﻿                         序列表

<110>  丰益（上海）生物技术研发中心有限公司

<120>  用于表达外源基因的毕赤酵母突变株

<130>  188436

<160>  15

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1005
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc attagctgct        60
ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt       120
tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat       180
aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta       240
tctcttgaga aaagagaggc tgaagcttgg tcagctgagg acaagcataa ggaaggtgtg       300
aatagtcact tatggatcgt gaaccgtgcc attgatataa tgtctaggaa tacaactctg       360
gttaagcaag atagagttgc tcaattgaat gaatggcgta cagagctaga gaatggcatc       420
tacgctgctg attatgaaaa cccctattac gataacagta ccttcgcttc tcacttttac       480
gatccagaca acggaaagac atatatccca ttcgccaagc aagctaagga gactggagct       540
aagtacttca agttggctgg agagtcatac aagaataaag acatgaagca ggccttcttt       600
tatcttgggt tgtcattgca ttatttgggc gatgtcaacc aacctatgca tgccgcaaac       660
tttacgaacc tgtcctatcc acagggtttt cactccaagt acgagaactt tgtcgatact       720
attaaagaca actacaaagt taccgatggg aacggatatt ggaattggaa aggcaccaac       780
cctgaagaat ggattcacgg tgcagcagta gttgcaaaac aggactactc tggaattgtc       840
aatgacaata ccaaagattg gtttgtgaaa gccgcagtct cccaggaata tgcagataaa       900
tggagagctg aagttacacc tatgactggt aaacgactaa tggatgccca aagagttact       960
gctggttaca ttcaattatg gttcgacact tacggtgaca ggtaa                      1005

<210>  2
<211>  334
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  2
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      
Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          
Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              
Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  
Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  
Ser Leu Glu Lys Arg Glu Ala Glu Ala Trp Ser Ala Glu Asp Lys His 
                85                  90                  95      
Lys Glu Gly Val Asn Ser His Leu Trp Ile Val Asn Arg Ala Ile Asp 
            100                 105                 110         
Ile Met Ser Arg Asn Thr Thr Leu Val Lys Gln Asp Arg Val Ala Gln 
        115                 120                 125             
Leu Asn Glu Trp Arg Thr Glu Leu Glu Asn Gly Ile Tyr Ala Ala Asp 
    130                 135                 140                 
Tyr Glu Asn Pro Tyr Tyr Asp Asn Ser Thr Phe Ala Ser His Phe Tyr 
145                 150                 155                 160 
Asp Pro Asp Asn Gly Lys Thr Tyr Ile Pro Phe Ala Lys Gln Ala Lys 
                165                 170                 175     
Glu Thr Gly Ala Lys Tyr Phe Lys Leu Ala Gly Glu Ser Tyr Lys Asn 
            180                 185                 190         
Lys Asp Met Lys Gln Ala Phe Phe Tyr Leu Gly Leu Ser Leu His Tyr 
        195                 200                 205             
Leu Gly Asp Val Asn Gln Pro Met His Ala Ala Asn Phe Thr Asn Leu 
    210                 215                 220                 
Ser Tyr Pro Gln Gly Phe His Ser Lys Tyr Glu Asn Phe Val Asp Thr 
225                 230                 235                 240 
Ile Lys Asp Asn Tyr Lys Val Thr Asp Gly Asn Gly Tyr Trp Asn Trp 
                245                 250                 255     
Lys Gly Thr Asn Pro Glu Glu Trp Ile His Gly Ala Ala Val Val Ala 
            260                 265                 270         
Lys Gln Asp Tyr Ser Gly Ile Val Asn Asp Asn Thr Lys Asp Trp Phe 
        275                 280                 285             
Val Lys Ala Ala Val Ser Gln Glu Tyr Ala Asp Lys Trp Arg Ala Glu 
    290                 295                 300                 
Val Thr Pro Met Thr Gly Lys Arg Leu Met Asp Ala Gln Arg Val Thr 
305                 310                 315                 320 
Ala Gly Tyr Ile Gln Leu Trp Phe Asp Thr Tyr Gly Asp Arg 
                325                 330                 

<210>  3
<211>  25
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
tacgtatggt cagctgagga caagc                                              25

<210>  4
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
cctaggttac ctgtcaccgt aagtgtcgaa c                                       31

<210>  5
<211>  1817
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
ctcgagattc aggtgaaccc acctaactat ttttaactgg gatccagtga gctcgctggg        60
tgaaagccaa ccatcttttg tttcggggaa ccgtgctcgc cccgtaaagt taattttttt       120
ttcccgcgca gctttaatct ttcggcagag aaggcgtttt catcgtagcg tgggaacaga       180
ataatcagtt catgtgctat acaggcacat ggcagcagtc actattttgc tttttaacct       240
taaagtcgtt catcaatcat taactgacca atcagatttt ttgcatttgc cacttatcta       300
aaaatacttt tgtatctcgc agatacgttc agtggtttcc aggacaacac ccaaaaaaag       360
gtatcaatgc cactaggcag tcggttttat ttttggtcac ccacgcaaag aagcacccac       420
ctcttttagg ttttaagttg tgggaacagt aacaccgcct agagcttcag gaaaaaccag       480
tacctgtgac cgcaattcac catgatgcag aatgttaatt taaacgagtg ccaaatcaag       540
atttcaacag acaaatcaat cgatccatag ttacccattc cagccttttc gtcgtcgagc       600
ctgcttcatt cctgcctcag gtgcataact ttgcatgaaa agtccagatt agggcagatt       660
ttgagtttaa aataggaaat ataaacaaat ataccgcgaa aaaggtttgt ttatagcttt       720
tcgcctggtg ccgtacggta taaatacata ctctcctccc ccccctggtt ctctttttct       780
tttgttactt acattttacc gttccgtcac tcgcttcact caacaacaaa ataaactagt       840
ggtaccgtca gccaccaaag tagtgaatag accatcgggg cggtcagtag tcaaagacgc       900
caacaaaatt tcactgacag ggaacttttt gacatcttca gaaagttcgt attcagtagt       960
caattgccga gcatcaataa tggggattat accagaagca acagtggaag tcacatctac      1020
caactttgcg gtctcagaaa aagcataaac agttctacta ccgccattag tgaaactttt      1080
caaatcgccc agtggagaag aaaaaggcac agcgatacta gcattagcgg gcaaggatgc      1140
aactttatca accagggtcc tatagataac cctagcgcct gggatcatcc tttggacaac      1200
tctttctgcc aaatctaggt ccaaaatcac ttcattgata ccattattgt acaacttgag      1260
caagttgtcg atcagctcct caaattggtc ctctgtaacg gatgactcaa cttgcacatt      1320
aacttgaagc tcagtcgatt gagtgaactt gatcaggttg tgcagctggt cagcagcata      1380
gggaaacacg gcttttccta ccaaactcaa ggaattatca aactctgcaa cacttgcgta      1440
tgcaggtagc aagggaaatg tcatacttga agtcggacag tgagtgtagt cttgagaaat      1500
tctgaagccg tatttttatt atcagtgagt cagtcatcag gagatcctct acgccggacg      1560
catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat      1620
caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg      1680
tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt      1740
ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga      1800
gtcgcataag ggaattc                                                     1817

<210>  6
<211>  1038
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
gttccaatca agagacaatc taattccact gtcgattctt tgcctccatt gattccttct        60
agaactagtg caccttcatc ctctccatct acaactgacc ctgaggctcc agctatgtca       120
agaaatggtc cacttccttc tgatgttgag accaagtacg gaatggccct gaatgctact       180
tcttatccag attctgtcgt tcaagctatg aaaagagagg ctgaagcttc catcgacgga       240
ggtattagag ccgctacttc tcaggaaatc aacgaactta cttactatac aactttgtca       300
gctaattctt actgtagaac tgttattcct ggtgctactt gggattgcat acattgtgac       360
gccactgaag atttaaagat aattaaaacc tggtctactt tgatttacga cactaacgct       420
atggttgcta gaggagattc cgagaagact atttatatcg tgtttagagg ttcttcatct       480
attcgtaatt ggatcgctga tttgacattc gttccagtct cttaccctcc agtttctggt       540
actaaggttc acaaaggatt tcttgattct tatggtgaag ttcaaaacga gttggttgct       600
actgtcttgg atcagtttaa acaataccca tcttataagg ttgctgtcac tggtcactct       660
ttgggaggtg ctactgcctt gctgtgtgct ttaggtttat accagagaga ggaaggattg       720
tcttcaagta acctattctt gtacactcaa ggtcagccta gagttggaga tccagcattt       780
gctaattatg tggtttctac tggtattcca tatagacgta ctgttaacga aagagacata       840
gtaccacact tgcctccagc tgccttcgga tttctgcatg ccggtgaaga gtactggatc       900
acagataatt ctcctgaaac cgttcaagtg tgtacatctg atttagagac ttccgactgc       960
tctaacagta ttgttccatt tacttcagtt cttgatcatt tgtcttattt tggaattaac      1020
accggtttgt gtacttaa                                                    1038

<210>  7
<211>  345
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  7
Val Pro Ile Lys Arg Gln Ser Asn Ser Thr Val Asp Ser Leu Pro Pro 
1               5                   10                  15      
Leu Ile Pro Ser Arg Thr Ser Ala Pro Ser Ser Ser Pro Ser Thr Thr 
            20                  25                  30          
Asp Pro Glu Ala Pro Ala Met Ser Arg Asn Gly Pro Leu Pro Ser Asp 
        35                  40                  45              
Val Glu Thr Lys Tyr Gly Met Ala Leu Asn Ala Thr Ser Tyr Pro Asp 
    50                  55                  60                  
Ser Val Val Gln Ala Met Lys Arg Glu Ala Glu Ala Ser Ile Asp Gly 
65                  70                  75                  80  
Gly Ile Arg Ala Ala Thr Ser Gln Glu Ile Asn Glu Leu Thr Tyr Tyr 
                85                  90                  95      
Thr Thr Leu Ser Ala Asn Ser Tyr Cys Arg Thr Val Ile Pro Gly Ala 
            100                 105                 110         
Thr Trp Asp Cys Ile His Cys Asp Ala Thr Glu Asp Leu Lys Ile Ile 
        115                 120                 125             
Lys Thr Trp Ser Thr Leu Ile Tyr Asp Thr Asn Ala Met Val Ala Arg 
    130                 135                 140                 
Gly Asp Ser Glu Lys Thr Ile Tyr Ile Val Phe Arg Gly Ser Ser Ser 
145                 150                 155                 160 
Ile Arg Asn Trp Ile Ala Asp Leu Thr Phe Val Pro Val Ser Tyr Pro 
                165                 170                 175     
Pro Val Ser Gly Thr Lys Val His Lys Gly Phe Leu Asp Ser Tyr Gly 
            180                 185                 190         
Glu Val Gln Asn Glu Leu Val Ala Thr Val Leu Asp Gln Phe Lys Gln 
        195                 200                 205             
Tyr Pro Ser Tyr Lys Val Ala Val Thr Gly His Ser Leu Gly Gly Ala 
    210                 215                 220                 
Thr Ala Leu Leu Cys Ala Leu Gly Leu Tyr Gln Arg Glu Glu Gly Leu 
225                 230                 235                 240 
Ser Ser Ser Asn Leu Phe Leu Tyr Thr Gln Gly Gln Pro Arg Val Gly 
                245                 250                 255     
Asp Pro Ala Phe Ala Asn Tyr Val Val Ser Thr Gly Ile Pro Tyr Arg 
            260                 265                 270         
Arg Thr Val Asn Glu Arg Asp Ile Val Pro His Leu Pro Pro Ala Ala 
        275                 280                 285             
Phe Gly Phe Leu His Ala Gly Glu Glu Tyr Trp Ile Thr Asp Asn Ser 
    290                 295                 300                 
Pro Glu Thr Val Gln Val Cys Thr Ser Asp Leu Glu Thr Ser Asp Cys 
305                 310                 315                 320 
Ser Asn Ser Ile Val Pro Phe Thr Ser Val Leu Asp His Leu Ser Tyr 
                325                 330                 335     
Phe Gly Ile Asn Thr Gly Leu Cys Thr 
            340                 345 

<210>  8
<211>  810
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
gaagtctctc aagacttgtt caaccagttc aacttgttcg ctcaatactc tgccgctgcc        60
tactgtggta agaacaatga tgctccagct ggtactaaca ttacctgtac tggtaacgct       120
tgtccagaag ttgagaaggc tgatgctacc ttcctgtact ccttcgaaga ctctggagtt       180
ggagatgtta ctggtttcct ggccttggat aacactaaca agttgatcgt tctgtccttc       240
agaggttcca gatccatcga gaactggatt ggtaacttga actttgactt gaaggagatc       300
aacgacatct gttctggatg tcgtggtcac gatggattta cctcctcttg gagatctgtt       360
gctgatacct tgagacagaa ggtcgaagat gctgtcagag aacatccaga ctatagagtt       420
gtcttcactg gtcactcctt gggaggtgcc ttggctactg ttgctggtgc tgacttgcgt       480
ggtaatggtt atgacattga tgtcttctcc tacggtgctc caagagttgg taatcgtgcc       540
ttcgctgagt ttctgaccgt ccaaactgga ggtactttgt acagaattac ccatactaac       600
gacattgttc caagattgcc accacgtgag ttcggatact ctcattcctc tccagagtac       660
tggatcaagt ctggaacctt ggttccagtc actcgtaacg acatcgtcaa gattgaaggt       720
attgatgcca ctggaggtaa caatcaacca aacattccag acattccagc tcacttgtgg       780
tactttggtc tgattggtac ttgcttgtaa                                        810

<210>  9
<211>  269
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  9
Glu Val Ser Gln Asp Leu Phe Asn Gln Phe Asn Leu Phe Ala Gln Tyr 
1               5                   10                  15      
Ser Ala Ala Ala Tyr Cys Gly Lys Asn Asn Asp Ala Pro Ala Gly Thr 
            20                  25                  30          
Asn Ile Thr Cys Thr Gly Asn Ala Cys Pro Glu Val Glu Lys Ala Asp 
        35                  40                  45              
Ala Thr Phe Leu Tyr Ser Phe Glu Asp Ser Gly Val Gly Asp Val Thr 
    50                  55                  60                  
Gly Phe Leu Ala Leu Asp Asn Thr Asn Lys Leu Ile Val Leu Ser Phe 
65                  70                  75                  80  
Arg Gly Ser Arg Ser Ile Glu Asn Trp Ile Gly Asn Leu Asn Phe Asp 
                85                  90                  95      
Leu Lys Glu Ile Asn Asp Ile Cys Ser Gly Cys Arg Gly His Asp Gly 
            100                 105                 110         
Phe Thr Ser Ser Trp Arg Ser Val Ala Asp Thr Leu Arg Gln Lys Val 
        115                 120                 125             
Glu Asp Ala Val Arg Glu His Pro Asp Tyr Arg Val Val Phe Thr Gly 
    130                 135                 140                 
His Ser Leu Gly Gly Ala Leu Ala Thr Val Ala Gly Ala Asp Leu Arg 
145                 150                 155                 160 
Gly Asn Gly Tyr Asp Ile Asp Val Phe Ser Tyr Gly Ala Pro Arg Val 
                165                 170                 175     
Gly Asn Arg Ala Phe Ala Glu Phe Leu Thr Val Gln Thr Gly Gly Thr 
            180                 185                 190         
Leu Tyr Arg Ile Thr His Thr Asn Asp Ile Val Pro Arg Leu Pro Pro 
        195                 200                 205             
Arg Glu Phe Gly Tyr Ser His Ser Ser Pro Glu Tyr Trp Ile Lys Ser 
    210                 215                 220                 
Gly Thr Leu Val Pro Val Thr Arg Asn Asp Ile Val Lys Ile Glu Gly 
225                 230                 235                 240 
Ile Asp Ala Thr Gly Gly Asn Asn Gln Pro Asn Ile Pro Asp Ile Pro 
                245                 250                 255     
Ala His Leu Trp Tyr Phe Gly Leu Ile Gly Thr Cys Leu 
            260                 265                 

<210>  10
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
ccgctcgagt cacctcagcc agatcaaagt                                         30

<210>  11
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
acatgcatgc ctttggacaa ctctttctgc c                                       31

<210>  12
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
cggggtaccc ctggttgata aagttgcat                                          29

<210>  13
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
ggcgagctca ggtgtcttca aagcgactc                                          29

<210>  14
<211>  35
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
acatgcatgc ctgcagaaat ggggagataa ccacc                                   35

<210>  15
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  15
cggggtacca ctagtggttt tctgggggta tttgctg                                 37

