			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> HUMAN ALPHA-GALACTOSIDASE VARIANTS
 
<130> CX7-184WO2
 
<140> PCT/US2019/067493
<141> 2019-12-19
 
<150> US 62/782,553
<151> 2018-12-20
 
<160> 702
 
<210> 1
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> wild type human alpha-galactosidase A
 
<400> 1
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac taatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggagcat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttcttt caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa tcaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggacttgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaagttgg gtttctatga gtggacatct aggctaagaa gtcacatcaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta       1194

 
<210> 2
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> wild type human alpha-galactosidase A
 
<400> 2
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 3
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 3
ttggataacg ggttagcccg tacacctact atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcatggag 120
atggctgaac ggatggtaag tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactacgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
gcacccctat tcatgtctaa tgatctacgt cacatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga atggacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaatgtctt tgaaagattt gtta       1194

 
<210> 4
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 4
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 5
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 5
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctataa cgcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 6
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 6
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Asn Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 7
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 7
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 8
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 8
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 9
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 9
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 10
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 10
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 11
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 11
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 12
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 12
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 13
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 13
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 14
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 14
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 15
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 15
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 16
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 16
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 17
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 17
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 18
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 18
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 19
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 19
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 20
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 20
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 21
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 21
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 22
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 22
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 23
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 23
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 24
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 24
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 25
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 25
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agacaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 26
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 26
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 27
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 27
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 28
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 28
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 29
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 29
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 30
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 30
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 31
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 31
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 32
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 32
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 33
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 33
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 34
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 34
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 35
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 35
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 36
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 36
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 37
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 37
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 38
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 38
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 39
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 39
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 40
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 40
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 41
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 41
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 42
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 42
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 43
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 43
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 44
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 44
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 45
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 45
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 46
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 46
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 47
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 47
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 48
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 48
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 49
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 49
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 50
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 50
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 51
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 51
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 52
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 52
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 53
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 53
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggaa ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 54
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 54
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 55
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 55
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggattagc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 56
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 56
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 57
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 57
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 58
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 58
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 59
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 59
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac tgatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttcttt taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctatgatca accgtcaaga gatcggaggg ccaagatctt acactatcgc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agacaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 60
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 60
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Gln Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 61
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 61
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtagggcgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagatgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaaaaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtta       1194

 
<210> 62
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 62
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ala Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Lys Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     


<210> 63
<211> 100
<212> DNA
<213> Artificial Sequence
 
<220>
<223> BamHI-pCDNA-GLA-F
 
<400> 63
acgtttggat cccgccacca tggaatggag ctgggtcttt ctcttcttcc tgtcagtaac 60
gactggtgtc cactccttgg ataacgggtt agcccgtaca                       100


<210> 64
<211> 35
<212> DNA
<213> Artificial Sequence
  
<220>
<223> XhoI-pCDNA-GLA-R
 
<400> 64
attgtactcg agttataaca aatctttcaa agaca 35


<210> 65
<211> 57
<212> DNA
<213> Artificial Sequence
 
<220>
<223> synthetic mouse IG signal peptide
 
<400> 65
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactcc 57


<210> 66
<211> 19
<212> PRT
<213> Artificial Sequence
 
<220>
<223> synthetic mouse IG signal peptide
 
<400> 66
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15   
Val His Ser 

<210> 67
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 67
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 68
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 68
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 69
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 69
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 70
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 70
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 71
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 71
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 72
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 72
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 73
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 73
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 74
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 74
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 75
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 75
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tgcccttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 76
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 76
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Ala Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 77
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 77
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 78
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 78
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 79
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 79
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 80
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 80
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 81
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 81
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 82
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 82
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 83
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 83
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 84
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 84
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 85
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 85
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 86
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 86
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 87
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 87
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 88
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 88
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 89
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 89
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggtcac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtctg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 90
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 90
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly His Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 91
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 91
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 92
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 92
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 93
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 93
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 94
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 94
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 95
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 95
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 96
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 96
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 97
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 97
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctac acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 98
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 98
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Thr Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 99
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 99
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 100
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 100
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 101
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 101
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 102
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 102
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 103
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 103
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaaatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 104
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 104
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 105
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 105
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 106
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 106
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 107
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 107
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 108
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 108
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 109
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 109
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc atttggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 110
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 110
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 111
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 111
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 112
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 112
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 113
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 113
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 114
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 114
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 115
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 115
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 116
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 116
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 117
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 117
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgggt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 118
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 118
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Gly Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 119
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 119
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 120
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 120
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 121
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 121
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcgtatc 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 122
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 122
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Arg Ile Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 123
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 123
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attcgctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 124
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 124
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 125
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 125
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 126
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 126
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 127
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 127
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acgggtttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 128
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 128
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Gly Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 129
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 129
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 130
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 130
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 131
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 131
ttggataacg ggttagccct gacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgtctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 132
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 132
Leu Asp Asn Gly Leu Ala Leu Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Val Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 133
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 133
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgatggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaactg 900
agaaaaggtg acaattttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 134
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 134
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 135
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 135
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 136
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 136
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 137
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 137
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 138
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 138
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 139
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 139
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 140
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 140
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 141
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 141
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 142
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 142
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 143
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 143
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agacaattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 144
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 144
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Gln Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 145
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 145
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aatcatgtac acagtctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 146
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 146
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 147
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 147
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataggcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 148
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 148
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Gly Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 149
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 149
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 150
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 150
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 151
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 151
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 152
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 152
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 153
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 153
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 154
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 154
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 155
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 155
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgacg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 156
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 156
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 157
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 157
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 158
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 158
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 159
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 159
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 160
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 160
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 161
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 161
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacagtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 162
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 162
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Ser Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 163
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 163
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acagtattgg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aaaacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 164
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 164
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Ser Ile 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys Asn Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 165
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 165
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 166
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 166
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 167
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 167
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca cagcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcagctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 168
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 168
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Gln His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 169
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 169
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 170
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 170
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 171
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 171
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 172
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 172
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 173
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 173
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 174
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 174
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 175
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 175
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtggcg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 176
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 176
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 177
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 177
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcataaa gcagcttgca aatcatgtac acgagctggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aaccatactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 178
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 178
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Lys Gln Leu Ala Asn His Val His Glu Leu 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn His Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 179
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 179
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataccgc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 180
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 180
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Pro Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 181
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 181
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaga cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg ccagcttgca aatcatgtac acgagaaggg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aaccgtactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaaag gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 182
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 182
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Glu Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 183
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 183
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat ggggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 184
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 184
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Gly Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 185
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 185
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacaagatg caaacctctt tgaaagattt gttataa    1197

 
<210> 186
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 186
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Lys Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 187
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 187
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgacg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 188
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 188
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Thr Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 189
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 189
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtgcgtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 190
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 190
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Ala Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 191
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 191
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccggtt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 192
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 192
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Val Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 193
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 193
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tgcggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 194
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 194
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Ala Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 195
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 195
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgctg 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 196
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 196
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Leu Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 197
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 197
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaaccggt aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 198
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 198
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Gly Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 199
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 199
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gtcttaa    1197

 
<210> 200
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 200
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ser         
385                 390                 395                     

 
<210> 201
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 201
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagatgc tttataa    1197

 
<210> 202
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 202
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Ala Leu         
385                 390                 395                     

 
<210> 203
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 203
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggaagtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 204
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 204
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 205
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 205
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggaagat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 206
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 206
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Lys Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 207
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 207
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aggatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 208
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 208
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Arg Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 209
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 209
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaaggttt aactcga    1197

 
<210> 210
<211> 399
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 210
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Gly Leu Thr Arg     
385                 390                 395                     

 
<210> 211
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 211
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gagtccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 212
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 212
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Ser Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 213
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 213
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgg cgggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 214
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 214
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ala Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 215
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 215
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat ccggaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 216
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 216
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Pro Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 217
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 217
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctcta agaaagattt gttataa    1197

 
<210> 218
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 218
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Lys Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 219
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 219
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
gctaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 220
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 220
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Ala Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 221
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 221
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgcggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 222
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 222
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Ala Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 223
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 223
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatattc acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 224
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 224
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Ile His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 225
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 225
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtcagtatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 226
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 226
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Gln Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 227
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 227
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactgtgcc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 228
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 228
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Val 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 229
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 229
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aactttgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 230
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 230
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Phe Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 231
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 231
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggtgtaa    1197

 
<210> 232
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 232
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Val         
385                 390                 395                     

 
<210> 233
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 233
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gccgtaa    1197

 
<210> 234
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 234
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Pro         
385                 390                 395                     

 
<210> 235
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 235
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
atgaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 236
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 236
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Met Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 237
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 237
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 238
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 238
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 239
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 239
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagagggt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 240
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 240
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 241
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 241
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaacagg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 242
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 242
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Gln Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 243
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 243
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt ggaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 244
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 244
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 245
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 245
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aacacggtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 246
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 246
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Thr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 247
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 247
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgttt tgaaagattt gttataa    1197

 
<210> 248
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 248
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 249
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 249
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 250
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 250
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 251
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 251
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggt 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatttt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 252
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 252
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 253
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 253
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcctg gtaaacaagg ttatcaatta 900
agaaagggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gctacctctt tgaaagattt gttataa    1197

 
<210> 254
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 254
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Pro Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Ala Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 255
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 255
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 256
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 256
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 257
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 257
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 258
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 258
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 259
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 259
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 260
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 260
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 261
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 261
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac cgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tttggctcta 480
aacaggactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgatatcac cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcttaa    1197

 
<210> 262
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 262
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 263
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 263
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 264
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 264
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 265
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 265
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 266
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 266
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 267
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 267
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg gcgaccgtat tgaaagattt gttataa    1197

 
<210> 268
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 268
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Ala Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 269
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 269
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctctgg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaactgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 270
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 270
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 271
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 271
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 272
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 272
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 273
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 273
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 274
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 274
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 275
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 275
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 276
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 276
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 277
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 277
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa    1197

 
<210> 278
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 278
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 279
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 279
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgacggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccgtat tgaaagattt gttataa    1197

 
<210> 280
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 280
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Asp 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 281
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 281
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 282
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 282
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 283
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 283
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc agagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 284
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 284
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Glu Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 285
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 285
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 286
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 286
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 287
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 287
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagaccagct tgaaagattt gttataa    1197

 
<210> 288
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 288
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 289
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 289
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagattct acactatccc ggtagcctct 1020
ttgggtaagg gtcaagcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 290
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 290
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Phe Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 291
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 291
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaggtt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 292
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 292
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Gly Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 293
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 293
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 294
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 294
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 295
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 295
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 296
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 296
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 297
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 297
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 298
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 298
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 299
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 299
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa    1197

 
<210> 300
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 300
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Gly Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 301
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 301
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 302
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 302
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 303
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 303
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 304
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 304
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 305
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 305
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 306
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 306
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 307
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 307
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
cgtaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 308
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 308
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 309
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 309
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 310
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 310
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 311
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 311
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 312
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 312
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 313
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 313
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcgtc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 314
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 314
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 315
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 315
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtgatg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 316
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 316
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Asp Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 317
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 317
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtagt tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt gctgtaa    1197

 
<210> 318
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 318
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Val Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 319
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 319
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catggtcgtc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 320
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 320
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ser Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 321
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 321
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 322
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 322
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 323
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 323
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt ggcgtaa    1197

 
<210> 324
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 324
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 325
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 325
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 326
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 326
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 327
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 327
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 328
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 328
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 329
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 329
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccg tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccagct tgaaagattt ggcgtaa    1197

 
<210> 330
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 330
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Arg Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 331
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 331
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caaaacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 332
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 332
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asn Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 333
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 333
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 334
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 334
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 335
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 335
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtgtcgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 336
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 336
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 337
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 337
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagacaagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtggtg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 338
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 338
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Gly Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 339
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 339
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 340
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 340
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 341
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 341
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 342
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 342
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 343
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 343
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctaa gaaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 344
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 344
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Lys Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 345
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 345
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgag tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg caaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 346
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 346
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Glu Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 347
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 347
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 348
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 348
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 349
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 349
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc ctggggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc tgtagcctct 1020
ttgggtaagg gtcaggcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt ggcgtaa    1197

 
<210> 350
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 350
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Leu Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Gln Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Ala         
385                 390                 395                     

 
<210> 351
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 351
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgtggag 120
atggctgaac ggatggtaga tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 352
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 352
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Val Glu Met Ala Glu Arg Met Val Asp Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 353
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 353
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tcgtgctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaaccgtat tgaaagattt gctgtaa    1197

 
<210> 354
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 354
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Arg Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Val Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 355
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 355
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctagcgatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 356
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 356
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Ala Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 357
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 357
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 358
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 358
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 359
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 359
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 360
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 360
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 361
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 361
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttat cgtgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
ataaatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagatctt acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 362
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 362
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Ser Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asn Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 363
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 363
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctactgcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 364
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 364
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 365
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 365
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgcatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaaact acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 366
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 366
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Asn Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 367
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 367
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt aatataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgga gaacacaatg caaacctctt tgaaagattt gttataa    1197

 
<210> 368
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 368
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Asn Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 369
<211> 1197
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 369
ttggataacg ggttagcccg tacacctccg atgggttggc ttcactggga aagattcatg 60
tgtaacttag attgccaaga agagcctgac agctgtatct cagagaaact attcgaagag 120
atggctgaac ggatggtaac tgaaggatgg aaggatgctg gttatgaata cctatgtatt 180
gatgattgct ggatggctcc acagcgtgat tcagaaggta ggttacaagc tgacccccag 240
agattcccac atggcatacg tcagcttgca aaccatgtac acagcaaagg tctaaagtta 300
ggcatctacg ctgatgtcgg aaacaagaca tgtgctggtt tcccaggttc attcggttac 360
tatgacatag atgcgcagac gtttgctgat tggggtgttg atttgttgaa gtttgatgga 420
tgctacagcg attccctgga gaacctagcc gatgggtaca aacacatgag tctggctcta 480
aacaaaactg gtaggccgat cgtctatagt tgtgaatggc ccttgtacat gtggccgttt 540
cagaagccaa actacactga gataagacaa tactgtaacc attggcgtaa ctttgctgac 600
atagatgatt catgggcttc aatcaaatct atcttggatt ggacttctcg taaccaggaa 660
agaattgttg atgttgcagg tccaggtgga tggaatgacc ctgatatgct tgtcataggg 720
aactttgggc tatcatggga ccaacaagtt acacaaatgg ctttgtgggc gatcatggcc 780
ggccccctat tcatgtctaa tgatctacgt gcgataagcc cccaagcaaa ggctttactt 840
caagataagg atgtcatagc gatcaaccaa gatcctcttg gtaaacaagg ttatcaattg 900
agaaaaggtg acaactttga agtgtgggaa agaccattgt ctggagacgc gtgggctgtt 960
gctattatca accgtcaaga gatcggaggg ccaagaagct acactatccc ggtagcctct 1020
ttgggtaagg gtgttgcgtg caatcctgcc tgcttcatta cacaattgct tccagttaag 1080
agaaaattgg gtttctatga agcaacctct aggctaagaa gtcacattaa tcctactggt 1140
acggtattgt tgcaattgaa caacacaatg cagacctctt tgaaagattt gttataa    1197

 
<210> 370
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 370
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Ser Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Lys Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Asn Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 371
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 371
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 372
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 372
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 373
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 373
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagacgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 374
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 374
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 375
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 375
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 376
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 376
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 377
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 377
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 378
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 378
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 379
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 379
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 380
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 380
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 381
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 381
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 382
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 382
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 383
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 383
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 384
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 384
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 385
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 385
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 386
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 386
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 387
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 387
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 388
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 388
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 389
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 389
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 390
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 390
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 391
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 391
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 392
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 392
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 393
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 393
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 394
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 394
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 395
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 395
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 396
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 396
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 397
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 397
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 398
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 398
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 399
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 399
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 400
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 400
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 401
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 401
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctatgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 402
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 402
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 403
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 403
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagatagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 404
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 404
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Ile Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 405
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 405
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 406
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 406
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 407
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 407
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 408
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 408
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 409
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 409
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
atgactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 410
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 410
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Met Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 411
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 411
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 412
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 412
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 413
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 413
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 414
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 414
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 415
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 415
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctcttt caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 416
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 416
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 417
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 417
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 418
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 418
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 419
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 419
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 420
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 420
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 421
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 421
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 422
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 422
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 423
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 423
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 424
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 424
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 425
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 425
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 426
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 426
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 427
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 427
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 428
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 428
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 429
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 429
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 430
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 430
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 431
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 431
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 432
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 432
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     
 
<210> 433
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 433
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 434
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 434
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 435
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 435
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 436
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 436
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 437
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 437
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 438
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 438
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 439
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 439
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 440
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 440
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 441
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 441
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 442
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 442
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 443
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 443
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 444
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 444
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 445
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 445
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 446
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 446
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 447
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 447
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 448
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 448
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 449
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 449
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 450
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 450
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 451
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 451
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 452
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 452
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 453
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 453
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 454
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 454
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 455
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 455
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 456
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 456
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 457
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 457
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 458
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 458
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 459
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 459
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct gggccagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 460
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 460
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Ala Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 461
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 461
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctact ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 462
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 462
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 463
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 463
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 464
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 464
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 465
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 465
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 466
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 466
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 467
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 467
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 468
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 468
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 469
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 469
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctggt 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcctcgcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 470
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 470
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 471
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 471
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagcgga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 472
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 472
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 473
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 473
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 474
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 474
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 475
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 475
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc ggatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 476
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 476
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 477
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 477
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 478
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 478
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 479
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 479
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt cgaggagatg 180
gcagagctga tggtctctga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctttcaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 480
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 480
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 481
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 481
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcgaggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 482
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 482
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 483
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 483
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 484
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 484
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 485
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 485
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 486
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 486
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 487
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 487
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 488
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 488
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 489
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 489
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtcac tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 490
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 490
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 491
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 491
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catctaggaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcgatgcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag acctcattaa aagacttact t          1251

 
<210> 492
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 492
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 493
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 493
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctga tggtcactga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaatca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgagcc atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 494
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 494
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Thr Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg Ala Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 495
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 495
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctccaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat tatgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctggaacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgcagt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaagc tacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 496
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 496
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Ala Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 497
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 497
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 498
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 498
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 499
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 499
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
ggtcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 500
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 500
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 501
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 501
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 502
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 502
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 503
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 503
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctatgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagacctcat taaaagactt actt       1194

 
<210> 504
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 504
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 505
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 505
ctggacaatg gattggcaag gacgcctcca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctgggccag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 506
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 506
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 507
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 507
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aattatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga agctacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 508
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 508
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 509
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 509
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatctag gaaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 510
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 510
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 511
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 511
ctggacaatg gattggcaag gacgcctacc atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt taaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcttagc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatccc cgttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 512
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 512
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 513
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 513
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaagag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa tcagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcgatgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 514
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 514
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 515
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 515
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaagagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
cagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
atgataaacc ggcaggagat tggtggacct cgctcttata ccatcgccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 516
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 516
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Gln Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 517
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 517
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tgatggtctc tgaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggga ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga gccatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agacagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctattataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 518
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 518
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 519
<211> 1251
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 519
atggaatgga gctgggtctt tctcttcttc ctgtcagtaa cgactggtgt ccactccctg 60
gacaatggat tggcaaggac gcctacaatg ggctggctgc actgggagcg cttcatgtgc 120
aaccttgact gccaggaaga gccagattcc tgcatcagtg agaagctctt catggagatg 180
gcagagctca tggtctcaga aggctggaag gatgcaggtt atgagtacct ctgcattgat 240
gactgttgga tggctcccca aagagattca gaaggcagac ttcaggcaga ccctcagcgc 300
tttcctcatg ggattcgcca gctagctaat catgttcaca gcaaaggact gaagctaggg 360
atttatgcag atgttggaaa taaaacctgc gcaggcttcc ctgggagttt tggatactac 420
gacattgatg cccagacctt tgctgactgg ggagtagatc tgctaaaatt tgatggttgt 480
tactgtgaca gtttggaaaa tttggcagat ggttataagc acatgtcctt ggccctgaat 540
aggactggca gaagcattgt gtactcctgt gagtggcctc tttatatgtg gccctttcaa 600
aagcccaatt atacagaaat ccgacagtac tgcaatcact ggcgaaattt tgctgacatt 660
gatgattcct ggaaaagtat aaagagtatc ttggactgga catcttttaa ccaggagaga 720
attgttgatg ttgctggacc agggggttgg aatgacccag atatgttagt gattggcaac 780
tttggcctca gctgggacca gcaagtaact cagatggccc tctgggctat catggctgct 840
cctttattca tgtctaatga cctccgacac atcagccctc aagccaaagc tctccttcag 900
gataaggacg taattgccat caatcaggac cccttgggca agcaagggta ccagcttaga 960
aagggagaca actttgaagt gtgggaacga cctctctcag gcttagcctg ggctgtagct 1020
attataaacc ggcaggagat tggtggacct cgctcttata ccatccccgt tgcttccctg 1080
ggtaaaggag tggcctgtaa tcctgcctgc ttcatcacac agctcctccc tgtgaaaagg 1140
aagctagggt tctatgaatg gacttcaagg ttaagaagtc acataaatcc cacaggcact 1200
gttttgcttc agctagaaaa tacaatgcag atgtcattaa aagacttact t          1251

 
<210> 520
<211> 417
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 520
Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      
Val His Ser Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp 
            20                  25                  30          
Leu His Trp Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro 
        35                  40                  45              
Asp Ser Cys Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met 
    50                  55                  60                  
Val Ser Glu Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp 
65                  70                  75                  80  
Asp Cys Trp Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala 
                85                  90                  95      
Asp Pro Gln Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val 
            100                 105                 110         
His Ser Lys Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys 
        115                 120                 125             
Thr Cys Ala Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala 
    130                 135                 140                 
Gln Thr Phe Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys 
145                 150                 155                 160 
Tyr Cys Asp Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser 
                165                 170                 175     
Leu Ala Leu Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp 
            180                 185                 190         
Pro Leu Tyr Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg 
        195                 200                 205             
Gln Tyr Cys Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp 
    210                 215                 220                 
Lys Ser Ile Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg 
225                 230                 235                 240 
Ile Val Asp Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu 
                245                 250                 255     
Val Ile Gly Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met 
            260                 265                 270         
Ala Leu Trp Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu 
        275                 280                 285             
Arg His Ile Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val 
    290                 295                 300                 
Ile Ala Ile Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg 
305                 310                 315                 320 
Lys Gly Asp Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala 
                325                 330                 335     
Trp Ala Val Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser 
            340                 345                 350         
Tyr Thr Ile Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro 
        355                 360                 365             
Ala Cys Phe Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe 
    370                 375                 380                 
Tyr Glu Trp Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr 
385                 390                 395                 400 
Val Leu Leu Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu 
                405                 410                 415     
Leu                                                             
                                                                

 
<210> 521
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 521
ctggacaatg gattggcaag gacgcctaca atgggctggc tgcactggga gcgcttcatg 60
tgcaaccttg actgccagga agagccagat tcctgcatca gtgagaagct cttcatggag 120
atggcagagc tcatggtctc agaaggctgg aaggatgcag gttatgagta cctctgcatt 180
gatgactgtt ggatggctcc ccaaagagat tcagaaggca gacttcaggc agaccctcag 240
cgctttcctc atgggattcg ccagctagct aatcatgttc acagcaaagg actgaagcta 300
gggatttatg cagatgttgg aaataaaacc tgcgcaggct tccctgggag ttttggatac 360
tacgacattg atgcccagac ctttgctgac tggggagtag atctgctaaa atttgatggt 420
tgttactgtg acagtttgga aaatttggca gatggttata agcacatgtc cttggccctg 480
aataggactg gcagaagcat tgtgtactcc tgtgagtggc ctctttatat gtggcccttt 540
caaaagccca attatacaga aatccgacag tactgcaatc actggcgaaa ttttgctgac 600
attgatgatt cctggaaaag tataaagagt atcttggact ggacatcttt caaccaggag 660
agaattgttg atgttgctgg accagggggt tggaatgacc cagatatgtt agtgattggc 720
aactttggcc tcagctggaa ccagcaagta actcagatgg ccctctgggc tatcatggct 780
gctcctttat tcatgtctaa tgacctccga cacatcagcc ctcaagccaa agctctcctt 840
caggataagg acgtaattgc catcaatcag gaccccttgg gcaagcaagg gtaccagctt 900
agaaagggag acaactttga agtgtgggaa cgacctctct caggcctcgc ctgggctgta 960
gctatgataa accggcagga gattggtgga cctcgctctt ataccatcgc agttgcttcc 1020
ctgggtaaag gagtggcctg taatcctgcc tgcttcatca cacagctcct ccctgtgaaa 1080
aggaagctag ggttctatga atggacttca aggttaagaa gtcacataaa tcccacaggc 1140
actgttttgc ttcagctaga aaatacaatg cagatgtcat taaaagactt actt       1194

 
<210> 522
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 522
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 523
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 523
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 524
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 524
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 525
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 525
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 526
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 526
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 527
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 527
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 528
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 528
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 529
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 529
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 530
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 530
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 531
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 531
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 532
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 532
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 533
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 533
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat gggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
caaaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 534
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 534
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Gly Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 535
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 535
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 536
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 536
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 537
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 537
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 538
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 538
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 539
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 539
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 540
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 540
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 541
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 541
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 542
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 542
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 543
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 543
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 544
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 544
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 545
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 545
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 546
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 546
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 547
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 547
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 548
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 548
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 549
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 549
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 550
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 550
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 551
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 551
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 552
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 552
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 553
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 553
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 554
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 554
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 555
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 555
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 556
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 556
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 557
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 557
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 558
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 558
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 559
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 559
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 560
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 560
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 561
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 561
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 562
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 562
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 563
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 563
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 564
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 564
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 565
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 565
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 566
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 566
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 567
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 567
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 568
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 568
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 569
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 569
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 570
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 570
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 571
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 571
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcgccaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 572
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 572
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 573
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 573
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 574
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 574
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 575
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 575
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 576
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 576
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 577
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 577
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 578
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 578
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 579
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 579
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 580
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 580
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 581
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 581
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 582
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 582
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 583
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 583
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 584
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 584
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 585
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 585
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaaaatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 586
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 586
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asn Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 587
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 587
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 588
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 588
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 589
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 589
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 590
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 590
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 591
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 591
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagatgct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 592
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 592
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Met Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 593
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 593
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 594
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 594
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 595
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 595
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 596
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 596
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 597
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 597
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 598
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 598
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 599
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 599
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 600
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 600
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 601
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 601
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 602
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 602
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 603
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 603
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 604
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 604
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 605
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 605
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 606
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 606
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 607
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 607
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 608
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 608
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 609
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 609
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 610
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 610
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 611
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 611
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 612
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 612
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 613
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 613
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 614
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 614
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 615
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 615
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 616
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 616
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 617
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 617
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 618
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 618
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 619
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 619
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 620
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 620
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 621
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 621
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 622
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 622
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 623
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 623
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 624
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 624
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 625
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 625
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 626
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 626
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 627
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 627
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 628
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 628
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 629
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 629
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 630
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 630
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 631
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 631
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 632
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 632
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 633
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 633
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 634
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 634
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 635
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 635
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 636
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 636
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 637
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 637
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 638
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 638
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 639
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 639
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 640
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 640
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 641
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 641
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 642
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 642
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 643
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 643
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 644
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 644
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 645
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 645
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 646
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 646
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 647
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 647
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 648
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 648
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 649
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 649
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 650
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 650
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 651
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 651
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 652
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 652
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 653
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 653
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga atggaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 654
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 654
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Trp 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 655
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 655
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 656
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 656
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 657
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 657
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 658
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 658
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 659
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 659
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 660
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 660
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 661
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 661
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 662
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 662
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 663
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 663
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaaacaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 664
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 664
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 665
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 665
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 666
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 666
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 667
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 667
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 668
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 668
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 669
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 669
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catggaagtc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 670
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 670
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Lys Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 671
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 671
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 672
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 672
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 673
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 673
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac tgatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 674
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 674
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Leu Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 675
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 675
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 676
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 676
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 677
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 677
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 678
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 678
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 679
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 679
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 680
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 680
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 681
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 681
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 682
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 682
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 683
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 683
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 684
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 684
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 685
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 685
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagttt taatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 686
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 686
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Phe Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 687
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 687
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 688
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 688
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 689
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 689
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac tgatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggaa tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agacagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 690
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 690
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Leu Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asn Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Gln Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 691
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 691
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agcaaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagatgtcac tgaaggacct cctg       1194

 
<210> 692
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 692
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Met Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 693
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 693
ctggataatg gattggctag aacacctccc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgtc cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 694
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 694
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Ser Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 695
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 695
ctggataatg gattggctag aacacctcct atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggccctcttt tcatgagcaa cgatctgcgc catatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 696
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 696
Leu Asp Asn Gly Leu Ala Arg Thr Pro Pro Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg His Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 697
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 697
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttatggaa 120
atggccgaac ggatggtgac tgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga ccagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
ggtcctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaagggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccattatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 698
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 698
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Met Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Gly Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 699
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 699
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaggaa 120
atggccgaac gaatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aaccacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggccaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gctatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggagatgc gtgggctgtg 960
gccataatta atcggcaaga gatcggaggt ccacgctcct atacaattcc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacctcac tgaaggacct cctg       1194

 
<210> 700
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 700
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn His Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Pro Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Asp Ala Trp Ala Val 
305                 310                 315                 320 
Ala Ile Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Pro Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 
<210> 701
<211> 1194
<212> DNA
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 701
ctggataatg gattggctag aacacctacc atggggtggc ttcactggga gaggttcatg 60
tgcaacctgg actgtcagga agaaccagac agctgcatct ccgagaagct ctttgaagaa 120
atggccgaac ggatggtgac cgagggctgg aaagatgcag gttacgagta tctgtgtatt 180
gacgattgct ggatggcccc acaacgggat tctgagggaa gacttcaggc tgatccgcag 240
cgcttccctc atggcataag gcagctggca aactacgtcc acagtaaggg gctcaaattg 300
ggaatctacg cggacgtggg caataagacc tgtgccggtt ttccgggatc attcgggtat 360
tatgacattg acgcccaaac gtttgctgat tggggcgttg acctgctgaa attcgatggt 420
tgctactgtg acagcctcga aaacctggca gacggctaca agcatatgtc tctcgccctg 480
aatagaaccg gtcggtcaat cgtatattcc tgcgagtggc ctctttacat gtggccattt 540
cagaaaccga actacacaga aattcgccag tattgcaatc attggaggaa cttcgctgat 600
atcgatgact catgggcctc cataaagagc atcttggact ggaccagtcg gaatcaggag 660
cgaattgtgg atgtcgcagg ccctggagga tggaacgatc cagacatgct ggtgatcggc 720
aattttggcc tctcttggga tcagcaggtt acccaaatgg ctctgtgggc aattatggcc 780
gcccctcttt tcatgagcaa cgatctgcgc gcgatctcac cacaggcaaa ggccctgctc 840
caagacaaag atgtgatagc catcaatcag gacccgttgg gaaagcaggg ctaccagctg 900
agaaaaggcg acaactttga ggtctgggaa aggcctttga gtggactcgc gtgggctgtg 960
gccatgatta atcggcaaga gatcggaggt ccacgctcct atacaattgc tgtagcatct 1020
cttggcaagg gcgttgcctg taacccagct tgcttcatca ctcagcttct gccggtgaag 1080
agaaaactgg gtttctacga agctaccagc aggctccgat cccacatcaa ccctacagga 1140
accgtcctct tgcagctgga gaatacgatg cagacttcac tgaaggacct cctg       1194

 
<210> 702
<211> 398
<212> PRT
<213> Artificial Sequence
 
<220>
<223> variant of human alpha-galactosidase A
 
<400> 702
Leu Asp Asn Gly Leu Ala Arg Thr Pro Thr Met Gly Trp Leu His Trp 
1               5                   10                  15      
Glu Arg Phe Met Cys Asn Leu Asp Cys Gln Glu Glu Pro Asp Ser Cys 
            20                  25                  30          
Ile Ser Glu Lys Leu Phe Glu Glu Met Ala Glu Arg Met Val Thr Glu 
        35                  40                  45              
Gly Trp Lys Asp Ala Gly Tyr Glu Tyr Leu Cys Ile Asp Asp Cys Trp 
    50                  55                  60                  
Met Ala Pro Gln Arg Asp Ser Glu Gly Arg Leu Gln Ala Asp Pro Gln 
65                  70                  75                  80  
Arg Phe Pro His Gly Ile Arg Gln Leu Ala Asn Tyr Val His Ser Lys 
                85                  90                  95      
Gly Leu Lys Leu Gly Ile Tyr Ala Asp Val Gly Asn Lys Thr Cys Ala 
            100                 105                 110         
Gly Phe Pro Gly Ser Phe Gly Tyr Tyr Asp Ile Asp Ala Gln Thr Phe 
        115                 120                 125             
Ala Asp Trp Gly Val Asp Leu Leu Lys Phe Asp Gly Cys Tyr Cys Asp 
    130                 135                 140                 
Ser Leu Glu Asn Leu Ala Asp Gly Tyr Lys His Met Ser Leu Ala Leu 
145                 150                 155                 160 
Asn Arg Thr Gly Arg Ser Ile Val Tyr Ser Cys Glu Trp Pro Leu Tyr 
                165                 170                 175     
Met Trp Pro Phe Gln Lys Pro Asn Tyr Thr Glu Ile Arg Gln Tyr Cys 
            180                 185                 190         
Asn His Trp Arg Asn Phe Ala Asp Ile Asp Asp Ser Trp Ala Ser Ile 
        195                 200                 205             
Lys Ser Ile Leu Asp Trp Thr Ser Arg Asn Gln Glu Arg Ile Val Asp 
    210                 215                 220                 
Val Ala Gly Pro Gly Gly Trp Asn Asp Pro Asp Met Leu Val Ile Gly 
225                 230                 235                 240 
Asn Phe Gly Leu Ser Trp Asp Gln Gln Val Thr Gln Met Ala Leu Trp 
                245                 250                 255     
Ala Ile Met Ala Ala Pro Leu Phe Met Ser Asn Asp Leu Arg Ala Ile 
            260                 265                 270         
Ser Pro Gln Ala Lys Ala Leu Leu Gln Asp Lys Asp Val Ile Ala Ile 
        275                 280                 285             
Asn Gln Asp Pro Leu Gly Lys Gln Gly Tyr Gln Leu Arg Lys Gly Asp 
    290                 295                 300                 
Asn Phe Glu Val Trp Glu Arg Pro Leu Ser Gly Leu Ala Trp Ala Val 
305                 310                 315                 320 
Ala Met Ile Asn Arg Gln Glu Ile Gly Gly Pro Arg Ser Tyr Thr Ile 
                325                 330                 335     
Ala Val Ala Ser Leu Gly Lys Gly Val Ala Cys Asn Pro Ala Cys Phe 
            340                 345                 350         
Ile Thr Gln Leu Leu Pro Val Lys Arg Lys Leu Gly Phe Tyr Glu Ala 
        355                 360                 365             
Thr Ser Arg Leu Arg Ser His Ile Asn Pro Thr Gly Thr Val Leu Leu 
    370                 375                 380                 
Gln Leu Glu Asn Thr Met Gln Thr Ser Leu Lys Asp Leu Leu         
385                 390                 395                     

 


