgccattc     2760

tgtgaattgg ttggtagaaa ggtgatttac accggtggtt atctttgctt ctgtctttgt     2820

ttcatcggtc ttgcattagg taaaaatatg gccactatca tttgtttgcg tgcttgttta     2880

ggtttgtttg gttgcattgg taccattctt gttggtggta ctttcgacga tatgtttgtt     2940

gcagatgaaa gagctattcc tatggctctg tttgcatatg ttgctatcct gggtaccgtg     3000

ggtgctccaa tctatgcagg ttttattgat caagccattg gctggcgttg gattgaaggt     3060

atccaagggt tatctaatgt tccattgttg attatcatct ttttattttt caaagaaact     3120

cgtggtggtg tcaccttgca aaagagggcc aagtctctac gtaaagatac cggtgatgag     3180

agatgggtgt ccaaggaaga attggaggct cctggtctta aggatgctct ttacaactcc     3240

tctgttaagg ctattaagat gttaatctcc gagcccgtgg tgtttttctt tggtctttgg     3300

atcagtttcg catggttcct aacctttttg tttttgtctg ttatcggtat tactttctca     3360

cactttaagc actggagtga aggtgttgca ggtttacctt atatctccct tgttattggt     3420

gttaccattg gattcttaac caactttttg caaattcgca aatacgaaaa tatgagaaag     3480

aagtcaaagt atcctttacc acctgaaaac cgtttatatg gtgctatgac tggtgccgtg     3540

tttttaccta ttggtcttat gatctactct ttcactcaat accactggtt gcactggatc     3600

ggtcctaaca tcggtttggc atgcatctct ctcggtatct tcttcatctt cgaatcctgt     3660

tattcctaca cttcggattg ttatggtcct gatgcttctt ccgccattgc aggtcaaggt     3720

ttcatgcgta atactttagg tgccgtttca ccattgtttg gtaagtacat gttcttaaac     3780

atgcacagtc aatgggccgg cctgttgtgt agtctcgtgg ctttcggttt gacattgttg     3840

cccttcgtcc tttacaaatt tggtcctgca atccgtgcca gatctaagcg tgctattgtg     3900

taccatggtg aagaagacga tgaagatgac gaaattgctg aacagcaaag tagctcaagt     3960

actggaggag gcagatcttt gcaagatgat gctcacgttg aaaatgctaa caacaaccag     4020

ttattcgaaa agaacgaagg ttctcgtgaa tctgattctc cagagactgc tagagagact     4080

gattccctat caccttccga aaaggtagat aaccatgctg gagtatctcg tagcgtggaa     4140

gaacactcag ataccgtttc tgaaagtgaa ttacatggcc atactggtcg tgcatgaaat     4200

atctatatca caagatggac tcaatgttga gcaaagcagg acgagaaaaa aaaaaataat     4260

gattgttaag aagttcatga aaaaaaaaag aaaaaatact caaatactta taacagagtg     4320

attaaataat aaacgacagt ataccctatc aggtattcag atagttttat ttttgtaggt     4380

atataatctg aagcctttga actattttct cgtatatatc atggagtata cattgcatta     4440

gcaacattac atactagtga cttttgagaa gtggcttttg gtttctcaat tttttgaagg     4500

aaacatcctt ggacctgtgg caactcctcc aataattctt cgaagttatg ttgaactttt     4560

cgtggtttca cacctaataa cgcccagcgg tagtacaatt caaagtagta ggtaccaatg     4620

gtagtactag tgggtgatag tagcaaattt ctctatgaaa atgaattgtt gctctgcaat     4680

gatgttaatg tggacatgac atgaattttc gcgatatgtt gatgtaaata ataacatgat     4740

tttcgtggat catttcgtaa accataggtt tatttcgtga attactaaga gaaaggcata     4800

gaaacaggac ttgcgaaccc tttctgtaat atgtcataat atttcaatac acgcacaaaa     4860

aaggtacgtt aatttccatg gtaatcgtgt atttagttac atcacttttc gtgacacaca     4920

tgaatacatt tgcccttaag ccctgtgtag gattgatttc ttttttcgtg acgcacatgt     4980

cttcaactgc atcaatttcc atgagacaca cacgtctacc atgttgtcag atctcatgaa     5040

tctttcatgt attcattcac atcattttcg tggatcctct aattgtatcg agatcacttt     5100

tcgtgatccg ctaatcagcg acggtcacat taggtttgcc aagtcagggt atgaaccata     5160

cgatcagttt tcgtgaacct ggtacgtata ttgtggcgtt tgtgtatatt ttcattcttt     5220

gacaacaatc aataccaacc tcaaatagga aaagtaataa gtttggcgtt acaccccaaa     5280

agacgccaaa cggatcgaac ttactcaata gcaattagcg agacaaaacc tacgttaaga     5340

cctgtaaccg atttatcaaa gcactctgcg gttctttctt gggaatatta cctggacatt     5400

ttgtgccctc aagaaacgag gctctacgag cctgttggag cccctcagac attagccgcc     5460

acgaatcaaa ctttttacgc gattcggccc aaatcagttt ctcacagatc attcgtagag     5520

tgaaaaagca catcgattat ttgatacccc tttgggttaa ttactgttga ggtcttttct     5580

atttctgatt gaaatttttg ctccagcatt atagcatgct ggtttttttt ttaaattgat     5640

gtatctcatc gcaggcacgg gcagtacagt gccctgagcg tagggaaaaa tgaaaaaaag     5700

gatgtaactt ttaacataat tccagcacgc agcgattggg tataatgaag attgttaagt     5760

tcaacaacat gtgaaattct tagctccgca acttgcttga attatgagct cccaacgcgt     5820

tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca     5880

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga     5940

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg     6000

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc     6060

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac     6120

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     6180

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     6240

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     6300

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     6360

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     6420

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     6480

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     6540

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     6600

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     6660

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga     6720

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     6780

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     6840

attacgcgca gaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg     6900

ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct     6960

tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     7020

aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     7080

tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg     7140

gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     7200

atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     7260

tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     7320

ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt     7380

ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca     7440

tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg     7500

ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat     7560

ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta     7620

tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca     7680

gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct     7740

taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat     7800

cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa     7860

agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt     7920

gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa     7980

ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgat gcggtgtgaa     8040

ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt     8100

tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     8160

tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     8220

tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     8280

tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     8340

ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     8400

gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     8460

cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaa          8515


<210>  7
<211>  8458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid pBc-ldhL-int containing the integration cassette for 
       swapping in the BcldhL expression cassette to replace the EcldhA 
       expression cassette at any of the three loci.

<400>  7
atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg       60

gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac      120

cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac      180

atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga      240

actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat      300

gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt      360

ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa      420

tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag      480

gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac      540

catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact      600

tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc      660

caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa      720

atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctacg taacggacta      780

agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa      840

tttttgagtg acatgcaaag taagcatgat ctcaatggtt cgttctcatg gctcacgcaa      900

aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat      960

ggcagcgtga ataatgaatg gccttgtatt cgtttttttc cgagagaaaa ttaacaagag     1020

cgaaaaaaaa aacgggcttc ggtgaaaatc gggtgaatat gcaactagcg ggacgaatgc     1080

tctggaaatg catatcctat gcaactagcg ggatgaacaa atctcacccc agaattcgca     1140

ggaaaaaaca ggaaaaaaaa aaagaaggcc accacggcca caaagaccac aaagaccaca     1200

aaaaaaaaca aaaaacaacc gtcccagctt ccagtgtttg gaatactgga acacaggaag     1260

ccgcataaga gtgggcgttg cacaggaagc caggcccaga agccccagag ttactttttt     1320

ttttttgttt tttccttctg ttcgctgtgc ccgcatcaga tgatgcgcct ttatttacga     1380

tgccaatgcg aatagcacca gtgagagcac cagtaaaagc atacgcatac acatacacac     1440

atagagcaag caagcaggct agcaaccagg aaaggctgcc agtgactgct actgggtgtc     1500

taagaaccgt agggcggatt attgttgcgg tggttggttg cgggtggtta tgcgatggta     1560

cggtgcagaa tcgtacggtg ttgggttatg gaattagtat gggtatgtga tatgtggtaa     1620

tatgtgatat tgggttattg tgatttggaa tactgaatat cgaatatggg atatggaata     1680

tggctatggc atggtatggt atgggatggg agtattctat tttattttat tctggttcct     1740

gcgtttaggg tagggtagga agaaggtgag tgcttttgta tataagtgga gtgtctggat     1800

cagttttgtg gattgtgaat gttagtttcc cctttaatgt atatttgtat tatttgcttt     1860

tgagtactca ataaccaagc acaactacta gttttaaagg atccatcctc ttaaacagta     1920

caaatcgcaa agaaaagctc cacacccaaa ccaaataatt gcaatgaaga aggtgaatcg     1980

tattgccgtg gtcggcactg gcgcagttgg tacgagttac tgctacgcta tgataaatca     2040

gggggttgcc gaggagttgg tgttgataga tattaatgag gctaaggccg aaggggaagc     2100

aatggatttg aatcacggtc ttccatttgc tccgactccc accagagttt ggaagggcga     2160

ctacagtgac tgtggtaccg cagacctagt agtaatcacc gccgggtccc cccagaagcc     2220

cggagaaacc cgtcttgact tggtttctaa gaacgcaaaa atctttaaag gcatgattaa     2280

atcaataatg gacagtggtt tcaacggcat tttcttggta gcatcaaacc ccgttgatat     2340

tcttacatac gttacgtgga aagaatcagg gttgccgaag gagcacgtga taggttcagg     2400

aacagttctt gactcagccc gtctaagaaa ctcactttcc gcccagtttg gaattgaccc     2460

aagaaatgtc cacgctgcca ttataggtga acacggggac acggagttac ccgtctggtc     2520

tcatactaac attggctacg acacgattga gtcgtactta caaaaaggta tcatagacga     2580

aaagacgctt gatgatatct ttgtcaatac tagagacgct gcataccaca tcatcgaaag     2640

gaagggagcc acattctatg gaataggcat gtcgttgact aggatcacca gggccatttt     2700

gaacaatgag aatagtgttt taacggttag tgccttctta gagggacaat atggtaattc     2760

cgacgtatat gtcggagtac cggcaattat taataggcaa ggtatacgtg aagtcgtaga     2820

gataaaactt aatgagaagg agcaagaaca gtttaatcac agtgttaagg ttttaaagga     2880

gaccatggcc cccattttgt aagagattga taagactttt ctagttgcat atcttttata     2940

tttaaatctt atctattagt taattttttg taatttatcc ttatatatag tctggttatt     3000

ctaaaatatc atttcagtat ctaaaaattc ccctcttttt tcagttatat cttaacaggc     3060

gacagtccaa atgttgattt atcccagtcc gattcatcag ggttgtgaag cattttgtca     3120

atggtcgaaa tcacatcagt aatagtgcct cttacttgcc tcatagaatt tctttctctt     3180

aacgtcaccg tttggtcttt tatagtttcg aaatctatgg tgataccaaa tggtgttccc     3240

aattcatcgt tacgggcgta ttttttacca attgaagtat tggaatcgtc aattttaaag     3300

tatatctctc ttttacgtaa agcctgcgag atcctcttaa gtatagcggg gaagccatcg     3360

ttattcgata ttgtcgtaac aaatactttg atcggcgcta tctgtaatgg aaaccaatac     3420

tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact ttatttagtc     3480

aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta     3540

cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc atccactaaa     3600

tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa     3660

tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca actacagaga     3720

acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc aacctgcctg     3780

gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt ttcttacacc     3840

ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt     3900

tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg tattgattgt     3960

aattctgtaa atctatttct taaacttctt aaattctact tttatagtta gtcttttttt     4020

tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa caaaatgtcg     4080

actaagagtt actcggaaag agcagctgct catagaagtc cagttgctgc caagctttta     4140

aacttgatgg aagagaagaa gtcaaactta tgtgcttctc ttgatgttcg taaaacagca     4200

gagttgttaa aattagtcga ggttttgggt ccatatatct gtctattgaa gacacatgta     4260

gatatcttgg aggatttcag ctttgagaat accattgtgc cgttgaagca attagcagag     4320

aaacacaagt ttttgatatt tgaagacagg aagtttgccg acattgggaa cactgttaaa     4380

ttacaataca cgtctggtgt ataccgtatc gccgaatggt ctgatatcac caatgcacac     4440

ggtgtgactg gtgcgggcat tgttgctggt ttgaagcaag gtgccgagga agttacgaaa     4500

gaacctagag ggttgttaat gcttgccgag ttatcgtcca aggggtctct agcgcacggt     4560

gaatacactc gtgggaccgt ggaaattgct aagagtgata aggactttgt tattggattt     4620

attgctcaaa acgatatggg tggaagagaa gagggctacg attggttgat catgacgcca     4680

ggtgttggtc ttgatgacaa aggtgatgct ttgggacaac aatacagaac tgtggatgaa     4740

gttgttgccg gtggatcaga catcattatt gttggtagag gtcttttcgc aaagggaaga     4800

gatcctgtag tggaaggtga gagatacaga aaggcgggat gggacgctta cttgaagaga     4860

gtaggcagat ccgcttaatc atgtaattag ttatgtcacg cttacattca cgccctcccc     4920

ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt     4980

atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt     5040

ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt     5100

tgggacgctc gaaggcttta atttgcgtga tgctaacttc tctctggaag gtctgaccgg     5160

ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat     5220

gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc     5280

agcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga     5340

cgttatctct ctgcactgcc cgctgacacc ggaaaactat catctgttga acgaagccgc     5400

cttcgaacag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga     5460

ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt     5520

gtatgagaac gaacgcgatc tattctttga agataaatcc aacgacgtga tccaggatga     5580

cgtattccgt cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct     5640

gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga     5700

aaaaggcgaa acctgcccga acgaactggt tggacataag cctgttcggt tcgtaagctg     5760

taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt     5820

ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct     5880

atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg     5940

agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg     6000

gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc     6060

catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg     6120

aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg     6180

cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt     6240

ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca     6300

gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc     6360

gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc     6420

gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta     6480

tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat     6540

gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc     6600

gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc     6660

gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc     6720

gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc     6780

aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc     6840

gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta     6900

ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg     6960

gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag     7020

cgagggcttt actaagctga tccggtggat gaccttttga atgaccttta atagattata     7080

ttactaatta attggggacc ctagaggtcc ccttttttat tttaaaaatt ttttcacaaa     7140

acggtttaca agcatacgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga     7200

ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc     7260

ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca     7320

atttcacaca ggaaacagct atgaccatga ttacgccaag cttgcatgcc tgcaggtcga     7380

ctctagagga tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg     7440

actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca     7500

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga     7560

atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc     7620

gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac     7680

acccgccaac acccgctgac gaattcgttg acagtaagac gggtaagcct gttgatgata     7740

ccgctgcctt actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt     7800

ggtcagactg gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca     7860

catagcagac ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg     7920

ggtagtttcc ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact     7980

gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg     8040

atggtcggag acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa     8100

gcctttaggg ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt     8160

gtaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt     8220

tatctttttt tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa     8280

aacacacaaa ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc     8340

aaaggtctag cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca     8400

ttgactagcc catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgt       8458


