                         SEQUENCE LISTING

<110>  Fornia BioSolutions, Inc.
       Zhang, Xiyun
       Yang, Jie
       Banerjee, Goutami
       Oo, Khin
       Palatino, Marielle  De Jesus
       Hu, Eric  Lin
       Sawaya, Imad  N.
       Fukushima, Tatsuya
       Zeng, Jijiao
       Lu, Wenhua
 
<120>  VARIANT G6P G7P GLUCOAMYLASE COMPOSITIONS AND METHODS

<130>  114095-5008

<150>  US 62/784,193
<151>  2018-12-21

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  620
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G6P preprotein amino acid sequence

<400>  1

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly Ser Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu Val Thr Phe Asn Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn 
        515                 520                 525             


Ile Lys Leu Val Gly Ser Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser 
    530                 535                 540                 


Ala Gly Ile Leu Leu Ser Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu 
545                 550                 555                 560 


Trp Thr Thr Thr Val Ser Val Pro Gln Gly Ser Thr Val Glu Phe Lys 
                565                 570                 575     


Phe Ile Arg Val Gly Ser Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn 
            580                 585                 590         


Asn Lys Val Leu Thr Val Gly Ser Ser Ala Thr Ser Val Thr Val Ser 
        595                 600                 605             


Ala Ser Trp Asn Gly Ala Tyr Ser Val Ser Ser Ser 
    610                 615                 620 


<210>  2
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G6P preprotein-encoding nucleic acid sequence

<400>  2
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggttca tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtctact acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cagctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tggtgacttt caacgagctg     1560

gctactacct actacggcga gaacattaag cttgtcggca gcacagctgc tttcggttcg     1620

tggtcgccct cagctggtat tctcctgtct gcctcgtcgt acacggccag caaccctctg     1680

tggactacca ccgtgtcggt tccccagggc tcgaccgttg agttcaagtt catccgtgtt     1740

ggctccgacg gcagcatcac gtgggagagc ggcaacaaca aggtgttgac ggttggctct     1800

tcggccacga gcgtcactgt ttctgccagc tggaacggcg cctactcggt gtctagctct     1860

taatag                                                                1866


<210>  3
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G7P preprotein amino acid sequence

<400>  3

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly Ser Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu 
        


<210>  4
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G7P preprotein-encoding nucleic acid sequence

<400>  4
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggttca tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtctact acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cagctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tgtaatag                  1548


<210>  5
<211>  620
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F with Starch Binding Domain) preprotein amino acid
       sequence

<400>  5

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly Ser Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Phe Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu Val Thr Phe Asn Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn 
        515                 520                 525             


Ile Lys Leu Val Gly Ser Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser 
    530                 535                 540                 


Ala Gly Ile Leu Leu Ser Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu 
545                 550                 555                 560 


Trp Thr Thr Thr Val Ser Val Pro Gln Gly Ser Thr Val Glu Phe Lys 
                565                 570                 575     


Phe Ile Arg Val Gly Ser Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn 
            580                 585                 590         


Asn Lys Val Leu Thr Val Gly Ser Ser Ala Thr Ser Val Thr Val Ser 
        595                 600                 605             


Ala Ser Trp Asn Gly Ala Tyr Ser Val Ser Ser Ser 
    610                 615                 620 


<210>  6
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F with Starch Binding Domain) preprotein-encoding 
       nucleic acid sequence

<400>  6
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggttca tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtcttct acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cagctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tggtgacttt caacgagctg     1560

gctactacct actacggcga gaacattaag cttgtcggca gcacagctgc tttcggttcg     1620

tggtcgccct cagctggtat tctcctgtct gcctcgtcgt acacggccag caaccctctg     1680

tggactacca ccgtgtcggt tccccagggc tcgaccgttg agttcaagtt catccgtgtt     1740

ggctccgacg gcagcatcac gtgggagagc ggcaacaaca aggtgttgac ggttggctct     1800

tcggccacga gcgtcactgt ttctgccagc tggaacggcg cctactcggt gtctagctct     1860

taatag                                                                1866


<210>  7
<211>  620
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P with Starch Binding Domain) 
       preprotein amino acid sequence

<400>  7

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly His Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Trp Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Pro Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu Val Thr Phe Asn Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn 
        515                 520                 525             


Ile Lys Leu Val Gly Ser Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser 
    530                 535                 540                 


Ala Gly Ile Leu Leu Ser Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu 
545                 550                 555                 560 


Trp Thr Thr Thr Val Ser Val Pro Gln Gly Ser Thr Val Glu Phe Lys 
                565                 570                 575     


Phe Ile Arg Val Gly Ser Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn 
            580                 585                 590         


Asn Lys Val Leu Thr Val Gly Ser Ser Ala Thr Ser Val Thr Val Ser 
        595                 600                 605             


Ala Ser Trp Asn Gly Ala Tyr Ser Val Ser Ser Ser 
    610                 615                 620 


<210>  8
<211>  1866
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P with Starch Binding Domain) 
       preprotein-encoding nucleic acid sequence

<400>  8
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggtcac tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtctggt acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cccctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tggtgacttt caacgagctg     1560

gctactacct actacggcga gaacattaag cttgtcggca gcacagctgc tttcggttcg     1620

tggtcgccct cagctggtat tctcctgtct gcctcgtcgt acacggccag caaccctctg     1680

tggactacca ccgtgtcggt tccccagggc tcgaccgttg agttcaagtt catccgtgtt     1740

ggctccgacg gcagcatcac gtgggagagc ggcaacaaca aggtgttgac ggttggctct     1800

tcggccacga gcgtcactgt ttctgccagc tggaacggcg cctactcggt gtctagctct     1860

taatag                                                                1866


<210>  9
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F without Starch Binding Domain) preprotein amino 
       acid sequence

<400>  9

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly Ser Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Phe Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu 
        


<210>  10
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F without Starch Binding Domain) 
       preprotein-encoding nucleic acid sequence

<400>  10
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggttca tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtcttct acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cagctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tgtaatag                  1548


<210>  11
<211>  514
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P without Starch Binding Domain) 
       preprotein amino acid sequence

<400>  11

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala Ser Pro Val Ser Lys Arg Ala Thr Leu Asp 
            20                  25                  30          


Glu Phe Ile Ser Thr Glu Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys 
        35                  40                  45              


Asn Ile Gly Pro Thr Gly Cys Arg Ala Ser Gly Ala Ala Ser Gly Val 
    50                  55                  60                  


Val Ile Ala Ser Pro Ser Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp 
65                  70                  75                  80  


Thr Arg Asp Ala Ala Leu Val Phe Lys Glu Ile Val Asp Ser Val Glu 
                85                  90                  95      


Thr Asn Thr Thr Leu Leu Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala 
            100                 105                 110         


Gln Ala Tyr Leu Gln Thr Val Thr Asn Pro Ser Gly Ser Leu Ser Asp 
        115                 120                 125             


Gly Ala Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Met Thr Pro Phe 
    130                 135                 140                 


Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 


Thr Ala Met Ile Ala Tyr Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr 
                165                 170                 175     


Thr Asp Cys Gly Leu Trp Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val 
            180                 185                 190         


Ala Gln Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro 
        195                 200                 205             


Gly His Ser Phe Phe Thr Val Ala Ala Gln Tyr Arg Ala Leu Val Glu 
    210                 215                 220                 


Gly Ser Thr Leu Ala Ala Lys Leu Gly Lys Ser His Ser Ala Tyr Asp 
225                 230                 235                 240 


Thr Val Ala Pro Gln Ile Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser 
                245                 250                 255     


Ser Lys Gly Tyr Ile Val Ala Asn Thr Gln Thr Ala Ser Trp Val Ser 
            260                 265                 270         


Arg Ser Gly Leu Asp Ala Asn Thr Pro Leu Thr Ala Ile His Leu Phe 
        275                 280                 285             


Asp Pro Glu Leu Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro 
    290                 295                 300                 


Lys Gln Leu Ile Thr Thr Lys Lys Leu Val Asp Ser Phe Arg Ser Ile 
305                 310                 315                 320 


Tyr Ala Ile Asn Ser Gly Lys Ser Ala Gly Asp Ala Leu Ala Val Gly 
                325                 330                 335     


Arg Tyr Ala Glu Asp Val Trp Tyr Asn Gly Asn Pro Trp Tyr Leu Cys 
            340                 345                 350         


Thr Leu Ala Val Ala Glu Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys 
        355                 360                 365             


Leu Glu Gly Ser Ile Thr Val Thr Ser Val Ser Leu Pro Phe Phe Thr 
    370                 375                 380                 


Asp Leu Leu Pro Ser Leu Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr 
385                 390                 395                 400 


Thr Phe Glu Ser Ile Ile Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe 
                405                 410                 415     


Val Ser Ile Val Gln Thr Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu 
            420                 425                 430         


Gln Tyr Ser Lys Tyr Asn Gly Gln Pro Leu Ser Ala Pro Asp Leu Thr 
        435                 440                 445             


Trp Ser Tyr Ala Ala Phe Leu Ser Ala Thr Glu Arg Arg Asp Ser Val 
    450                 455                 460                 


Val Pro Ala Gly Trp Ala Gly Ala Ser Ser Val Ser Val Pro Gly Ala 
465                 470                 475                 480 


Cys Ala Ala Thr Thr Val Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys 
                485                 490                 495     


Gly Thr Pro Gly Ser Gly Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn 
            500                 505                 510         


Ala Leu 
        


<210>  12
<211>  1548
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P without Starch Binding Domain) 
       preprotein-encoding nucleic acid sequence

<400>  12
atggttttcc tcaagtcggc catcgccgct tccacctggc tcttggctgc cactggcgtc       60

gttgcctcgc ctgtttccaa gcgcgctacg ctggacgagt tcatcagcac cgagcgtccc      120

ttggctctgg agcgcctgct ctgcaacatt ggtcctactg gttgccgtgc ttcgggagct      180

gcctcgggag tcgttatcgc ctcgccgtcc cgcagcgatc cggactacta ctacacttgg      240

acccgtgatg ctgctctggt ctttaaggag attgtcgact ctgtcgagac taacaccact      300

ctgctgctgc cagagattga gaactacgtt actgcccagg cttacctgca gaccgtgacg      360

aacccctcgg gttcgctgtc ggatggtgct ggtctgggcg agcccaagtt caacgtcgat      420

atgactccct tcactggtgc ctggggtcgt cctcagcgtg atggtccggc tctgcgtgct      480

acggctatga tcgcctacta caactacctg ctcaacaaca acgccactac cgactgtggt      540

ctgtggcaga ttatccagaa cgacctgaat tacgtcgctc agtactggaa ccaaactggt      600

tacgacctgt gggaggaggt tccgggtcac tcctttttca ctgttgctgc tcagtacaga      660

gctctcgttg agggttctac ccttgctgcc aagctcggca agtctcactc ggcctacgac      720

actgtcgctc cgcagattct gtgctacttg cagagcttct ggtcatccag caagggctac      780

attgtcgcca acacccagac tgccagctgg gtctcgcggt ccggtcttga tgccaacact      840

cccttgactg ccatccacct atttgaccct gaacttggct gcgatgactc gactttccag      900

ccctgctcgc ccaagcagct tatcactact aagaagctcg ttgactcgtt ccgctccatc      960

tatgccatca actcgggcaa gtctgctggt gatgctttgg ctgttggtcg ttacgccgag     1020

gacgtctggt acaacggcaa cccctggtac ctgtgcactt tggctgttgc agagcagctt     1080

tacgatgcag tttacacttg gaagctcgag ggctccatca ccgtcacctc tgtctcgctg     1140

cccttcttca ctgacctgct gccctcgctg accactggca cctacgcttc gggctcgacc     1200

accttcgaat ccatcatctc tgctgtgact acctacgctg atggctttgt cagtattgtc     1260

cagacctaca ctccctctga cggcgctctg tctgagcagt actccaagta caacggccag     1320

cccctgtcgg ctcccgacct gacctggtcg tacgccgctt tcctatctgc cactgagcgc     1380

cgtgacagcg ttgtccctgc cggctgggct ggtgcctcgt ctgtctctgt gcccggcgcc     1440

tgcgctgcta ccaccgttgt cggaacctac gctgctgcct ccaactgcgg tactcctggc     1500

tctggctcgg gcggcaacgg tggctcgagc ggtaacgccc tgtaatag                  1548


<210>  13
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G6P mature protein amino acid sequence

<400>  13

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu Val Thr Phe Asn 
                485                 490                 495     


Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn Ile Lys Leu Val Gly Ser 
            500                 505                 510         


Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser Ala Gly Ile Leu Leu Ser 
        515                 520                 525             


Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu Trp Thr Thr Thr Val Ser 
    530                 535                 540                 


Val Pro Gln Gly Ser Thr Val Glu Phe Lys Phe Ile Arg Val Gly Ser 
545                 550                 555                 560 


Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn Asn Lys Val Leu Thr Val 
                565                 570                 575     


Gly Ser Ser Ala Thr Ser Val Thr Val Ser Ala Ser Trp Asn Gly Ala 
            580                 585                 590         


Tyr Ser Val Ser Ser Ser 
        595             


<210>  14
<211>  1800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G6P mature protein-encoding nucleic acid sequence

<400>  14
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg ttcatccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

tactacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcagctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctggtga ctttcaacga gctggctact     1500

acctactacg gcgagaacat taagcttgtc ggcagcacag ctgctttcgg ttcgtggtcg     1560

ccctcagctg gtattctcct gtctgcctcg tcgtacacgg ccagcaaccc tctgtggact     1620

accaccgtgt cggttcccca gggctcgacc gttgagttca agttcatccg tgttggctcc     1680

gacggcagca tcacgtggga gagcggcaac aacaaggtgt tgacggttgg ctcttcggcc     1740

acgagcgtca ctgtttctgc cagctggaac ggcgcctact cggtgtctag ctcttaatag     1800


<210>  15
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  G7P mature protein amino acid sequence

<400>  15

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu 
                485                 490         


<210>  16
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  G7P mature protein-encoding nucleic acid sequence

<400>  16
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg ttcatccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

tactacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcagctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctgtaat ag                        1482


<210>  17
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F with Starch Binding Domain) mature protein amino 
       acid sequence

<400>  17

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Phe Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu Val Thr Phe Asn 
                485                 490                 495     


Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn Ile Lys Leu Val Gly Ser 
            500                 505                 510         


Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser Ala Gly Ile Leu Leu Ser 
        515                 520                 525             


Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu Trp Thr Thr Thr Val Ser 
    530                 535                 540                 


Val Pro Gln Gly Ser Thr Val Glu Phe Lys Phe Ile Arg Val Gly Ser 
545                 550                 555                 560 


Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn Asn Lys Val Leu Thr Val 
                565                 570                 575     


Gly Ser Ser Ala Thr Ser Val Thr Val Ser Ala Ser Trp Asn Gly Ala 
            580                 585                 590         


Tyr Ser Val Ser Ser Ser 
        595             


<210>  18
<211>  1800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F with Starch Binding Domain) mature 
       protein-encoding nucleic acid sequence

<400>  18
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg ttcatccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

ttctacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcagctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctggtga ctttcaacga gctggctact     1500

acctactacg gcgagaacat taagcttgtc ggcagcacag ctgctttcgg ttcgtggtcg     1560

ccctcagctg gtattctcct gtctgcctcg tcgtacacgg ccagcaaccc tctgtggact     1620

accaccgtgt cggttcccca gggctcgacc gttgagttca agttcatccg tgttggctcc     1680

gacggcagca tcacgtggga gagcggcaac aacaaggtgt tgacggttgg ctcttcggcc     1740

acgagcgtca ctgtttctgc cagctggaac ggcgcctact cggtgtctag ctcttaatag     1800


<210>  19
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P with Starch Binding Domain) mature 
       protein amino acid sequence

<400>  19

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly His Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Trp Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Pro Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu Val Thr Phe Asn 
                485                 490                 495     


Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn Ile Lys Leu Val Gly Ser 
            500                 505                 510         


Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser Ala Gly Ile Leu Leu Ser 
        515                 520                 525             


Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu Trp Thr Thr Thr Val Ser 
    530                 535                 540                 


Val Pro Gln Gly Ser Thr Val Glu Phe Lys Phe Ile Arg Val Gly Ser 
545                 550                 555                 560 


Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn Asn Lys Val Leu Thr Val 
                565                 570                 575     


Gly Ser Ser Ala Thr Ser Val Thr Val Ser Ala Ser Trp Asn Gly Ala 
            580                 585                 590         


Tyr Ser Val Ser Ser Ser 
        595             


<210>  20
<211>  1800
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P with Starch Binding Domain) mature 
       protein-encoding nucleic acid sequence

<400>  20
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg tcactccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

tggtacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcccctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctggtga ctttcaacga gctggctact     1500

acctactacg gcgagaacat taagcttgtc ggcagcacag ctgctttcgg ttcgtggtcg     1560

ccctcagctg gtattctcct gtctgcctcg tcgtacacgg ccagcaaccc tctgtggact     1620

accaccgtgt cggttcccca gggctcgacc gttgagttca agttcatccg tgttggctcc     1680

gacggcagca tcacgtggga gagcggcaac aacaaggtgt tgacggttgg ctcttcggcc     1740

acgagcgtca ctgtttctgc cagctggaac ggcgcctact cggtgtctag ctcttaatag     1800


<210>  21
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F without Starch Binding Domain) mature protein 
       amino acid sequence

<400>  21

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Phe Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Gln Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu 
                485                 490         


<210>  22
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant1 (Y321F without Starch Binding Domain) mature 
       protein-encoding nucleic acid sequence

<400>  22
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg ttcatccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

ttctacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcagctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctgtaat ag                        1482


<210>  23
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P without Starch Binding Domain) mature
       protein amino acid sequence

<400>  23

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Arg Leu Leu Cys Asn Ile Gly Pro Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ala Ser Gly Val Val Ile Ala Ser Pro Ser 
        35                  40                  45              


Arg Ser Asp Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Gly Glu 
            100                 105                 110         


Pro Lys Phe Asn Val Asp Met Thr Pro Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly His Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Asp Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Trp Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Ser Lys Tyr Asn 
                405                 410                 415     


Gly Gln Pro Leu Ser Ala Pro Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu 
                485                 490         


<210>  24
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant2 (S188H/Y321W/Q419P without Starch Binding Domain) mature
       protein-encoding nucleic acid sequence

<400>  24
tcgcctgttt ccaagcgcgc tacgctggac gagttcatca gcaccgagcg tcccttggct       60

ctggagcgcc tgctctgcaa cattggtcct actggttgcc gtgcttcggg agctgcctcg      120

ggagtcgtta tcgcctcgcc gtcccgcagc gatccggact actactacac ttggacccgt      180

gatgctgctc tggtctttaa ggagattgtc gactctgtcg agactaacac cactctgctg      240

ctgccagaga ttgagaacta cgttactgcc caggcttacc tgcagaccgt gacgaacccc      300

tcgggttcgc tgtcggatgg tgctggtctg ggcgagccca agttcaacgt cgatatgact      360

cccttcactg gtgcctgggg tcgtcctcag cgtgatggtc cggctctgcg tgctacggct      420

atgatcgcct actacaacta cctgctcaac aacaacgcca ctaccgactg tggtctgtgg      480

cagattatcc agaacgacct gaattacgtc gctcagtact ggaaccaaac tggttacgac      540

ctgtgggagg aggttccggg tcactccttt ttcactgttg ctgctcagta cagagctctc      600

gttgagggtt ctacccttgc tgccaagctc ggcaagtctc actcggccta cgacactgtc      660

gctccgcaga ttctgtgcta cttgcagagc ttctggtcat ccagcaaggg ctacattgtc      720

gccaacaccc agactgccag ctgggtctcg cggtccggtc ttgatgccaa cactcccttg      780

actgccatcc acctatttga ccctgaactt ggctgcgatg actcgacttt ccagccctgc      840

tcgcccaagc agcttatcac tactaagaag ctcgttgact cgttccgctc catctatgcc      900

atcaactcgg gcaagtctgc tggtgatgct ttggctgttg gtcgttacgc cgaggacgtc      960

tggtacaacg gcaacccctg gtacctgtgc actttggctg ttgcagagca gctttacgat     1020

gcagtttaca cttggaagct cgagggctcc atcaccgtca cctctgtctc gctgcccttc     1080

ttcactgacc tgctgccctc gctgaccact ggcacctacg cttcgggctc gaccaccttc     1140

gaatccatca tctctgctgt gactacctac gctgatggct ttgtcagtat tgtccagacc     1200

tacactccct ctgacggcgc tctgtctgag cagtactcca agtacaacgg ccagcccctg     1260

tcggctcccg acctgacctg gtcgtacgcc gctttcctat ctgccactga gcgccgtgac     1320

agcgttgtcc ctgccggctg ggctggtgcc tcgtctgtct ctgtgcccgg cgcctgcgct     1380

gctaccaccg ttgtcggaac ctacgctgct gcctccaact gcggtactcc tggctctggc     1440

tcgggcggca acggtggctc gagcggtaac gccctgtaat ag                        1482


<210>  25
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of wild type glucoamylase mature protein with
       Starch Binding Domain

<400>  25

Ser Pro Val Ser Lys Arg Ala Thr Leu Asp Glu Phe Ile Ser Thr Glu 
1               5                   10                  15      


Arg Pro Leu Ala Leu Glu Lys Leu Leu Cys Asn Ile Gly Ser Thr Gly 
            20                  25                  30          


Cys Arg Ala Ser Gly Ala Ser Ser Gly Val Val Leu Ala Ser Pro Ser 
        35                  40                  45              


Thr Ser Asn Pro Asp Tyr Tyr Tyr Thr Trp Thr Arg Asp Ala Ala Leu 
    50                  55                  60                  


Val Phe Lys Glu Ile Val Asp Ser Val Glu Thr Asn Thr Thr Leu Leu 
65                  70                  75                  80  


Leu Pro Glu Ile Glu Asn Tyr Val Thr Ala Gln Ala Tyr Leu Gln Thr 
                85                  90                  95      


Val Thr Asn Pro Ser Gly Ser Leu Ser Asp Gly Ala Gly Leu Ala Glu 
            100                 105                 110         


Pro Lys Phe Asn Ala Asp Leu Thr Gln Phe Thr Gly Ala Trp Gly Arg 
        115                 120                 125             


Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Ala Tyr 
    130                 135                 140                 


Tyr Asn Tyr Leu Leu Asn Asn Asn Ala Thr Thr Asp Cys Gly Leu Trp 
145                 150                 155                 160 


Gln Ile Ile Gln Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln 
                165                 170                 175     


Thr Gly Tyr Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr 
            180                 185                 190         


Val Ala Ala Gln Tyr Arg Ala Leu Val Glu Gly Ser Thr Leu Ala Ala 
        195                 200                 205             


Lys Leu Gly Lys Ser His Ser Ala Tyr Asp Thr Val Ala Pro Gln Ile 
    210                 215                 220                 


Leu Cys Tyr Leu Gln Ser Phe Trp Ser Ser Ser Lys Gly Tyr Ile Val 
225                 230                 235                 240 


Ala Asn Thr Gln Thr Ala Ser Trp Val Ser Arg Ser Gly Leu Asp Ala 
                245                 250                 255     


Asn Thr Pro Leu Thr Ala Ile His Leu Phe Asp Pro Glu Leu Gly Cys 
            260                 265                 270         


Asp Asp Ser Thr Phe Gln Pro Cys Ser Pro Lys Gln Leu Ile Thr Thr 
        275                 280                 285             


Lys Lys Leu Val Asp Ser Phe Arg Ser Ile Tyr Ala Ile Asn Ser Gly 
    290                 295                 300                 


Lys Ser Ala Gly Thr Ala Leu Ala Val Gly Arg Tyr Ala Glu Asp Val 
305                 310                 315                 320 


Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Val Ala Glu 
                325                 330                 335     


Gln Leu Tyr Asp Ala Val Tyr Thr Trp Lys Leu Glu Gly Ser Ile Thr 
            340                 345                 350         


Val Thr Ser Val Ser Leu Pro Phe Phe Thr Asp Leu Leu Pro Ser Leu 
        355                 360                 365             


Thr Thr Gly Thr Tyr Ala Ser Gly Ser Thr Thr Phe Glu Ser Ile Ile 
    370                 375                 380                 


Ser Ala Val Thr Thr Tyr Ala Asp Gly Phe Val Ser Ile Val Gln Thr 
385                 390                 395                 400 


Tyr Thr Pro Ser Asp Gly Ala Leu Ser Glu Gln Tyr Asn Lys Ala Asn 
                405                 410                 415     


Gly Gln Gln Leu Ser Ala Gln Asp Leu Thr Trp Ser Tyr Ala Ala Phe 
            420                 425                 430         


Leu Ser Ala Thr Glu Arg Arg Asp Ser Val Val Pro Ala Gly Trp Ala 
        435                 440                 445             


Gly Ala Ser Ser Val Ser Val Pro Gly Ala Cys Ala Ala Thr Thr Val 
    450                 455                 460                 


Val Gly Thr Tyr Ala Ala Ala Ser Asn Cys Gly Thr Pro Gly Ser Gly 
465                 470                 475                 480 


Ser Gly Gly Asn Gly Gly Ser Ser Gly Asn Ala Leu Val Thr Phe Asn 
                485                 490                 495     


Glu Leu Ala Thr Thr Tyr Tyr Gly Glu Asn Ile Lys Leu Val Gly Ser 
            500                 505                 510         


Thr Ala Ala Phe Gly Ser Trp Ser Pro Ser Ala Gly Ile Leu Leu Ser 
        515                 520                 525             


Ala Ser Ser Tyr Thr Ala Ser Asn Pro Leu Trp Thr Thr Thr Val Ser 
    530                 535                 540                 


Val Pro Gln Gly Ser Thr Val Glu Phe Lys Phe Ile Arg Val Gly Ser 
545                 550                 555                 560 


Asp Gly Ser Ile Thr Trp Glu Ser Gly Asn Asn Lys Val Leu Thr Val 
                565                 570                 575     


Gly Ser Ser Ala Thr Ser Val Thr Val Ser Ala Ser Trp Asn Gly Ala 
            580                 585                 590         


Tyr Ser Val Ser Ser Ser 
        595             


<210>  26
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of wild type signal peptide

<400>  26

Met Val Phe Leu Lys Ser Ala Ile Ala Ala Ser Thr Trp Leu Leu Ala 
1               5                   10                  15      


Ala Thr Gly Val Val Ala 
            20          


